Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0001061

Physical Location & Seq

Genomic Coordinates Forward (+)

72513557 .. 72517949

Gene Structure 4393 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000021167

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2448 bp

ATGGCTCAGACTGGAAATGCCACCGTCTCGTCTAAGAGGCCGAGTTCTCTGAATATTGGAGCTCTATTTACTTTCAATTCAGCCATTGGAAGGTCGGCCAAACCAGCAATCCTAGCCGCAATTGAAGAAGTTAATTCTGATTCAAGTGTTCTCGAGGGGACTAAATTGAACATTATTTTCCATGATACAAATTGCAGTGGATTTCTTGGAACTGTGGAAGCTTTGCAGCTCATTGAAAATGATGTGGTTGCTGCAATTGGCCCACAGTCCTCAGGAATAGCTCATGTCATATCCCACGTTGTTAATGAGCTCCATGTACCACTTCTGTCATTTGGAGCAACAGACCCCTCCCTTTCTGCTCTACAATACCCGTATTTTGTACGCACCACGCAGAGTGACTATTTTCAAATGTATGCAGTTGCTGATATGGTTGAGTATTTCGGATGGAGAGAAGTAATTGCCATATTTGTAGATGATGATTGTGGCAGAAATGGGATTTCAGTACTAGGTGATGCCTTAGCAAAGAAGCGTTCCAAGATTGCTTATAAGGCTGCCTTCTCTCCTGGAGCCCCATTAAGCGATATCAATGACTTGTTGGTGGGAGTAAACCTCATGGAATCTCGGGTGTATATTGTACATGTAAATCCTGACTCTGGTTTGTCAATCTTTTCTGTTGCCAAGGATCTTGGAATGATGACCAGTGGCTATGTTTGGATTGCAACAGATTGGCTTCCTACTAATTTAGATTCATTAGTACCACCTGACCCTGACAAAATGAATCTTTTACAAGGGGTTGTTGCTCTTCGTCATCATTCCCCGGATACTGATCTCAAAAAGAGTTTTGCATCTAAATGGAGCAAAATAAAGCATGAAGGGAGTTCGGGCTTCAATTCTTATGCTCTCTATGCATATGACTCTATTTGGTTAGCAGCCCGTGCCCTTGATGTTTTTCTCAATGAAGGAGGGACTGTATCTTTCTCTAGTGACCCAAAGTTGAAAGATACAAATAGAAGCGCACTGCACTTAGCATCACTCCGAATATTTGATGGAGGCCAACGTTACCTGCAGACACTTCTTAAGATGAACTTCGAAGGTGTGAGTGGTCAGCTTCAGTTTGATCTGGATAAGAATTTAATTCATCCAGCTTATGATGTTCTGAACATTGGTGGAACTGGATTTCGTAAAATTGGTTATTGGTCAAATTCTTCTGGTTTGTCGACCACCGAGCCAGAGACATTATATGGGAAGTCGTTCAATACTTCTACCAGAACTCCACATCTTTATAGTGTTATATGGCCTGGCGAAACTATAGCTACACCTCGGGGATGGGTATTTCCTAACAATGGCAAGCCACTGCGTATTGCTGTGCCTGAGCGAGTGAGTTACAAAGATTTTGTGTCTAAAGACAAGAGTCCTCCTGGGGTCAGAGGATATTGTATTGATATATTTGAAGCTGCTATAAACTTGTTGCCTTATCCTGTGCCACGCACATATATGTTGTATGGAGATGGCAAGAGGAATCCCCAGTACACCGATCTTGTTGGTCAAGTTGCTCTTAATCTCATGACTATTTTTTGGTTAAACACTGTGAGCACTCTAGGACGGCTGGTGCTGATTATATGGTTGTTTGTGGTGTTGATTATCAATTCGAGCTATACAGCTAGTTTGACATCGATCCTCACTGTACAACAGCTGACATCACGGATTGAGGGGATAGACAGCTTGATATCAAGTAATGATCCAATCGGAGTTCAAGATGGGTCATTTTCATGGAAATATTTGGTTAATGAGCTCAACATAGCAGAGTCGAGACTTGTTACCTTAAAAACCATGGAAGACTACATCAAAGCCCTTCAGGATGGACCAAAACGTGGTGGAGTAGCTGCCATTGTCGATGAGCTTCCTTACATTGAGCTGTTTATGTCTGCCACCAAGTGTGCATTCAGGACTGTGGGGCAGGAATTTACAAAAAGCGGATGGGGATTTGCATTCCAAAGGGATTCTCCTCTTGCTGTTGATTTGTCAACTGCCATTCTTCAACTCTCGGAGAACGGTGACCTCCAAAAGATCCATAATAAATGGCTTAGGAGTAGCGAGTGCTCTATGCAACTCAATGAAATGGATGCAGACCGACTATCCCTGACGAGCTTTTGGGGCCTGTTCCTTATATGCGGTATTGCATGCTTCATTGCTCTTGCTGTGTTCTTCTGTAGAATCTTATTGCAATACCGCAGATTTAGTCCAGAGACTGTGGAAGGGGATGTTGAGGAAATTGAACCTCCTCCCAGATCTAGTCGCCGCTCACTCCGGACAAATAGTTTCAAAGACTTGTTGGGTTTTGTAGATAAGAAAGAAGTGGAGATCAAGCATATGCTAAAGAGAAAGAGCAGCGATAACAAACAAGAAGCTAGCCCAAGCTCTGATGGCAAGTCCCATTCACCCTCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0004888 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0007154 GO:0007165 GO:0008066 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009507 GO:0009536 GO:0009605 GO:0009611 GO:0009612 GO:0009628 GO:0009719 GO:0009987 GO:0010033 GO:0010034 GO:0010243 GO:0015075 GO:0015085 GO:0015267 GO:0015276 GO:0015318 GO:0016020 GO:0019722 GO:0019932 GO:0022803 GO:0022834 GO:0022836 GO:0022838 GO:0022839 GO:0022857 GO:0022890 GO:0023052 GO:0030001 GO:0033554 GO:0034220 GO:0035556 GO:0038023 GO:0042221 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046677 GO:0046873 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055085 GO:0060089 GO:0065007 GO:0070417 GO:0070588 GO:0070838 GO:0070887 GO:0071214 GO:0071229 GO:0071230 GO:0071236 GO:0071260 GO:0071310 GO:0071311 GO:0071417 GO:0071495 GO:0071496 GO:0071944 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:0104004 GO:1901698 GO:1901699 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

816

Amino Acids

89.99

Weight (kDa)

6.11

Isoelectric Point (pI)

45.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peripla_BP_6	PF13458	19 - 315	3.8e-19	Periplasmic binding protein
ANF_receptor	PF01094	34 - 390	4.7e-89	Receptor family ligand binding region
Lig_chan	PF00060	528 - 728	1.2e-20	Ligand-gated ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001061)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G05200 AT1G05200 AT1G05200 AT1G05200 AT1G05200 AT1G05200 AT1G05200 AT2G32390 AT2G32390 AT2G32390 AT2G32390 AT2G32390 AT2G32390 AT2G32390
fragaria_vesca	FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291
malus_domestica	MD09G1069900.v1.1 MD09G1070100.v1.1 MD09G1107700.v1.1 MD09G1108100.v1.1 MD17G1096100.v1.1
prunus_persica	Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1
pyrus_communis	pycom09g03000 pycom09g03030 pycom09g03050 pycom09g03070 pycom15g20430 pycom17g09220
rosa_chinensis	RchiOBHm_Chr1g0324131 RchiOBHm_Chr2g0159571 RchiOBHm_Chr2g0159581
rosa_laevigata	RLG00000021167
rosa_multiflora	Rmu_co8397097.1_g000001 Rmu_sc0004966.1_g000017 Rmu_sc0020031.1_g000001
rosa_roxburghii	Rroxscaffold_2G00090390
rosa_rugosa	Rorug02G0476900 Rorug02G0477000 Rorug02G0477100 Rorug02G0477200
rosa_samantha	Rh2AG544200 Rh2BG557000 Rh2CG527200 Rh2DG566000
rosa_wichuraiana	Rw2G044990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	546
Acc36I	ACCTGC	1 cut(s)	1071
AccB7I	CCANNNNNTGG	1 cut(s)	1871
AccBSI	CCGCTC	1 cut(s)	2301
AccI	GTMKAC	1 cut(s)	1217
AccIII	TCCGGA	1 cut(s)	2307
AciI	CCGC	5 cut(s)	117, 1974, 2172, 2230, 2299
AclI	AACGTT	1 cut(s)	1057
AclWI	GGATC	4 cut(s)	690, 1669, 1733, 2062
AcoI	YGGCCR	1 cut(s)	96
AcsI	RAATTY	3 cut(s)	1129, 1201, 1961
AcuI	CTGAAG	2 cut(s)	1094, 1838
AdeI	CACNNNGTG	1 cut(s)	1935
AfaI	GTAC	7 cut(s)	318, 381, 504, 636, 756, 1529, 1686
AfiI	CCNNNNNNNGG	4 cut(s)	90, 653, 1343, 1871
AflII	CTTAAG	1 cut(s)	1076
AflIII	ACRYGT	1 cut(s)	637
AjnI	CCWGG	3 cut(s)	562, 1297, 1417
AjuI	GAANNNNNNNTTGG	2 cut(s)	1925, 1957
Alw21I	GWGCWC	5 cut(s)	64, 312, 1595, 1794, 2102
Alw26I	GTCTC	4 cut(s)	31, 1226, 1804, 2240
AlwI	GGATC	4 cut(s)	690, 1669, 1733, 2062
AlwNI	CAGNNNCTG	2 cut(s)	422, 2249
Ama87I	CYCGRG	3 cut(s)	152, 621, 1320
Aor13HI	TCCGGA	1 cut(s)	2307
AoxI	GGCC	6 cut(s)	38, 96, 259, 1051, 1295, 2155
ApeKI	GCWGC	7 cut(s)	226, 251, 551, 929, 1454, 1883, 2388
ApoI	RAATTY	3 cut(s)	1129, 1201, 1961
Asp700I	GAANNNNTTC	1 cut(s)	1250
AspLEI	GCGC	1 cut(s)	1016
AspS9I	GGNCC	3 cut(s)	260, 1862, 2155
AsuC2I	CCSGG	1 cut(s)	818
AsuHPI	GGTGA	3 cut(s)	521, 2066, 2430
AsuII	TTCGAA	1 cut(s)	1089
AsuNHI	GCTAGC	1 cut(s)	2408
AvaI	CYCGRG	3 cut(s)	152, 621, 1320
AvaII	GGWCC	1 cut(s)	1862
AxyI	CCTNAGG	1 cut(s)	271
BaeGI	GKGCMC	1 cut(s)	940
BanII	GRGCYC	4 cut(s)	64, 312, 571, 1794
BbsI	GAAGAC	1 cut(s)	1842
Bbv12I	GWGCWC	5 cut(s)	64, 312, 1595, 1794, 2102
BbvI	GCAGC	7 cut(s)	238, 238, 538, 941, 1441, 1870, 2400
BccI	CCATC	8 cut(s)	438, 1040, 1320, 1502, 1751, 1853, 1971, 2417
BceAI	ACGGC	1 cut(s)	1619
BciT130I	CCWGG	3 cut(s)	564, 1299, 1419
BciVI	GTATCC	1 cut(s)	814
BcnI	CCSGG	1 cut(s)	818
BcoDI	GTCTC	4 cut(s)	31, 1226, 1804, 2240
BfaI	CTAG	7 cut(s)	113, 506, 981, 1598, 1662, 2292, 2409
BfmI	CTRYAG	3 cut(s)	1064, 1308, 2209
BfrI	CTTAAG	1 cut(s)	1076
BfuAI	ACCTGC	1 cut(s)	1071
BfuI	GTATCC	1 cut(s)	814
BglII	AGATCT	1 cut(s)	2288
BisI	GCNGC	9 cut(s)	117, 227, 252, 552, 930, 1455, 1884, 2299, 2389
BlsI	GCNGC	9 cut(s)	118, 228, 253, 553, 931, 1456, 1885, 2300, 2390
BmcAI	AGTACT	1 cut(s)	504
Bme1390I	CCNGG	4 cut(s)	564, 818, 1299, 1419
Bme18I	GGWCC	1 cut(s)	1862
BmeT110I	CYCGRG	3 cut(s)	152, 621, 1320
BmgT120I	GGNCC	3 cut(s)	260, 1862, 2155
BmiI	GGNNCC	2 cut(s)	568, 2156
BmrFI	CCNGG	4 cut(s)	564, 818, 1299, 1419
BmrI	ACTGGG	1 cut(s)	1519
BmsI	GCATC	4 cut(s)	502, 854, 1037, 2113
BmtI	GCTAGC	1 cut(s)	2412
BmuI	ACTGGG	1 cut(s)	1519
BoxI	GACNNNNGTC	1 cut(s)	1410
BpiI	GAAGAC	1 cut(s)	1842
BplI	GAGNNNNNCTC	2 cut(s)	594, 626
BpmI	CTGGAG	1 cut(s)	585
Bpu10I	CCTNAGC	3 cut(s)	517, 1371, 2084
Bpu14I	TTCGAA	1 cut(s)	1089
BpuMI	CCSGG	1 cut(s)	818
Bsa29I	ATCGAT	1 cut(s)	1673
BsaBI	GATNNNNATC	1 cut(s)	825
BsaJI	CCNNGG	5 cut(s)	678, 816, 1319, 1418, 1830
BsaWI	WCCGGW	1 cut(s)	2307
BsaXI	ACNNNNNCTCC	4 cut(s)	954, 984, 1255, 1285
Bsc4I	CCNNNNNNNGG	4 cut(s)	90, 653, 1343, 1871
Bse1I	ACTGG	4 cut(s)	16, 699, 1177, 1525
Bse21I	CCTNAGG	1 cut(s)	271
Bse3DI	GCAATG	1 cut(s)	2187
Bse8I	GATNNNNATC	1 cut(s)	825
BseAI	TCCGGA	1 cut(s)	2307
BseBI	CCWGG	3 cut(s)	564, 1299, 1419
BseCI	ATCGAT	1 cut(s)	1673
BseDI	CCNNGG	5 cut(s)	678, 816, 1319, 1418, 1830
BseGI	GGATG	7 cut(s)	449, 1138, 1331, 1864, 1982, 2128, 2266
BseJI	GATNNNNATC	1 cut(s)	825
BseLI	CCNNNNNNNGG	4 cut(s)	90, 653, 1343, 1871
BseMI	GCAATG	1 cut(s)	2187
BseMII	CTCAG	3 cut(s)	20, 285, 1362
BseNI	ACTGG	4 cut(s)	16, 699, 1177, 1525
BseRI	GAGGAG	2 cut(s)	1995, 2271
BseSI	GKGCMC	1 cut(s)	940
BseXI	GCAGC	7 cut(s)	238, 238, 538, 941, 1441, 1870, 2400
BsgI	GTGCAG	1 cut(s)	1004
BshFI	GGCC	6 cut(s)	40, 98, 261, 1053, 1297, 2157
BshVI	ATCGAT	1 cut(s)	1673
BsiHKAI	GWGCWC	5 cut(s)	64, 312, 1595, 1794, 2102
BsiHKCI	CYCGRG	3 cut(s)	152, 621, 1320
BsiSI	CCGG	2 cut(s)	818, 2308
BslFI	GGGAC	3 cut(s)	172, 979, 2416
BslI	CCNNNNNNNGG	4 cut(s)	90, 653, 1343, 1871
BsmAI	GTCTC	4 cut(s)	31, 1226, 1804, 2240
BsmBI	CGTCTC	1 cut(s)	31
BsmFI	GGGAC	3 cut(s)	172, 979, 2416
BsmI	GAATGC	2 cut(s)	1940, 1988
BsnI	GGCC	6 cut(s)	40, 98, 261, 1053, 1297, 2157
BsoBI	CYCGRG	3 cut(s)	152, 621, 1320
Bsp119I	TTCGAA	1 cut(s)	1089
Bsp1286I	GDGCHC	7 cut(s)	64, 312, 571, 940, 1595, 1794, 2102
Bsp13I	TCCGGA	1 cut(s)	2307
Bsp1407I	TGTACA	2 cut(s)	634, 1684
Bsp143I	GATC	9 cut(s)	682, 826, 1117, 1534, 1674, 1738, 2067, 2288, 2361
Bsp19I	CCATGG	1 cut(s)	1830
BspACI	CCGC	5 cut(s)	117, 1974, 2172, 2230, 2299
BspANI	GGCC	6 cut(s)	40, 98, 261, 1053, 1297, 2157
BspCNI	CTCAG	3 cut(s)	19, 284, 1363
BspDI	ATCGAT	1 cut(s)	1673
BspEI	TCCGGA	1 cut(s)	2307
BspHI	TCATGA	1 cut(s)	1563
BspLI	GGNNCC	2 cut(s)	568, 2156
BspMAI	CTGCAG	1 cut(s)	1068
BspMI	ACCTGC	1 cut(s)	1071
BspOI	GCTAGC	1 cut(s)	2412
BspPI	GGATC	4 cut(s)	690, 1669, 1733, 2062
BspQI	GCTCTTC	1 cut(s)	807
BspT104I	TTCGAA	1 cut(s)	1089
BspTI	CTTAAG	1 cut(s)	1076
BsrBI	CCGCTC	1 cut(s)	2301
BsrDI	GCAATG	1 cut(s)	2187
BsrGI	TGTACA	2 cut(s)	634, 1684
BsrI	ACTGG	4 cut(s)	16, 699, 1177, 1525
BssECI	CCNNGG	5 cut(s)	678, 816, 1319, 1418, 1830
BssMI	GATC	9 cut(s)	682, 826, 1117, 1534, 1674, 1738, 2067, 2288, 2361
BssT1I	CCWWGG	2 cut(s)	678, 1830
Bst2UI	CCWGG	3 cut(s)	564, 1299, 1419
Bst4CI	ACNGT	9 cut(s)	25, 214, 267, 970, 1588, 1684, 1951, 2054, 2251
Bst6I	CTCTTC	1 cut(s)	807
BstAFI	CTTAAG	1 cut(s)	1076
BstAPI	GCANNNNNTGC	1 cut(s)	935
BstAUI	TGTACA	2 cut(s)	634, 1684
BstBI	TTCGAA	1 cut(s)	1089
BstC8I	GCNNGC	3 cut(s)	1349, 2182, 2410
BstDEI	CTNAG	7 cut(s)	6, 33, 271, 517, 1024, 1371, 2084
BstDSI	CCRYGG	1 cut(s)	1830
BstEII	GGTNACC	1 cut(s)	2054
BstF5I	GGATG	7 cut(s)	449, 1138, 1331, 1864, 1982, 2128, 2266
BstHHI	GCGC	1 cut(s)	1016
BstKTI	GATC	9 cut(s)	685, 829, 1120, 1537, 1677, 1741, 2070, 2291, 2364
BstMAI	GTCTC	4 cut(s)	31, 1226, 1804, 2240
BstMBI	GATC	9 cut(s)	682, 826, 1117, 1534, 1674, 1738, 2067, 2288, 2361
BstMWI	GCNNNNNNNGC	7 cut(s)	104, 113, 548, 905, 935, 2154, 2424
BstNI	CCWGG	3 cut(s)	564, 1299, 1419
BstNSI	RCATGY	2 cut(s)	641, 2184
BstPAI	GACNNNNGTC	1 cut(s)	1410
BstPI	GGTNACC	1 cut(s)	2054
BstSCI	CCNGG	4 cut(s)	562, 816, 1297, 1417
BstSFI	CTRYAG	3 cut(s)	1064, 1308, 2209
BstSLI	GKGCMC	1 cut(s)	940
BstV1I	GCAGC	7 cut(s)	238, 238, 538, 941, 1441, 1870, 2400
BstV2I	GAAGAC	1 cut(s)	1842
BstX2I	RGATCY	3 cut(s)	682, 2067, 2288
BstYI	RGATCY	3 cut(s)	682, 2067, 2288
Bsu15I	ATCGAT	1 cut(s)	1673
Bsu36I	CCTNAGG	1 cut(s)	271
BsuI	GTATCC	1 cut(s)	814
BsuRI	GGCC	6 cut(s)	40, 98, 261, 1053, 1297, 2157
BsuTUI	ATCGAT	1 cut(s)	1673
BtgI	CCRYGG	1 cut(s)	1830
BtsCI	GGATG	7 cut(s)	449, 1138, 1331, 1864, 1982, 2128, 2266
BtsI	GCAGTG	3 cut(s)	202, 1016, 1352
BtsIMutI	CAGTG	6 cut(s)	202, 706, 1016, 1352, 1584, 1680
BveI	ACCTGC	1 cut(s)	1071
Cac8I	GCNNGC	3 cut(s)	1349, 2182, 2410
CaiI	CAGNNNCTG	2 cut(s)	422, 2249
CciI	TCATGA	1 cut(s)	1563
CfoI	GCGC	1 cut(s)	1016
Cfr13I	GGNCC	3 cut(s)	260, 1862, 2155
ClaI	ATCGAT	1 cut(s)	1673
Csp6I	GTAC	7 cut(s)	317, 380, 503, 635, 755, 1528, 1685
CviQI	GTAC	7 cut(s)	317, 380, 503, 635, 755, 1528, 1685
DdeI	CTNAG	7 cut(s)	6, 33, 271, 517, 1024, 1371, 2084
DpnI	GATC	9 cut(s)	684, 828, 1119, 1536, 1676, 1740, 2069, 2290, 2363
DpnII	GATC	9 cut(s)	682, 826, 1117, 1534, 1674, 1738, 2067, 2288, 2361
DraIII	CACNNNGTG	1 cut(s)	1935
EaeI	YGGCCR	1 cut(s)	96
Eam1104I	CTCTTC	1 cut(s)	807
EarI	CTCTTC	1 cut(s)	807
Ecl136II	GAGCTC	3 cut(s)	62, 310, 1792
Eco130I	CCWWGG	2 cut(s)	678, 1830
Eco24I	GRGCYC	4 cut(s)	64, 312, 571, 1794
Eco32I	GATATC	2 cut(s)	583, 1728
Eco47I	GGWCC	1 cut(s)	1862
Eco53kI	GAGCTC	3 cut(s)	62, 310, 1792
Eco57I	CTGAAG	2 cut(s)	1094, 1838
Eco81I	CCTNAGG	1 cut(s)	271
Eco88I	CYCGRG	3 cut(s)	152, 621, 1320
Eco91I	GGTNACC	1 cut(s)	2054
EcoICRI	GAGCTC	3 cut(s)	62, 310, 1792
EcoO109I	RGGNCCY	1 cut(s)	2155
EcoO65I	GGTNACC	1 cut(s)	2054
EcoRII	CCWGG	3 cut(s)	562, 1297, 1417
EcoRV	GATATC	2 cut(s)	583, 1728
EcoT14I	CCWWGG	2 cut(s)	678, 1830
EcoT22I	ATGCAT	1 cut(s)	910
EcoT38I	GRGCYC	4 cut(s)	64, 312, 571, 1794
ErhI	CCWWGG	2 cut(s)	678, 1830
Esp3I	CGTCTC	1 cut(s)	31
FalI	AAGNNNNNCTT	6 cut(s)	539, 571, 1070, 1102, 1539, 1571
FaqI	GGGAC	3 cut(s)	172, 979, 2416
FauNDI	CATATG	2 cut(s)	910, 2370
FblI	GTMKAC	1 cut(s)	1217
Fnu4HI	GCNGC	9 cut(s)	117, 227, 252, 552, 930, 1455, 1884, 2299, 2389
FokI	GGATG	7 cut(s)	456, 1125, 1338, 1871, 1989, 2135, 2273
FriOI	GRGCYC	4 cut(s)	64, 312, 571, 1794
Fsp4HI	GCNGC	9 cut(s)	117, 227, 252, 552, 930, 1455, 1884, 2299, 2389
FspBI	CTAG	7 cut(s)	113, 506, 981, 1598, 1662, 2292, 2409
GlaI	GCGC	1 cut(s)	1015
GluI	GCNGC	9 cut(s)	117, 227, 252, 552, 930, 1455, 1884, 2299, 2389
GsuI	CTGGAG	1 cut(s)	585
HaeIII	GGCC	6 cut(s)	40, 98, 261, 1053, 1297, 2157
HapII	CCGG	2 cut(s)	818, 2308
HhaI	GCGC	1 cut(s)	1016
Hin6I	GCGC	1 cut(s)	1014
HinP1I	GCGC	1 cut(s)	1014
HincII	GTYRAC	2 cut(s)	1218, 2025
HindII	GTYRAC	2 cut(s)	1218, 2025
HindIII	AAGCTT	1 cut(s)	219
HpaII	CCGG	2 cut(s)	818, 2308
HphI	GGTGA	3 cut(s)	521, 2066, 2430
Hpy166II	GTNNAC	4 cut(s)	607, 1218, 1530, 2025
Hpy8I	GTNNAC	4 cut(s)	607, 1218, 1530, 2025
HpyAV	CCTTC	7 cut(s)	84, 565, 866, 953, 1085, 1862, 2249
HpyCH4III	ACNGT	9 cut(s)	25, 214, 267, 970, 1588, 1684, 1951, 2054, 2251
HpyCH4IV	ACGT	3 cut(s)	297, 1057, 1870
HpyF10VI	GCNNNNNNNGC	7 cut(s)	104, 113, 548, 905, 935, 2154, 2424
HpyF3I	CTNAG	7 cut(s)	6, 33, 271, 517, 1024, 1371, 2084
HpySE526I	ACGT	3 cut(s)	297, 1057, 1870
HspAI	GCGC	1 cut(s)	1014
Kpn2I	TCCGGA	1 cut(s)	2307
Kzo9I	GATC	9 cut(s)	682, 826, 1117, 1534, 1674, 1738, 2067, 2288, 2361
LguI	GCTCTTC	1 cut(s)	807
LmnI	GCTCC	5 cut(s)	59, 315, 335, 566, 855
Lsp1109I	GCAGC	7 cut(s)	238, 238, 538, 941, 1441, 1870, 2400
LweI	GCATC	4 cut(s)	502, 854, 1037, 2113
MaeI	CTAG	7 cut(s)	113, 506, 981, 1598, 1662, 2292, 2409
MaeII	ACGT	3 cut(s)	297, 1057, 1870
MaeIII	GTNAC	6 cut(s)	395, 983, 1058, 1382, 1816, 2054
MalI	GATC	9 cut(s)	684, 828, 1119, 1536, 1676, 1740, 2069, 2290, 2363
MbiI	CCGCTC	1 cut(s)	2301
MboI	GATC	9 cut(s)	682, 826, 1117, 1534, 1674, 1738, 2067, 2288, 2361
MboII	GAAGA	6 cut(s)	137, 794, 1197, 1847, 2027, 2197
MfeI	CAATTG	2 cut(s)	120, 255
MflI	RGATCY	3 cut(s)	682, 2067, 2288
MhlI	GDGCHC	7 cut(s)	64, 312, 571, 940, 1595, 1794, 2102
MlyI	GAGTC	4 cut(s)	644, 908, 1420, 1814
Mph1103I	ATGCAT	1 cut(s)	910
MroI	TCCGGA	1 cut(s)	2307
MroXI	GAANNNNTTC	1 cut(s)	1250
MseI	TTAA	9 cut(s)	132, 303, 575, 1077, 1133, 1557, 1580, 1785, 1823
MslI	CAYNNNNRTG	3 cut(s)	850, 1493, 1768
MspA1I	CMGCKG	1 cut(s)	1693
MspCI	CTTAAG	1 cut(s)	1076
MspI	CCGG	2 cut(s)	818, 2308
MspR9I	CCNGG	4 cut(s)	564, 818, 1299, 1419
MunI	CAATTG	2 cut(s)	120, 255
Mva1269I	GAATGC	2 cut(s)	1940, 1988
MvaI	CCWGG	3 cut(s)	564, 1299, 1419
MwoI	GCNNNNNNNGC	7 cut(s)	104, 113, 548, 905, 935, 2154, 2424
NciI	CCSGG	1 cut(s)	818
NcoI	CCATGG	1 cut(s)	1830
NdeI	CATATG	2 cut(s)	910, 2370
NdeII	GATC	9 cut(s)	682, 826, 1117, 1534, 1674, 1738, 2067, 2288, 2361
NheI	GCTAGC	1 cut(s)	2408
NlaIV	GGNNCC	2 cut(s)	568, 2156
NmeAIII	GCCGAG	1 cut(s)	66
NmuCI	GTSAC	3 cut(s)	395, 983, 2054
NsiI	ATGCAT	1 cut(s)	910
NspI	RCATGY	2 cut(s)	641, 2184
NspV	TTCGAA	1 cut(s)	1089
PaeI	GCATGC	1 cut(s)	2184
PaeR7I	CTCGAG	1 cut(s)	152
PagI	TCATGA	1 cut(s)	1563
PciI	ACATGT	1 cut(s)	637
PciSI	GCTCTTC	1 cut(s)	807
PctI	GAATGC	2 cut(s)	1940, 1988
PdmI	GAANNNNTTC	1 cut(s)	1250
PfeI	GAWTC	7 cut(s)	140, 617, 746, 778, 1519, 2000, 2214
PflMI	CCANNNNNTGG	1 cut(s)	1871
PfoI	TCCNGGA	1 cut(s)	562
PkrI	GCNGC	9 cut(s)	118, 228, 253, 553, 931, 1456, 1885, 2300, 2390
PleI	GAGTC	4 cut(s)	644, 908, 1419, 1813
PpsI	GAGTC	4 cut(s)	644, 908, 1419, 1813
PscI	ACATGT	1 cut(s)	637
PshAI	GACNNNNGTC	1 cut(s)	1410
PsiI	TTATAA	1 cut(s)	546
Psp124BI	GAGCTC	3 cut(s)	64, 312, 1794
Psp1406I	AACGTT	1 cut(s)	1057
Psp6I	CCWGG	3 cut(s)	562, 1297, 1417
PspEI	GGTNACC	1 cut(s)	2054
PspGI	CCWGG	3 cut(s)	562, 1297, 1417
PspN4I	GGNNCC	2 cut(s)	568, 2156
PspPI	GGNCC	3 cut(s)	260, 1862, 2155
PstI	CTGCAG	1 cut(s)	1068
PstNI	CAGNNNCTG	2 cut(s)	422, 2249
PsuI	RGATCY	3 cut(s)	682, 2067, 2288
PvuII	CAGCTG	1 cut(s)	1693
RsaI	GTAC	7 cut(s)	318, 381, 504, 636, 756, 1529, 1686
RsaNI	GTAC	7 cut(s)	317, 380, 503, 635, 755, 1528, 1685
RseI	CAYNNNNRTG	3 cut(s)	850, 1493, 1768
SacI	GAGCTC	3 cut(s)	64, 312, 1794
SalI	GTCGAC	1 cut(s)	1216
SapI	GCTCTTC	1 cut(s)	807
SaqAI	TTAA	9 cut(s)	132, 303, 575, 1077, 1133, 1557, 1580, 1785, 1823
SatI	GCNGC	9 cut(s)	117, 227, 252, 552, 930, 1455, 1884, 2299, 2389
Sau3AI	GATC	9 cut(s)	682, 826, 1117, 1534, 1674, 1738, 2067, 2288, 2361
Sau96I	GGNCC	3 cut(s)	260, 1862, 2155
ScaI	AGTACT	1 cut(s)	504
SchI	GAGTC	4 cut(s)	644, 908, 1420, 1814
ScrFI	CCNGG	4 cut(s)	564, 818, 1299, 1419
SduI	GDGCHC	7 cut(s)	64, 312, 571, 940, 1595, 1794, 2102
SfaNI	GCATC	4 cut(s)	502, 854, 1037, 2113
SfcI	CTRYAG	3 cut(s)	1064, 1308, 2209
Sfr274I	CTCGAG	1 cut(s)	152
SfuI	TTCGAA	1 cut(s)	1089
SinI	GGWCC	1 cut(s)	1862
SlaI	CTCGAG	1 cut(s)	152
SmiMI	CAYNNNNRTG	3 cut(s)	850, 1493, 1768
SmlI	CTYRAG	2 cut(s)	152, 1076
SmoI	CTYRAG	2 cut(s)	152, 1076
SphI	GCATGC	1 cut(s)	2184
SsiI	CCGC	5 cut(s)	117, 1974, 2172, 2230, 2299
SspI	AATATT	3 cut(s)	55, 1041, 1778
SspMI	CTAG	7 cut(s)	113, 506, 981, 1598, 1662, 2292, 2409
SstI	GAGCTC	3 cut(s)	64, 312, 1794
StyD4I	CCNGG	4 cut(s)	562, 816, 1297, 1417
StyI	CCWWGG	2 cut(s)	678, 1830
TaaI	ACNGT	9 cut(s)	25, 214, 267, 970, 1588, 1684, 1951, 2054, 2251
TaiI	ACGT	3 cut(s)	300, 1060, 1873
TaqI	TCGA	7 cut(s)	153, 1089, 1217, 1649, 1673, 1808, 1893
TaqII	GACCGA	1 cut(s)	2145
TatI	WGTACW	4 cut(s)	502, 634, 1527, 1684
TauI	GCSGC	2 cut(s)	119, 2301
TfiI	GAWTC	7 cut(s)	140, 617, 746, 778, 1519, 2000, 2214
Tru1I	TTAA	9 cut(s)	132, 303, 575, 1077, 1133, 1557, 1580, 1785, 1823
Tru9I	TTAA	9 cut(s)	132, 303, 575, 1077, 1133, 1557, 1580, 1785, 1823
TscAI	CASTG	6 cut(s)	202, 706, 1023, 1359, 1591, 1687
TseFI	GTSAC	3 cut(s)	395, 983, 2054
TseI	GCWGC	7 cut(s)	226, 251, 551, 929, 1454, 1883, 2388
Tsp45I	GTSAC	3 cut(s)	395, 983, 2054
TspDTI	ATGAA	9 cut(s)	738, 791, 885, 972, 1097, 1127, 1758, 2130, 2176
TspGWI	ACGGA	1 cut(s)	1717
TspRI	CASTG	6 cut(s)	202, 706, 1023, 1359, 1591, 1687
Van91I	CCANNNNNTGG	1 cut(s)	1871
Vha464I	CTTAAG	1 cut(s)	1076
VpaK11BI	GGWCC	1 cut(s)	1862
XapI	RAATTY	3 cut(s)	1129, 1201, 1961
XceI	RCATGY	2 cut(s)	641, 2184
XhoI	CTCGAG	1 cut(s)	152
XmiI	GTMKAC	1 cut(s)	1217
XmnI	GAANNNNTTC	1 cut(s)	1250
XspI	CTAG	7 cut(s)	113, 506, 981, 1598, 1662, 2292, 2409
ZrmI	AGTACT	1 cut(s)	504
Zsp2I	ATGCAT	1 cut(s)	910

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.