MD09G1022800.v1.1

Protein of unknown function (DUF707)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0001946
Physical Location & Seq
Forward (+)
1363824 .. 1367298
3475 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1022800.v1.1.491

Sequence Viewer

Length: 1209 bp
ATGGGGTCTCCGATACGCATCGGAAATTTAAGAAACCCCAATGAGACAATGAGGCTTGCAGTGACAATCTTTGTTGGAGTTGTAGTCGGGTTCTTTTTAGGAGTATCGTTTTCAACAATTTCGTTGACTAAGATGAATCTTCCGTCCACCCTTTTTCCTTCAATTGATCTTTCGTGCATAGAGGAAAAGTACTCCGGGTTTTCAACCCAAGCAATGTTGAGTGCCTGGTCTTCTTTGAAGGGAAACGAGCAGCGCACTGAAAAATATGTATCTCAGGATACAAAGATTTGGGTTCCAACTAATCCTCGAGGGGCTGAAAGCCTAGCGCCTGGTATCATTGCATCTGAGTCGGATTTCTATCTTCACCGACTATGGGGTCTGCCTAGTGAGGACTTACTTGTCAAGCCAAAGTATCTTGTAACCTTTACCGTAGGTTGTGCTCAGAAAAAAAACGTCGATGCAGCAGTTAAAAAGTTTTCAGAGAATTTCACCATACTTTTATTTCACTACGATGGTTTGACAAGTGAATGGGATGAGTTTGAATGGTCGAAGCGAGCTATCCACATGAGCATTCAGAAGCAAACTAAATGGTGGTACGCCAAGCGGTTTTTGCATCCTGACATTGTGGCACCGTACGAATACATATTTATTTGGGATGAGGATCTTGGTCTGGAACACTTTGATGCAGAGAAATACATAAAATTGGTAAAGAAACATGGTTTGGAAATATCACAGCCAGGTTTAGATCCAAAGAGTGGGCTAACATGGCAGATGACGAGGCGGAGAGAAAACAGCGAAGTTCACATGCAGACAGAGGAGAGACCTGGTTGGTGCAGTGATCCGCATTTGCCACCATGTGCATCGTTTGTTGAAATTATGGCACCTGTGTTCTCTCGGGATGCATGGCGTTGTGTCTGGCATATGATTCAGAATGACCTGGTCCATGGCTGGGGTCTGGATTTTGCTCTTAGAAATTGCGTTGAGCCTGCACATAAGAAAATAGGAGTCGTGGACGCGCAATGGATTGTCCATCAAGGCGTTCCTTCACTCGGGAACCAAGGGCGACCTGAGGGTGGAAGAGCACCATGGGAAGGGGTAAGGGAGAGGTGTAATAGCGAATGGGCAATGTTTCAAGCACGAATGACCGGCGCCGAGAAAGCATACTTTGAGGAAATGGGAATTGATCCTCCAAATTCAACAGCTCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF707

Protein Analysis

403

Amino Acids

45.89

Weight (kDa)

6.33

Isoelectric Point (pI)

38.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF707 PF05212 100 - 386 2.4e-138 Protein of unknown function (DUF707)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 375, 398
AbsI CCTCGAGG 1 cut(s) 306
AccB1I GGYRCC 3 cut(s) 628, 880, 1148
AccB7I CCANNNNNTGG 1 cut(s) 755
AccII CGCG 1 cut(s) 1016
AciI CCGC 3 cut(s) 604, 781, 842
AclWI GGATC 4 cut(s) 669, 740, 833, 1178
AcsI RAATTY 3 cut(s) 25, 484, 1192
AcyI GRCGYC 1 cut(s) 1149
AdeI CACNNNGTG 1 cut(s) 857
AfaI GTAC 3 cut(s) 191, 596, 635
AfiI CCNNNNNNNGG 6 cut(s) 373, 755, 949, 1049, 1073, 1091
AgsI TTSAA 8 cut(s) 114, 162, 204, 238, 542, 872, 1133, 1197
AjnI CCWGG 5 cut(s) 224, 328, 736, 823, 936
AjuI GAANNNNNNNTTGG 2 cut(s) 704, 736
AluBI AGCT 2 cut(s) 557, 1202
AluI AGCT 2 cut(s) 557, 1202
Alw21I GWGCWC 2 cut(s) 442, 1084
Alw26I GTCTC 3 cut(s) 12, 38, 814
AlwI GGATC 4 cut(s) 669, 740, 833, 1178
Ama87I CYCGRG 3 cut(s) 306, 894, 1049
ApeKI GCWGC 2 cut(s) 250, 461
ApoI RAATTY 3 cut(s) 25, 484, 1192
AspLEI GCGC 4 cut(s) 255, 328, 1018, 1151
AspS9I GGNCC 1 cut(s) 940
AsuC2I CCSGG 1 cut(s) 196
AsuHPI GGTGA 2 cut(s) 356, 481
AvaI CYCGRG 3 cut(s) 306, 894, 1049
AvaII GGWCC 1 cut(s) 940
AxyI CCTNAGG 1 cut(s) 1068
BanI GGYRCC 3 cut(s) 628, 880, 1148
BbsI GAAGAC 1 cut(s) 222
Bbv12I GWGCWC 2 cut(s) 442, 1084
BbvI GCAGC 2 cut(s) 262, 473
BccI CCATC 2 cut(s) 506, 1038
BcgI CGANNNNNNTGC 2 cut(s) 330, 364
BciT130I CCWGG 5 cut(s) 226, 330, 738, 825, 938
BciVI GTATCC 1 cut(s) 271
BcnI CCSGG 1 cut(s) 196
BcoDI GTCTC 3 cut(s) 12, 38, 814
BfaI CTAG 2 cut(s) 323, 384
BfoI RGCGCY 2 cut(s) 329, 1152
BfuI GTATCC 1 cut(s) 271
BisI GCNGC 2 cut(s) 251, 462
BlsI GCNGC 2 cut(s) 252, 463
BmcAI AGTACT 1 cut(s) 191
Bme1390I CCNGG 6 cut(s) 196, 226, 330, 738, 825, 938
Bme18I GGWCC 1 cut(s) 940
BmeT110I CYCGRG 3 cut(s) 306, 894, 1049
BmgT120I GGNCC 1 cut(s) 940
BmiI GGNNCC 5 cut(s) 294, 630, 882, 1055, 1150
BmrFI CCNGG 6 cut(s) 196, 226, 330, 738, 825, 938
BmsI GCATC 7 cut(s) 27, 350, 448, 622, 673, 869, 889
BpiI GAAGAC 1 cut(s) 222
BpuMI CCSGG 1 cut(s) 196
BsaBI GATNNNNATC 3 cut(s) 17, 357, 660
BsaHI GRCGYC 1 cut(s) 1149
BsaI GGTCTC 2 cut(s) 12, 814
BsaJI CCNNGG 3 cut(s) 943, 1057, 1085
BsaXI ACNNNNNCTCC 2 cut(s) 69, 99
Bsc4I CCNNNNNNNGG 6 cut(s) 373, 755, 949, 1049, 1073, 1091
Bse118I RCCGGY 1 cut(s) 1145
Bse21I CCTNAGG 1 cut(s) 1068
Bse3DI GCAATG 4 cut(s) 219, 336, 1025, 1131
Bse8I GATNNNNATC 3 cut(s) 17, 357, 660
BseBI CCWGG 5 cut(s) 226, 330, 738, 825, 938
BseDI CCNNGG 3 cut(s) 943, 1057, 1085
BseGI GGATG 4 cut(s) 538, 613, 661, 904
BseJI GATNNNNATC 3 cut(s) 17, 357, 660
BseLI CCNNNNNNNGG 6 cut(s) 373, 755, 949, 1049, 1073, 1091
BseMI GCAATG 4 cut(s) 219, 336, 1025, 1131
BseMII CTCAG 4 cut(s) 287, 336, 455, 1059
BseRI GAGGAG 1 cut(s) 830
BseXI GCAGC 2 cut(s) 262, 473
BseYI CCCAGC 1 cut(s) 948
BsgI GTGCAG 2 cut(s) 853, 972
Bsh1236I CGCG 1 cut(s) 1016
BshNI GGYRCC 3 cut(s) 628, 880, 1148
BsiHKAI GWGCWC 2 cut(s) 442, 1084
BsiHKCI CYCGRG 3 cut(s) 306, 894, 1049
BsiSI CCGG 2 cut(s) 195, 1146
BsiWI CGTACG 1 cut(s) 633
BslI CCNNNNNNNGG 6 cut(s) 373, 755, 949, 1049, 1073, 1091
BsmAI GTCTC 3 cut(s) 12, 38, 814
BsmI GAATGC 1 cut(s) 570
Bso31I GGTCTC 2 cut(s) 12, 814
BsoBI CYCGRG 3 cut(s) 306, 894, 1049
Bsp1286I GDGCHC 2 cut(s) 442, 1084
Bsp143I GATC 5 cut(s) 166, 661, 745, 838, 1183
Bsp19I CCATGG 2 cut(s) 943, 1085
BspACI CCGC 3 cut(s) 604, 781, 842
BspCNI CTCAG 4 cut(s) 286, 337, 454, 1060
BspFNI CGCG 1 cut(s) 1016
BspLI GGNNCC 5 cut(s) 294, 630, 882, 1055, 1150
BspPI GGATC 4 cut(s) 669, 740, 833, 1178
BspQI GCTCTTC 1 cut(s) 1072
BspT107I GGYRCC 3 cut(s) 628, 880, 1148
BspTNI GGTCTC 2 cut(s) 12, 814
BsrDI GCAATG 4 cut(s) 219, 336, 1025, 1131
BsrFI RCCGGY 1 cut(s) 1145
BssAI RCCGGY 1 cut(s) 1145
BssECI CCNNGG 3 cut(s) 943, 1057, 1085
BssMI GATC 5 cut(s) 166, 661, 745, 838, 1183
BssNI GRCGYC 1 cut(s) 1149
BssT1I CCWWGG 3 cut(s) 943, 1057, 1085
Bst2UI CCWGG 5 cut(s) 226, 330, 738, 825, 938
Bst4CI ACNGT 2 cut(s) 430, 633
Bst6I CTCTTC 1 cut(s) 1072
BstACI GRCGYC 1 cut(s) 1149
BstC8I GCNNGC 3 cut(s) 57, 555, 987
BstDEI CTNAG 6 cut(s) 129, 273, 345, 441, 968, 1068
BstDSI CCRYGG 2 cut(s) 943, 1085
BstF5I GGATG 4 cut(s) 538, 613, 661, 904
BstFNI CGCG 1 cut(s) 1016
BstH2I RGCGCY 2 cut(s) 329, 1152
BstHHI GCGC 4 cut(s) 255, 328, 1018, 1151
BstKTI GATC 5 cut(s) 169, 664, 748, 841, 1186
BstMAI GTCTC 3 cut(s) 12, 38, 814
BstMBI GATC 5 cut(s) 166, 661, 745, 838, 1183
BstMWI GCNNNNNNNGC 3 cut(s) 610, 766, 1157
BstNI CCWGG 5 cut(s) 226, 330, 738, 825, 938
BstNSI RCATGY 1 cut(s) 808
BstSCI CCNGG 6 cut(s) 194, 224, 328, 736, 823, 936
BstUI CGCG 1 cut(s) 1016
BstV1I GCAGC 2 cut(s) 262, 473
BstV2I GAAGAC 1 cut(s) 222
BstX2I RGATCY 2 cut(s) 661, 745
BstYI RGATCY 2 cut(s) 661, 745
Bsu36I CCTNAGG 1 cut(s) 1068
BsuI GTATCC 1 cut(s) 271
BtgI CCRYGG 2 cut(s) 943, 1085
BtsCI GGATG 4 cut(s) 538, 613, 661, 904
BtsI GCAGTG 2 cut(s) 66, 841
BtsIMutI CAGTG 3 cut(s) 66, 255, 841
Cac8I GCNNGC 3 cut(s) 57, 555, 987
CfoI GCGC 4 cut(s) 255, 328, 1018, 1151
Cfr10I RCCGGY 1 cut(s) 1145
Cfr13I GGNCC 1 cut(s) 940
CseI GACGC 1 cut(s) 1022
CsiI ACCWGGT 2 cut(s) 823, 936
Csp6I GTAC 3 cut(s) 190, 595, 634
CviAII CATG 8 cut(s) 565, 716, 765, 805, 855, 903, 944, 1086
CviQI GTAC 3 cut(s) 190, 595, 634
DdeI CTNAG 6 cut(s) 129, 273, 345, 441, 968, 1068
DinI GGCGCC 1 cut(s) 1150
DpnI GATC 5 cut(s) 168, 663, 747, 840, 1185
DpnII GATC 5 cut(s) 166, 661, 745, 838, 1183
DraIII CACNNNGTG 1 cut(s) 857
DrdI GACNNNNNNGTC 2 cut(s) 375, 398
DseDI GACNNNNNNGTC 2 cut(s) 375, 398
Eam1104I CTCTTC 1 cut(s) 1072
EarI CTCTTC 1 cut(s) 1072
EciI GGCGGA 1 cut(s) 796
Eco130I CCWWGG 3 cut(s) 943, 1057, 1085
Eco31I GGTCTC 2 cut(s) 12, 814
Eco47I GGWCC 1 cut(s) 940
Eco81I CCTNAGG 1 cut(s) 1068
Eco88I CYCGRG 3 cut(s) 306, 894, 1049
EcoRII CCWGG 5 cut(s) 224, 328, 736, 823, 936
EcoT14I CCWWGG 3 cut(s) 943, 1057, 1085
EcoT22I ATGCAT 1 cut(s) 904
EgeI GGCGCC 1 cut(s) 1150
EheI GGCGCC 1 cut(s) 1150
ErhI CCWWGG 3 cut(s) 943, 1057, 1085
FaeI CATG 8 cut(s) 568, 719, 768, 808, 858, 906, 947, 1089
FatI CATG 8 cut(s) 564, 715, 764, 804, 854, 902, 943, 1085
FauNDI CATATG 1 cut(s) 921
Fnu4HI GCNGC 2 cut(s) 251, 462
FokI GGATG 4 cut(s) 545, 600, 668, 911
Fsp4HI GCNGC 2 cut(s) 251, 462
FspBI CTAG 2 cut(s) 323, 384
GlaI GCGC 4 cut(s) 254, 327, 1017, 1150
GluI GCNGC 2 cut(s) 251, 462
GsaI CCCAGC 1 cut(s) 952
HaeII RGCGCY 2 cut(s) 329, 1152
HapII CCGG 2 cut(s) 195, 1146
HgaI GACGC 1 cut(s) 1022
HhaI GCGC 4 cut(s) 255, 328, 1018, 1151
Hin1I GRCGYC 1 cut(s) 1149
Hin1II CATG 8 cut(s) 568, 719, 768, 808, 858, 906, 947, 1089
Hin6I GCGC 4 cut(s) 253, 326, 1016, 1149
HinP1I GCGC 4 cut(s) 253, 326, 1016, 1149
HincII GTYRAC 1 cut(s) 126
HindII GTYRAC 1 cut(s) 126
HinfI GANTC 4 cut(s) 136, 347, 925, 1005
HpaII CCGG 2 cut(s) 195, 1146
HphI GGTGA 2 cut(s) 356, 481
Hpy166II GTNNAC 4 cut(s) 126, 147, 802, 1012
Hpy188I TCNGA 8 cut(s) 12, 23, 346, 352, 444, 481, 576, 930
Hpy188III TCNNGA 6 cut(s) 275, 617, 671, 896, 956, 1051
Hpy8I GTNNAC 4 cut(s) 126, 147, 802, 1012
Hpy99I CGWCG 1 cut(s) 458
HpyAV CCTTC 4 cut(s) 168, 232, 1053, 1085
HpyCH4III ACNGT 2 cut(s) 430, 633
HpyCH4IV ACGT 1 cut(s) 453
HpyF10VI GCNNNNNNNGC 3 cut(s) 610, 766, 1157
HpyF3I CTNAG 6 cut(s) 129, 273, 345, 441, 968, 1068
HpySE526I ACGT 1 cut(s) 453
Hsp92I GRCGYC 1 cut(s) 1149
Hsp92II CATG 8 cut(s) 568, 719, 768, 808, 858, 906, 947, 1089
HspAI GCGC 4 cut(s) 253, 326, 1016, 1149
KasI GGCGCC 1 cut(s) 1148
Kzo9I GATC 5 cut(s) 166, 661, 745, 838, 1183
LguI GCTCTTC 1 cut(s) 1072
Lsp1109I GCAGC 2 cut(s) 262, 473
LweI GCATC 7 cut(s) 27, 350, 448, 622, 673, 869, 889
MabI ACCWGGT 2 cut(s) 823, 936
MaeI CTAG 2 cut(s) 323, 384
MaeII ACGT 1 cut(s) 453
MaeIII GTNAC 2 cut(s) 61, 418
MalI GATC 5 cut(s) 168, 663, 747, 840, 1185
MboI GATC 5 cut(s) 166, 661, 745, 838, 1183
MboII GAAGA 4 cut(s) 131, 222, 353, 1089
MfeI CAATTG 1 cut(s) 162
MflI RGATCY 2 cut(s) 661, 745
MhlI GDGCHC 2 cut(s) 442, 1084
MluCI AATT 9 cut(s) 25, 117, 162, 484, 701, 873, 973, 1179, 1192
Mly113I GGCGCC 1 cut(s) 1149
MlyI GAGTC 2 cut(s) 356, 1014
MmeI TCCRAC 3 cut(s) 55, 320, 330
Mph1103I ATGCAT 1 cut(s) 904
MseI TTAA 2 cut(s) 29, 468
MslI CAYNNNNRTG 2 cut(s) 510, 681
MspI CCGG 2 cut(s) 195, 1146
MspR9I CCNGG 6 cut(s) 196, 226, 330, 738, 825, 938
MunI CAATTG 1 cut(s) 162
Mva1269I GAATGC 1 cut(s) 570
MvaI CCWGG 5 cut(s) 226, 330, 738, 825, 938
MvnI CGCG 1 cut(s) 1016
MwoI GCNNNNNNNGC 3 cut(s) 610, 766, 1157
NarI GGCGCC 1 cut(s) 1149
NciI CCSGG 1 cut(s) 196
NcoI CCATGG 2 cut(s) 943, 1085
NdeI CATATG 1 cut(s) 921
NdeII GATC 5 cut(s) 166, 661, 745, 838, 1183
NlaIII CATG 8 cut(s) 568, 719, 768, 808, 858, 906, 947, 1089
NlaIV GGNNCC 5 cut(s) 294, 630, 882, 1055, 1150
NmeAIII GCCGAG 1 cut(s) 1177
NmuCI GTSAC 1 cut(s) 61
NsiI ATGCAT 1 cut(s) 904
NspI RCATGY 1 cut(s) 808
PaeR7I CTCGAG 1 cut(s) 306
PciSI GCTCTTC 1 cut(s) 1072
PctI GAATGC 1 cut(s) 570
PfeI GAWTC 2 cut(s) 136, 925
Pfl23II CGTACG 1 cut(s) 633
PflFI GACNNNGTC 1 cut(s) 938
PflMI CCANNNNNTGG 1 cut(s) 755
PkrI GCNGC 2 cut(s) 252, 463
PleI GAGTC 2 cut(s) 355, 1013
PluTI GGCGCC 1 cut(s) 1152
PpsI GAGTC 2 cut(s) 355, 1013
Psp6I CCWGG 5 cut(s) 224, 328, 736, 823, 936
PspFI CCCAGC 1 cut(s) 948
PspGI CCWGG 5 cut(s) 224, 328, 736, 823, 936
PspLI CGTACG 1 cut(s) 633
PspN4I GGNNCC 5 cut(s) 294, 630, 882, 1055, 1150
PspPI GGNCC 1 cut(s) 940
PspXI VCTCGAGB 1 cut(s) 306
PsuI RGATCY 2 cut(s) 661, 745
PsyI GACNNNGTC 1 cut(s) 938
RsaI GTAC 3 cut(s) 191, 596, 635
RsaNI GTAC 3 cut(s) 190, 595, 634
RseI CAYNNNNRTG 2 cut(s) 510, 681
SapI GCTCTTC 1 cut(s) 1072
SaqAI TTAA 2 cut(s) 29, 468
SatI GCNGC 2 cut(s) 251, 462
Sau3AI GATC 5 cut(s) 166, 661, 745, 838, 1183
Sau96I GGNCC 1 cut(s) 940
ScaI AGTACT 1 cut(s) 191
SchI GAGTC 2 cut(s) 356, 1014
ScrFI CCNGG 6 cut(s) 196, 226, 330, 738, 825, 938
SduI GDGCHC 2 cut(s) 442, 1084
SexAI ACCWGGT 2 cut(s) 823, 936
SfaNI GCATC 7 cut(s) 27, 350, 448, 622, 673, 869, 889
SfoI GGCGCC 1 cut(s) 1150
Sfr274I CTCGAG 1 cut(s) 306
SinI GGWCC 1 cut(s) 940
SlaI CTCGAG 1 cut(s) 306
SmiMI CAYNNNNRTG 2 cut(s) 510, 681
SmlI CTYRAG 1 cut(s) 306
SmoI CTYRAG 1 cut(s) 306
Sse9I AATT 9 cut(s) 25, 117, 162, 484, 701, 873, 973, 1179, 1192
SsiI CCGC 3 cut(s) 604, 781, 842
SspDI GGCGCC 1 cut(s) 1148
SspMI CTAG 2 cut(s) 323, 384
StyD4I CCNGG 6 cut(s) 194, 224, 328, 736, 823, 936
StyI CCWWGG 3 cut(s) 943, 1057, 1085
TaaI ACNGT 2 cut(s) 430, 633
TaiI ACGT 1 cut(s) 456
TaqI TCGA 3 cut(s) 307, 456, 548
TasI AATT 9 cut(s) 25, 117, 162, 484, 701, 873, 973, 1179, 1192
TatI WGTACW 1 cut(s) 189
TfiI GAWTC 2 cut(s) 136, 925
Tru1I TTAA 2 cut(s) 29, 468
Tru9I TTAA 2 cut(s) 29, 468
TscAI CASTG 3 cut(s) 66, 262, 841
TseFI GTSAC 1 cut(s) 61
TseI GCWGC 2 cut(s) 250, 461
Tsp45I GTSAC 1 cut(s) 61
TspDTI ATGAA 1 cut(s) 149
TspGWI ACGGA 1 cut(s) 132
TspRI CASTG 3 cut(s) 66, 262, 841
Tth111I GACNNNGTC 1 cut(s) 938
Van91I CCANNNNNTGG 1 cut(s) 755
VpaK11BI GGWCC 1 cut(s) 940
XapI RAATTY 3 cut(s) 25, 484, 1192
XceI RCATGY 1 cut(s) 808
XhoI CTCGAG 1 cut(s) 306
XspI CTAG 2 cut(s) 323, 384
ZrmI AGTACT 1 cut(s) 191
Zsp2I ATGCAT 1 cut(s) 904
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.