MD09G1051400.v1.1

Protein of unknown function (DUF3675)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0003694
Physical Location & Seq
Reverse (-)
3436192 .. 3437142
951 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1051400.v1.1.491

Sequence Viewer

Length: 333 bp
ATGGAAACCGCTAGCAAGATGGTGGAGAAAGCGGCGGCCTTCGCCTCAGGGAAAAGTAGTACTGAAGCAAAGGAGAAAGTAGAGGCGCTGAACAAAGACATAGAAGCAGGTCAGGGATCCTCGAATGTGAACTCGGAAGAAGGGCAGGGATCTGGTATTGGAATACAAAATGAATGCAGAATTTGCCAGGAGGAGGATTACCTTCACAAACTCGAAGCTCCTTGCCGCTGCAACGGCACTCTTAAGTTTGCTCACAGGGATTGTGTTCAAAAATGGATCAACGAGAGGCTAAGTATGACTTGTGAAATCTGCAACCAGGTATATATCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3675 RINGv

Protein Analysis

111

Amino Acids

12.13

Weight (kDa)

5.21

Isoelectric Point (pI)

33.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 59 - 104 6.2e-14 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 98
AciI CCGC 4 cut(s) 9, 32, 35, 226
AclWI GGATC 4 cut(s) 111, 124, 157, 284
AcsI RAATTY 1 cut(s) 180
AcuI CTGAAG 1 cut(s) 84
AfaI GTAC 1 cut(s) 61
AfiI CCNNNNNNNGG 1 cut(s) 193
AflII CTTAAG 1 cut(s) 242
AgsI TTSAA 1 cut(s) 269
AjnI CCWGG 2 cut(s) 186, 315
AluBI AGCT 1 cut(s) 218
AluI AGCT 1 cut(s) 218
AlwI GGATC 4 cut(s) 111, 124, 157, 284
AoxI GGCC 1 cut(s) 36
ApeKI GCWGC 1 cut(s) 228
ApoI RAATTY 1 cut(s) 180
AspLEI GCGC 1 cut(s) 88
AsuNHI GCTAGC 1 cut(s) 11
AxyI CCTNAGG 1 cut(s) 46
BamHI GGATCC 1 cut(s) 116
BbvI GCAGC 1 cut(s) 215
BccI CCATC 1 cut(s) 13
BceAI ACGGC 1 cut(s) 250
BciT130I CCWGG 2 cut(s) 188, 317
BfaI CTAG 1 cut(s) 12
BfoI RGCGCY 1 cut(s) 89
BfrI CTTAAG 1 cut(s) 242
BfuAI ACCTGC 1 cut(s) 98
BisI GCNGC 4 cut(s) 33, 36, 226, 229
BlsI GCNGC 4 cut(s) 34, 37, 227, 230
BmcAI AGTACT 1 cut(s) 61
Bme1390I CCNGG 2 cut(s) 188, 317
BmiI GGNNCC 1 cut(s) 118
BmrFI CCNGG 2 cut(s) 188, 317
BmtI GCTAGC 1 cut(s) 15
Bsc4I CCNNNNNNNGG 1 cut(s) 193
Bse21I CCTNAGG 1 cut(s) 46
BseBI CCWGG 2 cut(s) 188, 317
BseLI CCNNNNNNNGG 1 cut(s) 193
BseMII CTCAG 1 cut(s) 60
BseRI GAGGAG 1 cut(s) 206
BseXI GCAGC 1 cut(s) 215
BshFI GGCC 1 cut(s) 38
BslI CCNNNNNNNGG 1 cut(s) 193
BsmI GAATGC 1 cut(s) 179
BsnI GGCC 1 cut(s) 38
Bsp143I GATC 3 cut(s) 116, 149, 276
BspACI CCGC 4 cut(s) 9, 32, 35, 226
BspANI GGCC 1 cut(s) 38
BspCNI CTCAG 1 cut(s) 59
BspLI GGNNCC 1 cut(s) 118
BspMI ACCTGC 1 cut(s) 98
BspOI GCTAGC 1 cut(s) 15
BspPI GGATC 4 cut(s) 111, 124, 157, 284
BspTI CTTAAG 1 cut(s) 242
BssMI GATC 3 cut(s) 116, 149, 276
Bst2UI CCWGG 2 cut(s) 188, 317
BstAFI CTTAAG 1 cut(s) 242
BstAPI GCANNNNNTGC 1 cut(s) 183
BstC8I GCNNGC 1 cut(s) 13
BstDEI CTNAG 2 cut(s) 46, 290
BstH2I RGCGCY 1 cut(s) 89
BstHHI GCGC 1 cut(s) 88
BstKTI GATC 3 cut(s) 119, 152, 279
BstMBI GATC 3 cut(s) 116, 149, 276
BstMWI GCNNNNNNNGC 3 cut(s) 41, 183, 234
BstNI CCWGG 2 cut(s) 188, 317
BstSCI CCNGG 2 cut(s) 186, 315
BstV1I GCAGC 1 cut(s) 215
BstX2I RGATCY 2 cut(s) 116, 149
BstYI RGATCY 2 cut(s) 116, 149
Bsu36I CCTNAGG 1 cut(s) 46
BsuRI GGCC 1 cut(s) 38
BveI ACCTGC 1 cut(s) 98
Cac8I GCNNGC 1 cut(s) 13
CfoI GCGC 1 cut(s) 88
CsiI ACCWGGT 1 cut(s) 315
Csp6I GTAC 1 cut(s) 60
CviJI RGCY 3 cut(s) 38, 218, 289
CviKI_1 RGCY 3 cut(s) 38, 218, 289
CviQI GTAC 1 cut(s) 60
DdeI CTNAG 2 cut(s) 46, 290
DpnI GATC 3 cut(s) 118, 151, 278
DpnII GATC 3 cut(s) 116, 149, 276
Eco57I CTGAAG 1 cut(s) 84
Eco81I CCTNAGG 1 cut(s) 46
EcoRII CCWGG 2 cut(s) 186, 315
FaiI YATR 6 cut(s) 101, 296, 322, 324, 329, 331
FalI AAGNNNNNCTT 2 cut(s) 283, 315
Fnu4HI GCNGC 4 cut(s) 33, 36, 226, 229
Fsp4HI GCNGC 4 cut(s) 33, 36, 226, 229
FspBI CTAG 1 cut(s) 12
GlaI GCGC 1 cut(s) 87
GluI GCNGC 4 cut(s) 33, 36, 226, 229
HaeII RGCGCY 1 cut(s) 89
HaeIII GGCC 1 cut(s) 38
HhaI GCGC 1 cut(s) 88
Hin6I GCGC 1 cut(s) 86
HinP1I GCGC 1 cut(s) 86
Hpy166II GTNNAC 1 cut(s) 130
Hpy188I TCNGA 1 cut(s) 136
Hpy8I GTNNAC 1 cut(s) 130
HpyAV CCTTC 3 cut(s) 49, 134, 212
HpyCH4V TGCA 3 cut(s) 177, 231, 312
HpyF10VI GCNNNNNNNGC 3 cut(s) 41, 183, 234
HpyF3I CTNAG 2 cut(s) 46, 290
HspAI GCGC 1 cut(s) 86
Kzo9I GATC 3 cut(s) 116, 149, 276
LmnI GCTCC 1 cut(s) 223
Lsp1109I GCAGC 1 cut(s) 215
MabI ACCWGGT 1 cut(s) 315
MaeI CTAG 1 cut(s) 12
MalI GATC 3 cut(s) 118, 151, 278
MboI GATC 3 cut(s) 116, 149, 276
MboII GAAGA 1 cut(s) 149
MflI RGATCY 2 cut(s) 116, 149
MluCI AATT 1 cut(s) 180
MnlI CCTC 6 cut(s) 55, 76, 130, 184, 187, 279
MseI TTAA 1 cut(s) 243
MspA1I CMGCKG 1 cut(s) 228
MspCI CTTAAG 1 cut(s) 242
MspR9I CCNGG 2 cut(s) 188, 317
Mva1269I GAATGC 1 cut(s) 179
MvaI CCWGG 2 cut(s) 188, 317
MwoI GCNNNNNNNGC 3 cut(s) 41, 183, 234
NdeII GATC 3 cut(s) 116, 149, 276
NheI GCTAGC 1 cut(s) 11
NlaIV GGNNCC 1 cut(s) 118
PctI GAATGC 1 cut(s) 179
PkrI GCNGC 4 cut(s) 34, 37, 227, 230
Psp6I CCWGG 2 cut(s) 186, 315
PspGI CCWGG 2 cut(s) 186, 315
PspN4I GGNNCC 1 cut(s) 118
PsuI RGATCY 2 cut(s) 116, 149
RsaI GTAC 1 cut(s) 61
RsaNI GTAC 1 cut(s) 60
SaqAI TTAA 1 cut(s) 243
SatI GCNGC 4 cut(s) 33, 36, 226, 229
Sau3AI GATC 3 cut(s) 116, 149, 276
ScaI AGTACT 1 cut(s) 61
ScrFI CCNGG 2 cut(s) 188, 317
SetI ASST 4 cut(s) 112, 204, 220, 321
SexAI ACCWGGT 1 cut(s) 315
SmlI CTYRAG 1 cut(s) 242
SmoI CTYRAG 1 cut(s) 242
Sse9I AATT 1 cut(s) 180
SsiI CCGC 4 cut(s) 9, 32, 35, 226
SspMI CTAG 1 cut(s) 12
StyD4I CCNGG 2 cut(s) 186, 315
TaqI TCGA 2 cut(s) 122, 213
TasI AATT 1 cut(s) 180
TatI WGTACW 1 cut(s) 59
TauI GCSGC 3 cut(s) 35, 38, 228
Tru1I TTAA 1 cut(s) 243
Tru9I TTAA 1 cut(s) 243
TseI GCWGC 1 cut(s) 228
TspDTI ATGAA 1 cut(s) 186
Vha464I CTTAAG 1 cut(s) 242
XapI RAATTY 1 cut(s) 180
XspI CTAG 1 cut(s) 12
ZrmI AGTACT 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.