Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr9

Orthogroup ID

OG0001061

Physical Location & Seq

Genomic Coordinates Reverse (-)

2248991 .. 2252780

Gene Structure 3790 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom09g03030.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2925 bp

ATGGAGGTATTGCTGATGAATAGGTCTACTGACCATGTATGCAAGACAAGAACACTGCTTGCATTGATCTTGTGCATGTGGGTGTCCATGCAAGTGATGGCTGGAACAGAAAATGCCACGCATTTGTCTGCCAGGCCAAGTTCTCTGAATATTGGAGCTCTGTTTACTTTCAATTCCGTTATTGGGAGGGCAGCCAAGCCGGCAATTTTAGCTGCAATTGATGATGTCAATTCTGATCAAAGCATTCTTCGCGGGACAAAATTGAACATTATTCTCCATGACACGAATTGCAGTGGATTTCTTGGAACTGTTGAAGCTATGGAGCTGATTGAAAACGACGTGGTTGCTGCAGTCGGCCCACAATCCTCTGGAATAGCTCATGTCATATCCCATGTTGTTAATGAGCTCCATGTACCACTTCTATCATTTGGAGCAACAGACCCCTCCCTTGCTGCTCTCCAATTCCCATATTTTGTCCGAACCACACAGAGTGACTATTTCCAAATGTATGCGGTTGCTGATTTAGTTGAGTATTTTGGATGGAGAGAAGTAATAGCAATCTTTGTAGATGATGATTATGGCAGAAACGGGATTTCTATACTAGGCGATGCCTTGGCGGTGAAGCGTTCTAAGATCTCATACAAGGCTGCCTTCTCTCCTGGAGCCCCCAAAAGCGATATCAATGAATTGTTGGTGGGAGTGAACCTCATGGAATCTCGGGTTTATATTGTACATGTTAATCCCGACTCCGGTTTAACTATTTTTTCCATTGCCAAGTCACTTGGAATGATGACCAGTGGCTATGTCTGGATTGCGACGGATTGGCTTCCTTCTCATTTAGATTCACTAAATCCCCCTGGCCCCGACACAATGAATCTCTTACAAGGGGTTGTTGCTCTTCGTCATCATACCCCAGATACTGATCTCAAAAAGCGCTTTATGTCTAGATGGAGCAAATTGCAACATGACGGGAGTCCAAGCTTCAATTCTTATGCGCTCTATGCATACGACTCTATTTGGCTAGCAGCCCGTGCTCTTGATGTTTTCTTCAACGAAGGTGGGAATGTATCTTTCTCTAATGACCGAAGGTTGAATGACACAAATCGAAGCACACTGCATTTAACATCACTCCGTATTTTTGATGGAGGCCAAAAATATCTACAGACGATTCTTAAGATGAACTTCACAGGTATAAGTGGTCAGATTGAGTTTGATCAAGATAAATATCTAGTTCGTCCAGCATATGACATTCTGAATATTGGAGGAACTGGTTCCCGTAGAGTTGGCTATTGGTCAAATTCTACTGGTCTCTCAGTCATTGCTCCAGAGATATTATATAAGAAGCCATTCAATAGGAATACGACTGCTCAACTTTATAGCGTTATATGGCCTGGTGAAGTTACAGCTATACCTCGGGGATGGGTATTTCCCAACAATGGCAGGCCACTGCGAATTGGAGTGCCTTATCGGGTAAGTTACGAAGATTTTGTGGTTAAAGATAAGAGCCCTCCAGGGGTCAGAGGATACTGTATTGATGTCTTTGAAGCTGCTGTAAACTTACTGCCGTATGCTGTGCCACGGACATATATGTTGTATGGAGATGGTAAGAGGAATCCTGAGTACAGCAGTCTTGTAGCCCAGGTTGCGCAAAATAAATTTGATGCAGCTGTTGGAGATATTACAATTACTACTAATAGGACAAGAATAGTTGATTTTACACAGCCTTACATGGAATCGGGACTTGTTGTAGTTGTTCCTGTCAAAGAGGAAAAATCAAACGCTTGGGCTTTCCTCAAGCCATTTACTTACCAGATGTGGTTGGTCACCGGTGCCTTCTTCCTTTTCGTGGGAGCTGTTGTTTGGATTCTTGAGCACCGGATCAATCATGAGTTCCGTGGTCCACCGAGCCAACAGCTCATAACCATTTTTTGGTTTAGTTTCTCCACAATGTTTTTCTCACAGAGAGAGAACACTGTGAGCACCCTGGGACGGCTGGTGCTGATTATATGGTTATTTGTAGTGTTAATTATCAATTCCAGCTACACGGCTAGTTTGACTTCAATTCTCACGGTGCAACGGCTGACGTCACGGATTGAAGGGATTGACAACTTGATATCAAGTAATGATCGAATCGGAGTTCAAGACGGGTCATTCGTTTGGAGGTATTTGGTTGATGAGATGAACATAGCGGAATCTAGACTGGTTAAGTTGGAAGACATGGAAGCCTATATTAAAGCCCTTACAGATGGACCGACACGTGGTGGGGTAGCTGCCATCGTTGATGAGCTTCCTTACATTGAGTTGTTTATGTCCAGCACCAAATGCGCATTCAGGACAGTAGGGCCGGAGTTTAGCAAAAGCGGATGGGGATTTGCCTTCCAAAAGGATTCTCCTCTTGCTGTTGACTTGTCGACTGCAATTCTTCAACTCTCAGAGAACGGTGATCTGCAAAAGATCCGTAATAAGTGGCTTACGCATAACGAGTGCCCTACTCAACTCAATAAAGTTGATGGAGGCCAGCTATCTCTAACAAGCTTTTGGGGCCTGTTTCTTATCTGCGGTATTGCATGCTTCCTTGCTCTTGCGGTGTTCTTCTGTAGGATCCTTTGTCAATACCGCAGGTTTACCCCCGAGCCTGTGGAAGCGGACGTTGAGGAGATTGGACCTACCAACACGAGATCTCGACGCTTACTCTGGTCAACTATTTTTAAGATCGATCTTATGGTCTTTGTAGATAAGAAAGAAGCAGAGATAAAGCACATGCTTAAGCGAAAGACTAGTGATAGTAAGCACGAAGCTAGCCCGAGCGCTGATGGGCGGCTGCATTCCCTTCGTGAGGAAGATTTGAGCGCTGATGGGCAGCTGCATTCCCTTCGTGAGGAAGATTTGAGAGCTGATGGGCGGCTGCATTCCCTTCGTACGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0004888 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0007154 GO:0007165 GO:0008066 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009507 GO:0009536 GO:0009605 GO:0009611 GO:0009612 GO:0009628 GO:0009719 GO:0009987 GO:0010033 GO:0010034 GO:0010243 GO:0015075 GO:0015085 GO:0015267 GO:0015276 GO:0015318 GO:0016020 GO:0019722 GO:0019932 GO:0022803 GO:0022834 GO:0022836 GO:0022838 GO:0022839 GO:0022857 GO:0022890 GO:0023052 GO:0030001 GO:0033554 GO:0034220 GO:0035556 GO:0038023 GO:0042221 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046677 GO:0046873 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055085 GO:0060089 GO:0065007 GO:0070417 GO:0070588 GO:0070838 GO:0070887 GO:0071214 GO:0071229 GO:0071230 GO:0071236 GO:0071260 GO:0071310 GO:0071311 GO:0071417 GO:0071495 GO:0071496 GO:0071944 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:0104004 GO:1901698 GO:1901699 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

975

Amino Acids

108.96

Weight (kDa)

6.4

Isoelectric Point (pI)

41.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peripla_BP_6	PF13458	51 - 346	2.8e-18	Periplasmic binding protein
ANF_receptor	PF01094	65 - 422	2.8e-90	Receptor family ligand binding region
Lig_chan-Glu_bd	PF10613	499 - 587	6.2e-12	Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3	PF00497	540 - 825	3.5e-13	Bacterial extracellular solute-binding proteins, family 3
Lig_chan	PF00060	603 - 857	4.7e-39	Ligand-gated ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001061)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G05200 AT1G05200 AT1G05200 AT1G05200 AT1G05200 AT1G05200 AT1G05200 AT2G32390 AT2G32390 AT2G32390 AT2G32390 AT2G32390 AT2G32390 AT2G32390
fragaria_vesca	FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291
malus_domestica	MD09G1069900.v1.1 MD09G1070100.v1.1 MD09G1107700.v1.1 MD09G1108100.v1.1 MD17G1096100.v1.1
prunus_persica	Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1
pyrus_communis	pycom09g03000 pycom09g03030 pycom09g03050 pycom09g03070 pycom15g20430 pycom17g09220
rosa_chinensis	RchiOBHm_Chr1g0324131 RchiOBHm_Chr2g0159571 RchiOBHm_Chr2g0159581
rosa_laevigata	RLG00000021167
rosa_multiflora	Rmu_co8397097.1_g000001 Rmu_sc0004966.1_g000017 Rmu_sc0020031.1_g000001
rosa_roxburghii	Rroxscaffold_2G00090390
rosa_rugosa	Rorug02G0476900 Rorug02G0477000 Rorug02G0477100 Rorug02G0477200
rosa_samantha	Rh2AG544200 Rh2BG557000 Rh2CG527200 Rh2DG566000
rosa_wichuraiana	Rw2G044990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	2087
Acc16I	TGCGCA	2 cut(s)	1647, 2326
Acc36I	ACCTGC	1 cut(s)	2610
AccB1I	GGYRCC	1 cut(s)	1829
AccB7I	CCANNNNNTGG	1 cut(s)	1929
AccI	GTMKAC	2 cut(s)	26, 2411
AccII	CGCG	1 cut(s)	252
AclWI	GGATC	4 cut(s)	1886, 2449, 2596, 2609
AcsI	RAATTY	2 cut(s)	1297, 1655
AcvI	CACGTG	1 cut(s)	2258
AcyI	GRCGYC	1 cut(s)	2084
AdeI	CACNNNGTG	2 cut(s)	491, 2261
AfaI	GTAC	4 cut(s)	414, 732, 1622, 2920
AfeI	AGCGCT	3 cut(s)	935, 2809, 2851
AflII	CTTAAG	2 cut(s)	1172, 2765
AflIII	ACRYGT	2 cut(s)	733, 2255
AgeI	ACCGGT	1 cut(s)	1826
AhlI	ACTAGT	1 cut(s)	2777
AjiI	CACGTC	1 cut(s)	340
AjnI	CCWGG	7 cut(s)	131, 658, 856, 1390, 1510, 1638, 1983
AjuI	GAANNNNNNNTTGG	4 cut(s)	970, 1002, 2312, 2344
Alw21I	GWGCWC	5 cut(s)	160, 408, 1036, 1875, 1982
Alw26I	GTCTC	1 cut(s)	1313
AlwI	GGATC	4 cut(s)	1886, 2449, 2596, 2609
AlwNI	CAGNNNCTG	1 cut(s)	920
Ama87I	CYCGRG	4 cut(s)	717, 1413, 2630, 2803
Aor51HI	AGCGCT	3 cut(s)	935, 2809, 2851
AoxI	GGCC	9 cut(s)	134, 355, 859, 1147, 1388, 1442, 2342, 2515, 2542
ApoI	RAATTY	2 cut(s)	1297, 1655
ArsI	GACNNNNNNTTYG	2 cut(s)	2135, 2167
AsiGI	ACCGGT	1 cut(s)	1826
Asp700I	GAANNNNTTC	2 cut(s)	1270, 1346
AspLEI	GCGC	6 cut(s)	936, 997, 1648, 2327, 2810, 2852
AspS9I	GGNCC	7 cut(s)	356, 860, 1898, 2249, 2342, 2542, 2663
AsuHPI	GGTGA	4 cut(s)	631, 1406, 1816, 2453
AsuNHI	GCTAGC	2 cut(s)	1021, 2798
AvaI	CYCGRG	4 cut(s)	717, 1413, 2630, 2803
AvaII	GGWCC	3 cut(s)	1898, 2249, 2663
BaeGI	GKGCMC	1 cut(s)	2489
BamHI	GGATCC	1 cut(s)	2601
BanI	GGYRCC	1 cut(s)	1829
BanII	GRGCYC	4 cut(s)	160, 408, 667, 1508
BauI	CACGAG	1 cut(s)	2674
BbrPI	CACGTG	1 cut(s)	2258
BbsI	GAAGAC	1 cut(s)	2220
Bbv12I	GWGCWC	5 cut(s)	160, 408, 1036, 1875, 1982
BceAI	ACGGC	4 cut(s)	1549, 2006, 2061, 2093
BcgI	CGANNNNNNTGC	7 cut(s)	326, 360, 2813, 2847, 2855, 2889, 2897
BciT130I	CCWGG	7 cut(s)	133, 660, 858, 1392, 1512, 1640, 1985
BciVI	GTATCC	1 cut(s)	1517
BclI	TGATCA	2 cut(s)	235, 1213
BcoDI	GTCTC	1 cut(s)	1313
BcuI	ACTAGT	1 cut(s)	2777
BfaI	CTAG	8 cut(s)	602, 945, 1022, 1229, 2049, 2196, 2778, 2799
BfmI	CTRYAG	3 cut(s)	348, 1160, 2596
BfoI	RGCGCY	3 cut(s)	937, 2811, 2853
BfrI	CTTAAG	2 cut(s)	1172, 2765
BfuAI	ACCTGC	1 cut(s)	2610
BfuI	GTATCC	1 cut(s)	1517
BglI	GCCNNNNNGGC	1 cut(s)	200
BglII	AGATCT	2 cut(s)	633, 2678
Bme1390I	CCNGG	7 cut(s)	133, 660, 858, 1392, 1512, 1640, 1985
Bme18I	GGWCC	3 cut(s)	1898, 2249, 2663
BmeT110I	CYCGRG	4 cut(s)	717, 1413, 2630, 2803
BmgBI	CACGTC	1 cut(s)	340
BmgT120I	GGNCC	7 cut(s)	356, 860, 1898, 2249, 2342, 2542, 2663
BmiI	GGNNCC	6 cut(s)	664, 862, 1273, 1831, 2543, 2603
BmrFI	CCNGG	7 cut(s)	133, 660, 858, 1392, 1512, 1640, 1985
BmsI	GCATC	2 cut(s)	598, 1651
BmtI	GCTAGC	2 cut(s)	1025, 2802
BoxI	GACNNNNGTC	1 cut(s)	972
BpiI	GAAGAC	1 cut(s)	2220
BplI	GAGNNNNNCTC	2 cut(s)	690, 722
BpmI	CTGGAG	3 cut(s)	681, 1308, 1494
BpuEI	CTTGAG	2 cut(s)	1778, 1889
Bsa29I	ATCGAT	1 cut(s)	2715
BsaAI	YACGTR	2 cut(s)	2258, 2922
BsaBI	GATNNNNATC	2 cut(s)	921, 1224
BsaHI	GRCGYC	1 cut(s)	2084
BsaI	GGTCTC	1 cut(s)	1313
BsaJI	CCNNGG	9 cut(s)	612, 856, 1412, 1511, 1577, 1638, 1894, 1983, 1984
BsaWI	WCCGGW	3 cut(s)	749, 1826, 1875
BsaXI	ACNNNNNCTCC	2 cut(s)	1842, 1872
Bse118I	RCCGGY	2 cut(s)	199, 1826
Bse1I	ACTGG	4 cut(s)	795, 1273, 1309, 2205
Bse3DI	GCAATG	2 cut(s)	768, 1317
Bse8I	GATNNNNATC	2 cut(s)	921, 1224
BseBI	CCWGG	7 cut(s)	133, 660, 858, 1392, 1512, 1640, 1985
BseCI	ATCGAT	1 cut(s)	2715
BseDI	CCNNGG	9 cut(s)	612, 856, 1412, 1511, 1577, 1638, 1894, 1983, 1984
BseGI	GGATG	3 cut(s)	545, 1424, 2369
BseJI	GATNNNNATC	2 cut(s)	921, 1224
BseMI	GCAATG	2 cut(s)	768, 1317
BseMII	CTCAG	3 cut(s)	1326, 1608, 2445
BseNI	ACTGG	4 cut(s)	795, 1273, 1309, 2205
BseRI	GAGGAG	2 cut(s)	2382, 2669
BseSI	GKGCMC	1 cut(s)	2489
Bsh1236I	CGCG	1 cut(s)	252
BshFI	GGCC	9 cut(s)	136, 357, 861, 1149, 1390, 1444, 2344, 2517, 2544
BshNI	GGYRCC	1 cut(s)	1829
BshTI	ACCGGT	1 cut(s)	1826
BshVI	ATCGAT	1 cut(s)	2715
BsiHKAI	GWGCWC	5 cut(s)	160, 408, 1036, 1875, 1982
BsiHKCI	CYCGRG	4 cut(s)	717, 1413, 2630, 2803
BsiSI	CCGG	5 cut(s)	200, 750, 1827, 1876, 2345
BsiWI	CGTACG	1 cut(s)	2918
BslFI	GGGAC	3 cut(s)	268, 1752, 2001
BsmAI	GTCTC	1 cut(s)	1313
BsmFI	GGGAC	3 cut(s)	268, 1752, 2001
BsmI	GAATGC	5 cut(s)	243, 2327, 2824, 2866, 2908
BsnI	GGCC	9 cut(s)	136, 357, 861, 1149, 1390, 1444, 2344, 2517, 2544
Bso31I	GGTCTC	1 cut(s)	1313
BsoBI	CYCGRG	4 cut(s)	717, 1413, 2630, 2803
Bsp1286I	GDGCHC	8 cut(s)	160, 408, 667, 1036, 1508, 1875, 1982, 2489
Bsp1407I	TGTACA	1 cut(s)	730
BspANI	GGCC	9 cut(s)	136, 357, 861, 1149, 1390, 1444, 2344, 2517, 2544
BspCNI	CTCAG	3 cut(s)	1325, 1609, 2444
BspDI	ATCGAT	1 cut(s)	2715
BspFNI	CGCG	1 cut(s)	252
BspHI	TCATGA	1 cut(s)	1885
BspLI	GGNNCC	6 cut(s)	664, 862, 1273, 1831, 2543, 2603
BspMAI	CTGCAG	1 cut(s)	352
BspMI	ACCTGC	1 cut(s)	2610
BspOI	GCTAGC	2 cut(s)	1025, 2802
BspPI	GGATC	4 cut(s)	1886, 2449, 2596, 2609
BspQI	GCTCTTC	1 cut(s)	903
BspT107I	GGYRCC	1 cut(s)	1829
BspTI	CTTAAG	2 cut(s)	1172, 2765
BspTNI	GGTCTC	1 cut(s)	1313
BsrDI	GCAATG	2 cut(s)	768, 1317
BsrFI	RCCGGY	2 cut(s)	199, 1826
BsrGI	TGTACA	1 cut(s)	730
BsrI	ACTGG	4 cut(s)	795, 1273, 1309, 2205
BssAI	RCCGGY	2 cut(s)	199, 1826
BssECI	CCNNGG	9 cut(s)	612, 856, 1412, 1511, 1577, 1638, 1894, 1983, 1984
BssNI	GRCGYC	1 cut(s)	2084
BssSI	CACGAG	1 cut(s)	2674
BssT1I	CCWWGG	1 cut(s)	612
Bst2BI	CACGAG	1 cut(s)	2674
Bst2UI	CCWGG	7 cut(s)	133, 660, 858, 1392, 1512, 1640, 1985
Bst4CI	ACNGT	6 cut(s)	310, 1529, 1975, 2071, 2338, 2441
Bst6I	CTCTTC	1 cut(s)	903
BstACI	GRCGYC	1 cut(s)	2084
BstAFI	CTTAAG	2 cut(s)	1172, 2765
BstAPI	GCANNNNNTGC	2 cut(s)	1031, 2322
BstAUI	TGTACA	1 cut(s)	730
BstBAI	YACGTR	2 cut(s)	2258, 2922
BstC8I	GCNNGC	7 cut(s)	60, 201, 1023, 1442, 2519, 2569, 2800
BstDEI	CTNAG	4 cut(s)	630, 1312, 1617, 2431
BstDSI	CCRYGG	2 cut(s)	1577, 1894
BstEII	GGTNACC	1 cut(s)	1822
BstENI	CCTNNNNNAGG	2 cut(s)	2834, 2876
BstF5I	GGATG	3 cut(s)	545, 1424, 2369
BstFNI	CGCG	1 cut(s)	252
BstH2I	RGCGCY	3 cut(s)	937, 2811, 2853
BstHHI	GCGC	6 cut(s)	936, 997, 1648, 2327, 2810, 2852
BstMAI	GTCTC	1 cut(s)	1313
BstMWI	GCNNNNNNNGC	8 cut(s)	200, 209, 249, 1001, 1031, 1643, 2322, 2541
BstNI	CCWGG	7 cut(s)	133, 660, 858, 1392, 1512, 1640, 1985
BstNSI	RCATGY	4 cut(s)	79, 737, 2571, 2764
BstPAI	GACNNNNGTC	1 cut(s)	972
BstPI	GGTNACC	1 cut(s)	1822
BstSCI	CCNGG	7 cut(s)	131, 658, 856, 1390, 1510, 1638, 1983
BstSFI	CTRYAG	3 cut(s)	348, 1160, 2596
BstSLI	GKGCMC	1 cut(s)	2489
BstUI	CGCG	1 cut(s)	252
BstV2I	GAAGAC	1 cut(s)	2220
BstX2I	RGATCY	4 cut(s)	633, 2454, 2601, 2678
BstYI	RGATCY	4 cut(s)	633, 2454, 2601, 2678
Bsu15I	ATCGAT	1 cut(s)	2715
BsuI	GTATCC	1 cut(s)	1517
BsuRI	GGCC	9 cut(s)	136, 357, 861, 1149, 1390, 1444, 2344, 2517, 2544
BsuTUI	ATCGAT	1 cut(s)	2715
BtgI	CCRYGG	2 cut(s)	1577, 1894
BtgZI	GCGATG	1 cut(s)	621
BtrI	CACGTC	1 cut(s)	340
BtsCI	GGATG	3 cut(s)	545, 1424, 2369
BtsI	GCAGTG	4 cut(s)	53, 298, 1112, 1445
BtsIMutI	CAGTG	6 cut(s)	53, 298, 802, 1112, 1445, 1971
BveI	ACCTGC	1 cut(s)	2610
Cac8I	GCNNGC	7 cut(s)	60, 201, 1023, 1442, 2519, 2569, 2800
CaiI	CAGNNNCTG	1 cut(s)	920
CciI	TCATGA	1 cut(s)	1885
CfoI	GCGC	6 cut(s)	936, 997, 1648, 2327, 2810, 2852
Cfr10I	RCCGGY	2 cut(s)	199, 1826
Cfr13I	GGNCC	7 cut(s)	356, 860, 1898, 2249, 2342, 2542, 2663
ClaI	ATCGAT	1 cut(s)	2715
CseI	GACGC	1 cut(s)	2694
Csp6I	GTAC	4 cut(s)	413, 731, 1621, 2919
CspAI	ACCGGT	1 cut(s)	1826
CspCI	CAANNNNNGTGG	2 cut(s)	1039, 1074
CviQI	GTAC	4 cut(s)	413, 731, 1621, 2919
DdeI	CTNAG	4 cut(s)	630, 1312, 1617, 2431
DraIII	CACNNNGTG	2 cut(s)	491, 2261
Eam1104I	CTCTTC	1 cut(s)	903
EarI	CTCTTC	1 cut(s)	903
Ecl136II	GAGCTC	2 cut(s)	158, 406
Eco130I	CCWWGG	1 cut(s)	612
Eco24I	GRGCYC	4 cut(s)	160, 408, 667, 1508
Eco31I	GGTCTC	1 cut(s)	1313
Eco32I	GATATC	2 cut(s)	679, 2115
Eco47I	GGWCC	3 cut(s)	1898, 2249, 2663
Eco47III	AGCGCT	3 cut(s)	935, 2809, 2851
Eco53kI	GAGCTC	2 cut(s)	158, 406
Eco72I	CACGTG	1 cut(s)	2258
Eco88I	CYCGRG	4 cut(s)	717, 1413, 2630, 2803
Eco91I	GGTNACC	1 cut(s)	1822
EcoICRI	GAGCTC	2 cut(s)	158, 406
EcoNI	CCTNNNNNAGG	2 cut(s)	2834, 2876
EcoO109I	RGGNCCY	1 cut(s)	2542
EcoO65I	GGTNACC	1 cut(s)	1822
EcoRII	CCWGG	7 cut(s)	131, 658, 856, 1390, 1510, 1638, 1983
EcoRV	GATATC	2 cut(s)	679, 2115
EcoT14I	CCWWGG	1 cut(s)	612
EcoT22I	ATGCAT	1 cut(s)	1006
EcoT38I	GRGCYC	4 cut(s)	160, 408, 667, 1508
ErhI	CCWWGG	1 cut(s)	612
FalI	AAGNNNNNCTT	4 cut(s)	635, 667, 1166, 1198
FaqI	GGGAC	3 cut(s)	268, 1752, 2001
FauI	CCCGC	1 cut(s)	245
FauNDI	CATATG	1 cut(s)	1243
FbaI	TGATCA	2 cut(s)	235, 1213
FblI	GTMKAC	2 cut(s)	26, 2411
FokI	GGATG	3 cut(s)	552, 1431, 2376
FriOI	GRGCYC	4 cut(s)	160, 408, 667, 1508
FspAI	RTGCGCAY	1 cut(s)	2326
FspBI	CTAG	8 cut(s)	602, 945, 1022, 1229, 2049, 2196, 2778, 2799
FspI	TGCGCA	2 cut(s)	1647, 2326
GlaI	GCGC	6 cut(s)	935, 996, 1647, 2326, 2809, 2851
GsuI	CTGGAG	3 cut(s)	681, 1308, 1494
HaeII	RGCGCY	3 cut(s)	937, 2811, 2853
HaeIII	GGCC	9 cut(s)	136, 357, 861, 1149, 1390, 1444, 2344, 2517, 2544
HapII	CCGG	5 cut(s)	200, 750, 1827, 1876, 2345
HgaI	GACGC	1 cut(s)	2694
HhaI	GCGC	6 cut(s)	936, 997, 1648, 2327, 2810, 2852
Hin1I	GRCGYC	1 cut(s)	2084
Hin6I	GCGC	6 cut(s)	934, 995, 1646, 2325, 2808, 2850
HinP1I	GCGC	6 cut(s)	934, 995, 1646, 2325, 2808, 2850
HincII	GTYRAC	3 cut(s)	2404, 2412, 2700
HindII	GTYRAC	3 cut(s)	2404, 2412, 2700
HindIII	AAGCTT	2 cut(s)	979, 2533
HpaII	CCGG	5 cut(s)	200, 750, 1827, 1876, 2345
HphI	GGTGA	4 cut(s)	631, 1406, 1816, 2453
Hpy166II	GTNNAC	9 cut(s)	27, 165, 703, 1555, 1901, 2404, 2412, 2625, 2700
Hpy188I	TCNGA	8 cut(s)	147, 235, 479, 1203, 1254, 1520, 2135, 2434
Hpy8I	GTNNAC	9 cut(s)	27, 165, 703, 1555, 1901, 2404, 2412, 2625, 2700
Hpy99I	CGWCG	3 cut(s)	341, 820, 2688
HpyCH4III	ACNGT	6 cut(s)	310, 1529, 1975, 2071, 2338, 2441
HpyCH4IV	ACGT	5 cut(s)	339, 2084, 2257, 2649, 2921
HpyF10VI	GCNNNNNNNGC	8 cut(s)	200, 209, 249, 1001, 1031, 1643, 2322, 2541
HpyF3I	CTNAG	4 cut(s)	630, 1312, 1617, 2431
HpySE526I	ACGT	5 cut(s)	339, 2084, 2257, 2649, 2921
Hsp92I	GRCGYC	1 cut(s)	2084
HspAI	GCGC	6 cut(s)	934, 995, 1646, 2325, 2808, 2850
KroI	GCCGGC	1 cut(s)	199
KroNI	GCCGGC	1 cut(s)	201
Ksp22I	TGATCA	2 cut(s)	235, 1213
LguI	GCTCTTC	1 cut(s)	903
LmnI	GCTCC	8 cut(s)	155, 322, 411, 431, 662, 951, 1327, 1850
LweI	GCATC	2 cut(s)	598, 1651
MaeI	CTAG	8 cut(s)	602, 945, 1022, 1229, 2049, 2196, 2778, 2799
MaeII	ACGT	5 cut(s)	339, 2084, 2257, 2649, 2921
MaeIII	GTNAC	6 cut(s)	491, 777, 1399, 1475, 1822, 2085
MfeI	CAATTG	1 cut(s)	216
MflI	RGATCY	4 cut(s)	633, 2454, 2601, 2678
MhlI	GDGCHC	8 cut(s)	160, 408, 667, 1036, 1508, 1875, 1982, 2489
MlyI	GAGTC	3 cut(s)	740, 982, 1004
MmeI	TCCRAC	2 cut(s)	1651, 2190
Mph1103I	ATGCAT	1 cut(s)	1006
MroNI	GCCGGC	1 cut(s)	199
MroXI	GAANNNNTTC	2 cut(s)	1270, 1346
MslI	CAYNNNNRTG	2 cut(s)	80, 92
MspA1I	CMGCKG	2 cut(s)	1667, 2863
MspCI	CTTAAG	2 cut(s)	1172, 2765
MspI	CCGG	5 cut(s)	200, 750, 1827, 1876, 2345
MspR9I	CCNGG	7 cut(s)	133, 660, 858, 1392, 1512, 1640, 1985
MunI	CAATTG	1 cut(s)	216
Mva1269I	GAATGC	5 cut(s)	243, 2327, 2824, 2866, 2908
MvaI	CCWGG	7 cut(s)	133, 660, 858, 1392, 1512, 1640, 1985
MvnI	CGCG	1 cut(s)	252
MwoI	GCNNNNNNNGC	8 cut(s)	200, 209, 249, 1001, 1031, 1643, 2322, 2541
NaeI	GCCGGC	1 cut(s)	201
NdeI	CATATG	1 cut(s)	1243
NgoMIV	GCCGGC	1 cut(s)	199
NheI	GCTAGC	2 cut(s)	1021, 2798
NlaIV	GGNNCC	6 cut(s)	664, 862, 1273, 1831, 2543, 2603
NmuCI	GTSAC	4 cut(s)	491, 777, 1822, 2085
NsbI	TGCGCA	2 cut(s)	1647, 2326
NsiI	ATGCAT	1 cut(s)	1006
NspI	RCATGY	4 cut(s)	79, 737, 2571, 2764
PaeI	GCATGC	1 cut(s)	2571
PagI	TCATGA	1 cut(s)	1885
PasI	CCCWGGG	1 cut(s)	1984
PciI	ACATGT	1 cut(s)	733
PciSI	GCTCTTC	1 cut(s)	903
PcsI	WCGNNNNNNNCGW	1 cut(s)	2151
PctI	GAATGC	5 cut(s)	243, 2327, 2824, 2866, 2908
PdiI	GCCGGC	1 cut(s)	201
PdmI	GAANNNNTTC	2 cut(s)	1270, 1346
Pfl23II	CGTACG	1 cut(s)	2918
PflMI	CCANNNNNTGG	1 cut(s)	1929
PfoI	TCCNGGA	1 cut(s)	658
PinAI	ACCGGT	1 cut(s)	1826
PleI	GAGTC	3 cut(s)	740, 981, 1004
PmaCI	CACGTG	1 cut(s)	2258
PmlI	CACGTG	1 cut(s)	2258
PpsI	GAGTC	3 cut(s)	740, 981, 1004
Ppu21I	YACGTR	2 cut(s)	2258, 2922
PscI	ACATGT	1 cut(s)	733
PshAI	GACNNNNGTC	1 cut(s)	972
Psp124BI	GAGCTC	2 cut(s)	160, 408
Psp6I	CCWGG	7 cut(s)	131, 658, 856, 1390, 1510, 1638, 1983
PspCI	CACGTG	1 cut(s)	2258
PspEI	GGTNACC	1 cut(s)	1822
PspGI	CCWGG	7 cut(s)	131, 658, 856, 1390, 1510, 1638, 1983
PspLI	CGTACG	1 cut(s)	2918
PspN4I	GGNNCC	6 cut(s)	664, 862, 1273, 1831, 2543, 2603
PspPI	GGNCC	7 cut(s)	356, 860, 1898, 2249, 2342, 2542, 2663
PstI	CTGCAG	1 cut(s)	352
PstNI	CAGNNNCTG	1 cut(s)	920
PsuI	RGATCY	4 cut(s)	633, 2454, 2601, 2678
PvuII	CAGCTG	2 cut(s)	1667, 2863
RsaI	GTAC	4 cut(s)	414, 732, 1622, 2920
RsaNI	GTAC	4 cut(s)	413, 731, 1621, 2919
RseI	CAYNNNNRTG	2 cut(s)	80, 92
SacI	GAGCTC	2 cut(s)	160, 408
SalI	GTCGAC	1 cut(s)	2410
SapI	GCTCTTC	1 cut(s)	903
Sau96I	GGNCC	7 cut(s)	356, 860, 1898, 2249, 2342, 2542, 2663
SchI	GAGTC	3 cut(s)	740, 982, 1004
ScrFI	CCNGG	7 cut(s)	133, 660, 858, 1392, 1512, 1640, 1985
SduI	GDGCHC	8 cut(s)	160, 408, 667, 1036, 1508, 1875, 1982, 2489
SfaNI	GCATC	2 cut(s)	598, 1651
SfcI	CTRYAG	3 cut(s)	348, 1160, 2596
SgrAI	CRCCGGYG	1 cut(s)	1826
SinI	GGWCC	3 cut(s)	1898, 2249, 2663
SmiMI	CAYNNNNRTG	2 cut(s)	80, 92
SmlI	CTYRAG	4 cut(s)	1172, 1793, 1868, 2765
SmoI	CTYRAG	4 cut(s)	1172, 1793, 1868, 2765
SpeI	ACTAGT	1 cut(s)	2777
SphI	GCATGC	1 cut(s)	2571
SspI	AATATT	2 cut(s)	151, 1258
SspMI	CTAG	8 cut(s)	602, 945, 1022, 1229, 2049, 2196, 2778, 2799
SstI	GAGCTC	2 cut(s)	160, 408
StyD4I	CCNGG	7 cut(s)	131, 658, 856, 1390, 1510, 1638, 1983
StyI	CCWWGG	1 cut(s)	612
TaaI	ACNGT	6 cut(s)	310, 1529, 1975, 2071, 2338, 2441
TaiI	ACGT	5 cut(s)	342, 2087, 2260, 2652, 2924
TaqI	TCGA	5 cut(s)	1105, 2128, 2411, 2683, 2715
TaqII	GACCGA	2 cut(s)	1098, 2266
TatI	WGTACW	2 cut(s)	730, 1620
TauI	GCSGC	2 cut(s)	2821, 2905
TscAI	CASTG	6 cut(s)	60, 298, 802, 1119, 1452, 1978
TseFI	GTSAC	4 cut(s)	491, 777, 1822, 2085
Tsp45I	GTSAC	4 cut(s)	491, 777, 1822, 2085
TspDTI	ATGAA	5 cut(s)	32, 699, 887, 1193, 2195
TspGWI	ACGGA	7 cut(s)	166, 833, 1121, 1594, 1883, 2104, 2447
TspRI	CASTG	6 cut(s)	60, 298, 802, 1119, 1452, 1978
Van91I	CCANNNNNTGG	1 cut(s)	1929
Vha464I	CTTAAG	2 cut(s)	1172, 2765
VpaK11BI	GGWCC	3 cut(s)	1898, 2249, 2663
XagI	CCTNNNNNAGG	2 cut(s)	2834, 2876
XapI	RAATTY	2 cut(s)	1297, 1655
XbaI	TCTAGA	2 cut(s)	944, 2195
XceI	RCATGY	4 cut(s)	79, 737, 2571, 2764
XcmI	CCANNNNNNNNNTGG	1 cut(s)	94
XmiI	GTMKAC	2 cut(s)	26, 2411
XmnI	GAANNNNTTC	2 cut(s)	1270, 1346
XspI	CTAG	8 cut(s)	602, 945, 1022, 1229, 2049, 2196, 2778, 2799
ZraI	GACGTC	1 cut(s)	2085
Zsp2I	ATGCAT	1 cut(s)	1006

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.