Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr9

Orthogroup ID

OG0001061

Physical Location & Seq

Genomic Coordinates Reverse (-)

2210018 .. 2218970

Gene Structure 8953 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom09g03000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2859 bp

ATGGAGGTCTTGCTGATGAACAGGCCTACTGGCCATGTATGCAAGACAAGAACACTGCTTACATTGATCTTGTGCATGTGGGTGTCCATGCAAGTGATGGCTGGGACAGAAAATGCTACGCATTCGTCTGCAAGGCCGAGTTCTCTGAATATTGGAGCTCTGTTTACATTCAATTCGGTTATTGGGAGGGCAGCCAAACCAGCAATTTTAGCTGCAATTGATGATGTCAATTCTGATCAAAGCGTTCTTCGCGGGACAAAATTGAAGATTATTCTCCATGACACGAATTGCAGTGGATTTCTTGGAACTGTTGAAGCTCTGGAGCTGATTGAAAAAGATGTGGTTGCTGCAATCGGCCCACAATCCTCCGGAATAGCTCATGTCATATCCCATGTTGTTAATGAGCTCCATGTACCACTTCTATCATTTGGAGCAACAGACCCCTCCCTTGCTGCTCTGCAATACCCATATTTTGTCCGAACCACACAGAGTGACTTATTTCAAATGCATGCAGTTGCTGATTTAGTTGAGTATTTTGGATGGAGAGAAGTAATAGCAATCTTTGTAAATGATGATTATGGAAGAAATGGGATTTCTATACTAGGCGATGCCTTGGCGACGAAGCGTTCTAAGATCTCATACAAGGCTGCCTTCTCTCCTGGAGCCCCCAAAAGCGATATCAATGAGTTGTTGGTGGGAGTGAACCTTATGGAATCTCGGGTTTATATTGTACATGTTAATCCTGACTCTGGTTTAACAATTTTTTCCATTGCCAAGGCACTTGGAATGATGACGAGTGGCTATGTCTGGATTGCAACGGATTGGCTTCCTTCTCATTTAGATTCACTAAATTCCCCTGGCCCCGACACAATGAATCTCTTACAAGGGGTTGTTGCTCTTCGTCATCATACCCCAGATACTGATCTCAAAAAGCGCTTTATGTCTAGATGGAGCAAATTGAAACATGAAGGGACTCCAAGCTTCAATTCTTATGCGCTCTATGCATACGACTCTATTTGGCTAGCAGCCCGTGCCCTTGATGTTTTCTTCAATGAAGGTGGGAATGTATCTTTCTCTAATGACCCAAGGTTGAAAGACACAAATCGAAGCACACTGCATTTAACATCACTCCGTATTTTTGATGGAGGCCAAAAATACCTACAGACGATTCTTAAGATGAACTTCACAGGTATAAGTGGTCAGATTGAGTTTGATCAAGATAAATATCTAGTTCGTCCAGCTTATGACATTCTGAATATTGGAGGAACTGGTTCCCGTAGAGTTGGCTATTGGTCAAATTCTAGTGGTCTCTCAGTCATTGCTCCAGAGAGATTATATAAGAAGCCATCCAATAGGAATACCACTGCTCAACTTTATAGCGTTATATGGCCTGGTGAAGTCACTGCTATACCCCGGGGATGGGTATTTCCCAACAATGGCAGGCCACTGCGAATTGGAGTGCCTTATCGAGTAAGTTACAAAGAATTTGTGGCTAAAGACAAGAGCCCTCCAGGGGTCAGAGGATACTGTATTGATGTCTTTGAAGCTGCTGTAAACTTGTTGCCGTATGCTGTGCCACGGACGTATATGTTGTATGGAGATGGTAAGAGGAATCCTGAGTACAGCAGTCTTGTAGCCCAGGTTGCGCAAAATAACTTTGATGCAGCTGTTGGAGATGTTACAATTACTACTAATAGGACAAGAATAGTTGATTTTACGCAGCCTTACATGGAATCGGGACTTGTTGTAGTTGTTCCTGTCAAACAGGCAAAATCAAACCCTTGGGCTTTCCTCAAGCCATTTACTTATCAGATGTGGTTGGTCACAGGTGCCTTCTTCCTTTTAGTGGGAGCTGTTGTTTGGATTCTTGAGCACCGGATGAATCATGAGTTCCGTGGTCCACCGAGCCAACAGCTCATGACCATTTTTTGGTTTAGTTTCTCCACAATGTTTTTCTCACACAGAGAGAACACTGTGAGCACCCTGGGACGGCTGGTGCTGATTATATGGTTATTTGTAGTGTTAATTATCAATTCCAGCTACACGGCTAGTTTGACTTCAATTCTCACGGTGCAACAGCTGACATCACGGATTGAAGGGATTGACAGCTTGATATCAAGTAATGATCGAATCGGAGTTCAAGACGGGTCATTCGCTTGGAGGTATTTGGTTGATGAGATGAACATAGCAGAATCTAGACTCGTTAAGTTGAAAGACATGGAAACCTATTTTAAAGCCCTCACAGATGGACCAAGACGTGGTGGGGTAGCTGCCATCGTTGATGAGCTTCCTTACATTGAGTTGTTCATGTCCAACACCAAATGCACATTCAGGACCGTAGGGCAGGAGTTTACCAAAAGCGGATGGGGATTCGCATTCCAAAGGGATTCTCCTCTTGCTGTTGACTTGTCGACTGCGATTCTTCAACTCTCAGAGAACGGTGATCTACAAAAGATCCATAATAAGTGGCTTACGCACAGTGAGTGCTCTACTCAACTCACTGAAATTGATGCAGACCAACTATCTCTAACAAGCTTTTGGGGCCTGTTTCTTATCTGCGGTATTGCATGCTTCCTTGCTCTTGCGGTGTTCTTCTGTAGAATCCTTTGTCAATACCGCAGATTTACCCCAGAGCCCGTGGAAGCAGACACTGAGGAGAGTGGACCTACCAACCCGAGATCTAGACGCTCACTTGGGTCAACCAGTTTCAAGGGTCTTATGGTCTTTGTAGATAAGAAAGAAGCGGAGATCAAGCACATGCTTAAGCGAAAGACTAGTGATAGTAAGCACGAAGCTAGCCCGAGCGCTGATGGGCAGCTGCATTCCCTTCGTGAGGAAGATTTGCTTTCGGCTGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0004888 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0007154 GO:0007165 GO:0008066 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009507 GO:0009536 GO:0009605 GO:0009611 GO:0009612 GO:0009628 GO:0009719 GO:0009987 GO:0010033 GO:0010034 GO:0010243 GO:0015075 GO:0015085 GO:0015267 GO:0015276 GO:0015318 GO:0016020 GO:0019722 GO:0019932 GO:0022803 GO:0022834 GO:0022836 GO:0022838 GO:0022839 GO:0022857 GO:0022890 GO:0023052 GO:0030001 GO:0033554 GO:0034220 GO:0035556 GO:0038023 GO:0042221 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046677 GO:0046873 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055085 GO:0060089 GO:0065007 GO:0070417 GO:0070588 GO:0070838 GO:0070887 GO:0071214 GO:0071229 GO:0071230 GO:0071236 GO:0071260 GO:0071310 GO:0071311 GO:0071417 GO:0071495 GO:0071496 GO:0071944 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:0104004 GO:1901698 GO:1901699 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

953

Amino Acids

106.02

Weight (kDa)

7.92

Isoelectric Point (pI)

39.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peripla_BP_6	PF13458	51 - 346	3.9e-18	Periplasmic binding protein
ANF_receptor	PF01094	65 - 422	1.2e-90	Receptor family ligand binding region
Lig_chan-Glu_bd	PF10613	488 - 587	8.4e-12	Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3	PF00497	540 - 825	3.5e-12	Bacterial extracellular solute-binding proteins, family 3
Lig_chan	PF00060	603 - 857	2.4e-39	Ligand-gated ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001061)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G05200 AT1G05200 AT1G05200 AT1G05200 AT1G05200 AT1G05200 AT1G05200 AT2G32390 AT2G32390 AT2G32390 AT2G32390 AT2G32390 AT2G32390 AT2G32390
fragaria_vesca	FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291
malus_domestica	MD09G1069900.v1.1 MD09G1070100.v1.1 MD09G1107700.v1.1 MD09G1108100.v1.1 MD17G1096100.v1.1
prunus_persica	Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1
pyrus_communis	pycom09g03000 pycom09g03030 pycom09g03050 pycom09g03070 pycom15g20430 pycom17g09220
rosa_chinensis	RchiOBHm_Chr1g0324131 RchiOBHm_Chr2g0159571 RchiOBHm_Chr2g0159581
rosa_laevigata	RLG00000021167
rosa_multiflora	Rmu_co8397097.1_g000001 Rmu_sc0004966.1_g000017 Rmu_sc0020031.1_g000001
rosa_roxburghii	Rroxscaffold_2G00090390
rosa_rugosa	Rorug02G0476900 Rorug02G0477000 Rorug02G0477100 Rorug02G0477200
rosa_samantha	Rh2AG544200 Rh2BG557000 Rh2CG527200 Rh2DG566000
rosa_wichuraiana	Rw2G044990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1647
AccB1I	GGYRCC	1 cut(s)	1829
AccB7I	CCANNNNNTGG	2 cut(s)	1929, 2258
AccI	GTMKAC	1 cut(s)	2411
AccII	CGCG	1 cut(s)	252
AccIII	TCCGGA	1 cut(s)	368
AciI	CCGC	6 cut(s)	252, 2361, 2559, 2585, 2617, 2744
AclWI	GGATC	1 cut(s)	2449
AcoI	YGGCCR	1 cut(s)	31
AcsI	RAATTY	3 cut(s)	850, 1297, 1484
AdeI	CACNNNGTG	1 cut(s)	491
AfaI	GTAC	3 cut(s)	414, 732, 1622
AfeI	AGCGCT	2 cut(s)	935, 2806
AflII	CTTAAG	2 cut(s)	1172, 2762
AflIII	ACRYGT	1 cut(s)	733
AhlI	ACTAGT	1 cut(s)	2774
AjiI	CACGTC	1 cut(s)	2258
AjnI	CCWGG	6 cut(s)	658, 856, 1390, 1510, 1638, 1983
AjuI	GAANNNNNNNTTGG	4 cut(s)	970, 1002, 2312, 2344
Alw21I	GWGCWC	5 cut(s)	160, 408, 1875, 1982, 2489
Alw26I	GTCTC	1 cut(s)	1313
AlwI	GGATC	1 cut(s)	2449
AlwNI	CAGNNNCTG	3 cut(s)	518, 920, 2651
Ama87I	CYCGRG	4 cut(s)	717, 1413, 2674, 2800
Aor13HI	TCCGGA	1 cut(s)	368
Aor51HI	AGCGCT	2 cut(s)	935, 2806
AoxI	GGCC	9 cut(s)	23, 31, 134, 355, 859, 1147, 1388, 1442, 2542
ApoI	RAATTY	3 cut(s)	850, 1297, 1484
ArsI	GACNNNNNNTTYG	2 cut(s)	2135, 2167
Asp700I	GAANNNNTTC	1 cut(s)	1270
AspLEI	GCGC	4 cut(s)	936, 997, 1648, 2807
AspS9I	GGNCC	7 cut(s)	356, 860, 1898, 2249, 2334, 2542, 2663
AsuC2I	CCSGG	2 cut(s)	1414, 1415
AsuHPI	GGTGA	2 cut(s)	1406, 2453
AsuNHI	GCTAGC	2 cut(s)	1021, 2795
AvaI	CYCGRG	4 cut(s)	717, 1413, 2674, 2800
AvaII	GGWCC	4 cut(s)	1898, 2249, 2334, 2663
BaeGI	GKGCMC	1 cut(s)	1036
BalI	TGGCCA	1 cut(s)	33
BanI	GGYRCC	1 cut(s)	1829
BanII	GRGCYC	5 cut(s)	160, 408, 667, 1508, 2637
Bbv12I	GWGCWC	5 cut(s)	160, 408, 1875, 1982, 2489
BceAI	ACGGC	3 cut(s)	1549, 2006, 2061
BcgI	CGANNNNNNTGC	2 cut(s)	2810, 2844
BciT130I	CCWGG	6 cut(s)	660, 858, 1392, 1512, 1640, 1985
BciVI	GTATCC	1 cut(s)	1517
BclI	TGATCA	2 cut(s)	235, 1213
BcnI	CCSGG	2 cut(s)	1414, 1415
BcoDI	GTCTC	1 cut(s)	1313
BcuI	ACTAGT	1 cut(s)	2774
BfmI	CTRYAG	2 cut(s)	1160, 2596
BfoI	RGCGCY	2 cut(s)	937, 2808
BfrI	CTTAAG	2 cut(s)	1172, 2762
BfuI	GTATCC	1 cut(s)	1517
BglII	AGATCT	2 cut(s)	633, 2678
Bme1390I	CCNGG	8 cut(s)	660, 858, 1392, 1414, 1415, 1512, 1640, 1985
Bme18I	GGWCC	4 cut(s)	1898, 2249, 2334, 2663
BmeT110I	CYCGRG	4 cut(s)	717, 1413, 2674, 2800
BmgBI	CACGTC	1 cut(s)	2258
BmgT120I	GGNCC	7 cut(s)	356, 860, 1898, 2249, 2334, 2542, 2663
BmiI	GGNNCC	5 cut(s)	664, 862, 1273, 1831, 2543
BmrFI	CCNGG	8 cut(s)	660, 858, 1392, 1414, 1415, 1512, 1640, 1985
BmsI	GCATC	3 cut(s)	598, 1651, 2500
BmtI	GCTAGC	2 cut(s)	1025, 2799
BpmI	CTGGAG	4 cut(s)	341, 681, 1308, 1494
BpuEI	CTTGAG	2 cut(s)	1778, 1889
BpuMI	CCSGG	2 cut(s)	1414, 1415
BsaBI	GATNNNNATC	2 cut(s)	921, 1224
BsaI	GGTCTC	1 cut(s)	1313
BsaWI	WCCGGW	2 cut(s)	368, 1875
BsaXI	ACNNNNNCTCC	2 cut(s)	1842, 1872
Bse1I	ACTGG	3 cut(s)	34, 1273, 2703
Bse3DI	GCAATG	2 cut(s)	768, 1317
Bse8I	GATNNNNATC	2 cut(s)	921, 1224
BseAI	TCCGGA	1 cut(s)	368
BseBI	CCWGG	6 cut(s)	660, 858, 1392, 1512, 1640, 1985
BseGI	GGATG	5 cut(s)	545, 1346, 1424, 1884, 2369
BseJI	GATNNNNATC	2 cut(s)	921, 1224
BseMI	GCAATG	2 cut(s)	768, 1317
BseMII	CTCAG	4 cut(s)	1326, 1608, 2445, 2643
BseNI	ACTGG	3 cut(s)	34, 1273, 2703
BseRI	GAGGAG	2 cut(s)	2382, 2669
BseSI	GKGCMC	1 cut(s)	1036
BseYI	CCCAGC	1 cut(s)	101
Bsh1236I	CGCG	1 cut(s)	252
BshFI	GGCC	9 cut(s)	25, 33, 136, 357, 861, 1149, 1390, 1444, 2544
BshNI	GGYRCC	1 cut(s)	1829
BsiHKAI	GWGCWC	5 cut(s)	160, 408, 1875, 1982, 2489
BsiHKCI	CYCGRG	4 cut(s)	717, 1413, 2674, 2800
BsiSI	CCGG	3 cut(s)	369, 1414, 1876
BslFI	GGGAC	5 cut(s)	118, 268, 985, 1752, 2001
BsmAI	GTCTC	1 cut(s)	1313
BsmFI	GGGAC	5 cut(s)	118, 268, 985, 1752, 2001
BsmI	GAATGC	3 cut(s)	121, 2375, 2821
BsnI	GGCC	9 cut(s)	25, 33, 136, 357, 861, 1149, 1390, 1444, 2544
Bso31I	GGTCTC	1 cut(s)	1313
BsoBI	CYCGRG	4 cut(s)	717, 1413, 2674, 2800
Bsp1286I	GDGCHC	9 cut(s)	160, 408, 667, 1036, 1508, 1875, 1982, 2489, 2637
Bsp13I	TCCGGA	1 cut(s)	368
Bsp1407I	TGTACA	1 cut(s)	730
BspACI	CCGC	6 cut(s)	252, 2361, 2559, 2585, 2617, 2744
BspANI	GGCC	9 cut(s)	25, 33, 136, 357, 861, 1149, 1390, 1444, 2544
BspCNI	CTCAG	4 cut(s)	1325, 1609, 2444, 2644
BspEI	TCCGGA	1 cut(s)	368
BspFNI	CGCG	1 cut(s)	252
BspHI	TCATGA	2 cut(s)	1885, 1917
BspLI	GGNNCC	5 cut(s)	664, 862, 1273, 1831, 2543
BspOI	GCTAGC	2 cut(s)	1025, 2799
BspPI	GGATC	1 cut(s)	2449
BspQI	GCTCTTC	1 cut(s)	903
BspT107I	GGYRCC	1 cut(s)	1829
BspTI	CTTAAG	2 cut(s)	1172, 2762
BspTNI	GGTCTC	1 cut(s)	1313
BsrDI	GCAATG	2 cut(s)	768, 1317
BsrGI	TGTACA	1 cut(s)	730
BsrI	ACTGG	3 cut(s)	34, 1273, 2703
BssT1I	CCWWGG	4 cut(s)	612, 774, 1085, 1781
Bst2UI	CCWGG	6 cut(s)	660, 858, 1392, 1512, 1640, 1985
Bst4CI	ACNGT	7 cut(s)	310, 1529, 1975, 2071, 2338, 2441, 2480
Bst6I	CTCTTC	1 cut(s)	903
BstAFI	CTTAAG	2 cut(s)	1172, 2762
BstAPI	GCANNNNNTGC	1 cut(s)	1031
BstAUI	TGTACA	1 cut(s)	730
BstC8I	GCNNGC	5 cut(s)	510, 1023, 1442, 2569, 2797
BstDEI	CTNAG	5 cut(s)	630, 1312, 1617, 2431, 2652
BstDSI	CCRYGG	3 cut(s)	1577, 1894, 2637
BstENI	CCTNNNNNAGG	1 cut(s)	2831
BstF5I	GGATG	5 cut(s)	545, 1346, 1424, 1884, 2369
BstFNI	CGCG	1 cut(s)	252
BstH2I	RGCGCY	2 cut(s)	937, 2808
BstHHI	GCGC	4 cut(s)	936, 997, 1648, 2807
BstMAI	GTCTC	1 cut(s)	1313
BstMWI	GCNNNNNNNGC	8 cut(s)	39, 200, 209, 249, 1001, 1031, 1643, 2541
BstNI	CCWGG	6 cut(s)	660, 858, 1392, 1512, 1640, 1985
BstNSI	RCATGY	5 cut(s)	79, 512, 737, 2571, 2761
BstSCI	CCNGG	8 cut(s)	658, 856, 1390, 1412, 1413, 1510, 1638, 1983
BstSFI	CTRYAG	2 cut(s)	1160, 2596
BstSLI	GKGCMC	1 cut(s)	1036
BstUI	CGCG	1 cut(s)	252
BstX2I	RGATCY	3 cut(s)	633, 2454, 2678
BstYI	RGATCY	3 cut(s)	633, 2454, 2678
BsuI	GTATCC	1 cut(s)	1517
BsuRI	GGCC	9 cut(s)	25, 33, 136, 357, 861, 1149, 1390, 1444, 2544
BtgI	CCRYGG	3 cut(s)	1577, 1894, 2637
BtgZI	GCGATG	1 cut(s)	621
BtrI	CACGTC	1 cut(s)	2258
BtsCI	GGATG	5 cut(s)	545, 1346, 1424, 1884, 2369
BtsI	GCAGTG	6 cut(s)	53, 298, 1112, 1362, 1401, 1445
Cac8I	GCNNGC	5 cut(s)	510, 1023, 1442, 2569, 2797
CaiI	CAGNNNCTG	3 cut(s)	518, 920, 2651
CciI	TCATGA	2 cut(s)	1885, 1917
CfoI	GCGC	4 cut(s)	936, 997, 1648, 2807
Cfr13I	GGNCC	7 cut(s)	356, 860, 1898, 2249, 2334, 2542, 2663
Cfr9I	CCCGGG	1 cut(s)	1413
CseI	GACGC	1 cut(s)	2694
Csp6I	GTAC	3 cut(s)	413, 731, 1621
CspCI	CAANNNNNGTGG	2 cut(s)	1039, 1074
CviQI	GTAC	3 cut(s)	413, 731, 1621
DdeI	CTNAG	5 cut(s)	630, 1312, 1617, 2431, 2652
DraI	TTTAAA	1 cut(s)	2233
DraIII	CACNNNGTG	1 cut(s)	491
EaeI	YGGCCR	1 cut(s)	31
Eam1104I	CTCTTC	1 cut(s)	903
EarI	CTCTTC	1 cut(s)	903
Ecl136II	GAGCTC	2 cut(s)	158, 406
Eco130I	CCWWGG	4 cut(s)	612, 774, 1085, 1781
Eco147I	AGGCCT	1 cut(s)	25
Eco24I	GRGCYC	5 cut(s)	160, 408, 667, 1508, 2637
Eco31I	GGTCTC	1 cut(s)	1313
Eco32I	GATATC	2 cut(s)	679, 2115
Eco47I	GGWCC	4 cut(s)	1898, 2249, 2334, 2663
Eco47III	AGCGCT	2 cut(s)	935, 2806
Eco53kI	GAGCTC	2 cut(s)	158, 406
Eco88I	CYCGRG	4 cut(s)	717, 1413, 2674, 2800
EcoICRI	GAGCTC	2 cut(s)	158, 406
EcoNI	CCTNNNNNAGG	1 cut(s)	2831
EcoO109I	RGGNCCY	1 cut(s)	2542
EcoRII	CCWGG	6 cut(s)	658, 856, 1390, 1510, 1638, 1983
EcoRV	GATATC	2 cut(s)	679, 2115
EcoT14I	CCWWGG	4 cut(s)	612, 774, 1085, 1781
EcoT22I	ATGCAT	2 cut(s)	510, 1006
EcoT38I	GRGCYC	5 cut(s)	160, 408, 667, 1508, 2637
ErhI	CCWWGG	4 cut(s)	612, 774, 1085, 1781
FalI	AAGNNNNNCTT	5 cut(s)	635, 667, 1166, 1198, 2829
FaqI	GGGAC	5 cut(s)	118, 268, 985, 1752, 2001
FauI	CCCGC	1 cut(s)	245
FbaI	TGATCA	2 cut(s)	235, 1213
FblI	GTMKAC	1 cut(s)	2411
FokI	GGATG	5 cut(s)	552, 1333, 1431, 1891, 2376
FriOI	GRGCYC	5 cut(s)	160, 408, 667, 1508, 2637
FspI	TGCGCA	1 cut(s)	1647
GlaI	GCGC	4 cut(s)	935, 996, 1647, 2806
GsaI	CCCAGC	1 cut(s)	105
GsuI	CTGGAG	4 cut(s)	341, 681, 1308, 1494
HaeII	RGCGCY	2 cut(s)	937, 2808
HaeIII	GGCC	9 cut(s)	25, 33, 136, 357, 861, 1149, 1390, 1444, 2544
HapII	CCGG	3 cut(s)	369, 1414, 1876
HgaI	GACGC	1 cut(s)	2694
HhaI	GCGC	4 cut(s)	936, 997, 1648, 2807
Hin6I	GCGC	4 cut(s)	934, 995, 1646, 2805
HinP1I	GCGC	4 cut(s)	934, 995, 1646, 2805
HincII	GTYRAC	3 cut(s)	2404, 2412, 2700
HindII	GTYRAC	3 cut(s)	2404, 2412, 2700
HindIII	AAGCTT	2 cut(s)	979, 2533
HpaII	CCGG	3 cut(s)	369, 1414, 1876
HphI	GGTGA	2 cut(s)	1406, 2453
Hpy166II	GTNNAC	9 cut(s)	165, 703, 1555, 1901, 2352, 2404, 2412, 2663, 2700
Hpy188I	TCNGA	9 cut(s)	147, 235, 479, 1203, 1254, 1520, 1812, 2135, 2434
Hpy8I	GTNNAC	9 cut(s)	165, 703, 1555, 1901, 2352, 2404, 2412, 2663, 2700
Hpy99I	CGWCG	1 cut(s)	622
HpyAV	CCTTC	7 cut(s)	661, 840, 962, 1049, 1843, 2090, 2837
HpyCH4III	ACNGT	7 cut(s)	310, 1529, 1975, 2071, 2338, 2441, 2480
HpyCH4IV	ACGT	2 cut(s)	1583, 2257
HpyF10VI	GCNNNNNNNGC	8 cut(s)	39, 200, 209, 249, 1001, 1031, 1643, 2541
HpyF3I	CTNAG	5 cut(s)	630, 1312, 1617, 2431, 2652
HpySE526I	ACGT	2 cut(s)	1583, 2257
HspAI	GCGC	4 cut(s)	934, 995, 1646, 2805
Kpn2I	TCCGGA	1 cut(s)	368
Ksp22I	TGATCA	2 cut(s)	235, 1213
LguI	GCTCTTC	1 cut(s)	903
LmnI	GCTCC	8 cut(s)	155, 322, 411, 431, 662, 951, 1327, 1850
LweI	GCATC	3 cut(s)	598, 1651, 2500
MaeII	ACGT	2 cut(s)	1583, 2257
MaeIII	GTNAC	5 cut(s)	491, 1399, 1475, 1678, 1822
MboII	GAAGA	9 cut(s)	239, 277, 594, 890, 1039, 1828, 2414, 2584, 2849
MfeI	CAATTG	1 cut(s)	216
MflI	RGATCY	3 cut(s)	633, 2454, 2678
MhlI	GDGCHC	9 cut(s)	160, 408, 667, 1036, 1508, 1875, 1982, 2489, 2637
MlsI	TGGCCA	1 cut(s)	33
MluNI	TGGCCA	1 cut(s)	33
MlyI	GAGTC	4 cut(s)	740, 967, 1004, 2193
MmeI	TCCRAC	2 cut(s)	1651, 2337
Mox20I	TGGCCA	1 cut(s)	33
Mph1103I	ATGCAT	2 cut(s)	510, 1006
MroI	TCCGGA	1 cut(s)	368
MroXI	GAANNNNTTC	1 cut(s)	1270
MscI	TGGCCA	1 cut(s)	33
MseI	TTAA	9 cut(s)	399, 738, 755, 1121, 1173, 2024, 2205, 2232, 2763
MslI	CAYNNNNRTG	2 cut(s)	80, 92
Msp20I	TGGCCA	1 cut(s)	33
MspA1I	CMGCKG	3 cut(s)	1667, 2080, 2818
MspCI	CTTAAG	2 cut(s)	1172, 2762
MspI	CCGG	3 cut(s)	369, 1414, 1876
MspR9I	CCNGG	8 cut(s)	660, 858, 1392, 1414, 1415, 1512, 1640, 1985
MunI	CAATTG	1 cut(s)	216
Mva1269I	GAATGC	3 cut(s)	121, 2375, 2821
MvaI	CCWGG	6 cut(s)	660, 858, 1392, 1512, 1640, 1985
MvnI	CGCG	1 cut(s)	252
MwoI	GCNNNNNNNGC	8 cut(s)	39, 200, 209, 249, 1001, 1031, 1643, 2541
NciI	CCSGG	2 cut(s)	1414, 1415
NheI	GCTAGC	2 cut(s)	1021, 2795
NlaIV	GGNNCC	5 cut(s)	664, 862, 1273, 1831, 2543
NmeAIII	GCCGAG	1 cut(s)	162
NmuCI	GTSAC	3 cut(s)	491, 1399, 1822
NsbI	TGCGCA	1 cut(s)	1647
NsiI	ATGCAT	2 cut(s)	510, 1006
NspI	RCATGY	5 cut(s)	79, 512, 737, 2571, 2761
PaeI	GCATGC	2 cut(s)	512, 2571
PagI	TCATGA	2 cut(s)	1885, 1917
PasI	CCCWGGG	1 cut(s)	1984
PceI	AGGCCT	1 cut(s)	25
PciI	ACATGT	1 cut(s)	733
PciSI	GCTCTTC	1 cut(s)	903
PctI	GAATGC	3 cut(s)	121, 2375, 2821
PdmI	GAANNNNTTC	1 cut(s)	1270
PflMI	CCANNNNNTGG	2 cut(s)	1929, 2258
PfoI	TCCNGGA	1 cut(s)	658
PleI	GAGTC	4 cut(s)	740, 967, 1004, 2193
PpsI	GAGTC	4 cut(s)	740, 967, 1004, 2193
PscI	ACATGT	1 cut(s)	733
Psp124BI	GAGCTC	2 cut(s)	160, 408
Psp6I	CCWGG	6 cut(s)	658, 856, 1390, 1510, 1638, 1983
PspFI	CCCAGC	1 cut(s)	101
PspGI	CCWGG	6 cut(s)	658, 856, 1390, 1510, 1638, 1983
PspN4I	GGNNCC	5 cut(s)	664, 862, 1273, 1831, 2543
PspPI	GGNCC	7 cut(s)	356, 860, 1898, 2249, 2334, 2542, 2663
PsrI	GAACNNNNNNTAC	2 cut(s)	43, 75
PstNI	CAGNNNCTG	3 cut(s)	518, 920, 2651
PsuI	RGATCY	3 cut(s)	633, 2454, 2678
PvuII	CAGCTG	3 cut(s)	1667, 2080, 2818
RsaI	GTAC	3 cut(s)	414, 732, 1622
RsaNI	GTAC	3 cut(s)	413, 731, 1621
RseI	CAYNNNNRTG	2 cut(s)	80, 92
SacI	GAGCTC	2 cut(s)	160, 408
SalI	GTCGAC	1 cut(s)	2410
SapI	GCTCTTC	1 cut(s)	903
SaqAI	TTAA	9 cut(s)	399, 738, 755, 1121, 1173, 2024, 2205, 2232, 2763
Sau96I	GGNCC	7 cut(s)	356, 860, 1898, 2249, 2334, 2542, 2663
SchI	GAGTC	4 cut(s)	740, 967, 1004, 2193
ScrFI	CCNGG	8 cut(s)	660, 858, 1392, 1414, 1415, 1512, 1640, 1985
SduI	GDGCHC	9 cut(s)	160, 408, 667, 1036, 1508, 1875, 1982, 2489, 2637
SfaNI	GCATC	3 cut(s)	598, 1651, 2500
SfcI	CTRYAG	2 cut(s)	1160, 2596
SinI	GGWCC	4 cut(s)	1898, 2249, 2334, 2663
SmaI	CCCGGG	1 cut(s)	1415
SmiMI	CAYNNNNRTG	2 cut(s)	80, 92
SmlI	CTYRAG	4 cut(s)	1172, 1793, 1868, 2762
SmoI	CTYRAG	4 cut(s)	1172, 1793, 1868, 2762
SpeI	ACTAGT	1 cut(s)	2774
SphI	GCATGC	2 cut(s)	512, 2571
SseBI	AGGCCT	1 cut(s)	25
SsiI	CCGC	6 cut(s)	252, 2361, 2559, 2585, 2617, 2744
SspI	AATATT	2 cut(s)	151, 1258
SstI	GAGCTC	2 cut(s)	160, 408
StuI	AGGCCT	1 cut(s)	25
StyD4I	CCNGG	8 cut(s)	658, 856, 1390, 1412, 1413, 1510, 1638, 1983
StyI	CCWWGG	4 cut(s)	612, 774, 1085, 1781
TaaI	ACNGT	7 cut(s)	310, 1529, 1975, 2071, 2338, 2441, 2480
TaiI	ACGT	2 cut(s)	1586, 2260
TaqI	TCGA	4 cut(s)	1105, 1468, 2128, 2411
TatI	WGTACW	2 cut(s)	730, 1620
Tru1I	TTAA	9 cut(s)	399, 738, 755, 1121, 1173, 2024, 2205, 2232, 2763
Tru9I	TTAA	9 cut(s)	399, 738, 755, 1121, 1173, 2024, 2205, 2232, 2763
TseFI	GTSAC	3 cut(s)	491, 1399, 1822
Tsp45I	GTSAC	3 cut(s)	491, 1399, 1822
TspDTI	ATGAA	8 cut(s)	32, 887, 981, 1068, 1193, 1895, 2195, 2296
TspGWI	ACGGA	5 cut(s)	833, 1121, 1594, 1883, 2104
TspMI	CCCGGG	1 cut(s)	1413
Van91I	CCANNNNNTGG	2 cut(s)	1929, 2258
Vha464I	CTTAAG	2 cut(s)	1172, 2762
VpaK11BI	GGWCC	4 cut(s)	1898, 2249, 2334, 2663
XagI	CCTNNNNNAGG	1 cut(s)	2831
XapI	RAATTY	3 cut(s)	850, 1297, 1484
XbaI	TCTAGA	3 cut(s)	944, 2195, 2681
XceI	RCATGY	5 cut(s)	79, 512, 737, 2571, 2761
XcmI	CCANNNNNNNNNTGG	1 cut(s)	94
XmaI	CCCGGG	1 cut(s)	1413
XmiI	GTMKAC	1 cut(s)	2411
XmnI	GAANNNNTTC	1 cut(s)	1270
Zsp2I	ATGCAT	2 cut(s)	510, 1006

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.