MD17G1243900.v1.1

Bifunctional epoxide hydrolase 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0001763
Physical Location & Seq
Reverse (-)
29244016 .. 29246467
2452 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1243900.v1.1.491

Sequence Viewer

Length: 942 bp
ATGGAAAATATCGAGCACAAACATGTGGAAGTAAGAGGACTCAAGCTTCACGTAGCTGAAATCGGAAGCGGTCCAAAGGCTGTGGTTTTCCTTCATGGATTTCCGGAAATATGGTACACGTGGAGGCATCAACTGGTTGCTTTGGCAAACAAAGGCTACCGGGCAATAGCCATTGATTTCAGGGGCTATGGACTTTCCGAGCAGCCGGCTGACCCTGAAAAGGACACCTTCATTGACCTTGTCCACGATGTCGTTGCCCTTTTGGATTCTTTGGCCATTAACAAGGCTTTTCTTATTGGGAAAGATTTTGGAGCATTGCCTGCGTACTTAGTAGCTGCTCTCCACCCGGAGCGTGTATCTGGTGTCGTTACACTAGGTATTCCTTTCTCGCTACCAGGTCCCTCTGCTGTCCAAAATCATCTCCTTCCTGAAGTGTTCTACATATCAAGGTGGCAGGAACCAGGGAGGGCAGAAGCAGATTTTGGTCGCTTTGATGTGAAGACGGTGATTAGGAACATCTACATTCTCTTCTCTAGAAGTGAGATCCCAACAGCTGCTCCTGATCAAGAAATCATGGATTTGTTTGACCCTGCAACTCCTCTGCCGCCAGGGCTTTCGGAAGAAGATCTTGATGTCTACGCATCTTTGTATGAAAAATCTGGATTCCGTTATCCATTGCGAGTTCCATACAGAACCTTGGCCGTGGATTGCGGTATAACTGATCCCAAAGTCTCGGCTCCATCGCTGCTGATCGTGGGCGAGAAGGATTACGTCTTAAAATTTCCAGGGATGGAGGACTACATTCGCACTGGGGCGGTGAAGCATTTTGTGCCGGATCTGGACATTACATTCATCGAAGACGGGAGCCATTTTATGCACGAACAACTTGCAGAGAAAGTTAATCAGCTGATCCTCACATTCCTCGACAAGCATGCTATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000287 GO:0001676 GO:0003008 GO:0003013 GO:0003018 GO:0003674 GO:0003824 GO:0004301 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005782 GO:0005829 GO:0006082 GO:0006605 GO:0006625 GO:0006629 GO:0006631 GO:0006633 GO:0006690 GO:0006725 GO:0006793 GO:0006796 GO:0006805 GO:0006810 GO:0006873 GO:0006874 GO:0006875 GO:0006886 GO:0006950 GO:0006952 GO:0006954 GO:0006996 GO:0007031 GO:0008015 GO:0008104 GO:0008150 GO:0008152 GO:0008217 GO:0008610 GO:0009056 GO:0009058 GO:0009410 GO:0009636 GO:0009810 GO:0009893 GO:0009987 GO:0010468 GO:0010604 GO:0010628 GO:0015031 GO:0015643 GO:0015833 GO:0016043 GO:0016053 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0016801 GO:0016803 GO:0017144 GO:0018904 GO:0019216 GO:0019218 GO:0019222 GO:0019369 GO:0019373 GO:0019439 GO:0019725 GO:0019752 GO:0030003 GO:0030258 GO:0031907 GO:0031974 GO:0032501 GO:0032787 GO:0033036 GO:0033365 GO:0033559 GO:0034613 GO:0035150 GO:0035296 GO:0042221 GO:0042577 GO:0042578 GO:0042579 GO:0042592 GO:0042632 GO:0042759 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043574 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046272 GO:0046394 GO:0046483 GO:0046839 GO:0046872 GO:0046907 GO:0046983 GO:0048518 GO:0048878 GO:0050789 GO:0050801 GO:0050880 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0055088 GO:0055092 GO:0060255 GO:0062012 GO:0065007 GO:0065008 GO:0070013 GO:0070727 GO:0070887 GO:0071466 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072330 GO:0072503 GO:0072507 GO:0072593 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0090066 GO:0090181 GO:0097176 GO:0097746 GO:0097755 GO:0098771 GO:1900673 GO:1901360 GO:1901361 GO:1901568 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

314

Amino Acids

35.0

Weight (kDa)

5.27

Isoelectric Point (pI)

42.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 26 - 128 1.7e-11 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 27 - 154 2.6e-23 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 28 - 301 1.4e-17 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 636
AccIII TCCGGA 1 cut(s) 103
AciI CCGC 4 cut(s) 69, 605, 711, 815
AclWI GGATC 4 cut(s) 538, 716, 843, 904
AcoI YGGCCR 2 cut(s) 273, 699
AcsI RAATTY 1 cut(s) 779
AcuI CTGAAG 1 cut(s) 450
AcvI CACGTG 1 cut(s) 120
AfaI GTAC 2 cut(s) 116, 326
AfiI CCNNNNNNNGG 1 cut(s) 220
AflIII ACRYGT 2 cut(s) 22, 117
AjnI CCWGG 4 cut(s) 394, 460, 607, 784
AjuI GAANNNNNNNTTGG 2 cut(s) 465, 497
AluBI AGCT 5 cut(s) 46, 56, 335, 554, 907
AluI AGCT 5 cut(s) 46, 56, 335, 554, 907
Alw21I GWGCWC 1 cut(s) 18
Alw26I GTCTC 1 cut(s) 736
AlwI GGATC 4 cut(s) 538, 716, 843, 904
Aor13HI TCCGGA 1 cut(s) 103
AoxI GGCC 2 cut(s) 273, 699
ApeKI GCWGC 4 cut(s) 202, 335, 554, 745
ApoI RAATTY 1 cut(s) 779
AspS9I GGNCC 2 cut(s) 71, 398
AsuC2I CCSGG 2 cut(s) 161, 347
AsuHPI GGTGA 2 cut(s) 517, 829
AvaII GGWCC 2 cut(s) 71, 398
BalI TGGCCA 1 cut(s) 275
BbrPI CACGTG 1 cut(s) 120
BbsI GAAGAC 2 cut(s) 506, 864
Bbv12I GWGCWC 1 cut(s) 18
BbvI GCAGC 4 cut(s) 214, 322, 541, 732
BccI CCATC 2 cut(s) 748, 784
BceAI ACGGC 1 cut(s) 686
BcgI CGANNNNNNTGC 5 cut(s) 236, 270, 869, 903, 914
BciT130I CCWGG 4 cut(s) 396, 462, 609, 786
BclI TGATCA 1 cut(s) 562
BcnI CCSGG 2 cut(s) 161, 347
BcoDI GTCTC 1 cut(s) 736
BfaI CTAG 2 cut(s) 374, 534
BglI GCCNNNNNGGC 1 cut(s) 610
BglII AGATCT 1 cut(s) 625
BisI GCNGC 5 cut(s) 203, 336, 555, 605, 746
BlsI GCNGC 5 cut(s) 204, 337, 556, 606, 747
Bme1390I CCNGG 6 cut(s) 161, 347, 396, 462, 609, 786
Bme18I GGWCC 2 cut(s) 71, 398
BmgT120I GGNCC 2 cut(s) 71, 398
BmiI GGNNCC 4 cut(s) 400, 459, 738, 866
BmrFI CCNGG 6 cut(s) 161, 347, 396, 462, 609, 786
BmrI ACTGGG 1 cut(s) 819
BmsI GCATC 2 cut(s) 136, 650
BmuI ACTGGG 1 cut(s) 819
BpiI GAAGAC 2 cut(s) 506, 864
BpuEI CTTGAG 1 cut(s) 26
BpuMI CCSGG 2 cut(s) 161, 347
BsaAI YACGTR 2 cut(s) 52, 120
BsaJI CCNNGG 5 cut(s) 461, 608, 696, 702, 785
BsaWI WCCGGW 1 cut(s) 103
BsaXI ACNNNNNCTCC 2 cut(s) 541, 571
Bsc4I CCNNNNNNNGG 1 cut(s) 220
Bse118I RCCGGY 1 cut(s) 205
Bse1I ACTGG 2 cut(s) 138, 814
Bse3DI GCAATG 2 cut(s) 314, 674
BseAI TCCGGA 1 cut(s) 103
BseBI CCWGG 4 cut(s) 396, 462, 609, 786
BseDI CCNNGG 5 cut(s) 461, 608, 696, 702, 785
BseGI GGATG 1 cut(s) 795
BseLI CCNNNNNNNGG 1 cut(s) 220
BseMI GCAATG 2 cut(s) 314, 674
BseNI ACTGG 2 cut(s) 138, 814
BseRI GAGGAG 1 cut(s) 588
BseXI GCAGC 4 cut(s) 214, 322, 541, 732
BshFI GGCC 2 cut(s) 275, 701
BsiHKAI GWGCWC 1 cut(s) 18
BsiSI CCGG 5 cut(s) 104, 160, 206, 347, 833
BslFI GGGAC 1 cut(s) 384
BslI CCNNNNNNNGG 1 cut(s) 220
BsmAI GTCTC 1 cut(s) 736
BsmFI GGGAC 1 cut(s) 384
BsnI GGCC 2 cut(s) 275, 701
Bsp1286I GDGCHC 1 cut(s) 18
Bsp13I TCCGGA 1 cut(s) 103
Bsp143I GATC 7 cut(s) 543, 562, 625, 721, 750, 835, 909
BspACI CCGC 4 cut(s) 69, 605, 711, 815
BspANI GGCC 2 cut(s) 275, 701
BspEI TCCGGA 1 cut(s) 103
BspLI GGNNCC 4 cut(s) 400, 459, 738, 866
BspPI GGATC 4 cut(s) 538, 716, 843, 904
BsrDI GCAATG 2 cut(s) 314, 674
BsrFI RCCGGY 1 cut(s) 205
BsrI ACTGG 2 cut(s) 138, 814
BssAI RCCGGY 1 cut(s) 205
BssECI CCNNGG 5 cut(s) 461, 608, 696, 702, 785
BssMI GATC 7 cut(s) 543, 562, 625, 721, 750, 835, 909
BssT1I CCWWGG 1 cut(s) 696
Bst2UI CCWGG 4 cut(s) 396, 462, 609, 786
Bst4CI ACNGT 1 cut(s) 505
Bst6I CTCTTC 1 cut(s) 533
BstAPI GCANNNNNTGC 2 cut(s) 320, 829
BstBAI YACGTR 2 cut(s) 52, 120
BstC8I GCNNGC 3 cut(s) 207, 321, 933
BstDEI CTNAG 1 cut(s) 328
BstDSI CCRYGG 1 cut(s) 702
BstF5I GGATG 1 cut(s) 795
BstKTI GATC 7 cut(s) 546, 565, 628, 724, 753, 838, 912
BstMAI GTCTC 1 cut(s) 736
BstMBI GATC 7 cut(s) 543, 562, 625, 721, 750, 835, 909
BstMWI GCNNNNNNNGC 3 cut(s) 320, 610, 829
BstNI CCWGG 4 cut(s) 396, 462, 609, 786
BstNSI RCATGY 2 cut(s) 26, 935
BstSCI CCNGG 6 cut(s) 159, 345, 394, 460, 607, 784
BstV1I GCAGC 4 cut(s) 214, 322, 541, 732
BstV2I GAAGAC 2 cut(s) 506, 864
BstX2I RGATCY 3 cut(s) 543, 625, 835
BstYI RGATCY 3 cut(s) 543, 625, 835
BsuRI GGCC 2 cut(s) 275, 701
BtgI CCRYGG 1 cut(s) 702
BtgZI GCGATG 1 cut(s) 726
BtsCI GGATG 1 cut(s) 795
BtsIMutI CAGTG 1 cut(s) 807
Cac8I GCNNGC 3 cut(s) 207, 321, 933
Cfr10I RCCGGY 1 cut(s) 205
Cfr13I GGNCC 2 cut(s) 71, 398
CsiI ACCWGGT 1 cut(s) 394
Csp6I GTAC 2 cut(s) 115, 325
CspCI CAANNNNNGTGG 2 cut(s) 63, 98
CviAII CATG 4 cut(s) 23, 95, 574, 932
CviQI GTAC 2 cut(s) 115, 325
DdeI CTNAG 1 cut(s) 328
DpnI GATC 7 cut(s) 545, 564, 627, 723, 752, 837, 911
DpnII GATC 7 cut(s) 543, 562, 625, 721, 750, 835, 909
EaeI YGGCCR 2 cut(s) 273, 699
Eam1104I CTCTTC 1 cut(s) 533
EarI CTCTTC 1 cut(s) 533
Eco130I CCWWGG 1 cut(s) 696
Eco47I GGWCC 2 cut(s) 71, 398
Eco57I CTGAAG 1 cut(s) 450
Eco72I CACGTG 1 cut(s) 120
EcoO109I RGGNCCY 1 cut(s) 398
EcoRII CCWGG 4 cut(s) 394, 460, 607, 784
EcoT14I CCWWGG 1 cut(s) 696
ErhI CCWWGG 1 cut(s) 696
FaeI CATG 4 cut(s) 26, 98, 577, 935
FalI AAGNNNNNCTT 4 cut(s) 212, 244, 612, 644
FaqI GGGAC 1 cut(s) 384
FatI CATG 4 cut(s) 22, 94, 573, 931
FbaI TGATCA 1 cut(s) 562
FblI GTMKAC 1 cut(s) 636
Fnu4HI GCNGC 5 cut(s) 203, 336, 555, 605, 746
FokI GGATG 1 cut(s) 802
Fsp4HI GCNGC 5 cut(s) 203, 336, 555, 605, 746
FspBI CTAG 2 cut(s) 374, 534
GluI GCNGC 5 cut(s) 203, 336, 555, 605, 746
HaeIII GGCC 2 cut(s) 275, 701
HapII CCGG 5 cut(s) 104, 160, 206, 347, 833
Hin1II CATG 4 cut(s) 26, 98, 577, 935
HindIII AAGCTT 1 cut(s) 44
HinfI GANTC 3 cut(s) 39, 266, 663
HpaII CCGG 5 cut(s) 104, 160, 206, 347, 833
HphI GGTGA 2 cut(s) 517, 829
Hpy166II GTNNAC 3 cut(s) 117, 244, 637
Hpy188I TCNGA 4 cut(s) 65, 199, 619, 941
Hpy188III TCNNGA 8 cut(s) 104, 428, 534, 560, 566, 629, 660, 839
Hpy8I GTNNAC 3 cut(s) 117, 244, 637
HpyAV CCTTC 4 cut(s) 101, 238, 434, 757
HpyCH4III ACNGT 1 cut(s) 505
HpyCH4IV ACGT 3 cut(s) 51, 119, 771
HpyCH4V TGCA 3 cut(s) 593, 877, 890
HpyF10VI GCNNNNNNNGC 3 cut(s) 320, 610, 829
HpyF3I CTNAG 1 cut(s) 328
HpySE526I ACGT 3 cut(s) 51, 119, 771
Hsp92II CATG 4 cut(s) 26, 98, 577, 935
Kpn2I TCCGGA 1 cut(s) 103
KroI GCCGGC 1 cut(s) 205
KroNI GCCGGC 1 cut(s) 207
Ksp22I TGATCA 1 cut(s) 562
Kzo9I GATC 7 cut(s) 543, 562, 625, 721, 750, 835, 909
LmnI GCTCC 5 cut(s) 311, 349, 562, 742, 864
Lsp1109I GCAGC 4 cut(s) 214, 322, 541, 732
LweI GCATC 2 cut(s) 136, 650
MabI ACCWGGT 1 cut(s) 394
MaeI CTAG 2 cut(s) 374, 534
MaeII ACGT 3 cut(s) 51, 119, 771
MaeIII GTNAC 1 cut(s) 367
MalI GATC 7 cut(s) 545, 564, 627, 723, 752, 837, 911
MboI GATC 7 cut(s) 543, 562, 625, 721, 750, 835, 909
MboII GAAGA 5 cut(s) 511, 520, 632, 635, 869
MflI RGATCY 3 cut(s) 543, 625, 835
MhlI GDGCHC 1 cut(s) 18
MlsI TGGCCA 1 cut(s) 275
MluCI AATT 1 cut(s) 779
MluNI TGGCCA 1 cut(s) 275
MlyI GAGTC 1 cut(s) 33
MnlI CCTC 8 cut(s) 29, 117, 412, 459, 609, 787, 923, 932
Mox20I TGGCCA 1 cut(s) 275
MroI TCCGGA 1 cut(s) 103
MroNI GCCGGC 1 cut(s) 205
MscI TGGCCA 1 cut(s) 275
MseI TTAA 3 cut(s) 279, 776, 900
MslI CAYNNNNRTG 1 cut(s) 21
Msp20I TGGCCA 1 cut(s) 275
MspA1I CMGCKG 2 cut(s) 554, 907
MspI CCGG 5 cut(s) 104, 160, 206, 347, 833
MspR9I CCNGG 6 cut(s) 161, 347, 396, 462, 609, 786
MvaI CCWGG 4 cut(s) 396, 462, 609, 786
MwoI GCNNNNNNNGC 3 cut(s) 320, 610, 829
NaeI GCCGGC 1 cut(s) 207
NciI CCSGG 2 cut(s) 161, 347
NdeII GATC 7 cut(s) 543, 562, 625, 721, 750, 835, 909
NgoMIV GCCGGC 1 cut(s) 205
NlaIII CATG 4 cut(s) 26, 98, 577, 935
NlaIV GGNNCC 4 cut(s) 400, 459, 738, 866
NmeAIII GCCGAG 1 cut(s) 713
NspI RCATGY 2 cut(s) 26, 935
PaeI GCATGC 1 cut(s) 935
PciI ACATGT 1 cut(s) 22
PdiI GCCGGC 1 cut(s) 207
PfeI GAWTC 2 cut(s) 266, 663
PflFI GACNNNGTC 1 cut(s) 239
PkrI GCNGC 5 cut(s) 204, 337, 556, 606, 747
PleI GAGTC 1 cut(s) 33
PmaCI CACGTG 1 cut(s) 120
PmlI CACGTG 1 cut(s) 120
PpsI GAGTC 1 cut(s) 33
Ppu21I YACGTR 2 cut(s) 52, 120
PpuMI RGGWCCY 1 cut(s) 398
PscI ACATGT 1 cut(s) 22
Psp5II RGGWCCY 1 cut(s) 398
Psp6I CCWGG 4 cut(s) 394, 460, 607, 784
PspCI CACGTG 1 cut(s) 120
PspGI CCWGG 4 cut(s) 394, 460, 607, 784
PspN4I GGNNCC 4 cut(s) 400, 459, 738, 866
PspPI GGNCC 2 cut(s) 71, 398
PspPPI RGGWCCY 1 cut(s) 398
PsuI RGATCY 3 cut(s) 543, 625, 835
PsyI GACNNNGTC 1 cut(s) 239
PvuII CAGCTG 2 cut(s) 554, 907
RsaI GTAC 2 cut(s) 116, 326
RsaNI GTAC 2 cut(s) 115, 325
RseI CAYNNNNRTG 1 cut(s) 21
SaqAI TTAA 3 cut(s) 279, 776, 900
SatI GCNGC 5 cut(s) 203, 336, 555, 605, 746
Sau3AI GATC 7 cut(s) 543, 562, 625, 721, 750, 835, 909
Sau96I GGNCC 2 cut(s) 71, 398
SchI GAGTC 1 cut(s) 33
ScrFI CCNGG 6 cut(s) 161, 347, 396, 462, 609, 786
SduI GDGCHC 1 cut(s) 18
SexAI ACCWGGT 1 cut(s) 394
SfaNI GCATC 2 cut(s) 136, 650
SinI GGWCC 2 cut(s) 71, 398
SmiMI CAYNNNNRTG 1 cut(s) 21
SmlI CTYRAG 1 cut(s) 41
SmoI CTYRAG 1 cut(s) 41
SphI GCATGC 1 cut(s) 935
Sse9I AATT 1 cut(s) 779
SsiI CCGC 4 cut(s) 69, 605, 711, 815
SspMI CTAG 2 cut(s) 374, 534
StyD4I CCNGG 6 cut(s) 159, 345, 394, 460, 607, 784
StyI CCWWGG 1 cut(s) 696
TaaI ACNGT 1 cut(s) 505
TaiI ACGT 3 cut(s) 54, 122, 774
TaqI TCGA 3 cut(s) 12, 855, 924
TasI AATT 1 cut(s) 779
TauI GCSGC 1 cut(s) 607
TfiI GAWTC 2 cut(s) 266, 663
Tru1I TTAA 3 cut(s) 279, 776, 900
Tru9I TTAA 3 cut(s) 279, 776, 900
TscAI CASTG 1 cut(s) 814
TseI GCWGC 4 cut(s) 202, 335, 554, 745
TspDTI ATGAA 4 cut(s) 83, 220, 666, 841
TspGWI ACGGA 1 cut(s) 656
TspRI CASTG 1 cut(s) 814
Tth111I GACNNNGTC 1 cut(s) 239
VpaK11BI GGWCC 2 cut(s) 71, 398
XapI RAATTY 1 cut(s) 779
XbaI TCTAGA 1 cut(s) 533
XceI RCATGY 2 cut(s) 26, 935
XmiI GTMKAC 1 cut(s) 636
XspI CTAG 2 cut(s) 374, 534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.