MD09G1251600.v1.1

Bifunctional epoxide hydrolase 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0001763
Physical Location & Seq
Reverse (-)
32264768 .. 32267108
2341 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1251600.v1.1.491

Sequence Viewer

Length: 945 bp
ATGATGGAAAATATCCAGCACAAACATGTGGAAGTAAGAGGACTCAAGCTTCACGTAGCCGAAATCGGAAGTGGTCCAAAGGCTGTGGTGTTCCTTCATGGATTTCCAGAAATATGGTACACGTGGAGGCATCAAATGGTTGCTGTAGCGGATAGAGGCTACCGGGCATTAACCATTGATTTCAGGGGCTATGGACTTTCCGAGCAGCCGGCTGACCCTGAGAAGGACACCTTCATCGACCTTGTCCACGATATCGTTGCCCTTTTGGATTTTTTGGTCATTAACAAGGCTTTTCTTATTGGGAAAGATTTTGGAGCATTTCCTGCATACATGGTAGCTGCTCTCCACCCAGAGCGGGTATCTGGCGTCGTTACACTAGGTGTTCCTTTCATGCTACCAGGTCCCTCTGCCATCCAAAATCATCTCCTTCCTGAAGGCTTCTACATGTCAAGGTGGCAGGAGCCAGGGCGGGCAGAAGCAGATTTTGGCCGCTTTGATGTGAAGACGGTGATTAGGAACATCTACATTCTCTTCTCTAGAAGCGAGATCCCAATAGCTGCCCCTGATCAAGAAATCATGGACCTGTTTGACCCTGCAACTCCTCTGCCGCCAGGGTTTTCGGAAGAAGATCTTGATGTCTACGCATCTTTGTATGAGAAATCTGGATTCCGTTATCCATTGCGAGTTCCATACAGAACCTTGGCCGTGGATTGTGGTATAACCGATCCCAAAGTCTCGACTCCATCTCTGCTGATCGTGGGCGAGAAGGATTACGTCTTAAAGTTTCCGGGGATGGAGGACTACATCCGCACTGGGGCAGTGAAAAACTTTGTGCCGGATCTGGAGATTACATTCATCGAAGAAGGGAGCCATTTTGTGCATGAACAACTTGTGGAGAAAGTTAACCAGCTGATCCTCGCATTCCTTGACAAACATGCTATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000287 GO:0001676 GO:0003008 GO:0003013 GO:0003018 GO:0003674 GO:0003824 GO:0004301 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005782 GO:0005829 GO:0006082 GO:0006605 GO:0006625 GO:0006629 GO:0006631 GO:0006633 GO:0006690 GO:0006725 GO:0006793 GO:0006796 GO:0006805 GO:0006810 GO:0006873 GO:0006874 GO:0006875 GO:0006886 GO:0006950 GO:0006952 GO:0006954 GO:0006996 GO:0007031 GO:0008015 GO:0008104 GO:0008150 GO:0008152 GO:0008217 GO:0008610 GO:0009056 GO:0009058 GO:0009410 GO:0009636 GO:0009810 GO:0009893 GO:0009987 GO:0010468 GO:0010604 GO:0010628 GO:0015031 GO:0015643 GO:0015833 GO:0016043 GO:0016053 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0016801 GO:0016803 GO:0017144 GO:0018904 GO:0019216 GO:0019218 GO:0019222 GO:0019369 GO:0019373 GO:0019439 GO:0019725 GO:0019752 GO:0030003 GO:0030258 GO:0031907 GO:0031974 GO:0032501 GO:0032787 GO:0033036 GO:0033365 GO:0033559 GO:0034613 GO:0035150 GO:0035296 GO:0042221 GO:0042577 GO:0042578 GO:0042579 GO:0042592 GO:0042632 GO:0042759 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043574 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046272 GO:0046394 GO:0046483 GO:0046839 GO:0046872 GO:0046907 GO:0046983 GO:0048518 GO:0048878 GO:0050789 GO:0050801 GO:0050880 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0055088 GO:0055092 GO:0060255 GO:0062012 GO:0065007 GO:0065008 GO:0070013 GO:0070727 GO:0070887 GO:0071466 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072330 GO:0072503 GO:0072507 GO:0072593 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0090066 GO:0090181 GO:0097176 GO:0097746 GO:0097755 GO:0098771 GO:1900673 GO:1901360 GO:1901361 GO:1901568 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

315

Amino Acids

35.42

Weight (kDa)

5.21

Isoelectric Point (pI)

41.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 27 - 128 5e-10 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 28 - 135 3.1e-21 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 29 - 299 8.7e-16 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 355
AccI GTMKAC 1 cut(s) 639
AciI CCGC 6 cut(s) 149, 355, 469, 490, 608, 808
AclWI GGATC 4 cut(s) 541, 719, 846, 907
AcoI YGGCCR 2 cut(s) 487, 702
AcuI CTGAAG 1 cut(s) 453
AcvI CACGTG 1 cut(s) 123
AcyI GRCGYC 1 cut(s) 366
AdeI CACNNNGTG 1 cut(s) 380
AfaI GTAC 1 cut(s) 119
AfiI CCNNNNNNNGG 3 cut(s) 223, 355, 814
AflIII ACRYGT 3 cut(s) 25, 120, 444
AjnI CCWGG 3 cut(s) 397, 463, 610
AjuI GAANNNNNNNTTGG 2 cut(s) 468, 500
AluBI AGCT 4 cut(s) 49, 338, 557, 910
AluI AGCT 4 cut(s) 49, 338, 557, 910
Alw26I GTCTC 1 cut(s) 739
AlwI GGATC 4 cut(s) 541, 719, 846, 907
AoxI GGCC 2 cut(s) 487, 702
ApeKI GCWGC 3 cut(s) 205, 338, 557
AspS9I GGNCC 3 cut(s) 74, 401, 580
AsuC2I CCSGG 2 cut(s) 164, 789
AsuHPI GGTGA 1 cut(s) 520
AvaII GGWCC 3 cut(s) 74, 401, 580
BbrPI CACGTG 1 cut(s) 123
BbsI GAAGAC 1 cut(s) 509
BbvI GCAGC 3 cut(s) 217, 325, 544
BccI CCATC 3 cut(s) 419, 751, 787
BceAI ACGGC 1 cut(s) 689
BcgI CGANNNNNNTGC 2 cut(s) 239, 273
BciT130I CCWGG 3 cut(s) 399, 465, 612
BclI TGATCA 1 cut(s) 565
BcnI CCSGG 2 cut(s) 164, 789
BcoDI GTCTC 1 cut(s) 739
BfaI CTAG 2 cut(s) 377, 537
BfmI CTRYAG 1 cut(s) 144
BglII AGATCT 1 cut(s) 628
BisI GCNGC 5 cut(s) 206, 339, 490, 558, 608
BlsI GCNGC 5 cut(s) 207, 340, 491, 559, 609
Bme1390I CCNGG 5 cut(s) 164, 399, 465, 612, 789
Bme18I GGWCC 3 cut(s) 74, 401, 580
BmgT120I GGNCC 3 cut(s) 74, 401, 580
BmiI GGNNCC 3 cut(s) 403, 462, 869
BmrFI CCNGG 5 cut(s) 164, 399, 465, 612, 789
BmrI ACTGGG 1 cut(s) 822
BmsI GCATC 2 cut(s) 139, 653
BmuI ACTGGG 1 cut(s) 822
BpiI GAAGAC 1 cut(s) 509
BpmI CTGGAG 1 cut(s) 863
BpuEI CTTGAG 1 cut(s) 29
BpuMI CCSGG 2 cut(s) 164, 789
BsaAI YACGTR 2 cut(s) 55, 123
BsaHI GRCGYC 1 cut(s) 366
BsaJI CCNNGG 5 cut(s) 464, 611, 699, 705, 788
Bsc4I CCNNNNNNNGG 3 cut(s) 223, 355, 814
Bse118I RCCGGY 1 cut(s) 208
Bse1I ACTGG 1 cut(s) 817
Bse3DI GCAATG 1 cut(s) 677
BseBI CCWGG 3 cut(s) 399, 465, 612
BseDI CCNNGG 5 cut(s) 464, 611, 699, 705, 788
BseGI GGATG 3 cut(s) 411, 798, 804
BseLI CCNNNNNNNGG 3 cut(s) 223, 355, 814
BseMI GCAATG 1 cut(s) 677
BseMII CTCAG 1 cut(s) 210
BseNI ACTGG 1 cut(s) 817
BseRI GAGGAG 1 cut(s) 591
BseXI GCAGC 3 cut(s) 217, 325, 544
BshFI GGCC 2 cut(s) 489, 704
BsiSI CCGG 4 cut(s) 163, 209, 788, 836
BslFI GGGAC 1 cut(s) 387
BslI CCNNNNNNNGG 3 cut(s) 223, 355, 814
BsmAI GTCTC 1 cut(s) 739
BsmFI GGGAC 1 cut(s) 387
BsmI GAATGC 1 cut(s) 920
BsnI GGCC 2 cut(s) 489, 704
Bsp143I GATC 7 cut(s) 546, 565, 628, 724, 753, 838, 912
BspACI CCGC 6 cut(s) 149, 355, 469, 490, 608, 808
BspANI GGCC 2 cut(s) 489, 704
BspCNI CTCAG 1 cut(s) 211
BspLI GGNNCC 3 cut(s) 403, 462, 869
BspPI GGATC 4 cut(s) 541, 719, 846, 907
BsrBI CCGCTC 1 cut(s) 355
BsrDI GCAATG 1 cut(s) 677
BsrFI RCCGGY 1 cut(s) 208
BsrI ACTGG 1 cut(s) 817
BssAI RCCGGY 1 cut(s) 208
BssECI CCNNGG 5 cut(s) 464, 611, 699, 705, 788
BssMI GATC 7 cut(s) 546, 565, 628, 724, 753, 838, 912
BssNI GRCGYC 1 cut(s) 366
BssT1I CCWWGG 1 cut(s) 699
Bst2UI CCWGG 3 cut(s) 399, 465, 612
Bst4CI ACNGT 1 cut(s) 508
Bst6I CTCTTC 1 cut(s) 536
BstACI GRCGYC 1 cut(s) 366
BstAPI GCANNNNNTGC 1 cut(s) 323
BstBAI YACGTR 2 cut(s) 55, 123
BstC8I GCNNGC 2 cut(s) 210, 471
BstDEI CTNAG 1 cut(s) 219
BstDSI CCRYGG 1 cut(s) 705
BstF5I GGATG 3 cut(s) 411, 798, 804
BstKTI GATC 7 cut(s) 549, 568, 631, 727, 756, 841, 915
BstMAI GTCTC 1 cut(s) 739
BstMBI GATC 7 cut(s) 546, 565, 628, 724, 753, 838, 912
BstMWI GCNNNNNNNGC 1 cut(s) 323
BstNI CCWGG 3 cut(s) 399, 465, 612
BstNSI RCATGY 3 cut(s) 29, 448, 938
BstSCI CCNGG 5 cut(s) 162, 397, 463, 610, 787
BstSFI CTRYAG 1 cut(s) 144
BstV1I GCAGC 3 cut(s) 217, 325, 544
BstV2I GAAGAC 1 cut(s) 509
BstX2I RGATCY 3 cut(s) 546, 628, 838
BstXI CCANNNNNNTGG 1 cut(s) 114
BstYI RGATCY 3 cut(s) 546, 628, 838
BsuRI GGCC 2 cut(s) 489, 704
BtgI CCRYGG 1 cut(s) 705
BtsCI GGATG 3 cut(s) 411, 798, 804
BtsI GCAGTG 1 cut(s) 825
BtsIMutI CAGTG 2 cut(s) 810, 825
Cac8I GCNNGC 2 cut(s) 210, 471
Cfr10I RCCGGY 1 cut(s) 208
Cfr13I GGNCC 3 cut(s) 74, 401, 580
CseI GACGC 1 cut(s) 355
CsiI ACCWGGT 1 cut(s) 397
Csp6I GTAC 1 cut(s) 118
CspCI CAANNNNNGTGG 2 cut(s) 66, 101
CviAII CATG 8 cut(s) 26, 98, 331, 391, 445, 577, 881, 935
CviQI GTAC 1 cut(s) 118
DdeI CTNAG 1 cut(s) 219
DpnI GATC 7 cut(s) 548, 567, 630, 726, 755, 840, 914
DpnII GATC 7 cut(s) 546, 565, 628, 724, 753, 838, 912
DraIII CACNNNGTG 1 cut(s) 380
EaeI YGGCCR 2 cut(s) 487, 702
Eam1104I CTCTTC 1 cut(s) 536
EarI CTCTTC 1 cut(s) 536
Eco130I CCWWGG 1 cut(s) 699
Eco32I GATATC 1 cut(s) 253
Eco47I GGWCC 3 cut(s) 74, 401, 580
Eco57I CTGAAG 1 cut(s) 453
Eco72I CACGTG 1 cut(s) 123
EcoO109I RGGNCCY 1 cut(s) 401
EcoRII CCWGG 3 cut(s) 397, 463, 610
EcoRV GATATC 1 cut(s) 253
EcoT14I CCWWGG 1 cut(s) 699
ErhI CCWWGG 1 cut(s) 699
FaeI CATG 8 cut(s) 29, 101, 334, 394, 448, 580, 884, 938
FalI AAGNNNNNCTT 4 cut(s) 215, 247, 615, 647
FaqI GGGAC 1 cut(s) 387
FatI CATG 8 cut(s) 25, 97, 330, 390, 444, 576, 880, 934
FauI CCCGC 2 cut(s) 348, 462
FbaI TGATCA 1 cut(s) 565
FblI GTMKAC 1 cut(s) 639
Fnu4HI GCNGC 5 cut(s) 206, 339, 490, 558, 608
FokI GGATG 3 cut(s) 398, 791, 805
Fsp4HI GCNGC 5 cut(s) 206, 339, 490, 558, 608
FspBI CTAG 2 cut(s) 377, 537
GluI GCNGC 5 cut(s) 206, 339, 490, 558, 608
GsuI CTGGAG 1 cut(s) 863
HaeIII GGCC 2 cut(s) 489, 704
HapII CCGG 4 cut(s) 163, 209, 788, 836
HgaI GACGC 1 cut(s) 355
Hin1I GRCGYC 1 cut(s) 366
Hin1II CATG 8 cut(s) 29, 101, 334, 394, 448, 580, 884, 938
HincII GTYRAC 1 cut(s) 904
HindII GTYRAC 1 cut(s) 904
HindIII AAGCTT 1 cut(s) 47
HinfI GANTC 3 cut(s) 42, 666, 739
HpaI GTTAAC 1 cut(s) 904
HpaII CCGG 4 cut(s) 163, 209, 788, 836
HphI GGTGA 1 cut(s) 520
Hpy166II GTNNAC 4 cut(s) 120, 247, 640, 904
Hpy188I TCNGA 4 cut(s) 68, 202, 622, 944
Hpy188III TCNNGA 8 cut(s) 107, 431, 537, 569, 632, 663, 736, 842
Hpy8I GTNNAC 4 cut(s) 120, 247, 640, 904
Hpy99I CGWCG 1 cut(s) 371
HpyAV CCTTC 7 cut(s) 104, 217, 241, 428, 437, 760, 857
HpyCH4III ACNGT 1 cut(s) 508
HpyCH4IV ACGT 3 cut(s) 54, 122, 774
HpyCH4V TGCA 3 cut(s) 326, 596, 880
HpyF10VI GCNNNNNNNGC 1 cut(s) 323
HpyF3I CTNAG 1 cut(s) 219
HpySE526I ACGT 3 cut(s) 54, 122, 774
Hsp92I GRCGYC 1 cut(s) 366
Hsp92II CATG 8 cut(s) 29, 101, 334, 394, 448, 580, 884, 938
KroI GCCGGC 1 cut(s) 208
KroNI GCCGGC 1 cut(s) 210
Ksp22I TGATCA 1 cut(s) 565
KspAI GTTAAC 1 cut(s) 904
Kzo9I GATC 7 cut(s) 546, 565, 628, 724, 753, 838, 912
LmnI GCTCC 3 cut(s) 314, 460, 867
Lsp1109I GCAGC 3 cut(s) 217, 325, 544
LweI GCATC 2 cut(s) 139, 653
MabI ACCWGGT 1 cut(s) 397
MaeI CTAG 2 cut(s) 377, 537
MaeII ACGT 3 cut(s) 54, 122, 774
MaeIII GTNAC 1 cut(s) 370
MalI GATC 7 cut(s) 548, 567, 630, 726, 755, 840, 914
MbiI CCGCTC 1 cut(s) 355
MboI GATC 7 cut(s) 546, 565, 628, 724, 753, 838, 912
MboII GAAGA 5 cut(s) 514, 523, 635, 638, 872
MflI RGATCY 3 cut(s) 546, 628, 838
MlyI GAGTC 2 cut(s) 36, 733
MnlI CCTC 7 cut(s) 32, 120, 149, 415, 612, 790, 926
MroNI GCCGGC 1 cut(s) 208
MseI TTAA 4 cut(s) 170, 282, 779, 903
MslI CAYNNNNRTG 1 cut(s) 24
MspA1I CMGCKG 1 cut(s) 910
MspI CCGG 4 cut(s) 163, 209, 788, 836
MspR9I CCNGG 5 cut(s) 164, 399, 465, 612, 789
Mva1269I GAATGC 1 cut(s) 920
MvaI CCWGG 3 cut(s) 399, 465, 612
MwoI GCNNNNNNNGC 1 cut(s) 323
NaeI GCCGGC 1 cut(s) 210
NciI CCSGG 2 cut(s) 164, 789
NdeII GATC 7 cut(s) 546, 565, 628, 724, 753, 838, 912
NgoMIV GCCGGC 1 cut(s) 208
NlaIII CATG 8 cut(s) 29, 101, 334, 394, 448, 580, 884, 938
NlaIV GGNNCC 3 cut(s) 403, 462, 869
NspI RCATGY 3 cut(s) 29, 448, 938
PciI ACATGT 2 cut(s) 25, 444
PctI GAATGC 1 cut(s) 920
PdiI GCCGGC 1 cut(s) 210
PfeI GAWTC 1 cut(s) 666
PflFI GACNNNGTC 1 cut(s) 242
PkrI GCNGC 5 cut(s) 207, 340, 491, 559, 609
PleI GAGTC 2 cut(s) 36, 733
PmaCI CACGTG 1 cut(s) 123
PmlI CACGTG 1 cut(s) 123
PpsI GAGTC 2 cut(s) 36, 733
Ppu21I YACGTR 2 cut(s) 55, 123
PpuMI RGGWCCY 1 cut(s) 401
PscI ACATGT 2 cut(s) 25, 444
Psp5II RGGWCCY 1 cut(s) 401
Psp6I CCWGG 3 cut(s) 397, 463, 610
PspCI CACGTG 1 cut(s) 123
PspGI CCWGG 3 cut(s) 397, 463, 610
PspN4I GGNNCC 3 cut(s) 403, 462, 869
PspPI GGNCC 3 cut(s) 74, 401, 580
PspPPI RGGWCCY 1 cut(s) 401
PsuI RGATCY 3 cut(s) 546, 628, 838
PsyI GACNNNGTC 1 cut(s) 242
PvuII CAGCTG 1 cut(s) 910
RsaI GTAC 1 cut(s) 119
RsaNI GTAC 1 cut(s) 118
RseI CAYNNNNRTG 1 cut(s) 24
SaqAI TTAA 4 cut(s) 170, 282, 779, 903
SatI GCNGC 5 cut(s) 206, 339, 490, 558, 608
Sau3AI GATC 7 cut(s) 546, 565, 628, 724, 753, 838, 912
Sau96I GGNCC 3 cut(s) 74, 401, 580
SchI GAGTC 2 cut(s) 36, 733
ScrFI CCNGG 5 cut(s) 164, 399, 465, 612, 789
SexAI ACCWGGT 1 cut(s) 397
SfaNI GCATC 2 cut(s) 139, 653
SfcI CTRYAG 1 cut(s) 144
SinI GGWCC 3 cut(s) 74, 401, 580
SmiMI CAYNNNNRTG 1 cut(s) 24
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
SsiI CCGC 6 cut(s) 149, 355, 469, 490, 608, 808
SspMI CTAG 2 cut(s) 377, 537
StyD4I CCNGG 5 cut(s) 162, 397, 463, 610, 787
StyI CCWWGG 1 cut(s) 699
TaaI ACNGT 1 cut(s) 508
TaiI ACGT 3 cut(s) 57, 125, 777
TaqI TCGA 3 cut(s) 237, 737, 858
TauI GCSGC 2 cut(s) 492, 610
TfiI GAWTC 1 cut(s) 666
Tru1I TTAA 4 cut(s) 170, 282, 779, 903
Tru9I TTAA 4 cut(s) 170, 282, 779, 903
TscAI CASTG 2 cut(s) 817, 825
TseI GCWGC 3 cut(s) 205, 338, 557
TspDTI ATGAA 5 cut(s) 86, 223, 379, 844, 897
TspGWI ACGGA 1 cut(s) 659
TspRI CASTG 2 cut(s) 817, 825
Tth111I GACNNNGTC 1 cut(s) 242
VpaK11BI GGWCC 3 cut(s) 74, 401, 580
XbaI TCTAGA 1 cut(s) 536
XceI RCATGY 3 cut(s) 29, 448, 938
XmiI GTMKAC 1 cut(s) 639
XspI CTAG 2 cut(s) 377, 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.