Rmu_sc0011122.1_g000006

Bifunctional epoxide hydrolase 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0011122.1
OG0001763
Physical Location & Seq
Forward (+)
30308 .. 32233
1926 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0011122.1_g000006.1.cds

Sequence Viewer

Length: 717 bp
atggaaaagattgagcataagtatgtggaggtagaaggactaaagcttcatgtggctgaaattggaagtggtccaaaggtggtggttttcttgcatggatttccagaaatatggtactcatggaggcaccaaatgattgctgtggccaacaatggctatcgggcaatttcctatgattgcaggggatatggactttctgagctgccagctgagcctgaaaaggcaactttcaaggacctcgttgacgaggttgttgtagttttggatgctttggtaattgacaaggagccagggagggcggaagcagattttgacctctttgatgtcaagtcggtggatcaagaaatcatggatttgtttgatccagctattcctctaccgccagggttctcagaggaagatctttcagtctacgcatctctgtatgagaagtctggattccgttttcctttgagagttccatataggagtttaacagtggattgtggttatactgatccaaaagtctcagctccaacactggttatccagggtgagaaagactacctcttaaattttcccgggactgaggacaacatacgatctggggtaatgaaacatgttgtgcctgatttggacattaccttcattgcagaagggggtcactttgtgcaagaacaacttccagagaaaattaatgagctaatcctcactttcctcagcaaacatggtatctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000287 GO:0001676 GO:0003008 GO:0003013 GO:0003018 GO:0003674 GO:0003824 GO:0004301 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005782 GO:0005829 GO:0006082 GO:0006605 GO:0006625 GO:0006629 GO:0006631 GO:0006633 GO:0006690 GO:0006725 GO:0006793 GO:0006796 GO:0006805 GO:0006810 GO:0006873 GO:0006874 GO:0006875 GO:0006886 GO:0006950 GO:0006952 GO:0006954 GO:0006996 GO:0007031 GO:0008015 GO:0008104 GO:0008150 GO:0008152 GO:0008217 GO:0008610 GO:0009056 GO:0009058 GO:0009410 GO:0009636 GO:0009810 GO:0009893 GO:0009987 GO:0010468 GO:0010604 GO:0010628 GO:0015031 GO:0015643 GO:0015833 GO:0016043 GO:0016053 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0016801 GO:0016803 GO:0017144 GO:0018904 GO:0019216 GO:0019218 GO:0019222 GO:0019369 GO:0019373 GO:0019439 GO:0019725 GO:0019752 GO:0030003 GO:0030258 GO:0031907 GO:0031974 GO:0032501 GO:0032787 GO:0033036 GO:0033365 GO:0033559 GO:0034613 GO:0035150 GO:0035296 GO:0042221 GO:0042577 GO:0042578 GO:0042579 GO:0042592 GO:0042632 GO:0042759 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043574 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046272 GO:0046394 GO:0046483 GO:0046839 GO:0046872 GO:0046907 GO:0046983 GO:0048518 GO:0048878 GO:0050789 GO:0050801 GO:0050880 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0055088 GO:0055092 GO:0060255 GO:0062012 GO:0065007 GO:0065008 GO:0070013 GO:0070727 GO:0070887 GO:0071466 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072330 GO:0072503 GO:0072507 GO:0072593 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0090066 GO:0090181 GO:0097176 GO:0097746 GO:0097755 GO:0098771 GO:1900673 GO:1901360 GO:1901361 GO:1901568 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

238

Amino Acids

26.76

Weight (kDa)

4.7

Isoelectric Point (pI)

35.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 126
AccI GTMKAC 1 cut(s) 411
AciI CCGC 2 cut(s) 299, 380
AclWI GGATC 3 cut(s) 345, 356, 491
AcoI YGGCCR 1 cut(s) 144
AcsI RAATTY 1 cut(s) 553
AdeI CACNNNGTG 1 cut(s) 649
AfaI GTAC 1 cut(s) 116
AflIII ACRYGT 1 cut(s) 598
AgsI TTSAA 1 cut(s) 232
AjnI CCWGG 3 cut(s) 289, 382, 528
AluBI AGCT 6 cut(s) 46, 202, 209, 368, 512, 682
AluI AGCT 6 cut(s) 46, 202, 209, 368, 512, 682
Alw26I GTCTC 1 cut(s) 511
AlwI GGATC 3 cut(s) 345, 356, 491
Ama87I CYCGRG 1 cut(s) 560
AoxI GGCC 1 cut(s) 144
ApeKI GCWGC 1 cut(s) 202
ApoI RAATTY 1 cut(s) 553
AseI ATTAAT 1 cut(s) 675
Asp700I GAANNNNTTC 1 cut(s) 660
AspS9I GGNCC 2 cut(s) 71, 235
AsuC2I CCSGG 2 cut(s) 561, 562
AsuHPI GGTGA 1 cut(s) 545
AvaI CYCGRG 1 cut(s) 560
AvaII GGWCC 2 cut(s) 71, 235
BalI TGGCCA 1 cut(s) 146
BanI GGYRCC 1 cut(s) 126
BbvCI CCTCAGC 1 cut(s) 698
BbvI GCAGC 1 cut(s) 189
BciT130I CCWGG 3 cut(s) 291, 384, 530
BcnI CCSGG 2 cut(s) 561, 562
BcoDI GTCTC 1 cut(s) 511
BglII AGATCT 1 cut(s) 400
BisI GCNGC 1 cut(s) 203
BlpI GCTNAGC 1 cut(s) 210
BlsI GCNGC 1 cut(s) 204
Bme1390I CCNGG 5 cut(s) 291, 384, 530, 561, 562
Bme18I GGWCC 2 cut(s) 71, 235
BmeT110I CYCGRG 1 cut(s) 560
BmgT120I GGNCC 2 cut(s) 71, 235
BmiI GGNNCC 2 cut(s) 128, 288
BmrFI CCNGG 5 cut(s) 291, 384, 530, 561, 562
BmsI GCATC 2 cut(s) 256, 425
Bpu10I CCTNAGC 1 cut(s) 698
Bpu1102I GCTNAGC 1 cut(s) 210
BpuMI CCSGG 2 cut(s) 561, 562
BsaJI CCNNGG 4 cut(s) 290, 383, 529, 560
Bse1I ACTGG 1 cut(s) 525
Bse3DI GCAATG 1 cut(s) 627
BseBI CCWGG 3 cut(s) 291, 384, 530
BseDI CCNNGG 4 cut(s) 290, 383, 529, 560
BseGI GGATG 1 cut(s) 271
BseMI GCAATG 1 cut(s) 627
BseMII CTCAG 6 cut(s) 189, 201, 405, 522, 558, 712
BseNI ACTGG 1 cut(s) 525
BseXI GCAGC 1 cut(s) 189
BshFI GGCC 1 cut(s) 146
BshNI GGYRCC 1 cut(s) 126
BsiHKCI CYCGRG 1 cut(s) 560
BsiSI CCGG 1 cut(s) 561
BslFI GGGAC 1 cut(s) 577
BsmAI GTCTC 1 cut(s) 511
BsmFI GGGAC 1 cut(s) 577
BsnI GGCC 1 cut(s) 146
BsoBI CYCGRG 1 cut(s) 560
Bsp143I GATC 5 cut(s) 337, 361, 400, 496, 581
Bsp1720I GCTNAGC 1 cut(s) 210
BspACI CCGC 2 cut(s) 299, 380
BspANI GGCC 1 cut(s) 146
BspCNI CTCAG 6 cut(s) 190, 202, 404, 521, 559, 711
BspLI GGNNCC 2 cut(s) 128, 288
BspPI GGATC 3 cut(s) 345, 356, 491
BspT107I GGYRCC 1 cut(s) 126
BsrDI GCAATG 1 cut(s) 627
BsrI ACTGG 1 cut(s) 525
BssECI CCNNGG 4 cut(s) 290, 383, 529, 560
BssMI GATC 5 cut(s) 337, 361, 400, 496, 581
Bst2UI CCWGG 3 cut(s) 291, 384, 530
Bst4CI ACNGT 1 cut(s) 478
BstC8I GCNNGC 1 cut(s) 207
BstDEI CTNAG 6 cut(s) 198, 210, 391, 508, 567, 698
BstF5I GGATG 1 cut(s) 271
BstKTI GATC 5 cut(s) 340, 364, 403, 499, 584
BstMAI GTCTC 1 cut(s) 511
BstMBI GATC 5 cut(s) 337, 361, 400, 496, 581
BstMWI GCNNNNNNNGC 1 cut(s) 211
BstNI CCWGG 3 cut(s) 291, 384, 530
BstNSI RCATGY 1 cut(s) 602
BstSCI CCNGG 5 cut(s) 289, 382, 528, 559, 560
BstV1I GCAGC 1 cut(s) 189
BstX2I RGATCY 1 cut(s) 400
BstXI CCANNNNNNTGG 1 cut(s) 111
BstYI RGATCY 1 cut(s) 400
BsuRI GGCC 1 cut(s) 146
BtsCI GGATG 1 cut(s) 271
BtsIMutI CAGTG 2 cut(s) 483, 518
Cac8I GCNNGC 1 cut(s) 207
Cfr13I GGNCC 2 cut(s) 71, 235
Cfr9I CCCGGG 1 cut(s) 560
Csp6I GTAC 1 cut(s) 115
CspCI CAANNNNNGTGG 2 cut(s) 63, 98
CviAII CATG 6 cut(s) 50, 95, 120, 349, 599, 707
CviQI GTAC 1 cut(s) 115
DdeI CTNAG 6 cut(s) 198, 210, 391, 508, 567, 698
DpnI GATC 5 cut(s) 339, 363, 402, 498, 583
DpnII GATC 5 cut(s) 337, 361, 400, 496, 581
DraIII CACNNNGTG 1 cut(s) 649
EaeI YGGCCR 1 cut(s) 144
EciI GGCGGA 1 cut(s) 314
Eco47I GGWCC 2 cut(s) 71, 235
Eco88I CYCGRG 1 cut(s) 560
EcoO109I RGGNCCY 1 cut(s) 235
EcoRII CCWGG 3 cut(s) 289, 382, 528
FaeI CATG 6 cut(s) 53, 98, 123, 352, 602, 710
FalI AAGNNNNNCTT 2 cut(s) 645, 677
FaqI GGGAC 1 cut(s) 577
FatI CATG 6 cut(s) 49, 94, 119, 348, 598, 706
FblI GTMKAC 1 cut(s) 411
Fnu4HI GCNGC 1 cut(s) 203
FokI GGATG 1 cut(s) 278
Fsp4HI GCNGC 1 cut(s) 203
GluI GCNGC 1 cut(s) 203
HaeIII GGCC 1 cut(s) 146
HapII CCGG 1 cut(s) 561
Hin1II CATG 6 cut(s) 53, 98, 123, 352, 602, 710
HincII GTYRAC 1 cut(s) 244
HindII GTYRAC 1 cut(s) 244
HindIII AAGCTT 1 cut(s) 44
HinfI GANTC 1 cut(s) 438
HpaII CCGG 1 cut(s) 561
HphI GGTGA 1 cut(s) 545
Hpy166II GTNNAC 2 cut(s) 244, 412
Hpy188I TCNGA 3 cut(s) 199, 394, 716
Hpy188III TCNNGA 4 cut(s) 104, 341, 435, 665
Hpy8I GTNNAC 2 cut(s) 244, 412
HpyAV CCTTC 3 cut(s) 29, 629, 634
HpyCH4III ACNGT 1 cut(s) 478
HpyCH4V TGCA 4 cut(s) 94, 180, 632, 652
HpyF10VI GCNNNNNNNGC 1 cut(s) 211
HpyF3I CTNAG 6 cut(s) 198, 210, 391, 508, 567, 698
Hsp92II CATG 6 cut(s) 53, 98, 123, 352, 602, 710
Kzo9I GATC 5 cut(s) 337, 361, 400, 496, 581
LmnI GCTCC 2 cut(s) 286, 517
Lsp1109I GCAGC 1 cut(s) 189
LweI GCATC 2 cut(s) 256, 425
MaeIII GTNAC 1 cut(s) 641
MalI GATC 5 cut(s) 339, 363, 402, 498, 583
MboI GATC 5 cut(s) 337, 361, 400, 496, 581
MboII GAAGA 1 cut(s) 410
MflI RGATCY 1 cut(s) 400
MlsI TGGCCA 1 cut(s) 146
MluCI AATT 5 cut(s) 60, 165, 276, 553, 672
MluNI TGGCCA 1 cut(s) 146
MmeI TCCRAC 1 cut(s) 539
Mox20I TGGCCA 1 cut(s) 146
MroXI GAANNNNTTC 1 cut(s) 660
MscI TGGCCA 1 cut(s) 146
MseI TTAA 3 cut(s) 473, 551, 675
MslI CAYNNNNRTG 1 cut(s) 21
Msp20I TGGCCA 1 cut(s) 146
MspA1I CMGCKG 1 cut(s) 209
MspI CCGG 1 cut(s) 561
MspR9I CCNGG 5 cut(s) 291, 384, 530, 561, 562
MvaI CCWGG 3 cut(s) 291, 384, 530
MwoI GCNNNNNNNGC 1 cut(s) 211
NciI CCSGG 2 cut(s) 561, 562
NdeII GATC 5 cut(s) 337, 361, 400, 496, 581
NlaIII CATG 6 cut(s) 53, 98, 123, 352, 602, 710
NlaIV GGNNCC 2 cut(s) 128, 288
NmuCI GTSAC 1 cut(s) 641
NspI RCATGY 1 cut(s) 602
PciI ACATGT 1 cut(s) 598
PdmI GAANNNNTTC 1 cut(s) 660
PfeI GAWTC 1 cut(s) 438
PkrI GCNGC 1 cut(s) 204
PpuMI RGGWCCY 1 cut(s) 235
PscI ACATGT 1 cut(s) 598
PshBI ATTAAT 1 cut(s) 675
Psp5II RGGWCCY 1 cut(s) 235
Psp6I CCWGG 3 cut(s) 289, 382, 528
PspGI CCWGG 3 cut(s) 289, 382, 528
PspN4I GGNNCC 2 cut(s) 128, 288
PspPI GGNCC 2 cut(s) 71, 235
PspPPI RGGWCCY 1 cut(s) 235
PsuI RGATCY 1 cut(s) 400
PvuII CAGCTG 1 cut(s) 209
RsaI GTAC 1 cut(s) 116
RsaNI GTAC 1 cut(s) 115
RseI CAYNNNNRTG 1 cut(s) 21
SaqAI TTAA 3 cut(s) 473, 551, 675
SatI GCNGC 1 cut(s) 203
Sau3AI GATC 5 cut(s) 337, 361, 400, 496, 581
Sau96I GGNCC 2 cut(s) 71, 235
ScrFI CCNGG 5 cut(s) 291, 384, 530, 561, 562
SfaNI GCATC 2 cut(s) 256, 425
SinI GGWCC 2 cut(s) 71, 235
SmaI CCCGGG 1 cut(s) 562
SmiMI CAYNNNNRTG 1 cut(s) 21
Sse9I AATT 5 cut(s) 60, 165, 276, 553, 672
SsiI CCGC 2 cut(s) 299, 380
StyD4I CCNGG 5 cut(s) 289, 382, 528, 559, 560
TaaI ACNGT 1 cut(s) 478
TasI AATT 5 cut(s) 60, 165, 276, 553, 672
TfiI GAWTC 1 cut(s) 438
Tru1I TTAA 3 cut(s) 473, 551, 675
Tru9I TTAA 3 cut(s) 473, 551, 675
TscAI CASTG 2 cut(s) 483, 525
TseFI GTSAC 1 cut(s) 641
TseI GCWGC 1 cut(s) 202
Tsp45I GTSAC 1 cut(s) 641
TspDTI ATGAA 3 cut(s) 38, 608, 616
TspGWI ACGGA 1 cut(s) 431
TspMI CCCGGG 1 cut(s) 560
TspRI CASTG 2 cut(s) 483, 525
VpaK11BI GGWCC 2 cut(s) 71, 235
VspI ATTAAT 1 cut(s) 675
XapI RAATTY 1 cut(s) 553
XceI RCATGY 1 cut(s) 602
XmaI CCCGGG 1 cut(s) 560
XmiI GTMKAC 1 cut(s) 411
XmnI GAANNNNTTC 1 cut(s) 660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.