MD17G1243800.v1.1

Bifunctional epoxide hydrolase 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0001763
Physical Location & Seq
Reverse (-)
29239658 .. 29241509
1852 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1243800.v1.1.491

Sequence Viewer

Length: 879 bp
ATGGGTCCAAAAGTGGTGCTGTTCTTGCATGGATTTCCAGAGATATGGTACACATGGAGGCATCAAATGGTTGCTGTCGCAGACAAAGGCTACCGGGCGATATCCATTGACTTCAGAGGCTTTGGGCTTTCCGAGCAGCCGGCTGAACCCGGAAAGGCAACCTGGAAAGACCTTGTTGACGATGTCGTTGACCTTCTTGATTCTGTGAGCATTAACAAGGTTGTTCTTGTTGGGAAGGATTTTGGAGCTCTGCCTGCATACCTAACACCTGCTCTCCACCCAGAGCGTGTAGCCGGCATGATAACAGTAGGTGTTCCTTTCCTCAACCCGGCTAACTCTCCTGTCCGATTTGATCTCTTACCTGAAGGCTTCTACATGTCAAGATGGAAGGAGCCGGGGCGGGCAGAAGCAGATTTTGGCCGCTTCGATGTCAAGACCGTGATACGAAACATCTACATTCTCTTCTCCGGGAGTGAGCTCCAAGTAGCCGCTCCTGATCAAGAAATCATGGACTTGGTAGACCCTGCCACTCCTCTACCACCATGGTTTTCGGAGGAAGATCTTGCAGTCTATGCATCTCTATATGAGAACTCTGGATTCCGTTATCCCATCCAAGTTACATACCAAACTAAGGTCGTGGATTACGGATTAAGCAGCCATCCAAAAGTGCCGGCTCCATCACTGCTTATCATGGGTGAGAAGGACTACTTCCTGAAATTTCCGGGAGTGGAGGACTACTTACGGACTGGAGCAGTGAAGCATTTTGTCCCTGATTTGGACATTGCATATATTGCAGAAGGGAGCCACTTTGTGCACGAACAATTTCCTGATCAAGTTAATCAGCTCATCATCAGTTTCCTCGAAAAACATGGTATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000287 GO:0001676 GO:0003008 GO:0003013 GO:0003018 GO:0003674 GO:0003824 GO:0004301 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005782 GO:0005829 GO:0006082 GO:0006605 GO:0006625 GO:0006629 GO:0006631 GO:0006633 GO:0006690 GO:0006725 GO:0006793 GO:0006796 GO:0006805 GO:0006810 GO:0006873 GO:0006874 GO:0006875 GO:0006886 GO:0006950 GO:0006952 GO:0006954 GO:0006996 GO:0007031 GO:0008015 GO:0008104 GO:0008150 GO:0008152 GO:0008217 GO:0008610 GO:0009056 GO:0009058 GO:0009410 GO:0009636 GO:0009810 GO:0009893 GO:0009987 GO:0010468 GO:0010604 GO:0010628 GO:0015031 GO:0015643 GO:0015833 GO:0016043 GO:0016053 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0016801 GO:0016803 GO:0017144 GO:0018904 GO:0019216 GO:0019218 GO:0019222 GO:0019369 GO:0019373 GO:0019439 GO:0019725 GO:0019752 GO:0030003 GO:0030258 GO:0031907 GO:0031974 GO:0032501 GO:0032787 GO:0033036 GO:0033365 GO:0033559 GO:0034613 GO:0035150 GO:0035296 GO:0042221 GO:0042577 GO:0042578 GO:0042579 GO:0042592 GO:0042632 GO:0042759 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043574 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046272 GO:0046394 GO:0046483 GO:0046839 GO:0046872 GO:0046907 GO:0046983 GO:0048518 GO:0048878 GO:0050789 GO:0050801 GO:0050880 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0055088 GO:0055092 GO:0060255 GO:0062012 GO:0065007 GO:0065008 GO:0070013 GO:0070727 GO:0070887 GO:0071466 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072330 GO:0072503 GO:0072507 GO:0072593 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0090066 GO:0090181 GO:0097176 GO:0097746 GO:0097755 GO:0098771 GO:1900673 GO:1901360 GO:1901361 GO:1901568 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

293

Amino Acids

32.78

Weight (kDa)

5.01

Isoelectric Point (pI)

36.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 4 - 110 2.3e-10 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 5 - 113 1.2e-20 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 6 - 281 4.6e-17 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 277
Acc36I ACCTGC 1 cut(s) 277
AccBSI CCGCTC 1 cut(s) 491
AccI GTMKAC 1 cut(s) 519
AciI CCGC 3 cut(s) 400, 421, 489
AcoI YGGCCR 1 cut(s) 418
AcsI RAATTY 1 cut(s) 716
AcuI CTGAAG 2 cut(s) 97, 384
AdeI CACNNNGTG 1 cut(s) 811
AfaI GTAC 1 cut(s) 50
AfiI CCNNNNNNNGG 3 cut(s) 328, 631, 775
AflIII ACRYGT 1 cut(s) 375
AjnI CCWGG 1 cut(s) 161
AjuI GAANNNNNNNTTGG 2 cut(s) 399, 431
AluBI AGCT 3 cut(s) 248, 478, 844
AluI AGCT 3 cut(s) 248, 478, 844
Alw21I GWGCWC 3 cut(s) 250, 480, 816
Alw44I GTGCAC 1 cut(s) 812
AoxI GGCC 1 cut(s) 418
ApaLI GTGCAC 1 cut(s) 812
ApeKI GCWGC 2 cut(s) 136, 654
ApoI RAATTY 1 cut(s) 716
Asp700I GAANNNNTTC 1 cut(s) 822
AspS9I GGNCC 1 cut(s) 5
AsuC2I CCSGG 6 cut(s) 95, 150, 329, 396, 469, 723
AsuHPI GGTGA 1 cut(s) 707
AvaII GGWCC 1 cut(s) 5
BaeGI GKGCMC 1 cut(s) 816
BanII GRGCYC 2 cut(s) 250, 480
Bbv12I GWGCWC 3 cut(s) 250, 480, 816
BbvI GCAGC 2 cut(s) 148, 666
BccI CCATC 4 cut(s) 378, 617, 666, 685
BciT130I CCWGG 1 cut(s) 163
BclI TGATCA 2 cut(s) 496, 829
BcnI CCSGG 6 cut(s) 95, 150, 329, 396, 469, 723
BfuAI ACCTGC 1 cut(s) 277
BglII AGATCT 1 cut(s) 559
BisI GCNGC 4 cut(s) 137, 421, 489, 655
BlsI GCNGC 4 cut(s) 138, 422, 490, 656
Bme1390I CCNGG 7 cut(s) 95, 150, 163, 329, 396, 469, 723
Bme18I GGWCC 1 cut(s) 5
BmgT120I GGNCC 1 cut(s) 5
BmiI GGNNCC 4 cut(s) 6, 393, 675, 803
BmrFI CCNGG 7 cut(s) 95, 150, 163, 329, 396, 469, 723
BmsI GCATC 2 cut(s) 70, 584
BpmI CTGGAG 1 cut(s) 768
BpuMI CCSGG 6 cut(s) 95, 150, 329, 396, 469, 723
BsaJI CCNNGG 2 cut(s) 395, 542
BsaXI ACNNNNNCTCC 2 cut(s) 258, 288
Bsc4I CCNNNNNNNGG 3 cut(s) 328, 631, 775
Bse118I RCCGGY 3 cut(s) 139, 293, 670
Bse1I ACTGG 1 cut(s) 751
Bse3DI GCAATG 1 cut(s) 780
BseBI CCWGG 1 cut(s) 163
BseDI CCNNGG 2 cut(s) 395, 542
BseGI GGATG 2 cut(s) 609, 658
BseLI CCNNNNNNNGG 3 cut(s) 328, 631, 775
BseMI GCAATG 1 cut(s) 780
BseNI ACTGG 1 cut(s) 751
BseRI GAGGAG 1 cut(s) 522
BseSI GKGCMC 1 cut(s) 816
BseXI GCAGC 2 cut(s) 148, 666
BshFI GGCC 1 cut(s) 420
BsiHKAI GWGCWC 3 cut(s) 250, 480, 816
BsiSI CCGG 9 cut(s) 94, 140, 150, 294, 329, 395, 468, 671, 722
BslFI GGGAC 1 cut(s) 752
BslI CCNNNNNNNGG 3 cut(s) 328, 631, 775
BsmFI GGGAC 1 cut(s) 752
BsnI GGCC 1 cut(s) 420
Bsp1286I GDGCHC 3 cut(s) 250, 480, 816
Bsp143I GATC 4 cut(s) 352, 496, 559, 829
Bsp19I CCATGG 1 cut(s) 542
BspACI CCGC 3 cut(s) 400, 421, 489
BspANI GGCC 1 cut(s) 420
BspLI GGNNCC 4 cut(s) 6, 393, 675, 803
BspMI ACCTGC 1 cut(s) 277
BsrBI CCGCTC 1 cut(s) 491
BsrDI GCAATG 1 cut(s) 780
BsrFI RCCGGY 3 cut(s) 139, 293, 670
BsrI ACTGG 1 cut(s) 751
BssAI RCCGGY 3 cut(s) 139, 293, 670
BssECI CCNNGG 2 cut(s) 395, 542
BssMI GATC 4 cut(s) 352, 496, 559, 829
BssT1I CCWWGG 1 cut(s) 542
Bst2UI CCWGG 1 cut(s) 163
Bst4CI ACNGT 2 cut(s) 307, 439
Bst6I CTCTTC 1 cut(s) 467
BstAPI GCANNNNNTGC 2 cut(s) 572, 791
BstC8I GCNNGC 5 cut(s) 141, 255, 295, 402, 672
BstDEI CTNAG 1 cut(s) 630
BstDSI CCRYGG 1 cut(s) 542
BstF5I GGATG 2 cut(s) 609, 658
BstKTI GATC 4 cut(s) 355, 499, 562, 832
BstMBI GATC 4 cut(s) 352, 496, 559, 829
BstMWI GCNNNNNNNGC 5 cut(s) 25, 133, 254, 572, 791
BstNI CCWGG 1 cut(s) 163
BstNSI RCATGY 1 cut(s) 379
BstSCI CCNGG 7 cut(s) 93, 148, 161, 327, 394, 467, 721
BstSLI GKGCMC 1 cut(s) 816
BstV1I GCAGC 2 cut(s) 148, 666
BstX2I RGATCY 1 cut(s) 559
BstXI CCANNNNNNTGG 1 cut(s) 45
BstYI RGATCY 1 cut(s) 559
BsuRI GGCC 1 cut(s) 420
BtgI CCRYGG 1 cut(s) 542
BtsCI GGATG 2 cut(s) 609, 658
BtsI GCAGTG 2 cut(s) 680, 759
BtsIMutI CAGTG 2 cut(s) 680, 759
BveI ACCTGC 1 cut(s) 277
Cac8I GCNNGC 5 cut(s) 141, 255, 295, 402, 672
Cfr10I RCCGGY 3 cut(s) 139, 293, 670
Cfr13I GGNCC 1 cut(s) 5
Csp6I GTAC 1 cut(s) 49
CviAII CATG 8 cut(s) 29, 54, 298, 376, 508, 543, 691, 869
CviQI GTAC 1 cut(s) 49
DdeI CTNAG 1 cut(s) 630
DpnI GATC 4 cut(s) 354, 498, 561, 831
DpnII GATC 4 cut(s) 352, 496, 559, 829
DraIII CACNNNGTG 1 cut(s) 811
EaeI YGGCCR 1 cut(s) 418
Eam1104I CTCTTC 1 cut(s) 467
EarI CTCTTC 1 cut(s) 467
Ecl136II GAGCTC 2 cut(s) 248, 478
Eco130I CCWWGG 1 cut(s) 542
Eco24I GRGCYC 2 cut(s) 250, 480
Eco32I GATATC 1 cut(s) 102
Eco47I GGWCC 1 cut(s) 5
Eco53kI GAGCTC 2 cut(s) 248, 478
Eco57I CTGAAG 2 cut(s) 97, 384
EcoICRI GAGCTC 2 cut(s) 248, 478
EcoRII CCWGG 1 cut(s) 161
EcoRV GATATC 1 cut(s) 102
EcoT14I CCWWGG 1 cut(s) 542
EcoT22I ATGCAT 1 cut(s) 577
EcoT38I GRGCYC 2 cut(s) 250, 480
ErhI CCWWGG 1 cut(s) 542
FaeI CATG 8 cut(s) 32, 57, 301, 379, 511, 546, 694, 872
FalI AAGNNNNNCTT 2 cut(s) 692, 724
FaqI GGGAC 1 cut(s) 752
FatI CATG 8 cut(s) 28, 53, 297, 375, 507, 542, 690, 868
FauI CCCGC 1 cut(s) 393
FbaI TGATCA 2 cut(s) 496, 829
FblI GTMKAC 1 cut(s) 519
Fnu4HI GCNGC 4 cut(s) 137, 421, 489, 655
FokI GGATG 2 cut(s) 596, 645
FriOI GRGCYC 2 cut(s) 250, 480
Fsp4HI GCNGC 4 cut(s) 137, 421, 489, 655
GluI GCNGC 4 cut(s) 137, 421, 489, 655
GsuI CTGGAG 1 cut(s) 768
HaeIII GGCC 1 cut(s) 420
HapII CCGG 9 cut(s) 94, 140, 150, 294, 329, 395, 468, 671, 722
Hin1II CATG 8 cut(s) 32, 57, 301, 379, 511, 546, 694, 872
HincII GTYRAC 2 cut(s) 178, 190
HindII GTYRAC 2 cut(s) 178, 190
HinfI GANTC 2 cut(s) 200, 597
HpaII CCGG 9 cut(s) 94, 140, 150, 294, 329, 395, 468, 671, 722
HphI GGTGA 1 cut(s) 707
Hpy166II GTNNAC 5 cut(s) 51, 178, 190, 520, 814
Hpy188I TCNGA 4 cut(s) 116, 133, 347, 553
Hpy188III TCNNGA 9 cut(s) 38, 197, 381, 433, 494, 500, 594, 712, 827
Hpy8I GTNNAC 5 cut(s) 51, 178, 190, 520, 814
HpyAV CCTTC 6 cut(s) 203, 229, 359, 382, 694, 791
HpyCH4III ACNGT 2 cut(s) 307, 439
HpyCH4V TGCA 7 cut(s) 28, 257, 566, 575, 785, 794, 814
HpyF10VI GCNNNNNNNGC 5 cut(s) 25, 133, 254, 572, 791
HpyF3I CTNAG 1 cut(s) 630
Hsp92II CATG 8 cut(s) 32, 57, 301, 379, 511, 546, 694, 872
KroI GCCGGC 3 cut(s) 139, 293, 670
KroNI GCCGGC 3 cut(s) 141, 295, 672
Ksp22I TGATCA 2 cut(s) 496, 829
Kzo9I GATC 4 cut(s) 352, 496, 559, 829
LmnI GCTCC 7 cut(s) 245, 391, 483, 496, 679, 749, 801
Lsp1109I GCAGC 2 cut(s) 148, 666
LweI GCATC 2 cut(s) 70, 584
MaeIII GTNAC 1 cut(s) 616
MalI GATC 4 cut(s) 354, 498, 561, 831
MbiI CCGCTC 1 cut(s) 491
MboI GATC 4 cut(s) 352, 496, 559, 829
MboII GAAGA 2 cut(s) 454, 569
MflI RGATCY 1 cut(s) 559
MhlI GDGCHC 3 cut(s) 250, 480, 816
MluCI AATT 2 cut(s) 716, 821
MnlI CCTC 7 cut(s) 51, 110, 332, 543, 547, 724, 869
Mph1103I ATGCAT 1 cut(s) 577
MroNI GCCGGC 3 cut(s) 139, 293, 670
MroXI GAANNNNTTC 1 cut(s) 822
MseI TTAA 3 cut(s) 213, 650, 837
MspI CCGG 9 cut(s) 94, 140, 150, 294, 329, 395, 468, 671, 722
MspR9I CCNGG 7 cut(s) 95, 150, 163, 329, 396, 469, 723
MvaI CCWGG 1 cut(s) 163
MwoI GCNNNNNNNGC 5 cut(s) 25, 133, 254, 572, 791
NaeI GCCGGC 3 cut(s) 141, 295, 672
NciI CCSGG 6 cut(s) 95, 150, 329, 396, 469, 723
NcoI CCATGG 1 cut(s) 542
NdeII GATC 4 cut(s) 352, 496, 559, 829
NgoMIV GCCGGC 3 cut(s) 139, 293, 670
NlaIII CATG 8 cut(s) 32, 57, 301, 379, 511, 546, 694, 872
NlaIV GGNNCC 4 cut(s) 6, 393, 675, 803
NsiI ATGCAT 1 cut(s) 577
NspI RCATGY 1 cut(s) 379
PaqCI CACCTGC 1 cut(s) 277
PciI ACATGT 1 cut(s) 375
PdiI GCCGGC 3 cut(s) 141, 295, 672
PdmI GAANNNNTTC 1 cut(s) 822
PfeI GAWTC 2 cut(s) 200, 597
PflFI GACNNNGTC 1 cut(s) 182
PfoI TCCNGGA 2 cut(s) 467, 721
PkrI GCNGC 4 cut(s) 138, 422, 490, 656
PscI ACATGT 1 cut(s) 375
Psp124BI GAGCTC 2 cut(s) 250, 480
Psp6I CCWGG 1 cut(s) 161
PspGI CCWGG 1 cut(s) 161
PspN4I GGNNCC 4 cut(s) 6, 393, 675, 803
PspPI GGNCC 1 cut(s) 5
PsuI RGATCY 1 cut(s) 559
PsyI GACNNNGTC 1 cut(s) 182
RsaI GTAC 1 cut(s) 50
RsaNI GTAC 1 cut(s) 49
SacI GAGCTC 2 cut(s) 250, 480
SaqAI TTAA 3 cut(s) 213, 650, 837
SatI GCNGC 4 cut(s) 137, 421, 489, 655
Sau3AI GATC 4 cut(s) 352, 496, 559, 829
Sau96I GGNCC 1 cut(s) 5
ScrFI CCNGG 7 cut(s) 95, 150, 163, 329, 396, 469, 723
SduI GDGCHC 3 cut(s) 250, 480, 816
SfaNI GCATC 2 cut(s) 70, 584
SinI GGWCC 1 cut(s) 5
Sse9I AATT 2 cut(s) 716, 821
SsiI CCGC 3 cut(s) 400, 421, 489
SstI GAGCTC 2 cut(s) 250, 480
StyD4I CCNGG 7 cut(s) 93, 148, 161, 327, 394, 467, 721
StyI CCWWGG 1 cut(s) 542
TaaI ACNGT 2 cut(s) 307, 439
TaqI TCGA 2 cut(s) 426, 861
TasI AATT 2 cut(s) 716, 821
TauI GCSGC 2 cut(s) 423, 491
TfiI GAWTC 2 cut(s) 200, 597
Tru1I TTAA 3 cut(s) 213, 650, 837
Tru9I TTAA 3 cut(s) 213, 650, 837
TscAI CASTG 2 cut(s) 687, 759
TseI GCWGC 2 cut(s) 136, 654
TspGWI ACGGA 3 cut(s) 590, 660, 757
TspRI CASTG 2 cut(s) 687, 759
Tth111I GACNNNGTC 1 cut(s) 182
VneI GTGCAC 1 cut(s) 812
VpaK11BI GGWCC 1 cut(s) 5
XapI RAATTY 1 cut(s) 716
XceI RCATGY 1 cut(s) 379
XmiI GTMKAC 1 cut(s) 519
XmnI GAANNNNTTC 1 cut(s) 822
Zsp2I ATGCAT 1 cut(s) 577
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.