Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0001061

Physical Location & Seq

Genomic Coordinates Reverse (-)

4822947 .. 4829801

Gene Structure 6855 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1070100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2964 bp

ATGGAGGTATTGCTGATGAATAGGCCTACTGACCATGTATGCAAGACAACACTGTTTGCGTTGATCTTGTGTATGTGGGTGTCCATGCAAGTGATGGCTGGAACAGAAAATGCCACGCATTTGTCTGCAAGGCCAAGTTCTCTGAATATTGGAGCTCTGTTTACTTTCAATTCGGTTATTGGGAGGGCAGCCAAGCCGGCAATTTTAGCTGCAATTGATGATGTCAATTCTGATCAAAGCATTCTTCGCGGGACAAAATTGAACATTATTCTCCATGACACGAATTGCAGTGAATTTCTTGGAACTGTTGAAGCTATGGAGCTGATTAACAACGACGTGGTTGCTGCAGTCGGCCCACAATCCTCTGGAATAGCTCATGTCATATCCCATGTTGTTAATGAGCTCCATGTACCACTTCTATCATTTGGAGCAACAGACCCCTCCCTTGCTGCTCTCAAATACCCATATTTTGTCCGAACCACACAGAGTGACTATTTCCAAATGTATGCAGTTGCTGATTTAGTTGAGTATTTTGGATGGAGAGAAGTAATAGCAATCTTTGTAGATGATGATTATGGCCGAAATGGGATTTCTATACTAGCCGATGCCTTGGCAGTGAAGCGTTCTAAGATCTCATACAAGGCTGCCTTCTCTCCTGGAGCCCTCAAAAGCGATATCAATGAATTGTTGATGGGAGTGAACCTCATGGAATCTCGGGTTTATATTGTACATGTTCATCCCGACTCCGGTTTAACTATTTTTTCCATTGCCAAGGCACTTGGAATGATGACCAGTGGCTATGTCTGGATTGCGACGGATTGGCTTCCTTCCTATTTAGATTCACTAAATCCCCCTGGCCCTGACACAACGAATCTCTTACAAGGGGTTGTTGCTCTCCGTCATCATACCCCAGATACTGATCTGAAAAAGCGCTTTATGTCTAGATGGAGCAAATTGAAACATGAAGGGACTCCAAGCTTCAATTCTTATGCGCTTTATGCATACGACTCTATTTGGCTAGCAGCCCGTGCTCTTGATGTTTTCTTCAACGAAGGTGGGAATGTATCTTTCTCTAATTACCCAAGGTTGAAAGACACAAATCGAAGCACACTGCATTTAACATCACTCCGCATTTTTTATGGAGGCCAAAAATATCTACAGACGATTCTTAAGATGAACTTCACAGGTATAAGCGGTCAGATTGAGTTTGATCAAGATAAATATCTAGTTCGTCCAGCGTATGACATTCTGAATATTGGAGGAACTGGTTCTCGTAGAGTTGGCTATTGGTCAAATTCTACTGGTCTCTCGGTCATTGCTCCAGAGATATTATATAAGAAGCCATTCAATAGGAATACGACTGCTCAACTTTATAGTGTAATATGGCCTGGTGAAGTTACAGCTACACCTCGGGGATGGGTATTTCCCAACAACGGCAGGCCACTGCGAATTGGAGTGCCTTATCGAGTAAGTTACAAAGATTTTGTGGCTAAAGACAAGAGCCCTGCAGGGGTCAGAGGATACTGTATTGATGTCTTTGAAGCTGCTGTAAACTTGTTGCCGTATGCTGTGCCACGTACGTATATGTTGTATGGAGATGGTAAGAGGAATCCTGAGTACAGCAGTCTTGTAGCCCAGGTTGCGCAAAATAAATTTGATGCAGCTGTTGGAGATGTTACAATTACTAATAATAGGACAAGAATAGTTGATTTTACGCAGCCTTACATGGAATCGGGACTTGTTGTAGTTGTTCCTGTCAAAAAGGCAAAATCAAACCCTTGGGTTTTCCTCAAGCCGTTTACTTACCAGATGTGGTTGGTCACCGGTGCCTTCTTCCTTTTTGTGGGAGCTGTTGTTTGGATTCTTGAGCACCGGTTCAATCATGAGTTCCGTGGTCCACCAAGCCAACAGCTCATAACCATTTTTTGGTTAGTATGTCTTACATCATTATGGCCGTTGATAAGTGCCTATACAATATGTACTTCACGCTTGATCTTCCTGTATCTCGTGAATGCAACTCGTAATATTAACTACTTATATCCTTTGCGACTTGGACTTGGTGCAGGAGAGAACACTGTGAGCACCCTGGGACGGCTGGTGGTGATTATATGGTTATTTGTAGTGTTAATTATCAATTCTAGCTACACTGCTAGTTTGACTTCAATTCTTACAGTGCAACAGCTGACGTCACGGATTGAAGGGATTGATAGCTTGATATCAAGTAATGATCGAATCGGAGTTCAAGACGGGTCATTTGCTTGGAGGTATTTGGTTGATGAGATGAACATAGCGGAATCTAGACTTGTTAAGTTGAAAGACATGGAAGCCTATATTAAAGCCCTTACGGATGGACCAAGACGTGGTGGGGTAGCTGCCATCGTTGATGAGCTTCCTTACATTGAGTTGTTCATGTCCAGCACCAAATGTGCATTCAGGACTGTAGGGCTGGAGTTTACCAAAAGCGGATGGGGATTCGCCTTCCAAAAGGATTCTCCTCTTGCTGTTGACTTGTCGACAGCGATTCTTCAACTTTCAGAGAACGGTGATCTACAAAAGATCCATGATAAGTGGTTTAAGCATAACGAGTGCCCTACTCAACTCAATGACGTTGATGCAGACCTGCAGCTATCTCTAACAAGCTTTTGGGGCCTGTTTCTTATCTGCGGTATTGCATGCTTCCTTGCTCTTGCGGTGTTCTTCTGTAGAATCCTTTGTCAATACCGCAGATTTACCCCAGAGCCTGTGGAAGCGGACGCTGAGGAGATTGGACCTACCAACACGAGATCTAGACGCTCACTTGGGTCAACCAGTTTCAAGGGTCTTATGGTCTTTGTAGATAAGAAAGAAGCAGAGATAAAGCACATGCTGAAGCGAAAGACTAGTGATAGTAAGCACGAAGCTAGCCCGAGCGCTGATGGGCAGCTGCATTCCCTTCGTGAGGAAGATTTGCCTTCGGCGGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0004888 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0007154 GO:0007165 GO:0008066 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009507 GO:0009536 GO:0009605 GO:0009611 GO:0009612 GO:0009628 GO:0009719 GO:0009987 GO:0010033 GO:0010034 GO:0010243 GO:0015075 GO:0015085 GO:0015267 GO:0015276 GO:0015318 GO:0016020 GO:0019722 GO:0019932 GO:0022803 GO:0022834 GO:0022836 GO:0022838 GO:0022839 GO:0022857 GO:0022890 GO:0023052 GO:0030001 GO:0033554 GO:0034220 GO:0035556 GO:0038023 GO:0042221 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046677 GO:0046873 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055085 GO:0060089 GO:0065007 GO:0070417 GO:0070588 GO:0070838 GO:0070887 GO:0071214 GO:0071229 GO:0071230 GO:0071236 GO:0071260 GO:0071310 GO:0071311 GO:0071417 GO:0071495 GO:0071496 GO:0071944 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:0104004 GO:1901698 GO:1901699 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

988

Amino Acids

110.1

Weight (kDa)

8.07

Isoelectric Point (pI)

38.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peripla_BP_6	PF13458	50 - 345	1.8e-18	Periplasmic binding protein
ANF_receptor	PF01094	64 - 421	4.8e-89	Receptor family ligand binding region
Lig_chan-Glu_bd	PF10613	500 - 586	5.7e-12	Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3	PF00497	539 - 859	6.7e-14	Bacterial extracellular solute-binding proteins, family 3
Lig_chan	PF00060	602 - 646	5.5e-06	Ligand-gated ion channel
Lig_chan	PF00060	686 - 892	1.4e-22	Ligand-gated ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0001061)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G05200 AT1G05200 AT1G05200 AT1G05200 AT1G05200 AT1G05200 AT1G05200 AT2G32390 AT2G32390 AT2G32390 AT2G32390 AT2G32390 AT2G32390 AT2G32390
fragaria_vesca	FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291
malus_domestica	MD09G1069900.v1.1 MD09G1070100.v1.1 MD09G1107700.v1.1 MD09G1108100.v1.1 MD17G1096100.v1.1
prunus_persica	Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1
pyrus_communis	pycom09g03000 pycom09g03030 pycom09g03050 pycom09g03070 pycom15g20430 pycom17g09220
rosa_chinensis	RchiOBHm_Chr1g0324131 RchiOBHm_Chr2g0159571 RchiOBHm_Chr2g0159581
rosa_laevigata	RLG00000021167
rosa_multiflora	Rmu_co8397097.1_g000001 Rmu_sc0004966.1_g000017 Rmu_sc0020031.1_g000001
rosa_roxburghii	Rroxscaffold_2G00090390
rosa_rugosa	Rorug02G0476900 Rorug02G0477000 Rorug02G0477100 Rorug02G0477200
rosa_samantha	Rh2AG544200 Rh2BG557000 Rh2CG527200 Rh2DG566000
rosa_wichuraiana	Rw2G044990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	2189
Acc16I	TGCGCA	1 cut(s)	1644
Acc36I	ACCTGC	1 cut(s)	2628
AccB1I	GGYRCC	1 cut(s)	1826
AccB7I	CCANNNNNTGG	2 cut(s)	1926, 2360
AccI	GTMKAC	1 cut(s)	2513
AccII	CGCG	1 cut(s)	249
AclWI	GGATC	1 cut(s)	2551
AcoI	YGGCCR	2 cut(s)	577, 1952
AcsI	RAATTY	3 cut(s)	293, 1294, 1652
AcuI	CTGAAG	1 cut(s)	2888
AcyI	GRCGYC	1 cut(s)	2186
AdeI	CACNNNGTG	1 cut(s)	488
AfaI	GTAC	5 cut(s)	411, 729, 1579, 1619, 1981
AfeI	AGCGCT	2 cut(s)	932, 2911
AfiI	CCNNNNNNNGG	9 cut(s)	746, 1433, 1510, 1843, 1926, 2091, 2360, 2938, 2957
AflII	CTTAAG	1 cut(s)	1169
AflIII	ACRYGT	1 cut(s)	730
AgeI	ACCGGT	2 cut(s)	1823, 1872
AhlI	ACTAGT	1 cut(s)	2879
AjiI	CACGTC	2 cut(s)	337, 2360
AjnI	CCWGG	5 cut(s)	655, 853, 1387, 1635, 2085
AjuI	GAANNNNNNNTTGG	4 cut(s)	967, 999, 2414, 2446
Alw21I	GWGCWC	5 cut(s)	157, 405, 1033, 1872, 2084
Alw26I	GTCTC	1 cut(s)	1310
AlwI	GGATC	1 cut(s)	2551
AlwNI	CAGNNNCTG	3 cut(s)	515, 917, 2741
Ama87I	CYCGRG	3 cut(s)	714, 1410, 2905
Aor51HI	AGCGCT	2 cut(s)	932, 2911
ApoI	RAATTY	3 cut(s)	293, 1294, 1652
ArsI	GACNNNNNNTTYG	2 cut(s)	2237, 2269
AsiGI	ACCGGT	2 cut(s)	1823, 1872
Asp700I	GAANNNNTTC	2 cut(s)	1267, 1343
AspLEI	GCGC	4 cut(s)	933, 994, 1645, 2912
AspS9I	GGNCC	6 cut(s)	353, 857, 1895, 2351, 2647, 2768
AsuHPI	GGTGA	4 cut(s)	1403, 1813, 2113, 2555
AsuNHI	GCTAGC	2 cut(s)	1018, 2900
AvaI	CYCGRG	3 cut(s)	714, 1410, 2905
AvaII	GGWCC	3 cut(s)	1895, 2351, 2768
BaeGI	GKGCMC	1 cut(s)	2591
BanI	GGYRCC	1 cut(s)	1826
BanII	GRGCYC	4 cut(s)	157, 405, 664, 1505
BauI	CACGAG	2 cut(s)	2006, 2779
Bbv12I	GWGCWC	5 cut(s)	157, 405, 1033, 1872, 2084
BbvCI	CCTCAGC	1 cut(s)	2757
BceAI	ACGGC	5 cut(s)	1450, 1546, 1780, 1939, 2108
BcgI	CGANNNNNNTGC	4 cut(s)	323, 357, 2915, 2949
BciT130I	CCWGG	5 cut(s)	657, 855, 1389, 1637, 2087
BciVI	GTATCC	1 cut(s)	1514
BclI	TGATCA	2 cut(s)	232, 1210
BcoDI	GTCTC	1 cut(s)	1310
BcuI	ACTAGT	1 cut(s)	2879
BfmI	CTRYAG	6 cut(s)	345, 1157, 1506, 2439, 2621, 2701
BfoI	RGCGCY	2 cut(s)	934, 2913
BfrI	CTTAAG	1 cut(s)	1169
BfuAI	ACCTGC	1 cut(s)	2628
BfuI	GTATCC	1 cut(s)	1514
BglI	GCCNNNNNGGC	1 cut(s)	197
BglII	AGATCT	2 cut(s)	630, 2783
Bme1390I	CCNGG	5 cut(s)	657, 855, 1389, 1637, 2087
Bme18I	GGWCC	3 cut(s)	1895, 2351, 2768
BmeT110I	CYCGRG	3 cut(s)	714, 1410, 2905
BmgBI	CACGTC	2 cut(s)	337, 2360
BmgT120I	GGNCC	6 cut(s)	353, 857, 1895, 2351, 2647, 2768
BmiI	GGNNCC	3 cut(s)	661, 1828, 2648
BmrFI	CCNGG	5 cut(s)	657, 855, 1389, 1637, 2087
BmsI	GCATC	3 cut(s)	595, 1648, 2602
BmtI	GCTAGC	2 cut(s)	1022, 2904
BplI	GAGNNNNNCTC	2 cut(s)	687, 719
BpmI	CTGGAG	3 cut(s)	678, 1305, 2468
Bpu10I	CCTNAGC	1 cut(s)	2757
BpuEI	CTTGAG	2 cut(s)	1775, 1886
BsaAI	YACGTR	2 cut(s)	1577, 1581
BsaBI	GATNNNNATC	2 cut(s)	918, 1221
BsaHI	GRCGYC	1 cut(s)	2186
BsaI	GGTCTC	1 cut(s)	1310
BsaWI	WCCGGW	3 cut(s)	746, 1823, 1872
BsaXI	ACNNNNNCTCC	2 cut(s)	1839, 1869
Bsc4I	CCNNNNNNNGG	9 cut(s)	746, 1433, 1510, 1843, 1926, 2091, 2360, 2938, 2957
Bse118I	RCCGGY	3 cut(s)	196, 1823, 1872
Bse1I	ACTGG	4 cut(s)	792, 1270, 1306, 2808
Bse3DI	GCAATG	2 cut(s)	765, 1314
Bse8I	GATNNNNATC	2 cut(s)	918, 1221
BseBI	CCWGG	5 cut(s)	657, 855, 1389, 1637, 2087
BseGI	GGATG	5 cut(s)	542, 736, 1421, 2353, 2471
BseJI	GATNNNNATC	2 cut(s)	918, 1221
BseLI	CCNNNNNNNGG	9 cut(s)	746, 1433, 1510, 1843, 1926, 2091, 2360, 2938, 2957
BseMI	GCAATG	2 cut(s)	765, 1314
BseMII	CTCAG	2 cut(s)	1605, 2748
BseNI	ACTGG	4 cut(s)	792, 1270, 1306, 2808
BseRI	GAGGAG	2 cut(s)	2484, 2774
BseSI	GKGCMC	1 cut(s)	2591
BsgI	GTGCAG	1 cut(s)	2082
Bsh1236I	CGCG	1 cut(s)	249
BshNI	GGYRCC	1 cut(s)	1826
BshTI	ACCGGT	2 cut(s)	1823, 1872
BsiHKAI	GWGCWC	5 cut(s)	157, 405, 1033, 1872, 2084
BsiHKCI	CYCGRG	3 cut(s)	714, 1410, 2905
BsiSI	CCGG	4 cut(s)	197, 747, 1824, 1873
BsiWI	CGTACG	1 cut(s)	1577
BslFI	GGGAC	4 cut(s)	265, 982, 1749, 2103
BslI	CCNNNNNNNGG	9 cut(s)	746, 1433, 1510, 1843, 1926, 2091, 2360, 2938, 2957
BsmAI	GTCTC	1 cut(s)	1310
BsmFI	GGGAC	4 cut(s)	265, 982, 1749, 2103
BsmI	GAATGC	4 cut(s)	240, 2017, 2429, 2926
Bso31I	GGTCTC	1 cut(s)	1310
BsoBI	CYCGRG	3 cut(s)	714, 1410, 2905
Bsp1286I	GDGCHC	8 cut(s)	157, 405, 664, 1033, 1505, 1872, 2084, 2591
Bsp1407I	TGTACA	1 cut(s)	727
BspCNI	CTCAG	2 cut(s)	1606, 2749
BspFNI	CGCG	1 cut(s)	249
BspHI	TCATGA	1 cut(s)	1882
BspLI	GGNNCC	3 cut(s)	661, 1828, 2648
BspMAI	CTGCAG	3 cut(s)	349, 1510, 2625
BspMI	ACCTGC	1 cut(s)	2628
BspOI	GCTAGC	2 cut(s)	1022, 2904
BspPI	GGATC	1 cut(s)	2551
BspT107I	GGYRCC	1 cut(s)	1826
BspTI	CTTAAG	1 cut(s)	1169
BspTNI	GGTCTC	1 cut(s)	1310
BsrDI	GCAATG	2 cut(s)	765, 1314
BsrFI	RCCGGY	3 cut(s)	196, 1823, 1872
BsrGI	TGTACA	1 cut(s)	727
BsrI	ACTGG	4 cut(s)	792, 1270, 1306, 2808
BssAI	RCCGGY	3 cut(s)	196, 1823, 1872
BssNI	GRCGYC	1 cut(s)	2186
BssSI	CACGAG	2 cut(s)	2006, 2779
BssT1I	CCWWGG	4 cut(s)	609, 771, 1082, 1778
Bst2BI	CACGAG	2 cut(s)	2006, 2779
Bst2UI	CCWGG	5 cut(s)	657, 855, 1389, 1637, 2087
Bst4CI	ACNGT	7 cut(s)	54, 307, 1526, 2077, 2173, 2440, 2543
BstACI	GRCGYC	1 cut(s)	2186
BstAFI	CTTAAG	1 cut(s)	1169
BstAPI	GCANNNNNTGC	1 cut(s)	1028
BstAUI	TGTACA	1 cut(s)	727
BstBAI	YACGTR	2 cut(s)	1577, 1581
BstC8I	GCNNGC	5 cut(s)	198, 1020, 1439, 2674, 2902
BstDEI	CTNAG	3 cut(s)	627, 1614, 2757
BstDSI	CCRYGG	1 cut(s)	1891
BstEII	GGTNACC	1 cut(s)	1819
BstENI	CCTNNNNNAGG	1 cut(s)	2936
BstF5I	GGATG	5 cut(s)	542, 736, 1421, 2353, 2471
BstFNI	CGCG	1 cut(s)	249
BstH2I	RGCGCY	2 cut(s)	934, 2913
BstHHI	GCGC	4 cut(s)	933, 994, 1645, 2912
BstMAI	GTCTC	1 cut(s)	1310
BstMWI	GCNNNNNNNGC	7 cut(s)	197, 206, 246, 998, 1028, 1640, 2646
BstNI	CCWGG	5 cut(s)	657, 855, 1389, 1637, 2087
BstNSI	RCATGY	3 cut(s)	734, 2676, 2866
BstPI	GGTNACC	1 cut(s)	1819
BstSCI	CCNGG	5 cut(s)	655, 853, 1387, 1635, 2085
BstSFI	CTRYAG	6 cut(s)	345, 1157, 1506, 2439, 2621, 2701
BstSLI	GKGCMC	1 cut(s)	2591
BstSNI	TACGTA	1 cut(s)	1581
BstUI	CGCG	1 cut(s)	249
BstX2I	RGATCY	3 cut(s)	630, 2556, 2783
BstYI	RGATCY	3 cut(s)	630, 2556, 2783
BsuI	GTATCC	1 cut(s)	1514
BtgI	CCRYGG	1 cut(s)	1891
BtrI	CACGTC	2 cut(s)	337, 2360
BtsCI	GGATG	5 cut(s)	542, 736, 1421, 2353, 2471
BtsI	GCAGTG	5 cut(s)	295, 621, 1109, 1442, 2145
BtsIMutI	CAGTG	9 cut(s)	50, 295, 621, 799, 1109, 1442, 2073, 2145, 2178
BveI	ACCTGC	1 cut(s)	2628
Cac8I	GCNNGC	5 cut(s)	198, 1020, 1439, 2674, 2902
CaiI	CAGNNNCTG	3 cut(s)	515, 917, 2741
CciI	TCATGA	1 cut(s)	1882
CfoI	GCGC	4 cut(s)	933, 994, 1645, 2912
Cfr10I	RCCGGY	3 cut(s)	196, 1823, 1872
Cfr13I	GGNCC	6 cut(s)	353, 857, 1895, 2351, 2647, 2768
CseI	GACGC	2 cut(s)	2762, 2799
Csp6I	GTAC	5 cut(s)	410, 728, 1578, 1618, 1980
CspAI	ACCGGT	2 cut(s)	1823, 1872
CspCI	CAANNNNNGTGG	2 cut(s)	1036, 1071
CviQI	GTAC	5 cut(s)	410, 728, 1578, 1618, 1980
DdeI	CTNAG	3 cut(s)	627, 1614, 2757
DraIII	CACNNNGTG	1 cut(s)	488
EaeI	YGGCCR	2 cut(s)	577, 1952
Ecl136II	GAGCTC	2 cut(s)	155, 403
Eco105I	TACGTA	1 cut(s)	1581
Eco130I	CCWWGG	4 cut(s)	609, 771, 1082, 1778
Eco147I	AGGCCT	1 cut(s)	25
Eco24I	GRGCYC	4 cut(s)	157, 405, 664, 1505
Eco31I	GGTCTC	1 cut(s)	1310
Eco32I	GATATC	2 cut(s)	676, 2217
Eco47I	GGWCC	3 cut(s)	1895, 2351, 2768
Eco47III	AGCGCT	2 cut(s)	932, 2911
Eco53kI	GAGCTC	2 cut(s)	155, 403
Eco57I	CTGAAG	1 cut(s)	2888
Eco88I	CYCGRG	3 cut(s)	714, 1410, 2905
Eco91I	GGTNACC	1 cut(s)	1819
EcoICRI	GAGCTC	2 cut(s)	155, 403
EcoNI	CCTNNNNNAGG	1 cut(s)	2936
EcoO109I	RGGNCCY	1 cut(s)	2647
EcoO65I	GGTNACC	1 cut(s)	1819
EcoRII	CCWGG	5 cut(s)	655, 853, 1387, 1635, 2085
EcoRV	GATATC	2 cut(s)	676, 2217
EcoT14I	CCWWGG	4 cut(s)	609, 771, 1082, 1778
EcoT22I	ATGCAT	1 cut(s)	1003
EcoT38I	GRGCYC	4 cut(s)	157, 405, 664, 1505
ErhI	CCWWGG	4 cut(s)	609, 771, 1082, 1778
FalI	AAGNNNNNCTT	4 cut(s)	632, 664, 1163, 1195
FaqI	GGGAC	4 cut(s)	265, 982, 1749, 2103
FauI	CCCGC	2 cut(s)	242, 2950
FbaI	TGATCA	2 cut(s)	232, 1210
FblI	GTMKAC	1 cut(s)	2513
FokI	GGATG	5 cut(s)	549, 723, 1428, 2360, 2478
FriOI	GRGCYC	4 cut(s)	157, 405, 664, 1505
FspI	TGCGCA	1 cut(s)	1644
GlaI	GCGC	4 cut(s)	932, 993, 1644, 2911
GsuI	CTGGAG	3 cut(s)	678, 1305, 2468
HaeII	RGCGCY	2 cut(s)	934, 2913
HapII	CCGG	4 cut(s)	197, 747, 1824, 1873
HgaI	GACGC	2 cut(s)	2762, 2799
HhaI	GCGC	4 cut(s)	933, 994, 1645, 2912
Hin1I	GRCGYC	1 cut(s)	2186
Hin6I	GCGC	4 cut(s)	931, 992, 1643, 2910
HinP1I	GCGC	4 cut(s)	931, 992, 1643, 2910
HincII	GTYRAC	3 cut(s)	2506, 2514, 2805
HindII	GTYRAC	3 cut(s)	2506, 2514, 2805
HindIII	AAGCTT	2 cut(s)	976, 2638
HpaII	CCGG	4 cut(s)	197, 747, 1824, 1873
HphI	GGTGA	4 cut(s)	1403, 1813, 2113, 2555
Hpy166II	GTNNAC	9 cut(s)	162, 700, 1552, 1800, 1898, 2454, 2506, 2514, 2805
Hpy188I	TCNGA	9 cut(s)	144, 232, 476, 924, 1200, 1251, 1517, 2237, 2536
Hpy8I	GTNNAC	9 cut(s)	162, 700, 1552, 1800, 1898, 2454, 2506, 2514, 2805
Hpy99I	CGWCG	2 cut(s)	338, 817
HpyAV	CCTTC	9 cut(s)	658, 837, 959, 1046, 1840, 2192, 2488, 2942, 2961
HpyCH4III	ACNGT	7 cut(s)	54, 307, 1526, 2077, 2173, 2440, 2543
HpyCH4IV	ACGT	6 cut(s)	336, 1576, 1580, 2186, 2359, 2607
HpyF10VI	GCNNNNNNNGC	7 cut(s)	197, 206, 246, 998, 1028, 1640, 2646
HpyF3I	CTNAG	3 cut(s)	627, 1614, 2757
HpySE526I	ACGT	6 cut(s)	336, 1576, 1580, 2186, 2359, 2607
Hsp92I	GRCGYC	1 cut(s)	2186
HspAI	GCGC	4 cut(s)	931, 992, 1643, 2910
KroI	GCCGGC	1 cut(s)	196
KroNI	GCCGGC	1 cut(s)	198
Ksp22I	TGATCA	2 cut(s)	232, 1210
LmnI	GCTCC	8 cut(s)	152, 319, 408, 428, 659, 948, 1324, 1847
LweI	GCATC	3 cut(s)	595, 1648, 2602
MaeII	ACGT	6 cut(s)	336, 1576, 1580, 2186, 2359, 2607
MaeIII	GTNAC	6 cut(s)	488, 1396, 1472, 1675, 1819, 2187
MboII	GAAGA	7 cut(s)	236, 1036, 1825, 1987, 2516, 2689, 2954
MfeI	CAATTG	1 cut(s)	213
MflI	RGATCY	3 cut(s)	630, 2556, 2783
MhlI	GDGCHC	8 cut(s)	157, 405, 664, 1033, 1505, 1872, 2084, 2591
MlyI	GAGTC	3 cut(s)	737, 964, 1001
MmeI	TCCRAC	1 cut(s)	1648
Mph1103I	ATGCAT	1 cut(s)	1003
MroNI	GCCGGC	1 cut(s)	196
MroXI	GAANNNNTTC	2 cut(s)	1267, 1343
MslI	CAYNNNNRTG	2 cut(s)	89, 1948
MspA1I	CMGCKG	3 cut(s)	1664, 2182, 2923
MspCI	CTTAAG	1 cut(s)	1169
MspI	CCGG	4 cut(s)	197, 747, 1824, 1873
MspR9I	CCNGG	5 cut(s)	657, 855, 1389, 1637, 2087
MunI	CAATTG	1 cut(s)	213
Mva1269I	GAATGC	4 cut(s)	240, 2017, 2429, 2926
MvaI	CCWGG	5 cut(s)	657, 855, 1389, 1637, 2087
MvnI	CGCG	1 cut(s)	249
MwoI	GCNNNNNNNGC	7 cut(s)	197, 206, 246, 998, 1028, 1640, 2646
NaeI	GCCGGC	1 cut(s)	198
NgoMIV	GCCGGC	1 cut(s)	196
NheI	GCTAGC	2 cut(s)	1018, 2900
NlaIV	GGNNCC	3 cut(s)	661, 1828, 2648
NmuCI	GTSAC	3 cut(s)	488, 1819, 2187
NsbI	TGCGCA	1 cut(s)	1644
NsiI	ATGCAT	1 cut(s)	1003
NspI	RCATGY	3 cut(s)	734, 2676, 2866
PaeI	GCATGC	1 cut(s)	2676
PagI	TCATGA	1 cut(s)	1882
PasI	CCCWGGG	1 cut(s)	2086
PceI	AGGCCT	1 cut(s)	25
PciI	ACATGT	1 cut(s)	730
PctI	GAATGC	4 cut(s)	240, 2017, 2429, 2926
PdiI	GCCGGC	1 cut(s)	198
PdmI	GAANNNNTTC	2 cut(s)	1267, 1343
Pfl23II	CGTACG	1 cut(s)	1577
PflMI	CCANNNNNTGG	2 cut(s)	1926, 2360
PfoI	TCCNGGA	1 cut(s)	655
PinAI	ACCGGT	2 cut(s)	1823, 1872
PleI	GAGTC	3 cut(s)	737, 964, 1001
PpsI	GAGTC	3 cut(s)	737, 964, 1001
Ppu21I	YACGTR	2 cut(s)	1577, 1581
PscI	ACATGT	1 cut(s)	730
Psp124BI	GAGCTC	2 cut(s)	157, 405
Psp6I	CCWGG	5 cut(s)	655, 853, 1387, 1635, 2085
PspEI	GGTNACC	1 cut(s)	1819
PspGI	CCWGG	5 cut(s)	655, 853, 1387, 1635, 2085
PspLI	CGTACG	1 cut(s)	1577
PspN4I	GGNNCC	3 cut(s)	661, 1828, 2648
PspPI	GGNCC	6 cut(s)	353, 857, 1895, 2351, 2647, 2768
PstI	CTGCAG	3 cut(s)	349, 1510, 2625
PstNI	CAGNNNCTG	3 cut(s)	515, 917, 2741
PsuI	RGATCY	3 cut(s)	630, 2556, 2783
PvuII	CAGCTG	3 cut(s)	1664, 2182, 2923
RsaI	GTAC	5 cut(s)	411, 729, 1579, 1619, 1981
RsaNI	GTAC	5 cut(s)	410, 728, 1578, 1618, 1980
RseI	CAYNNNNRTG	2 cut(s)	89, 1948
SacI	GAGCTC	2 cut(s)	157, 405
SalI	GTCGAC	1 cut(s)	2512
Sau96I	GGNCC	6 cut(s)	353, 857, 1895, 2351, 2647, 2768
SbfI	CCTGCAGG	1 cut(s)	1510
SchI	GAGTC	3 cut(s)	737, 964, 1001
ScrFI	CCNGG	5 cut(s)	657, 855, 1389, 1637, 2087
SdaI	CCTGCAGG	1 cut(s)	1510
SduI	GDGCHC	8 cut(s)	157, 405, 664, 1033, 1505, 1872, 2084, 2591
SfaNI	GCATC	3 cut(s)	595, 1648, 2602
SfcI	CTRYAG	6 cut(s)	345, 1157, 1506, 2439, 2621, 2701
SgrAI	CRCCGGYG	1 cut(s)	1823
SinI	GGWCC	3 cut(s)	1895, 2351, 2768
SmiMI	CAYNNNNRTG	2 cut(s)	89, 1948
SmlI	CTYRAG	3 cut(s)	1169, 1790, 1865
SmoI	CTYRAG	3 cut(s)	1169, 1790, 1865
SnaBI	TACGTA	1 cut(s)	1581
SpeI	ACTAGT	1 cut(s)	2879
SphI	GCATGC	1 cut(s)	2676
Sse8387I	CCTGCAGG	1 cut(s)	1510
SseBI	AGGCCT	1 cut(s)	25
SspI	AATATT	3 cut(s)	148, 1255, 2026
SstI	GAGCTC	2 cut(s)	157, 405
StuI	AGGCCT	1 cut(s)	25
StyD4I	CCNGG	5 cut(s)	655, 853, 1387, 1635, 2085
StyI	CCWWGG	4 cut(s)	609, 771, 1082, 1778
TaaI	ACNGT	7 cut(s)	54, 307, 1526, 2077, 2173, 2440, 2543
TaiI	ACGT	6 cut(s)	339, 1579, 1583, 2189, 2362, 2610
TaqI	TCGA	4 cut(s)	1102, 1465, 2230, 2513
TaqII	GACCGA	1 cut(s)	1300
TatI	WGTACW	3 cut(s)	727, 1617, 1979
TscAI	CASTG	9 cut(s)	57, 295, 621, 799, 1116, 1449, 2080, 2152, 2178
TseFI	GTSAC	3 cut(s)	488, 1819, 2187
Tsp45I	GTSAC	3 cut(s)	488, 1819, 2187
TspDTI	ATGAA	7 cut(s)	32, 696, 725, 978, 1190, 2297, 2398
TspGWI	ACGGA	5 cut(s)	830, 887, 1880, 2206, 2360
TspRI	CASTG	9 cut(s)	57, 295, 621, 799, 1116, 1449, 2080, 2152, 2178
Van91I	CCANNNNNTGG	2 cut(s)	1926, 2360
Vha464I	CTTAAG	1 cut(s)	1169
VpaK11BI	GGWCC	3 cut(s)	1895, 2351, 2768
XagI	CCTNNNNNAGG	1 cut(s)	2936
XapI	RAATTY	3 cut(s)	293, 1294, 1652
XbaI	TCTAGA	3 cut(s)	941, 2297, 2786
XceI	RCATGY	3 cut(s)	734, 2676, 2866
XcmI	CCANNNNNNNNNTGG	1 cut(s)	91
XmiI	GTMKAC	1 cut(s)	2513
XmnI	GAANNNNTTC	2 cut(s)	1267, 1343
ZraI	GACGTC	1 cut(s)	2187
Zsp2I	ATGCAT	1 cut(s)	1003

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.