Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0001414

Physical Location & Seq

Genomic Coordinates Reverse (-)

48346413 .. 48350131

Gene Structure 3719 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000014161

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1323 bp

ATGTCTGGACCTCTTGACCGACACCTAAAACCTGGTAAGTTCATTAATAAATCCCATGAATTTGAATACAAGGAAATTGATGAGGTTTTGAAATACTATCCCCAAGGCCATCATGGAGTTGATAAGGAGGGAAGACCAGTCTACATTGAGAGACTTGGTCAAGTGGATTCAACCAAGCTGATGCAAGTCACAACAATGGACCGTTATGTTAAATACCATGTACGGGAGTTTGAGAGGACCTTCGTCCATAAATTCCCTGCATGTTCCATTGCAGCAAAGAAGCACATTGATCAGAGCACCACTATTCTTGATGTACAAGGAGTGGGGCTTAAAAATTTCAATAAATCTGCGAGGGAACTTATTCAGTGCCTTCAGAAGATTGATGGCGACAATTATCCTGAGGTTCTTGGAAACAAATACCAGAGCAAGTTGCTTGAAATAATTGATGCTAGTGAGCTGCCAGAGTTTTTGGGTGGCACTTGTACTTGTGCAGATAAGGGTGGTTGCATGCGTTCCGATAAAGGCCCATGGAATGACCCAGATATAATGAAGATGGTTCACAATGGTGAGGGCAAATGCACAAGGAGAACATTATCAGGCATTGAGGAGAAGATAATCTCTGAAGATGAAATGTTTTACTCCAAGACATGTGATTCTTTCAACTCAAAAATAGCTCAAATGGAACATACAAAACTCTCTCATGTTCCTAAACAAGTTTCATTCACCAAAAAATCCAGTAAGGCCTATGAGTATGACAAGTTGATGCCAATGGTTGACAAGACCGTGGATGCAACTTGGCCTAAACCAGTTCATTATGATAAATTTGCCCACAGTCAAGCTACAGATTGCTTCCCCTCGCATAATGCTTGCAAGTCTTCTGAAGGACTAAGCAGTTACTTACTTTCTGGAATCATGGCCTTCATCATGGGAATTGTCACCATGATCCAATTGACACGAGACATGCCAAGGAAACTCACTGAGGCTGCCCTCTATGGAAGCCCTGCTTACTGCAATGGAATGGGGATGAAAGCCAAGCAGTTGCCGGCATCATCCATCTCCAGTGACACATATATGAACATGATGAAGCGCATGGCCGAGTTGGAGGAGAAAGTGAAAGTTCTCAGCACGAAACCAACTGTTATGCCCCCTGAGAAAGAGGAAATGTTGAACAATGCTCTCAACCGAGCCACAGCCTTGGAACAAGAGCTTTCAGCAACCAAGAAGGCGCTTGATGACGCCCTGGTCCGACAGAATGAGCTTATAGCCTACCTTGATAAGAAGAAAAAGAAGAAGAAACTGAAATTGAACCCATTTAAATGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000226 GO:0003006 GO:0003674 GO:0005215 GO:0005319 GO:0005548 GO:0005575 GO:0005622 GO:0005623 GO:0005628 GO:0005634 GO:0006629 GO:0006644 GO:0006650 GO:0006656 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007275 GO:0008150 GO:0008152 GO:0008525 GO:0008526 GO:0008610 GO:0008654 GO:0009058 GO:0009653 GO:0009791 GO:0009908 GO:0009987 GO:0010876 GO:0010927 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016192 GO:0019637 GO:0019953 GO:0022402 GO:0022413 GO:0022414 GO:0022607 GO:0030154 GO:0030427 GO:0030435 GO:0030437 GO:0031321 GO:0031322 GO:0032153 GO:0032501 GO:0032502 GO:0032505 GO:0032989 GO:0033036 GO:0034293 GO:0035838 GO:0042763 GO:0042764 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043934 GO:0043935 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044424 GO:0044464 GO:0044703 GO:0045017 GO:0046470 GO:0046474 GO:0046486 GO:0048193 GO:0048367 GO:0048468 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0048869 GO:0051179 GO:0051234 GO:0051286 GO:0051321 GO:0051704 GO:0060187 GO:0061024 GO:0061458 GO:0071702 GO:0071704 GO:0071840 GO:0090407 GO:0090567 GO:0097164 GO:0120009 GO:0120010 GO:1901564 GO:1901566 GO:1901576 GO:1903046

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CRAL_TRIO CRAL_TRIO_N

Protein Analysis

441

Amino Acids

49.96

Weight (kDa)

8.87

Isoelectric Point (pI)

44.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CRAL_TRIO	PF00650	31 - 139	1.8e-11	CRAL/TRIO domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001414)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16380 AT2G16380 AT2G16380 AT2G21540 AT2G21540 AT2G21540 AT2G21540 AT2G21540 AT2G21540 AT4G34580 AT4G34580 AT4G39180 AT4G39180 AT4G39180
fragaria_vesca	FvH4_2g09420 FvH4_2g09420 FvH4_2g09420 FvH4_2g09490 FvH4_2g09490
malus_domestica	MD05G1062700.v1.1 MD05G1062800.v1.1 MD05G1062900.v1.1 MD10G1070400.v1.1 MD10G1070500.v1.1
prunus_persica	Prupe.8G092000_v2.0.a1 Prupe.8G092100_v2.0.a1 Prupe.8G092200_v2.0.a1
pyrus_communis	pycom05g05440 pycom10g05620 pycom10g05630
rosa_chinensis	RchiOBHm_Chr6g0265701 RchiOBHm_Chr6g0265771
rosa_laevigata	RLG00000014161 RLG00000014165
rosa_multiflora	Rmu_co8114364.1_g000001 Rmu_sc0000208.1_g000028 Rmu_sc0008535.1_g000015
rosa_roxburghii	Rroxscaffold_175G00432510 Rroxscaffold_175G00432560 Rroxscaffold_7G00202430
rosa_rugosa	Rorug06G0018500 Rorug06G0019000
rosa_samantha	Rh6AG140700 Rh6AG141500 Rh6BG141100 Rh6BG141400 Rh6CG137700 Rh6CG138000 Rh6DG124600 Rh6DG125000
rosa_wichuraiana	Rw6G012170 Rw6G012230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1241
AccI	GTMKAC	1 cut(s)	141
AclWI	GGATC	1 cut(s)	937
AcoI	YGGCCR	1 cut(s)	1092
AcsI	RAATTY	4 cut(s)	59, 251, 334, 821
AcuI	CTGAAG	3 cut(s)	356, 642, 900
AcyI	GRCGYC	1 cut(s)	1236
AfaI	GTAC	3 cut(s)	222, 315, 484
AfiI	CCNNNNNNNGG	1 cut(s)	223
AflIII	ACRYGT	1 cut(s)	647
AgsI	TTSAA	8 cut(s)	65, 91, 171, 340, 437, 661, 1168, 1306
AjnI	CCWGG	2 cut(s)	31, 1239
AleI	CACNNNNGTG	1 cut(s)	564
AluBI	AGCT	6 cut(s)	178, 457, 674, 839, 1207, 1258
AluI	AGCT	6 cut(s)	178, 457, 674, 839, 1207, 1258
Alw21I	GWGCWC	1 cut(s)	299
Alw26I	GTCTC	2 cut(s)	145, 951
AlwI	GGATC	1 cut(s)	937
AoxI	GGCC	6 cut(s)	106, 523, 741, 797, 915, 1092
ApeKI	GCWGC	3 cut(s)	272, 457, 983
ApoI	RAATTY	4 cut(s)	59, 251, 334, 821
AseI	ATTAAT	1 cut(s)	45
Asp700I	GAANNNNTTC	1 cut(s)	360
AspLEI	GCGC	2 cut(s)	1089, 1228
AspS9I	GGNCC	5 cut(s)	8, 199, 237, 524, 1243
AsuHPI	GGTGA	3 cut(s)	578, 715, 928
AvaII	GGWCC	4 cut(s)	8, 199, 237, 1243
AxyI	CCTNAGG	1 cut(s)	399
BauI	CACGAG	1 cut(s)	954
BbsI	GAAGAC	2 cut(s)	139, 867
Bbv12I	GWGCWC	1 cut(s)	299
BbvI	GCAGC	3 cut(s)	284, 444, 970
BccI	CCATC	4 cut(s)	117, 377, 547, 1061
BciT130I	CCWGG	2 cut(s)	33, 1241
BclI	TGATCA	1 cut(s)	289
BcoDI	GTCTC	2 cut(s)	145, 951
BfaI	CTAG	1 cut(s)	450
BfmI	CTRYAG	1 cut(s)	840
BfoI	RGCGCY	1 cut(s)	1229
BisI	GCNGC	3 cut(s)	273, 458, 984
BlsI	GCNGC	3 cut(s)	274, 459, 985
Bme1390I	CCNGG	2 cut(s)	33, 1241
Bme18I	GGWCC	4 cut(s)	8, 199, 237, 1243
BmgT120I	GGNCC	5 cut(s)	8, 199, 237, 524, 1243
BmrFI	CCNGG	2 cut(s)	33, 1241
BmsI	GCATC	5 cut(s)	171, 436, 753, 778, 1055
BoxI	GACNNNNGTC	1 cut(s)	242
BpiI	GAAGAC	2 cut(s)	139, 867
BpmI	CTGGAG	1 cut(s)	1042
BsaHI	GRCGYC	1 cut(s)	1236
BsaJI	CCNNGG	6 cut(s)	103, 527, 783, 965, 1194, 1239
Bsc4I	CCNNNNNNNGG	1 cut(s)	223
Bse118I	RCCGGY	1 cut(s)	1042
Bse1I	ACTGG	4 cut(s)	137, 735, 806, 1059
Bse21I	CCTNAGG	1 cut(s)	399
Bse3DI	GCAATG	2 cut(s)	267, 1018
BseBI	CCWGG	2 cut(s)	33, 1241
BseDI	CCNNGG	6 cut(s)	103, 527, 783, 965, 1194, 1239
BseGI	GGATG	3 cut(s)	793, 1029, 1049
BseLI	CCNNNNNNNGG	1 cut(s)	223
BseMI	GCAATG	2 cut(s)	267, 1018
BseMII	CTCAG	4 cut(s)	390, 969, 1135, 1140
BseNI	ACTGG	4 cut(s)	137, 735, 806, 1059
BseRI	GAGGAG	2 cut(s)	620, 1118
BseXI	GCAGC	3 cut(s)	284, 444, 970
BsgI	GTGCAG	1 cut(s)	510
BshFI	GGCC	6 cut(s)	108, 525, 743, 799, 917, 1094
BsiHKAI	GWGCWC	1 cut(s)	299
BsiSI	CCGG	1 cut(s)	1043
BslI	CCNNNNNNNGG	1 cut(s)	223
BsmAI	GTCTC	2 cut(s)	145, 951
BsnI	GGCC	6 cut(s)	108, 525, 743, 799, 917, 1094
Bsp1286I	GDGCHC	1 cut(s)	299
Bsp1407I	TGTACA	1 cut(s)	313
Bsp143I	GATC	2 cut(s)	289, 942
Bsp19I	CCATGG	1 cut(s)	527
BspANI	GGCC	6 cut(s)	108, 525, 743, 799, 917, 1094
BspCNI	CTCAG	4 cut(s)	391, 970, 1134, 1141
BspPI	GGATC	1 cut(s)	937
BsrDI	GCAATG	2 cut(s)	267, 1018
BsrFI	RCCGGY	1 cut(s)	1042
BsrGI	TGTACA	1 cut(s)	313
BsrI	ACTGG	4 cut(s)	137, 735, 806, 1059
BssAI	RCCGGY	1 cut(s)	1042
BssECI	CCNNGG	6 cut(s)	103, 527, 783, 965, 1194, 1239
BssMI	GATC	2 cut(s)	289, 942
BssNI	GRCGYC	1 cut(s)	1236
BssSI	CACGAG	1 cut(s)	954
BssT1I	CCWWGG	4 cut(s)	103, 527, 965, 1194
Bst2BI	CACGAG	1 cut(s)	954
Bst2UI	CCWGG	2 cut(s)	33, 1241
Bst4CI	ACNGT	4 cut(s)	203, 784, 833, 1138
BstACI	GRCGYC	1 cut(s)	1236
BstAUI	TGTACA	1 cut(s)	313
BstC8I	GCNNGC	3 cut(s)	509, 868, 1044
BstDEI	CTNAG	5 cut(s)	399, 887, 978, 1121, 1149
BstDSI	CCRYGG	2 cut(s)	527, 783
BstF5I	GGATG	3 cut(s)	793, 1029, 1049
BstH2I	RGCGCY	1 cut(s)	1229
BstHHI	GCGC	2 cut(s)	1089, 1228
BstKTI	GATC	2 cut(s)	292, 945
BstMAI	GTCTC	2 cut(s)	145, 951
BstMBI	GATC	2 cut(s)	289, 942
BstNI	CCWGG	2 cut(s)	33, 1241
BstNSI	RCATGY	4 cut(s)	264, 511, 651, 964
BstPAI	GACNNNNGTC	1 cut(s)	242
BstSCI	CCNGG	2 cut(s)	31, 1239
BstSFI	CTRYAG	1 cut(s)	840
BstV1I	GCAGC	3 cut(s)	284, 444, 970
BstV2I	GAAGAC	2 cut(s)	139, 867
BstXI	CCANNNNNNTGG	2 cut(s)	1195, 1317
Bsu36I	CCTNAGG	1 cut(s)	399
BsuRI	GGCC	6 cut(s)	108, 525, 743, 799, 917, 1094
BtgI	CCRYGG	2 cut(s)	527, 783
BtsCI	GGATG	3 cut(s)	793, 1029, 1049
BtsIMutI	CAGTG	3 cut(s)	371, 975, 1066
Cac8I	GCNNGC	3 cut(s)	509, 868, 1044
CfoI	GCGC	2 cut(s)	1089, 1228
Cfr10I	RCCGGY	1 cut(s)	1042
Cfr13I	GGNCC	5 cut(s)	8, 199, 237, 524, 1243
CseI	GACGC	1 cut(s)	1244
CsiI	ACCWGGT	1 cut(s)	31
Csp6I	GTAC	3 cut(s)	221, 314, 483
CspCI	CAANNNNNGTGG	2 cut(s)	289, 324
CviQI	GTAC	3 cut(s)	221, 314, 483
DdeI	CTNAG	5 cut(s)	399, 887, 978, 1121, 1149
DpnI	GATC	2 cut(s)	291, 944
DpnII	GATC	2 cut(s)	289, 942
DraI	TTTAAA	1 cut(s)	1315
DrdI	GACNNNNNNGTC	1 cut(s)	1241
DseDI	GACNNNNNNGTC	1 cut(s)	1241
EaeI	YGGCCR	1 cut(s)	1092
Eco130I	CCWWGG	4 cut(s)	103, 527, 965, 1194
Eco147I	AGGCCT	1 cut(s)	743
Eco47I	GGWCC	4 cut(s)	8, 199, 237, 1243
Eco57I	CTGAAG	3 cut(s)	356, 642, 900
Eco81I	CCTNAGG	1 cut(s)	399
EcoO109I	RGGNCCY	1 cut(s)	237
EcoRII	CCWGG	2 cut(s)	31, 1239
EcoT14I	CCWWGG	4 cut(s)	103, 527, 965, 1194
ErhI	CCWWGG	4 cut(s)	103, 527, 965, 1194
FalI	AAGNNNNNCTT	2 cut(s)	988, 1020
FbaI	TGATCA	1 cut(s)	289
FblI	GTMKAC	1 cut(s)	141
Fnu4HI	GCNGC	3 cut(s)	273, 458, 984
FokI	GGATG	3 cut(s)	800, 1036, 1036
Fsp4HI	GCNGC	3 cut(s)	273, 458, 984
FspBI	CTAG	1 cut(s)	450
GlaI	GCGC	2 cut(s)	1088, 1227
GluI	GCNGC	3 cut(s)	273, 458, 984
GsuI	CTGGAG	1 cut(s)	1042
HaeII	RGCGCY	1 cut(s)	1229
HaeIII	GGCC	6 cut(s)	108, 525, 743, 799, 917, 1094
HapII	CCGG	1 cut(s)	1043
HgaI	GACGC	1 cut(s)	1244
HhaI	GCGC	2 cut(s)	1089, 1228
Hin1I	GRCGYC	1 cut(s)	1236
Hin6I	GCGC	2 cut(s)	1087, 1226
HinP1I	GCGC	2 cut(s)	1087, 1226
HincII	GTYRAC	1 cut(s)	775
HindII	GTYRAC	1 cut(s)	775
HinfI	GANTC	3 cut(s)	167, 653, 909
HpaII	CCGG	1 cut(s)	1043
HphI	GGTGA	3 cut(s)	578, 715, 928
Hpy166II	GTNNAC	3 cut(s)	142, 559, 775
Hpy188I	TCNGA	6 cut(s)	294, 375, 517, 622, 880, 1247
Hpy188III	TCNNGA	5 cut(s)	6, 14, 308, 398, 906
Hpy8I	GTNNAC	3 cut(s)	142, 559, 775
HpyAV	CCTTC	5 cut(s)	250, 380, 875, 928, 1216
HpyCH4III	ACNGT	4 cut(s)	203, 784, 833, 1138
HpyCH4V	TGCA	9 cut(s)	184, 260, 272, 491, 507, 579, 791, 870, 1011
HpyF3I	CTNAG	5 cut(s)	399, 887, 978, 1121, 1149
Hsp92I	GRCGYC	1 cut(s)	1236
HspAI	GCGC	2 cut(s)	1087, 1226
KroI	GCCGGC	1 cut(s)	1042
KroNI	GCCGGC	1 cut(s)	1044
Ksp22I	TGATCA	1 cut(s)	289
Kzo9I	GATC	2 cut(s)	289, 942
Lsp1109I	GCAGC	3 cut(s)	284, 444, 970
LweI	GCATC	5 cut(s)	171, 436, 753, 778, 1055
MabI	ACCWGGT	1 cut(s)	31
MaeI	CTAG	1 cut(s)	450
MaeIII	GTNAC	4 cut(s)	187, 893, 934, 1061
MalI	GATC	2 cut(s)	291, 944
MboI	GATC	2 cut(s)	289, 942
MboII	GAAGA	9 cut(s)	144, 388, 562, 622, 635, 867, 1291, 1300, 1303
MfeI	CAATTG	1 cut(s)	947
MhlI	GDGCHC	1 cut(s)	299
MmeI	TCCRAC	2 cut(s)	1080, 1270
MroNI	GCCGGC	1 cut(s)	1042
MroXI	GAANNNNTTC	1 cut(s)	360
MseI	TTAA	4 cut(s)	45, 210, 330, 1314
MslI	CAYNNNNRTG	4 cut(s)	194, 564, 1070, 1315
MspI	CCGG	1 cut(s)	1043
MspR9I	CCNGG	2 cut(s)	33, 1241
MunI	CAATTG	1 cut(s)	947
MvaI	CCWGG	2 cut(s)	33, 1241
NaeI	GCCGGC	1 cut(s)	1044
NcoI	CCATGG	1 cut(s)	527
NdeII	GATC	2 cut(s)	289, 942
NgoMIV	GCCGGC	1 cut(s)	1042
NmeAIII	GCCGAG	1 cut(s)	1120
NmuCI	GTSAC	3 cut(s)	187, 934, 1061
NspI	RCATGY	4 cut(s)	264, 511, 651, 964
OliI	CACNNNNGTG	1 cut(s)	564
PaeI	GCATGC	1 cut(s)	511
PceI	AGGCCT	1 cut(s)	743
PciI	ACATGT	1 cut(s)	647
PdiI	GCCGGC	1 cut(s)	1044
PdmI	GAANNNNTTC	1 cut(s)	360
PfeI	GAWTC	3 cut(s)	167, 653, 909
PflFI	GACNNNGTC	1 cut(s)	156
PkrI	GCNGC	3 cut(s)	274, 459, 985
PpuMI	RGGWCCY	1 cut(s)	237
PscI	ACATGT	1 cut(s)	647
PshAI	GACNNNNGTC	1 cut(s)	242
PshBI	ATTAAT	1 cut(s)	45
Psp5II	RGGWCCY	1 cut(s)	237
Psp6I	CCWGG	2 cut(s)	31, 1239
PspGI	CCWGG	2 cut(s)	31, 1239
PspPI	GGNCC	5 cut(s)	8, 199, 237, 524, 1243
PspPPI	RGGWCCY	1 cut(s)	237
PsyI	GACNNNGTC	1 cut(s)	156
RsaI	GTAC	3 cut(s)	222, 315, 484
RsaNI	GTAC	3 cut(s)	221, 314, 483
RseI	CAYNNNNRTG	4 cut(s)	194, 564, 1070, 1315
SaqAI	TTAA	4 cut(s)	45, 210, 330, 1314
SatI	GCNGC	3 cut(s)	273, 458, 984
Sau3AI	GATC	2 cut(s)	289, 942
Sau96I	GGNCC	5 cut(s)	8, 199, 237, 524, 1243
ScrFI	CCNGG	2 cut(s)	33, 1241
SduI	GDGCHC	1 cut(s)	299
SexAI	ACCWGGT	1 cut(s)	31
SfaNI	GCATC	5 cut(s)	171, 436, 753, 778, 1055
SfcI	CTRYAG	1 cut(s)	840
SinI	GGWCC	4 cut(s)	8, 199, 237, 1243
SmiI	ATTTAAAT	1 cut(s)	1315
SmiMI	CAYNNNNRTG	4 cut(s)	194, 564, 1070, 1315
SphI	GCATGC	1 cut(s)	511
SseBI	AGGCCT	1 cut(s)	743
SspMI	CTAG	1 cut(s)	450
StuI	AGGCCT	1 cut(s)	743
StyD4I	CCNGG	2 cut(s)	31, 1239
StyI	CCWWGG	4 cut(s)	103, 527, 965, 1194
SwaI	ATTTAAAT	1 cut(s)	1315
TaaI	ACNGT	4 cut(s)	203, 784, 833, 1138
TaqII	GACCGA	1 cut(s)	33
TatI	WGTACW	2 cut(s)	313, 482
TfiI	GAWTC	3 cut(s)	167, 653, 909
Tru1I	TTAA	4 cut(s)	45, 210, 330, 1314
Tru9I	TTAA	4 cut(s)	45, 210, 330, 1314
TscAI	CASTG	3 cut(s)	371, 982, 1066
TseFI	GTSAC	3 cut(s)	187, 934, 1061
TseI	GCWGC	3 cut(s)	272, 457, 983
Tsp45I	GTSAC	3 cut(s)	187, 934, 1061
TspRI	CASTG	3 cut(s)	371, 982, 1066
Tth111I	GACNNNGTC	1 cut(s)	156
VpaK11BI	GGWCC	4 cut(s)	8, 199, 237, 1243
VspI	ATTAAT	1 cut(s)	45
XapI	RAATTY	4 cut(s)	59, 251, 334, 821
XceI	RCATGY	4 cut(s)	264, 511, 651, 964
XcmI	CCANNNNNNNNNTGG	1 cut(s)	110
XmiI	GTMKAC	1 cut(s)	141
XmnI	GAANNNNTTC	1 cut(s)	360
XspI	CTAG	1 cut(s)	450

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.