Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0001414

Physical Location & Seq

Genomic Coordinates Reverse (-)

9779878 .. 9784622

Gene Structure 4745 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1070500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1704 bp

ATGTTTGGCGGTCATGTATTCAGAATTTTGGTTTCAGCCCCATACATTTTTTACAGAAGAATATCAAGATTTGATTTCCTTAATCAAAACTTAACACAGCTGGATGAGAACTCTGAGGATGAGAGGAAAACAAAGCTTATGTCTTTCAAGAAGGCAGCAAACATGATTACAAACTCCTTCAGTAAGGGTCGAAGGAACAGTAAAGTAATGTCTTTCCCTATAGACGATGGCATTGATGCCGAGGAGCTCAAGGCAGCTCAGGAATTCCGGCAAATACTTGTATCCGAAGAACTACTGCCCTCGAAACATGACGATTCTCATATGTTGCTAAGATTTTTAAGGGCCCGGAAATTTGATGTTGAGAAAACAAAGCAAATGTGGTCTGACATGCTCCAGTGGAGGCAGGAATTTGGTGCTGACACTATTATGGAGGACTTCGAATTTAAGGAAGTCGATGAAGTCTTAAAATACTATCCACAAGGGCATCATGGAGTTGATAAGGATGGGCGGCCTGTCTACATTGAGAGACTGGGTCAAGTTGATTCTAATAAGCTGCTGCAAGTGACAACAATGGAACGGTATATAAAGTACCACATACGGGAGTTTGAGAGGACCTTTATCTGTAAATTCCCCGCCTGTTCCGTTGCAGCAAAGAAGCACATTGATCAGAGTACCACTATTCTGGATGTCCAAGGAGTGGGGCTTACAAGTTTAAATAAATCTGCAAGGGAACTCATTCAGCGCCTTCAGAAGGTCGATGGTGACAATTATCCTGAGACCCTGAACCGTATGTTCATCATCAATGCTGGTTCTGGATTTAGGCTCTTATGGAACACCGTCAAATCTTTCCTTGATCCCAAGACCACTGCAAAAATCAATGTTCTGGGAAATCAATATCAGAGCAAGTTGCTTGAAATAATTGAAGCTACTGAGCTACCAGAGTTTTTGGGTGGTAGTTGCACTTGTGCCGATAAGGGTAGCTGCATGATCTCTGATAAGGGCCCGTGGAACGATCCAGAAATAATGAAGATGGTTCAGAGTATTCGTGAGGCCAAATGCACGAGCAACACTTCAGGCGATCTGGAGAAGAAAGAGGAGAAACAGGAGAATCAGGAGAAACAGGAGAAACAGGAAGAGAAAATCTCTGAGGAGAAGAAAATCTCTGAAGAGGAGAAGAAAATCTCTGAAGAGGAGAAGAAAATCTCTGCAGAGGAGAAGAAAGTCTCTGAAGAGGAGAAGTTAATCTCCGAAAATGAGAAGGCTTGCACAAACAGAATGGATTCTTTCTGCTCAGATACGTTTTTAGAGGCTGAAATGCATGGGGACTATCTCTCTTCTGTGCCTGAAGAAGTAAGCATGGCCCTTTTATCTTTTTCGCATACTTATCAGTTACTTTCAATTCCGAGAATGTATCCAATGGGATATGACGATGTTGATCCAATGGTTAGCAAGGGAACTGAACGAGAGGCAGAAAGGCTGAATGCTGCTCTGAATCGACTCGGTGCCTTGGAACAAGAGCTATCTTCAACCAAGCAAGTTGAGACGCAGAAAGAAGAAAAGCTAAAGGCTGCTCTTGACCGCGTCAATGCTTTGGAACAAGAGCTGACAGAAGCCAAGAAGGCACTTGAGGAAGCCCGCCATAAACAAGAGGAGCTTTTAGCCTCCGTTGACAAGGAAAAGAAATCTGGCTTATTCTGTTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000226 GO:0003006 GO:0003674 GO:0005215 GO:0005319 GO:0005548 GO:0005575 GO:0005622 GO:0005623 GO:0005628 GO:0005634 GO:0006629 GO:0006644 GO:0006650 GO:0006656 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007275 GO:0008150 GO:0008152 GO:0008525 GO:0008526 GO:0008610 GO:0008654 GO:0009058 GO:0009653 GO:0009791 GO:0009908 GO:0009987 GO:0010876 GO:0010927 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016192 GO:0019637 GO:0019953 GO:0022402 GO:0022413 GO:0022414 GO:0022607 GO:0030154 GO:0030427 GO:0030435 GO:0030437 GO:0031321 GO:0031322 GO:0032153 GO:0032501 GO:0032502 GO:0032505 GO:0032989 GO:0033036 GO:0034293 GO:0035838 GO:0042763 GO:0042764 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043934 GO:0043935 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044424 GO:0044464 GO:0044703 GO:0045017 GO:0046470 GO:0046474 GO:0046486 GO:0048193 GO:0048367 GO:0048468 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0048869 GO:0051179 GO:0051234 GO:0051286 GO:0051321 GO:0051704 GO:0060187 GO:0061024 GO:0061458 GO:0071702 GO:0071704 GO:0071840 GO:0090407 GO:0090567 GO:0097164 GO:0120009 GO:0120010 GO:1901564 GO:1901566 GO:1901576 GO:1903046

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CRAL_TRIO CRAL_TRIO_N

Protein Analysis

568

Amino Acids

65.37

Weight (kDa)

5.37

Isoelectric Point (pI)

52.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CRAL_TRIO_N	PF03765	88 - 128	1.7e-08	CRAL/TRIO, N-terminal domain
CRAL_TRIO	PF00650	156 - 318	2.4e-29	CRAL/TRIO domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001414)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16380 AT2G16380 AT2G16380 AT2G21540 AT2G21540 AT2G21540 AT2G21540 AT2G21540 AT2G21540 AT4G34580 AT4G34580 AT4G39180 AT4G39180 AT4G39180
fragaria_vesca	FvH4_2g09420 FvH4_2g09420 FvH4_2g09420 FvH4_2g09490 FvH4_2g09490
malus_domestica	MD05G1062700.v1.1 MD05G1062800.v1.1 MD05G1062900.v1.1 MD10G1070400.v1.1 MD10G1070500.v1.1
prunus_persica	Prupe.8G092000_v2.0.a1 Prupe.8G092100_v2.0.a1 Prupe.8G092200_v2.0.a1
pyrus_communis	pycom05g05440 pycom10g05620 pycom10g05630
rosa_chinensis	RchiOBHm_Chr6g0265701 RchiOBHm_Chr6g0265771
rosa_laevigata	RLG00000014161 RLG00000014165
rosa_multiflora	Rmu_co8114364.1_g000001 Rmu_sc0000208.1_g000028 Rmu_sc0008535.1_g000015
rosa_roxburghii	Rroxscaffold_175G00432510 Rroxscaffold_175G00432560 Rroxscaffold_7G00202430
rosa_rugosa	Rorug06G0018500 Rorug06G0019000
rosa_samantha	Rh6AG140700 Rh6AG141500 Rh6BG141100 Rh6BG141400 Rh6CG137700 Rh6CG138000 Rh6DG124600 Rh6DG125000
rosa_wichuraiana	Rw6G012170 Rw6G012230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1502
AccB7I	CCANNNNNTGG	1 cut(s)	697
AccI	GTMKAC	1 cut(s)	516
AccII	CGCG	1 cut(s)	1581
AciI	CCGC	5 cut(s)	9, 508, 633, 1579, 1636
AclWI	GGATC	3 cut(s)	848, 1007, 1430
AcsI	RAATTY	6 cut(s)	24, 263, 350, 407, 440, 626
AcuI	CTGAAG	7 cut(s)	163, 731, 1056, 1185, 1206, 1248, 1365
AfaI	GTAC	2 cut(s)	590, 673
AfiI	CCNNNNNNNGG	3 cut(s)	598, 697, 751
AgsI	TTSAA	5 cut(s)	148, 914, 923, 1398, 1527
Alw21I	GWGCWC	1 cut(s)	249
Alw26I	GTCTC	4 cut(s)	520, 770, 1228, 1535
AlwI	GGATC	3 cut(s)	848, 1007, 1430
AoxI	GGCC	5 cut(s)	342, 509, 1000, 1050, 1359
ApaI	GGGCCC	2 cut(s)	346, 1004
ApeKI	GCWGC	8 cut(s)	155, 254, 553, 556, 647, 981, 1484, 1568
ApoI	RAATTY	6 cut(s)	24, 263, 350, 407, 440, 626
ArsI	GACNNNNNNTTYG	2 cut(s)	125, 157
Asp700I	GAANNNNTTC	2 cut(s)	735, 1279
AspLEI	GCGC	1 cut(s)	744
AspS9I	GGNCC	6 cut(s)	342, 343, 612, 1000, 1001, 1360
AsuC2I	CCSGG	1 cut(s)	346
AsuHPI	GGTGA	1 cut(s)	773
AsuII	TTCGAA	1 cut(s)	438
AvaII	GGWCC	1 cut(s)	612
BaeGI	GKGCMC	2 cut(s)	346, 1004
BanI	GGYRCC	1 cut(s)	1502
BanII	GRGCYC	3 cut(s)	249, 346, 1004
BauI	CACGAG	1 cut(s)	1060
Bbv12I	GWGCWC	1 cut(s)	249
BbvI	GCAGC	8 cut(s)	167, 266, 540, 543, 659, 968, 1471, 1555
BccI	CCATC	4 cut(s)	221, 497, 752, 1024
BciVI	GTATCC	2 cut(s)	292, 1422
BclI	TGATCA	1 cut(s)	664
BcnI	CCSGG	1 cut(s)	346
BcoDI	GTCTC	4 cut(s)	520, 770, 1228, 1535
BfmI	CTRYAG	2 cut(s)	219, 1206
BfoI	RGCGCY	1 cut(s)	745
BfuI	GTATCC	2 cut(s)	292, 1422
BglI	GCCNNNNNGGC	1 cut(s)	1619
BisI	GCNGC	9 cut(s)	156, 255, 509, 554, 557, 648, 982, 1485, 1569
BlsI	GCNGC	9 cut(s)	157, 256, 510, 555, 558, 649, 983, 1486, 1570
Bme1390I	CCNGG	1 cut(s)	346
Bme18I	GGWCC	1 cut(s)	612
BmgT120I	GGNCC	6 cut(s)	342, 343, 612, 1000, 1001, 1360
BmiI	GGNNCC	3 cut(s)	344, 1002, 1504
BmrFI	CCNGG	1 cut(s)	346
BmrI	ACTGGG	1 cut(s)	539
BmsI	GCATC	2 cut(s)	226, 493
BmuI	ACTGGG	1 cut(s)	539
BplI	GAGNNNNNCTC	2 cut(s)	1127, 1159
BpmI	CTGGAG	2 cut(s)	377, 1103
Bpu10I	CCTNAGC	1 cut(s)	258
Bpu14I	TTCGAA	1 cut(s)	438
BpuEI	CTTGAG	2 cut(s)	233, 1646
BpuMI	CCSGG	1 cut(s)	346
BsaBI	GATNNNNATC	1 cut(s)	1434
BsaI	GGTCTC	1 cut(s)	770
BsaJI	CCNNGG	4 cut(s)	240, 691, 1004, 1506
Bsc4I	CCNNNNNNNGG	3 cut(s)	598, 697, 751
Bse1I	ACTGG	2 cut(s)	394, 534
Bse8I	GATNNNNATC	1 cut(s)	1434
BseDI	CCNNGG	4 cut(s)	240, 691, 1004, 1506
BseGI	GGATG	4 cut(s)	109, 124, 508, 691
BseJI	GATNNNNATC	1 cut(s)	1434
BseLI	CCNNNNNNNGG	3 cut(s)	598, 697, 751
BseMII	CTCAG	6 cut(s)	105, 272, 765, 921, 1137, 1305
BseNI	ACTGG	2 cut(s)	394, 534
BseRI	GAGGAG	8 cut(s)	257, 1109, 1163, 1184, 1205, 1226, 1247, 1664
BseSI	GKGCMC	2 cut(s)	346, 1004
BseXI	GCAGC	8 cut(s)	167, 266, 540, 543, 659, 968, 1471, 1555
Bsh1236I	CGCG	1 cut(s)	1581
BshFI	GGCC	5 cut(s)	344, 511, 1002, 1052, 1361
BshNI	GGYRCC	1 cut(s)	1502
BsiHKAI	GWGCWC	1 cut(s)	249
BsiSI	CCGG	2 cut(s)	268, 346
BslFI	GGGAC	1 cut(s)	1337
BslI	CCNNNNNNNGG	3 cut(s)	598, 697, 751
BsmAI	GTCTC	4 cut(s)	520, 770, 1228, 1535
BsmBI	CGTCTC	1 cut(s)	1535
BsmFI	GGGAC	1 cut(s)	1337
BsmI	GAATGC	1 cut(s)	1486
BsnI	GGCC	5 cut(s)	344, 511, 1002, 1052, 1361
Bso31I	GGTCTC	1 cut(s)	770
Bsp119I	TTCGAA	1 cut(s)	438
Bsp120I	GGGCCC	2 cut(s)	342, 1000
Bsp1286I	GDGCHC	3 cut(s)	249, 346, 1004
Bsp143I	GATC	6 cut(s)	664, 853, 987, 1012, 1078, 1435
BspACI	CCGC	5 cut(s)	9, 508, 633, 1579, 1636
BspANI	GGCC	5 cut(s)	344, 511, 1002, 1052, 1361
BspCNI	CTCAG	6 cut(s)	106, 271, 766, 922, 1138, 1304
BspFNI	CGCG	1 cut(s)	1581
BspLI	GGNNCC	3 cut(s)	344, 1002, 1504
BspMAI	CTGCAG	1 cut(s)	1210
BspPI	GGATC	3 cut(s)	848, 1007, 1430
BspT104I	TTCGAA	1 cut(s)	438
BspT107I	GGYRCC	1 cut(s)	1502
BspTNI	GGTCTC	1 cut(s)	770
BsrI	ACTGG	2 cut(s)	394, 534
BssECI	CCNNGG	4 cut(s)	240, 691, 1004, 1506
BssMI	GATC	6 cut(s)	664, 853, 987, 1012, 1078, 1435
BssSI	CACGAG	1 cut(s)	1060
BssT1I	CCWWGG	2 cut(s)	691, 1506
Bst2BI	CACGAG	1 cut(s)	1060
Bst4CI	ACNGT	4 cut(s)	200, 579, 788, 838
Bst6I	CTCTTC	5 cut(s)	1128, 1161, 1182, 1224, 1339
BstBI	TTCGAA	1 cut(s)	438
BstC8I	GCNNGC	2 cut(s)	1264, 1636
BstDEI	CTNAG	7 cut(s)	114, 258, 329, 774, 930, 1146, 1291
BstDSI	CCRYGG	1 cut(s)	1004
BstENI	CCTNNNNNAGG	1 cut(s)	749
BstF5I	GGATG	4 cut(s)	109, 124, 508, 691
BstFNI	CGCG	1 cut(s)	1581
BstH2I	RGCGCY	1 cut(s)	745
BstHHI	GCGC	1 cut(s)	744
BstKTI	GATC	6 cut(s)	667, 856, 990, 1015, 1081, 1438
BstMAI	GTCTC	4 cut(s)	520, 770, 1228, 1535
BstMBI	GATC	6 cut(s)	664, 853, 987, 1012, 1078, 1435
BstMWI	GCNNNNNNNGC	1 cut(s)	1619
BstNSI	RCATGY	1 cut(s)	391
BstSCI	CCNGG	1 cut(s)	344
BstSFI	CTRYAG	2 cut(s)	219, 1206
BstSLI	GKGCMC	2 cut(s)	346, 1004
BstUI	CGCG	1 cut(s)	1581
BstV1I	GCAGC	8 cut(s)	167, 266, 540, 543, 659, 968, 1471, 1555
BstXI	CCANNNNNNTGG	1 cut(s)	682
BsuI	GTATCC	2 cut(s)	292, 1422
BsuRI	GGCC	5 cut(s)	344, 511, 1002, 1052, 1361
BtgI	CCRYGG	1 cut(s)	1004
BtsCI	GGATG	4 cut(s)	109, 124, 508, 691
BtsI	GCAGTG	1 cut(s)	864
BtsIMutI	CAGTG	2 cut(s)	401, 864
Cac8I	GCNNGC	2 cut(s)	1264, 1636
CfoI	GCGC	1 cut(s)	744
Cfr13I	GGNCC	6 cut(s)	342, 343, 612, 1000, 1001, 1360
CseI	GACGC	2 cut(s)	1552, 1570
Csp6I	GTAC	2 cut(s)	589, 672
CviAII	CATG	8 cut(s)	14, 163, 308, 388, 488, 985, 1319, 1357
CviQI	GTAC	2 cut(s)	589, 672
DdeI	CTNAG	7 cut(s)	114, 258, 329, 774, 930, 1146, 1291
DpnI	GATC	6 cut(s)	666, 855, 989, 1014, 1080, 1437
DpnII	GATC	6 cut(s)	664, 853, 987, 1012, 1078, 1435
DraI	TTTAAA	1 cut(s)	714
Eam1104I	CTCTTC	5 cut(s)	1128, 1161, 1182, 1224, 1339
EarI	CTCTTC	5 cut(s)	1128, 1161, 1182, 1224, 1339
Ecl136II	GAGCTC	1 cut(s)	247
Eco130I	CCWWGG	2 cut(s)	691, 1506
Eco24I	GRGCYC	3 cut(s)	249, 346, 1004
Eco31I	GGTCTC	1 cut(s)	770
Eco47I	GGWCC	1 cut(s)	612
Eco53kI	GAGCTC	1 cut(s)	247
Eco57I	CTGAAG	7 cut(s)	163, 731, 1056, 1185, 1206, 1248, 1365
EcoICRI	GAGCTC	1 cut(s)	247
EcoNI	CCTNNNNNAGG	1 cut(s)	749
EcoO109I	RGGNCCY	3 cut(s)	342, 612, 1000
EcoRI	GAATTC	1 cut(s)	263
EcoT14I	CCWWGG	2 cut(s)	691, 1506
EcoT22I	ATGCAT	1 cut(s)	1320
EcoT38I	GRGCYC	3 cut(s)	249, 346, 1004
ErhI	CCWWGG	2 cut(s)	691, 1506
Esp3I	CGTCTC	1 cut(s)	1535
FaeI	CATG	8 cut(s)	17, 166, 311, 391, 491, 988, 1322, 1360
FalI	AAGNNNNNCTT	2 cut(s)	1638, 1670
FaqI	GGGAC	1 cut(s)	1337
FatI	CATG	8 cut(s)	13, 162, 307, 387, 487, 984, 1318, 1356
FauI	CCCGC	2 cut(s)	640, 1643
FauNDI	CATATG	1 cut(s)	321
FbaI	TGATCA	1 cut(s)	664
FblI	GTMKAC	1 cut(s)	516
Fnu4HI	GCNGC	9 cut(s)	156, 255, 509, 554, 557, 648, 982, 1485, 1569
FokI	GGATG	4 cut(s)	116, 131, 515, 698
FriOI	GRGCYC	3 cut(s)	249, 346, 1004
Fsp4HI	GCNGC	9 cut(s)	156, 255, 509, 554, 557, 648, 982, 1485, 1569
GlaI	GCGC	1 cut(s)	743
GluI	GCNGC	9 cut(s)	156, 255, 509, 554, 557, 648, 982, 1485, 1569
GsuI	CTGGAG	2 cut(s)	377, 1103
HaeII	RGCGCY	1 cut(s)	745
HaeIII	GGCC	5 cut(s)	344, 511, 1002, 1052, 1361
HapII	CCGG	2 cut(s)	268, 346
HgaI	GACGC	2 cut(s)	1552, 1570
HhaI	GCGC	1 cut(s)	744
Hin1II	CATG	8 cut(s)	17, 166, 311, 391, 491, 988, 1322, 1360
Hin6I	GCGC	1 cut(s)	742
HinP1I	GCGC	1 cut(s)	742
HincII	GTYRAC	1 cut(s)	1669
HindII	GTYRAC	1 cut(s)	1669
HindIII	AAGCTT	1 cut(s)	134
HinfI	GANTC	6 cut(s)	314, 542, 1108, 1280, 1492, 1497
HpaII	CCGG	2 cut(s)	268, 346
HphI	GGTGA	1 cut(s)	773
Hpy166II	GTNNAC	2 cut(s)	517, 1669
Hpy8I	GTNNAC	2 cut(s)	517, 1669
HpyAV	CCTTC	7 cut(s)	145, 186, 187, 745, 755, 1252, 1612
HpyCH4III	ACNGT	4 cut(s)	200, 579, 788, 838
HpyCH4IV	ACGT	1 cut(s)	1298
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1619
HpyF3I	CTNAG	7 cut(s)	114, 258, 329, 774, 930, 1146, 1291
HpySE526I	ACGT	1 cut(s)	1298
Hsp92II	CATG	8 cut(s)	17, 166, 311, 391, 491, 988, 1322, 1360
HspAI	GCGC	1 cut(s)	742
Ksp22I	TGATCA	1 cut(s)	664
Kzo9I	GATC	6 cut(s)	664, 853, 987, 1012, 1078, 1435
LmnI	GCTCC	3 cut(s)	244, 396, 1651
Lsp1109I	GCAGC	8 cut(s)	167, 266, 540, 543, 659, 968, 1471, 1555
LweI	GCATC	2 cut(s)	226, 493
MaeII	ACGT	1 cut(s)	1298
MaeIII	GTNAC	3 cut(s)	562, 761, 1389
MalI	GATC	6 cut(s)	666, 855, 989, 1014, 1080, 1437
MboI	GATC	6 cut(s)	664, 853, 987, 1012, 1078, 1435
MhlI	GDGCHC	3 cut(s)	249, 346, 1004
MluCI	AATT	9 cut(s)	24, 263, 350, 407, 440, 626, 766, 918, 1398
MlyI	GAGTC	1 cut(s)	1491
Mph1103I	ATGCAT	1 cut(s)	1320
MroXI	GAANNNNTTC	2 cut(s)	735, 1279
MseI	TTAA	7 cut(s)	81, 92, 338, 444, 464, 713, 1241
MslI	CAYNNNNRTG	1 cut(s)	425
MspA1I	CMGCKG	1 cut(s)	100
MspI	CCGG	2 cut(s)	268, 346
MspR9I	CCNGG	1 cut(s)	346
Mva1269I	GAATGC	1 cut(s)	1486
MvnI	CGCG	1 cut(s)	1581
MwoI	GCNNNNNNNGC	1 cut(s)	1619
NciI	CCSGG	1 cut(s)	346
NdeI	CATATG	1 cut(s)	321
NdeII	GATC	6 cut(s)	664, 853, 987, 1012, 1078, 1435
NlaIII	CATG	8 cut(s)	17, 166, 311, 391, 491, 988, 1322, 1360
NlaIV	GGNNCC	3 cut(s)	344, 1002, 1504
NmeAIII	GCCGAG	1 cut(s)	265
NmuCI	GTSAC	2 cut(s)	562, 761
NsiI	ATGCAT	1 cut(s)	1320
NspI	RCATGY	1 cut(s)	391
NspV	TTCGAA	1 cut(s)	438
PctI	GAATGC	1 cut(s)	1486
PdmI	GAANNNNTTC	2 cut(s)	735, 1279
PfeI	GAWTC	5 cut(s)	314, 542, 1108, 1280, 1492
PflFI	GACNNNGTC	2 cut(s)	531, 1580
PflMI	CCANNNNNTGG	1 cut(s)	697
PkrI	GCNGC	9 cut(s)	157, 256, 510, 555, 558, 649, 983, 1486, 1570
PleI	GAGTC	1 cut(s)	1491
PpsI	GAGTC	1 cut(s)	1491
PpuMI	RGGWCCY	1 cut(s)	612
Psp124BI	GAGCTC	1 cut(s)	249
Psp5II	RGGWCCY	1 cut(s)	612
PspN4I	GGNNCC	3 cut(s)	344, 1002, 1504
PspOMI	GGGCCC	2 cut(s)	342, 1000
PspPI	GGNCC	6 cut(s)	342, 343, 612, 1000, 1001, 1360
PspPPI	RGGWCCY	1 cut(s)	612
PstI	CTGCAG	1 cut(s)	1210
PsyI	GACNNNGTC	2 cut(s)	531, 1580
PvuII	CAGCTG	1 cut(s)	100
RsaI	GTAC	2 cut(s)	590, 673
RsaNI	GTAC	2 cut(s)	589, 672
RseI	CAYNNNNRTG	1 cut(s)	425
SacI	GAGCTC	1 cut(s)	249
SaqAI	TTAA	7 cut(s)	81, 92, 338, 444, 464, 713, 1241
SatI	GCNGC	9 cut(s)	156, 255, 509, 554, 557, 648, 982, 1485, 1569
Sau3AI	GATC	6 cut(s)	664, 853, 987, 1012, 1078, 1435
Sau96I	GGNCC	6 cut(s)	342, 343, 612, 1000, 1001, 1360
SchI	GAGTC	1 cut(s)	1491
ScrFI	CCNGG	1 cut(s)	346
SduI	GDGCHC	3 cut(s)	249, 346, 1004
SfaNI	GCATC	2 cut(s)	226, 493
SfcI	CTRYAG	2 cut(s)	219, 1206
SfuI	TTCGAA	1 cut(s)	438
SinI	GGWCC	1 cut(s)	612
SmiMI	CAYNNNNRTG	1 cut(s)	425
SmlI	CTYRAG	2 cut(s)	248, 1625
SmoI	CTYRAG	2 cut(s)	248, 1625
Sse9I	AATT	9 cut(s)	24, 263, 350, 407, 440, 626, 766, 918, 1398
SsiI	CCGC	5 cut(s)	9, 508, 633, 1579, 1636
SstI	GAGCTC	1 cut(s)	249
StyD4I	CCNGG	1 cut(s)	344
StyI	CCWWGG	2 cut(s)	691, 1506
TaaI	ACNGT	4 cut(s)	200, 579, 788, 838
TaiI	ACGT	1 cut(s)	1301
TaqI	TCGA	6 cut(s)	190, 302, 438, 453, 756, 1495
TasI	AATT	9 cut(s)	24, 263, 350, 407, 440, 626, 766, 918, 1398
TauI	GCSGC	1 cut(s)	511
TfiI	GAWTC	5 cut(s)	314, 542, 1108, 1280, 1492
Tru1I	TTAA	7 cut(s)	81, 92, 338, 444, 464, 713, 1241
Tru9I	TTAA	7 cut(s)	81, 92, 338, 444, 464, 713, 1241
TscAI	CASTG	2 cut(s)	401, 871
TseFI	GTSAC	2 cut(s)	562, 761
TseI	GCWGC	8 cut(s)	155, 254, 553, 556, 647, 981, 1484, 1568
Tsp45I	GTSAC	2 cut(s)	562, 761
TspDTI	ATGAA	3 cut(s)	471, 784, 1040
TspGWI	ACGGA	2 cut(s)	631, 1654
TspRI	CASTG	2 cut(s)	401, 871
Tth111I	GACNNNGTC	2 cut(s)	531, 1580
Van91I	CCANNNNNTGG	1 cut(s)	697
VpaK11BI	GGWCC	1 cut(s)	612
XagI	CCTNNNNNAGG	1 cut(s)	749
XapI	RAATTY	6 cut(s)	24, 263, 350, 407, 440, 626
XceI	RCATGY	1 cut(s)	391
XmiI	GTMKAC	1 cut(s)	516
XmnI	GAANNNNTTC	2 cut(s)	735, 1279
Zsp2I	ATGCAT	1 cut(s)	1320

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.