Rw7G027430

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0001763
Physical Location & Seq
Reverse (-)
35457880 .. 35459400
1521 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G027430.1

Sequence Viewer

Length: 885 bp
ATGGAAAAGATCGAGCACAAGTATGTGGAAGTAGGAGGACTAAAGCTTCATGTAGCTGAAATTGGAAGTGGTCCAAAGGTGGTGCTTTTCCTGCATGGATTTCCAGAAATATGGTACTCGTGGAGGCACCAAATGGTTGCTCAGCCAGCTGAGCCTGAAAAGGCTACTTTCAATGACCTTGTTGAAGAGGTTCTTGGCCTTTTGGATTCTTTGGCCATTAACAAGGCTTTCCTTGTTGGTAAGGATTTTGGAGCTATGCCTGCATACATAGTAGCTGCTCTCCATCCTGATCGAGTAGCTGGTCTTATATCACTAGGTATTCCTTTTATGCTACCAGGTCCTACTGCTGTCCAAAATCATCTTCTTCCTGAAGGTTTCTATGTATCAAGGTGGCAGGAGCCAGCTGGGAGGGCAGAAGCAGATTTTGGCCGCTTTGATGTTAAGTCAGTGATAAGGAACATCTACATTCTCTTCTGTGGAAGTGAGGTTCCAGTAGCTGCTAAGGATCAAGAGATCATGGATTTGTTTGATCCAGCTATTCCTCTACCACCATGGTTCTCTGAAGAAGATCTTTCAGTCTATGCATCTCTTTATGAGAAGTCTGGATTCCGTTTTCCATTGCAAATTCCATACAGGAGTTTAGCAGTGGACTGCGGTTATACTGATCCAAAAGTCTCTGCTCCAACACTGCTTGTCATGGGTGAGAAAGACTACGTCTTAAAGATTCCGGGGTTTGGAGATTACATACGAACTGGGGCAGTGAAGCAATTTGTGCCTGATTTGGACATTAAATACATTGCAGAAGGGAATCATTTCGTGCAAGAACAACTTCCAGAGCAAATTAATCAGCTAATTCTCACTTTTCTAGGAAAACATGGGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

294

Amino Acids

32.79

Weight (kDa)

5.12

Isoelectric Point (pI)

41.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 56 - 117 1.8e-06 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 126
AciI CCGC 2 cut(s) 430, 654
AclWI GGATC 3 cut(s) 513, 524, 659
AcoI YGGCCR 2 cut(s) 213, 427
AcsI RAATTY 1 cut(s) 624
AcuI CTGAAG 2 cut(s) 390, 582
AfaI GTAC 1 cut(s) 116
AfiI CCNNNNNNNGG 1 cut(s) 734
AgsI TTSAA 2 cut(s) 172, 185
AjnI CCWGG 1 cut(s) 334
AjuI GAANNNNNNNTTGG 6 cut(s) 177, 209, 345, 377, 408, 440
AloI GAACNNNNNNTCC 2 cut(s) 471, 503
Alw21I GWGCWC 1 cut(s) 18
Alw26I GTCTC 1 cut(s) 679
AlwI GGATC 3 cut(s) 513, 524, 659
AlwNI CAGNNNCTG 1 cut(s) 497
AoxI GGCC 3 cut(s) 196, 213, 427
ApeKI GCWGC 2 cut(s) 275, 497
ApoI RAATTY 1 cut(s) 624
AseI ATTAAT 1 cut(s) 843
Asp700I GAANNNNTTC 3 cut(s) 189, 812, 828
AspS9I GGNCC 2 cut(s) 71, 338
AsuC2I CCSGG 1 cut(s) 729
AsuHPI GGTGA 1 cut(s) 713
AvaII GGWCC 2 cut(s) 71, 338
BalI TGGCCA 1 cut(s) 215
BanI GGYRCC 1 cut(s) 126
BauI CACGAG 1 cut(s) 118
Bbv12I GWGCWC 1 cut(s) 18
BbvI GCAGC 2 cut(s) 262, 484
BccI CCATC 1 cut(s) 291
BciT130I CCWGG 1 cut(s) 336
BcnI CCSGG 1 cut(s) 729
BcoDI GTCTC 1 cut(s) 679
BfaI CTAG 2 cut(s) 314, 866
BglII AGATCT 1 cut(s) 568
BisI GCNGC 3 cut(s) 276, 430, 498
BlpI GCTNAGC 2 cut(s) 141, 150
BlsI GCNGC 3 cut(s) 277, 431, 499
Bme1390I CCNGG 2 cut(s) 336, 729
Bme18I GGWCC 2 cut(s) 71, 338
BmgT120I GGNCC 2 cut(s) 71, 338
BmiI GGNNCC 3 cut(s) 128, 399, 489
BmrFI CCNGG 2 cut(s) 336, 729
BmrI ACTGGG 1 cut(s) 762
BmsI GCATC 1 cut(s) 593
BmuI ACTGGG 1 cut(s) 762
Bpu10I CCTNAGC 1 cut(s) 501
Bpu1102I GCTNAGC 2 cut(s) 141, 150
BpuMI CCSGG 1 cut(s) 729
BsaJI CCNNGG 2 cut(s) 551, 728
Bsc4I CCNNNNNNNGG 1 cut(s) 734
Bse1I ACTGG 2 cut(s) 491, 757
Bse3DI GCAATG 2 cut(s) 617, 795
BseBI CCWGG 1 cut(s) 336
BseDI CCNNGG 2 cut(s) 551, 728
BseGI GGATG 1 cut(s) 283
BseLI CCNNNNNNNGG 1 cut(s) 734
BseMI GCAATG 2 cut(s) 617, 795
BseMII CTCAG 2 cut(s) 141, 155
BseNI ACTGG 2 cut(s) 491, 757
BseXI GCAGC 2 cut(s) 262, 484
BseYI CCCAGC 1 cut(s) 404
BshFI GGCC 3 cut(s) 198, 215, 429
BshNI GGYRCC 1 cut(s) 126
BsiHKAI GWGCWC 1 cut(s) 18
BsiSI CCGG 1 cut(s) 728
BslI CCNNNNNNNGG 1 cut(s) 734
BsmAI GTCTC 1 cut(s) 679
BsnI GGCC 3 cut(s) 198, 215, 429
Bsp1286I GDGCHC 1 cut(s) 18
Bsp143I GATC 8 cut(s) 9, 289, 505, 513, 529, 568, 664, 879
Bsp1720I GCTNAGC 2 cut(s) 141, 150
Bsp19I CCATGG 1 cut(s) 551
BspACI CCGC 2 cut(s) 430, 654
BspANI GGCC 3 cut(s) 198, 215, 429
BspCNI CTCAG 2 cut(s) 142, 154
BspLI GGNNCC 3 cut(s) 128, 399, 489
BspPI GGATC 3 cut(s) 513, 524, 659
BspT107I GGYRCC 1 cut(s) 126
BsrDI GCAATG 2 cut(s) 617, 795
BsrI ACTGG 2 cut(s) 491, 757
BssECI CCNNGG 2 cut(s) 551, 728
BssMI GATC 8 cut(s) 9, 289, 505, 513, 529, 568, 664, 879
BssSI CACGAG 1 cut(s) 118
BssT1I CCWWGG 1 cut(s) 551
Bst2BI CACGAG 1 cut(s) 118
Bst2UI CCWGG 1 cut(s) 336
Bst6I CTCTTC 2 cut(s) 180, 476
BstAPI GCANNNNNTGC 1 cut(s) 772
BstC8I GCNNGC 3 cut(s) 147, 261, 402
BstDEI CTNAG 3 cut(s) 141, 150, 501
BstDSI CCRYGG 1 cut(s) 551
BstF5I GGATG 1 cut(s) 283
BstKTI GATC 8 cut(s) 12, 292, 508, 516, 532, 571, 667, 882
BstMAI GTCTC 1 cut(s) 679
BstMBI GATC 8 cut(s) 9, 289, 505, 513, 529, 568, 664, 879
BstMWI GCNNNNNNNGC 6 cut(s) 91, 146, 151, 260, 410, 772
BstNI CCWGG 1 cut(s) 336
BstSCI CCNGG 2 cut(s) 334, 727
BstV1I GCAGC 2 cut(s) 262, 484
BstX2I RGATCY 2 cut(s) 568, 879
BstXI CCANNNNNNTGG 1 cut(s) 111
BstYI RGATCY 2 cut(s) 568, 879
BsuRI GGCC 3 cut(s) 198, 215, 429
BtgI CCRYGG 1 cut(s) 551
BtsCI GGATG 1 cut(s) 283
BtsI GCAGTG 3 cut(s) 651, 686, 765
BtsIMutI CAGTG 4 cut(s) 453, 651, 686, 765
Cac8I GCNNGC 3 cut(s) 147, 261, 402
CaiI CAGNNNCTG 1 cut(s) 497
Cfr13I GGNCC 2 cut(s) 71, 338
CsiI ACCWGGT 1 cut(s) 334
Csp6I GTAC 1 cut(s) 115
CviAII CATG 6 cut(s) 50, 95, 517, 552, 697, 875
CviQI GTAC 1 cut(s) 115
DdeI CTNAG 3 cut(s) 141, 150, 501
DpnI GATC 8 cut(s) 11, 291, 507, 515, 531, 570, 666, 881
DpnII GATC 8 cut(s) 9, 289, 505, 513, 529, 568, 664, 879
EaeI YGGCCR 2 cut(s) 213, 427
Eam1104I CTCTTC 2 cut(s) 180, 476
EarI CTCTTC 2 cut(s) 180, 476
Eco130I CCWWGG 1 cut(s) 551
Eco47I GGWCC 2 cut(s) 71, 338
Eco57I CTGAAG 2 cut(s) 390, 582
EcoO109I RGGNCCY 1 cut(s) 338
EcoRII CCWGG 1 cut(s) 334
EcoT14I CCWWGG 1 cut(s) 551
EcoT22I ATGCAT 1 cut(s) 586
ErhI CCWWGG 1 cut(s) 551
FaeI CATG 6 cut(s) 53, 98, 520, 555, 700, 878
FalI AAGNNNNNCTT 6 cut(s) 177, 209, 555, 587, 813, 845
FatI CATG 6 cut(s) 49, 94, 516, 551, 696, 874
Fnu4HI GCNGC 3 cut(s) 276, 430, 498
FokI GGATG 1 cut(s) 270
Fsp4HI GCNGC 3 cut(s) 276, 430, 498
FspBI CTAG 2 cut(s) 314, 866
GluI GCNGC 3 cut(s) 276, 430, 498
GsaI CCCAGC 1 cut(s) 408
HaeIII GGCC 3 cut(s) 198, 215, 429
HapII CCGG 1 cut(s) 728
Hin1II CATG 6 cut(s) 53, 98, 520, 555, 700, 878
HindIII AAGCTT 1 cut(s) 44
HinfI GANTC 4 cut(s) 206, 606, 724, 808
HpaII CCGG 1 cut(s) 728
HphI GGTGA 1 cut(s) 713
Hpy166II GTNNAC 1 cut(s) 649
Hpy188I TCNGA 2 cut(s) 562, 884
Hpy188III TCNNGA 6 cut(s) 104, 287, 368, 509, 603, 833
Hpy8I GTNNAC 1 cut(s) 649
HpyAV CCTTC 2 cut(s) 365, 797
HpyCH4IV ACGT 1 cut(s) 714
HpyCH4V TGCA 6 cut(s) 94, 263, 584, 622, 800, 820
HpyF10VI GCNNNNNNNGC 6 cut(s) 91, 146, 151, 260, 410, 772
HpyF3I CTNAG 3 cut(s) 141, 150, 501
HpySE526I ACGT 1 cut(s) 714
Hsp92II CATG 6 cut(s) 53, 98, 520, 555, 700, 878
Kzo9I GATC 8 cut(s) 9, 289, 505, 513, 529, 568, 664, 879
LmnI GCTCC 3 cut(s) 251, 397, 685
Lsp1109I GCAGC 2 cut(s) 262, 484
LweI GCATC 1 cut(s) 593
MabI ACCWGGT 1 cut(s) 334
MaeI CTAG 2 cut(s) 314, 866
MaeII ACGT 1 cut(s) 714
MalI GATC 8 cut(s) 11, 291, 507, 515, 531, 570, 666, 881
MboI GATC 8 cut(s) 9, 289, 505, 513, 529, 568, 664, 879
MboII GAAGA 6 cut(s) 197, 353, 356, 463, 575, 578
MflI RGATCY 2 cut(s) 568, 879
MhlI GDGCHC 1 cut(s) 18
MlsI TGGCCA 1 cut(s) 215
MluCI AATT 5 cut(s) 60, 624, 767, 840, 852
MluNI TGGCCA 1 cut(s) 215
MmeI TCCRAC 1 cut(s) 707
MnlI CCTC 6 cut(s) 29, 117, 181, 402, 478, 552
Mox20I TGGCCA 1 cut(s) 215
Mph1103I ATGCAT 1 cut(s) 586
MroXI GAANNNNTTC 3 cut(s) 189, 812, 828
MscI TGGCCA 1 cut(s) 215
MseI TTAA 5 cut(s) 219, 441, 719, 789, 843
MslI CAYNNNNRTG 1 cut(s) 21
Msp20I TGGCCA 1 cut(s) 215
MspA1I CMGCKG 2 cut(s) 149, 404
MspI CCGG 1 cut(s) 728
MspR9I CCNGG 2 cut(s) 336, 729
MvaI CCWGG 1 cut(s) 336
MwoI GCNNNNNNNGC 6 cut(s) 91, 146, 151, 260, 410, 772
NciI CCSGG 1 cut(s) 729
NcoI CCATGG 1 cut(s) 551
NdeII GATC 8 cut(s) 9, 289, 505, 513, 529, 568, 664, 879
NlaIII CATG 6 cut(s) 53, 98, 520, 555, 700, 878
NlaIV GGNNCC 3 cut(s) 128, 399, 489
NsiI ATGCAT 1 cut(s) 586
PdmI GAANNNNTTC 3 cut(s) 189, 812, 828
PfeI GAWTC 4 cut(s) 206, 606, 724, 808
PflFI GACNNNGTC 1 cut(s) 713
PkrI GCNGC 3 cut(s) 277, 431, 499
PpuMI RGGWCCY 1 cut(s) 338
PshBI ATTAAT 1 cut(s) 843
Psp5II RGGWCCY 1 cut(s) 338
Psp6I CCWGG 1 cut(s) 334
PspFI CCCAGC 1 cut(s) 404
PspGI CCWGG 1 cut(s) 334
PspN4I GGNNCC 3 cut(s) 128, 399, 489
PspPI GGNCC 2 cut(s) 71, 338
PspPPI RGGWCCY 1 cut(s) 338
PstNI CAGNNNCTG 1 cut(s) 497
PsuI RGATCY 2 cut(s) 568, 879
PsyI GACNNNGTC 1 cut(s) 713
PvuII CAGCTG 2 cut(s) 149, 404
RsaI GTAC 1 cut(s) 116
RsaNI GTAC 1 cut(s) 115
RseI CAYNNNNRTG 1 cut(s) 21
SaqAI TTAA 5 cut(s) 219, 441, 719, 789, 843
SatI GCNGC 3 cut(s) 276, 430, 498
Sau3AI GATC 8 cut(s) 9, 289, 505, 513, 529, 568, 664, 879
Sau96I GGNCC 2 cut(s) 71, 338
ScrFI CCNGG 2 cut(s) 336, 729
SduI GDGCHC 1 cut(s) 18
SexAI ACCWGGT 1 cut(s) 334
SfaNI GCATC 1 cut(s) 593
SinI GGWCC 2 cut(s) 71, 338
SmiMI CAYNNNNRTG 1 cut(s) 21
Sse9I AATT 5 cut(s) 60, 624, 767, 840, 852
SsiI CCGC 2 cut(s) 430, 654
SspMI CTAG 2 cut(s) 314, 866
StyD4I CCNGG 2 cut(s) 334, 727
StyI CCWWGG 1 cut(s) 551
TaiI ACGT 1 cut(s) 717
TaqI TCGA 2 cut(s) 12, 292
TasI AATT 5 cut(s) 60, 624, 767, 840, 852
TauI GCSGC 1 cut(s) 432
TfiI GAWTC 4 cut(s) 206, 606, 724, 808
Tru1I TTAA 5 cut(s) 219, 441, 719, 789, 843
Tru9I TTAA 5 cut(s) 219, 441, 719, 789, 843
TscAI CASTG 4 cut(s) 453, 651, 693, 765
TseI GCWGC 2 cut(s) 275, 497
TspDTI ATGAA 1 cut(s) 38
TspGWI ACGGA 1 cut(s) 599
TspRI CASTG 4 cut(s) 453, 651, 693, 765
Tth111I GACNNNGTC 1 cut(s) 713
VpaK11BI GGWCC 2 cut(s) 71, 338
VspI ATTAAT 1 cut(s) 843
XapI RAATTY 1 cut(s) 624
XmnI GAANNNNTTC 3 cut(s) 189, 812, 828
XspI CTAG 2 cut(s) 314, 866
Zsp2I ATGCAT 1 cut(s) 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.