pycom09g16790

Bifunctional epoxide hydrolase 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0001763
Physical Location & Seq
Reverse (-)
17167018 .. 17168179
1162 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g16790.2

Sequence Viewer

Length: 819 bp
ATGGTTGCTGTTGCAGACAAAGGCCACCGCGCAATAGCCATTGATTTCAGGGGCCTTGGACTATCCGAGCAGCCAGCTGAATCCGAAAAGGCAATCTTCAAAGACCTTGTTGACGATGTCGTTGGCCTTCTGGATTCTTTAAGCATTAACAAGGTTGTTTTTGTTGGGAAGGATTTTGGAGCTCTGCCTGCATACCTAATACCTGCTCTCCACCCAGAGTGCATATCCGGCATGATAACAGTAGGTGTTCCTTTCCTCAACCCGGCTAACTCTCCTGTCCGATTTGATCTCTTACCCAAAGGCTTCTACATGTTAAGATGGAAGAAGCCGGGGCGGGCAGAAGCAGATATTAGCCGCTTTGATGTCAAGACAGTGATACGAAACATCTACATTCTCTTCTCCGGAAGTGAGCTCCAAGTAGCTGCTCCTGATCAAGAAATCATGGACTTGGTTGACCCTGCCACTCCTCTACCACCATGGTTTTCGGAGGATGATCTTGCAGTCTATGCATCCTTATATGAGAAATCTGGATTCCGCTATGCCATCCAAATTACATACCAATCCACAACTAAGGTTGTTGATTACGGATTAGGCAGGGATCCAAAAGTGCCCGCTCCATCACTGCTTATCATGGGTGAGAAGGACTACATCTTAAAATTTCCAGGGATGGAGGACTACATACGGTCTGGGGCAGTGAAGCATTTTGTACCCGATTTGGACATTGCATGTATTGCAGAAGGGAGTCACTTTGTGCACGAACAATTTCCTGATCAAGTTAATCAGTTCATGATCAGTTTCCTTGAGAAACATGGTATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000287 GO:0001676 GO:0003008 GO:0003013 GO:0003018 GO:0003674 GO:0003824 GO:0004301 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005782 GO:0005829 GO:0006082 GO:0006605 GO:0006625 GO:0006629 GO:0006631 GO:0006633 GO:0006690 GO:0006725 GO:0006793 GO:0006796 GO:0006805 GO:0006810 GO:0006873 GO:0006874 GO:0006875 GO:0006886 GO:0006950 GO:0006952 GO:0006954 GO:0006996 GO:0007031 GO:0008015 GO:0008104 GO:0008150 GO:0008152 GO:0008217 GO:0008610 GO:0009056 GO:0009058 GO:0009410 GO:0009636 GO:0009810 GO:0009893 GO:0009987 GO:0010468 GO:0010604 GO:0010628 GO:0015031 GO:0015643 GO:0015833 GO:0016043 GO:0016053 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0016801 GO:0016803 GO:0017144 GO:0018904 GO:0019216 GO:0019218 GO:0019222 GO:0019369 GO:0019373 GO:0019439 GO:0019725 GO:0019752 GO:0030003 GO:0030258 GO:0031907 GO:0031974 GO:0032501 GO:0032787 GO:0033036 GO:0033365 GO:0033559 GO:0034613 GO:0035150 GO:0035296 GO:0042221 GO:0042577 GO:0042578 GO:0042579 GO:0042592 GO:0042632 GO:0042759 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043574 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046272 GO:0046394 GO:0046483 GO:0046839 GO:0046872 GO:0046907 GO:0046983 GO:0048518 GO:0048878 GO:0050789 GO:0050801 GO:0050880 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0055088 GO:0055092 GO:0060255 GO:0062012 GO:0065007 GO:0065008 GO:0070013 GO:0070727 GO:0070887 GO:0071466 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072330 GO:0072503 GO:0072507 GO:0072593 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0090066 GO:0090181 GO:0097176 GO:0097746 GO:0097755 GO:0098771 GO:1900673 GO:1901360 GO:1901361 GO:1901568 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

273

Amino Acids

30.18

Weight (kDa)

5.07

Isoelectric Point (pI)

42.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 211
AccBSI CCGCTC 1 cut(s) 614
AccII CGCG 1 cut(s) 30
AccIII TCCGGA 1 cut(s) 401
AciI CCGC 5 cut(s) 28, 334, 355, 535, 612
AclWI GGATC 2 cut(s) 593, 606
AcsI RAATTY 1 cut(s) 656
AdeI CACNNNGTG 1 cut(s) 751
AfaI GTAC 1 cut(s) 708
AfiI CCNNNNNNNGG 1 cut(s) 262
AflIII ACRYGT 1 cut(s) 309
AgsI TTSAA 1 cut(s) 100
AjnI CCWGG 1 cut(s) 661
AluBI AGCT 4 cut(s) 77, 182, 412, 422
AluI AGCT 4 cut(s) 77, 182, 412, 422
Alw21I GWGCWC 3 cut(s) 184, 414, 756
Alw44I GTGCAC 1 cut(s) 752
AlwI GGATC 2 cut(s) 593, 606
Aor13HI TCCGGA 1 cut(s) 401
AoxI GGCC 3 cut(s) 22, 52, 124
ApaLI GTGCAC 1 cut(s) 752
ApeKI GCWGC 2 cut(s) 70, 422
ApoI RAATTY 1 cut(s) 656
Asp700I GAANNNNTTC 1 cut(s) 762
AspLEI GCGC 1 cut(s) 32
AspS9I GGNCC 1 cut(s) 52
AsuC2I CCSGG 2 cut(s) 263, 330
AsuHPI GGTGA 1 cut(s) 647
BaeGI GKGCMC 2 cut(s) 612, 756
BamHI GGATCC 1 cut(s) 598
BanII GRGCYC 2 cut(s) 184, 414
Bbv12I GWGCWC 3 cut(s) 184, 414, 756
BbvI GCAGC 2 cut(s) 82, 409
BccI CCATC 4 cut(s) 312, 551, 625, 661
BciT130I CCWGG 1 cut(s) 663
BclI TGATCA 3 cut(s) 430, 769, 789
BcnI CCSGG 2 cut(s) 263, 330
BfuAI ACCTGC 1 cut(s) 211
BisI GCNGC 3 cut(s) 71, 355, 423
BlsI GCNGC 3 cut(s) 72, 356, 424
Bme1390I CCNGG 3 cut(s) 263, 330, 663
BmgT120I GGNCC 1 cut(s) 52
BmiI GGNNCC 2 cut(s) 53, 600
BmrFI CCNGG 3 cut(s) 263, 330, 663
BmsI GCATC 1 cut(s) 518
BpuMI CCSGG 2 cut(s) 263, 330
BsaJI CCNNGG 4 cut(s) 55, 329, 476, 662
BsaWI WCCGGW 1 cut(s) 401
BsaXI ACNNNNNCTCC 2 cut(s) 192, 222
Bsc4I CCNNNNNNNGG 1 cut(s) 262
Bse3DI GCAATG 1 cut(s) 720
BseAI TCCGGA 1 cut(s) 401
BseBI CCWGG 1 cut(s) 663
BseDI CCNNGG 4 cut(s) 55, 329, 476, 662
BseGI GGATG 4 cut(s) 496, 509, 543, 672
BseLI CCNNNNNNNGG 1 cut(s) 262
BseMI GCAATG 1 cut(s) 720
BseRI GAGGAG 1 cut(s) 456
BseSI GKGCMC 2 cut(s) 612, 756
BseXI GCAGC 2 cut(s) 82, 409
Bsh1236I CGCG 1 cut(s) 30
BshFI GGCC 3 cut(s) 24, 54, 126
BsiHKAI GWGCWC 3 cut(s) 184, 414, 756
BsiSI CCGG 4 cut(s) 228, 263, 329, 402
BslI CCNNNNNNNGG 1 cut(s) 262
BsnI GGCC 3 cut(s) 24, 54, 126
Bsp1286I GDGCHC 4 cut(s) 184, 414, 612, 756
Bsp13I TCCGGA 1 cut(s) 401
Bsp143I GATC 6 cut(s) 286, 430, 493, 598, 769, 789
Bsp19I CCATGG 1 cut(s) 476
BspACI CCGC 5 cut(s) 28, 334, 355, 535, 612
BspANI GGCC 3 cut(s) 24, 54, 126
BspEI TCCGGA 1 cut(s) 401
BspFNI CGCG 1 cut(s) 30
BspHI TCATGA 1 cut(s) 786
BspLI GGNNCC 2 cut(s) 53, 600
BspMI ACCTGC 1 cut(s) 211
BspPI GGATC 2 cut(s) 593, 606
BsrBI CCGCTC 1 cut(s) 614
BsrDI GCAATG 1 cut(s) 720
BssECI CCNNGG 4 cut(s) 55, 329, 476, 662
BssMI GATC 6 cut(s) 286, 430, 493, 598, 769, 789
BssT1I CCWWGG 2 cut(s) 55, 476
Bst2UI CCWGG 1 cut(s) 663
Bst4CI ACNGT 3 cut(s) 241, 373, 684
Bst6I CTCTTC 1 cut(s) 401
BstAPI GCANNNNNTGC 2 cut(s) 506, 731
BstC8I GCNNGC 4 cut(s) 75, 189, 336, 612
BstDEI CTNAG 1 cut(s) 570
BstDSI CCRYGG 1 cut(s) 476
BstF5I GGATG 4 cut(s) 496, 509, 543, 672
BstFNI CGCG 1 cut(s) 30
BstHHI GCGC 1 cut(s) 32
BstKTI GATC 6 cut(s) 289, 433, 496, 601, 772, 792
BstMBI GATC 6 cut(s) 286, 430, 493, 598, 769, 789
BstMWI GCNNNNNNNGC 4 cut(s) 188, 228, 506, 731
BstNI CCWGG 1 cut(s) 663
BstNSI RCATGY 2 cut(s) 313, 729
BstSCI CCNGG 3 cut(s) 261, 328, 661
BstSLI GKGCMC 2 cut(s) 612, 756
BstUI CGCG 1 cut(s) 30
BstV1I GCAGC 2 cut(s) 82, 409
BstX2I RGATCY 1 cut(s) 598
BstYI RGATCY 1 cut(s) 598
BsuRI GGCC 3 cut(s) 24, 54, 126
BtgI CCRYGG 1 cut(s) 476
BtsCI GGATG 4 cut(s) 496, 509, 543, 672
BtsI GCAGTG 2 cut(s) 620, 699
BtsIMutI CAGTG 3 cut(s) 378, 620, 699
BveI ACCTGC 1 cut(s) 211
Cac8I GCNNGC 4 cut(s) 75, 189, 336, 612
CciI TCATGA 1 cut(s) 786
CfoI GCGC 1 cut(s) 32
Cfr13I GGNCC 1 cut(s) 52
Csp6I GTAC 1 cut(s) 707
CviAII CATG 8 cut(s) 232, 310, 442, 477, 631, 726, 787, 809
CviQI GTAC 1 cut(s) 707
DdeI CTNAG 1 cut(s) 570
DpnI GATC 6 cut(s) 288, 432, 495, 600, 771, 791
DpnII GATC 6 cut(s) 286, 430, 493, 598, 769, 789
DraIII CACNNNGTG 1 cut(s) 751
Eam1104I CTCTTC 1 cut(s) 401
EarI CTCTTC 1 cut(s) 401
Ecl136II GAGCTC 2 cut(s) 182, 412
Eco130I CCWWGG 2 cut(s) 55, 476
Eco24I GRGCYC 2 cut(s) 184, 414
Eco53kI GAGCTC 2 cut(s) 182, 412
EcoICRI GAGCTC 2 cut(s) 182, 412
EcoO109I RGGNCCY 1 cut(s) 52
EcoRII CCWGG 1 cut(s) 661
EcoT14I CCWWGG 2 cut(s) 55, 476
EcoT22I ATGCAT 1 cut(s) 511
EcoT38I GRGCYC 2 cut(s) 184, 414
ErhI CCWWGG 2 cut(s) 55, 476
FaeI CATG 8 cut(s) 235, 313, 445, 480, 634, 729, 790, 812
FalI AAGNNNNNCTT 2 cut(s) 80, 112
FatI CATG 8 cut(s) 231, 309, 441, 476, 630, 725, 786, 808
FauI CCCGC 2 cut(s) 327, 619
FbaI TGATCA 3 cut(s) 430, 769, 789
Fnu4HI GCNGC 3 cut(s) 71, 355, 423
FokI GGATG 4 cut(s) 496, 503, 530, 679
FriOI GRGCYC 2 cut(s) 184, 414
Fsp4HI GCNGC 3 cut(s) 71, 355, 423
GlaI GCGC 1 cut(s) 31
GluI GCNGC 3 cut(s) 71, 355, 423
HaeIII GGCC 3 cut(s) 24, 54, 126
HapII CCGG 4 cut(s) 228, 263, 329, 402
HhaI GCGC 1 cut(s) 32
Hin1II CATG 8 cut(s) 235, 313, 445, 480, 634, 729, 790, 812
Hin6I GCGC 1 cut(s) 30
HinP1I GCGC 1 cut(s) 30
HincII GTYRAC 2 cut(s) 112, 454
HindII GTYRAC 2 cut(s) 112, 454
HinfI GANTC 4 cut(s) 80, 134, 531, 742
HpaII CCGG 4 cut(s) 228, 263, 329, 402
HphI GGTGA 1 cut(s) 647
Hpy166II GTNNAC 3 cut(s) 112, 454, 754
Hpy188I TCNGA 4 cut(s) 67, 85, 281, 487
Hpy188III TCNNGA 8 cut(s) 131, 367, 402, 428, 434, 528, 767, 787
Hpy8I GTNNAC 3 cut(s) 112, 454, 754
HpyAV CCTTC 4 cut(s) 137, 163, 634, 731
HpyCH4III ACNGT 3 cut(s) 241, 373, 684
HpyCH4V TGCA 8 cut(s) 14, 191, 222, 500, 509, 725, 734, 754
HpyF10VI GCNNNNNNNGC 4 cut(s) 188, 228, 506, 731
HpyF3I CTNAG 1 cut(s) 570
Hsp92II CATG 8 cut(s) 235, 313, 445, 480, 634, 729, 790, 812
HspAI GCGC 1 cut(s) 30
Kpn2I TCCGGA 1 cut(s) 401
Ksp22I TGATCA 3 cut(s) 430, 769, 789
Kzo9I GATC 6 cut(s) 286, 430, 493, 598, 769, 789
LmnI GCTCC 4 cut(s) 179, 417, 430, 619
Lsp1109I GCAGC 2 cut(s) 82, 409
LweI GCATC 1 cut(s) 518
MaeIII GTNAC 1 cut(s) 743
MalI GATC 6 cut(s) 288, 432, 495, 600, 771, 791
MbiI CCGCTC 1 cut(s) 614
MboI GATC 6 cut(s) 286, 430, 493, 598, 769, 789
MboII GAAGA 3 cut(s) 88, 334, 388
MflI RGATCY 1 cut(s) 598
MhlI GDGCHC 4 cut(s) 184, 414, 612, 756
MluCI AATT 3 cut(s) 549, 656, 761
MlyI GAGTC 1 cut(s) 751
MnlI CCTC 4 cut(s) 266, 477, 481, 664
Mph1103I ATGCAT 1 cut(s) 511
MroI TCCGGA 1 cut(s) 401
MroXI GAANNNNTTC 1 cut(s) 762
MseI TTAA 5 cut(s) 140, 147, 314, 653, 777
MspA1I CMGCKG 1 cut(s) 77
MspI CCGG 4 cut(s) 228, 263, 329, 402
MspR9I CCNGG 3 cut(s) 263, 330, 663
MvaI CCWGG 1 cut(s) 663
MvnI CGCG 1 cut(s) 30
MwoI GCNNNNNNNGC 4 cut(s) 188, 228, 506, 731
NciI CCSGG 2 cut(s) 263, 330
NcoI CCATGG 1 cut(s) 476
NdeII GATC 6 cut(s) 286, 430, 493, 598, 769, 789
NlaIII CATG 8 cut(s) 235, 313, 445, 480, 634, 729, 790, 812
NlaIV GGNNCC 2 cut(s) 53, 600
NmuCI GTSAC 1 cut(s) 743
NsiI ATGCAT 1 cut(s) 511
NspI RCATGY 2 cut(s) 313, 729
PagI TCATGA 1 cut(s) 786
PciI ACATGT 1 cut(s) 309
PdmI GAANNNNTTC 1 cut(s) 762
PfeI GAWTC 3 cut(s) 80, 134, 531
PflFI GACNNNGTC 1 cut(s) 116
PkrI GCNGC 3 cut(s) 72, 356, 424
PleI GAGTC 1 cut(s) 750
PpsI GAGTC 1 cut(s) 750
PscI ACATGT 1 cut(s) 309
Psp124BI GAGCTC 2 cut(s) 184, 414
Psp6I CCWGG 1 cut(s) 661
PspGI CCWGG 1 cut(s) 661
PspN4I GGNNCC 2 cut(s) 53, 600
PspPI GGNCC 1 cut(s) 52
PsuI RGATCY 1 cut(s) 598
PsyI GACNNNGTC 1 cut(s) 116
PvuII CAGCTG 1 cut(s) 77
RsaI GTAC 1 cut(s) 708
RsaNI GTAC 1 cut(s) 707
SacI GAGCTC 2 cut(s) 184, 414
SaqAI TTAA 5 cut(s) 140, 147, 314, 653, 777
SatI GCNGC 3 cut(s) 71, 355, 423
Sau3AI GATC 6 cut(s) 286, 430, 493, 598, 769, 789
Sau96I GGNCC 1 cut(s) 52
SchI GAGTC 1 cut(s) 751
ScrFI CCNGG 3 cut(s) 263, 330, 663
SduI GDGCHC 4 cut(s) 184, 414, 612, 756
SfaNI GCATC 1 cut(s) 518
SmlI CTYRAG 1 cut(s) 800
SmoI CTYRAG 1 cut(s) 800
Sse9I AATT 3 cut(s) 549, 656, 761
SsiI CCGC 5 cut(s) 28, 334, 355, 535, 612
SstI GAGCTC 2 cut(s) 184, 414
StyD4I CCNGG 3 cut(s) 261, 328, 661
StyI CCWWGG 2 cut(s) 55, 476
TaaI ACNGT 3 cut(s) 241, 373, 684
TasI AATT 3 cut(s) 549, 656, 761
TauI GCSGC 1 cut(s) 357
TfiI GAWTC 3 cut(s) 80, 134, 531
Tru1I TTAA 5 cut(s) 140, 147, 314, 653, 777
Tru9I TTAA 5 cut(s) 140, 147, 314, 653, 777
TscAI CASTG 3 cut(s) 378, 627, 699
TseFI GTSAC 1 cut(s) 743
TseI GCWGC 2 cut(s) 70, 422
Tsp45I GTSAC 1 cut(s) 743
TspDTI ATGAA 1 cut(s) 775
TspGWI ACGGA 1 cut(s) 600
TspRI CASTG 3 cut(s) 378, 627, 699
Tth111I GACNNNGTC 1 cut(s) 116
VneI GTGCAC 1 cut(s) 752
XapI RAATTY 1 cut(s) 656
XceI RCATGY 2 cut(s) 313, 729
XmnI GAANNNNTTC 1 cut(s) 762
Zsp2I ATGCAT 1 cut(s) 511
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.