Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0003601

Physical Location & Seq

Genomic Coordinates Reverse (-)

61607670 .. 61616668

Gene Structure 8999 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ59720

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3294 bp

ATGTCCAAAGAAAACGGTCAAATCCACGGCGGAGGCGGACTCACGGAGGAGCAAAGAAACCGAATTTCTCGGAATTACAGAGCCGCCAAGGCTCTTCTCGACCGTAAACGCTCTCGTAACGACGCCGTTTCGCCTGATGCGCACTTCCCTCACAGCAAATTCCGAACTCCTACTCAGCTTAATCGGCGAGCTCCTCTTGCTGAGCTAACGGTAAATACTCCATCTCCATTTTCTGTGAATCGCTGCAACTCAGCAGTTTCTGAATCCAGCACCGGTTCATATTGTGAGAGCTCAAGCTCAGTCATTACTCGGATTGAAGATTCGTTCACTACTCCGATCAGGCTACCGCAGTTTTGTACTCCAGTTGATTCTTTCTCGGCGGCGACCGGAATTCTGGACGACGACGATGATTTCGATGAGAGTGTTTTGCAGGAGATTGACGCTTTGATCGAGCAGAAAACTCTACAAGTTCAGCGTGATGTTAATGTGAATGGTTCTGTGGTGGCGCGTGTGATTAGTGGCGGTGAAGGTGATTCTACTGGCCATGGATTACCGAGCATGCCGGATGAGTACTTGAAGTATTTACAGTCTCTCAATGACCGGCAACGCGAAGCAGCTTGTAGTGACATTGCAACACCATTGATGATTGCTGCAGGACCTGGAAGTGGAAAGACCTCAACTATGGTTGGGCGTGTTCTAATGCTGCTTAATGAGGGGATTAGTGCATCCAACATTCTAGCAATGACTTTCACTATAGCAGCAGCTTCCGAGATGAGAGAGCGCATTGCAAAGGTGGCTGGGAAAGCAGCGGCAAAAGAACTTACAATCAGCACCTTTCATTCATTTTCATTGCAGCTTTGTCGATTACATGCAGAGAAACTTGAGCGCACACCAGAGTTCTTAATATATGGACATGGACAACAGAGAAGAGCAATTATTGAAGCTGTGCGGTTATCAGAAAATGGAAAGAGTTGGCCGAAACATAATGTCGGCATGGACAGTGAAGAGTCTAATGGTTTAACTTCTCCTCATCACTTTAAGGATGCGTCAAAGAAATGGCTGAAATTTGTAACTCAGGCCAAAGCTTCTGGAAAGACTCCTGCAGAATACAGTAAAATGGGCAATGAGATAGGGGCTGAGATTCTTGGAAACTACAATGATATCTTAAAATCTTGCAATGCCTTGGACTACCATGACTTGATTAGCTGCTCTGTGAAGCTGCTCATGGATTTTCCTGAAGTGTTAAAGGAGAGCCAGGATTCATGGAAAGCCATTGTCATAGATGAGTTTCAAGACACAAGTGCTATGCAATATAGTCTTTTGCGGATTCTTGCCTCTCACAACCACATAACCATTGTTGGAGATGATGATCAATCGATTTTCAGTTTCAATGGAGCAGACATTTCTGGATTTGATTCCTTCCGTAAGGATTTCCCCATTTACAAAGAGATTAGACTCAATAAAAACTATCGGTCTACACGCTATATTGTGGAAGCTGCATCATGTGTCATAAAAAACAATAAGAACAGATGTCAGCTAAAGAATGTTGACACTGACAACTCTTCTGGATCCAAGATTGTTATCAAAGAATGCCACAATGAGGATGCACAATGTGCCTTTGTTGTTGACAAGATCTTGGAGACCACATCTAACCAATCAAATGTAAAGTGCTCATTCGGAAACATCGCAATCCTTTACCGGAGGCAGGTATCAGGAAAAGTCTTCCAAACAGCCTTTCGTGAAAGGAAAATACCTTTTAATGTTCATGGAGTAGCAGTTTATCGGAAGAAGGTAGTTAGAGCCATTATTGCTATGCTTAGAACAACTTTGCCTGGTTGTGATGATGGATCATATCGCCGAGTCTTCAAGGCTTTACTTCCTTTTGAGAAAGAGGAAAAGAAGAAGGTGATTGATTACATTGACAAAATTTCTACCGCCAGAAAATTCAGCTTCATATCAGCTGCTGGTGAAATTTTCAGTGCAAAAGTTTCTGGGAACTTGAAGAGGCCTCAGCTTACTCAAGGACGCAAGGTGCTGTTGACATTAGAGATGATTTCAAAACTCGTTCACAGGGAACAATCAATTTCAGCTGTCATAACCTCAGTGGCGAACATGGTACCACAGAAATACCTTCTGGAGCAGCGTGCTATCGTCGATGTTGATGGTGGGAAGTTGCTAAATGAAGACAATGACATGAGATCTGTTCTTCAGTACCTACTGGATGATATATCTGATTTTCTATCCACTCATTTTGCTGCGGCAGAAGGGGAGAGAGAAGTTGTAGAAGGAGATAAAGGATGTCTTAATTTACTCAAAGCTTTCATTGACTACATATCTGAGCGGGAGAGTGAAAACTTTCGCTCCCGAAGACATGACAATGAAACTTCTGTTACCTTAACTACCATCCATCAGTCAAAAGGTTTAGAATGGGACATTGTTTTCATAATTAAGGTAAATGAATGTGAGATACCGTTGTTGCATGAATTCAATGGTAGTGCAAAAGAGAATGGGGCGTCCATTGAGGAGGAAAGACGCCTATTATATGTTGCAATGACTCGTGCTCGAACAAAACTTTTCATTCTTTATGTTTTGACGGATTCCAATTGGCAGATGCTTCAACCTTCACGGTTTCTCAGAGAAATTCCAAATCATCTTCGAGAAGAACAGGCTGACATAAAAAAGCAAGATTTACAGTCAAAGCATCATGACATTTCAAAAGGAACTACTGGGTTGTCGGCTGATCTACGACCAAACATTCGACCCTCTGAAGAAGATGTGGTGCAGAATGATTTTCACCCTAGCAAAATTGATGAAGCTTCTACAGAGTTGACTGAATTTGTGGAGGCAAACTTTGGAACTGGTTTCTTAAAAAGATTCTGTGTGGACGAGAGATCAATTATCTCACATTTATTTAATGAGTGGGCTAAGAAGCAAGCATTTCAACATCCTAAGAGGTTGCTTGACAAGGTGGGTTTTGTAATAGATGAACGTCTGCGAGTCAAGAACTACAAACACAAGGATGTGTTGCGCTTGCTGAAGTCTTACCTGAAAGGTGACGATGCATTTCAATATGCGGAATACGTTTTAAGATGGCAGAAAATACCTGCTGATCAACGTGCTCATATGATGCGGGAAAAGCAGGAGCATTTCCAGAAGTTAAGGATTGAGAACTCAATGGGTTCATCTGCACCAACACCGAAACAGATTTCTTATCTGCAAAGTTTGGGTTGCACCGTGGCTCCGACGTCGCGCCTCCATGCTTCTCGTTTGATTGAGCAGTACAAGTCACTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003678 GO:0003824 GO:0004386 GO:0006139 GO:0006259 GO:0006310 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017111 GO:0032392 GO:0032508 GO:0034641 GO:0036292 GO:0043138 GO:0043170 GO:0044237 GO:0044238 GO:0044260 GO:0046483 GO:0051276 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:0140097 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

UvrD-helicase UvrD_C

Protein Analysis

1097

Amino Acids

123.71

Weight (kDa)

8.33

Isoelectric Point (pI)

43.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
UvrD-helicase	PF00580	198 - 479	1e-67	UvrD/REP helicase N-terminal domain
AAA_19	PF13245	202 - 463	6.3e-22	AAA domain
UvrD_C	PF13361	484 - 863	5.3e-45	UvrD-like helicase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003601)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G25120
fragaria_vesca	FvH4_7g26710 FvH4_7g26710 FvH4_7g26710 FvH4_7g26710
malus_domestica	MD07G1241600.v1.1 MD07G1241700.v1.1 MD07G1243100.v1.1 MD07G1243600.v1.1 MD07G1244100.v1.1 MD07G1244200.v1.1 MD07G1244300.v1.1 MD07G1244800.v1.1 MD07G1245200.v1.1 MD07G1245700.v1.1 MD07G1245800.v1.1
prunus_persica	Prupe.2G270600_v2.0.a1 Prupe.2G270600_v2.0.a1
pyrus_communis	pycom07g21810 pycom07g21980
rosa_chinensis	RchiOBHm_Chr1g0373301
rosa_laevigata	RLG00000001620 RLG00000006922 RLG00000035899
rosa_multiflora	Rmu_ssc0000435.1_g000004
rosa_roxburghii	Rroxscaffold_4G00284280
rosa_rugosa	Rorug01G0376900
rosa_samantha	Rh1AG385900 Rh1BG350100 Rh1CG363200 Rh1DG380900 Rh3CG316100 Rh5CG053300 Rh5DG386500
rosa_wichuraiana	Rw1G034900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	3248
Acc16I	TGCGCA	1 cut(s)	141
Acc36I	ACCTGC	2 cut(s)	1696, 3112
Acc65I	GGTACC	1 cut(s)	2116
AccB1I	GGYRCC	1 cut(s)	2116
AccBSI	CCGCTC	1 cut(s)	2341
AccI	GTMKAC	1 cut(s)	1475
AccII	CGCG	3 cut(s)	508, 609, 3250
AclWI	GGATC	3 cut(s)	1563, 1576, 1855
AcoI	YGGCCR	2 cut(s)	541, 974
AcuI	CTGAAG	4 cut(s)	1257, 2192, 2787, 3056
AcyI	GRCGYC	4 cut(s)	123, 2513, 2533, 3245
AdeI	CACNNNGTG	1 cut(s)	1613
AfaI	GTAC	5 cut(s)	358, 572, 2118, 2213, 3281
AfiI	CCNNNNNNNGG	3 cut(s)	665, 1600, 1705
AgeI	ACCGGT	1 cut(s)	272
AjnI	CCWGG	3 cut(s)	658, 1254, 1831
AjuI	GAANNNNNNNTTGG	2 cut(s)	1719, 1751
Alw21I	GWGCWC	5 cut(s)	193, 293, 1673, 2563, 3121
Alw26I	GTCTC	2 cut(s)	594, 1634
AlwI	GGATC	3 cut(s)	1563, 1576, 1855
AlwNI	CAGNNNCTG	3 cut(s)	260, 659, 1964
AoxI	GGCC	4 cut(s)	541, 974, 1077, 2006
ArsI	GACNNNNNNTTYG	4 cut(s)	395, 427, 2739, 2771
AsiGI	ACCGGT	1 cut(s)	272
Asp700I	GAANNNNTTC	1 cut(s)	2355
Asp718I	GGTACC	1 cut(s)	2116
AspLEI	GCGC	6 cut(s)	142, 508, 783, 888, 3030, 3252
AspS9I	GGNCC	1 cut(s)	656
AsuHPI	GGTGA	6 cut(s)	536, 542, 1918, 1979, 2786, 3065
AvaII	GGWCC	1 cut(s)	656
BaeI	ACNNNNGTAYC	2 cut(s)	2459, 2492
BalI	TGGCCA	1 cut(s)	543
BamHI	GGATCC	1 cut(s)	1568
BanI	GGYRCC	1 cut(s)	2116
BanII	GRGCYC	2 cut(s)	193, 293
BauI	CACGAG	1 cut(s)	2556
BbsI	GAAGAC	4 cut(s)	1714, 1855, 2190, 2374
Bbv12I	GWGCWC	5 cut(s)	193, 293, 1673, 2563, 3121
BbvCI	CCTCAGC	1 cut(s)	2010
BccI	CCATC	6 cut(s)	229, 1838, 2156, 2411, 2415, 3084
BceAI	ACGGC	2 cut(s)	43, 110
BcgI	CGANNNNNNTGC	2 cut(s)	842, 876
BciT130I	CCWGG	3 cut(s)	660, 1256, 1833
BclI	TGATCA	2 cut(s)	1369, 3109
BcoDI	GTCTC	2 cut(s)	594, 1634
BfaI	CTAG	2 cut(s)	737, 2799
BfmI	CTRYAG	4 cut(s)	651, 753, 1101, 2820
BfuAI	ACCTGC	2 cut(s)	1696, 3112
BglI	GCCNNNNNGGC	1 cut(s)	89
BglII	AGATCT	2 cut(s)	1632, 2198
BlpI	GCTNAGC	1 cut(s)	201
BmcAI	AGTACT	1 cut(s)	572
Bme1390I	CCNGG	3 cut(s)	660, 1256, 1833
Bme18I	GGWCC	1 cut(s)	656
BmgT120I	GGNCC	1 cut(s)	656
BmiI	GGNNCC	3 cut(s)	1570, 2118, 3240
BmrFI	CCNGG	3 cut(s)	660, 1256, 1833
BmrI	ACTGGG	1 cut(s)	2736
BmsI	GCATC	9 cut(s)	127, 734, 1033, 1508, 1594, 2601, 2710, 3049, 3117
BmuI	ACTGGG	1 cut(s)	2736
BpiI	GAAGAC	4 cut(s)	1714, 1855, 2190, 2374
BplI	GAGNNNNNCTC	2 cut(s)	24, 56
BpmI	CTGGAG	2 cut(s)	345, 2156
Bpu10I	CCTNAGC	1 cut(s)	2010
Bpu1102I	GCTNAGC	1 cut(s)	201
BpuEI	CTTGAG	3 cut(s)	277, 902, 2004
Bsa29I	ATCGAT	1 cut(s)	1376
BsaBI	GATNNNNATC	2 cut(s)	1368, 1580
BsaHI	GRCGYC	4 cut(s)	123, 2513, 2533, 3245
BsaI	GGTCTC	1 cut(s)	1634
BsaJI	CCNNGG	5 cut(s)	25, 87, 544, 1182, 3234
BsaWI	WCCGGW	3 cut(s)	272, 386, 1698
BsaXI	ACNNNNNCTCC	8 cut(s)	208, 238, 1761, 1791, 2345, 2375, 3223, 3253
Bsc4I	CCNNNNNNNGG	3 cut(s)	665, 1600, 1705
Bse118I	RCCGGY	2 cut(s)	272, 600
Bse1I	ACTGG	5 cut(s)	362, 544, 2223, 2731, 2863
Bse3DI	GCAATG	7 cut(s)	627, 747, 783, 848, 1129, 1183, 2556
Bse8I	GATNNNNATC	2 cut(s)	1368, 1580
BseBI	CCWGG	3 cut(s)	660, 1256, 1833
BseCI	ATCGAT	1 cut(s)	1376
BseDI	CCNNGG	5 cut(s)	25, 87, 544, 1182, 3234
BseGI	GGATG	9 cut(s)	571, 725, 1048, 1609, 2227, 2303, 2403, 2944, 3025
BseJI	GATNNNNATC	2 cut(s)	1368, 1580
BseLI	CCNNNNNNNGG	3 cut(s)	665, 1600, 1705
BseMI	GCAATG	7 cut(s)	627, 747, 783, 848, 1129, 1183, 2556
BseNI	ACTGG	5 cut(s)	362, 544, 2223, 2731, 2863
BseRI	GAGGAG	4 cut(s)	62, 183, 1017, 2537
BseYI	CCCAGC	1 cut(s)	797
BsgI	GTGCAG	2 cut(s)	2801, 3171
Bsh1236I	CGCG	3 cut(s)	508, 609, 3250
Bsh1285I	CGRYCG	2 cut(s)	103, 387
BshFI	GGCC	4 cut(s)	543, 976, 1079, 2008
BshNI	GGYRCC	1 cut(s)	2116
BshTI	ACCGGT	1 cut(s)	272
BshVI	ATCGAT	1 cut(s)	1376
BsiEI	CGRYCG	2 cut(s)	103, 387
BsiHKAI	GWGCWC	5 cut(s)	193, 293, 1673, 2563, 3121
BsiSI	CCGG	5 cut(s)	273, 387, 563, 601, 1699
BslFI	GGGAC	1 cut(s)	2444
BslI	CCNNNNNNNGG	3 cut(s)	665, 1600, 1705
BsmAI	GTCTC	2 cut(s)	594, 1634
BsmFI	GGGAC	1 cut(s)	2444
BsmI	GAATGC	1 cut(s)	1595
BsnI	GGCC	4 cut(s)	543, 976, 1079, 2008
Bso31I	GGTCTC	1 cut(s)	1634
Bsp1286I	GDGCHC	5 cut(s)	193, 293, 1673, 2563, 3121
Bsp1720I	GCTNAGC	1 cut(s)	201
Bsp19I	CCATGG	1 cut(s)	544
BspANI	GGCC	4 cut(s)	543, 976, 1079, 2008
BspDI	ATCGAT	1 cut(s)	1376
BspFNI	CGCG	3 cut(s)	508, 609, 3250
BspHI	TCATGA	1 cut(s)	2704
BspLI	GGNNCC	3 cut(s)	1570, 2118, 3240
BspMAI	CTGCAG	2 cut(s)	655, 1105
BspMI	ACCTGC	2 cut(s)	1696, 3112
BspPI	GGATC	3 cut(s)	1563, 1576, 1855
BspQI	GCTCTTC	2 cut(s)	99, 922
BspT107I	GGYRCC	1 cut(s)	2116
BspTNI	GGTCTC	1 cut(s)	1634
BsrBI	CCGCTC	1 cut(s)	2341
BsrDI	GCAATG	7 cut(s)	627, 747, 783, 848, 1129, 1183, 2556
BsrFI	RCCGGY	2 cut(s)	272, 600
BsrI	ACTGG	5 cut(s)	362, 544, 2223, 2731, 2863
BssAI	RCCGGY	2 cut(s)	272, 600
BssECI	CCNNGG	5 cut(s)	25, 87, 544, 1182, 3234
BssNI	GRCGYC	4 cut(s)	123, 2513, 2533, 3245
BssSI	CACGAG	1 cut(s)	2556
BssT1I	CCWWGG	3 cut(s)	87, 544, 1182
Bst2BI	CACGAG	1 cut(s)	2556
Bst2UI	CCWGG	3 cut(s)	660, 1256, 1833
Bst6I	CTCTTC	5 cut(s)	99, 922, 999, 1567, 1997
BstACI	GRCGYC	4 cut(s)	123, 2513, 2533, 3245
BstAPI	GCANNNNNTGC	1 cut(s)	1613
BstC8I	GCNNGC	5 cut(s)	189, 560, 2145, 2934, 3032
BstDSI	CCRYGG	3 cut(s)	25, 544, 3234
BstF5I	GGATG	9 cut(s)	571, 725, 1048, 1609, 2227, 2303, 2403, 2944, 3025
BstFNI	CGCG	3 cut(s)	508, 609, 3250
BstHHI	GCGC	6 cut(s)	142, 508, 783, 888, 3030, 3252
BstMAI	GTCTC	2 cut(s)	594, 1634
BstMCI	CGRYCG	2 cut(s)	103, 387
BstMWI	GCNNNNNNNGC	9 cut(s)	89, 139, 184, 197, 794, 803, 1613, 1808, 3136
BstNI	CCWGG	3 cut(s)	660, 1256, 1833
BstNSI	RCATGY	2 cut(s)	562, 872
BstSCI	CCNGG	3 cut(s)	658, 1254, 1831
BstSFI	CTRYAG	4 cut(s)	651, 753, 1101, 2820
BstUI	CGCG	3 cut(s)	508, 609, 3250
BstV2I	GAAGAC	4 cut(s)	1714, 1855, 2190, 2374
BstX2I	RGATCY	3 cut(s)	1568, 1632, 2198
BstYI	RGATCY	3 cut(s)	1568, 1632, 2198
Bsu15I	ATCGAT	1 cut(s)	1376
BsuRI	GGCC	4 cut(s)	543, 976, 1079, 2008
BsuTUI	ATCGAT	1 cut(s)	1376
BtgI	CCRYGG	3 cut(s)	25, 544, 3234
BtgZI	GCGATG	1 cut(s)	1669
BtsCI	GGATG	9 cut(s)	571, 725, 1048, 1609, 2227, 2303, 2403, 2944, 3025
BtsIMutI	CAGTG	5 cut(s)	1006, 1551, 1984, 2109, 3287
BveI	ACCTGC	2 cut(s)	1696, 3112
Cac8I	GCNNGC	5 cut(s)	189, 560, 2145, 2934, 3032
CaiI	CAGNNNCTG	3 cut(s)	260, 659, 1964
CciI	TCATGA	1 cut(s)	2704
CfoI	GCGC	6 cut(s)	142, 508, 783, 888, 3030, 3252
Cfr10I	RCCGGY	2 cut(s)	272, 600
Cfr13I	GGNCC	1 cut(s)	656
ClaI	ATCGAT	1 cut(s)	1376
CseI	GACGC	6 cut(s)	131, 449, 1035, 2034, 2502, 2541
Csp6I	GTAC	5 cut(s)	357, 571, 2117, 2212, 3280
CspAI	ACCGGT	1 cut(s)	272
CspCI	CAANNNNNGTGG	2 cut(s)	2233, 2268
CviQI	GTAC	5 cut(s)	357, 571, 2117, 2212, 3280
DraIII	CACNNNGTG	1 cut(s)	1613
EaeI	YGGCCR	2 cut(s)	541, 974
Eam1104I	CTCTTC	5 cut(s)	99, 922, 999, 1567, 1997
EarI	CTCTTC	5 cut(s)	99, 922, 999, 1567, 1997
EciI	GGCGGA	2 cut(s)	45, 51
Ecl136II	GAGCTC	2 cut(s)	191, 291
Eco130I	CCWWGG	3 cut(s)	87, 544, 1182
Eco147I	AGGCCT	1 cut(s)	2008
Eco24I	GRGCYC	2 cut(s)	193, 293
Eco31I	GGTCTC	1 cut(s)	1634
Eco32I	GATATC	1 cut(s)	1162
Eco47I	GGWCC	1 cut(s)	656
Eco53kI	GAGCTC	2 cut(s)	191, 291
Eco57I	CTGAAG	4 cut(s)	1257, 2192, 2787, 3056
EcoICRI	GAGCTC	2 cut(s)	191, 291
EcoO109I	RGGNCCY	1 cut(s)	656
EcoRI	GAATTC	2 cut(s)	390, 2483
EcoRII	CCWGG	3 cut(s)	658, 1254, 1831
EcoRV	GATATC	1 cut(s)	1162
EcoT14I	CCWWGG	3 cut(s)	87, 544, 1182
EcoT22I	ATGCAT	1 cut(s)	3064
EcoT38I	GRGCYC	2 cut(s)	193, 293
ErhI	CCWWGG	3 cut(s)	87, 544, 1182
FalI	AAGNNNNNCTT	2 cut(s)	2286, 2318
FaqI	GGGAC	1 cut(s)	2444
FauI	CCCGC	2 cut(s)	2334, 3123
FauNDI	CATATG	1 cut(s)	3123
FbaI	TGATCA	2 cut(s)	1369, 3109
FblI	GTMKAC	1 cut(s)	1475
FokI	GGATG	9 cut(s)	578, 712, 1055, 1616, 2234, 2310, 2390, 2931, 3032
FriOI	GRGCYC	2 cut(s)	193, 293
FspAI	RTGCGCAY	1 cut(s)	141
FspBI	CTAG	2 cut(s)	737, 2799
FspI	TGCGCA	1 cut(s)	141
GlaI	GCGC	6 cut(s)	141, 507, 782, 887, 3029, 3251
GsaI	CCCAGC	1 cut(s)	801
GsuI	CTGGAG	2 cut(s)	345, 2156
HaeIII	GGCC	4 cut(s)	543, 976, 1079, 2008
HapII	CCGG	5 cut(s)	273, 387, 563, 601, 1699
HgaI	GACGC	6 cut(s)	131, 449, 1035, 2034, 2502, 2541
HhaI	GCGC	6 cut(s)	142, 508, 783, 888, 3030, 3252
Hin1I	GRCGYC	4 cut(s)	123, 2513, 2533, 3245
Hin6I	GCGC	6 cut(s)	140, 506, 781, 886, 3028, 3250
HinP1I	GCGC	6 cut(s)	140, 506, 781, 886, 3028, 3250
HincII	GTYRAC	4 cut(s)	1549, 1627, 2040, 2829
HindII	GTYRAC	4 cut(s)	1549, 1627, 2040, 2829
HindIII	AAGCTT	3 cut(s)	1083, 2316, 2814
HpaII	CCGG	5 cut(s)	273, 387, 563, 601, 1699
HphI	GGTGA	6 cut(s)	536, 542, 1918, 1979, 2786, 3065
Hpy166II	GTNNAC	9 cut(s)	107, 327, 1476, 1549, 1627, 2040, 2068, 2829, 2884
Hpy8I	GTNNAC	9 cut(s)	107, 327, 1476, 1549, 1627, 2040, 2068, 2829, 2884
Hpy99I	CGWCG	6 cut(s)	125, 404, 407, 2156, 3247, 3250
HpyAV	CCTTC	8 cut(s)	521, 1429, 1783, 1897, 2141, 2258, 2279, 2631
HpyCH4IV	ACGT	4 cut(s)	2989, 3081, 3115, 3245
HpyF10VI	GCNNNNNNNGC	9 cut(s)	89, 139, 184, 197, 794, 803, 1613, 1808, 3136
HpySE526I	ACGT	4 cut(s)	2989, 3081, 3115, 3245
Hsp92I	GRCGYC	4 cut(s)	123, 2513, 2533, 3245
HspAI	GCGC	6 cut(s)	140, 506, 781, 886, 3028, 3250
KpnI	GGTACC	1 cut(s)	2120
Ksp22I	TGATCA	2 cut(s)	1369, 3109
LguI	GCTCTTC	2 cut(s)	99, 922
LmnI	GCTCC	7 cut(s)	49, 196, 1394, 2137, 2366, 3142, 3244
LweI	GCATC	9 cut(s)	127, 734, 1033, 1508, 1594, 2601, 2710, 3049, 3117
MaeI	CTAG	2 cut(s)	737, 2799
MaeII	ACGT	4 cut(s)	2989, 3081, 3115, 3245
MaeIII	GTNAC	6 cut(s)	116, 623, 1069, 2389, 3053, 3285
MbiI	CCGCTC	1 cut(s)	2341
MfeI	CAATTG	1 cut(s)	2602
MflI	RGATCY	3 cut(s)	1568, 1632, 2198
MhlI	GDGCHC	5 cut(s)	193, 293, 1673, 2563, 3121
MlsI	TGGCCA	1 cut(s)	543
MluNI	TGGCCA	1 cut(s)	543
MlyI	GAGTC	7 cut(s)	33, 1016, 1090, 1449, 1869, 2548, 3006
MmeI	TCCRAC	3 cut(s)	753, 1339, 3266
Mox20I	TGGCCA	1 cut(s)	543
Mph1103I	ATGCAT	1 cut(s)	3064
MroXI	GAANNNNTTC	1 cut(s)	2355
MscI	TGGCCA	1 cut(s)	543
MslI	CAYNNNNRTG	2 cut(s)	2376, 3018
Msp20I	TGGCCA	1 cut(s)	543
MspA1I	CMGCKG	3 cut(s)	809, 1961, 2090
MspI	CCGG	5 cut(s)	273, 387, 563, 601, 1699
MspR9I	CCNGG	3 cut(s)	660, 1256, 1833
MunI	CAATTG	1 cut(s)	2602
Mva1269I	GAATGC	1 cut(s)	1595
MvaI	CCWGG	3 cut(s)	660, 1256, 1833
MvnI	CGCG	3 cut(s)	508, 609, 3250
MwoI	GCNNNNNNNGC	9 cut(s)	89, 139, 184, 197, 794, 803, 1613, 1808, 3136
NcoI	CCATGG	1 cut(s)	544
NdeI	CATATG	1 cut(s)	3123
NlaIV	GGNNCC	3 cut(s)	1570, 2118, 3240
NmeAIII	GCCGAG	2 cut(s)	356, 1883
NmuCI	GTSAC	3 cut(s)	623, 3053, 3285
NsbI	TGCGCA	1 cut(s)	141
NsiI	ATGCAT	1 cut(s)	3064
NspI	RCATGY	2 cut(s)	562, 872
PaeI	GCATGC	1 cut(s)	562
PagI	TCATGA	1 cut(s)	2704
PceI	AGGCCT	1 cut(s)	2008
PciSI	GCTCTTC	2 cut(s)	99, 922
PcsI	WCGNNNNNNNCGW	2 cut(s)	411, 447
PctI	GAATGC	1 cut(s)	1595
PdmI	GAANNNNTTC	1 cut(s)	2355
PinAI	ACCGGT	1 cut(s)	272
PleI	GAGTC	7 cut(s)	33, 1015, 1090, 1449, 1868, 2548, 3005
PpsI	GAGTC	7 cut(s)	33, 1015, 1090, 1449, 1868, 2548, 3005
PpuMI	RGGWCCY	1 cut(s)	656
Psp124BI	GAGCTC	2 cut(s)	193, 293
Psp5II	RGGWCCY	1 cut(s)	656
Psp6I	CCWGG	3 cut(s)	658, 1254, 1831
PspFI	CCCAGC	1 cut(s)	797
PspGI	CCWGG	3 cut(s)	658, 1254, 1831
PspN4I	GGNNCC	3 cut(s)	1570, 2118, 3240
PspPI	GGNCC	1 cut(s)	656
PspPPI	RGGWCCY	1 cut(s)	656
PstI	CTGCAG	2 cut(s)	655, 1105
PstNI	CAGNNNCTG	3 cut(s)	260, 659, 1964
PsuI	RGATCY	3 cut(s)	1568, 1632, 2198
PvuII	CAGCTG	2 cut(s)	1961, 2090
RsaI	GTAC	5 cut(s)	358, 572, 2118, 2213, 3281
RsaNI	GTAC	5 cut(s)	357, 571, 2117, 2212, 3280
RseI	CAYNNNNRTG	2 cut(s)	2376, 3018
SacI	GAGCTC	2 cut(s)	193, 293
SapI	GCTCTTC	2 cut(s)	99, 922
Sau96I	GGNCC	1 cut(s)	656
ScaI	AGTACT	1 cut(s)	572
SchI	GAGTC	7 cut(s)	33, 1016, 1090, 1449, 1869, 2548, 3006
ScrFI	CCNGG	3 cut(s)	660, 1256, 1833
SduI	GDGCHC	5 cut(s)	193, 293, 1673, 2563, 3121
SfaNI	GCATC	9 cut(s)	127, 734, 1033, 1508, 1594, 2601, 2710, 3049, 3117
SfcI	CTRYAG	4 cut(s)	651, 753, 1101, 2820
SinI	GGWCC	1 cut(s)	656
SmiMI	CAYNNNNRTG	2 cut(s)	2376, 3018
SmlI	CTYRAG	3 cut(s)	292, 881, 2019
SmoI	CTYRAG	3 cut(s)	292, 881, 2019
SphI	GCATGC	1 cut(s)	562
SseBI	AGGCCT	1 cut(s)	2008
SspMI	CTAG	2 cut(s)	737, 2799
SstI	GAGCTC	2 cut(s)	193, 293
StuI	AGGCCT	1 cut(s)	2008
StyD4I	CCNGG	3 cut(s)	658, 1254, 1831
StyI	CCWWGG	3 cut(s)	87, 544, 1182
TaiI	ACGT	4 cut(s)	2992, 3084, 3118, 3248
TaqI	TCGA	9 cut(s)	99, 414, 450, 862, 1376, 2154, 2563, 2656, 2758
TaqII	GACCGA	1 cut(s)	1461
TatI	WGTACW	3 cut(s)	356, 570, 3279
TauI	GCSGC	4 cut(s)	86, 383, 812, 2261
TscAI	CASTG	5 cut(s)	1006, 1558, 1984, 2109, 3294
TseFI	GTSAC	3 cut(s)	623, 3053, 3285
Tsp45I	GTSAC	3 cut(s)	623, 3053, 3285
TspGWI	ACGGA	3 cut(s)	59, 1412, 2609
TspRI	CASTG	5 cut(s)	1006, 1558, 1984, 2109, 3294
VpaK11BI	GGWCC	1 cut(s)	656
XceI	RCATGY	2 cut(s)	562, 872
XmiI	GTMKAC	1 cut(s)	1475
XmnI	GAANNNNTTC	1 cut(s)	2355
XspI	CTAG	2 cut(s)	737, 2799
ZraI	GACGTC	1 cut(s)	3246
ZrmI	AGTACT	1 cut(s)	572
Zsp2I	ATGCAT	1 cut(s)	3064

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.