Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0001061

Physical Location & Seq

Genomic Coordinates Forward (+)

75663817 .. 75666648

Gene Structure 2832 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ52814

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1152 bp

ATGCCTGTACGGCCTTCATCAAAGCTCGTCCTTCAGCCTAGTTCAAAGCTCAGGAATCAACAGAGCTCTGTAGAAATGGAGGCCTTCTTGACCCATGTATGCAAGACAAGAACACTGCTTACATTGATCTTATGTATTTGGGTGCCCATAGAAGTGATGGCTCAGACTGGAAATGCCCCCGTCTCGTCTAAGAGGCCGAGTTCTCTGAATATTGGAGCTCTGTTTACTTTCAATTCAGCCATTGGAAGGTCGGCCAAACCAGCAATCCTAGCTGCAATTGAAGAAGTTAATTCTGATTCAAGCGTTCTCGAGGGGACTAAATTGAACATTATTTTCCATGATACAAATTGCAGTGGATTTCTTGGAACTGTGGAAGCTTTGCAGCTCATTGAAAATGATGTGGTTGCTGCAATTGGCCCACAGTCCTCAGGAATAGCTCATGTCATATCCCACGTTGTTAATGAGCTCCATGTACCACTTCTGTCATTTGGAGCAACAGACCCCTCCCTTTCTGCTCTACAATACCCGTATTTTGTACGCACCACGCAGAGTGACTATTTTCAAATGTATGCAGTTGCTGATATGGTTGAGTATTTCGGATGGAGAGAAGTAATTGCCATATTTGTAGATGATGATTGTGGCAGAAATGGGATTTCAGTATTAGGTGATGCCTTAGCAAAGAAGCGTTCCAAGATTGCGTACAAGGCTGCCTTCTCTCCTGGAGCCCCATTAAGCGATATCAATGACTTGTTGGTGGGAGTAAACCTCATGGAATCTCGGGTGTATATTGTACATGTAAATCCTGACTCTGGTTTGTCAATCTTTTCTGTTGCCAAGGATCTTGGAATGATGACCAGTGGCTATGTTTGGATTGCAACAGATTGGCTTCCTACTAATTTAGATTCATTAGTACCACCTGACCCTGACAAAATGAATCTTTTACAAGGGGTTGTTGCTCTTCGTCATCATTCCCCGGATACTGATCTCAAAAAGAGTTTTGCATCTAAATGGAGCAAACTAAAGCATGAAGGGAGTTTGGGCTTCAATTCTTATGCTCTCTATGCCTATGACTCTATTTGGTTAGCAGCCCGTGCCCTTGATGTTTTCTCAATGAAGGAGGGACTGTATCTTTCTCTAGTGACCCAAAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0004888 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0007154 GO:0007165 GO:0008066 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009507 GO:0009536 GO:0009605 GO:0009611 GO:0009612 GO:0009628 GO:0009719 GO:0009987 GO:0010033 GO:0010034 GO:0010243 GO:0015075 GO:0015085 GO:0015267 GO:0015276 GO:0015318 GO:0016020 GO:0019722 GO:0019932 GO:0022803 GO:0022834 GO:0022836 GO:0022838 GO:0022839 GO:0022857 GO:0022890 GO:0023052 GO:0030001 GO:0033554 GO:0034220 GO:0035556 GO:0038023 GO:0042221 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046677 GO:0046873 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055085 GO:0060089 GO:0065007 GO:0070417 GO:0070588 GO:0070838 GO:0070887 GO:0071214 GO:0071229 GO:0071230 GO:0071236 GO:0071260 GO:0071310 GO:0071311 GO:0071417 GO:0071495 GO:0071496 GO:0071944 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:0104004 GO:1901698 GO:1901699 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

383

Amino Acids

41.76

Weight (kDa)

5.85

Isoelectric Point (pI)

47.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peripla_BP_6	PF13458	71 - 366	1.4e-19	Periplasmic binding protein
ANF_receptor	PF01094	86 - 370	9.9e-75	Receptor family ligand binding region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001061)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G05200 AT1G05200 AT1G05200 AT1G05200 AT1G05200 AT1G05200 AT1G05200 AT2G32390 AT2G32390 AT2G32390 AT2G32390 AT2G32390 AT2G32390 AT2G32390
fragaria_vesca	FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291
malus_domestica	MD09G1069900.v1.1 MD09G1070100.v1.1 MD09G1107700.v1.1 MD09G1108100.v1.1 MD17G1096100.v1.1
prunus_persica	Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1
pyrus_communis	pycom09g03000 pycom09g03030 pycom09g03050 pycom09g03070 pycom15g20430 pycom17g09220
rosa_chinensis	RchiOBHm_Chr1g0324131 RchiOBHm_Chr2g0159571 RchiOBHm_Chr2g0159581
rosa_laevigata	RLG00000021167
rosa_multiflora	Rmu_co8397097.1_g000001 Rmu_sc0004966.1_g000017 Rmu_sc0020031.1_g000001
rosa_roxburghii	Rroxscaffold_2G00090390
rosa_rugosa	Rorug02G0476900 Rorug02G0477000 Rorug02G0477100 Rorug02G0477200
rosa_samantha	Rh2AG544200 Rh2BG557000 Rh2CG527200 Rh2DG566000
rosa_wichuraiana	Rw2G044990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	142
AclWI	GGATC	1 cut(s)	846
AcoI	YGGCCR	1 cut(s)	252
AcuI	CTGAAG	1 cut(s)	17
AfaI	GTAC	6 cut(s)	9, 474, 537, 701, 792, 912
AfiI	CCNNNNNNNGG	2 cut(s)	246, 809
AflIII	ACRYGT	1 cut(s)	793
AgsI	TTSAA	8 cut(s)	45, 232, 281, 300, 325, 392, 563, 1045
AjnI	CCWGG	1 cut(s)	718
AluBI	AGCT	9 cut(s)	25, 49, 66, 218, 272, 377, 385, 437, 466
AluI	AGCT	9 cut(s)	25, 49, 66, 218, 272, 377, 385, 437, 466
Alw21I	GWGCWC	3 cut(s)	68, 220, 468
Alw26I	GTCTC	1 cut(s)	187
AlwI	GGATC	1 cut(s)	846
AlwNI	CAGNNNCTG	1 cut(s)	578
Ama87I	CYCGRG	2 cut(s)	308, 777
AoxI	GGCC	5 cut(s)	11, 81, 194, 252, 415
ApeKI	GCWGC	5 cut(s)	272, 382, 407, 707, 1085
AspS9I	GGNCC	1 cut(s)	416
AsuC2I	CCSGG	1 cut(s)	974
AsuHPI	GGTGA	1 cut(s)	677
AvaI	CYCGRG	2 cut(s)	308, 777
AxyI	CCTNAGG	1 cut(s)	427
BaeGI	GKGCMC	2 cut(s)	147, 1096
BanI	GGYRCC	1 cut(s)	142
BanII	GRGCYC	4 cut(s)	68, 220, 468, 727
Bbv12I	GWGCWC	3 cut(s)	68, 220, 468
BbvI	GCAGC	5 cut(s)	259, 394, 394, 694, 1097
BccI	CCATC	2 cut(s)	151, 594
BceAI	ACGGC	1 cut(s)	26
BciT130I	CCWGG	1 cut(s)	720
BciVI	GTATCC	1 cut(s)	970
BcnI	CCSGG	1 cut(s)	974
BcoDI	GTCTC	1 cut(s)	187
BfaI	CTAG	3 cut(s)	39, 269, 1136
BfmI	CTRYAG	1 cut(s)	69
BfuI	GTATCC	1 cut(s)	970
BglI	GCCNNNNNGGC	1 cut(s)	10
BisI	GCNGC	5 cut(s)	273, 383, 408, 708, 1086
BlsI	GCNGC	5 cut(s)	274, 384, 409, 709, 1087
Bme1390I	CCNGG	2 cut(s)	720, 974
BmeT110I	CYCGRG	2 cut(s)	308, 777
BmgT120I	GGNCC	1 cut(s)	416
BmiI	GGNNCC	2 cut(s)	144, 724
BmrFI	CCNGG	2 cut(s)	720, 974
BmsI	GCATC	2 cut(s)	658, 1010
BplI	GAGNNNNNCTC	2 cut(s)	750, 782
BpmI	CTGGAG	1 cut(s)	741
Bpu10I	CCTNAGC	2 cut(s)	50, 673
BpuMI	CCSGG	1 cut(s)	974
BsaBI	GATNNNNATC	1 cut(s)	981
BsaJI	CCNNGG	2 cut(s)	834, 972
BsaXI	ACNNNNNCTCC	2 cut(s)	1109, 1139
Bsc4I	CCNNNNNNNGG	2 cut(s)	246, 809
Bse1I	ACTGG	2 cut(s)	172, 855
Bse21I	CCTNAGG	1 cut(s)	427
Bse8I	GATNNNNATC	1 cut(s)	981
BseBI	CCWGG	1 cut(s)	720
BseDI	CCNNGG	2 cut(s)	834, 972
BseGI	GGATG	1 cut(s)	605
BseJI	GATNNNNATC	1 cut(s)	981
BseLI	CCNNNNNNNGG	2 cut(s)	246, 809
BseMII	CTCAG	3 cut(s)	64, 176, 441
BseNI	ACTGG	2 cut(s)	172, 855
BseSI	GKGCMC	2 cut(s)	147, 1096
BseXI	GCAGC	5 cut(s)	259, 394, 394, 694, 1097
BshFI	GGCC	5 cut(s)	13, 83, 196, 254, 417
BshNI	GGYRCC	1 cut(s)	142
BsiHKAI	GWGCWC	3 cut(s)	68, 220, 468
BsiHKCI	CYCGRG	2 cut(s)	308, 777
BsiSI	CCGG	1 cut(s)	974
BslFI	GGGAC	2 cut(s)	328, 1134
BslI	CCNNNNNNNGG	2 cut(s)	246, 809
BsmAI	GTCTC	1 cut(s)	187
BsmBI	CGTCTC	1 cut(s)	187
BsmFI	GGGAC	2 cut(s)	328, 1134
BsnI	GGCC	5 cut(s)	13, 83, 196, 254, 417
BsoBI	CYCGRG	2 cut(s)	308, 777
Bsp1286I	GDGCHC	6 cut(s)	68, 147, 220, 468, 727, 1096
Bsp1407I	TGTACA	1 cut(s)	790
Bsp143I	GATC	3 cut(s)	126, 838, 982
BspANI	GGCC	5 cut(s)	13, 83, 196, 254, 417
BspCNI	CTCAG	3 cut(s)	63, 175, 440
BspLI	GGNNCC	2 cut(s)	144, 724
BspPI	GGATC	1 cut(s)	846
BspQI	GCTCTTC	1 cut(s)	963
BspT107I	GGYRCC	1 cut(s)	142
BsrGI	TGTACA	1 cut(s)	790
BsrI	ACTGG	2 cut(s)	172, 855
BssECI	CCNNGG	2 cut(s)	834, 972
BssMI	GATC	3 cut(s)	126, 838, 982
BssT1I	CCWWGG	1 cut(s)	834
Bst2UI	CCWGG	1 cut(s)	720
Bst4CI	ACNGT	3 cut(s)	370, 423, 1125
Bst6I	CTCTTC	1 cut(s)	963
BstAPI	GCANNNNNTGC	1 cut(s)	1091
BstAUI	TGTACA	1 cut(s)	790
BstDEI	CTNAG	5 cut(s)	50, 162, 189, 427, 673
BstF5I	GGATG	1 cut(s)	605
BstKTI	GATC	3 cut(s)	129, 841, 985
BstMAI	GTCTC	1 cut(s)	187
BstMBI	GATC	3 cut(s)	126, 838, 982
BstMWI	GCNNNNNNNGC	6 cut(s)	10, 260, 269, 704, 1061, 1091
BstNI	CCWGG	1 cut(s)	720
BstNSI	RCATGY	1 cut(s)	797
BstSCI	CCNGG	2 cut(s)	718, 972
BstSFI	CTRYAG	1 cut(s)	69
BstSLI	GKGCMC	2 cut(s)	147, 1096
BstV1I	GCAGC	5 cut(s)	259, 394, 394, 694, 1097
BstX2I	RGATCY	1 cut(s)	838
BstYI	RGATCY	1 cut(s)	838
Bsu36I	CCTNAGG	1 cut(s)	427
BsuI	GTATCC	1 cut(s)	970
BsuRI	GGCC	5 cut(s)	13, 83, 196, 254, 417
BtsCI	GGATG	1 cut(s)	605
BtsI	GCAGTG	2 cut(s)	113, 358
BtsIMutI	CAGTG	3 cut(s)	113, 358, 862
CaiI	CAGNNNCTG	1 cut(s)	578
Cfr13I	GGNCC	1 cut(s)	416
Csp6I	GTAC	6 cut(s)	8, 473, 536, 700, 791, 911
CviAII	CATG	7 cut(s)	95, 338, 440, 470, 769, 794, 1025
CviQI	GTAC	6 cut(s)	8, 473, 536, 700, 791, 911
DdeI	CTNAG	5 cut(s)	50, 162, 189, 427, 673
DpnI	GATC	3 cut(s)	128, 840, 984
DpnII	GATC	3 cut(s)	126, 838, 982
EaeI	YGGCCR	1 cut(s)	252
Eam1104I	CTCTTC	1 cut(s)	963
EarI	CTCTTC	1 cut(s)	963
Ecl136II	GAGCTC	3 cut(s)	66, 218, 466
Eco130I	CCWWGG	1 cut(s)	834
Eco147I	AGGCCT	1 cut(s)	83
Eco24I	GRGCYC	4 cut(s)	68, 220, 468, 727
Eco32I	GATATC	1 cut(s)	739
Eco53kI	GAGCTC	3 cut(s)	66, 218, 466
Eco57I	CTGAAG	1 cut(s)	17
Eco81I	CCTNAGG	1 cut(s)	427
Eco88I	CYCGRG	2 cut(s)	308, 777
EcoICRI	GAGCTC	3 cut(s)	66, 218, 466
EcoRII	CCWGG	1 cut(s)	718
EcoRV	GATATC	1 cut(s)	739
EcoT14I	CCWWGG	1 cut(s)	834
EcoT38I	GRGCYC	4 cut(s)	68, 220, 468, 727
ErhI	CCWWGG	1 cut(s)	834
Esp3I	CGTCTC	1 cut(s)	187
FaeI	CATG	7 cut(s)	98, 341, 443, 473, 772, 797, 1028
FalI	AAGNNNNNCTT	2 cut(s)	695, 727
FaqI	GGGAC	2 cut(s)	328, 1134
FatI	CATG	7 cut(s)	94, 337, 439, 469, 768, 793, 1024
Fnu4HI	GCNGC	5 cut(s)	273, 383, 408, 708, 1086
FokI	GGATG	1 cut(s)	612
FriOI	GRGCYC	4 cut(s)	68, 220, 468, 727
Fsp4HI	GCNGC	5 cut(s)	273, 383, 408, 708, 1086
FspBI	CTAG	3 cut(s)	39, 269, 1136
GluI	GCNGC	5 cut(s)	273, 383, 408, 708, 1086
GsuI	CTGGAG	1 cut(s)	741
HaeIII	GGCC	5 cut(s)	13, 83, 196, 254, 417
HapII	CCGG	1 cut(s)	974
Hin1II	CATG	7 cut(s)	98, 341, 443, 473, 772, 797, 1028
HindIII	AAGCTT	1 cut(s)	375
HinfI	GANTC	7 cut(s)	55, 296, 773, 806, 902, 934, 1070
HpaII	CCGG	1 cut(s)	974
HphI	GGTGA	1 cut(s)	677
Hpy166II	GTNNAC	2 cut(s)	225, 763
Hpy188I	TCNGA	4 cut(s)	165, 207, 295, 599
Hpy188III	TCNNGA	5 cut(s)	52, 88, 308, 429, 803
Hpy8I	GTNNAC	2 cut(s)	225, 763
HpyAV	CCTTC	7 cut(s)	24, 41, 94, 240, 721, 1022, 1108
HpyCH4III	ACNGT	3 cut(s)	370, 423, 1125
HpyCH4IV	ACGT	1 cut(s)	453
HpyCH4V	TGCA	8 cut(s)	102, 275, 351, 382, 410, 572, 875, 1001
HpyF10VI	GCNNNNNNNGC	6 cut(s)	10, 260, 269, 704, 1061, 1091
HpyF3I	CTNAG	5 cut(s)	50, 162, 189, 427, 673
HpySE526I	ACGT	1 cut(s)	453
Hsp92II	CATG	7 cut(s)	98, 341, 443, 473, 772, 797, 1028
Kzo9I	GATC	3 cut(s)	126, 838, 982
LguI	GCTCTTC	1 cut(s)	963
LmnI	GCTCC	5 cut(s)	215, 471, 491, 722, 1011
Lsp1109I	GCAGC	5 cut(s)	259, 394, 394, 694, 1097
LweI	GCATC	2 cut(s)	658, 1010
MaeI	CTAG	3 cut(s)	39, 269, 1136
MaeII	ACGT	1 cut(s)	453
MaeIII	GTNAC	2 cut(s)	551, 1138
MalI	GATC	3 cut(s)	128, 840, 984
MboI	GATC	3 cut(s)	126, 838, 982
MboII	GAAGA	2 cut(s)	293, 950
MfeI	CAATTG	2 cut(s)	276, 411
MflI	RGATCY	1 cut(s)	838
MhlI	GDGCHC	6 cut(s)	68, 147, 220, 468, 727, 1096
MluCI	AATT	9 cut(s)	232, 276, 289, 320, 346, 411, 612, 895, 1045
MlyI	GAGTC	2 cut(s)	800, 1064
MnlI	CCTC	7 cut(s)	73, 186, 304, 436, 514, 776, 1111
MseI	TTAA	3 cut(s)	288, 459, 731
MslI	CAYNNNNRTG	2 cut(s)	152, 1006
MspI	CCGG	1 cut(s)	974
MspR9I	CCNGG	2 cut(s)	720, 974
MunI	CAATTG	2 cut(s)	276, 411
MvaI	CCWGG	1 cut(s)	720
MwoI	GCNNNNNNNGC	6 cut(s)	10, 260, 269, 704, 1061, 1091
NciI	CCSGG	1 cut(s)	974
NdeII	GATC	3 cut(s)	126, 838, 982
NlaIII	CATG	7 cut(s)	98, 341, 443, 473, 772, 797, 1028
NlaIV	GGNNCC	2 cut(s)	144, 724
NmeAIII	GCCGAG	1 cut(s)	222
NmuCI	GTSAC	2 cut(s)	551, 1138
NspI	RCATGY	1 cut(s)	797
PaeR7I	CTCGAG	1 cut(s)	308
PceI	AGGCCT	1 cut(s)	83
PciI	ACATGT	1 cut(s)	793
PciSI	GCTCTTC	1 cut(s)	963
PfeI	GAWTC	5 cut(s)	55, 296, 773, 902, 934
PfoI	TCCNGGA	1 cut(s)	718
PkrI	GCNGC	5 cut(s)	274, 384, 409, 709, 1087
PleI	GAGTC	2 cut(s)	800, 1064
PpsI	GAGTC	2 cut(s)	800, 1064
PscI	ACATGT	1 cut(s)	793
Psp124BI	GAGCTC	3 cut(s)	68, 220, 468
Psp6I	CCWGG	1 cut(s)	718
PspGI	CCWGG	1 cut(s)	718
PspN4I	GGNNCC	2 cut(s)	144, 724
PspPI	GGNCC	1 cut(s)	416
PsrI	GAACNNNNNNTAC	2 cut(s)	103, 135
PstNI	CAGNNNCTG	1 cut(s)	578
PsuI	RGATCY	1 cut(s)	838
RsaI	GTAC	6 cut(s)	9, 474, 537, 701, 792, 912
RsaNI	GTAC	6 cut(s)	8, 473, 536, 700, 791, 911
RseI	CAYNNNNRTG	2 cut(s)	152, 1006
SacI	GAGCTC	3 cut(s)	68, 220, 468
SapI	GCTCTTC	1 cut(s)	963
SaqAI	TTAA	3 cut(s)	288, 459, 731
SatI	GCNGC	5 cut(s)	273, 383, 408, 708, 1086
Sau3AI	GATC	3 cut(s)	126, 838, 982
Sau96I	GGNCC	1 cut(s)	416
SchI	GAGTC	2 cut(s)	800, 1064
ScrFI	CCNGG	2 cut(s)	720, 974
SduI	GDGCHC	6 cut(s)	68, 147, 220, 468, 727, 1096
SfaNI	GCATC	2 cut(s)	658, 1010
SfcI	CTRYAG	1 cut(s)	69
Sfr274I	CTCGAG	1 cut(s)	308
SlaI	CTCGAG	1 cut(s)	308
SmiMI	CAYNNNNRTG	2 cut(s)	152, 1006
SmlI	CTYRAG	1 cut(s)	308
SmoI	CTYRAG	1 cut(s)	308
Sse9I	AATT	9 cut(s)	232, 276, 289, 320, 346, 411, 612, 895, 1045
SseBI	AGGCCT	1 cut(s)	83
SspI	AATATT	1 cut(s)	211
SspMI	CTAG	3 cut(s)	39, 269, 1136
SstI	GAGCTC	3 cut(s)	68, 220, 468
StuI	AGGCCT	1 cut(s)	83
StyD4I	CCNGG	2 cut(s)	718, 972
StyI	CCWWGG	1 cut(s)	834
TaaI	ACNGT	3 cut(s)	370, 423, 1125
TaiI	ACGT	1 cut(s)	456
TaqI	TCGA	1 cut(s)	309
TasI	AATT	9 cut(s)	232, 276, 289, 320, 346, 411, 612, 895, 1045
TatI	WGTACW	1 cut(s)	790
TfiI	GAWTC	5 cut(s)	55, 296, 773, 902, 934
Tru1I	TTAA	3 cut(s)	288, 459, 731
Tru9I	TTAA	3 cut(s)	288, 459, 731
TscAI	CASTG	3 cut(s)	120, 358, 862
TseFI	GTSAC	2 cut(s)	551, 1138
TseI	GCWGC	5 cut(s)	272, 382, 407, 707, 1085
Tsp45I	GTSAC	2 cut(s)	551, 1138
TspDTI	ATGAA	5 cut(s)	6, 894, 947, 1041, 1127
TspRI	CASTG	3 cut(s)	120, 358, 862
XceI	RCATGY	1 cut(s)	797
XcmI	CCANNNNNNNNNTGG	1 cut(s)	154
XhoI	CTCGAG	1 cut(s)	308
XspI	CTAG	3 cut(s)	39, 269, 1136

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.