Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0001061

Physical Location & Seq

Genomic Coordinates Reverse (-)

7990522 .. 7993582

Gene Structure 3061 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1108100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2580 bp

ATGGAGCTGATTGAAAATGACGTGGTTGCTGCAGTCGGCCCACAATCCTCTGGAATAGCTCATGTCATATCCCATGTTGTTAATGAGCTCCATGTACCACTTCTATCATTTGGAGCAACAGACCCCTCCCTTGCTGCTCTCCAATACCCATATTTTGTCCGAACCACACAGAGTGACTATTTCCAAATGTATGCAGTTGCTGATTTAGTTGGGTATTTTGGATGGAGAGAAGTAATAGCAATCTTTGTAGATGATGATTATGGCAGAAATGGAATTTCTATACTAGGCGATACCTTGGCGGTGAAGCGTTCTAAGATCTCATACAAGGCTGCCTTCTCTCCTGGAGCCCCCAAAAGCAATATCAATGAATTGTTGGTGGGAGTGAACCTCATGGAATCTCGGGTTTATATTGTACATGTTAATCCCGACTCCGGTTTAACTATTTTTTCCATTGCCAAGGCACTTGGAATGATGACCAGTGGCTATGTCTGGATTGCGACGGATTGGCTTCCTTCTCATTTAGATTCACTAAATCCCCCTGGCCCTGACATAATGAATCTCTTACAAGGGGTTGTTGCTCTTCGTCATCATACCCCAGATACTGATCTGAAAAAGCGCTTTATGTCTAGATGGAGCAAATTGCAACATGACGGGAGTCCAAGCTTCAATTCTTATGCGCTCTATGCATACGACTCTATTTGGCTTGCAGCCCGTGCTCTTGATGTTTTCTTCAACGAAGGTGGGAATGTATCTTTCTCTAATGACCCAAGGTTGAAAGACACAAATCGAAGCACACTGCATTTAACATCACTCCGTATTTTTGACGGAGGCCAAAAATATCTACAGACGATTCTTAAGATGAACTTCACAGGTATAAGTGGTCAGATTGAGTTTGATCAAGATAAATATCTAGTTCGTCCAACATATGACATTCTGAATATTGGAGGAACTGGTTCCCGTAGAGTTGGCTATTGGTCAAATTCTAGTGGTCTCTCAGTCATTGCTCCTGAGAGAATATATAAGAAGCCATCCAATAGGAATACCACTGCTCAACTTTATAGCGTTATATGGCCTGGTGAAGTCACTGCTATACCTCGGGGATGGGTATTTCCCAACAATGGCAGGCCACTGCGAATTGGAGTGCCTTATCGAGTAAGTTACAAAGATTTTGTGGTTAAAGACAAGAGCCCTCCAGGGGTCACAGGATACTGTATTGATGTCTTTGAAGCTGCTGTAAACTTATTGCCGTATGCTGTGCCACGGACATATATGTTGTATGGAGATGGTAAGAGGAATCCTGAGTACAGCAGTCTTATAGCCCAGGTTGCGCAAAATAAATTTGATGCAGCTGTTGGAGATGTTACAATTACTACTAATAGGACAAGAATAGTTGATTTTACACAGCCTTACATGGAATCGGGACTAGTTGTAGTTGTTCCTGTCAAAAAGGCAAAATCAAACCCTTGGGTTTTCCTCAAGCCATTTACTTATCAGATGTGGTTGGTCACCGGTGCCTTCTTCCTTTTAGTGGGAGCTGTTGTATGGATTCTTGAGCACCGGATCAATCATGAGTTCCGTGGTTCACCGAGCCAACAGCTCATAACCATTTTTTGGTCAGTATGTCTTGCATCCCTATGGCTATTGATAAGTGCCTATACGAGAGAGAACACTGTGAGCACCCTGGGACGGCTGGTGCTGATTATATGGTTATTTGTAGTGTTAATTATCAATTCCAGTTACACGGCTAGTTTGACTTCAATTCTCACGGTGCAACGGCTGACATCACGGATTGAAGGGATTGACAGCTTGATATCAAGTAATGATCGAATCGGAGTTCAAGACGGGTCATTCGTTTGGAGGTATTTGGTTGATGAGATGAACATAGCGGAATCTAGACTGGTTAAGTTGGAAGACATGGAAGCCTATATTAAAGCCCTTACAGATGGACCAAGACGTGGTGGGGTAGCTGCCATCGTTGATGAGCTTCCTTACATTGAGTTGTTTATGTCCAGCACCAAATGCGCATTCAGGACAGTAGGACTGGAGTTTAGCAACAGCGGATGGGGATTTGCCTTCCAAAAGGATTCTCCTTTTGCTGTTGACTTGTCGACTGCAATTCTTCAACTCTCAGAGAACGGTGATCTACAAAAGATCCATAATAAGTGGCTTACGCATAACGAGTGCCCTACTCAACTCAATGACGTTGATGCAGACCTGCAGCTATCTCTAACAAGCTTTTGGGGCCTGTTTCTTATCTGCGGTATTGCATGCTTCCTTGCTCTTGCGGTGTTCTTCTGTAGGATCCTTTGTCAATACCGCAGATTTACCCCCGAGCCTGTGGAAGCGGACGTTGAGGAGATTGGACCTACCAACACGAGATCTCGACGCTCACTCTGGTCAACTATTTTCAAGGATCTTATGGTCCTTGTAGATAAGAAAGAAGCAGAGATAAAGCACATGCTTAAGCGAAAGACTAGTGATAGTAAGCACGAAGCTAGCCCGAGCGCTGATGGGCAGCTGCATTCCCTTCGTGAGGAAGATTTGAGCGCTGATGGGCGGCTGCATTCCCTTCGTACGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0004888 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0007154 GO:0007165 GO:0008066 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009507 GO:0009536 GO:0009605 GO:0009611 GO:0009612 GO:0009628 GO:0009719 GO:0009987 GO:0010033 GO:0010034 GO:0010243 GO:0015075 GO:0015085 GO:0015267 GO:0015276 GO:0015318 GO:0016020 GO:0019722 GO:0019932 GO:0022803 GO:0022834 GO:0022836 GO:0022838 GO:0022839 GO:0022857 GO:0022890 GO:0023052 GO:0030001 GO:0033554 GO:0034220 GO:0035556 GO:0038023 GO:0042221 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046677 GO:0046873 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055085 GO:0060089 GO:0065007 GO:0070417 GO:0070588 GO:0070838 GO:0070887 GO:0071214 GO:0071229 GO:0071230 GO:0071236 GO:0071260 GO:0071310 GO:0071311 GO:0071417 GO:0071495 GO:0071496 GO:0071944 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:0104004 GO:1901698 GO:1901699 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

860

Amino Acids

96.2

Weight (kDa)

6.8

Isoelectric Point (pI)

41.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ANF_receptor	PF01094	2 - 316	5.8e-73	Receptor family ligand binding region
Peripla_BP_6	PF13458	2 - 240	6.7e-11	Periplasmic binding protein
Lig_chan-Glu_bd	PF10613	393 - 481	7.5e-12	Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3	PF00497	434 - 724	1.8e-13	Bacterial extracellular solute-binding proteins, family 3
Lig_chan	PF00060	497 - 757	1e-32	Ligand-gated ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0001061)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G05200 AT1G05200 AT1G05200 AT1G05200 AT1G05200 AT1G05200 AT1G05200 AT2G32390 AT2G32390 AT2G32390 AT2G32390 AT2G32390 AT2G32390 AT2G32390
fragaria_vesca	FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291
malus_domestica	MD09G1069900.v1.1 MD09G1070100.v1.1 MD09G1107700.v1.1 MD09G1108100.v1.1 MD17G1096100.v1.1
prunus_persica	Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1
pyrus_communis	pycom09g03000 pycom09g03030 pycom09g03050 pycom09g03070 pycom15g20430 pycom17g09220
rosa_chinensis	RchiOBHm_Chr1g0324131 RchiOBHm_Chr2g0159571 RchiOBHm_Chr2g0159581
rosa_laevigata	RLG00000021167
rosa_multiflora	Rmu_co8397097.1_g000001 Rmu_sc0004966.1_g000017 Rmu_sc0020031.1_g000001
rosa_roxburghii	Rroxscaffold_2G00090390
rosa_rugosa	Rorug02G0476900 Rorug02G0477000 Rorug02G0477100 Rorug02G0477200
rosa_samantha	Rh2AG544200 Rh2BG557000 Rh2CG527200 Rh2DG566000
rosa_wichuraiana	Rw2G044990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	2 cut(s)	1329, 2023
Acc36I	ACCTGC	1 cut(s)	2223
AccB1I	GGYRCC	1 cut(s)	1511
AccB7I	CCANNNNNTGG	2 cut(s)	1611, 1955
AccI	GTMKAC	1 cut(s)	2108
AciI	CCGC	8 cut(s)	299, 1886, 2058, 2259, 2285, 2317, 2345, 2557
AclWI	GGATC	5 cut(s)	1568, 2146, 2296, 2309, 2421
AcsI	RAATTY	3 cut(s)	273, 979, 1337
AdeI	CACNNNGTG	1 cut(s)	173
AfaI	GTAC	4 cut(s)	96, 414, 1304, 2575
AfeI	AGCGCT	3 cut(s)	617, 2506, 2548
AfiI	CCNNNNNNNGG	8 cut(s)	431, 1118, 1195, 1528, 1611, 1686, 1955, 2533
AflII	CTTAAG	2 cut(s)	854, 2462
AflIII	ACRYGT	1 cut(s)	415
AgeI	ACCGGT	1 cut(s)	1508
AhlI	ACTAGT	2 cut(s)	1423, 2474
AjiI	CACGTC	2 cut(s)	22, 1955
AjnI	CCWGG	6 cut(s)	340, 538, 1072, 1192, 1320, 1680
AjuI	GAANNNNNNNTTGG	4 cut(s)	652, 684, 2009, 2041
Alw21I	GWGCWC	4 cut(s)	90, 718, 1557, 1679
Alw26I	GTCTC	1 cut(s)	995
AlwI	GGATC	5 cut(s)	1568, 2146, 2296, 2309, 2421
AlwNI	CAGNNNCTG	2 cut(s)	200, 602
Ama87I	CYCGRG	4 cut(s)	399, 1095, 2330, 2500
Aor51HI	AGCGCT	3 cut(s)	617, 2506, 2548
AoxI	GGCC	6 cut(s)	37, 541, 829, 1070, 1124, 2242
ApoI	RAATTY	3 cut(s)	273, 979, 1337
ArsI	GACNNNNNNTTYG	2 cut(s)	1832, 1864
AsiGI	ACCGGT	1 cut(s)	1508
Asp700I	GAANNNNTTC	1 cut(s)	952
AspLEI	GCGC	6 cut(s)	618, 679, 1330, 2024, 2507, 2549
AspS9I	GGNCC	6 cut(s)	38, 542, 1946, 2242, 2363, 2422
AsuHPI	GGTGA	5 cut(s)	313, 1088, 1498, 1575, 2150
AsuNHI	GCTAGC	1 cut(s)	2495
AvaI	CYCGRG	4 cut(s)	399, 1095, 2330, 2500
AvaII	GGWCC	3 cut(s)	1946, 2363, 2422
BaeGI	GKGCMC	1 cut(s)	2186
BamHI	GGATCC	1 cut(s)	2301
BanI	GGYRCC	1 cut(s)	1511
BanII	GRGCYC	3 cut(s)	90, 349, 1190
BauI	CACGAG	1 cut(s)	2374
BbsI	GAAGAC	1 cut(s)	1917
Bbv12I	GWGCWC	4 cut(s)	90, 718, 1557, 1679
BceAI	ACGGC	4 cut(s)	1231, 1703, 1758, 1790
BcgI	CGANNNNNNTGC	3 cut(s)	2510, 2544, 2552
BciT130I	CCWGG	6 cut(s)	342, 540, 1074, 1194, 1322, 1682
BciVI	GTATCC	1 cut(s)	1199
BclI	TGATCA	1 cut(s)	895
BcoDI	GTCTC	1 cut(s)	995
BcuI	ACTAGT	2 cut(s)	1423, 2474
BfaI	CTAG	9 cut(s)	284, 627, 911, 984, 1424, 1746, 1893, 2475, 2496
BfmI	CTRYAG	4 cut(s)	30, 842, 2216, 2296
BfoI	RGCGCY	3 cut(s)	619, 2508, 2550
BfrI	CTTAAG	2 cut(s)	854, 2462
BfuAI	ACCTGC	1 cut(s)	2223
BfuI	GTATCC	1 cut(s)	1199
BglII	AGATCT	2 cut(s)	315, 2378
Bme1390I	CCNGG	6 cut(s)	342, 540, 1074, 1194, 1322, 1682
Bme18I	GGWCC	3 cut(s)	1946, 2363, 2422
BmeT110I	CYCGRG	4 cut(s)	399, 1095, 2330, 2500
BmgBI	CACGTC	2 cut(s)	22, 1955
BmgT120I	GGNCC	6 cut(s)	38, 542, 1946, 2242, 2363, 2422
BmiI	GGNNCC	5 cut(s)	346, 955, 1513, 2243, 2303
BmrFI	CCNGG	6 cut(s)	342, 540, 1074, 1194, 1322, 1682
BmsI	GCATC	3 cut(s)	1333, 1637, 2197
BmtI	GCTAGC	1 cut(s)	2499
BoxI	GACNNNNGTC	1 cut(s)	654
BpiI	GAAGAC	1 cut(s)	1917
BplI	GAGNNNNNCTC	2 cut(s)	372, 404
BpmI	CTGGAG	3 cut(s)	363, 1176, 2063
BpuEI	CTTGAG	2 cut(s)	1460, 1571
BsaAI	YACGTR	1 cut(s)	2577
BsaBI	GATNNNNATC	2 cut(s)	603, 906
BsaI	GGTCTC	1 cut(s)	995
BsaWI	WCCGGW	3 cut(s)	431, 1508, 1557
BsaXI	ACNNNNNCTCC	2 cut(s)	1524, 1554
Bsc4I	CCNNNNNNNGG	8 cut(s)	431, 1118, 1195, 1528, 1611, 1686, 1955, 2533
Bse118I	RCCGGY	1 cut(s)	1508
Bse1I	ACTGG	5 cut(s)	477, 955, 1734, 1902, 2046
Bse3DI	GCAATG	2 cut(s)	450, 999
Bse8I	GATNNNNATC	2 cut(s)	603, 906
BseBI	CCWGG	6 cut(s)	342, 540, 1074, 1194, 1322, 1682
BseGI	GGATG	5 cut(s)	227, 1028, 1106, 1628, 2066
BseJI	GATNNNNATC	2 cut(s)	603, 906
BseLI	CCNNNNNNNGG	8 cut(s)	431, 1118, 1195, 1528, 1611, 1686, 1955, 2533
BseMI	GCAATG	2 cut(s)	450, 999
BseMII	CTCAG	4 cut(s)	999, 1008, 1290, 2142
BseNI	ACTGG	5 cut(s)	477, 955, 1734, 1902, 2046
BseRI	GAGGAG	1 cut(s)	2369
BseSI	GKGCMC	1 cut(s)	2186
BshFI	GGCC	6 cut(s)	39, 543, 831, 1072, 1126, 2244
BshNI	GGYRCC	1 cut(s)	1511
BshTI	ACCGGT	1 cut(s)	1508
BsiHKAI	GWGCWC	4 cut(s)	90, 718, 1557, 1679
BsiHKCI	CYCGRG	4 cut(s)	399, 1095, 2330, 2500
BsiSI	CCGG	3 cut(s)	432, 1509, 1558
BsiWI	CGTACG	1 cut(s)	2573
BslFI	GGGAC	2 cut(s)	1434, 1698
BslI	CCNNNNNNNGG	8 cut(s)	431, 1118, 1195, 1528, 1611, 1686, 1955, 2533
BsmAI	GTCTC	1 cut(s)	995
BsmFI	GGGAC	2 cut(s)	1434, 1698
BsmI	GAATGC	3 cut(s)	2024, 2521, 2563
BsnI	GGCC	6 cut(s)	39, 543, 831, 1072, 1126, 2244
Bso31I	GGTCTC	1 cut(s)	995
BsoBI	CYCGRG	4 cut(s)	399, 1095, 2330, 2500
Bsp1286I	GDGCHC	7 cut(s)	90, 349, 718, 1190, 1557, 1679, 2186
Bsp1407I	TGTACA	1 cut(s)	412
BspACI	CCGC	8 cut(s)	299, 1886, 2058, 2259, 2285, 2317, 2345, 2557
BspANI	GGCC	6 cut(s)	39, 543, 831, 1072, 1126, 2244
BspCNI	CTCAG	4 cut(s)	1000, 1007, 1291, 2141
BspHI	TCATGA	1 cut(s)	1567
BspLI	GGNNCC	5 cut(s)	346, 955, 1513, 2243, 2303
BspMAI	CTGCAG	2 cut(s)	34, 2220
BspMI	ACCTGC	1 cut(s)	2223
BspOI	GCTAGC	1 cut(s)	2499
BspPI	GGATC	5 cut(s)	1568, 2146, 2296, 2309, 2421
BspQI	GCTCTTC	1 cut(s)	585
BspT107I	GGYRCC	1 cut(s)	1511
BspTI	CTTAAG	2 cut(s)	854, 2462
BspTNI	GGTCTC	1 cut(s)	995
BsrDI	GCAATG	2 cut(s)	450, 999
BsrFI	RCCGGY	1 cut(s)	1508
BsrGI	TGTACA	1 cut(s)	412
BsrI	ACTGG	5 cut(s)	477, 955, 1734, 1902, 2046
BssAI	RCCGGY	1 cut(s)	1508
BssSI	CACGAG	1 cut(s)	2374
BssT1I	CCWWGG	4 cut(s)	294, 456, 767, 1463
Bst2BI	CACGAG	1 cut(s)	2374
Bst2UI	CCWGG	6 cut(s)	342, 540, 1074, 1194, 1322, 1682
Bst4CI	ACNGT	5 cut(s)	1211, 1672, 1768, 2035, 2138
Bst6I	CTCTTC	1 cut(s)	585
BstAFI	CTTAAG	2 cut(s)	854, 2462
BstAPI	GCANNNNNTGC	2 cut(s)	713, 2019
BstAUI	TGTACA	1 cut(s)	412
BstBAI	YACGTR	1 cut(s)	2577
BstC8I	GCNNGC	4 cut(s)	705, 1124, 2269, 2497
BstDEI	CTNAG	5 cut(s)	312, 994, 1008, 1299, 2128
BstDSI	CCRYGG	2 cut(s)	1259, 1576
BstEII	GGTNACC	1 cut(s)	1504
BstENI	CCTNNNNNAGG	1 cut(s)	2531
BstF5I	GGATG	5 cut(s)	227, 1028, 1106, 1628, 2066
BstH2I	RGCGCY	3 cut(s)	619, 2508, 2550
BstHHI	GCGC	6 cut(s)	618, 679, 1330, 2024, 2507, 2549
BstMAI	GTCTC	1 cut(s)	995
BstMWI	GCNNNNNNNGC	5 cut(s)	683, 713, 1325, 2019, 2241
BstNI	CCWGG	6 cut(s)	342, 540, 1074, 1194, 1322, 1682
BstNSI	RCATGY	3 cut(s)	419, 2271, 2461
BstPAI	GACNNNNGTC	1 cut(s)	654
BstPI	GGTNACC	1 cut(s)	1504
BstSCI	CCNGG	6 cut(s)	340, 538, 1072, 1192, 1320, 1680
BstSFI	CTRYAG	4 cut(s)	30, 842, 2216, 2296
BstSLI	GKGCMC	1 cut(s)	2186
BstV2I	GAAGAC	1 cut(s)	1917
BstX2I	RGATCY	5 cut(s)	315, 2151, 2301, 2378, 2413
BstYI	RGATCY	5 cut(s)	315, 2151, 2301, 2378, 2413
BsuI	GTATCC	1 cut(s)	1199
BsuRI	GGCC	6 cut(s)	39, 543, 831, 1072, 1126, 2244
BtgI	CCRYGG	2 cut(s)	1259, 1576
BtrI	CACGTC	2 cut(s)	22, 1955
BtsCI	GGATG	5 cut(s)	227, 1028, 1106, 1628, 2066
BtsI	GCAGTG	4 cut(s)	794, 1044, 1083, 1127
BtsIMutI	CAGTG	6 cut(s)	484, 794, 1044, 1083, 1127, 1668
BveI	ACCTGC	1 cut(s)	2223
Cac8I	GCNNGC	4 cut(s)	705, 1124, 2269, 2497
CaiI	CAGNNNCTG	2 cut(s)	200, 602
CciI	TCATGA	1 cut(s)	1567
CfoI	GCGC	6 cut(s)	618, 679, 1330, 2024, 2507, 2549
Cfr10I	RCCGGY	1 cut(s)	1508
Cfr13I	GGNCC	6 cut(s)	38, 542, 1946, 2242, 2363, 2422
CseI	GACGC	1 cut(s)	2394
Csp6I	GTAC	4 cut(s)	95, 413, 1303, 2574
CspAI	ACCGGT	1 cut(s)	1508
CspCI	CAANNNNNGTGG	2 cut(s)	721, 756
CviQI	GTAC	4 cut(s)	95, 413, 1303, 2574
DdeI	CTNAG	5 cut(s)	312, 994, 1008, 1299, 2128
DraIII	CACNNNGTG	1 cut(s)	173
Eam1104I	CTCTTC	1 cut(s)	585
EarI	CTCTTC	1 cut(s)	585
Ecl136II	GAGCTC	1 cut(s)	88
Eco130I	CCWWGG	4 cut(s)	294, 456, 767, 1463
Eco24I	GRGCYC	3 cut(s)	90, 349, 1190
Eco31I	GGTCTC	1 cut(s)	995
Eco32I	GATATC	1 cut(s)	1812
Eco47I	GGWCC	3 cut(s)	1946, 2363, 2422
Eco47III	AGCGCT	3 cut(s)	617, 2506, 2548
Eco53kI	GAGCTC	1 cut(s)	88
Eco88I	CYCGRG	4 cut(s)	399, 1095, 2330, 2500
Eco91I	GGTNACC	1 cut(s)	1504
EcoICRI	GAGCTC	1 cut(s)	88
EcoNI	CCTNNNNNAGG	1 cut(s)	2531
EcoO109I	RGGNCCY	1 cut(s)	2242
EcoO65I	GGTNACC	1 cut(s)	1504
EcoRII	CCWGG	6 cut(s)	340, 538, 1072, 1192, 1320, 1680
EcoRV	GATATC	1 cut(s)	1812
EcoT14I	CCWWGG	4 cut(s)	294, 456, 767, 1463
EcoT22I	ATGCAT	1 cut(s)	688
EcoT38I	GRGCYC	3 cut(s)	90, 349, 1190
ErhI	CCWWGG	4 cut(s)	294, 456, 767, 1463
FalI	AAGNNNNNCTT	4 cut(s)	317, 349, 848, 880
FaqI	GGGAC	2 cut(s)	1434, 1698
FauNDI	CATATG	1 cut(s)	925
FbaI	TGATCA	1 cut(s)	895
FblI	GTMKAC	1 cut(s)	2108
FokI	GGATG	5 cut(s)	234, 1015, 1113, 1615, 2073
FriOI	GRGCYC	3 cut(s)	90, 349, 1190
FspAI	RTGCGCAY	1 cut(s)	2023
FspBI	CTAG	9 cut(s)	284, 627, 911, 984, 1424, 1746, 1893, 2475, 2496
FspI	TGCGCA	2 cut(s)	1329, 2023
GlaI	GCGC	6 cut(s)	617, 678, 1329, 2023, 2506, 2548
GsuI	CTGGAG	3 cut(s)	363, 1176, 2063
HaeII	RGCGCY	3 cut(s)	619, 2508, 2550
HaeIII	GGCC	6 cut(s)	39, 543, 831, 1072, 1126, 2244
HapII	CCGG	3 cut(s)	432, 1509, 1558
HgaI	GACGC	1 cut(s)	2394
HhaI	GCGC	6 cut(s)	618, 679, 1330, 2024, 2507, 2549
Hin6I	GCGC	6 cut(s)	616, 677, 1328, 2022, 2505, 2547
HinP1I	GCGC	6 cut(s)	616, 677, 1328, 2022, 2505, 2547
HincII	GTYRAC	3 cut(s)	2101, 2109, 2400
HindII	GTYRAC	3 cut(s)	2101, 2109, 2400
HindIII	AAGCTT	2 cut(s)	661, 2233
HpaII	CCGG	3 cut(s)	432, 1509, 1558
HphI	GGTGA	5 cut(s)	313, 1088, 1498, 1575, 2150
Hpy166II	GTNNAC	6 cut(s)	385, 1237, 1583, 2101, 2109, 2400
Hpy188I	TCNGA	7 cut(s)	161, 609, 885, 936, 1494, 1832, 2131
Hpy8I	GTNNAC	6 cut(s)	385, 1237, 1583, 2101, 2109, 2400
Hpy99I	CGWCG	2 cut(s)	502, 2388
HpyAV	CCTTC	8 cut(s)	343, 522, 731, 1525, 1787, 2083, 2537, 2579
HpyCH4III	ACNGT	5 cut(s)	1211, 1672, 1768, 2035, 2138
HpyCH4IV	ACGT	5 cut(s)	21, 1954, 2202, 2349, 2576
HpyF10VI	GCNNNNNNNGC	5 cut(s)	683, 713, 1325, 2019, 2241
HpyF3I	CTNAG	5 cut(s)	312, 994, 1008, 1299, 2128
HpySE526I	ACGT	5 cut(s)	21, 1954, 2202, 2349, 2576
HspAI	GCGC	6 cut(s)	616, 677, 1328, 2022, 2505, 2547
Ksp22I	TGATCA	1 cut(s)	895
LguI	GCTCTTC	1 cut(s)	585
LmnI	GCTCC	7 cut(s)	4, 93, 113, 344, 633, 1009, 1532
LweI	GCATC	3 cut(s)	1333, 1637, 2197
MaeI	CTAG	9 cut(s)	284, 627, 911, 984, 1424, 1746, 1893, 2475, 2496
MaeII	ACGT	5 cut(s)	21, 1954, 2202, 2349, 2576
MaeIII	GTNAC	7 cut(s)	173, 1081, 1157, 1198, 1360, 1504, 1736
MboII	GAAGA	7 cut(s)	572, 721, 1510, 1922, 2111, 2284, 2549
MflI	RGATCY	5 cut(s)	315, 2151, 2301, 2378, 2413
MhlI	GDGCHC	7 cut(s)	90, 349, 718, 1190, 1557, 1679, 2186
MlyI	GAGTC	3 cut(s)	422, 664, 686
MmeI	TCCRAC	3 cut(s)	944, 1333, 1887
Mph1103I	ATGCAT	1 cut(s)	688
MroXI	GAANNNNTTC	1 cut(s)	952
MslI	CAYNNNNRTG	1 cut(s)	1633
MspA1I	CMGCKG	3 cut(s)	1349, 2058, 2518
MspCI	CTTAAG	2 cut(s)	854, 2462
MspI	CCGG	3 cut(s)	432, 1509, 1558
MspR9I	CCNGG	6 cut(s)	342, 540, 1074, 1194, 1322, 1682
Mva1269I	GAATGC	3 cut(s)	2024, 2521, 2563
MvaI	CCWGG	6 cut(s)	342, 540, 1074, 1194, 1322, 1682
MwoI	GCNNNNNNNGC	5 cut(s)	683, 713, 1325, 2019, 2241
NdeI	CATATG	1 cut(s)	925
NheI	GCTAGC	1 cut(s)	2495
NlaIV	GGNNCC	5 cut(s)	346, 955, 1513, 2243, 2303
NmuCI	GTSAC	4 cut(s)	173, 1081, 1198, 1504
NsbI	TGCGCA	2 cut(s)	1329, 2023
NsiI	ATGCAT	1 cut(s)	688
NspI	RCATGY	3 cut(s)	419, 2271, 2461
PaeI	GCATGC	1 cut(s)	2271
PagI	TCATGA	1 cut(s)	1567
PasI	CCCWGGG	1 cut(s)	1681
PciI	ACATGT	1 cut(s)	415
PciSI	GCTCTTC	1 cut(s)	585
PcsI	WCGNNNNNNNCGW	1 cut(s)	1848
PctI	GAATGC	3 cut(s)	2024, 2521, 2563
PdmI	GAANNNNTTC	1 cut(s)	952
Pfl23II	CGTACG	1 cut(s)	2573
PflMI	CCANNNNNTGG	2 cut(s)	1611, 1955
PfoI	TCCNGGA	1 cut(s)	340
PinAI	ACCGGT	1 cut(s)	1508
PleI	GAGTC	3 cut(s)	422, 663, 686
PpsI	GAGTC	3 cut(s)	422, 663, 686
Ppu21I	YACGTR	1 cut(s)	2577
PscI	ACATGT	1 cut(s)	415
PshAI	GACNNNNGTC	1 cut(s)	654
Psp124BI	GAGCTC	1 cut(s)	90
Psp6I	CCWGG	6 cut(s)	340, 538, 1072, 1192, 1320, 1680
PspEI	GGTNACC	1 cut(s)	1504
PspGI	CCWGG	6 cut(s)	340, 538, 1072, 1192, 1320, 1680
PspLI	CGTACG	1 cut(s)	2573
PspN4I	GGNNCC	5 cut(s)	346, 955, 1513, 2243, 2303
PspPI	GGNCC	6 cut(s)	38, 542, 1946, 2242, 2363, 2422
PstI	CTGCAG	2 cut(s)	34, 2220
PstNI	CAGNNNCTG	2 cut(s)	200, 602
PsuI	RGATCY	5 cut(s)	315, 2151, 2301, 2378, 2413
PvuII	CAGCTG	2 cut(s)	1349, 2518
RsaI	GTAC	4 cut(s)	96, 414, 1304, 2575
RsaNI	GTAC	4 cut(s)	95, 413, 1303, 2574
RseI	CAYNNNNRTG	1 cut(s)	1633
SacI	GAGCTC	1 cut(s)	90
SalI	GTCGAC	1 cut(s)	2107
SapI	GCTCTTC	1 cut(s)	585
Sau96I	GGNCC	6 cut(s)	38, 542, 1946, 2242, 2363, 2422
SchI	GAGTC	3 cut(s)	422, 664, 686
ScrFI	CCNGG	6 cut(s)	342, 540, 1074, 1194, 1322, 1682
SduI	GDGCHC	7 cut(s)	90, 349, 718, 1190, 1557, 1679, 2186
SfaNI	GCATC	3 cut(s)	1333, 1637, 2197
SfcI	CTRYAG	4 cut(s)	30, 842, 2216, 2296
SgrAI	CRCCGGYG	1 cut(s)	1508
SinI	GGWCC	3 cut(s)	1946, 2363, 2422
SmiMI	CAYNNNNRTG	1 cut(s)	1633
SmlI	CTYRAG	4 cut(s)	854, 1475, 1550, 2462
SmoI	CTYRAG	4 cut(s)	854, 1475, 1550, 2462
SpeI	ACTAGT	2 cut(s)	1423, 2474
SphI	GCATGC	1 cut(s)	2271
SsiI	CCGC	8 cut(s)	299, 1886, 2058, 2259, 2285, 2317, 2345, 2557
SspI	AATATT	1 cut(s)	940
SspMI	CTAG	9 cut(s)	284, 627, 911, 984, 1424, 1746, 1893, 2475, 2496
SstI	GAGCTC	1 cut(s)	90
StyD4I	CCNGG	6 cut(s)	340, 538, 1072, 1192, 1320, 1680
StyI	CCWWGG	4 cut(s)	294, 456, 767, 1463
TaaI	ACNGT	5 cut(s)	1211, 1672, 1768, 2035, 2138
TaiI	ACGT	5 cut(s)	24, 1957, 2205, 2352, 2579
TaqI	TCGA	5 cut(s)	787, 1150, 1825, 2108, 2383
TatI	WGTACW	2 cut(s)	412, 1302
TauI	GCSGC	1 cut(s)	2560
TscAI	CASTG	6 cut(s)	484, 801, 1051, 1090, 1134, 1675
TseFI	GTSAC	4 cut(s)	173, 1081, 1198, 1504
Tsp45I	GTSAC	4 cut(s)	173, 1081, 1198, 1504
TspDTI	ATGAA	4 cut(s)	381, 569, 875, 1892
TspGWI	ACGGA	6 cut(s)	515, 803, 840, 1276, 1565, 1801
TspRI	CASTG	6 cut(s)	484, 801, 1051, 1090, 1134, 1675
Van91I	CCANNNNNTGG	2 cut(s)	1611, 1955
Vha464I	CTTAAG	2 cut(s)	854, 2462
VpaK11BI	GGWCC	3 cut(s)	1946, 2363, 2422
XagI	CCTNNNNNAGG	1 cut(s)	2531
XapI	RAATTY	3 cut(s)	273, 979, 1337
XbaI	TCTAGA	2 cut(s)	626, 1892
XceI	RCATGY	3 cut(s)	419, 2271, 2461
XmiI	GTMKAC	1 cut(s)	2108
XmnI	GAANNNNTTC	1 cut(s)	952
XspI	CTAG	9 cut(s)	284, 627, 911, 984, 1424, 1746, 1893, 2475, 2496
Zsp2I	ATGCAT	1 cut(s)	688

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.