Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0001061

Physical Location & Seq

Genomic Coordinates Reverse (-)

8113606 .. 8118551

Gene Structure 4946 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1096100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2841 bp

ATGGAGATCTTGCTGATGAACTGGAGGCCTAGCCATGTATGCAAGACAAGAACACTGCTGGCATTGGTCTTTTGCATGTGGGTATCCATGGAAGTGATGGCTGGGACTGAAAATGCCACCCGTTCGTCTGCAAGGCCGAGTACTCTGAATATTGGGGCGCTGTTTACTTTCAATTCAGTTATTGGGAAGGCGGCCAAGCCAGCGATTTTAGCTGCAATCGAGGATGTCAATTCTGATCAGAGTGTTCTTCACGGGACTAAATTGAACGTTATTTTCCATGATACGAATTGCAGCGGATTTCTTGGAACTGTTGAAGCTCTGCAGCTGATTGAAAACGATGTGGTTGCTGCAATTGGCCCACAATCCTCCGGAATAGCTCATGTCATATCCCATGTTGTTAATGAGCTCCATGTACCACTTCTATCATTTGGAGCAACAGACCCCTCGCTTGCTGCTCTCCAGTACCCATATTTTGTCCGAACCACACAGAGTGACTATTTCCAAATGTATGCAGTTGCTGATTTAGTTGAGTATTTTGGATGGAGAGAAGTAATTGCAATCTTTGTAGACGATGATTATGGCAGGAATGGGATTTCTATACTAGGCGATGCCTTGGCGAAGAAGCGTTCCAAGATCTCATACAAGGCTGCCTTCTCTCCTGGAGCCCCCAAAAGTGATATCAATGAATTGTTGGTGGGAGTGAACCTCATGGAATCTCGCGTTTATATTGTTCATGTTAATCCTGACTCTGGTTTAACAATTTTTTCCCTTGCCAAGGCACTTGGAATGATGACCGGTGGCTATGTTTGGATTGCAACCGATTGGCTTCCTTCTCATTTAGATTCTCTAAATCCCCCTGGCCCGGACACAATGAATCTCTTGCAAGGGGTTGTTGCTCTTCGTCATCATACCCCAGATACCGATCTCAAAAAGCGCTTTATGTCTAGATGGAGCAAGCTGAAACATGAAGGTAGTCCAAGCTTCAATTCTTATGCGCTCTATGCATATGACTCTGTTTGGTTAGCTGCCCGTGCGCTTGATGATTTTTTCAACGAAGGTGGGAATGTATCTTTCTCTGATGACCCAAGGTTGAAAGACACAAATAGAAGCACACTGCACTTAACATCACTCCGTATTTTTGATGGAGGCCAAAAATATCTACAGACGATTCTTAAGACGAACTTCACAGGTATAAGTGGTCAGATTGAGTTTGATCAGGAAAAATATCTAGTTCGTCCAGCATACGACATTCTGAATATTGGAGGAACTGGTTCCCGTAGAATTGGCTATTGGTCAAATTCTACTGGTCTCTCAGTCATTGCTCCTGAGATCTTATATAAGAAGCCATTCAATAAGAATAACACTGCTCAACTTTATAGCGTTATATGGCCTGGTGAAGTTACAGCTACACCTCGTGGATGGGTATTTCCCAACAATGGCAGGCCACTTCGAATTGCAGTGCCTTATCGGGTAAGTTACCAAGATTTTGTGGCTAAAGACAACAGCCCTCCAGGTGTCAGAGGATACTGTATTGATGTCTTTGAAGCTGCTGTAAACTTGTTGCCATATGCTGTGCCAAGGACGTATGTGTTGTATGGAAATGGTAAGAGGAATCCTGAGTACAGCGATCTTGTATTCCAGGTTGCGCAAAATAACTTTGATGCAGCTGTTGGAGATGTTACAATTACTACTAATAGGACAAGAATTGTTGATTTTACACAGCCTTACATGGAATCGGGACTTGTTGTAGTTGTTCCTGTCAAACAGGCAAAATCAAAACCTTGGGCTTTCCTCAAGCCATTTACATATCAGATGTGGATGGTCACCGGCGCCTTCTTCCTTTTTGTGGGAGCTGTTGTTTGGATTCTTGAGCACCGGATGAATCACGAGTTCCGTGGTCCACCAAGGCAACAGCTCATAACCATTTTTTGGTTTAGTTTCTCCACAATGTTTTTCTCACATAGAGAGAACACTGTGAGCACCCTGGGACGGCTGGTGCTGGTTATATGGTTGTTTGTAGTGTTAATTATCAATTCCAGCTACACGGCTAGTTTGACTTCAATTCTCACGGTGCAGCAGCTGACATCACGGATTGAAGGGATTAATAGCTTGGTATCAAGTAATGATCCAATCGGAGTTCAAGACGGGTCCTTCATTTGGAAGTATTTGGTTGATGAGCTAAACATAGCAGAATCTAGACTCGTTAAGTTGAAAGACATGGAAGCCTATATTAAAGCCCTTACGGATGGACCAAGACGTGGTGGGGTAGCTGCCATCGTTGATGAGCTTCCTTACATTGAGTTGTTTATGTCCAGCACCAAATGCGCATTCAGGACTGTAGGGCAGGAGTTTACCAAAAGCGGATGGGGATTCGCCTTCCAAAGGGATTCTCCTCTTGCTGTCGACTTGTCGACCGCAATTCTTCAACTTTCAGAGAATGGTGATCTCCAAAAGATACATAATAAGTGGCTTACGCATAACGAGTGCTCTATTCAACTCAATGACGATGAAGACGACCGGCTATCTCTAACAAGCTTTTGGGGCCTGTTTCTTATCTGCGGCATTGCATGCTTCCTTGCTCTTACGGTTTTCTTCTGCAGAATCCTTCTTCAATACCGCAGATTTACCCCAGAGCCTGTGGAAGCGGACGTTGAGGAGATTGGACCTCCAAACACGAGATCTAGACGCTCACTCCGGTCAACTAGTTTCAAGGATCTTATTGATTTTGTAGATAGGAAAGAAACTGAGATCAAGCACATGCTTAAGCGAAAGACTAGTGATAGTAAGGACGAAGCTAGCCCGAGCACTGACCGGCGGCCCCGTTCGCTTTCGGCTGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0004888 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0007154 GO:0007165 GO:0008066 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009507 GO:0009536 GO:0009605 GO:0009611 GO:0009612 GO:0009628 GO:0009719 GO:0009987 GO:0010033 GO:0010034 GO:0010243 GO:0015075 GO:0015085 GO:0015267 GO:0015276 GO:0015318 GO:0016020 GO:0019722 GO:0019932 GO:0022803 GO:0022834 GO:0022836 GO:0022838 GO:0022839 GO:0022857 GO:0022890 GO:0023052 GO:0030001 GO:0033554 GO:0034220 GO:0035556 GO:0038023 GO:0042221 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046677 GO:0046873 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055085 GO:0060089 GO:0065007 GO:0070417 GO:0070588 GO:0070838 GO:0070887 GO:0071214 GO:0071229 GO:0071230 GO:0071236 GO:0071260 GO:0071310 GO:0071311 GO:0071417 GO:0071495 GO:0071496 GO:0071944 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:0104004 GO:1901698 GO:1901699 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

947

Amino Acids

105.95

Weight (kDa)

7.02

Isoelectric Point (pI)

41.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peripla_BP_6	PF13458	50 - 347	1.1e-20	Periplasmic binding protein
ANF_receptor	PF01094	65 - 422	1.5e-91	Receptor family ligand binding region
Lig_chan-Glu_bd	PF10613	488 - 587	1.9e-11	Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3	PF00497	541 - 825	2.2e-11	Bacterial extracellular solute-binding proteins, family 3
Lig_chan	PF00060	603 - 857	1.6e-39	Ligand-gated ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0001061)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G05200 AT1G05200 AT1G05200 AT1G05200 AT1G05200 AT1G05200 AT1G05200 AT2G32390 AT2G32390 AT2G32390 AT2G32390 AT2G32390 AT2G32390 AT2G32390
fragaria_vesca	FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291
malus_domestica	MD09G1069900.v1.1 MD09G1070100.v1.1 MD09G1107700.v1.1 MD09G1108100.v1.1 MD17G1096100.v1.1
prunus_persica	Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1
pyrus_communis	pycom09g03000 pycom09g03030 pycom09g03050 pycom09g03070 pycom15g20430 pycom17g09220
rosa_chinensis	RchiOBHm_Chr1g0324131 RchiOBHm_Chr2g0159571 RchiOBHm_Chr2g0159581
rosa_laevigata	RLG00000021167
rosa_multiflora	Rmu_co8397097.1_g000001 Rmu_sc0004966.1_g000017 Rmu_sc0020031.1_g000001
rosa_roxburghii	Rroxscaffold_2G00090390
rosa_rugosa	Rorug02G0476900 Rorug02G0477000 Rorug02G0477100 Rorug02G0477200
rosa_samantha	Rh2AG544200 Rh2BG557000 Rh2CG527200 Rh2DG566000
rosa_wichuraiana	Rw2G044990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	2 cut(s)	1647, 2326
AccB1I	GGYRCC	1 cut(s)	1829
AccB7I	CCANNNNNTGG	2 cut(s)	1929, 2258
AccI	GTMKAC	3 cut(s)	567, 2403, 2411
AccII	CGCG	1 cut(s)	720
AccIII	TCCGGA	1 cut(s)	368
AciI	CCGC	8 cut(s)	191, 294, 2361, 2415, 2559, 2617, 2645, 2815
AclI	AACGTT	1 cut(s)	267
AclWI	GGATC	2 cut(s)	2120, 2721
AcoI	YGGCCR	1 cut(s)	192
AcsI	RAATTY	1 cut(s)	1297
AcyI	GRCGYC	1 cut(s)	1830
AdeI	CACNNNGTG	2 cut(s)	491, 1415
AfaI	GTAC	4 cut(s)	142, 414, 464, 1622
AfeI	AGCGCT	1 cut(s)	935
AflII	CTTAAG	2 cut(s)	1172, 2762
AgeI	ACCGGT	1 cut(s)	794
AhlI	ACTAGT	2 cut(s)	2702, 2774
AjiI	CACGTC	1 cut(s)	2258
AjnI	CCWGG	6 cut(s)	658, 856, 1390, 1510, 1638, 1983
AjuI	GAANNNNNNNTTGG	6 cut(s)	970, 1002, 1273, 1305, 2312, 2344
Alw21I	GWGCWC	5 cut(s)	408, 1875, 1982, 2489, 2807
Alw26I	GTCTC	1 cut(s)	1313
AlwI	GGATC	2 cut(s)	2120, 2721
AlwNI	CAGNNNCTG	3 cut(s)	518, 2080, 2636
Ama87I	CYCGRG	1 cut(s)	2800
Aor13HI	TCCGGA	1 cut(s)	368
Aor51HI	AGCGCT	1 cut(s)	935
ApoI	RAATTY	1 cut(s)	1297
AseI	ATTAAT	1 cut(s)	2103
AsiGI	ACCGGT	1 cut(s)	794
Asp700I	GAANNNNTTC	2 cut(s)	1270, 1346
AspLEI	GCGC	7 cut(s)	160, 936, 997, 1036, 1648, 1832, 2327
AspS9I	GGNCC	8 cut(s)	356, 860, 1898, 2148, 2249, 2542, 2663, 2817
AsuC2I	CCSGG	1 cut(s)	863
AsuHPI	GGTGA	3 cut(s)	1406, 1816, 2453
AsuII	TTCGAA	1 cut(s)	1450
AsuNHI	GCTAGC	1 cut(s)	2795
AvaI	CYCGRG	1 cut(s)	2800
AvaII	GGWCC	4 cut(s)	1898, 2148, 2249, 2663
BanI	GGYRCC	1 cut(s)	1829
BanII	GRGCYC	2 cut(s)	408, 667
BauI	CACGAG	3 cut(s)	1413, 1886, 2674
BbsI	GAAGAC	1 cut(s)	2517
Bbv12I	GWGCWC	5 cut(s)	408, 1875, 1982, 2489, 2807
BccI	CCATC	9 cut(s)	91, 534, 942, 1136, 1413, 1813, 2240, 2282, 2358
BceAI	ACGGC	2 cut(s)	2006, 2061
BcgI	CGANNNNNNTGC	2 cut(s)	326, 360
BciT130I	CCWGG	6 cut(s)	660, 858, 1392, 1512, 1640, 1985
BciVI	GTATCC	2 cut(s)	94, 1517
BclI	TGATCA	2 cut(s)	235, 1213
BcnI	CCSGG	1 cut(s)	863
BcoDI	GTCTC	1 cut(s)	1313
BcuI	ACTAGT	2 cut(s)	2702, 2774
BfmI	CTRYAG	4 cut(s)	320, 1160, 2337, 2596
BfoI	RGCGCY	3 cut(s)	161, 937, 1833
BfrI	CTTAAG	2 cut(s)	1172, 2762
BfuI	GTATCC	2 cut(s)	94, 1517
BglII	AGATCT	4 cut(s)	6, 633, 1329, 2678
BmcAI	AGTACT	1 cut(s)	142
Bme1390I	CCNGG	7 cut(s)	660, 858, 863, 1392, 1512, 1640, 1985
Bme18I	GGWCC	4 cut(s)	1898, 2148, 2249, 2663
BmeT110I	CYCGRG	1 cut(s)	2800
BmgBI	CACGTC	1 cut(s)	2258
BmgT120I	GGNCC	8 cut(s)	356, 860, 1898, 2148, 2249, 2542, 2663, 2817
BmiI	GGNNCC	6 cut(s)	664, 1273, 1831, 2149, 2543, 2819
BmrFI	CCNGG	7 cut(s)	660, 858, 863, 1392, 1512, 1640, 1985
BmsI	GCATC	2 cut(s)	598, 1651
BmtI	GCTAGC	1 cut(s)	2799
BpiI	GAAGAC	1 cut(s)	2517
BplI	GAGNNNNNCTC	2 cut(s)	690, 722
BpmI	CTGGAG	4 cut(s)	43, 443, 681, 1494
Bpu14I	TTCGAA	1 cut(s)	1450
BpuEI	CTTGAG	2 cut(s)	1778, 1889
BpuMI	CCSGG	1 cut(s)	863
BsaBI	GATNNNNATC	1 cut(s)	921
BsaHI	GRCGYC	1 cut(s)	1830
BsaI	GGTCTC	1 cut(s)	1313
BsaWI	WCCGGW	4 cut(s)	368, 794, 1875, 2694
BsaXI	ACNNNNNCTCC	4 cut(s)	1842, 1872, 2676, 2706
Bse118I	RCCGGY	4 cut(s)	794, 1826, 2517, 2811
Bse1I	ACTGG	4 cut(s)	26, 460, 1273, 1309
Bse3DI	GCAATG	2 cut(s)	1317, 2562
Bse8I	GATNNNNATC	1 cut(s)	921
BseAI	TCCGGA	1 cut(s)	368
BseBI	CCWGG	6 cut(s)	660, 858, 1392, 1512, 1640, 1985
BseGI	GGATG	7 cut(s)	229, 545, 1424, 1824, 1884, 2251, 2369
BseJI	GATNNNNATC	1 cut(s)	921
BseMI	GCAATG	2 cut(s)	1317, 2562
BseMII	CTCAG	4 cut(s)	1317, 1326, 1608, 2736
BseNI	ACTGG	4 cut(s)	26, 460, 1273, 1309
BseRI	GAGGAG	2 cut(s)	2382, 2669
BseYI	CCCAGC	1 cut(s)	101
BsgI	GTGCAG	2 cut(s)	1100, 2093
Bsh1236I	CGCG	1 cut(s)	720
Bsh1285I	CGRYCG	2 cut(s)	2415, 2518
BshNI	GGYRCC	1 cut(s)	1829
BshTI	ACCGGT	1 cut(s)	794
BsiEI	CGRYCG	2 cut(s)	2415, 2518
BsiHKAI	GWGCWC	5 cut(s)	408, 1875, 1982, 2489, 2807
BsiHKCI	CYCGRG	1 cut(s)	2800
BsiSI	CCGG	8 cut(s)	369, 795, 863, 1827, 1876, 2518, 2695, 2812
BslFI	GGGAC	4 cut(s)	118, 268, 1752, 2001
BsmAI	GTCTC	1 cut(s)	1313
BsmFI	GGGAC	4 cut(s)	118, 268, 1752, 2001
BsmI	GAATGC	1 cut(s)	2327
Bso31I	GGTCTC	1 cut(s)	1313
BsoBI	CYCGRG	1 cut(s)	2800
Bsp119I	TTCGAA	1 cut(s)	1450
Bsp1286I	GDGCHC	6 cut(s)	408, 667, 1875, 1982, 2489, 2807
Bsp13I	TCCGGA	1 cut(s)	368
Bsp19I	CCATGG	1 cut(s)	87
BspACI	CCGC	8 cut(s)	191, 294, 2361, 2415, 2559, 2617, 2645, 2815
BspCNI	CTCAG	4 cut(s)	1318, 1325, 1609, 2737
BspEI	TCCGGA	1 cut(s)	368
BspFNI	CGCG	1 cut(s)	720
BspLI	GGNNCC	6 cut(s)	664, 1273, 1831, 2149, 2543, 2819
BspMAI	CTGCAG	2 cut(s)	324, 2600
BspOI	GCTAGC	1 cut(s)	2799
BspPI	GGATC	2 cut(s)	2120, 2721
BspQI	GCTCTTC	1 cut(s)	903
BspT104I	TTCGAA	1 cut(s)	1450
BspT107I	GGYRCC	1 cut(s)	1829
BspTI	CTTAAG	2 cut(s)	1172, 2762
BspTNI	GGTCTC	1 cut(s)	1313
BsrDI	GCAATG	2 cut(s)	1317, 2562
BsrFI	RCCGGY	4 cut(s)	794, 1826, 2517, 2811
BsrI	ACTGG	4 cut(s)	26, 460, 1273, 1309
BssAI	RCCGGY	4 cut(s)	794, 1826, 2517, 2811
BssNI	GRCGYC	1 cut(s)	1830
BssSI	CACGAG	3 cut(s)	1413, 1886, 2674
BssT1I	CCWWGG	7 cut(s)	87, 612, 774, 1085, 1577, 1781, 1904
Bst2BI	CACGAG	3 cut(s)	1413, 1886, 2674
Bst2UI	CCWGG	6 cut(s)	660, 858, 1392, 1512, 1640, 1985
Bst4CI	ACNGT	6 cut(s)	310, 1529, 1975, 2071, 2338, 2587
Bst6I	CTCTTC	1 cut(s)	903
BstACI	GRCGYC	1 cut(s)	1830
BstAFI	CTTAAG	2 cut(s)	1172, 2762
BstAPI	GCANNNNNTGC	2 cut(s)	2322, 2568
BstBI	TTCGAA	1 cut(s)	1450
BstC8I	GCNNGC	7 cut(s)	60, 201, 450, 956, 1442, 2569, 2797
BstDEI	CTNAG	4 cut(s)	1312, 1326, 1617, 2745
BstDSI	CCRYGG	2 cut(s)	87, 1894
BstEII	GGTNACC	1 cut(s)	1822
BstENI	CCTNNNNNAGG	2 cut(s)	773, 2380
BstF5I	GGATG	7 cut(s)	229, 545, 1424, 1824, 1884, 2251, 2369
BstFNI	CGCG	1 cut(s)	720
BstH2I	RGCGCY	3 cut(s)	161, 937, 1833
BstHHI	GCGC	7 cut(s)	160, 936, 997, 1036, 1648, 1832, 2327
BstMAI	GTCTC	1 cut(s)	1313
BstMCI	CGRYCG	2 cut(s)	2415, 2518
BstMWI	GCNNNNNNNGC	9 cut(s)	39, 200, 209, 1001, 1031, 2322, 2541, 2568, 2824
BstNI	CCWGG	6 cut(s)	660, 858, 1392, 1512, 1640, 1985
BstNSI	RCATGY	3 cut(s)	79, 2571, 2761
BstPI	GGTNACC	1 cut(s)	1822
BstSCI	CCNGG	7 cut(s)	658, 856, 861, 1390, 1510, 1638, 1983
BstSFI	CTRYAG	4 cut(s)	320, 1160, 2337, 2596
BstUI	CGCG	1 cut(s)	720
BstV2I	GAAGAC	1 cut(s)	2517
BstX2I	RGATCY	5 cut(s)	6, 633, 1329, 2678, 2713
BstYI	RGATCY	5 cut(s)	6, 633, 1329, 2678, 2713
BsuI	GTATCC	2 cut(s)	94, 1517
BtgI	CCRYGG	2 cut(s)	87, 1894
BtgZI	GCGATG	1 cut(s)	621
BtrI	CACGTC	1 cut(s)	2258
BtsCI	GGATG	7 cut(s)	229, 545, 1424, 1824, 1884, 2251, 2369
BtsI	GCAGTG	4 cut(s)	53, 1112, 1362, 1464
BtsIMutI	CAGTG	6 cut(s)	53, 1112, 1362, 1464, 1971, 2805
Cac8I	GCNNGC	7 cut(s)	60, 201, 450, 956, 1442, 2569, 2797
CaiI	CAGNNNCTG	3 cut(s)	518, 2080, 2636
CfoI	GCGC	7 cut(s)	160, 936, 997, 1036, 1648, 1832, 2327
Cfr10I	RCCGGY	4 cut(s)	794, 1826, 2517, 2811
Cfr13I	GGNCC	8 cut(s)	356, 860, 1898, 2148, 2249, 2542, 2663, 2817
CseI	GACGC	1 cut(s)	2694
Csp6I	GTAC	4 cut(s)	141, 413, 463, 1621
CspAI	ACCGGT	1 cut(s)	794
CspCI	CAANNNNNGTGG	2 cut(s)	1039, 1074
CviQI	GTAC	4 cut(s)	141, 413, 463, 1621
DdeI	CTNAG	4 cut(s)	1312, 1326, 1617, 2745
DinI	GGCGCC	1 cut(s)	1831
DraIII	CACNNNGTG	2 cut(s)	491, 1415
EaeI	YGGCCR	1 cut(s)	192
Eam1104I	CTCTTC	1 cut(s)	903
EarI	CTCTTC	1 cut(s)	903
Ecl136II	GAGCTC	1 cut(s)	406
Eco130I	CCWWGG	7 cut(s)	87, 612, 774, 1085, 1577, 1781, 1904
Eco147I	AGGCCT	1 cut(s)	28
Eco24I	GRGCYC	2 cut(s)	408, 667
Eco31I	GGTCTC	1 cut(s)	1313
Eco32I	GATATC	1 cut(s)	679
Eco47I	GGWCC	4 cut(s)	1898, 2148, 2249, 2663
Eco47III	AGCGCT	1 cut(s)	935
Eco53kI	GAGCTC	1 cut(s)	406
Eco88I	CYCGRG	1 cut(s)	2800
Eco91I	GGTNACC	1 cut(s)	1822
EcoICRI	GAGCTC	1 cut(s)	406
EcoNI	CCTNNNNNAGG	2 cut(s)	773, 2380
EcoO109I	RGGNCCY	2 cut(s)	2148, 2542
EcoO65I	GGTNACC	1 cut(s)	1822
EcoRII	CCWGG	6 cut(s)	658, 856, 1390, 1510, 1638, 1983
EcoRV	GATATC	1 cut(s)	679
EcoT14I	CCWWGG	7 cut(s)	87, 612, 774, 1085, 1577, 1781, 1904
EcoT22I	ATGCAT	1 cut(s)	1006
EcoT38I	GRGCYC	2 cut(s)	408, 667
EgeI	GGCGCC	1 cut(s)	1831
EheI	GGCGCC	1 cut(s)	1831
ErhI	CCWWGG	7 cut(s)	87, 612, 774, 1085, 1577, 1781, 1904
FalI	AAGNNNNNCTT	4 cut(s)	635, 667, 1166, 1198
FaqI	GGGAC	4 cut(s)	118, 268, 1752, 2001
FauNDI	CATATG	2 cut(s)	1006, 1567
FbaI	TGATCA	2 cut(s)	235, 1213
FblI	GTMKAC	3 cut(s)	567, 2403, 2411
FokI	GGATG	7 cut(s)	236, 552, 1431, 1831, 1891, 2258, 2376
FriOI	GRGCYC	2 cut(s)	408, 667
FspAI	RTGCGCAY	1 cut(s)	2326
FspI	TGCGCA	2 cut(s)	1647, 2326
GlaI	GCGC	7 cut(s)	159, 935, 996, 1035, 1647, 1831, 2326
GsaI	CCCAGC	1 cut(s)	105
GsuI	CTGGAG	4 cut(s)	43, 443, 681, 1494
HaeII	RGCGCY	3 cut(s)	161, 937, 1833
HapII	CCGG	8 cut(s)	369, 795, 863, 1827, 1876, 2518, 2695, 2812
HgaI	GACGC	1 cut(s)	2694
HhaI	GCGC	7 cut(s)	160, 936, 997, 1036, 1648, 1832, 2327
Hin1I	GRCGYC	1 cut(s)	1830
Hin6I	GCGC	7 cut(s)	158, 934, 995, 1034, 1646, 1830, 2325
HinP1I	GCGC	7 cut(s)	158, 934, 995, 1034, 1646, 1830, 2325
HincII	GTYRAC	3 cut(s)	2404, 2412, 2700
HindII	GTYRAC	3 cut(s)	2404, 2412, 2700
HindIII	AAGCTT	2 cut(s)	979, 2533
HpaII	CCGG	8 cut(s)	369, 795, 863, 1827, 1876, 2518, 2695, 2812
HphI	GGTGA	3 cut(s)	1406, 1816, 2453
Hpy166II	GTNNAC	9 cut(s)	165, 568, 703, 1555, 1901, 2352, 2404, 2412, 2700
Hpy8I	GTNNAC	9 cut(s)	165, 568, 703, 1555, 1901, 2352, 2404, 2412, 2700
HpyCH4III	ACNGT	6 cut(s)	310, 1529, 1975, 2071, 2338, 2587
HpyCH4IV	ACGT	4 cut(s)	267, 1583, 2257, 2649
HpyF10VI	GCNNNNNNNGC	9 cut(s)	39, 200, 209, 1001, 1031, 2322, 2541, 2568, 2824
HpyF3I	CTNAG	4 cut(s)	1312, 1326, 1617, 2745
HpySE526I	ACGT	4 cut(s)	267, 1583, 2257, 2649
Hsp92I	GRCGYC	1 cut(s)	1830
HspAI	GCGC	7 cut(s)	158, 934, 995, 1034, 1646, 1830, 2325
KasI	GGCGCC	1 cut(s)	1829
Kpn2I	TCCGGA	1 cut(s)	368
Ksp22I	TGATCA	2 cut(s)	235, 1213
LguI	GCTCTTC	1 cut(s)	903
LmnI	GCTCC	6 cut(s)	411, 431, 662, 951, 1327, 1850
LweI	GCATC	2 cut(s)	598, 1651
MaeII	ACGT	4 cut(s)	267, 1583, 2257, 2649
MaeIII	GTNAC	5 cut(s)	491, 1399, 1475, 1678, 1822
MboII	GAAGA	8 cut(s)	239, 631, 890, 1828, 2414, 2522, 2584, 2600
MfeI	CAATTG	1 cut(s)	351
MflI	RGATCY	5 cut(s)	6, 633, 1329, 2678, 2713
MhlI	GDGCHC	6 cut(s)	408, 667, 1875, 1982, 2489, 2807
Mly113I	GGCGCC	1 cut(s)	1830
MlyI	GAGTC	3 cut(s)	740, 1004, 2193
MmeI	TCCRAC	1 cut(s)	1651
Mph1103I	ATGCAT	1 cut(s)	1006
MroI	TCCGGA	1 cut(s)	368
MroXI	GAANNNNTTC	2 cut(s)	1270, 1346
MslI	CAYNNNNRTG	1 cut(s)	92
MspA1I	CMGCKG	4 cut(s)	294, 325, 1667, 2080
MspCI	CTTAAG	2 cut(s)	1172, 2762
MspI	CCGG	8 cut(s)	369, 795, 863, 1827, 1876, 2518, 2695, 2812
MspR9I	CCNGG	7 cut(s)	660, 858, 863, 1392, 1512, 1640, 1985
MunI	CAATTG	1 cut(s)	351
Mva1269I	GAATGC	1 cut(s)	2327
MvaI	CCWGG	6 cut(s)	660, 858, 1392, 1512, 1640, 1985
MvnI	CGCG	1 cut(s)	720
MwoI	GCNNNNNNNGC	9 cut(s)	39, 200, 209, 1001, 1031, 2322, 2541, 2568, 2824
NarI	GGCGCC	1 cut(s)	1830
NciI	CCSGG	1 cut(s)	863
NcoI	CCATGG	1 cut(s)	87
NdeI	CATATG	2 cut(s)	1006, 1567
NheI	GCTAGC	1 cut(s)	2795
NlaIV	GGNNCC	6 cut(s)	664, 1273, 1831, 2149, 2543, 2819
NmeAIII	GCCGAG	1 cut(s)	162
NmuCI	GTSAC	2 cut(s)	491, 1822
NsbI	TGCGCA	2 cut(s)	1647, 2326
NsiI	ATGCAT	1 cut(s)	1006
NspI	RCATGY	3 cut(s)	79, 2571, 2761
NspV	TTCGAA	1 cut(s)	1450
PaeI	GCATGC	1 cut(s)	2571
PasI	CCCWGGG	1 cut(s)	1984
PceI	AGGCCT	1 cut(s)	28
PciSI	GCTCTTC	1 cut(s)	903
PcsI	WCGNNNNNNNCGW	1 cut(s)	2511
PctI	GAATGC	1 cut(s)	2327
PdmI	GAANNNNTTC	2 cut(s)	1270, 1346
PflMI	CCANNNNNTGG	2 cut(s)	1929, 2258
PfoI	TCCNGGA	1 cut(s)	658
PinAI	ACCGGT	1 cut(s)	794
PleI	GAGTC	3 cut(s)	740, 1004, 2193
PluTI	GGCGCC	1 cut(s)	1833
PpsI	GAGTC	3 cut(s)	740, 1004, 2193
PpuMI	RGGWCCY	1 cut(s)	2148
PshBI	ATTAAT	1 cut(s)	2103
Psp124BI	GAGCTC	1 cut(s)	408
Psp1406I	AACGTT	1 cut(s)	267
Psp5II	RGGWCCY	1 cut(s)	2148
Psp6I	CCWGG	6 cut(s)	658, 856, 1390, 1510, 1638, 1983
PspEI	GGTNACC	1 cut(s)	1822
PspFI	CCCAGC	1 cut(s)	101
PspGI	CCWGG	6 cut(s)	658, 856, 1390, 1510, 1638, 1983
PspN4I	GGNNCC	6 cut(s)	664, 1273, 1831, 2149, 2543, 2819
PspPI	GGNCC	8 cut(s)	356, 860, 1898, 2148, 2249, 2542, 2663, 2817
PspPPI	RGGWCCY	1 cut(s)	2148
PstI	CTGCAG	2 cut(s)	324, 2600
PstNI	CAGNNNCTG	3 cut(s)	518, 2080, 2636
PsuI	RGATCY	5 cut(s)	6, 633, 1329, 2678, 2713
PvuII	CAGCTG	3 cut(s)	325, 1667, 2080
RsaI	GTAC	4 cut(s)	142, 414, 464, 1622
RsaNI	GTAC	4 cut(s)	141, 413, 463, 1621
RseI	CAYNNNNRTG	1 cut(s)	92
SacI	GAGCTC	1 cut(s)	408
SalI	GTCGAC	2 cut(s)	2402, 2410
SapI	GCTCTTC	1 cut(s)	903
Sau96I	GGNCC	8 cut(s)	356, 860, 1898, 2148, 2249, 2542, 2663, 2817
ScaI	AGTACT	1 cut(s)	142
SchI	GAGTC	3 cut(s)	740, 1004, 2193
ScrFI	CCNGG	7 cut(s)	660, 858, 863, 1392, 1512, 1640, 1985
SduI	GDGCHC	6 cut(s)	408, 667, 1875, 1982, 2489, 2807
SfaNI	GCATC	2 cut(s)	598, 1651
SfcI	CTRYAG	4 cut(s)	320, 1160, 2337, 2596
SfoI	GGCGCC	1 cut(s)	1831
SfuI	TTCGAA	1 cut(s)	1450
SgrAI	CRCCGGYG	1 cut(s)	1826
SinI	GGWCC	4 cut(s)	1898, 2148, 2249, 2663
SmiMI	CAYNNNNRTG	1 cut(s)	92
SmlI	CTYRAG	4 cut(s)	1172, 1793, 1868, 2762
SmoI	CTYRAG	4 cut(s)	1172, 1793, 1868, 2762
SpeI	ACTAGT	2 cut(s)	2702, 2774
SphI	GCATGC	1 cut(s)	2571
SseBI	AGGCCT	1 cut(s)	28
SsiI	CCGC	8 cut(s)	191, 294, 2361, 2415, 2559, 2617, 2645, 2815
SspDI	GGCGCC	1 cut(s)	1829
SspI	AATATT	2 cut(s)	151, 1258
SstI	GAGCTC	1 cut(s)	408
StuI	AGGCCT	1 cut(s)	28
StyD4I	CCNGG	7 cut(s)	658, 856, 861, 1390, 1510, 1638, 1983
StyI	CCWWGG	7 cut(s)	87, 612, 774, 1085, 1577, 1781, 1904
TaaI	ACNGT	6 cut(s)	310, 1529, 1975, 2071, 2338, 2587
TaiI	ACGT	4 cut(s)	270, 1586, 2260, 2652
TaqI	TCGA	4 cut(s)	219, 1450, 2403, 2411
TatI	WGTACW	2 cut(s)	140, 1620
TauI	GCSGC	3 cut(s)	194, 2562, 2818
TscAI	CASTG	6 cut(s)	60, 1119, 1369, 1464, 1978, 2812
TseFI	GTSAC	2 cut(s)	491, 1822
Tsp45I	GTSAC	2 cut(s)	491, 1822
TspDTI	ATGAA	8 cut(s)	32, 699, 722, 887, 981, 1895, 2143, 2523
TspGWI	ACGGA	4 cut(s)	1121, 1883, 2104, 2258
TspRI	CASTG	6 cut(s)	60, 1119, 1369, 1464, 1978, 2812
Van91I	CCANNNNNTGG	2 cut(s)	1929, 2258
Vha464I	CTTAAG	2 cut(s)	1172, 2762
VpaK11BI	GGWCC	4 cut(s)	1898, 2148, 2249, 2663
VspI	ATTAAT	1 cut(s)	2103
XagI	CCTNNNNNAGG	2 cut(s)	773, 2380
XapI	RAATTY	1 cut(s)	1297
XbaI	TCTAGA	3 cut(s)	944, 2195, 2681
XceI	RCATGY	3 cut(s)	79, 2571, 2761
XcmI	CCANNNNNNNNNTGG	1 cut(s)	94
XmiI	GTMKAC	3 cut(s)	567, 2403, 2411
XmnI	GAANNNNTTC	2 cut(s)	1270, 1346
ZrmI	AGTACT	1 cut(s)	142
Zsp2I	ATGCAT	1 cut(s)	1006

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.