Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0001061

Physical Location & Seq

Genomic Coordinates Reverse (-)

4787485 .. 4792584

Gene Structure 5100 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1069900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2835 bp

ATGGAGGTCTTACTGATGAACAGGCCTACTGGCCATGTATGCAAGACAAGAACACTGCTTGCATTGATCTTGTGTATGTGGGTGTCCATGCAAGTGATGGCTGGGACAGAAAATGCTACGCATTCGTCTGCAAGGCCGAGTTCTCTGAATTTTGGAGCTCTGTTTACTTTCAATTCGGTTATTGGGAGGGCAGCCAAACCGGCAATTTTAGCTGCAATTGATGATGTCAATTCTGATCAAAGCGTTCTTCGTGGGACGAAATTGAAGATTATTCTCCATGACACGAATTGCAGTGGATTTCTTGGAACTGTTGAAGCTCTGGAGCTGATTGAAAACGATGTGGTTGCTGCAATCGGCCCACAATCCTCCGGAATAGCTCATGTCATATCCCATGTTGTTAATGAGCTCCATGTACCACTTCTATCATTTGGAGCAACAGACCCCTCTCTTGCTGCTCTGCAATACCCATATTTTGTCCGAACCACACAGAGTGACTATTTCCAAATGTATGCAGTTGCTGATTTAGTTGAGTATTTTGGATGGAGAGAAGTAATAGCAATCTTTGTAGATGATGATTGTGGCAGAAATGGGATTTCTATACTAGGCGATGCCTTGGCGACGAAGCGTTCTAAGATCTCATACAAGGCTGCCTTCTCTCCTGGAGCCCCCAAAAGTGATATCAATGAATTGTTGGTGGGAGTGAACCTTATGGAATCTCGGGTTTATATTGTACATGTTAATCCTGACTCCGGTTTAACAATTTTCTCCATTGCCAAGGCACTTGGAATGATGACCAGTGGCTACGTCTGGATTGCAACGGATTGGCTTCCTTCTCATTTGGATTCACTAAATCCCCCTGGCCCCGACACAATGAATCTATTACAAGGGGTTGTTGCTCTTCGTCATCATACCCCAGATACTGATCTCAAAAAGCGCTTTATGTCTAGATGGAGCAAATTGAAACATGAAGGGACTCCAAGCTTCAATTCTTATGCGCTCTATGCATATGACTCTATTTGGCTAGCAGCCCGTGCTCTTGATGTTTTCTTCAACGAAGGTGGGAATGTATCTTTCTCTAATGACCCAAGGTTGAAAGACACAAATCGAAGCACACTGCATTTAACATCACTCCGTATTTTTGATGGAGGCCAAAAATACCTACAGACGATTCTTAAGATGAACTTCACAGGTATAAGTGGTCAGATTGAATTTGATCAAGATAAATATCTAGTTCGTCCAGCATATGACATTCTGAATATTGGAGGAACTGGTTCCCGTAGAGTTGGCTATTGGTCAAATTCTACTGGTCTCTCAGTCACTGCTCCAGAGATATTATATAAGAAGCCATCCAATAGGAATACCACTGCTCAACTTTATGGCGTTATATGGCCTGGTGAAGTTACAGCTACTCCTCGGGGATGGGTATTTCCCAACAATGGCAGGCCACTGCGAATTGGAGTGCCTTATCGAGTAAGTTACAAAGATTTTGTGGCTAAAGACAAGAGCCCTCCAGGGGTCAGAGGATACTGTATTGATGTCTTTGAAGCTGCTGTAAACTTGTTGCCGTATGCTGTGCCACGGATGTATATGTTGTATGGAGATGGTAAGAGGAATCCTGAGTACAGCAGTCTTGTAGCCCAGGTTGCGCAAAATAACTTTGATGCAGCTGTTGGAGATGTTACAATTACTACTAATAGGACAAGAATAGTTGATTTTACGCAGCCTTACATGGAATCGGGACTTGTTGTAGTTGTTCCTGTCAAAGAGGCAAAATCAAACCCTTGGGCTTTCCTCAAGCCATTTACTTATCAGATGTGGTTGGTCACAGGTGCCTTCTTCCTTTTAGTGGGAGCTGTTGTTTGGATTCTTGAGCACCGGATTAATCATGAGTTCCGTGGTCCACCGAGCCAACAGCTCATGACCATTTTTTGGTTTAGTTTCTCCACAATGTTTTTCTCACACAGAGAGAACACTGTGAGCACCCTGGGACGGCTGGTGCTGATTATATGGTTATTTGTAGTGTTAATTATCAATTCCAGCTACACGGCTAGTTTGACTTCAATTCTCACGGTGCAACAGCTGACGTCACGGATTGAAGGGATTGACAGCTTGATATCAAGTAATGATCGAATCGGAGTTCAAGACGGGTCATTCGCTTGGAGGTATTTGATTGATGAGATGAACATAGCGGAATCTAGACTCGTTAAGTTGAAAGACATGGAAGCCTATATTAAAGCCCTCACGGATGGACCAAGACGTGGTGGGGTAGCTGCCATCGTTGATGAGCTTCCTTACATTGAGTTGTTCATGTCCAACACCAAATGCGCATTCAGGACTGTAGGGCAGGAGTTTACCAAAAGCGGATGGGGATTCGCATTCCAAAGGGATTCTCCTCTTGCTGTTGACTTGTCGACTGCGATTCTTCAACTCTCAGAGAACGGCGAGCTACAAAAGATCCATAATAAGTGGCTTACGCACAACGAGTGCTCTACTCAACTCACTGAAGTTGATGCAGACCAGCTATCTCTGACAAGCTTTTGGGGCCTGTTTCTTATCTGCGGTATTGCATGCTTCCTTGCTCTTGCGGTGTTCTTCTGTAGAATCCTTTGTCAATACCGCAGATTTACCCCAGAGCCTGTGGAAGCGGACGCTGAGGAGATTGGACCTACCAACACGAGATCTAGACGCTCACTTGGGTCAACCAGTTTCAAGGGTCTTATGGTCTTTGTAGATAAGAAAGAAGCAGAGATCAAGCACATGCTTAAGCGAAAAACTAGTGATAGTAAGCACGAAGCTAGCCCGAGCACTGATGGGCAGCTGCATTCGCCTTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0004888 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0007154 GO:0007165 GO:0008066 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009507 GO:0009536 GO:0009605 GO:0009611 GO:0009612 GO:0009628 GO:0009719 GO:0009987 GO:0010033 GO:0010034 GO:0010243 GO:0015075 GO:0015085 GO:0015267 GO:0015276 GO:0015318 GO:0016020 GO:0019722 GO:0019932 GO:0022803 GO:0022834 GO:0022836 GO:0022838 GO:0022839 GO:0022857 GO:0022890 GO:0023052 GO:0030001 GO:0033554 GO:0034220 GO:0035556 GO:0038023 GO:0042221 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046677 GO:0046873 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055085 GO:0060089 GO:0065007 GO:0070417 GO:0070588 GO:0070838 GO:0070887 GO:0071214 GO:0071229 GO:0071230 GO:0071236 GO:0071260 GO:0071310 GO:0071311 GO:0071417 GO:0071495 GO:0071496 GO:0071944 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:0104004 GO:1901698 GO:1901699 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

945

Amino Acids

105.05

Weight (kDa)

7.29

Isoelectric Point (pI)

37.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peripla_BP_6	PF13458	60 - 346	2.4e-18	Periplasmic binding protein
ANF_receptor	PF01094	65 - 422	7.6e-90	Receptor family ligand binding region
Lig_chan-Glu_bd	PF10613	504 - 587	1.5e-11	Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3	PF00497	540 - 825	1.2e-12	Bacterial extracellular solute-binding proteins, family 3
Lig_chan	PF00060	603 - 857	4.7e-40	Ligand-gated ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0001061)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G05200 AT1G05200 AT1G05200 AT1G05200 AT1G05200 AT1G05200 AT1G05200 AT2G32390 AT2G32390 AT2G32390 AT2G32390 AT2G32390 AT2G32390 AT2G32390
fragaria_vesca	FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291
malus_domestica	MD09G1069900.v1.1 MD09G1070100.v1.1 MD09G1107700.v1.1 MD09G1108100.v1.1 MD17G1096100.v1.1
prunus_persica	Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1
pyrus_communis	pycom09g03000 pycom09g03030 pycom09g03050 pycom09g03070 pycom15g20430 pycom17g09220
rosa_chinensis	RchiOBHm_Chr1g0324131 RchiOBHm_Chr2g0159571 RchiOBHm_Chr2g0159581
rosa_laevigata	RLG00000021167
rosa_multiflora	Rmu_co8397097.1_g000001 Rmu_sc0004966.1_g000017 Rmu_sc0020031.1_g000001
rosa_roxburghii	Rroxscaffold_2G00090390
rosa_rugosa	Rorug02G0476900 Rorug02G0477000 Rorug02G0477100 Rorug02G0477200
rosa_samantha	Rh2AG544200 Rh2BG557000 Rh2CG527200 Rh2DG566000
rosa_wichuraiana	Rw2G044990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	2087
Acc16I	TGCGCA	2 cut(s)	1647, 2326
AccB1I	GGYRCC	1 cut(s)	1829
AccB7I	CCANNNNNTGG	2 cut(s)	1929, 2258
AccI	GTMKAC	1 cut(s)	2411
AccIII	TCCGGA	1 cut(s)	368
AciI	CCGC	6 cut(s)	2189, 2361, 2559, 2585, 2617, 2645
AclWI	GGATC	1 cut(s)	2449
AcoI	YGGCCR	1 cut(s)	31
AcsI	RAATTY	3 cut(s)	148, 1208, 1297
AcuI	CTGAAG	1 cut(s)	2523
AcyI	GRCGYC	1 cut(s)	2084
AdeI	CACNNNGTG	1 cut(s)	491
AfaI	GTAC	3 cut(s)	414, 732, 1622
AfeI	AGCGCT	1 cut(s)	935
AfiI	CCNNNNNNNGG	7 cut(s)	749, 1436, 1513, 1846, 1929, 1989, 2258
AflII	CTTAAG	2 cut(s)	1172, 2762
AflIII	ACRYGT	1 cut(s)	733
AhlI	ACTAGT	1 cut(s)	2774
AjiI	CACGTC	1 cut(s)	2258
AjnI	CCWGG	6 cut(s)	658, 856, 1390, 1510, 1638, 1983
AjuI	GAANNNNNNNTTGG	4 cut(s)	970, 1002, 2312, 2344
Alw21I	GWGCWC	7 cut(s)	160, 408, 1036, 1875, 1982, 2489, 2807
Alw26I	GTCTC	1 cut(s)	1313
AlwI	GGATC	1 cut(s)	2449
AlwNI	CAGNNNCTG	4 cut(s)	518, 920, 1319, 2636
Ama87I	CYCGRG	3 cut(s)	717, 1413, 2800
Aor13HI	TCCGGA	1 cut(s)	368
Aor51HI	AGCGCT	1 cut(s)	935
AoxI	GGCC	9 cut(s)	23, 31, 134, 355, 859, 1147, 1388, 1442, 2542
ApoI	RAATTY	3 cut(s)	148, 1208, 1297
ArsI	GACNNNNNNTTYG	2 cut(s)	2135, 2167
AseI	ATTAAT	1 cut(s)	1881
Asp700I	GAANNNNTTC	1 cut(s)	1270
AspLEI	GCGC	4 cut(s)	936, 997, 1648, 2327
AspS9I	GGNCC	6 cut(s)	356, 860, 1898, 2249, 2542, 2663
AsuHPI	GGTGA	1 cut(s)	1406
AsuNHI	GCTAGC	2 cut(s)	1021, 2795
AvaI	CYCGRG	3 cut(s)	717, 1413, 2800
AvaII	GGWCC	3 cut(s)	1898, 2249, 2663
BalI	TGGCCA	1 cut(s)	33
BanI	GGYRCC	1 cut(s)	1829
BanII	GRGCYC	4 cut(s)	160, 408, 667, 1508
BauI	CACGAG	1 cut(s)	2674
Bbv12I	GWGCWC	7 cut(s)	160, 408, 1036, 1875, 1982, 2489, 2807
BbvCI	CCTCAGC	1 cut(s)	2652
BceAI	ACGGC	4 cut(s)	1549, 2006, 2061, 2455
BcgI	CGANNNNNNTGC	2 cut(s)	326, 360
BciT130I	CCWGG	6 cut(s)	660, 858, 1392, 1512, 1640, 1985
BciVI	GTATCC	1 cut(s)	1517
BclI	TGATCA	2 cut(s)	235, 1213
BcoDI	GTCTC	1 cut(s)	1313
BcuI	ACTAGT	1 cut(s)	2774
BfaI	CTAG	9 cut(s)	602, 945, 1022, 1229, 2049, 2196, 2682, 2775, 2796
BfmI	CTRYAG	3 cut(s)	1160, 2337, 2596
BfoI	RGCGCY	1 cut(s)	937
BfrI	CTTAAG	2 cut(s)	1172, 2762
BfuI	GTATCC	1 cut(s)	1517
BglI	GCCNNNNNGGC	1 cut(s)	200
BglII	AGATCT	2 cut(s)	633, 2678
Bme1390I	CCNGG	6 cut(s)	660, 858, 1392, 1512, 1640, 1985
Bme18I	GGWCC	3 cut(s)	1898, 2249, 2663
BmeT110I	CYCGRG	3 cut(s)	717, 1413, 2800
BmgBI	CACGTC	1 cut(s)	2258
BmgT120I	GGNCC	6 cut(s)	356, 860, 1898, 2249, 2542, 2663
BmiI	GGNNCC	5 cut(s)	664, 862, 1273, 1831, 2543
BmrFI	CCNGG	6 cut(s)	660, 858, 1392, 1512, 1640, 1985
BmsI	GCATC	3 cut(s)	598, 1651, 2500
BmtI	GCTAGC	2 cut(s)	1025, 2799
BpmI	CTGGAG	4 cut(s)	341, 681, 1308, 1494
Bpu10I	CCTNAGC	1 cut(s)	2652
BpuEI	CTTGAG	2 cut(s)	1778, 1889
BsaBI	GATNNNNATC	2 cut(s)	921, 1224
BsaHI	GRCGYC	1 cut(s)	2084
BsaI	GGTCTC	1 cut(s)	1313
BsaWI	WCCGGW	3 cut(s)	368, 749, 1875
BsaXI	ACNNNNNCTCC	4 cut(s)	1393, 1423, 1842, 1872
Bsc4I	CCNNNNNNNGG	7 cut(s)	749, 1436, 1513, 1846, 1929, 1989, 2258
Bse118I	RCCGGY	1 cut(s)	199
Bse1I	ACTGG	5 cut(s)	34, 795, 1273, 1309, 2703
Bse3DI	GCAATG	1 cut(s)	768
Bse8I	GATNNNNATC	2 cut(s)	921, 1224
BseAI	TCCGGA	1 cut(s)	368
BseBI	CCWGG	6 cut(s)	660, 858, 1392, 1512, 1640, 1985
BseGI	GGATG	6 cut(s)	545, 1346, 1424, 1587, 2251, 2369
BseJI	GATNNNNATC	2 cut(s)	921, 1224
BseLI	CCNNNNNNNGG	7 cut(s)	749, 1436, 1513, 1846, 1929, 1989, 2258
BseMI	GCAATG	1 cut(s)	768
BseMII	CTCAG	4 cut(s)	1326, 1608, 2445, 2643
BseNI	ACTGG	5 cut(s)	34, 795, 1273, 1309, 2703
BseRI	GAGGAG	3 cut(s)	1401, 2382, 2669
BseYI	CCCAGC	1 cut(s)	101
BshFI	GGCC	9 cut(s)	25, 33, 136, 357, 861, 1149, 1390, 1444, 2544
BshNI	GGYRCC	1 cut(s)	1829
BsiHKAI	GWGCWC	7 cut(s)	160, 408, 1036, 1875, 1982, 2489, 2807
BsiHKCI	CYCGRG	3 cut(s)	717, 1413, 2800
BsiSI	CCGG	4 cut(s)	200, 369, 750, 1876
BslFI	GGGAC	5 cut(s)	118, 268, 985, 1752, 2001
BslI	CCNNNNNNNGG	7 cut(s)	749, 1436, 1513, 1846, 1929, 1989, 2258
BsmAI	GTCTC	1 cut(s)	1313
BsmFI	GGGAC	5 cut(s)	118, 268, 985, 1752, 2001
BsmI	GAATGC	4 cut(s)	121, 2327, 2375, 2821
BsnI	GGCC	9 cut(s)	25, 33, 136, 357, 861, 1149, 1390, 1444, 2544
Bso31I	GGTCTC	1 cut(s)	1313
BsoBI	CYCGRG	3 cut(s)	717, 1413, 2800
Bsp1286I	GDGCHC	9 cut(s)	160, 408, 667, 1036, 1508, 1875, 1982, 2489, 2807
Bsp13I	TCCGGA	1 cut(s)	368
Bsp1407I	TGTACA	1 cut(s)	730
Bsp143I	GATC	9 cut(s)	66, 235, 633, 922, 1213, 2125, 2454, 2678, 2748
BspACI	CCGC	6 cut(s)	2189, 2361, 2559, 2585, 2617, 2645
BspANI	GGCC	9 cut(s)	25, 33, 136, 357, 861, 1149, 1390, 1444, 2544
BspCNI	CTCAG	4 cut(s)	1325, 1609, 2444, 2644
BspEI	TCCGGA	1 cut(s)	368
BspHI	TCATGA	2 cut(s)	1885, 1917
BspLI	GGNNCC	5 cut(s)	664, 862, 1273, 1831, 2543
BspOI	GCTAGC	2 cut(s)	1025, 2799
BspPI	GGATC	1 cut(s)	2449
BspQI	GCTCTTC	1 cut(s)	903
BspT107I	GGYRCC	1 cut(s)	1829
BspTI	CTTAAG	2 cut(s)	1172, 2762
BspTNI	GGTCTC	1 cut(s)	1313
BsrDI	GCAATG	1 cut(s)	768
BsrFI	RCCGGY	1 cut(s)	199
BsrGI	TGTACA	1 cut(s)	730
BsrI	ACTGG	5 cut(s)	34, 795, 1273, 1309, 2703
BssAI	RCCGGY	1 cut(s)	199
BssMI	GATC	9 cut(s)	66, 235, 633, 922, 1213, 2125, 2454, 2678, 2748
BssNI	GRCGYC	1 cut(s)	2084
BssSI	CACGAG	1 cut(s)	2674
BssT1I	CCWWGG	4 cut(s)	612, 774, 1085, 1781
Bst2BI	CACGAG	1 cut(s)	2674
Bst2UI	CCWGG	6 cut(s)	660, 858, 1392, 1512, 1640, 1985
Bst4CI	ACNGT	5 cut(s)	310, 1529, 1975, 2071, 2338
Bst6I	CTCTTC	1 cut(s)	903
BstACI	GRCGYC	1 cut(s)	2084
BstAFI	CTTAAG	2 cut(s)	1172, 2762
BstAPI	GCANNNNNTGC	1 cut(s)	1031
BstAUI	TGTACA	1 cut(s)	730
BstC8I	GCNNGC	6 cut(s)	60, 1023, 1442, 2444, 2569, 2797
BstDEI	CTNAG	5 cut(s)	630, 1312, 1617, 2431, 2652
BstDSI	CCRYGG	2 cut(s)	1577, 1894
BstF5I	GGATG	6 cut(s)	545, 1346, 1424, 1587, 2251, 2369
BstH2I	RGCGCY	1 cut(s)	937
BstHHI	GCGC	4 cut(s)	936, 997, 1648, 2327
BstKTI	GATC	9 cut(s)	69, 238, 636, 925, 1216, 2128, 2457, 2681, 2751
BstMAI	GTCTC	1 cut(s)	1313
BstMBI	GATC	9 cut(s)	66, 235, 633, 922, 1213, 2125, 2454, 2678, 2748
BstMWI	GCNNNNNNNGC	8 cut(s)	39, 200, 209, 1001, 1031, 1643, 2541, 2824
BstNI	CCWGG	6 cut(s)	660, 858, 1392, 1512, 1640, 1985
BstNSI	RCATGY	3 cut(s)	737, 2571, 2761
BstSCI	CCNGG	6 cut(s)	658, 856, 1390, 1510, 1638, 1983
BstSFI	CTRYAG	3 cut(s)	1160, 2337, 2596
BstX2I	RGATCY	3 cut(s)	633, 2454, 2678
BstYI	RGATCY	3 cut(s)	633, 2454, 2678
BsuI	GTATCC	1 cut(s)	1517
BsuRI	GGCC	9 cut(s)	25, 33, 136, 357, 861, 1149, 1390, 1444, 2544
BtgI	CCRYGG	2 cut(s)	1577, 1894
BtgZI	GCGATG	1 cut(s)	621
BtrI	CACGTC	1 cut(s)	2258
BtsCI	GGATG	6 cut(s)	545, 1346, 1424, 1587, 2251, 2369
BtsI	GCAGTG	6 cut(s)	53, 298, 1112, 1317, 1362, 1445
Cac8I	GCNNGC	6 cut(s)	60, 1023, 1442, 2444, 2569, 2797
CaiI	CAGNNNCTG	4 cut(s)	518, 920, 1319, 2636
CciI	TCATGA	2 cut(s)	1885, 1917
CfoI	GCGC	4 cut(s)	936, 997, 1648, 2327
Cfr10I	RCCGGY	1 cut(s)	199
Cfr13I	GGNCC	6 cut(s)	356, 860, 1898, 2249, 2542, 2663
CseI	GACGC	2 cut(s)	2657, 2694
Csp6I	GTAC	3 cut(s)	413, 731, 1621
CspCI	CAANNNNNGTGG	2 cut(s)	1039, 1074
CviQI	GTAC	3 cut(s)	413, 731, 1621
DdeI	CTNAG	5 cut(s)	630, 1312, 1617, 2431, 2652
DpnI	GATC	9 cut(s)	68, 237, 635, 924, 1215, 2127, 2456, 2680, 2750
DpnII	GATC	9 cut(s)	66, 235, 633, 922, 1213, 2125, 2454, 2678, 2748
DraIII	CACNNNGTG	1 cut(s)	491
EaeI	YGGCCR	1 cut(s)	31
Eam1104I	CTCTTC	1 cut(s)	903
EarI	CTCTTC	1 cut(s)	903
Ecl136II	GAGCTC	2 cut(s)	158, 406
Eco130I	CCWWGG	4 cut(s)	612, 774, 1085, 1781
Eco147I	AGGCCT	1 cut(s)	25
Eco24I	GRGCYC	4 cut(s)	160, 408, 667, 1508
Eco31I	GGTCTC	1 cut(s)	1313
Eco32I	GATATC	2 cut(s)	679, 2115
Eco47I	GGWCC	3 cut(s)	1898, 2249, 2663
Eco47III	AGCGCT	1 cut(s)	935
Eco53kI	GAGCTC	2 cut(s)	158, 406
Eco57I	CTGAAG	1 cut(s)	2523
Eco88I	CYCGRG	3 cut(s)	717, 1413, 2800
EcoICRI	GAGCTC	2 cut(s)	158, 406
EcoO109I	RGGNCCY	1 cut(s)	2542
EcoRII	CCWGG	6 cut(s)	658, 856, 1390, 1510, 1638, 1983
EcoRV	GATATC	2 cut(s)	679, 2115
EcoT14I	CCWWGG	4 cut(s)	612, 774, 1085, 1781
EcoT22I	ATGCAT	1 cut(s)	1006
EcoT38I	GRGCYC	4 cut(s)	160, 408, 667, 1508
ErhI	CCWWGG	4 cut(s)	612, 774, 1085, 1781
FalI	AAGNNNNNCTT	4 cut(s)	635, 667, 1166, 1198
FaqI	GGGAC	5 cut(s)	118, 268, 985, 1752, 2001
FauNDI	CATATG	2 cut(s)	1006, 1243
FbaI	TGATCA	2 cut(s)	235, 1213
FblI	GTMKAC	1 cut(s)	2411
FokI	GGATG	6 cut(s)	552, 1333, 1431, 1594, 2258, 2376
FriOI	GRGCYC	4 cut(s)	160, 408, 667, 1508
FspAI	RTGCGCAY	1 cut(s)	2326
FspBI	CTAG	9 cut(s)	602, 945, 1022, 1229, 2049, 2196, 2682, 2775, 2796
FspI	TGCGCA	2 cut(s)	1647, 2326
GlaI	GCGC	4 cut(s)	935, 996, 1647, 2326
GsaI	CCCAGC	1 cut(s)	105
GsuI	CTGGAG	4 cut(s)	341, 681, 1308, 1494
HaeII	RGCGCY	1 cut(s)	937
HaeIII	GGCC	9 cut(s)	25, 33, 136, 357, 861, 1149, 1390, 1444, 2544
HapII	CCGG	4 cut(s)	200, 369, 750, 1876
HgaI	GACGC	2 cut(s)	2657, 2694
HhaI	GCGC	4 cut(s)	936, 997, 1648, 2327
Hin1I	GRCGYC	1 cut(s)	2084
Hin6I	GCGC	4 cut(s)	934, 995, 1646, 2325
HinP1I	GCGC	4 cut(s)	934, 995, 1646, 2325
HincII	GTYRAC	3 cut(s)	2404, 2412, 2700
HindII	GTYRAC	3 cut(s)	2404, 2412, 2700
HindIII	AAGCTT	2 cut(s)	979, 2533
HpaII	CCGG	4 cut(s)	200, 369, 750, 1876
HphI	GGTGA	1 cut(s)	1406
Hpy166II	GTNNAC	8 cut(s)	165, 703, 1555, 1901, 2352, 2404, 2412, 2700
Hpy8I	GTNNAC	8 cut(s)	165, 703, 1555, 1901, 2352, 2404, 2412, 2700
Hpy99I	CGWCG	1 cut(s)	622
HpyAV	CCTTC	6 cut(s)	661, 840, 962, 1049, 1843, 2090
HpyCH4III	ACNGT	5 cut(s)	310, 1529, 1975, 2071, 2338
HpyCH4IV	ACGT	3 cut(s)	804, 2084, 2257
HpyF10VI	GCNNNNNNNGC	8 cut(s)	39, 200, 209, 1001, 1031, 1643, 2541, 2824
HpyF3I	CTNAG	5 cut(s)	630, 1312, 1617, 2431, 2652
HpySE526I	ACGT	3 cut(s)	804, 2084, 2257
Hsp92I	GRCGYC	1 cut(s)	2084
HspAI	GCGC	4 cut(s)	934, 995, 1646, 2325
Kpn2I	TCCGGA	1 cut(s)	368
Ksp22I	TGATCA	2 cut(s)	235, 1213
Kzo9I	GATC	9 cut(s)	66, 235, 633, 922, 1213, 2125, 2454, 2678, 2748
LguI	GCTCTTC	1 cut(s)	903
LmnI	GCTCC	8 cut(s)	155, 322, 411, 431, 662, 951, 1327, 1850
LweI	GCATC	3 cut(s)	598, 1651, 2500
MaeI	CTAG	9 cut(s)	602, 945, 1022, 1229, 2049, 2196, 2682, 2775, 2796
MaeII	ACGT	3 cut(s)	804, 2084, 2257
MaeIII	GTNAC	7 cut(s)	491, 1315, 1399, 1475, 1678, 1822, 2085
MalI	GATC	9 cut(s)	68, 237, 635, 924, 1215, 2127, 2456, 2680, 2750
MboI	GATC	9 cut(s)	66, 235, 633, 922, 1213, 2125, 2454, 2678, 2748
MboII	GAAGA	7 cut(s)	239, 277, 890, 1039, 1828, 2414, 2584
MfeI	CAATTG	1 cut(s)	216
MflI	RGATCY	3 cut(s)	633, 2454, 2678
MhlI	GDGCHC	9 cut(s)	160, 408, 667, 1036, 1508, 1875, 1982, 2489, 2807
MlsI	TGGCCA	1 cut(s)	33
MluNI	TGGCCA	1 cut(s)	33
MlyI	GAGTC	4 cut(s)	740, 967, 1004, 2193
MmeI	TCCRAC	2 cut(s)	1651, 2337
Mox20I	TGGCCA	1 cut(s)	33
Mph1103I	ATGCAT	1 cut(s)	1006
MroI	TCCGGA	1 cut(s)	368
MroXI	GAANNNNTTC	1 cut(s)	1270
MscI	TGGCCA	1 cut(s)	33
MslI	CAYNNNNRTG	1 cut(s)	92
Msp20I	TGGCCA	1 cut(s)	33
MspA1I	CMGCKG	3 cut(s)	1667, 2080, 2818
MspCI	CTTAAG	2 cut(s)	1172, 2762
MspI	CCGG	4 cut(s)	200, 369, 750, 1876
MspR9I	CCNGG	6 cut(s)	660, 858, 1392, 1512, 1640, 1985
MunI	CAATTG	1 cut(s)	216
Mva1269I	GAATGC	4 cut(s)	121, 2327, 2375, 2821
MvaI	CCWGG	6 cut(s)	660, 858, 1392, 1512, 1640, 1985
MwoI	GCNNNNNNNGC	8 cut(s)	39, 200, 209, 1001, 1031, 1643, 2541, 2824
NdeI	CATATG	2 cut(s)	1006, 1243
NdeII	GATC	9 cut(s)	66, 235, 633, 922, 1213, 2125, 2454, 2678, 2748
NheI	GCTAGC	2 cut(s)	1021, 2795
NlaIV	GGNNCC	5 cut(s)	664, 862, 1273, 1831, 2543
NmeAIII	GCCGAG	1 cut(s)	162
NmuCI	GTSAC	4 cut(s)	491, 1315, 1822, 2085
NsbI	TGCGCA	2 cut(s)	1647, 2326
NsiI	ATGCAT	1 cut(s)	1006
NspI	RCATGY	3 cut(s)	737, 2571, 2761
PaeI	GCATGC	1 cut(s)	2571
PagI	TCATGA	2 cut(s)	1885, 1917
PasI	CCCWGGG	1 cut(s)	1984
PceI	AGGCCT	1 cut(s)	25
PciI	ACATGT	1 cut(s)	733
PciSI	GCTCTTC	1 cut(s)	903
PctI	GAATGC	4 cut(s)	121, 2327, 2375, 2821
PdmI	GAANNNNTTC	1 cut(s)	1270
PflMI	CCANNNNNTGG	2 cut(s)	1929, 2258
PfoI	TCCNGGA	1 cut(s)	658
PleI	GAGTC	4 cut(s)	740, 967, 1004, 2193
PpsI	GAGTC	4 cut(s)	740, 967, 1004, 2193
PscI	ACATGT	1 cut(s)	733
PshBI	ATTAAT	1 cut(s)	1881
Psp124BI	GAGCTC	2 cut(s)	160, 408
Psp6I	CCWGG	6 cut(s)	658, 856, 1390, 1510, 1638, 1983
PspFI	CCCAGC	1 cut(s)	101
PspGI	CCWGG	6 cut(s)	658, 856, 1390, 1510, 1638, 1983
PspN4I	GGNNCC	5 cut(s)	664, 862, 1273, 1831, 2543
PspPI	GGNCC	6 cut(s)	356, 860, 1898, 2249, 2542, 2663
PstNI	CAGNNNCTG	4 cut(s)	518, 920, 1319, 2636
PsuI	RGATCY	3 cut(s)	633, 2454, 2678
PvuII	CAGCTG	3 cut(s)	1667, 2080, 2818
RsaI	GTAC	3 cut(s)	414, 732, 1622
RsaNI	GTAC	3 cut(s)	413, 731, 1621
RseI	CAYNNNNRTG	1 cut(s)	92
SacI	GAGCTC	2 cut(s)	160, 408
SalI	GTCGAC	1 cut(s)	2410
SapI	GCTCTTC	1 cut(s)	903
Sau3AI	GATC	9 cut(s)	66, 235, 633, 922, 1213, 2125, 2454, 2678, 2748
Sau96I	GGNCC	6 cut(s)	356, 860, 1898, 2249, 2542, 2663
SchI	GAGTC	4 cut(s)	740, 967, 1004, 2193
ScrFI	CCNGG	6 cut(s)	660, 858, 1392, 1512, 1640, 1985
SduI	GDGCHC	9 cut(s)	160, 408, 667, 1036, 1508, 1875, 1982, 2489, 2807
SfaNI	GCATC	3 cut(s)	598, 1651, 2500
SfcI	CTRYAG	3 cut(s)	1160, 2337, 2596
SinI	GGWCC	3 cut(s)	1898, 2249, 2663
SmiMI	CAYNNNNRTG	1 cut(s)	92
SmlI	CTYRAG	4 cut(s)	1172, 1793, 1868, 2762
SmoI	CTYRAG	4 cut(s)	1172, 1793, 1868, 2762
SpeI	ACTAGT	1 cut(s)	2774
SphI	GCATGC	1 cut(s)	2571
SseBI	AGGCCT	1 cut(s)	25
SsiI	CCGC	6 cut(s)	2189, 2361, 2559, 2585, 2617, 2645
SspI	AATATT	1 cut(s)	1258
SspMI	CTAG	9 cut(s)	602, 945, 1022, 1229, 2049, 2196, 2682, 2775, 2796
SstI	GAGCTC	2 cut(s)	160, 408
StuI	AGGCCT	1 cut(s)	25
StyD4I	CCNGG	6 cut(s)	658, 856, 1390, 1510, 1638, 1983
StyI	CCWWGG	4 cut(s)	612, 774, 1085, 1781
TaaI	ACNGT	5 cut(s)	310, 1529, 1975, 2071, 2338
TaiI	ACGT	3 cut(s)	807, 2087, 2260
TaqI	TCGA	4 cut(s)	1105, 1468, 2128, 2411
TatI	WGTACW	2 cut(s)	730, 1620
TseFI	GTSAC	4 cut(s)	491, 1315, 1822, 2085
Tsp45I	GTSAC	4 cut(s)	491, 1315, 1822, 2085
TspDTI	ATGAA	7 cut(s)	32, 699, 887, 981, 1193, 2195, 2296
TspGWI	ACGGA	6 cut(s)	833, 1121, 1594, 1883, 2104, 2258
Van91I	CCANNNNNTGG	2 cut(s)	1929, 2258
Vha464I	CTTAAG	2 cut(s)	1172, 2762
VpaK11BI	GGWCC	3 cut(s)	1898, 2249, 2663
VspI	ATTAAT	1 cut(s)	1881
XapI	RAATTY	3 cut(s)	148, 1208, 1297
XbaI	TCTAGA	3 cut(s)	944, 2195, 2681
XceI	RCATGY	3 cut(s)	737, 2571, 2761
XcmI	CCANNNNNNNNNTGG	1 cut(s)	94
XmiI	GTMKAC	1 cut(s)	2411
XmnI	GAANNNNTTC	1 cut(s)	1270
XspI	CTAG	9 cut(s)	602, 945, 1022, 1229, 2049, 2196, 2682, 2775, 2796
ZraI	GACGTC	1 cut(s)	2085
Zsp2I	ATGCAT	1 cut(s)	1006

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.