pycom09g16800

Bifunctional epoxide hydrolase 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0001763
Physical Location & Seq
Reverse (-)
17181081 .. 17182453
1373 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g16800.4

Sequence Viewer

Length: 633 bp
ATGGAAAATATCCAGCACAAACATGTGGAAGTAAGAGGACTCAAGCTTCACGTAGCCGAAATCGGAAGTGGTCCAAAGGCTGTGGTGTTCCTTCATGGATTTCCAGAAATATGGTACACATGGAGGCATCAAATGGTTGCGATGGCGAAAAGAAGCTACCGGGCAATAGCCATTGATTTCAGGGGCTATGGACTTTCCGAGCAGCCGGCTGAACCTGAGAAGGACACCTTCATTGACCTTGTCCACGATATCGTTGCCCTTTTGGATTCTTTGGCAATTAACAAGACTTTTCTTATTGGGAAAGATTTTGGAGCATTGCCTGCATACTTAGTAGCTGCTCTCCACCCGGATCGGGTATCTGGCGTCGTTACACTTGGTGTTCCTTTTATGCTACCAGGTCCCTCTGCTATCCAAAATCATCTCCTCCCTGAAGGCTTCTACATATCAAGGTGGCAGGAGCCAGGGCGGGCAGAAGCAGATTTTGGCCGCTTTGATGTGAAGACGGTGATTAGGAACATCTACATTCTCTTCTCTAGAAGCGACATCCCAATAGCTGCCCCTGATCAAGAAATCATGGACCTGTTTGACCCTGCAACTCCTCTACCGCCAGGGTTTTCGGAAGAACGATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000287 GO:0001676 GO:0003008 GO:0003013 GO:0003018 GO:0003674 GO:0003824 GO:0004301 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005782 GO:0005829 GO:0006082 GO:0006605 GO:0006625 GO:0006629 GO:0006631 GO:0006633 GO:0006690 GO:0006725 GO:0006793 GO:0006796 GO:0006805 GO:0006810 GO:0006873 GO:0006874 GO:0006875 GO:0006886 GO:0006950 GO:0006952 GO:0006954 GO:0006996 GO:0007031 GO:0008015 GO:0008104 GO:0008150 GO:0008152 GO:0008217 GO:0008610 GO:0009056 GO:0009058 GO:0009410 GO:0009636 GO:0009810 GO:0009893 GO:0009987 GO:0010468 GO:0010604 GO:0010628 GO:0015031 GO:0015643 GO:0015833 GO:0016043 GO:0016053 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0016801 GO:0016803 GO:0017144 GO:0018904 GO:0019216 GO:0019218 GO:0019222 GO:0019369 GO:0019373 GO:0019439 GO:0019725 GO:0019752 GO:0030003 GO:0030258 GO:0031907 GO:0031974 GO:0032501 GO:0032787 GO:0033036 GO:0033365 GO:0033559 GO:0034613 GO:0035150 GO:0035296 GO:0042221 GO:0042577 GO:0042578 GO:0042579 GO:0042592 GO:0042632 GO:0042759 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043574 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046272 GO:0046394 GO:0046483 GO:0046839 GO:0046872 GO:0046907 GO:0046983 GO:0048518 GO:0048878 GO:0050789 GO:0050801 GO:0050880 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0055088 GO:0055092 GO:0060255 GO:0062012 GO:0065007 GO:0065008 GO:0070013 GO:0070727 GO:0070887 GO:0071466 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072330 GO:0072503 GO:0072507 GO:0072593 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0090066 GO:0090181 GO:0097176 GO:0097746 GO:0097755 GO:0098771 GO:1900673 GO:1901360 GO:1901361 GO:1901568 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

211

Amino Acids

23.5

Weight (kDa)

5.73

Isoelectric Point (pI)

52.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 466, 487, 605
AclWI GGATC 1 cut(s) 357
AcoI YGGCCR 1 cut(s) 484
AcuI CTGAAG 1 cut(s) 450
AcyI GRCGYC 1 cut(s) 363
AdeI CACNNNGTG 1 cut(s) 377
AfaI GTAC 1 cut(s) 116
AfiI CCNNNNNNNGG 1 cut(s) 352
AflIII ACRYGT 1 cut(s) 22
AjnI CCWGG 3 cut(s) 394, 460, 607
AjuI GAANNNNNNNTTGG 2 cut(s) 465, 497
AluBI AGCT 4 cut(s) 46, 156, 335, 554
AluI AGCT 4 cut(s) 46, 156, 335, 554
AlwI GGATC 1 cut(s) 357
AoxI GGCC 1 cut(s) 484
ApeKI GCWGC 3 cut(s) 202, 335, 554
AspS9I GGNCC 3 cut(s) 71, 398, 577
AsuC2I CCSGG 2 cut(s) 161, 347
AsuHPI GGTGA 1 cut(s) 517
AvaII GGWCC 3 cut(s) 71, 398, 577
BbsI GAAGAC 1 cut(s) 506
BbvI GCAGC 3 cut(s) 214, 322, 541
BccI CCATC 1 cut(s) 136
BcgI CGANNNNNNTGC 2 cut(s) 236, 270
BciT130I CCWGG 3 cut(s) 396, 462, 609
BclI TGATCA 1 cut(s) 562
BcnI CCSGG 2 cut(s) 161, 347
BfaI CTAG 1 cut(s) 534
BisI GCNGC 4 cut(s) 203, 336, 487, 555
BlsI GCNGC 4 cut(s) 204, 337, 488, 556
Bme1390I CCNGG 5 cut(s) 161, 347, 396, 462, 609
Bme18I GGWCC 3 cut(s) 71, 398, 577
BmgT120I GGNCC 3 cut(s) 71, 398, 577
BmiI GGNNCC 2 cut(s) 400, 459
BmrFI CCNGG 5 cut(s) 161, 347, 396, 462, 609
BmsI GCATC 1 cut(s) 136
BpiI GAAGAC 1 cut(s) 506
BpuEI CTTGAG 1 cut(s) 26
BpuMI CCSGG 2 cut(s) 161, 347
BsaAI YACGTR 1 cut(s) 52
BsaHI GRCGYC 1 cut(s) 363
BsaJI CCNNGG 2 cut(s) 461, 608
Bsc4I CCNNNNNNNGG 1 cut(s) 352
Bse118I RCCGGY 1 cut(s) 205
Bse3DI GCAATG 1 cut(s) 314
BseBI CCWGG 3 cut(s) 396, 462, 609
BseDI CCNNGG 2 cut(s) 461, 608
BseGI GGATG 1 cut(s) 543
BseLI CCNNNNNNNGG 1 cut(s) 352
BseMI GCAATG 1 cut(s) 314
BseMII CTCAG 1 cut(s) 207
BseRI GAGGAG 2 cut(s) 413, 588
BseXI GCAGC 3 cut(s) 214, 322, 541
BshFI GGCC 1 cut(s) 486
BsiSI CCGG 3 cut(s) 160, 206, 347
BslFI GGGAC 1 cut(s) 384
BslI CCNNNNNNNGG 1 cut(s) 352
BsmFI GGGAC 1 cut(s) 384
BsnI GGCC 1 cut(s) 486
Bsp143I GATC 3 cut(s) 349, 562, 626
BspACI CCGC 3 cut(s) 466, 487, 605
BspANI GGCC 1 cut(s) 486
BspCNI CTCAG 1 cut(s) 208
BspLI GGNNCC 2 cut(s) 400, 459
BspPI GGATC 1 cut(s) 357
BsrDI GCAATG 1 cut(s) 314
BsrFI RCCGGY 1 cut(s) 205
BssAI RCCGGY 1 cut(s) 205
BssECI CCNNGG 2 cut(s) 461, 608
BssMI GATC 3 cut(s) 349, 562, 626
BssNI GRCGYC 1 cut(s) 363
Bst2UI CCWGG 3 cut(s) 396, 462, 609
Bst4CI ACNGT 1 cut(s) 505
Bst6I CTCTTC 1 cut(s) 533
BstACI GRCGYC 1 cut(s) 363
BstAPI GCANNNNNTGC 1 cut(s) 320
BstBAI YACGTR 1 cut(s) 52
BstC8I GCNNGC 3 cut(s) 207, 321, 468
BstDEI CTNAG 2 cut(s) 216, 328
BstF5I GGATG 1 cut(s) 543
BstKTI GATC 3 cut(s) 352, 565, 629
BstMBI GATC 3 cut(s) 349, 562, 626
BstMWI GCNNNNNNNGC 1 cut(s) 320
BstNI CCWGG 3 cut(s) 396, 462, 609
BstNSI RCATGY 1 cut(s) 26
BstSCI CCNGG 5 cut(s) 159, 345, 394, 460, 607
BstV1I GCAGC 3 cut(s) 214, 322, 541
BstV2I GAAGAC 1 cut(s) 506
BstXI CCANNNNNNTGG 1 cut(s) 111
BsuRI GGCC 1 cut(s) 486
BtgZI GCGATG 1 cut(s) 155
BtsCI GGATG 1 cut(s) 543
Cac8I GCNNGC 3 cut(s) 207, 321, 468
Cfr10I RCCGGY 1 cut(s) 205
Cfr13I GGNCC 3 cut(s) 71, 398, 577
CseI GACGC 1 cut(s) 352
CsiI ACCWGGT 1 cut(s) 394
Csp6I GTAC 1 cut(s) 115
CspCI CAANNNNNGTGG 2 cut(s) 63, 98
CviAII CATG 4 cut(s) 23, 95, 120, 574
CviQI GTAC 1 cut(s) 115
DdeI CTNAG 2 cut(s) 216, 328
DpnI GATC 3 cut(s) 351, 564, 628
DpnII GATC 3 cut(s) 349, 562, 626
DraIII CACNNNGTG 1 cut(s) 377
EaeI YGGCCR 1 cut(s) 484
Eam1104I CTCTTC 1 cut(s) 533
EarI CTCTTC 1 cut(s) 533
Eco32I GATATC 1 cut(s) 250
Eco47I GGWCC 3 cut(s) 71, 398, 577
Eco57I CTGAAG 1 cut(s) 450
EcoO109I RGGNCCY 1 cut(s) 398
EcoRII CCWGG 3 cut(s) 394, 460, 607
EcoRV GATATC 1 cut(s) 250
FaeI CATG 4 cut(s) 26, 98, 123, 577
FaiI YATR 9 cut(s) 24, 96, 112, 121, 189, 325, 389, 443, 575
FalI AAGNNNNNCTT 2 cut(s) 212, 244
FaqI GGGAC 1 cut(s) 384
FatI CATG 4 cut(s) 22, 94, 119, 573
FauI CCCGC 1 cut(s) 459
FbaI TGATCA 1 cut(s) 562
Fnu4HI GCNGC 4 cut(s) 203, 336, 487, 555
FokI GGATG 1 cut(s) 530
Fsp4HI GCNGC 4 cut(s) 203, 336, 487, 555
FspBI CTAG 1 cut(s) 534
GluI GCNGC 4 cut(s) 203, 336, 487, 555
HaeIII GGCC 1 cut(s) 486
HapII CCGG 3 cut(s) 160, 206, 347
HgaI GACGC 1 cut(s) 352
Hin1I GRCGYC 1 cut(s) 363
Hin1II CATG 4 cut(s) 26, 98, 123, 577
HindIII AAGCTT 1 cut(s) 44
HinfI GANTC 2 cut(s) 39, 266
HpaII CCGG 3 cut(s) 160, 206, 347
HphI GGTGA 1 cut(s) 517
Hpy166II GTNNAC 2 cut(s) 117, 244
Hpy188I TCNGA 3 cut(s) 65, 199, 619
Hpy188III TCNNGA 4 cut(s) 104, 534, 566, 630
Hpy8I GTNNAC 2 cut(s) 117, 244
Hpy99I CGWCG 1 cut(s) 368
HpyAV CCTTC 4 cut(s) 101, 214, 238, 425
HpyCH4III ACNGT 1 cut(s) 505
HpyCH4IV ACGT 1 cut(s) 51
HpyCH4V TGCA 2 cut(s) 323, 593
HpyF10VI GCNNNNNNNGC 1 cut(s) 320
HpyF3I CTNAG 2 cut(s) 216, 328
HpySE526I ACGT 1 cut(s) 51
Hsp92I GRCGYC 1 cut(s) 363
Hsp92II CATG 4 cut(s) 26, 98, 123, 577
KroI GCCGGC 1 cut(s) 205
KroNI GCCGGC 1 cut(s) 207
Ksp22I TGATCA 1 cut(s) 562
Kzo9I GATC 3 cut(s) 349, 562, 626
LmnI GCTCC 2 cut(s) 311, 457
Lsp1109I GCAGC 3 cut(s) 214, 322, 541
LweI GCATC 1 cut(s) 136
MabI ACCWGGT 1 cut(s) 394
MaeI CTAG 1 cut(s) 534
MaeII ACGT 1 cut(s) 51
MaeIII GTNAC 1 cut(s) 367
MalI GATC 3 cut(s) 351, 564, 628
MboI GATC 3 cut(s) 349, 562, 626
MboII GAAGA 3 cut(s) 511, 520, 632
MluCI AATT 1 cut(s) 276
MlyI GAGTC 1 cut(s) 33
MnlI CCTC 5 cut(s) 29, 117, 412, 434, 609
MroNI GCCGGC 1 cut(s) 205
MseI TTAA 1 cut(s) 279
MslI CAYNNNNRTG 1 cut(s) 21
MspI CCGG 3 cut(s) 160, 206, 347
MspR9I CCNGG 5 cut(s) 161, 347, 396, 462, 609
MvaI CCWGG 3 cut(s) 396, 462, 609
MwoI GCNNNNNNNGC 1 cut(s) 320
NaeI GCCGGC 1 cut(s) 207
NciI CCSGG 2 cut(s) 161, 347
NdeII GATC 3 cut(s) 349, 562, 626
NgoMIV GCCGGC 1 cut(s) 205
NlaIII CATG 4 cut(s) 26, 98, 123, 577
NlaIV GGNNCC 2 cut(s) 400, 459
NspI RCATGY 1 cut(s) 26
PciI ACATGT 1 cut(s) 22
PdiI GCCGGC 1 cut(s) 207
PfeI GAWTC 1 cut(s) 266
PflFI GACNNNGTC 1 cut(s) 239
PkrI GCNGC 4 cut(s) 204, 337, 488, 556
PleI GAGTC 1 cut(s) 33
PpsI GAGTC 1 cut(s) 33
Ppu21I YACGTR 1 cut(s) 52
PpuMI RGGWCCY 1 cut(s) 398
PscI ACATGT 1 cut(s) 22
Psp5II RGGWCCY 1 cut(s) 398
Psp6I CCWGG 3 cut(s) 394, 460, 607
PspGI CCWGG 3 cut(s) 394, 460, 607
PspN4I GGNNCC 2 cut(s) 400, 459
PspPI GGNCC 3 cut(s) 71, 398, 577
PspPPI RGGWCCY 1 cut(s) 398
PsyI GACNNNGTC 1 cut(s) 239
RsaI GTAC 1 cut(s) 116
RsaNI GTAC 1 cut(s) 115
RseI CAYNNNNRTG 1 cut(s) 21
SaqAI TTAA 1 cut(s) 279
SatI GCNGC 4 cut(s) 203, 336, 487, 555
Sau3AI GATC 3 cut(s) 349, 562, 626
Sau96I GGNCC 3 cut(s) 71, 398, 577
SchI GAGTC 1 cut(s) 33
ScrFI CCNGG 5 cut(s) 161, 347, 396, 462, 609
SexAI ACCWGGT 1 cut(s) 394
SfaNI GCATC 1 cut(s) 136
SinI GGWCC 3 cut(s) 71, 398, 577
SmiMI CAYNNNNRTG 1 cut(s) 21
SmlI CTYRAG 1 cut(s) 41
SmoI CTYRAG 1 cut(s) 41
Sse9I AATT 1 cut(s) 276
SsiI CCGC 3 cut(s) 466, 487, 605
SspMI CTAG 1 cut(s) 534
StyD4I CCNGG 5 cut(s) 159, 345, 394, 460, 607
TaaI ACNGT 1 cut(s) 505
TaiI ACGT 1 cut(s) 54
TasI AATT 1 cut(s) 276
TauI GCSGC 1 cut(s) 489
TfiI GAWTC 1 cut(s) 266
Tru1I TTAA 1 cut(s) 279
Tru9I TTAA 1 cut(s) 279
TseI GCWGC 3 cut(s) 202, 335, 554
TspDTI ATGAA 2 cut(s) 83, 220
Tth111I GACNNNGTC 1 cut(s) 239
VpaK11BI GGWCC 3 cut(s) 71, 398, 577
XbaI TCTAGA 1 cut(s) 533
XceI RCATGY 1 cut(s) 26
XspI CTAG 1 cut(s) 534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.