Prupe.3G156600_v2.0.a1

Bifunctional epoxide hydrolase 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0001763
Physical Location & Seq
Reverse (-)
17503392 .. 17509706
6315 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G156600.2

Sequence Viewer

Length: 948 bp
ATGGAACAGATCCAGCATAGACATGTGCAAGTGAGAGGTGTAAAGCTTCATGTAGCTGAAATTGGAAGCGGTCCAAAAGTGGTGCTTTTCTTACATGGGTTTCCGGAAATATGGTACACATGGAGGCATCAAATGGTTGCTGTGGCAAACAAAGGCCACCGTGCCATAGCCATTGATTTCAGGGGCTATGGACTTTCTGAGCAGCCAGCGGAACCAGAAAAGGCAACCTTCAAAGACCTCATTGACGATGTTGTTGCCCTTTTGGATTCTTTGAGCATTGACAAGGCTGTCCTTGTTGGGAAGGATTTTGGAGCTTTTCCTGCATACGTAATACCTGCTCTCCACCCACAGCGGGTATCGGGCATGATAACAGTAGGTGTTCCTTTCATGCAACCAGATGCTTCTCCTGTCCAATTTCATCTCCTCCCAAAAGGATTCTACATGTCAAGGTTGAAGGAGCCAGGGCGGGCAGAAGCAGATTTTGGCCGCTTTGATGTGAAGACGGTGATACGGAACATCTACATTCTCTTCTCCGGAAGTGAGCTCCAAGTGGCTGCTGATGATCAAGAAATCATGGACTTGGTTGACCCTGCAACTCCTCTACCACCATGGTTCTCTGAGGAAGATCTTGCCGCCTATGCATCTTTATATGAGAAATCCGGACTCCATTTTCCATTGCAAATTCCATACAGATCCAGACGTTCATCCGAGGATTTCCATTTAACTGATCCAAAAATCTCAGCTCCATCGCTGCTTATCATGGGTGAGAAGGACTACTTCTTAAAGTTTCCGGGGGTCGAGGACTACTTGCGCACTGGGGCAGTGAAGCATTTTGTGCCTGATTTGGACATTAATTTCATTGCAGAAGGGACTCACTTTGTACAGGAACAATTTCCGGAGCAAGTTAATCAGCTAATCACCAGCTTCCTGGACAAACATGGCATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000287 GO:0001676 GO:0003008 GO:0003013 GO:0003018 GO:0003674 GO:0003824 GO:0004301 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005782 GO:0005829 GO:0006082 GO:0006605 GO:0006625 GO:0006629 GO:0006631 GO:0006633 GO:0006690 GO:0006725 GO:0006793 GO:0006796 GO:0006805 GO:0006810 GO:0006873 GO:0006874 GO:0006875 GO:0006886 GO:0006950 GO:0006952 GO:0006954 GO:0006996 GO:0007031 GO:0008015 GO:0008104 GO:0008150 GO:0008152 GO:0008217 GO:0008610 GO:0009056 GO:0009058 GO:0009410 GO:0009636 GO:0009810 GO:0009893 GO:0009987 GO:0010468 GO:0010604 GO:0010628 GO:0015031 GO:0015643 GO:0015833 GO:0016043 GO:0016053 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0016801 GO:0016803 GO:0017144 GO:0018904 GO:0019216 GO:0019218 GO:0019222 GO:0019369 GO:0019373 GO:0019439 GO:0019725 GO:0019752 GO:0030003 GO:0030258 GO:0031907 GO:0031974 GO:0032501 GO:0032787 GO:0033036 GO:0033365 GO:0033559 GO:0034613 GO:0035150 GO:0035296 GO:0042221 GO:0042577 GO:0042578 GO:0042579 GO:0042592 GO:0042632 GO:0042759 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043574 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046272 GO:0046394 GO:0046483 GO:0046839 GO:0046872 GO:0046907 GO:0046983 GO:0048518 GO:0048878 GO:0050789 GO:0050801 GO:0050880 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0055088 GO:0055092 GO:0060255 GO:0062012 GO:0065007 GO:0065008 GO:0070013 GO:0070727 GO:0070887 GO:0071466 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072330 GO:0072503 GO:0072507 GO:0072593 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0090066 GO:0090181 GO:0097176 GO:0097746 GO:0097755 GO:0098771 GO:1900673 GO:1901360 GO:1901361 GO:1901568 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

316

Amino Acids

35.49

Weight (kDa)

5.6

Isoelectric Point (pI)

42.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 287
Acc16I TGCGCA 1 cut(s) 812
Acc36I ACCTGC 1 cut(s) 343
AccIII TCCGGA 4 cut(s) 103, 533, 659, 895
AciI CCGC 6 cut(s) 69, 209, 352, 466, 487, 633
AclWI GGATC 3 cut(s) 4, 687, 722
AcoI YGGCCR 1 cut(s) 484
AcsI RAATTY 1 cut(s) 681
AfaI GTAC 2 cut(s) 116, 882
AfiI CCNNNNNNNGG 1 cut(s) 352
AflIII ACRYGT 2 cut(s) 22, 441
AgsI TTSAA 2 cut(s) 232, 454
AjnI CCWGG 2 cut(s) 460, 927
AjuI GAANNNNNNNTTGG 2 cut(s) 465, 497
AluBI AGCT 7 cut(s) 46, 56, 314, 544, 743, 913, 924
AluI AGCT 7 cut(s) 46, 56, 314, 544, 743, 913, 924
Alw21I GWGCWC 1 cut(s) 546
AlwI GGATC 3 cut(s) 4, 687, 722
Aor13HI TCCGGA 4 cut(s) 103, 533, 659, 895
AoxI GGCC 2 cut(s) 154, 484
ApeKI GCWGC 3 cut(s) 202, 554, 751
ApoI RAATTY 1 cut(s) 681
AseI ATTAAT 1 cut(s) 852
Asp700I GAANNNNTTC 1 cut(s) 891
AspLEI GCGC 1 cut(s) 813
AspS9I GGNCC 1 cut(s) 71
AsuC2I CCSGG 1 cut(s) 792
AsuHPI GGTGA 3 cut(s) 517, 776, 910
AvaII GGWCC 1 cut(s) 71
BanII GRGCYC 1 cut(s) 546
BbsI GAAGAC 1 cut(s) 506
Bbv12I GWGCWC 1 cut(s) 546
BbvI GCAGC 3 cut(s) 214, 541, 738
BccI CCATC 1 cut(s) 754
BcgI CGANNNNNNTGC 2 cut(s) 236, 270
BciT130I CCWGG 2 cut(s) 462, 929
BclI TGATCA 1 cut(s) 562
BcnI CCSGG 1 cut(s) 792
BfuAI ACCTGC 1 cut(s) 343
BglII AGATCT 1 cut(s) 625
BisI GCNGC 5 cut(s) 203, 487, 555, 633, 752
BlsI GCNGC 5 cut(s) 204, 488, 556, 634, 753
Bme1390I CCNGG 3 cut(s) 462, 792, 929
Bme18I GGWCC 1 cut(s) 71
BmgT120I GGNCC 1 cut(s) 71
BmiI GGNNCC 2 cut(s) 213, 459
BmrFI CCNGG 3 cut(s) 462, 792, 929
BmrI ACTGGG 1 cut(s) 825
BmsI GCATC 3 cut(s) 136, 388, 650
BmuI ACTGGG 1 cut(s) 825
BpiI GAAGAC 1 cut(s) 506
BpuMI CCSGG 1 cut(s) 792
BsaAI YACGTR 1 cut(s) 328
BsaJI CCNNGG 4 cut(s) 461, 608, 708, 791
BsaWI WCCGGW 4 cut(s) 103, 533, 659, 895
BsaXI ACNNNNNCTCC 2 cut(s) 324, 354
Bsc4I CCNNNNNNNGG 1 cut(s) 352
Bse1I ACTGG 1 cut(s) 820
Bse3DI GCAATG 2 cut(s) 674, 858
BseAI TCCGGA 4 cut(s) 103, 533, 659, 895
BseBI CCWGG 2 cut(s) 462, 929
BseDI CCNNGG 4 cut(s) 461, 608, 708, 791
BseGI GGATG 1 cut(s) 704
BseLI CCNNNNNNNGG 1 cut(s) 352
BseMI GCAATG 2 cut(s) 674, 858
BseMII CTCAG 3 cut(s) 189, 609, 753
BseNI ACTGG 1 cut(s) 820
BseRI GAGGAG 2 cut(s) 413, 588
BseXI GCAGC 3 cut(s) 214, 541, 738
BshFI GGCC 2 cut(s) 156, 486
BsiHKAI GWGCWC 1 cut(s) 546
BsiSI CCGG 5 cut(s) 104, 534, 660, 791, 896
BslFI GGGAC 1 cut(s) 883
BslI CCNNNNNNNGG 1 cut(s) 352
BsmFI GGGAC 1 cut(s) 883
BsnI GGCC 2 cut(s) 156, 486
Bsp1286I GDGCHC 1 cut(s) 546
Bsp13I TCCGGA 4 cut(s) 103, 533, 659, 895
Bsp1407I TGTACA 1 cut(s) 880
Bsp143I GATC 5 cut(s) 9, 562, 625, 692, 727
Bsp19I CCATGG 1 cut(s) 608
BspACI CCGC 6 cut(s) 69, 209, 352, 466, 487, 633
BspANI GGCC 2 cut(s) 156, 486
BspCNI CTCAG 3 cut(s) 190, 610, 752
BspEI TCCGGA 4 cut(s) 103, 533, 659, 895
BspLI GGNNCC 2 cut(s) 213, 459
BspMI ACCTGC 1 cut(s) 343
BspPI GGATC 3 cut(s) 4, 687, 722
BsrDI GCAATG 2 cut(s) 674, 858
BsrGI TGTACA 1 cut(s) 880
BsrI ACTGG 1 cut(s) 820
BssECI CCNNGG 4 cut(s) 461, 608, 708, 791
BssMI GATC 5 cut(s) 9, 562, 625, 692, 727
BssT1I CCWWGG 1 cut(s) 608
Bst2UI CCWGG 2 cut(s) 462, 929
Bst4CI ACNGT 3 cut(s) 161, 373, 505
Bst6I CTCTTC 1 cut(s) 533
BstAPI GCANNNNNTGC 1 cut(s) 835
BstAUI TGTACA 1 cut(s) 880
BstBAI YACGTR 1 cut(s) 328
BstC8I GCNNGC 2 cut(s) 207, 468
BstDEI CTNAG 3 cut(s) 198, 618, 739
BstDSI CCRYGG 1 cut(s) 608
BstF5I GGATG 1 cut(s) 704
BstHHI GCGC 1 cut(s) 813
BstKTI GATC 5 cut(s) 12, 565, 628, 695, 730
BstMBI GATC 5 cut(s) 9, 562, 625, 692, 727
BstMWI GCNNNNNNNGC 3 cut(s) 320, 638, 835
BstNI CCWGG 2 cut(s) 462, 929
BstNSI RCATGY 2 cut(s) 26, 445
BstSCI CCNGG 3 cut(s) 460, 790, 927
BstSNI TACGTA 1 cut(s) 328
BstV1I GCAGC 3 cut(s) 214, 541, 738
BstV2I GAAGAC 1 cut(s) 506
BstX2I RGATCY 3 cut(s) 9, 625, 692
BstXI CCANNNNNNTGG 1 cut(s) 928
BstYI RGATCY 3 cut(s) 9, 625, 692
BsuRI GGCC 2 cut(s) 156, 486
BtgI CCRYGG 1 cut(s) 608
BtgZI GCGATG 1 cut(s) 732
BtsCI GGATG 1 cut(s) 704
BtsI GCAGTG 1 cut(s) 828
BtsIMutI CAGTG 2 cut(s) 813, 828
BveI ACCTGC 1 cut(s) 343
Cac8I GCNNGC 2 cut(s) 207, 468
CfoI GCGC 1 cut(s) 813
Cfr13I GGNCC 1 cut(s) 71
Csp6I GTAC 2 cut(s) 115, 881
CviQI GTAC 2 cut(s) 115, 881
DdeI CTNAG 3 cut(s) 198, 618, 739
DpnI GATC 5 cut(s) 11, 564, 627, 694, 729
DpnII GATC 5 cut(s) 9, 562, 625, 692, 727
DrdI GACNNNNNNGTC 1 cut(s) 287
DseDI GACNNNNNNGTC 1 cut(s) 287
EaeI YGGCCR 1 cut(s) 484
Eam1104I CTCTTC 1 cut(s) 533
EarI CTCTTC 1 cut(s) 533
Ecl136II GAGCTC 1 cut(s) 544
Eco105I TACGTA 1 cut(s) 328
Eco130I CCWWGG 1 cut(s) 608
Eco24I GRGCYC 1 cut(s) 546
Eco47I GGWCC 1 cut(s) 71
Eco53kI GAGCTC 1 cut(s) 544
EcoICRI GAGCTC 1 cut(s) 544
EcoRII CCWGG 2 cut(s) 460, 927
EcoT14I CCWWGG 1 cut(s) 608
EcoT22I ATGCAT 1 cut(s) 643
EcoT38I GRGCYC 1 cut(s) 546
ErhI CCWWGG 1 cut(s) 608
FalI AAGNNNNNCTT 6 cut(s) 69, 101, 212, 244, 761, 793
FaqI GGGAC 1 cut(s) 883
FauI CCCGC 2 cut(s) 345, 459
FbaI TGATCA 1 cut(s) 562
Fnu4HI GCNGC 5 cut(s) 203, 487, 555, 633, 752
FokI GGATG 1 cut(s) 691
FriOI GRGCYC 1 cut(s) 546
Fsp4HI GCNGC 5 cut(s) 203, 487, 555, 633, 752
FspI TGCGCA 1 cut(s) 812
GlaI GCGC 1 cut(s) 812
GluI GCNGC 5 cut(s) 203, 487, 555, 633, 752
HaeIII GGCC 2 cut(s) 156, 486
HapII CCGG 5 cut(s) 104, 534, 660, 791, 896
HhaI GCGC 1 cut(s) 813
Hin6I GCGC 1 cut(s) 811
HinP1I GCGC 1 cut(s) 811
HincII GTYRAC 1 cut(s) 586
HindII GTYRAC 1 cut(s) 586
HindIII AAGCTT 1 cut(s) 44
HinfI GANTC 4 cut(s) 266, 435, 663, 871
HpaII CCGG 5 cut(s) 104, 534, 660, 791, 896
HphI GGTGA 3 cut(s) 517, 776, 910
Hpy166II GTNNAC 2 cut(s) 117, 586
Hpy188I TCNGA 3 cut(s) 199, 619, 709
Hpy188III TCNNGA 6 cut(s) 104, 534, 566, 660, 696, 896
Hpy8I GTNNAC 2 cut(s) 117, 586
HpyAV CCTTC 5 cut(s) 238, 295, 448, 763, 860
HpyCH4III ACNGT 3 cut(s) 161, 373, 505
HpyCH4IV ACGT 2 cut(s) 327, 700
HpyCH4V TGCA 7 cut(s) 28, 323, 391, 593, 641, 679, 863
HpyF10VI GCNNNNNNNGC 3 cut(s) 320, 638, 835
HpyF3I CTNAG 3 cut(s) 198, 618, 739
HpySE526I ACGT 2 cut(s) 327, 700
HspAI GCGC 1 cut(s) 811
Kpn2I TCCGGA 4 cut(s) 103, 533, 659, 895
Ksp22I TGATCA 1 cut(s) 562
Kzo9I GATC 5 cut(s) 9, 562, 625, 692, 727
LmnI GCTCC 5 cut(s) 311, 457, 549, 748, 898
Lsp1109I GCAGC 3 cut(s) 214, 541, 738
LweI GCATC 3 cut(s) 136, 388, 650
MaeII ACGT 2 cut(s) 327, 700
MalI GATC 5 cut(s) 11, 564, 627, 694, 729
MboI GATC 5 cut(s) 9, 562, 625, 692, 727
MboII GAAGA 3 cut(s) 511, 520, 635
MflI RGATCY 3 cut(s) 9, 625, 692
MhlI GDGCHC 1 cut(s) 546
MluCI AATT 5 cut(s) 60, 413, 681, 853, 890
MlyI GAGTC 2 cut(s) 657, 865
MnlI CCTC 8 cut(s) 29, 117, 248, 434, 609, 613, 703, 793
Mph1103I ATGCAT 1 cut(s) 643
MroI TCCGGA 4 cut(s) 103, 533, 659, 895
MroXI GAANNNNTTC 1 cut(s) 891
MseI TTAA 5 cut(s) 722, 782, 852, 906, 946
MslI CAYNNNNRTG 1 cut(s) 21
MspA1I CMGCKG 2 cut(s) 209, 352
MspI CCGG 5 cut(s) 104, 534, 660, 791, 896
MspR9I CCNGG 3 cut(s) 462, 792, 929
MvaI CCWGG 2 cut(s) 462, 929
MwoI GCNNNNNNNGC 3 cut(s) 320, 638, 835
NciI CCSGG 1 cut(s) 792
NcoI CCATGG 1 cut(s) 608
NdeII GATC 5 cut(s) 9, 562, 625, 692, 727
NlaIV GGNNCC 2 cut(s) 213, 459
NsbI TGCGCA 1 cut(s) 812
NsiI ATGCAT 1 cut(s) 643
NspI RCATGY 2 cut(s) 26, 445
PciI ACATGT 2 cut(s) 22, 441
PdmI GAANNNNTTC 1 cut(s) 891
PfeI GAWTC 2 cut(s) 266, 435
PfoI TCCNGGA 1 cut(s) 927
PkrI GCNGC 5 cut(s) 204, 488, 556, 634, 753
PleI GAGTC 2 cut(s) 657, 865
PpsI GAGTC 2 cut(s) 657, 865
Ppu21I YACGTR 1 cut(s) 328
PscI ACATGT 2 cut(s) 22, 441
PshBI ATTAAT 1 cut(s) 852
Psp124BI GAGCTC 1 cut(s) 546
Psp6I CCWGG 2 cut(s) 460, 927
PspGI CCWGG 2 cut(s) 460, 927
PspN4I GGNNCC 2 cut(s) 213, 459
PspPI GGNCC 1 cut(s) 71
PsuI RGATCY 3 cut(s) 9, 625, 692
RsaI GTAC 2 cut(s) 116, 882
RsaNI GTAC 2 cut(s) 115, 881
RseI CAYNNNNRTG 1 cut(s) 21
SacI GAGCTC 1 cut(s) 546
SaqAI TTAA 5 cut(s) 722, 782, 852, 906, 946
SatI GCNGC 5 cut(s) 203, 487, 555, 633, 752
Sau3AI GATC 5 cut(s) 9, 562, 625, 692, 727
Sau96I GGNCC 1 cut(s) 71
SchI GAGTC 2 cut(s) 657, 865
ScrFI CCNGG 3 cut(s) 462, 792, 929
SduI GDGCHC 1 cut(s) 546
SfaNI GCATC 3 cut(s) 136, 388, 650
SinI GGWCC 1 cut(s) 71
SmiMI CAYNNNNRTG 1 cut(s) 21
SnaBI TACGTA 1 cut(s) 328
Sse9I AATT 5 cut(s) 60, 413, 681, 853, 890
SsiI CCGC 6 cut(s) 69, 209, 352, 466, 487, 633
SstI GAGCTC 1 cut(s) 546
StyD4I CCNGG 3 cut(s) 460, 790, 927
StyI CCWWGG 1 cut(s) 608
TaaI ACNGT 3 cut(s) 161, 373, 505
TaiI ACGT 2 cut(s) 330, 703
TaqI TCGA 1 cut(s) 798
TasI AATT 5 cut(s) 60, 413, 681, 853, 890
TatI WGTACW 1 cut(s) 880
TauI GCSGC 2 cut(s) 489, 635
TfiI GAWTC 2 cut(s) 266, 435
Tru1I TTAA 5 cut(s) 722, 782, 852, 906, 946
Tru9I TTAA 5 cut(s) 722, 782, 852, 906, 946
TscAI CASTG 2 cut(s) 820, 828
TseI GCWGC 3 cut(s) 202, 554, 751
TspDTI ATGAA 5 cut(s) 38, 376, 407, 693, 847
TspGWI ACGGA 1 cut(s) 526
TspRI CASTG 2 cut(s) 820, 828
VpaK11BI GGWCC 1 cut(s) 71
VspI ATTAAT 1 cut(s) 852
XapI RAATTY 1 cut(s) 681
XceI RCATGY 2 cut(s) 26, 445
XmnI GAANNNNTTC 1 cut(s) 891
Zsp2I ATGCAT 1 cut(s) 643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.