Rw5G034110

phospholipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0003694
Physical Location & Seq
Forward (+)
58536434 .. 58545241
8808 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G034110.1

Sequence Viewer

Length: 369 bp
ATGGATCATGAAGTGGTCATAGAAGCTGGGTGTGGATTAGAAGAATCGTCATGTGCTGAGTGTAAAATATGCCAGCAGAAGGACGTTATTGACAAAATGAACGCTCCATGTTATTGCAACAACACTTTAAAGTTTGCGCGTAGAGAATGCATACAAAAATGGATAAACTTGCGTCGCAAGACCATTTGCGAACTTTGTAAACATCCGTATGAAGGTGGTTATATCATAACAGTGCCTCCACAACGGCGTCCTACAAGGAGAAGGGAAGTGGAAAATCAAAGCGTGCAGGCGGAGGCAGCCGCAGCGGATGTAGCATGCCAAATTGCTTCCATCATAGTAAGCCACCTTAATTTAATTGAACTCAATTAA

Protein Analysis

122

Amino Acids

13.83

Weight (kDa)

6.8

Isoelectric Point (pI)

70.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 21 - 66 7.1e-09 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 139
AciI CCGC 3 cut(s) 290, 300, 305
AclWI GGATC 1 cut(s) 12
AcyI GRCGYC 1 cut(s) 247
AfiI CCNNNNNNNGG 2 cut(s) 79, 212
AgsI TTSAA 1 cut(s) 359
AluBI AGCT 1 cut(s) 26
AluI AGCT 1 cut(s) 26
AlwI GGATC 1 cut(s) 12
ApeKI GCWGC 2 cut(s) 296, 302
AspLEI GCGC 1 cut(s) 139
BbvI GCAGC 2 cut(s) 308, 314
BccI CCATC 1 cut(s) 338
BceAI ACGGC 1 cut(s) 260
BisI GCNGC 3 cut(s) 297, 300, 303
BlsI GCNGC 3 cut(s) 298, 301, 304
BsaHI GRCGYC 1 cut(s) 247
BsaXI ACNNNNNCTCC 2 cut(s) 220, 250
Bsc4I CCNNNNNNNGG 2 cut(s) 79, 212
BseGI GGATG 2 cut(s) 202, 313
BseLI CCNNNNNNNGG 2 cut(s) 79, 212
BseMII CTCAG 1 cut(s) 48
BseXI GCAGC 2 cut(s) 308, 314
BseYI CCCAGC 1 cut(s) 26
BsgI GTGCAG 1 cut(s) 305
Bsh1236I CGCG 1 cut(s) 139
BslI CCNNNNNNNGG 2 cut(s) 79, 212
BsmI GAATGC 1 cut(s) 152
Bsp143I GATC 1 cut(s) 4
BspACI CCGC 3 cut(s) 290, 300, 305
BspCNI CTCAG 1 cut(s) 49
BspFNI CGCG 1 cut(s) 139
BspHI TCATGA 1 cut(s) 7
BspPI GGATC 1 cut(s) 12
BssMI GATC 1 cut(s) 4
BssNI GRCGYC 1 cut(s) 247
Bst4CI ACNGT 1 cut(s) 232
BstACI GRCGYC 1 cut(s) 247
BstC8I GCNNGC 4 cut(s) 74, 284, 288, 316
BstDEI CTNAG 1 cut(s) 57
BstF5I GGATG 2 cut(s) 202, 313
BstFNI CGCG 1 cut(s) 139
BstHHI GCGC 1 cut(s) 139
BstKTI GATC 1 cut(s) 7
BstMBI GATC 1 cut(s) 4
BstMWI GCNNNNNNNGC 3 cut(s) 296, 302, 311
BstNSI RCATGY 1 cut(s) 318
BstUI CGCG 1 cut(s) 139
BstV1I GCAGC 2 cut(s) 308, 314
BtsCI GGATG 2 cut(s) 202, 313
BtsIMutI CAGTG 1 cut(s) 237
Cac8I GCNNGC 4 cut(s) 74, 284, 288, 316
CciI TCATGA 1 cut(s) 7
CfoI GCGC 1 cut(s) 139
CseI GACGC 2 cut(s) 161, 236
CviAII CATG 4 cut(s) 8, 51, 108, 315
CviJI RGCY 3 cut(s) 26, 299, 342
CviKI_1 RGCY 3 cut(s) 26, 299, 342
DdeI CTNAG 1 cut(s) 57
DpnI GATC 1 cut(s) 6
DpnII GATC 1 cut(s) 4
DraI TTTAAA 1 cut(s) 129
EciI GGCGGA 1 cut(s) 305
EcoT22I ATGCAT 1 cut(s) 152
FaeI CATG 4 cut(s) 11, 54, 111, 318
FatI CATG 4 cut(s) 7, 50, 107, 314
Fnu4HI GCNGC 3 cut(s) 297, 300, 303
FokI GGATG 2 cut(s) 189, 320
Fsp4HI GCNGC 3 cut(s) 297, 300, 303
GlaI GCGC 1 cut(s) 138
GluI GCNGC 3 cut(s) 297, 300, 303
GsaI CCCAGC 1 cut(s) 30
HgaI GACGC 2 cut(s) 161, 236
HhaI GCGC 1 cut(s) 139
Hin1I GRCGYC 1 cut(s) 247
Hin1II CATG 4 cut(s) 11, 54, 111, 318
Hin6I GCGC 1 cut(s) 137
HinP1I GCGC 1 cut(s) 137
HinfI GANTC 1 cut(s) 44
Hpy166II GTNNAC 1 cut(s) 200
Hpy188III TCNNGA 1 cut(s) 8
Hpy8I GTNNAC 1 cut(s) 200
Hpy99I CGWCG 1 cut(s) 177
HpyAV CCTTC 3 cut(s) 73, 206, 255
HpyCH4III ACNGT 1 cut(s) 232
HpyCH4IV ACGT 1 cut(s) 84
HpyCH4V TGCA 3 cut(s) 117, 150, 286
HpyF10VI GCNNNNNNNGC 3 cut(s) 296, 302, 311
HpyF3I CTNAG 1 cut(s) 57
HpySE526I ACGT 1 cut(s) 84
Hsp92I GRCGYC 1 cut(s) 247
Hsp92II CATG 4 cut(s) 11, 54, 111, 318
HspAI GCGC 1 cut(s) 137
Kzo9I GATC 1 cut(s) 4
LmnI GCTCC 1 cut(s) 109
LpnPI CCDG 3 cut(s) 12, 86, 272
Lsp1109I GCAGC 2 cut(s) 308, 314
MaeII ACGT 1 cut(s) 84
MalI GATC 1 cut(s) 6
MboI GATC 1 cut(s) 4
MboII GAAGA 1 cut(s) 53
MluCI AATT 4 cut(s) 321, 349, 354, 364
MnlI CCTC 2 cut(s) 246, 286
Mph1103I ATGCAT 1 cut(s) 152
MseI TTAA 4 cut(s) 128, 348, 353, 367
MslI CAYNNNNRTG 2 cut(s) 207, 230
MspA1I CMGCKG 1 cut(s) 305
Mva1269I GAATGC 1 cut(s) 152
MvnI CGCG 1 cut(s) 139
MwoI GCNNNNNNNGC 3 cut(s) 296, 302, 311
NdeII GATC 1 cut(s) 4
NlaIII CATG 4 cut(s) 11, 54, 111, 318
NsiI ATGCAT 1 cut(s) 152
NspI RCATGY 1 cut(s) 318
PaeI GCATGC 1 cut(s) 318
PagI TCATGA 1 cut(s) 7
PctI GAATGC 1 cut(s) 152
PfeI GAWTC 1 cut(s) 44
PkrI GCNGC 3 cut(s) 298, 301, 304
PspFI CCCAGC 1 cut(s) 26
RseI CAYNNNNRTG 2 cut(s) 207, 230
SaqAI TTAA 4 cut(s) 128, 348, 353, 367
SatI GCNGC 3 cut(s) 297, 300, 303
Sau3AI GATC 1 cut(s) 4
SetI ASST 4 cut(s) 28, 87, 217, 348
SmiMI CAYNNNNRTG 2 cut(s) 207, 230
SphI GCATGC 1 cut(s) 318
Sse9I AATT 4 cut(s) 321, 349, 354, 364
SsiI CCGC 3 cut(s) 290, 300, 305
TaaI ACNGT 1 cut(s) 232
TaiI ACGT 1 cut(s) 87
TasI AATT 4 cut(s) 321, 349, 354, 364
TauI GCSGC 1 cut(s) 302
TfiI GAWTC 1 cut(s) 44
Tru1I TTAA 4 cut(s) 128, 348, 353, 367
Tru9I TTAA 4 cut(s) 128, 348, 353, 367
TscAI CASTG 1 cut(s) 237
TseI GCWGC 2 cut(s) 296, 302
TspDTI ATGAA 3 cut(s) 24, 113, 225
TspGWI ACGGA 1 cut(s) 195
TspRI CASTG 1 cut(s) 237
XceI RCATGY 1 cut(s) 318
Zsp2I ATGCAT 1 cut(s) 152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.