Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Contig00897

Orthogroup ID

OG0003199

Physical Location & Seq

Genomic Coordinates Reverse (-)

18841 .. 21660

Gene Structure 2820 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw0G019930.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2022 bp

ATGATGATTCTGGGTAATGCAAATGGTTTCATATATAAACTCATGCCCCGAGCTCGTATTATTCTTCGTCTACTCACCATCCCATTTCCCGATAATCACGCATCCCCACAGCACTCTTGTTTTGCCTCAACTTCAAAACCCGGCAGTCATGCGAACGACGTAGACCTTTTTTCACTGAACAGAAGGATCTCCCATTTGATCCGAACTGGTCAGATAGAGCAAGCCAGGGAAGCCTTTGACAAGATGAAGCAACGGAATATCGTTACTTGGAATTCAATGATAACCGGGTATGTGAAACGCCGAGAGATGGCCAAGGCCCGGAAGCTGTTCGACGAAATGCCTGACAGAGATGTTGTGTCGTGGAATTTGATGATATCCGGGTACGTTTCGTGCCGCGGAAGTCGTCGGTATGTTGAGGAGGGGAGGACTTTGTTTGATCAGATGCCGGTGAGGGACTGTGTTTCTTGGAACACAATGATCAGTGGGTACGCCAAGAATGGGAGGATGGGGGAGGCATTGCGGCTTTTCGATGCCATGCCGCAGAGGAGTGTTGTTTCTTGGAATGCAATGGTTACCGGGTTTCTACAGAATGGTGATGTTGCGCGTGCGATTGAGTTTTTCGAGAGAATGCCGCAGAGGGATGGGGCTTCTTTTAGTGCACTCGTGTCGGGGCTTATTCAGAATGGTGAGCTGGATGAAGCTGCGAGGATTGTTGGTGAATGTGGGAAGAGGGATGAGGAGGGGGTGGAAGATTTGGTGGATGCTTATAATACGTTGGTTGCTGGTTATGGTCAGAGAGGAAGGGTTGAAGAGGCTCGGCGCCTTTTTGATCAAATACCATTTCGTCGTGAGAAGGGGAATGAGGACAGTGGGAGGTTTGAGAGGAATGTTGTATCGTGGAATTCTATGATTATGTGCTATATGAAAACAGGAAATATTGTTTCTGCTAGAGAACTTTTTGACCAAATGATAGAACGAGATACCTTTTCGTGGAATACCATGATCAGCGGCTATGTTCATATGTCAGATATGGAAGAGGCTGCCAATCTCTTTCGTAGAATGCCAAATCCTGATACACTGTCATGGAATGCAATGATCTTAGGGTATGCCCAGGTTGGGCGTTTAAAACTGGCTCATGATTTTTTTGAGAGGATGCCGCAGAAGAGTCTAATATCTTGGAACTCCATGATAGCAGGGTATGAGAAAAATGAGGACTTTATGGGTGGAGTTAAGCTTTTCTCTCAGATGCAACTAGAAGGAGAGAAACCTGATAGACACACTTTATCATCAGTTCTTAGTGTGAGTACTGGGTTAGCGGATCTGCATCTAGGCATGCAGATTCATCAGCTGGTCATGAAGACAGTTATTGCAGATTTGCCAATAAACAACTCTCTTATAACTATGTATTCACGATGTGGTGCTATCAAAGAGGCAGAAACAATTTTTGTTGAGATGAAACAGCAGAAAGATGTGATCTCCTGGAATGCAATGATTGGGGGCTATGCATCTCATGGTTTTGCTCAAGAGGCTTTAGGACTTTTTTGTTTGATGAAGAGGTCAAAGGTGCAACCTACATATATAACATTTATTGCAGTTTTAAATGCATGTGCTCATGCAGGATTAGTTGAAGAAGGCCGGAGTCAATTTAAATCCATGATTAGTGAATTTGGCATTGAGCCACGTATCGAACACTATGCCTCCCTTGTCGACATCATTGGTCGGCATGGGCAGCTTGAGGAGGCTCACGATTTGATCAATAGTATGCCATTTGAACCTGACAAGGCTGTGTGGGGAGCATTATTAGGTGCTTGTAGAGTGCATAACAATGTAGAGTTGGCAAAAGTTGCAGCTGAAGCATTAACAAGACTTGAACCTGAAAGCTCAGGTCCGTATGTATTGTTGTACAATATGTATGCTGATGCCGGACTATGGGATGAAGCAGCAGCTGTGAGATCGCTGATGGATGAAAATAAGATAACAAAGCATGCTGCCTATAGTAGGGTAAATTCTTCGCATTGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016553 GO:0016554 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR

Protein Analysis

673

Amino Acids

76.0

Weight (kDa)

6.41

Isoelectric Point (pI)

45.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	85 - 117	4.9e-09	PPR repeat family
PPR	PF01535	88 - 117	5.4e-08	PPR repeat
PPR_1	PF12854	151 - 179	1.6e-08	PPR repeat
PPR_2	PF13041	152 - 184	1.5e-08	PPR repeat family
PPR	PF01535	154 - 182	1.2e-09	PPR repeat
PPR_2	PF13041	295 - 326	1.3e-06	PPR repeat family
PPR	PF01535	298 - 326	1.4e-07	PPR repeat
PPR	PF01535	329 - 354	1.5e-06	PPR repeat
PPR_2	PF13041	390 - 432	1.5e-07	PPR repeat family
PPR_2	PF13041	489 - 538	3e-10	PPR repeat family
PPR	PF01535	492 - 521	1.1e-07	PPR repeat
E_motif	PF20431	607 - 666	5.6e-13	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0003199)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G62260
fragaria_vesca	FvH4_2g07250
malus_domestica	MD10G1129300.v1.1
prunus_persica	Prupe.8G170100_v2.0.a1 Prupe.8G170100_v2.0.a1
pyrus_communis	pycom06g17110 pycom10g11090
rosa_chinensis	RchiOBHm_Chr6g0259851 RchiOBHm_Chr6g0279101
rosa_laevigata	RLG00000013168 RLG00000014504
rosa_multiflora	Rmu_co8522155.1_g000002 Rmu_sc0002080.1_g000004 Rmu_sc0009049.1_g000004 Rmu_sc0037609.1_g000001 Rmu_sc0037609.1_g000002 Rmu_sc0037609.1_g000003
rosa_roxburghii	Rroxscaffold_7G00189130 Rroxscaffold_7G00207040 Rroxscaffold_7G00207050 Rroxscaffold_7G00207060 Rroxscaffold_7G00207180 Rroxscaffold_7G00207190 Rroxscaffold_7G00207200
rosa_rugosa	Rorug05G0590700 Rorug05G0590800 Rorug06G0122200
rosa_samantha	Rh6AG107400 Rh6AG230900 Rh6BG102900 Rh6BG235800 Rh6DG090200 Rh6DG229500
rosa_wichuraiana	Rw0G019930 Rw0G021150 Rw6G009260 Rw6G020220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	768, 1397
AccB1I	GGYRCC	1 cut(s)	819
AccI	GTMKAC	3 cut(s)	70, 162, 1707
AccII	CGCG	2 cut(s)	396, 604
AciI	CCGC	8 cut(s)	394, 396, 520, 539, 632, 1008, 1157, 1316
AclWI	GGATC	3 cut(s)	193, 194, 1326
AcoI	YGGCCR	1 cut(s)	309
AcsI	RAATTY	5 cut(s)	271, 364, 901, 1664, 2005
AcuI	CTGAAG	1 cut(s)	1872
AcyI	GRCGYC	1 cut(s)	820
AdeI	CACNNNGTG	1 cut(s)	1415
AfaI	GTAC	4 cut(s)	383, 488, 1306, 1904
AfiI	CCNNNNNNNGG	7 cut(s)	307, 318, 498, 990, 1116, 1929, 1998
AgsI	TTSAA	6 cut(s)	135, 276, 809, 1628, 1772, 1871
AjnI	CCWGG	3 cut(s)	224, 1110, 1478
Alw21I	GWGCWC	3 cut(s)	55, 661, 1612
Alw44I	GTGCAC	1 cut(s)	657
AlwI	GGATC	3 cut(s)	193, 194, 1326
AlwNI	CAGNNNCTG	1 cut(s)	1946
Ama87I	CYCGRG	1 cut(s)	48
AoxI	GGCC	3 cut(s)	309, 315, 1633
ApaLI	GTGCAC	1 cut(s)	657
ApeKI	GCWGC	7 cut(s)	701, 1040, 1729, 1847, 1940, 1943, 1988
ApoI	RAATTY	5 cut(s)	271, 364, 901, 1664, 2005
Asp700I	GAANNNNTTC	1 cut(s)	326
AspLEI	GCGC	2 cut(s)	604, 822
AspS9I	GGNCC	2 cut(s)	316, 1886
AsuC2I	CCSGG	5 cut(s)	141, 286, 319, 379, 577
AsuHPI	GGTGA	5 cut(s)	67, 460, 605, 698, 728
AvaI	CYCGRG	1 cut(s)	48
AvaII	GGWCC	1 cut(s)	1886
BaeGI	GKGCMC	1 cut(s)	661
BalI	TGGCCA	1 cut(s)	311
BanI	GGYRCC	1 cut(s)	819
BanII	GRGCYC	1 cut(s)	55
BauI	CACGAG	1 cut(s)	662
BbsI	GAAGAC	1 cut(s)	1364
Bbv12I	GWGCWC	3 cut(s)	55, 661, 1612
BbvI	GCAGC	7 cut(s)	688, 1027, 1741, 1859, 1952, 1955, 1975
BccI	CCATC	5 cut(s)	86, 301, 499, 635, 1954
BcgI	CGANNNNNNTGC	4 cut(s)	648, 682, 1676, 1710
BciT130I	CCWGG	3 cut(s)	226, 1112, 1480
BclI	TGATCA	5 cut(s)	436, 477, 829, 1002, 1752
BcnI	CCSGG	5 cut(s)	141, 286, 319, 379, 577
BfaI	CTAG	3 cut(s)	948, 1253, 1328
BfmI	CTRYAG	2 cut(s)	584, 1993
BfoI	RGCGCY	1 cut(s)	823
BmcAI	AGTACT	1 cut(s)	1306
Bme1390I	CCNGG	8 cut(s)	141, 226, 286, 319, 379, 577, 1112, 1480
Bme18I	GGWCC	1 cut(s)	1886
BmeT110I	CYCGRG	1 cut(s)	48
BmgT120I	GGNCC	2 cut(s)	316, 1886
BmiI	GGNNCC	1 cut(s)	821
BmrFI	CCNGG	8 cut(s)	141, 226, 286, 319, 379, 577, 1112, 1480
BmrI	ACTGGG	1 cut(s)	1317
BmsI	GCATC	9 cut(s)	110, 432, 520, 751, 1143, 1236, 1333, 1514, 1909
BmuI	ACTGGG	1 cut(s)	1317
BpiI	GAAGAC	1 cut(s)	1364
Bpu10I	CCTNAGC	1 cut(s)	1882
BpuEI	CTTGAG	2 cut(s)	1506, 1754
BpuMI	CCSGG	5 cut(s)	141, 286, 319, 379, 577
BsaAI	YACGTR	1 cut(s)	1682
BsaBI	GATNNNNATC	1 cut(s)	1323
BsaHI	GRCGYC	1 cut(s)	820
BsaJI	CCNNGG	4 cut(s)	225, 312, 394, 1110
BsaXI	ACNNNNNCTCC	2 cut(s)	1682, 1712
Bsc4I	CCNNNNNNNGG	7 cut(s)	307, 318, 498, 990, 1116, 1929, 1998
Bse118I	RCCGGY	1 cut(s)	445
Bse1I	ACTGG	3 cut(s)	211, 1134, 1312
Bse3DI	GCAATG	5 cut(s)	515, 573, 1098, 1494, 2014
Bse8I	GATNNNNATC	1 cut(s)	1323
BseBI	CCWGG	3 cut(s)	226, 1112, 1480
BseDI	CCNNGG	4 cut(s)	225, 312, 394, 1110
BseJI	GATNNNNATC	1 cut(s)	1323
BseLI	CCNNNNNNNGG	7 cut(s)	307, 318, 498, 990, 1116, 1929, 1998
BseMI	GCAATG	5 cut(s)	515, 573, 1098, 1494, 2014
BseMII	CTCAG	2 cut(s)	1256, 1896
BseNI	ACTGG	3 cut(s)	211, 1134, 1312
BseRI	GAGGAG	4 cut(s)	431, 559, 752, 1751
BseSI	GKGCMC	1 cut(s)	661
BseXI	GCAGC	7 cut(s)	688, 1027, 1741, 1859, 1952, 1955, 1975
Bsh1236I	CGCG	2 cut(s)	396, 604
BshFI	GGCC	3 cut(s)	311, 317, 1635
BshNI	GGYRCC	1 cut(s)	819
BsiHKAI	GWGCWC	3 cut(s)	55, 661, 1612
BsiHKCI	CYCGRG	1 cut(s)	48
BsiSI	CCGG	8 cut(s)	141, 285, 319, 378, 446, 576, 1636, 1923
BslFI	GGGAC	1 cut(s)	467
BslI	CCNNNNNNNGG	7 cut(s)	307, 318, 498, 990, 1116, 1929, 1998
BsmFI	GGGAC	1 cut(s)	467
BsmI	GAATGC	5 cut(s)	568, 633, 1065, 1093, 1489
BsnI	GGCC	3 cut(s)	311, 317, 1635
BsoBI	CYCGRG	1 cut(s)	48
Bsp1286I	GDGCHC	3 cut(s)	55, 661, 1612
Bsp1407I	TGTACA	1 cut(s)	1902
BspACI	CCGC	8 cut(s)	394, 396, 520, 539, 632, 1008, 1157, 1316
BspANI	GGCC	3 cut(s)	311, 317, 1635
BspCNI	CTCAG	2 cut(s)	1255, 1895
BspFNI	CGCG	2 cut(s)	396, 604
BspHI	TCATGA	2 cut(s)	1135, 1353
BspLI	GGNNCC	1 cut(s)	821
BspPI	GGATC	3 cut(s)	193, 194, 1326
BspT107I	GGYRCC	1 cut(s)	819
BsrDI	GCAATG	5 cut(s)	515, 573, 1098, 1494, 2014
BsrFI	RCCGGY	1 cut(s)	445
BsrGI	TGTACA	1 cut(s)	1902
BsrI	ACTGG	3 cut(s)	211, 1134, 1312
BssAI	RCCGGY	1 cut(s)	445
BssECI	CCNNGG	4 cut(s)	225, 312, 394, 1110
BssNI	GRCGYC	1 cut(s)	820
BssSI	CACGAG	1 cut(s)	662
BssT1I	CCWWGG	1 cut(s)	312
Bst2BI	CACGAG	1 cut(s)	662
Bst2UI	CCWGG	3 cut(s)	226, 1112, 1480
Bst4CI	ACNGT	4 cut(s)	458, 869, 1080, 1363
Bst6I	CTCTTC	5 cut(s)	722, 804, 1029, 1157, 1547
BstACI	GRCGYC	1 cut(s)	820
BstAPI	GCANNNNNTGC	1 cut(s)	1844
BstAUI	TGTACA	1 cut(s)	1902
BstBAI	YACGTR	1 cut(s)	1682
BstC8I	GCNNGC	4 cut(s)	222, 606, 1334, 1986
BstDEI	CTNAG	4 cut(s)	1099, 1242, 1295, 1882
BstDSI	CCRYGG	1 cut(s)	394
BstEII	GGTNACC	1 cut(s)	571
BstENI	CCTNNNNNAGG	1 cut(s)	1996
BstFNI	CGCG	2 cut(s)	396, 604
BstH2I	RGCGCY	1 cut(s)	823
BstHHI	GCGC	2 cut(s)	604, 822
BstMWI	GCNNNNNNNGC	5 cut(s)	230, 1526, 1729, 1844, 1853
BstNI	CCWGG	3 cut(s)	226, 1112, 1480
BstNSI	RCATGY	3 cut(s)	1336, 1608, 1988
BstPI	GGTNACC	1 cut(s)	571
BstSCI	CCNGG	8 cut(s)	139, 224, 284, 317, 377, 575, 1110, 1478
BstSFI	CTRYAG	2 cut(s)	584, 1993
BstSLI	GKGCMC	1 cut(s)	661
BstUI	CGCG	2 cut(s)	396, 604
BstV1I	GCAGC	7 cut(s)	688, 1027, 1741, 1859, 1952, 1955, 1975
BstV2I	GAAGAC	1 cut(s)	1364
BstX2I	RGATCY	2 cut(s)	186, 1318
BstYI	RGATCY	2 cut(s)	186, 1318
BsuRI	GGCC	3 cut(s)	311, 317, 1635
BtgI	CCRYGG	1 cut(s)	394
BtsIMutI	CAGTG	4 cut(s)	173, 487, 874, 1076
Cac8I	GCNNGC	4 cut(s)	222, 606, 1334, 1986
CaiI	CAGNNNCTG	1 cut(s)	1946
CciI	TCATGA	2 cut(s)	1135, 1353
CfoI	GCGC	2 cut(s)	604, 822
Cfr10I	RCCGGY	1 cut(s)	445
Cfr13I	GGNCC	2 cut(s)	316, 1886
Cfr42I	CCGCGG	1 cut(s)	397
Csp6I	GTAC	4 cut(s)	382, 487, 1305, 1903
CviQI	GTAC	4 cut(s)	382, 487, 1305, 1903
DdeI	CTNAG	4 cut(s)	1099, 1242, 1295, 1882
DinI	GGCGCC	1 cut(s)	821
DraI	TTTAAA	3 cut(s)	1125, 1599, 1648
DraIII	CACNNNGTG	1 cut(s)	1415
EaeI	YGGCCR	1 cut(s)	309
Eam1104I	CTCTTC	5 cut(s)	722, 804, 1029, 1157, 1547
EarI	CTCTTC	5 cut(s)	722, 804, 1029, 1157, 1547
Ecl136II	GAGCTC	1 cut(s)	53
Eco130I	CCWWGG	1 cut(s)	312
Eco24I	GRGCYC	1 cut(s)	55
Eco32I	GATATC	1 cut(s)	375
Eco47I	GGWCC	1 cut(s)	1886
Eco53kI	GAGCTC	1 cut(s)	53
Eco57I	CTGAAG	1 cut(s)	1872
Eco88I	CYCGRG	1 cut(s)	48
Eco91I	GGTNACC	1 cut(s)	571
EcoICRI	GAGCTC	1 cut(s)	53
EcoNI	CCTNNNNNAGG	1 cut(s)	1996
EcoO65I	GGTNACC	1 cut(s)	571
EcoRI	GAATTC	2 cut(s)	271, 901
EcoRII	CCWGG	3 cut(s)	224, 1110, 1478
EcoRV	GATATC	1 cut(s)	375
EcoT14I	CCWWGG	1 cut(s)	312
EcoT22I	ATGCAT	2 cut(s)	1507, 1606
EcoT38I	GRGCYC	1 cut(s)	55
EgeI	GGCGCC	1 cut(s)	821
EheI	GGCGCC	1 cut(s)	821
ErhI	CCWWGG	1 cut(s)	312
FaqI	GGGAC	1 cut(s)	467
FauNDI	CATATG	1 cut(s)	1020
FbaI	TGATCA	5 cut(s)	436, 477, 829, 1002, 1752
FblI	GTMKAC	3 cut(s)	70, 162, 1707
FriOI	GRGCYC	1 cut(s)	55
FspBI	CTAG	3 cut(s)	948, 1253, 1328
GlaI	GCGC	2 cut(s)	603, 821
HaeII	RGCGCY	1 cut(s)	823
HaeIII	GGCC	3 cut(s)	311, 317, 1635
HapII	CCGG	8 cut(s)	141, 285, 319, 378, 446, 576, 1636, 1923
HhaI	GCGC	2 cut(s)	604, 822
Hin1I	GRCGYC	1 cut(s)	820
Hin6I	GCGC	2 cut(s)	602, 820
HinP1I	GCGC	2 cut(s)	602, 820
HincII	GTYRAC	1 cut(s)	1708
HindII	GTYRAC	1 cut(s)	1708
HindIII	AAGCTT	1 cut(s)	1232
HinfI	GANTC	4 cut(s)	7, 1165, 1339, 1639
HpaII	CCGG	8 cut(s)	141, 285, 319, 378, 446, 576, 1636, 1923
HphI	GGTGA	5 cut(s)	67, 460, 605, 698, 728
Hpy166II	GTNNAC	4 cut(s)	71, 163, 659, 1708
Hpy188I	TCNGA	7 cut(s)	203, 213, 441, 681, 795, 1027, 1245
Hpy188III	TCNNGA	9 cut(s)	89, 622, 848, 1070, 1136, 1354, 1410, 1523, 1745
Hpy8I	GTNNAC	4 cut(s)	71, 163, 659, 1708
Hpy99I	CGWCG	4 cut(s)	161, 335, 408, 849
HpyAV	CCTTC	5 cut(s)	177, 795, 847, 1250, 1625
HpyCH4III	ACNGT	4 cut(s)	458, 869, 1080, 1363
HpyCH4IV	ACGT	4 cut(s)	159, 384, 773, 1681
HpyF10VI	GCNNNNNNNGC	5 cut(s)	230, 1526, 1729, 1844, 1853
HpyF3I	CTNAG	4 cut(s)	1099, 1242, 1295, 1882
HpySE526I	ACGT	4 cut(s)	159, 384, 773, 1681
Hsp92I	GRCGYC	1 cut(s)	820
HspAI	GCGC	2 cut(s)	602, 820
KasI	GGCGCC	1 cut(s)	819
Ksp22I	TGATCA	5 cut(s)	436, 477, 829, 1002, 1752
KspI	CCGCGG	1 cut(s)	397
LmnI	GCTCC	1 cut(s)	1793
Lsp1109I	GCAGC	7 cut(s)	688, 1027, 1741, 1859, 1952, 1955, 1975
LweI	GCATC	9 cut(s)	110, 432, 520, 751, 1143, 1236, 1333, 1514, 1909
MaeI	CTAG	3 cut(s)	948, 1253, 1328
MaeII	ACGT	4 cut(s)	159, 384, 773, 1681
MaeIII	GTNAC	2 cut(s)	262, 571
MflI	RGATCY	2 cut(s)	186, 1318
MhlI	GDGCHC	3 cut(s)	55, 661, 1612
MlsI	TGGCCA	1 cut(s)	311
MluCI	AATT	7 cut(s)	271, 364, 901, 1440, 1643, 1664, 2005
MluNI	TGGCCA	1 cut(s)	311
Mly113I	GGCGCC	1 cut(s)	820
MlyI	GAGTC	2 cut(s)	1174, 1648
Mox20I	TGGCCA	1 cut(s)	311
Mph1103I	ATGCAT	2 cut(s)	1507, 1606
MroXI	GAANNNNTTC	1 cut(s)	326
MscI	TGGCCA	1 cut(s)	311
MseI	TTAA	5 cut(s)	1124, 1230, 1598, 1647, 1859
MslI	CAYNNNNRTG	2 cut(s)	1081, 1824
Msp20I	TGGCCA	1 cut(s)	311
MspA1I	CMGCKG	5 cut(s)	396, 1008, 1348, 1850, 1946
MspI	CCGG	8 cut(s)	141, 285, 319, 378, 446, 576, 1636, 1923
MspR9I	CCNGG	8 cut(s)	141, 226, 286, 319, 379, 577, 1112, 1480
Mva1269I	GAATGC	5 cut(s)	568, 633, 1065, 1093, 1489
MvaI	CCWGG	3 cut(s)	226, 1112, 1480
MvnI	CGCG	2 cut(s)	396, 604
MwoI	GCNNNNNNNGC	5 cut(s)	230, 1526, 1729, 1844, 1853
NarI	GGCGCC	1 cut(s)	820
NciI	CCSGG	5 cut(s)	141, 286, 319, 379, 577
NdeI	CATATG	1 cut(s)	1020
NlaIV	GGNNCC	1 cut(s)	821
NmeAIII	GCCGAG	2 cut(s)	326, 796
NsiI	ATGCAT	2 cut(s)	1507, 1606
NspI	RCATGY	3 cut(s)	1336, 1608, 1988
PaeI	GCATGC	2 cut(s)	1336, 1988
PagI	TCATGA	2 cut(s)	1135, 1353
PctI	GAATGC	5 cut(s)	568, 633, 1065, 1093, 1489
PdmI	GAANNNNTTC	1 cut(s)	326
PfeI	GAWTC	2 cut(s)	7, 1339
PfoI	TCCNGGA	1 cut(s)	1478
PleI	GAGTC	2 cut(s)	1173, 1647
PluTI	GGCGCC	1 cut(s)	823
PpsI	GAGTC	2 cut(s)	1173, 1647
Ppu21I	YACGTR	1 cut(s)	1682
PsiI	TTATAA	2 cut(s)	768, 1397
Psp124BI	GAGCTC	1 cut(s)	55
Psp6I	CCWGG	3 cut(s)	224, 1110, 1478
PspEI	GGTNACC	1 cut(s)	571
PspGI	CCWGG	3 cut(s)	224, 1110, 1478
PspN4I	GGNNCC	1 cut(s)	821
PspPI	GGNCC	2 cut(s)	316, 1886
PstNI	CAGNNNCTG	1 cut(s)	1946
PsuI	RGATCY	2 cut(s)	186, 1318
PvuII	CAGCTG	3 cut(s)	1348, 1850, 1946
RsaI	GTAC	4 cut(s)	383, 488, 1306, 1904
RsaNI	GTAC	4 cut(s)	382, 487, 1305, 1903
RseI	CAYNNNNRTG	2 cut(s)	1081, 1824
SacI	GAGCTC	1 cut(s)	55
SacII	CCGCGG	1 cut(s)	397
SalI	GTCGAC	1 cut(s)	1706
SaqAI	TTAA	5 cut(s)	1124, 1230, 1598, 1647, 1859
Sau96I	GGNCC	2 cut(s)	316, 1886
ScaI	AGTACT	1 cut(s)	1306
SchI	GAGTC	2 cut(s)	1174, 1648
ScrFI	CCNGG	8 cut(s)	141, 226, 286, 319, 379, 577, 1112, 1480
SduI	GDGCHC	3 cut(s)	55, 661, 1612
SfaNI	GCATC	9 cut(s)	110, 432, 520, 751, 1143, 1236, 1333, 1514, 1909
SfcI	CTRYAG	2 cut(s)	584, 1993
SfoI	GGCGCC	1 cut(s)	821
Sfr303I	CCGCGG	1 cut(s)	397
SgrBI	CCGCGG	1 cut(s)	397
SinI	GGWCC	1 cut(s)	1886
SmiI	ATTTAAAT	1 cut(s)	1648
SmiMI	CAYNNNNRTG	2 cut(s)	1081, 1824
SmlI	CTYRAG	2 cut(s)	1521, 1733
SmoI	CTYRAG	2 cut(s)	1521, 1733
SphI	GCATGC	2 cut(s)	1336, 1988
Sse9I	AATT	7 cut(s)	271, 364, 901, 1440, 1643, 1664, 2005
SsiI	CCGC	8 cut(s)	394, 396, 520, 539, 632, 1008, 1157, 1316
SspDI	GGCGCC	1 cut(s)	819
SspI	AATATT	1 cut(s)	937
SspMI	CTAG	3 cut(s)	948, 1253, 1328
SstI	GAGCTC	1 cut(s)	55
StyD4I	CCNGG	8 cut(s)	139, 224, 284, 317, 377, 575, 1110, 1478
StyI	CCWWGG	1 cut(s)	312
SwaI	ATTTAAAT	1 cut(s)	1648
TaaI	ACNGT	4 cut(s)	458, 869, 1080, 1363
TaiI	ACGT	4 cut(s)	162, 387, 776, 1684
TaqI	TCGA	5 cut(s)	330, 528, 621, 1686, 1707
TasI	AATT	7 cut(s)	271, 364, 901, 1440, 1643, 1664, 2005
TatI	WGTACW	2 cut(s)	1304, 1902
TauI	GCSGC	6 cut(s)	396, 523, 541, 634, 1011, 1159
TfiI	GAWTC	2 cut(s)	7, 1339
Tru1I	TTAA	5 cut(s)	1124, 1230, 1598, 1647, 1859
Tru9I	TTAA	5 cut(s)	1124, 1230, 1598, 1647, 1859
TscAI	CASTG	4 cut(s)	180, 487, 874, 1083
TseI	GCWGC	7 cut(s)	701, 1040, 1729, 1847, 1940, 1943, 1988
TspGWI	ACGGA	2 cut(s)	268, 1878
TspRI	CASTG	4 cut(s)	180, 487, 874, 1083
VneI	GTGCAC	1 cut(s)	657
VpaK11BI	GGWCC	1 cut(s)	1886
XagI	CCTNNNNNAGG	1 cut(s)	1996
XapI	RAATTY	5 cut(s)	271, 364, 901, 1664, 2005
XceI	RCATGY	3 cut(s)	1336, 1608, 1988
XmiI	GTMKAC	3 cut(s)	70, 162, 1707
XmnI	GAANNNNTTC	1 cut(s)	326
XspI	CTAG	3 cut(s)	948, 1253, 1328
ZrmI	AGTACT	1 cut(s)	1306
Zsp2I	ATGCAT	2 cut(s)	1507, 1606

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.