Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0003199

Physical Location & Seq

Genomic Coordinates Reverse (-)

20980455 .. 20982491

Gene Structure 2037 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1129300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2037 bp

ATGACTCTCATTCGGAGGATGATTCTCGGCAATGCAAACGGCGTCGTATTCCGACTCATCACTGTGCCTAAAGGTCTCCGTCTTCGTCCACTCGCCAACCCTATATCCAATCCTGCGTCCGTATCCTCTCGTTTTGCCTCAACATCGAAACCCAGAAGTTCCACTCAGACCCGAGACGGCGAAAATTTCTTCGCATTGAACAAAAGGATCTCCCATTTGATCCGAACCGGTCACATAGCCCAAGCCAGGGAAGTGTTTGATCAAATGCCTCTACGAAATGTGGTGACTTGGAATTCAATGATTACTGGGTACGTAAAACGCAGAGAGATGGCGAAGGCGCGCAAGCTGTTCGACGAAATGCCTGAGAGAGACGTTGTAACCTGGAATTTGATGATATCGGGGTATATATCTTGTCGCGGGAGTAGGTACATTGAGGAGGGGAGGATTTTGTTTGATGAAATGCCAGAAAGGGATTGCGTTTCTTGGAACACAATTATTAGTGGGTATGCTAAGAACGGGAGAATGGCGGAGGCGTTAGACCTTTTTAATAGAATGCCGGAGCGGAGTGTTGTTTCTTGGAATGCAATGATTACTGGGTTTTTACAGAATGGTGAAGTTGTGCGCGCAATTGAGTTCTTCGAGAGAATGCCAGAACGAGACGGGGCTTCGCTCAGTGCGCTTGTTTCGGGGCTTATTCAGAATGGCGAACTGGATGAAGCTGCGAGGATAATCATCGAGTGTGGGAATAAGGATGATGGAAGAGAAGATTTGGTGCATGCTTATAATACGTTGATTGCAGGTTATGGTCAGAGAGGACGGGTTGAAGAGGCGCGGAAGCTTTTTGATCAAATCCCGTTTTCACACAAGAAGGGAAAGGAGGGCAATGGGAGATTTGAGAGAAATGTTGTGTCATGGAATTCGATGATTATGTGCTATGTGAAAACTGGAAATGTTGTTTCTGCCAGGGAGCTTTTTGACCAAATGGTAGAACGAGACACCTTTTCGTGGAATACCATGATCAGTGGCTATGTTCATGTGTCAGATATGAAAGAGGCCTCTAGCCTCTTTAGTAAAATGCCAAATCCTGATGCACTTTCATGGAATTCATTGATCTTAGGCTATGCCCAAGTCGGTTCTTTAGAACTGGCTCGTGATTATTTTGAGAAGATGCCCCAAAAGAACCTGGTCACTTGGAACTCCATGATCGCAGGGTATGAGAAAAATGAGGACTTTGTGGGAGCAGTCAATCTCTTTTCTTGGATGCAACTCAAAGGAGAGAAACCTGATAGACACACTTTGTCTTCAGTTCTCAGTGTGAGTACTGGGTTGGTGGATCTAAATCTTGGCATGCAGATTCATCAGCTGGTGGCAAAGACGGTTATTGCAGATATGCCAATAAACAACTCCCTCATAACAATGTATTCGCGATGTGGTGCGATAAGAGAGGCACAGACAATTTTTGATGAAATGAAACTGCAGAAAGATGTGATCTCTTGGAATGCAATGATTGGAGGCTATGCATCTCATGGTTTTGCTGCAGAGGCTTTGGAACTTTTTAGTTTGATGAAGAGGTTAAAGGTACAGCCTACTTATATAACATTTATCGCAGTGTTGAATGCATGTGCTCATGCAGGATTAGTTGAAGAAGGCCGGAGTCAATTTCAATCCATGATTAATGAATTTGGCATTGAGCCAAGTGTTGAACACTATGCCTCCCTTGTGGACAACATCGGTCGGAATGGGCAGCTTGCGGAGGCCATGGATTTGATCAATAGCATGCCATTTGAACCAGACAAGGCTGTATGGGGAGCATTATTAGGTGCTTGTAGAGTGCATAACAATGTAGAGTTGGCCTGTGTTGCAGCTGAAGCATTAATGAGACTCGAACCTGAAAGCTCAGCTCCATATGTACTGTTATACAATATGTATGCTGATGTTGGACAATGGGATGATGCAGCAGACGTGAGATCAATGATGGAAAAGAATAATGTAAGAAAGCATGCAGCCTATAGTCGAGTTGAGTCTTCCAATTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016553 GO:0016554 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR

Protein Analysis

679

Amino Acids

76.22

Weight (kDa)

6.37

Isoelectric Point (pI)

40.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	92 - 123	4.1e-09	PPR repeat family
PPR_1	PF12854	93 - 120	9.1e-06	PPR repeat
PPR	PF01535	95 - 124	2.4e-08	PPR repeat
PPR_1	PF12854	156 - 185	8e-09	PPR repeat
PPR_2	PF13041	158 - 190	5.1e-09	PPR repeat family
PPR	PF01535	160 - 189	8.4e-10	PPR repeat
PPR_2	PF13041	189 - 219	3.6e-06	PPR repeat family
PPR	PF01535	191 - 219	1.5e-06	PPR repeat
PPR	PF01535	260 - 283	4.8e-06	PPR repeat
PPR_1	PF12854	261 - 283	4.3e-06	PPR repeat
PPR_2	PF13041	300 - 331	6.4e-07	PPR repeat family
PPR	PF01535	303 - 331	4e-08	PPR repeat
PPR_2	PF13041	332 - 360	7.3e-06	PPR repeat family
PPR	PF01535	334 - 359	4.7e-06	PPR repeat
PPR_2	PF13041	394 - 437	2.8e-10	PPR repeat family
PPR_2	PF13041	494 - 543	3.6e-10	PPR repeat family
PPR	PF01535	497 - 526	2.4e-07	PPR repeat
E_motif	PF20431	612 - 674	3.6e-13	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003199)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G62260
fragaria_vesca	FvH4_2g07250
malus_domestica	MD10G1129300.v1.1
prunus_persica	Prupe.8G170100_v2.0.a1 Prupe.8G170100_v2.0.a1
pyrus_communis	pycom06g17110 pycom10g11090
rosa_chinensis	RchiOBHm_Chr6g0259851 RchiOBHm_Chr6g0279101
rosa_laevigata	RLG00000013168 RLG00000014504
rosa_multiflora	Rmu_co8522155.1_g000002 Rmu_sc0002080.1_g000004 Rmu_sc0009049.1_g000004 Rmu_sc0037609.1_g000001 Rmu_sc0037609.1_g000002 Rmu_sc0037609.1_g000003
rosa_roxburghii	Rroxscaffold_7G00189130 Rroxscaffold_7G00207040 Rroxscaffold_7G00207050 Rroxscaffold_7G00207060 Rroxscaffold_7G00207180 Rroxscaffold_7G00207190 Rroxscaffold_7G00207200
rosa_rugosa	Rorug05G0590700 Rorug05G0590800 Rorug06G0122200
rosa_samantha	Rh6AG107400 Rh6AG230900 Rh6BG102900 Rh6BG235800 Rh6DG090200 Rh6DG229500
rosa_wichuraiana	Rw0G019930 Rw0G021150 Rw6G009260 Rw6G020220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	783
Acc36I	ACCTGC	1 cut(s)	788
AccBSI	CCGCTC	1 cut(s)	562
AccII	CGCG	5 cut(s)	340, 417, 624, 832, 1426
AciI	CCGC	5 cut(s)	417, 527, 562, 832, 1751
AclWI	GGATC	3 cut(s)	214, 215, 1341
AcsI	RAATTY	6 cut(s)	184, 292, 385, 916, 1102, 1679
AcuI	CTGAAG	2 cut(s)	1287, 1887
AcyI	GRCGYC	1 cut(s)	42
AfaI	GTAC	5 cut(s)	311, 428, 1321, 1581, 1911
AfiI	CCNNNNNNNGG	3 cut(s)	246, 247, 1005
AgeI	ACCGGT	1 cut(s)	227
AgsI	TTSAA	8 cut(s)	199, 297, 824, 1615, 1643, 1664, 1703, 1787
AjiI	CACGTC	1 cut(s)	1963
AjnI	CCWGG	4 cut(s)	245, 380, 962, 1182
AluBI	AGCT	9 cut(s)	346, 719, 838, 970, 1363, 1747, 1865, 1896, 1901
AluI	AGCT	9 cut(s)	346, 719, 838, 970, 1363, 1747, 1865, 1896, 1901
Alw21I	GWGCWC	1 cut(s)	1627
Alw26I	GTCTC	6 cut(s)	80, 168, 363, 651, 987, 1873
AlwI	GGATC	3 cut(s)	214, 215, 1341
Ama87I	CYCGRG	1 cut(s)	171
AoxI	GGCC	4 cut(s)	1053, 1648, 1755, 1851
ApeKI	GCWGC	6 cut(s)	719, 1535, 1744, 1862, 1955, 2003
ApoI	RAATTY	6 cut(s)	184, 292, 385, 916, 1102, 1679
AseI	ATTAAT	2 cut(s)	1674, 1874
AsiGI	ACCGGT	1 cut(s)	227
AspLEI	GCGC	6 cut(s)	340, 342, 624, 626, 679, 832
AsuHPI	GGTGA	2 cut(s)	295, 623
AvaI	CYCGRG	1 cut(s)	171
BauI	CACGAG	1 cut(s)	1149
BbsI	GAAGAC	3 cut(s)	74, 1293, 2016
Bbv12I	GWGCWC	1 cut(s)	1627
BbvI	GCAGC	6 cut(s)	706, 1522, 1756, 1874, 1967, 2015
BccI	CCATC	3 cut(s)	322, 749, 1969
BceAI	ACGGC	2 cut(s)	55, 193
BcgI	CGANNNNNNTGC	2 cut(s)	331, 365
BciT130I	CCWGG	4 cut(s)	247, 382, 964, 1184
BciVI	GTATCC	1 cut(s)	133
BclI	TGATCA	4 cut(s)	259, 844, 1017, 1767
BcoDI	GTCTC	6 cut(s)	80, 168, 363, 651, 987, 1873
BfaI	CTAG	1 cut(s)	1059
BfmI	CTRYAG	3 cut(s)	1475, 1536, 2008
BfuAI	ACCTGC	1 cut(s)	788
BfuI	GTATCC	1 cut(s)	133
BisI	GCNGC	6 cut(s)	720, 1536, 1745, 1863, 1956, 2004
BlpI	GCTNAGC	1 cut(s)	1897
BlsI	GCNGC	6 cut(s)	721, 1537, 1746, 1864, 1957, 2005
BmcAI	AGTACT	1 cut(s)	1321
Bme1390I	CCNGG	4 cut(s)	247, 382, 964, 1184
BmeT110I	CYCGRG	1 cut(s)	171
BmgBI	CACGTC	1 cut(s)	1963
BmrFI	CCNGG	4 cut(s)	247, 382, 964, 1184
BmrI	ACTGGG	3 cut(s)	315, 603, 1332
BmsI	GCATC	5 cut(s)	1078, 1158, 1251, 1529, 1942
BmuI	ACTGGG	3 cut(s)	315, 603, 1332
BpiI	GAAGAC	3 cut(s)	74, 1293, 2016
Bpu1102I	GCTNAGC	1 cut(s)	1897
BsaAI	YACGTR	1 cut(s)	313
BsaBI	GATNNNNATC	2 cut(s)	731, 1338
BsaHI	GRCGYC	1 cut(s)	42
BsaI	GGTCTC	1 cut(s)	80
BsaJI	CCNNGG	3 cut(s)	246, 963, 1758
BsaWI	WCCGGW	1 cut(s)	227
BsaXI	ACNNNNNCTCC	2 cut(s)	1697, 1727
Bsc4I	CCNNNNNNNGG	3 cut(s)	246, 247, 1005
Bse118I	RCCGGY	1 cut(s)	227
Bse1I	ACTGG	6 cut(s)	310, 598, 714, 949, 1149, 1327
Bse3DI	GCAATG	4 cut(s)	37, 591, 889, 1509
Bse8I	GATNNNNATC	2 cut(s)	731, 1338
BseBI	CCWGG	4 cut(s)	247, 382, 964, 1184
BseDI	CCNNGG	3 cut(s)	246, 963, 1758
BseGI	GGATG	5 cut(s)	24, 718, 757, 1266, 1954
BseJI	GATNNNNATC	2 cut(s)	731, 1338
BseLI	CCNNNNNNNGG	3 cut(s)	246, 247, 1005
BseMI	GCAATG	4 cut(s)	37, 591, 889, 1509
BseMII	CTCAG	5 cut(s)	179, 354, 685, 1324, 1911
BseNI	ACTGG	6 cut(s)	310, 598, 714, 949, 1149, 1327
BsePI	GCGCGC	2 cut(s)	338, 622
BseRI	GAGGAG	1 cut(s)	449
BseXI	GCAGC	6 cut(s)	706, 1522, 1756, 1874, 1967, 2015
Bsh1236I	CGCG	5 cut(s)	340, 417, 624, 832, 1426
Bsh1285I	CGRYCG	1 cut(s)	1735
BshFI	GGCC	4 cut(s)	1055, 1650, 1757, 1853
BshTI	ACCGGT	1 cut(s)	227
BsiEI	CGRYCG	1 cut(s)	1735
BsiHKAI	GWGCWC	1 cut(s)	1627
BsiHKCI	CYCGRG	1 cut(s)	171
BsiSI	CCGG	3 cut(s)	228, 557, 1651
BslI	CCNNNNNNNGG	3 cut(s)	246, 247, 1005
BsmAI	GTCTC	6 cut(s)	80, 168, 363, 651, 987, 1873
BsmBI	CGTCTC	3 cut(s)	168, 363, 651
BsmI	GAATGC	5 cut(s)	558, 586, 651, 1504, 1621
BsnI	GGCC	4 cut(s)	1055, 1650, 1757, 1853
Bso31I	GGTCTC	1 cut(s)	80
BsoBI	CYCGRG	1 cut(s)	171
Bsp1286I	GDGCHC	1 cut(s)	1627
Bsp1720I	GCTNAGC	1 cut(s)	1897
Bsp19I	CCATGG	1 cut(s)	1758
Bsp68I	TCGCGA	1 cut(s)	1426
BspACI	CCGC	5 cut(s)	417, 527, 562, 832, 1751
BspANI	GGCC	4 cut(s)	1055, 1650, 1757, 1853
BspCNI	CTCAG	5 cut(s)	178, 355, 684, 1323, 1910
BspFNI	CGCG	5 cut(s)	340, 417, 624, 832, 1426
BspMAI	CTGCAG	2 cut(s)	1479, 1540
BspMI	ACCTGC	1 cut(s)	788
BspPI	GGATC	3 cut(s)	214, 215, 1341
BspTNI	GGTCTC	1 cut(s)	80
BsrBI	CCGCTC	1 cut(s)	562
BsrDI	GCAATG	4 cut(s)	37, 591, 889, 1509
BsrFI	RCCGGY	1 cut(s)	227
BsrI	ACTGG	6 cut(s)	310, 598, 714, 949, 1149, 1327
BssAI	RCCGGY	1 cut(s)	227
BssECI	CCNNGG	3 cut(s)	246, 963, 1758
BssHII	GCGCGC	2 cut(s)	338, 622
BssNI	GRCGYC	1 cut(s)	42
BssSI	CACGAG	1 cut(s)	1149
BssT1I	CCWWGG	1 cut(s)	1758
Bst2BI	CACGAG	1 cut(s)	1149
Bst2UI	CCWGG	4 cut(s)	247, 382, 964, 1184
Bst4CI	ACNGT	3 cut(s)	64, 1378, 1914
Bst6I	CTCTTC	3 cut(s)	754, 819, 1562
BstACI	GRCGYC	1 cut(s)	42
BstBAI	YACGTR	1 cut(s)	313
BstC8I	GCNNGC	8 cut(s)	340, 344, 624, 777, 1349, 1749, 1778, 2001
BstDEI	CTNAG	7 cut(s)	165, 363, 510, 671, 1114, 1310, 1897
BstDSI	CCRYGG	1 cut(s)	1758
BstF5I	GGATG	5 cut(s)	24, 718, 757, 1266, 1954
BstFNI	CGCG	5 cut(s)	340, 417, 624, 832, 1426
BstHHI	GCGC	6 cut(s)	340, 342, 624, 626, 679, 832
BstMAI	GTCTC	6 cut(s)	80, 168, 363, 651, 987, 1873
BstMCI	CGRYCG	1 cut(s)	1735
BstMWI	GCNNNNNNNGC	4 cut(s)	676, 1541, 1859, 1868
BstNI	CCWGG	4 cut(s)	247, 382, 964, 1184
BstNSI	RCATGY	5 cut(s)	779, 1351, 1623, 1780, 2003
BstSCI	CCNGG	4 cut(s)	245, 380, 962, 1182
BstSFI	CTRYAG	3 cut(s)	1475, 1536, 2008
BstSNI	TACGTA	1 cut(s)	313
BstUI	CGCG	5 cut(s)	340, 417, 624, 832, 1426
BstV1I	GCAGC	6 cut(s)	706, 1522, 1756, 1874, 1967, 2015
BstV2I	GAAGAC	3 cut(s)	74, 1293, 2016
BstX2I	RGATCY	2 cut(s)	207, 1333
BstYI	RGATCY	2 cut(s)	207, 1333
BsuI	GTATCC	1 cut(s)	133
BsuRI	GGCC	4 cut(s)	1055, 1650, 1757, 1853
BtgI	CCRYGG	1 cut(s)	1758
BtgZI	GCGATG	1 cut(s)	1441
BtrI	CACGTC	1 cut(s)	1963
BtsCI	GGATG	5 cut(s)	24, 718, 757, 1266, 1954
BtsI	GCAGTG	1 cut(s)	1614
BtsIMutI	CAGTG	5 cut(s)	60, 679, 1027, 1318, 1614
BtuMI	TCGCGA	1 cut(s)	1426
BveI	ACCTGC	1 cut(s)	788
Cac8I	GCNNGC	8 cut(s)	340, 344, 624, 777, 1349, 1749, 1778, 2001
CfoI	GCGC	6 cut(s)	340, 342, 624, 626, 679, 832
Cfr10I	RCCGGY	1 cut(s)	227
CseI	GACGC	2 cut(s)	31, 105
CsiI	ACCWGGT	1 cut(s)	1182
Csp6I	GTAC	5 cut(s)	310, 427, 1320, 1580, 1910
CspAI	ACCGGT	1 cut(s)	227
CviQI	GTAC	5 cut(s)	310, 427, 1320, 1580, 1910
DdeI	CTNAG	7 cut(s)	165, 363, 510, 671, 1114, 1310, 1897
Eam1104I	CTCTTC	3 cut(s)	754, 819, 1562
EarI	CTCTTC	3 cut(s)	754, 819, 1562
EciI	GGCGGA	1 cut(s)	542
Eco105I	TACGTA	1 cut(s)	313
Eco130I	CCWWGG	1 cut(s)	1758
Eco147I	AGGCCT	1 cut(s)	1055
Eco31I	GGTCTC	1 cut(s)	80
Eco32I	GATATC	1 cut(s)	396
Eco57I	CTGAAG	2 cut(s)	1287, 1887
Eco88I	CYCGRG	1 cut(s)	171
EcoRI	GAATTC	3 cut(s)	292, 916, 1102
EcoRII	CCWGG	4 cut(s)	245, 380, 962, 1182
EcoRV	GATATC	1 cut(s)	396
EcoT14I	CCWWGG	1 cut(s)	1758
EcoT22I	ATGCAT	2 cut(s)	1522, 1621
ErhI	CCWWGG	1 cut(s)	1758
Esp3I	CGTCTC	3 cut(s)	168, 363, 651
FauI	CCCGC	1 cut(s)	410
FauNDI	CATATG	1 cut(s)	1906
FbaI	TGATCA	4 cut(s)	259, 844, 1017, 1767
Fnu4HI	GCNGC	6 cut(s)	720, 1536, 1745, 1863, 1956, 2004
FokI	GGATG	5 cut(s)	31, 725, 764, 1273, 1961
Fsp4HI	GCNGC	6 cut(s)	720, 1536, 1745, 1863, 1956, 2004
FspBI	CTAG	1 cut(s)	1059
GlaI	GCGC	6 cut(s)	339, 341, 623, 625, 678, 831
GluI	GCNGC	6 cut(s)	720, 1536, 1745, 1863, 1956, 2004
HaeIII	GGCC	4 cut(s)	1055, 1650, 1757, 1853
HapII	CCGG	3 cut(s)	228, 557, 1651
HgaI	GACGC	2 cut(s)	31, 105
HhaI	GCGC	6 cut(s)	340, 342, 624, 626, 679, 832
Hin1I	GRCGYC	1 cut(s)	42
Hin6I	GCGC	6 cut(s)	338, 340, 622, 624, 677, 830
HinP1I	GCGC	6 cut(s)	338, 340, 622, 624, 677, 830
HindIII	AAGCTT	1 cut(s)	836
HinfI	GANTC	7 cut(s)	4, 22, 54, 1354, 1654, 1881, 2021
HpaII	CCGG	3 cut(s)	228, 557, 1651
HphI	GGTGA	2 cut(s)	295, 623
Hpy166II	GTNNAC	2 cut(s)	89, 1723
Hpy188I	TCNGA	8 cut(s)	15, 53, 168, 224, 699, 810, 1042, 1737
Hpy188III	TCNNGA	4 cut(s)	640, 1085, 1151, 1425
Hpy8I	GTNNAC	2 cut(s)	89, 1723
Hpy99I	CGWCG	2 cut(s)	47, 356
HpyAV	CCTTC	3 cut(s)	328, 862, 1640
HpyCH4III	ACNGT	3 cut(s)	64, 1378, 1914
HpyCH4IV	ACGT	4 cut(s)	312, 372, 788, 1962
HpyF10VI	GCNNNNNNNGC	4 cut(s)	676, 1541, 1859, 1868
HpyF3I	CTNAG	7 cut(s)	165, 363, 510, 671, 1114, 1310, 1897
HpySE526I	ACGT	4 cut(s)	312, 372, 788, 1962
Hsp92I	GRCGYC	1 cut(s)	42
HspAI	GCGC	6 cut(s)	338, 340, 622, 624, 677, 830
Ksp22I	TGATCA	4 cut(s)	259, 844, 1017, 1767
LmnI	GCTCC	5 cut(s)	559, 967, 1238, 1808, 1906
Lsp1109I	GCAGC	6 cut(s)	706, 1522, 1756, 1874, 1967, 2015
LweI	GCATC	5 cut(s)	1078, 1158, 1251, 1529, 1942
MabI	ACCWGGT	1 cut(s)	1182
MaeI	CTAG	1 cut(s)	1059
MaeII	ACGT	4 cut(s)	312, 372, 788, 1962
MaeIII	GTNAC	4 cut(s)	230, 283, 376, 1186
MbiI	CCGCTC	1 cut(s)	562
MfeI	CAATTG	1 cut(s)	627
MflI	RGATCY	2 cut(s)	207, 1333
MhlI	GDGCHC	1 cut(s)	1627
MlyI	GAGTC	4 cut(s)	48, 1663, 1875, 2030
MmeI	TCCRAC	3 cut(s)	76, 1715, 1918
Mph1103I	ATGCAT	2 cut(s)	1522, 1621
MseI	TTAA	4 cut(s)	546, 1574, 1674, 1874
MslI	CAYNNNNRTG	4 cut(s)	62, 1096, 1415, 1839
MspA1I	CMGCKG	2 cut(s)	1363, 1865
MspI	CCGG	3 cut(s)	228, 557, 1651
MspR9I	CCNGG	4 cut(s)	247, 382, 964, 1184
MunI	CAATTG	1 cut(s)	627
Mva1269I	GAATGC	5 cut(s)	558, 586, 651, 1504, 1621
MvaI	CCWGG	4 cut(s)	247, 382, 964, 1184
MvnI	CGCG	5 cut(s)	340, 417, 624, 832, 1426
MwoI	GCNNNNNNNGC	4 cut(s)	676, 1541, 1859, 1868
NcoI	CCATGG	1 cut(s)	1758
NdeI	CATATG	1 cut(s)	1906
NmeAIII	GCCGAG	1 cut(s)	6
NmuCI	GTSAC	3 cut(s)	230, 283, 1186
NruI	TCGCGA	1 cut(s)	1426
NsiI	ATGCAT	2 cut(s)	1522, 1621
NspI	RCATGY	5 cut(s)	779, 1351, 1623, 1780, 2003
PaeI	GCATGC	4 cut(s)	779, 1351, 1780, 2003
PauI	GCGCGC	2 cut(s)	338, 622
PceI	AGGCCT	1 cut(s)	1055
PctI	GAATGC	5 cut(s)	558, 586, 651, 1504, 1621
PfeI	GAWTC	2 cut(s)	22, 1354
PinAI	ACCGGT	1 cut(s)	227
PkrI	GCNGC	6 cut(s)	721, 1537, 1746, 1864, 1957, 2005
PleI	GAGTC	4 cut(s)	48, 1662, 1875, 2029
PpsI	GAGTC	4 cut(s)	48, 1662, 1875, 2029
Ppu21I	YACGTR	1 cut(s)	313
PshBI	ATTAAT	2 cut(s)	1674, 1874
PsiI	TTATAA	1 cut(s)	783
Psp6I	CCWGG	4 cut(s)	245, 380, 962, 1182
PspGI	CCWGG	4 cut(s)	245, 380, 962, 1182
PstI	CTGCAG	2 cut(s)	1479, 1540
PsuI	RGATCY	2 cut(s)	207, 1333
PteI	GCGCGC	2 cut(s)	338, 622
PvuII	CAGCTG	2 cut(s)	1363, 1865
RruI	TCGCGA	1 cut(s)	1426
RsaI	GTAC	5 cut(s)	311, 428, 1321, 1581, 1911
RsaNI	GTAC	5 cut(s)	310, 427, 1320, 1580, 1910
RseI	CAYNNNNRTG	4 cut(s)	62, 1096, 1415, 1839
SaqAI	TTAA	4 cut(s)	546, 1574, 1674, 1874
SatI	GCNGC	6 cut(s)	720, 1536, 1745, 1863, 1956, 2004
ScaI	AGTACT	1 cut(s)	1321
SchI	GAGTC	4 cut(s)	48, 1663, 1875, 2030
ScrFI	CCNGG	4 cut(s)	247, 382, 964, 1184
SduI	GDGCHC	1 cut(s)	1627
SexAI	ACCWGGT	1 cut(s)	1182
SfaNI	GCATC	5 cut(s)	1078, 1158, 1251, 1529, 1942
SfcI	CTRYAG	3 cut(s)	1475, 1536, 2008
SmiMI	CAYNNNNRTG	4 cut(s)	62, 1096, 1415, 1839
SnaBI	TACGTA	1 cut(s)	313
SphI	GCATGC	4 cut(s)	779, 1351, 1780, 2003
SseBI	AGGCCT	1 cut(s)	1055
SsiI	CCGC	5 cut(s)	417, 527, 562, 832, 1751
SspMI	CTAG	1 cut(s)	1059
StuI	AGGCCT	1 cut(s)	1055
StyD4I	CCNGG	4 cut(s)	245, 380, 962, 1182
StyI	CCWWGG	1 cut(s)	1758
TaaI	ACNGT	3 cut(s)	64, 1378, 1914
TaiI	ACGT	4 cut(s)	315, 375, 791, 1965
TaqI	TCGA	7 cut(s)	146, 351, 639, 735, 920, 1884, 2014
TaqII	GACCGA	1 cut(s)	1721
TatI	WGTACW	2 cut(s)	1319, 1909
TfiI	GAWTC	2 cut(s)	22, 1354
Tru1I	TTAA	4 cut(s)	546, 1574, 1674, 1874
Tru9I	TTAA	4 cut(s)	546, 1574, 1674, 1874
TscAI	CASTG	5 cut(s)	67, 679, 1027, 1318, 1614
TseFI	GTSAC	3 cut(s)	230, 283, 1186
TseI	GCWGC	6 cut(s)	719, 1535, 1744, 1862, 1955, 2003
Tsp45I	GTSAC	3 cut(s)	230, 283, 1186
TspGWI	ACGGA	2 cut(s)	68, 109
TspRI	CASTG	5 cut(s)	67, 679, 1027, 1318, 1614
VspI	ATTAAT	2 cut(s)	1674, 1874
XapI	RAATTY	6 cut(s)	184, 292, 385, 916, 1102, 1679
XceI	RCATGY	5 cut(s)	779, 1351, 1623, 1780, 2003
XspI	CTAG	1 cut(s)	1059
ZrmI	AGTACT	1 cut(s)	1321
Zsp2I	ATGCAT	2 cut(s)	1522, 1621

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.