Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000006

Orthogroup ID

OG0003199

Physical Location & Seq

Genomic Coordinates Forward (+)

16878939 .. 16886314

Gene Structure 7376 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug06G0122200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1968 bp

ATGAATTCCTTTGTGCTTTTTCAGTCCTCTTTGATTCCTTTGCTTTTGATCACTTCCTTTCTGTCGATCAATTTTCAATTAAGTGTGCGCGCCGATGATACAGTGGAATATGCCAACTGCAGCCAACCTATTACCTGCGGGAGCGCCACAAGCAACATCTCTTACCCCTTTTGGGGAGCGAACCTAGCCGAGTACTGTGGCAAATCTGGGTTTGAGGTCACATGCGAAGCCAACATCCCAATGATCACCATGAGGAATGTCAAGTTCAGAATTCTTGACATGAACAATAGCAGTACTACAACAACTCCTACTGTAAAAGTTGCTAGGGAGGATTACTGGGGAACTATCTGTCCCTCGACATATAATCCCACAAACCTCGACTTCTCTCTCTTCAGTTACTCTTCTGGGCTTCTGAACGTGACTTTTTGGTACGGATGCAATTCAACCGTAACAGTCACAGGTAATTCGCATGTCTGCAACAGTAGCATCGCTGCTTCCTATCTCACACAGACGAGAGCTAGTAATGTCCCAACTGATCCAGTTACTACGGGAGCCTGTCAATATAAGGTTTTAGTTCCGGTATTTGAATCCGCTTCTGAGGCTCTGGACAACAATGCTACAGATATTGAGACTGCGATAGATGGTGGTTTTGAATTGGATGTGCTAAATGCTGATTCTAGCCTGTGCACCAATTGCTTGTCATCAGGGGGAGTTTGTGGGCAAAACACTACTGTTGCCAACGAATTCAAGTGCTTTTGCCAGACTACTTCATCTTCGACAACCACATGTACTGAAAGCTCTTCACCATCTCCAAATTCAGCTCCATCCAATTCAGGTTCAATATCACGTGATTGGATCATAGGCATCTCGGCGGGTGTTTTTGGGGCCATTGTACTAATTGTTTGCGGTATATGCATATCAAGGAAAAGTTCTTTGGTCTTCTTCAAGAAACATGAAAAGGATGAGTTTGACATTGAAGCATTCATAAGAAACTGTGGATCGCTAAGCCCAAAGCGGTATCGTTATTCAGATTTGAAGAAAATGACAAACGCATTCAAAGATCAAATTGGTAAAGGTGGATATGGAATAGTGTACAAAGGAAAGCTACCTGATGGGCTCCTAGTCGCAGTGAAAGTGCTAAGTGAATTGAAGGGTAATGGAGAAGAATTTATCAATGAAGTTGCAAGCATTGGTAGAACCTCCCATGTCAATATAGTAAATCTTGTGGGATTCTGCTACGAAAGGGAGAAAAGAGCTCTCATATACGAGTACATGCCTAATGGATCACTAGATAAGTTTATGCATAATAAGCAAGGATCTTCTGATGAAAAGTGCCACTTGGAATGGAAAACATTATCTGAAATTGCAGTTGGGATTGCTCGAGGACTAGAGTATTTACACCGCGGTTGCAACACAAGAATTTTACATTTCGACATAAAGCCACAGAACATTCTTCTGGATAAAGATTTTTGCCCAAAGATATCTGATTTCGGCCTTGCTAAACTATGCATGACGAAGGAAAGTATTGTATCGATGCTAGGTACAAGAGGAACTGCAGGATACATTGCACCAGAAGTGTTTAGTCGGAACTTTGGAGGAGTATCTCATAAATCTGATGTTTATAGCTATGGGATGCTAGTTCTTGACATGGTTGGTGCAAGAAAGAATTTGGTTTCAGAAGTGTCTCATACCAGTGAAATGTTTCCACATTACATTTATAAAGATCTAGAGCTAGAGAAGAATGAGATTATATTTGGGGAAATAACAGAGGATGAGAGCAAAATAGCAAGAAAGATGATATTGATAAGTCTTGGGTGCATTCAGACCCTTCCATCTGATCGACCATCAATGACCAAAGTGGTAGAGATGTTGGAAGGACCTCTTCATTCCCTGCCAATTCCACCAAAGCCTCTCTTATGTTCTGAAGAAGAATCTGCACCCATGTCCCAACCATCTGAAACAGGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016553 GO:0016554 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR

Protein Analysis

655

Amino Acids

71.71

Weight (kDa)

5.54

Isoelectric Point (pI)

35.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GUB_WAK_bind	PF13947	40 - 100	3.4e-13	Wall-associated receptor kinase galacturonan-binding
WAK_assoc	PF14380	173 - 254	6.6e-12	Wall-associated receptor kinase C-terminal
PK_Tyr_Ser-Thr	PF07714	352 - 624	1.1e-43	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	353 - 622	2.3e-44	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003199)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G62260
fragaria_vesca	FvH4_2g07250
malus_domestica	MD10G1129300.v1.1
prunus_persica	Prupe.8G170100_v2.0.a1 Prupe.8G170100_v2.0.a1
pyrus_communis	pycom06g17110 pycom10g11090
rosa_chinensis	RchiOBHm_Chr6g0259851 RchiOBHm_Chr6g0279101
rosa_laevigata	RLG00000013168 RLG00000014504
rosa_multiflora	Rmu_co8522155.1_g000002 Rmu_sc0002080.1_g000004 Rmu_sc0009049.1_g000004 Rmu_sc0037609.1_g000001 Rmu_sc0037609.1_g000002 Rmu_sc0037609.1_g000003
rosa_roxburghii	Rroxscaffold_7G00189130 Rroxscaffold_7G00207040 Rroxscaffold_7G00207050 Rroxscaffold_7G00207060 Rroxscaffold_7G00207180 Rroxscaffold_7G00207190 Rroxscaffold_7G00207200
rosa_rugosa	Rorug05G0590700 Rorug05G0590800 Rorug06G0122200
rosa_samantha	Rh6AG107400 Rh6AG230900 Rh6BG102900 Rh6BG235800 Rh6DG090200 Rh6DG229500
rosa_wichuraiana	Rw0G019930 Rw0G021150 Rw6G009260 Rw6G020220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1719
Acc36I	ACCTGC	1 cut(s)	143
AccII	CGCG	2 cut(s)	90, 1404
AciI	CCGC	7 cut(s)	138, 591, 872, 906, 1015, 1402, 1404
AclWI	GGATC	5 cut(s)	530, 863, 1006, 1291, 1324
AcsI	RAATTY	7 cut(s)	4, 270, 743, 814, 1166, 1419, 1666
AcuI	CTGAAG	2 cut(s)	376, 1944
AcvI	CACGTG	1 cut(s)	848
AfaI	GTAC	8 cut(s)	194, 295, 431, 790, 894, 1094, 1271, 1543
AfiI	CCNNNNNNNGG	2 cut(s)	172, 173
AflIII	ACRYGT	1 cut(s)	785
AjuI	GAANNNNNNNTTGG	4 cut(s)	917, 949, 1353, 1385
AloI	GAACNNNNNNTCC	2 cut(s)	334, 366
AluBI	AGCT	7 cut(s)	518, 798, 821, 1105, 1256, 1626, 1732
AluI	AGCT	7 cut(s)	518, 798, 821, 1105, 1256, 1626, 1732
Alw21I	GWGCWC	2 cut(s)	689, 1258
Alw26I	GTCTC	2 cut(s)	623, 1689
Alw44I	GTGCAC	1 cut(s)	685
AlwI	GGATC	5 cut(s)	530, 863, 1006, 1291, 1324
Ama87I	CYCGRG	1 cut(s)	1380
AoxI	GGCC	2 cut(s)	885, 1492
ApaLI	GTGCAC	1 cut(s)	685
ApeKI	GCWGC	2 cut(s)	120, 491
ApoI	RAATTY	7 cut(s)	4, 270, 743, 814, 1166, 1419, 1666
Asp700I	GAANNNNTTC	1 cut(s)	1701
AspLEI	GCGC	3 cut(s)	90, 92, 146
AspS9I	GGNCC	2 cut(s)	885, 1877
AsuHPI	GGTGA	2 cut(s)	238, 795
AvaI	CYCGRG	1 cut(s)	1380
AvaII	GGWCC	1 cut(s)	1877
BaeGI	GKGCMC	1 cut(s)	689
BanII	GRGCYC	2 cut(s)	1119, 1258
BbrPI	CACGTG	1 cut(s)	848
BbsI	GAAGAC	1 cut(s)	931
Bbv12I	GWGCWC	2 cut(s)	689, 1258
BbvI	GCAGC	2 cut(s)	132, 478
BccI	CCATC	7 cut(s)	635, 814, 832, 1106, 1840, 1852, 1960
BciVI	GTATCC	1 cut(s)	1553
BclI	TGATCA	2 cut(s)	48, 243
BcoDI	GTCTC	2 cut(s)	623, 1689
BfmI	CTRYAG	3 cut(s)	118, 618, 1554
BfoI	RGCGCY	1 cut(s)	147
BfuAI	ACCTGC	1 cut(s)	143
BfuI	GTATCC	1 cut(s)	1553
BglII	AGATCT	1 cut(s)	1723
BisI	GCNGC	2 cut(s)	121, 492
BlpI	GCTNAGC	1 cut(s)	1004
BlsI	GCNGC	2 cut(s)	122, 493
BmcAI	AGTACT	2 cut(s)	194, 295
Bme18I	GGWCC	1 cut(s)	1877
BmeT110I	CYCGRG	1 cut(s)	1380
BmgT120I	GGNCC	2 cut(s)	885, 1877
BmiI	GGNNCC	3 cut(s)	553, 886, 1118
BmrI	ACTGGG	1 cut(s)	346
BmsI	GCATC	5 cut(s)	425, 495, 873, 1524, 1623
BmuI	ACTGGG	1 cut(s)	346
BpiI	GAAGAC	1 cut(s)	931
Bpu1102I	GCTNAGC	1 cut(s)	1004
Bsa29I	ATCGAT	1 cut(s)	1532
BsaAI	YACGTR	1 cut(s)	848
BsaJI	CCNNGG	1 cut(s)	1402
BsaWI	WCCGGW	1 cut(s)	577
BsaXI	ACNNNNNCTCC	2 cut(s)	289, 319
Bsc4I	CCNNNNNNNGG	2 cut(s)	172, 173
Bse1I	ACTGG	3 cut(s)	341, 539, 1692
Bse3DI	GCAATG	1 cut(s)	1563
BseCI	ATCGAT	1 cut(s)	1532
BseDI	CCNNGG	1 cut(s)	1402
BseGI	GGATG	7 cut(s)	234, 440, 664, 824, 967, 1638, 1777
BseLI	CCNNNNNNNGG	2 cut(s)	172, 173
BseMI	GCAATG	1 cut(s)	1563
BseMII	CTCAG	1 cut(s)	588
BseNI	ACTGG	3 cut(s)	341, 539, 1692
BsePI	GCGCGC	1 cut(s)	88
BseRI	GAGGAG	1 cut(s)	1611
BseSI	GKGCMC	1 cut(s)	689
BseXI	GCAGC	2 cut(s)	132, 478
BsgI	GTGCAG	1 cut(s)	1920
Bsh1236I	CGCG	2 cut(s)	90, 1404
BshFI	GGCC	2 cut(s)	887, 1494
BshVI	ATCGAT	1 cut(s)	1532
BsiHKAI	GWGCWC	2 cut(s)	689, 1258
BsiHKCI	CYCGRG	1 cut(s)	1380
BsiSI	CCGG	1 cut(s)	578
BslFI	GGGAC	3 cut(s)	336, 512, 1930
BslI	CCNNNNNNNGG	2 cut(s)	172, 173
BsmAI	GTCTC	2 cut(s)	623, 1689
BsmFI	GGGAC	3 cut(s)	336, 512, 1930
BsmI	GAATGC	3 cut(s)	980, 1052, 1818
BsnI	GGCC	2 cut(s)	887, 1494
BsoBI	CYCGRG	1 cut(s)	1380
Bsp1286I	GDGCHC	3 cut(s)	689, 1119, 1258
Bsp1407I	TGTACA	1 cut(s)	1092
Bsp1720I	GCTNAGC	1 cut(s)	1004
BspACI	CCGC	7 cut(s)	138, 591, 872, 906, 1015, 1402, 1404
BspANI	GGCC	2 cut(s)	887, 1494
BspCNI	CTCAG	1 cut(s)	589
BspDI	ATCGAT	1 cut(s)	1532
BspFNI	CGCG	2 cut(s)	90, 1404
BspLI	GGNNCC	3 cut(s)	553, 886, 1118
BspMAI	CTGCAG	2 cut(s)	122, 1558
BspMI	ACCTGC	1 cut(s)	143
BspPI	GGATC	5 cut(s)	530, 863, 1006, 1291, 1324
BspQI	GCTCTTC	1 cut(s)	805
BsrDI	GCAATG	1 cut(s)	1563
BsrGI	TGTACA	1 cut(s)	1092
BsrI	ACTGG	3 cut(s)	341, 539, 1692
BssECI	CCNNGG	1 cut(s)	1402
BssHII	GCGCGC	1 cut(s)	88
Bst4CI	ACNGT	8 cut(s)	103, 197, 313, 448, 454, 482, 733, 995
Bst6I	CTCTTC	4 cut(s)	395, 406, 805, 1887
BstAPI	GCANNNNNTGC	1 cut(s)	693
BstAUI	TGTACA	1 cut(s)	1092
BstBAI	YACGTR	1 cut(s)	848
BstC8I	GCNNGC	2 cut(s)	90, 1186
BstDEI	CTNAG	3 cut(s)	597, 1004, 1139
BstDSI	CCRYGG	1 cut(s)	1402
BstF5I	GGATG	7 cut(s)	234, 440, 664, 824, 967, 1638, 1777
BstFNI	CGCG	2 cut(s)	90, 1404
BstH2I	RGCGCY	1 cut(s)	147
BstHHI	GCGC	3 cut(s)	90, 92, 146
BstMAI	GTCTC	2 cut(s)	623, 1689
BstMWI	GCNNNNNNNGC	7 cut(s)	150, 185, 483, 599, 693, 912, 1309
BstNSI	RCATGY	4 cut(s)	225, 473, 789, 1276
BstSFI	CTRYAG	3 cut(s)	118, 618, 1554
BstSLI	GKGCMC	1 cut(s)	689
BstUI	CGCG	2 cut(s)	90, 1404
BstV1I	GCAGC	2 cut(s)	132, 478
BstV2I	GAAGAC	1 cut(s)	931
BstX2I	RGATCY	2 cut(s)	1316, 1723
BstYI	RGATCY	2 cut(s)	1316, 1723
Bsu15I	ATCGAT	1 cut(s)	1532
BsuI	GTATCC	1 cut(s)	1553
BsuRI	GGCC	2 cut(s)	887, 1494
BsuTUI	ATCGAT	1 cut(s)	1532
BtgI	CCRYGG	1 cut(s)	1402
BtgZI	GCGATG	1 cut(s)	472
BtsCI	GGATG	7 cut(s)	234, 440, 664, 824, 967, 1638, 1777
BtsI	GCAGTG	1 cut(s)	1134
BtsIMutI	CAGTG	3 cut(s)	108, 1134, 1699
BveI	ACCTGC	1 cut(s)	143
Cac8I	GCNNGC	2 cut(s)	90, 1186
CfoI	GCGC	3 cut(s)	90, 92, 146
Cfr13I	GGNCC	2 cut(s)	885, 1877
Cfr42I	CCGCGG	1 cut(s)	1405
ClaI	ATCGAT	1 cut(s)	1532
Csp6I	GTAC	8 cut(s)	193, 294, 430, 789, 893, 1093, 1270, 1542
CviQI	GTAC	8 cut(s)	193, 294, 430, 789, 893, 1093, 1270, 1542
DdeI	CTNAG	3 cut(s)	597, 1004, 1139
Eam1104I	CTCTTC	4 cut(s)	395, 406, 805, 1887
EarI	CTCTTC	4 cut(s)	395, 406, 805, 1887
Ecl136II	GAGCTC	1 cut(s)	1256
Eco24I	GRGCYC	2 cut(s)	1119, 1258
Eco32I	GATATC	1 cut(s)	1482
Eco47I	GGWCC	1 cut(s)	1877
Eco53kI	GAGCTC	1 cut(s)	1256
Eco57I	CTGAAG	2 cut(s)	376, 1944
Eco72I	CACGTG	1 cut(s)	848
Eco88I	CYCGRG	1 cut(s)	1380
EcoICRI	GAGCTC	1 cut(s)	1256
EcoO109I	RGGNCCY	1 cut(s)	1877
EcoRI	GAATTC	3 cut(s)	4, 270, 743
EcoRV	GATATC	1 cut(s)	1482
EcoT22I	ATGCAT	3 cut(s)	917, 1305, 1511
EcoT38I	GRGCYC	2 cut(s)	1119, 1258
FalI	AAGNNNNNCTT	6 cut(s)	1322, 1354, 1866, 1898, 1898, 1930
FaqI	GGGAC	3 cut(s)	336, 512, 1930
FauI	CCCGC	2 cut(s)	131, 865
FbaI	TGATCA	2 cut(s)	48, 243
Fnu4HI	GCNGC	2 cut(s)	121, 492
FokI	GGATG	7 cut(s)	221, 447, 671, 811, 974, 1645, 1784
FriOI	GRGCYC	2 cut(s)	1119, 1258
Fsp4HI	GCNGC	2 cut(s)	121, 492
GlaI	GCGC	3 cut(s)	89, 91, 145
GluI	GCNGC	2 cut(s)	121, 492
HaeII	RGCGCY	1 cut(s)	147
HaeIII	GGCC	2 cut(s)	887, 1494
HapII	CCGG	1 cut(s)	578
HhaI	GCGC	3 cut(s)	90, 92, 146
Hin6I	GCGC	3 cut(s)	88, 90, 144
HinP1I	GCGC	3 cut(s)	88, 90, 144
HinfI	GANTC	5 cut(s)	34, 587, 674, 1230, 1931
HpaII	CCGG	1 cut(s)	578
HphI	GGTGA	2 cut(s)	238, 795
Hpy166II	GTNNAC	2 cut(s)	687, 1093
Hpy188III	TCNNGA	6 cut(s)	275, 605, 946, 1457, 1643, 1727
Hpy8I	GTNNAC	2 cut(s)	687, 1093
HpyAV	CCTTC	4 cut(s)	1144, 1510, 1838, 1868
HpyCH4III	ACNGT	8 cut(s)	103, 197, 313, 448, 454, 482, 733, 995
HpyCH4IV	ACGT	2 cut(s)	417, 847
HpyF10VI	GCNNNNNNNGC	7 cut(s)	150, 185, 483, 599, 693, 912, 1309
HpyF3I	CTNAG	3 cut(s)	597, 1004, 1139
HpySE526I	ACGT	2 cut(s)	417, 847
HspAI	GCGC	3 cut(s)	88, 90, 144
Ksp22I	TGATCA	2 cut(s)	48, 243
KspI	CCGCGG	1 cut(s)	1405
LguI	GCTCTTC	1 cut(s)	805
LmnI	GCTCC	5 cut(s)	141, 176, 551, 826, 1122
Lsp1109I	GCAGC	2 cut(s)	132, 478
LweI	GCATC	5 cut(s)	425, 495, 873, 1524, 1623
MaeII	ACGT	2 cut(s)	417, 847
MaeIII	GTNAC	6 cut(s)	217, 395, 418, 448, 454, 541
MfeI	CAATTG	1 cut(s)	691
MflI	RGATCY	2 cut(s)	1316, 1723
MhlI	GDGCHC	3 cut(s)	689, 1119, 1258
MmeI	TCCRAC	2 cut(s)	1565, 1851
Mph1103I	ATGCAT	3 cut(s)	917, 1305, 1511
MroXI	GAANNNNTTC	1 cut(s)	1701
MseI	TTAA	1 cut(s)	80
MslI	CAYNNNNRTG	2 cut(s)	239, 1692
MspA1I	CMGCKG	1 cut(s)	1404
MspI	CCGG	1 cut(s)	578
MunI	CAATTG	1 cut(s)	691
Mva1269I	GAATGC	3 cut(s)	980, 1052, 1818
MvnI	CGCG	2 cut(s)	90, 1404
MwoI	GCNNNNNNNGC	7 cut(s)	150, 185, 483, 599, 693, 912, 1309
NlaIV	GGNNCC	3 cut(s)	553, 886, 1118
NmeAIII	GCCGAG	2 cut(s)	214, 848
NmuCI	GTSAC	3 cut(s)	217, 418, 454
NsiI	ATGCAT	3 cut(s)	917, 1305, 1511
NspI	RCATGY	4 cut(s)	225, 473, 789, 1276
PaeR7I	CTCGAG	1 cut(s)	1380
PauI	GCGCGC	1 cut(s)	88
PciI	ACATGT	1 cut(s)	785
PciSI	GCTCTTC	1 cut(s)	805
PctI	GAATGC	3 cut(s)	980, 1052, 1818
PdmI	GAANNNNTTC	1 cut(s)	1701
PfeI	GAWTC	5 cut(s)	34, 587, 674, 1230, 1931
PkrI	GCNGC	2 cut(s)	122, 493
PmaCI	CACGTG	1 cut(s)	848
PmlI	CACGTG	1 cut(s)	848
Ppu21I	YACGTR	1 cut(s)	848
PpuMI	RGGWCCY	1 cut(s)	1877
PscI	ACATGT	1 cut(s)	785
PsiI	TTATAA	1 cut(s)	1719
Psp124BI	GAGCTC	1 cut(s)	1258
Psp5II	RGGWCCY	1 cut(s)	1877
PspCI	CACGTG	1 cut(s)	848
PspN4I	GGNNCC	3 cut(s)	553, 886, 1118
PspPI	GGNCC	2 cut(s)	885, 1877
PspPPI	RGGWCCY	1 cut(s)	1877
PspXI	VCTCGAGB	1 cut(s)	1380
PstI	CTGCAG	2 cut(s)	122, 1558
PsuI	RGATCY	2 cut(s)	1316, 1723
PteI	GCGCGC	1 cut(s)	88
RsaI	GTAC	8 cut(s)	194, 295, 431, 790, 894, 1094, 1271, 1543
RsaNI	GTAC	8 cut(s)	193, 294, 430, 789, 893, 1093, 1270, 1542
RseI	CAYNNNNRTG	2 cut(s)	239, 1692
SacI	GAGCTC	1 cut(s)	1258
SacII	CCGCGG	1 cut(s)	1405
SapI	GCTCTTC	1 cut(s)	805
SaqAI	TTAA	1 cut(s)	80
SatI	GCNGC	2 cut(s)	121, 492
Sau96I	GGNCC	2 cut(s)	885, 1877
ScaI	AGTACT	2 cut(s)	194, 295
SduI	GDGCHC	3 cut(s)	689, 1119, 1258
SfaNI	GCATC	5 cut(s)	425, 495, 873, 1524, 1623
SfcI	CTRYAG	3 cut(s)	118, 618, 1554
Sfr274I	CTCGAG	1 cut(s)	1380
Sfr303I	CCGCGG	1 cut(s)	1405
SgrBI	CCGCGG	1 cut(s)	1405
SinI	GGWCC	1 cut(s)	1877
SlaI	CTCGAG	1 cut(s)	1380
SmiMI	CAYNNNNRTG	2 cut(s)	239, 1692
SmlI	CTYRAG	1 cut(s)	1380
SmoI	CTYRAG	1 cut(s)	1380
SsiI	CCGC	7 cut(s)	138, 591, 872, 906, 1015, 1402, 1404
SstI	GAGCTC	1 cut(s)	1258
TaaI	ACNGT	8 cut(s)	103, 197, 313, 448, 454, 482, 733, 995
TaiI	ACGT	2 cut(s)	420, 850
TaqI	TCGA	8 cut(s)	65, 356, 378, 776, 1381, 1431, 1532, 1840
TatI	WGTACW	6 cut(s)	192, 293, 788, 892, 1092, 1269
TfiI	GAWTC	5 cut(s)	34, 587, 674, 1230, 1931
Tru1I	TTAA	1 cut(s)	80
Tru9I	TTAA	1 cut(s)	80
TscAI	CASTG	3 cut(s)	108, 1134, 1699
TseFI	GTSAC	3 cut(s)	217, 418, 454
TseI	GCWGC	2 cut(s)	120, 491
Tsp45I	GTSAC	3 cut(s)	217, 418, 454
TspDTI	ATGAA	8 cut(s)	17, 296, 759, 969, 973, 1191, 1341, 1874
TspGWI	ACGGA	1 cut(s)	447
TspRI	CASTG	3 cut(s)	108, 1134, 1699
VneI	GTGCAC	1 cut(s)	685
VpaK11BI	GGWCC	1 cut(s)	1877
XapI	RAATTY	7 cut(s)	4, 270, 743, 814, 1166, 1419, 1666
XbaI	TCTAGA	1 cut(s)	1726
XceI	RCATGY	4 cut(s)	225, 473, 789, 1276
XhoI	CTCGAG	1 cut(s)	1380
XmnI	GAANNNNTTC	1 cut(s)	1701
ZrmI	AGTACT	2 cut(s)	194, 295
Zsp2I	ATGCAT	3 cut(s)	917, 1305, 1511

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.