Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0001735

Physical Location & Seq

Genomic Coordinates Forward (+)

12798639 .. 12799628

Gene Structure 990 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1134500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 990 bp

ATGTTGGCAGCTAAAACCCTAATAAACCCATCGCCATCACTCAAGCTCCCAAACAACCTCTCCCTCCGCCGCAAACCCCCATTTTCTCTCTCGATCCGCAACTCTCTCTCCCAATCCCCGTCGTCTCCTGATCCCACTCCGGGGCAACTTCGAGTCGACATCCTCGCCGAGTCTCTCCCCTTCATCCAGAAATTCCGAGGCAAGACCATCGTCGTCAAGTATGGCGGCGCCGCCATGAAATCCGAGTCCCTCCAGGCCTCCGTCGTCAACGACCTCGTCCTCCTCTCCTGCGTCGGCCTCAGGCCTATCCTCGTCCACGGCGGCGGCCCCGAGATCAACCTCTTGCTCCGGCGCCTCGGCATCGAGGCGACCTTCGTCGACGGCCTCCGAGTCACCGACGCCAAAACAATGGAGGTCGTATCGATGGTCCTTGTAGGGAAAGTCAACAAAAATCTGGTCTCCTTAATCGACCGGGCCGGCGCCAAGGCAATCGGACTCTGCGGGATGGACGGGCGGCTTGTCACGGCCCGGCCCGTTCCGAACGCCGCCAAATTGGGATTTGTAGGGGAAGTTGCGAGGGTGGACCCCGCCGTGTTGCGGTCCAACGTCGAGGCAGGGTACATCCCGGTGATTGCGTCCGTGGCGGCGGACGAGACCGGGCAGCAGTATAACATCAATGCGGACACGGTGGCGGGGGAGCTGGCGGCGGCGGTGGGGGCGGAGAAGCTGATCCTGCTGACCGACGTGGCGGGGATTCTGGAGGATCGAAACGACGTGTCGAGTTTGGTAAAGGAGATTGATATCAATGGAGTGAAGAAGATGGTGGCGGAGGGGAAGATCGGCGGCGGAATGATTCCGAAGGTGAGTTGCTGCGTGAGGTCGCTGGCGCAGGGTGTGAAGACTGCTAGCATTGTTGATGGGAGAGTGCCCCATTCTCTGTTGCTTGAGATTTTGACTGATGAAGGGGCTGGAACTATGATTACTGGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003991 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006525 GO:0006526 GO:0006591 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009507 GO:0009534 GO:0009536 GO:0009579 GO:0009987 GO:0016053 GO:0016301 GO:0016310 GO:0016597 GO:0016740 GO:0016772 GO:0016774 GO:0019752 GO:0031406 GO:0031976 GO:0031984 GO:0034618 GO:0036094 GO:0042450 GO:0043167 GO:0043168 GO:0043169 GO:0043177 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00220 ko01100 ko01110 ko01130 ko01210 ko01230 map00220 map01100 map01110 map01130 map01210 map01230

Pfam Domains

Protein Families

AA_kinase

Protein Analysis

330

Amino Acids

34.4

Weight (kDa)

9.0

Isoelectric Point (pI)

40.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AA_kinase	PF00696	68 - 306	1.9e-45	Amino acid kinase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0001735)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G57560
fragaria_vesca	FvH4_2g30500
malus_domestica	MD08G1134500.v1.1
prunus_persica	Prupe.1G463900_v2.0.a1
pyrus_communis	pycom08g11350
rosa_chinensis	RchiOBHm_Chr3g0471221 RchiOBHm_Chr5g0021691 RchiOBHm_Chr6g0246551 RchiOBHm_Chr6g0292021 RchiOBHm_Chr6g0312541 RchiOBHm_Chr7g0238931
rosa_laevigata	RLG00000007795 RLG00000010124 RLG00000010318 RLG00000012398 RLG00000019113 RLG00000023496 RLG00000025257 RLG00000034574
rosa_multiflora	Rmu_sc0002754.1_g000042 Rmu_sc0003094.1_g000013 Rmu_sc0008090.1_g000001 Rmu_sc0008363.1_g000008
rosa_roxburghii	Rroxscaffold_7G00156700
rosa_rugosa	Rorug04G0331000 Rorug05G0155900 Rorug06G0396400 Rorug07G0284600
rosa_samantha	Rh1AG359700 Rh1BG130800 Rh3AG089900 Rh3BG190800 Rh3CG093300 Rh3CG181100 Rh5CG318300 Rh6BG526000 Rh6CG531100 Rh6DG516100 Rh7AG157800 Rh7AG157900 Rh7AG177800 Rh7AG188000 Rh7AG478800 Rh7BG405100 Rh7CG131900 Rh7DG455900
rosa_wichuraiana	Rw6G044660 Rw7G022170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	3 cut(s)	227, 351, 479
AccI	GTMKAC	2 cut(s)	156, 378
AclWI	GGATC	4 cut(s)	88, 125, 724, 771
AcsI	RAATTY	1 cut(s)	191
AcyI	GRCGYC	4 cut(s)	228, 352, 399, 480
AfaI	GTAC	1 cut(s)	620
AfiI	CCNNNNNNNGG	3 cut(s)	140, 141, 597
AflIII	ACRYGT	1 cut(s)	774
AjiI	CACGTC	2 cut(s)	745, 775
AjnI	CCWGG	1 cut(s)	252
AluBI	AGCT	4 cut(s)	11, 46, 700, 727
AluI	AGCT	4 cut(s)	11, 46, 700, 727
Alw26I	GTCTC	4 cut(s)	129, 177, 463, 647
AlwI	GGATC	4 cut(s)	88, 125, 724, 771
Ama87I	CYCGRG	1 cut(s)	329
AoxI	GGCC	8 cut(s)	255, 295, 302, 325, 382, 474, 525, 530
ApeKI	GCWGC	3 cut(s)	8, 661, 870
ApoI	RAATTY	1 cut(s)	191
ArsI	GACNNNNNNTTYG	2 cut(s)	441, 473
AspLEI	GCGC	4 cut(s)	230, 354, 482, 889
AspS9I	GGNCC	7 cut(s)	326, 427, 474, 526, 531, 583, 600
AsuC2I	CCSGG	5 cut(s)	141, 473, 529, 626, 658
AsuHPI	GGTGA	3 cut(s)	385, 640, 874
AsuNHI	GCTAGC	1 cut(s)	905
AvaI	CYCGRG	1 cut(s)	329
AvaII	GGWCC	3 cut(s)	427, 583, 600
AxyI	CCTNAGG	1 cut(s)	299
BaeGI	GKGCMC	1 cut(s)	930
BanI	GGYRCC	3 cut(s)	227, 351, 479
BbsI	GAAGAC	1 cut(s)	905
BbvI	GCAGC	3 cut(s)	20, 673, 857
BccI	CCATC	7 cut(s)	37, 43, 215, 418, 499, 814, 911
BceAI	ACGGC	4 cut(s)	334, 397, 540, 575
BcgI	CGANNNNNNTGC	2 cut(s)	190, 224
BciT130I	CCWGG	1 cut(s)	254
BcnI	CCSGG	5 cut(s)	141, 473, 529, 626, 658
BcoDI	GTCTC	4 cut(s)	129, 177, 463, 647
BfaI	CTAG	1 cut(s)	906
BfoI	RGCGCY	3 cut(s)	231, 355, 483
Bme1390I	CCNGG	6 cut(s)	141, 254, 473, 529, 626, 658
Bme18I	GGWCC	3 cut(s)	427, 583, 600
BmeT110I	CYCGRG	1 cut(s)	329
BmgBI	CACGTC	2 cut(s)	745, 775
BmgT120I	GGNCC	7 cut(s)	326, 427, 474, 526, 531, 583, 600
BmiI	GGNNCC	5 cut(s)	229, 328, 353, 481, 585
BmrFI	CCNGG	6 cut(s)	141, 254, 473, 529, 626, 658
BmsI	GCATC	1 cut(s)	369
BmtI	GCTAGC	1 cut(s)	909
BoxI	GACNNNNGTC	2 cut(s)	209, 374
BpiI	GAAGAC	1 cut(s)	905
BpmI	CTGGAG	2 cut(s)	236, 779
BpuEI	CTTGAG	2 cut(s)	26, 965
BpuMI	CCSGG	5 cut(s)	141, 473, 529, 626, 658
Bsa29I	ATCGAT	1 cut(s)	422
BsaBI	GATNNNNATC	1 cut(s)	800
BsaHI	GRCGYC	4 cut(s)	228, 352, 399, 480
BsaI	GGTCTC	2 cut(s)	463, 647
BsaJI	CCNNGG	6 cut(s)	140, 196, 316, 355, 483, 639
BsaXI	ACNNNNNCTCC	6 cut(s)	30, 44, 60, 74, 92, 122
Bsc4I	CCNNNNNNNGG	3 cut(s)	140, 141, 597
Bse118I	RCCGGY	1 cut(s)	476
Bse1I	ACTGG	1 cut(s)	988
Bse21I	CCTNAGG	1 cut(s)	299
Bse8I	GATNNNNATC	1 cut(s)	800
BseBI	CCWGG	1 cut(s)	254
BseCI	ATCGAT	1 cut(s)	422
BseDI	CCNNGG	6 cut(s)	140, 196, 316, 355, 483, 639
BseGI	GGATG	4 cut(s)	159, 183, 510, 621
BseJI	GATNNNNATC	1 cut(s)	800
BseLI	CCNNNNNNNGG	3 cut(s)	140, 141, 597
BseMII	CTCAG	1 cut(s)	313
BseNI	ACTGG	1 cut(s)	988
BseRI	GAGGAG	1 cut(s)	272
BseSI	GKGCMC	1 cut(s)	930
BseXI	GCAGC	3 cut(s)	20, 673, 857
Bsh1285I	CGRYCG	1 cut(s)	472
BshFI	GGCC	8 cut(s)	257, 297, 304, 327, 384, 476, 527, 532
BshNI	GGYRCC	3 cut(s)	227, 351, 479
BshVI	ATCGAT	1 cut(s)	422
BsiEI	CGRYCG	1 cut(s)	472
BsiHKCI	CYCGRG	1 cut(s)	329
BsiSI	CCGG	7 cut(s)	140, 349, 472, 477, 529, 626, 657
BslFI	GGGAC	1 cut(s)	232
BslI	CCNNNNNNNGG	3 cut(s)	140, 141, 597
BsmAI	GTCTC	4 cut(s)	129, 177, 463, 647
BsmBI	CGTCTC	1 cut(s)	129
BsmFI	GGGAC	1 cut(s)	232
BsnI	GGCC	8 cut(s)	257, 297, 304, 327, 384, 476, 527, 532
Bso31I	GGTCTC	2 cut(s)	463, 647
BsoBI	CYCGRG	1 cut(s)	329
Bsp1286I	GDGCHC	1 cut(s)	930
Bsp143I	GATC	6 cut(s)	93, 130, 333, 729, 763, 837
BspANI	GGCC	8 cut(s)	257, 297, 304, 327, 384, 476, 527, 532
BspCNI	CTCAG	1 cut(s)	312
BspDI	ATCGAT	1 cut(s)	422
BspLI	GGNNCC	5 cut(s)	229, 328, 353, 481, 585
BspOI	GCTAGC	1 cut(s)	909
BspPI	GGATC	4 cut(s)	88, 125, 724, 771
BspT107I	GGYRCC	3 cut(s)	227, 351, 479
BspTNI	GGTCTC	2 cut(s)	463, 647
BsrFI	RCCGGY	1 cut(s)	476
BsrI	ACTGG	1 cut(s)	988
BssAI	RCCGGY	1 cut(s)	476
BssECI	CCNNGG	6 cut(s)	140, 196, 316, 355, 483, 639
BssMI	GATC	6 cut(s)	93, 130, 333, 729, 763, 837
BssNI	GRCGYC	4 cut(s)	228, 352, 399, 480
BssT1I	CCWWGG	1 cut(s)	483
Bst2UI	CCWGG	1 cut(s)	254
Bst4CI	ACNGT	1 cut(s)	688
BstACI	GRCGYC	4 cut(s)	228, 352, 399, 480
BstC8I	GCNNGC	4 cut(s)	478, 702, 885, 907
BstDEI	CTNAG	1 cut(s)	299
BstDSI	CCRYGG	2 cut(s)	316, 639
BstF5I	GGATG	4 cut(s)	159, 183, 510, 621
BstH2I	RGCGCY	3 cut(s)	231, 355, 483
BstHHI	GCGC	4 cut(s)	230, 354, 482, 889
BstKTI	GATC	6 cut(s)	96, 133, 336, 732, 766, 840
BstMAI	GTCTC	4 cut(s)	129, 177, 463, 647
BstMBI	GATC	6 cut(s)	93, 130, 333, 729, 763, 837
BstMCI	CGRYCG	1 cut(s)	472
BstMWI	GCNNNNNNNGC	3 cut(s)	641, 716, 733
BstNI	CCWGG	1 cut(s)	254
BstPAI	GACNNNNGTC	2 cut(s)	209, 374
BstSCI	CCNGG	6 cut(s)	139, 252, 471, 527, 624, 656
BstSLI	GKGCMC	1 cut(s)	930
BstV1I	GCAGC	3 cut(s)	20, 673, 857
BstV2I	GAAGAC	1 cut(s)	905
BstXI	CCANNNNNNTGG	1 cut(s)	409
Bsu15I	ATCGAT	1 cut(s)	422
Bsu36I	CCTNAGG	1 cut(s)	299
BsuRI	GGCC	8 cut(s)	257, 297, 304, 327, 384, 476, 527, 532
BsuTUI	ATCGAT	1 cut(s)	422
BtgI	CCRYGG	2 cut(s)	316, 639
BtgZI	GCGATG	1 cut(s)	15
BtrI	CACGTC	2 cut(s)	745, 775
BtsCI	GGATG	4 cut(s)	159, 183, 510, 621
Cac8I	GCNNGC	4 cut(s)	478, 702, 885, 907
CfoI	GCGC	4 cut(s)	230, 354, 482, 889
Cfr10I	RCCGGY	1 cut(s)	476
Cfr13I	GGNCC	7 cut(s)	326, 427, 474, 526, 531, 583, 600
ClaI	ATCGAT	1 cut(s)	422
CseI	GACGC	3 cut(s)	280, 407, 624
Csp6I	GTAC	1 cut(s)	619
CviAII	CATG	1 cut(s)	235
CviQI	GTAC	1 cut(s)	619
DdeI	CTNAG	1 cut(s)	299
DinI	GGCGCC	3 cut(s)	229, 353, 481
DpnI	GATC	6 cut(s)	95, 132, 335, 731, 765, 839
DpnII	GATC	6 cut(s)	93, 130, 333, 729, 763, 837
EciI	GGCGGA	5 cut(s)	56, 662, 734, 842, 861
Eco130I	CCWWGG	1 cut(s)	483
Eco147I	AGGCCT	2 cut(s)	257, 304
Eco31I	GGTCTC	2 cut(s)	463, 647
Eco32I	GATATC	1 cut(s)	802
Eco47I	GGWCC	3 cut(s)	427, 583, 600
Eco81I	CCTNAGG	1 cut(s)	299
Eco88I	CYCGRG	1 cut(s)	329
EcoRII	CCWGG	1 cut(s)	252
EcoRV	GATATC	1 cut(s)	802
EcoT14I	CCWWGG	1 cut(s)	483
EgeI	GGCGCC	3 cut(s)	229, 353, 481
EheI	GGCGCC	3 cut(s)	229, 353, 481
ErhI	CCWWGG	1 cut(s)	483
Esp3I	CGTCTC	1 cut(s)	129
FaeI	CATG	1 cut(s)	238
FaiI	YATR	4 cut(s)	222, 236, 669, 977
FaqI	GGGAC	1 cut(s)	232
FatI	CATG	1 cut(s)	234
FauI	CCCGC	4 cut(s)	494, 595, 685, 742
FblI	GTMKAC	2 cut(s)	156, 378
FokI	GGATG	4 cut(s)	146, 170, 517, 608
FspBI	CTAG	1 cut(s)	906
GlaI	GCGC	4 cut(s)	229, 353, 481, 888
GsuI	CTGGAG	2 cut(s)	236, 779
HaeII	RGCGCY	3 cut(s)	231, 355, 483
HaeIII	GGCC	8 cut(s)	257, 297, 304, 327, 384, 476, 527, 532
HapII	CCGG	7 cut(s)	140, 349, 472, 477, 529, 626, 657
HgaI	GACGC	3 cut(s)	280, 407, 624
HhaI	GCGC	4 cut(s)	230, 354, 482, 889
Hin1I	GRCGYC	4 cut(s)	228, 352, 399, 480
Hin1II	CATG	1 cut(s)	238
Hin6I	GCGC	4 cut(s)	228, 352, 480, 887
HinP1I	GCGC	4 cut(s)	228, 352, 480, 887
HincII	GTYRAC	4 cut(s)	157, 268, 379, 445
HindII	GTYRAC	4 cut(s)	157, 268, 379, 445
HinfI	GANTC	7 cut(s)	153, 170, 245, 390, 495, 754, 853
HpaII	CCGG	7 cut(s)	140, 349, 472, 477, 529, 626, 657
HphI	GGTGA	3 cut(s)	385, 640, 874
Hpy166II	GTNNAC	6 cut(s)	157, 268, 316, 379, 445, 583
Hpy188I	TCNGA	6 cut(s)	197, 244, 389, 494, 540, 858
Hpy188III	TCNNGA	4 cut(s)	91, 128, 187, 758
Hpy8I	GTNNAC	6 cut(s)	157, 268, 316, 379, 445, 583
HpyAV	CCTTC	4 cut(s)	190, 382, 853, 956
HpyCH4III	ACNGT	1 cut(s)	688
HpyCH4IV	ACGT	3 cut(s)	606, 744, 774
HpyF10VI	GCNNNNNNNGC	3 cut(s)	641, 716, 733
HpyF3I	CTNAG	1 cut(s)	299
HpySE526I	ACGT	3 cut(s)	606, 744, 774
Hsp92I	GRCGYC	4 cut(s)	228, 352, 399, 480
Hsp92II	CATG	1 cut(s)	238
HspAI	GCGC	4 cut(s)	228, 352, 480, 887
KasI	GGCGCC	3 cut(s)	227, 351, 479
KroI	GCCGGC	1 cut(s)	476
KroNI	GCCGGC	1 cut(s)	478
Kzo9I	GATC	6 cut(s)	93, 130, 333, 729, 763, 837
LmnI	GCTCC	3 cut(s)	51, 351, 697
Lsp1109I	GCAGC	3 cut(s)	20, 673, 857
LweI	GCATC	1 cut(s)	369
MaeI	CTAG	1 cut(s)	906
MaeII	ACGT	3 cut(s)	606, 744, 774
MaeIII	GTNAC	2 cut(s)	391, 520
MalI	GATC	6 cut(s)	95, 132, 335, 731, 765, 839
MboI	GATC	6 cut(s)	93, 130, 333, 729, 763, 837
MboII	GAAGA	4 cut(s)	826, 829, 847, 910
MhlI	GDGCHC	1 cut(s)	930
MluCI	AATT	2 cut(s)	191, 551
Mly113I	GGCGCC	3 cut(s)	228, 352, 480
MlyI	GAGTC	5 cut(s)	162, 179, 254, 399, 489
MmeI	TCCRAC	1 cut(s)	627
MroNI	GCCGGC	1 cut(s)	476
MseI	TTAA	1 cut(s)	464
MslI	CAYNNNNRTG	1 cut(s)	626
MspI	CCGG	7 cut(s)	140, 349, 472, 477, 529, 626, 657
MspR9I	CCNGG	6 cut(s)	141, 254, 473, 529, 626, 658
MvaI	CCWGG	1 cut(s)	254
MwoI	GCNNNNNNNGC	3 cut(s)	641, 716, 733
NaeI	GCCGGC	1 cut(s)	478
NarI	GGCGCC	3 cut(s)	228, 352, 480
NciI	CCSGG	5 cut(s)	141, 473, 529, 626, 658
NdeII	GATC	6 cut(s)	93, 130, 333, 729, 763, 837
NgoMIV	GCCGGC	1 cut(s)	476
NheI	GCTAGC	1 cut(s)	905
NlaIII	CATG	1 cut(s)	238
NlaIV	GGNNCC	5 cut(s)	229, 328, 353, 481, 585
NmeAIII	GCCGAG	2 cut(s)	193, 336
NmuCI	GTSAC	2 cut(s)	391, 520
PceI	AGGCCT	2 cut(s)	257, 304
PdiI	GCCGGC	1 cut(s)	478
PfeI	GAWTC	2 cut(s)	754, 853
PflFI	GACNNNGTC	1 cut(s)	275
PleI	GAGTC	5 cut(s)	161, 178, 253, 398, 489
PluTI	GGCGCC	3 cut(s)	231, 355, 483
PpsI	GAGTC	5 cut(s)	161, 178, 253, 398, 489
PshAI	GACNNNNGTC	2 cut(s)	209, 374
Psp6I	CCWGG	1 cut(s)	252
PspGI	CCWGG	1 cut(s)	252
PspN4I	GGNNCC	5 cut(s)	229, 328, 353, 481, 585
PspPI	GGNCC	7 cut(s)	326, 427, 474, 526, 531, 583, 600
PsrI	GAACNNNNNNTAC	1 cut(s)	964
PsyI	GACNNNGTC	1 cut(s)	275
RsaI	GTAC	1 cut(s)	620
RsaNI	GTAC	1 cut(s)	619
RseI	CAYNNNNRTG	1 cut(s)	626
SalI	GTCGAC	2 cut(s)	155, 377
SaqAI	TTAA	1 cut(s)	464
Sau3AI	GATC	6 cut(s)	93, 130, 333, 729, 763, 837
Sau96I	GGNCC	7 cut(s)	326, 427, 474, 526, 531, 583, 600
SchI	GAGTC	5 cut(s)	162, 179, 254, 399, 489
ScrFI	CCNGG	6 cut(s)	141, 254, 473, 529, 626, 658
SduI	GDGCHC	1 cut(s)	930
SfaNI	GCATC	1 cut(s)	369
SfoI	GGCGCC	3 cut(s)	229, 353, 481
SgrDI	CGTCGACG	1 cut(s)	377
SinI	GGWCC	3 cut(s)	427, 583, 600
SmiMI	CAYNNNNRTG	1 cut(s)	626
SmlI	CTYRAG	2 cut(s)	41, 944
SmoI	CTYRAG	2 cut(s)	41, 944
Sse9I	AATT	2 cut(s)	191, 551
SseBI	AGGCCT	2 cut(s)	257, 304
SspDI	GGCGCC	3 cut(s)	227, 351, 479
SspMI	CTAG	1 cut(s)	906
StuI	AGGCCT	2 cut(s)	257, 304
StyD4I	CCNGG	6 cut(s)	139, 252, 471, 527, 624, 656
StyI	CCWWGG	1 cut(s)	483
TaaI	ACNGT	1 cut(s)	688
TaiI	ACGT	3 cut(s)	609, 747, 777
TaqII	GACCGA	1 cut(s)	755
TasI	AATT	2 cut(s)	191, 551
TfiI	GAWTC	2 cut(s)	754, 853
Tru1I	TTAA	1 cut(s)	464
Tru9I	TTAA	1 cut(s)	464
TseFI	GTSAC	2 cut(s)	391, 520
TseI	GCWGC	3 cut(s)	8, 661, 870
Tsp45I	GTSAC	2 cut(s)	391, 520
TspDTI	ATGAA	3 cut(s)	172, 251, 975
TspGWI	ACGGA	2 cut(s)	250, 628
Tth111I	GACNNNGTC	1 cut(s)	275
VpaK11BI	GGWCC	3 cut(s)	427, 583, 600
XapI	RAATTY	1 cut(s)	191
XmiI	GTMKAC	2 cut(s)	156, 378
XspI	CTAG	1 cut(s)	906

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.