Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0003199

Physical Location & Seq

Genomic Coordinates Reverse (-)

40494528 .. 40496544

Gene Structure 2017 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G020220.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1986 bp

ATGATGATTCTGGGTAATGCAAATGGTTTCATATATAAACTCATGCCCCGAGCTCGTATTATTCTTCGTCTACTCACCATCCCATTACCCAATAATCACGCATCCCCACAGCACTCTTGTTTTGCCTCAACTTCAAAACCCGGCAGTCATGCCAAAGACGTAGACCTTTTTTCACTGAACAGAAGGATCTCCCATTTGATCCGAACTGGTCAGATAGAGCAAGCCAGGGAAGCCTTTGACAAGATGAAGCGACGGAATATCGTTACTTGGAATTCAATGATAACCGGGTATGTGAAACGCCGAGAGATGGCCAAGGCCCGGAAGCTGTTCGACGAAATGCCTGACAGAGATGTTGTGTCCTGGAATTTGATGATATCCGGGTACGTTTCGTGCCGCGGAAGTCGTCGGCATGTTGAGGAGGGCAGGACTTTGTTTGATCAGATGCCGGTGAGGGACTGTGTTTCTTGGAACACGATGATCAGTGGGTACGCCAAGAATGGGAGGATGGGGGAGGCATTGCGGCTTTTCGATGCCATGCCGCAGAGGAGTGTTGTTTCTTGGAATGCAATGGTTACCGGGTTTCTACAGAATGGCCATGTTGCGCGTGCGATTGAGTTTTTCGAGAGAATGCCGCAGAGGGATGGGGCTTCCTTAGTGCACTCGTGTCGGGGCTTATTCAGAATGGTGAGCTGGATGAAGCTGCGAGGATTGTTGGTGAATGTGGGAAGAGGGATGAGGAGGGGGTGGAAGATTTGGTGGATGCTTAATACGTTGGTTGCTGGTTATGGTCAGAGAGGAAGGGTTGAAGAGGCTCGGCGCCTTTTTGATCAAATACCATTTCGTCGTGAGAAGGGGAATGAGGACAGTGGGAGGTTTGAGAGGAATGTATCGTGGAATTCTATGATTATGTGCTATGTGAAAACAGGAAATATTGTTTCTGCTAGAGAACTTTTTGACCAAATGATAGAACGAGATACCTTTTCGTGGAATACCATGATCAGCGGCTATGTTCATATGTCAGATATGGAAGAGGCTGCCAATCTCTTTCGTAGAATGCCAAATCCTGATACACTGTCATGGAATGCAATGATCTTAGGGTATGCCCAGGTTGGGCGTTTAAAACTGGCTCATGATTTTTTTGAGAGGATGCCGCCACAAGGGTATGAGAAAAATGAGGACTTTATGGGTGGAGTTAAGCTTTTCTCTCAGATGCAACTAGAAGGAGAGAAACCTGATAGACACACTTTATCATCAGTTCTTAGTGTGAGTACTGGGTTAGCGGATCTGCATCTAGGCATGCAGATTCATCAGCTGGTCATGAAGACAGTTATTGCAGATTTGCCAATAAACAACTCTCTTATAACTATGTATTCACGATGTGGTGCTATCAAAGAGGCAGAAACAATTTTTGATGAGATGAAGCAGCAGAAAGATGTGATCTCCTGGAATGCAATGATTGGGGGCTATGCATCTCATGGTTTTGCTGCAGAGGCTTTAGGACTTTTTTGTTTGATGAAGAGGTCAAAGGTGCAACCTACATATATAACATTTATTGCAGTTTTAAATGCATGTGCTCATGCAGGATTAGTTGAAGAAGGCCGGAGTCAATTTAAATCCATGATTAGTGAATTTGGCATTGAGCCACGTATCGAACACTATGCCTCCCTTGTCGACATCATTGGTCGGCATGGGCAGCTTGAGGAGGCTCACGATTTGATCAATAGTATGGCATTTGAACCTGATAAGGCTGTGTGGGGAGCATTATTAGGTGCTTGTAGAGTGCATAACAATGTAGAGTTGGCAAAAGTTGCAGCTGAAGCATTAACAAGACTTGAACCTGAAAGCTCAGGTCCGTATGTATTGTTGTACAATATGTATGCTGATGCCGGACTATGGGATGAAGCAGCAGCTGTGAGATCGCTGATGGATGAAAACAAGATAACAAAGCATGCTGCCTATAGTAGGGTAAATTCTTCGCATTGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016553 GO:0016554 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR

Protein Analysis

661

Amino Acids

75.13

Weight (kDa)

9.12

Isoelectric Point (pI)

46.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	85 - 117	4.8e-09	PPR repeat family
PPR	PF01535	88 - 117	5.3e-08	PPR repeat
PPR_1	PF12854	151 - 179	1.6e-08	PPR repeat
PPR_2	PF13041	152 - 184	1.5e-08	PPR repeat family
PPR	PF01535	154 - 182	1.2e-09	PPR repeat
PPR	PF01535	296 - 324	5.5e-08	PPR repeat
PPR	PF01535	327 - 352	1.5e-06	PPR repeat
PPR_2	PF13041	477 - 526	7.2e-10	PPR repeat family
PPR	PF01535	480 - 509	4.6e-07	PPR repeat
E_motif	PF20431	595 - 654	5.5e-13	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0003199)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G62260
fragaria_vesca	FvH4_2g07250
malus_domestica	MD10G1129300.v1.1
prunus_persica	Prupe.8G170100_v2.0.a1 Prupe.8G170100_v2.0.a1
pyrus_communis	pycom06g17110 pycom10g11090
rosa_chinensis	RchiOBHm_Chr6g0259851 RchiOBHm_Chr6g0279101
rosa_laevigata	RLG00000013168 RLG00000014504
rosa_multiflora	Rmu_co8522155.1_g000002 Rmu_sc0002080.1_g000004 Rmu_sc0009049.1_g000004 Rmu_sc0037609.1_g000001 Rmu_sc0037609.1_g000002 Rmu_sc0037609.1_g000003
rosa_roxburghii	Rroxscaffold_7G00189130 Rroxscaffold_7G00207040 Rroxscaffold_7G00207050 Rroxscaffold_7G00207060 Rroxscaffold_7G00207180 Rroxscaffold_7G00207190 Rroxscaffold_7G00207200
rosa_rugosa	Rorug05G0590700 Rorug05G0590800 Rorug06G0122200
rosa_samantha	Rh6AG107400 Rh6AG230900 Rh6BG102900 Rh6BG235800 Rh6DG090200 Rh6DG229500
rosa_wichuraiana	Rw0G019930 Rw0G021150 Rw6G009260 Rw6G020220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1361
AccB1I	GGYRCC	1 cut(s)	816
AccI	GTMKAC	3 cut(s)	70, 162, 1671
AccII	CGCG	2 cut(s)	396, 604
AciI	CCGC	8 cut(s)	394, 396, 520, 539, 632, 1002, 1151, 1280
AclWI	GGATC	3 cut(s)	193, 194, 1290
AcoI	YGGCCR	2 cut(s)	309, 592
AcsI	RAATTY	5 cut(s)	271, 364, 895, 1628, 1969
AcuI	CTGAAG	1 cut(s)	1836
AcyI	GRCGYC	1 cut(s)	817
AdeI	CACNNNGTG	1 cut(s)	1379
AfaI	GTAC	4 cut(s)	383, 488, 1270, 1868
AfiI	CCNNNNNNNGG	8 cut(s)	307, 318, 498, 984, 1110, 1157, 1893, 1962
AgsI	TTSAA	6 cut(s)	135, 276, 806, 1592, 1736, 1835
AjnI	CCWGG	4 cut(s)	224, 359, 1104, 1442
Alw21I	GWGCWC	3 cut(s)	55, 660, 1576
Alw44I	GTGCAC	1 cut(s)	656
AlwI	GGATC	3 cut(s)	193, 194, 1290
AlwNI	CAGNNNCTG	1 cut(s)	1910
Ama87I	CYCGRG	1 cut(s)	48
AoxI	GGCC	4 cut(s)	309, 315, 592, 1597
ApaLI	GTGCAC	1 cut(s)	656
ApeKI	GCWGC	9 cut(s)	700, 1034, 1423, 1484, 1693, 1811, 1904, 1907, 1952
ApoI	RAATTY	5 cut(s)	271, 364, 895, 1628, 1969
Asp700I	GAANNNNTTC	1 cut(s)	326
AspLEI	GCGC	2 cut(s)	604, 819
AspS9I	GGNCC	2 cut(s)	316, 1850
AsuC2I	CCSGG	5 cut(s)	141, 286, 319, 379, 577
AsuHPI	GGTGA	4 cut(s)	67, 460, 697, 727
AvaI	CYCGRG	1 cut(s)	48
AvaII	GGWCC	1 cut(s)	1850
BaeGI	GKGCMC	1 cut(s)	660
BalI	TGGCCA	2 cut(s)	311, 594
BanI	GGYRCC	1 cut(s)	816
BanII	GRGCYC	1 cut(s)	55
BauI	CACGAG	1 cut(s)	661
BbsI	GAAGAC	1 cut(s)	1328
Bbv12I	GWGCWC	3 cut(s)	55, 660, 1576
BbvI	GCAGC	9 cut(s)	687, 1021, 1435, 1471, 1705, 1823, 1916, 1919, 1939
BccI	CCATC	5 cut(s)	86, 301, 499, 635, 1918
BcgI	CGANNNNNNTGC	4 cut(s)	647, 681, 1640, 1674
BciT130I	CCWGG	4 cut(s)	226, 361, 1106, 1444
BclI	TGATCA	5 cut(s)	436, 477, 826, 996, 1716
BcnI	CCSGG	5 cut(s)	141, 286, 319, 379, 577
BfaI	CTAG	3 cut(s)	942, 1217, 1292
BfmI	CTRYAG	3 cut(s)	584, 1485, 1957
BfoI	RGCGCY	1 cut(s)	820
BmcAI	AGTACT	1 cut(s)	1270
Bme1390I	CCNGG	9 cut(s)	141, 226, 286, 319, 361, 379, 577, 1106, 1444
Bme18I	GGWCC	1 cut(s)	1850
BmeT110I	CYCGRG	1 cut(s)	48
BmgT120I	GGNCC	2 cut(s)	316, 1850
BmiI	GGNNCC	1 cut(s)	818
BmrFI	CCNGG	9 cut(s)	141, 226, 286, 319, 361, 379, 577, 1106, 1444
BmrI	ACTGGG	1 cut(s)	1281
BmsI	GCATC	9 cut(s)	110, 432, 520, 750, 1137, 1200, 1297, 1478, 1873
BmuI	ACTGGG	1 cut(s)	1281
BpiI	GAAGAC	1 cut(s)	1328
Bpu10I	CCTNAGC	1 cut(s)	1846
BpuEI	CTTGAG	1 cut(s)	1718
BpuMI	CCSGG	5 cut(s)	141, 286, 319, 379, 577
BsaAI	YACGTR	1 cut(s)	1646
BsaBI	GATNNNNATC	1 cut(s)	1287
BsaHI	GRCGYC	1 cut(s)	817
BsaJI	CCNNGG	4 cut(s)	225, 312, 394, 1104
BsaXI	ACNNNNNCTCC	2 cut(s)	1646, 1676
Bsc4I	CCNNNNNNNGG	8 cut(s)	307, 318, 498, 984, 1110, 1157, 1893, 1962
Bse118I	RCCGGY	1 cut(s)	445
Bse1I	ACTGG	3 cut(s)	211, 1128, 1276
Bse3DI	GCAATG	5 cut(s)	515, 573, 1092, 1458, 1978
Bse8I	GATNNNNATC	1 cut(s)	1287
BseBI	CCWGG	4 cut(s)	226, 361, 1106, 1444
BseDI	CCNNGG	4 cut(s)	225, 312, 394, 1104
BseJI	GATNNNNATC	1 cut(s)	1287
BseLI	CCNNNNNNNGG	8 cut(s)	307, 318, 498, 984, 1110, 1157, 1893, 1962
BseMI	GCAATG	5 cut(s)	515, 573, 1092, 1458, 1978
BseMII	CTCAG	2 cut(s)	1220, 1860
BseNI	ACTGG	3 cut(s)	211, 1128, 1276
BseRI	GAGGAG	4 cut(s)	431, 559, 751, 1715
BseSI	GKGCMC	1 cut(s)	660
BseXI	GCAGC	9 cut(s)	687, 1021, 1435, 1471, 1705, 1823, 1916, 1919, 1939
Bsh1236I	CGCG	2 cut(s)	396, 604
BshFI	GGCC	4 cut(s)	311, 317, 594, 1599
BshNI	GGYRCC	1 cut(s)	816
BsiHKAI	GWGCWC	3 cut(s)	55, 660, 1576
BsiHKCI	CYCGRG	1 cut(s)	48
BsiSI	CCGG	8 cut(s)	141, 285, 319, 378, 446, 576, 1600, 1887
BslFI	GGGAC	1 cut(s)	467
BslI	CCNNNNNNNGG	8 cut(s)	307, 318, 498, 984, 1110, 1157, 1893, 1962
BsmFI	GGGAC	1 cut(s)	467
BsmI	GAATGC	5 cut(s)	568, 633, 1059, 1087, 1453
BsnI	GGCC	4 cut(s)	311, 317, 594, 1599
BsoBI	CYCGRG	1 cut(s)	48
Bsp1286I	GDGCHC	3 cut(s)	55, 660, 1576
Bsp1407I	TGTACA	1 cut(s)	1866
BspACI	CCGC	8 cut(s)	394, 396, 520, 539, 632, 1002, 1151, 1280
BspANI	GGCC	4 cut(s)	311, 317, 594, 1599
BspCNI	CTCAG	2 cut(s)	1219, 1859
BspFNI	CGCG	2 cut(s)	396, 604
BspHI	TCATGA	2 cut(s)	1129, 1317
BspLI	GGNNCC	1 cut(s)	818
BspMAI	CTGCAG	1 cut(s)	1489
BspPI	GGATC	3 cut(s)	193, 194, 1290
BspT107I	GGYRCC	1 cut(s)	816
BsrDI	GCAATG	5 cut(s)	515, 573, 1092, 1458, 1978
BsrFI	RCCGGY	1 cut(s)	445
BsrGI	TGTACA	1 cut(s)	1866
BsrI	ACTGG	3 cut(s)	211, 1128, 1276
BssAI	RCCGGY	1 cut(s)	445
BssECI	CCNNGG	4 cut(s)	225, 312, 394, 1104
BssNI	GRCGYC	1 cut(s)	817
BssSI	CACGAG	1 cut(s)	661
BssT1I	CCWWGG	1 cut(s)	312
Bst2BI	CACGAG	1 cut(s)	661
Bst2UI	CCWGG	4 cut(s)	226, 361, 1106, 1444
Bst4CI	ACNGT	4 cut(s)	458, 866, 1074, 1327
Bst6I	CTCTTC	4 cut(s)	721, 801, 1023, 1511
BstACI	GRCGYC	1 cut(s)	817
BstAPI	GCANNNNNTGC	1 cut(s)	1808
BstAUI	TGTACA	1 cut(s)	1866
BstBAI	YACGTR	1 cut(s)	1646
BstC8I	GCNNGC	4 cut(s)	222, 606, 1298, 1950
BstDEI	CTNAG	5 cut(s)	652, 1093, 1206, 1259, 1846
BstDSI	CCRYGG	1 cut(s)	394
BstEII	GGTNACC	1 cut(s)	571
BstENI	CCTNNNNNAGG	1 cut(s)	1960
BstFNI	CGCG	2 cut(s)	396, 604
BstH2I	RGCGCY	1 cut(s)	820
BstHHI	GCGC	2 cut(s)	604, 819
BstMWI	GCNNNNNNNGC	5 cut(s)	230, 1490, 1693, 1808, 1817
BstNI	CCWGG	4 cut(s)	226, 361, 1106, 1444
BstNSI	RCATGY	4 cut(s)	413, 1300, 1572, 1952
BstPI	GGTNACC	1 cut(s)	571
BstSCI	CCNGG	9 cut(s)	139, 224, 284, 317, 359, 377, 575, 1104, 1442
BstSFI	CTRYAG	3 cut(s)	584, 1485, 1957
BstSLI	GKGCMC	1 cut(s)	660
BstUI	CGCG	2 cut(s)	396, 604
BstV1I	GCAGC	9 cut(s)	687, 1021, 1435, 1471, 1705, 1823, 1916, 1919, 1939
BstV2I	GAAGAC	1 cut(s)	1328
BstX2I	RGATCY	2 cut(s)	186, 1282
BstYI	RGATCY	2 cut(s)	186, 1282
BsuRI	GGCC	4 cut(s)	311, 317, 594, 1599
BtgI	CCRYGG	1 cut(s)	394
BtsIMutI	CAGTG	4 cut(s)	173, 487, 871, 1070
Cac8I	GCNNGC	4 cut(s)	222, 606, 1298, 1950
CaiI	CAGNNNCTG	1 cut(s)	1910
CciI	TCATGA	2 cut(s)	1129, 1317
CfoI	GCGC	2 cut(s)	604, 819
Cfr10I	RCCGGY	1 cut(s)	445
Cfr13I	GGNCC	2 cut(s)	316, 1850
Cfr42I	CCGCGG	1 cut(s)	397
Csp6I	GTAC	4 cut(s)	382, 487, 1269, 1867
CviQI	GTAC	4 cut(s)	382, 487, 1269, 1867
DdeI	CTNAG	5 cut(s)	652, 1093, 1206, 1259, 1846
DinI	GGCGCC	1 cut(s)	818
DraI	TTTAAA	3 cut(s)	1119, 1563, 1612
DraIII	CACNNNGTG	1 cut(s)	1379
EaeI	YGGCCR	2 cut(s)	309, 592
Eam1104I	CTCTTC	4 cut(s)	721, 801, 1023, 1511
EarI	CTCTTC	4 cut(s)	721, 801, 1023, 1511
Ecl136II	GAGCTC	1 cut(s)	53
Eco130I	CCWWGG	1 cut(s)	312
Eco24I	GRGCYC	1 cut(s)	55
Eco32I	GATATC	1 cut(s)	375
Eco47I	GGWCC	1 cut(s)	1850
Eco53kI	GAGCTC	1 cut(s)	53
Eco57I	CTGAAG	1 cut(s)	1836
Eco88I	CYCGRG	1 cut(s)	48
Eco91I	GGTNACC	1 cut(s)	571
EcoICRI	GAGCTC	1 cut(s)	53
EcoNI	CCTNNNNNAGG	1 cut(s)	1960
EcoO65I	GGTNACC	1 cut(s)	571
EcoRI	GAATTC	2 cut(s)	271, 895
EcoRII	CCWGG	4 cut(s)	224, 359, 1104, 1442
EcoRV	GATATC	1 cut(s)	375
EcoT14I	CCWWGG	1 cut(s)	312
EcoT22I	ATGCAT	2 cut(s)	1471, 1570
EcoT38I	GRGCYC	1 cut(s)	55
EgeI	GGCGCC	1 cut(s)	818
EheI	GGCGCC	1 cut(s)	818
ErhI	CCWWGG	1 cut(s)	312
FaqI	GGGAC	1 cut(s)	467
FauNDI	CATATG	1 cut(s)	1014
FbaI	TGATCA	5 cut(s)	436, 477, 826, 996, 1716
FblI	GTMKAC	3 cut(s)	70, 162, 1671
FriOI	GRGCYC	1 cut(s)	55
FspBI	CTAG	3 cut(s)	942, 1217, 1292
GlaI	GCGC	2 cut(s)	603, 818
HaeII	RGCGCY	1 cut(s)	820
HaeIII	GGCC	4 cut(s)	311, 317, 594, 1599
HapII	CCGG	8 cut(s)	141, 285, 319, 378, 446, 576, 1600, 1887
HhaI	GCGC	2 cut(s)	604, 819
Hin1I	GRCGYC	1 cut(s)	817
Hin6I	GCGC	2 cut(s)	602, 817
HinP1I	GCGC	2 cut(s)	602, 817
HincII	GTYRAC	1 cut(s)	1672
HindII	GTYRAC	1 cut(s)	1672
HindIII	AAGCTT	1 cut(s)	1196
HinfI	GANTC	3 cut(s)	7, 1303, 1603
HpaII	CCGG	8 cut(s)	141, 285, 319, 378, 446, 576, 1600, 1887
HphI	GGTGA	4 cut(s)	67, 460, 697, 727
Hpy166II	GTNNAC	4 cut(s)	71, 163, 658, 1672
Hpy188I	TCNGA	7 cut(s)	203, 213, 441, 680, 792, 1021, 1209
Hpy188III	TCNNGA	7 cut(s)	622, 845, 1064, 1130, 1318, 1374, 1709
Hpy8I	GTNNAC	4 cut(s)	71, 163, 658, 1672
Hpy99I	CGWCG	4 cut(s)	255, 335, 408, 846
HpyAV	CCTTC	5 cut(s)	177, 792, 844, 1214, 1589
HpyCH4III	ACNGT	4 cut(s)	458, 866, 1074, 1327
HpyCH4IV	ACGT	4 cut(s)	159, 384, 770, 1645
HpyF10VI	GCNNNNNNNGC	5 cut(s)	230, 1490, 1693, 1808, 1817
HpyF3I	CTNAG	5 cut(s)	652, 1093, 1206, 1259, 1846
HpySE526I	ACGT	4 cut(s)	159, 384, 770, 1645
Hsp92I	GRCGYC	1 cut(s)	817
HspAI	GCGC	2 cut(s)	602, 817
KasI	GGCGCC	1 cut(s)	816
Ksp22I	TGATCA	5 cut(s)	436, 477, 826, 996, 1716
KspI	CCGCGG	1 cut(s)	397
LmnI	GCTCC	1 cut(s)	1757
Lsp1109I	GCAGC	9 cut(s)	687, 1021, 1435, 1471, 1705, 1823, 1916, 1919, 1939
LweI	GCATC	9 cut(s)	110, 432, 520, 750, 1137, 1200, 1297, 1478, 1873
MaeI	CTAG	3 cut(s)	942, 1217, 1292
MaeII	ACGT	4 cut(s)	159, 384, 770, 1645
MaeIII	GTNAC	2 cut(s)	262, 571
MboII	GAAGA	9 cut(s)	56, 738, 760, 818, 1040, 1333, 1528, 1604, 1965
MflI	RGATCY	2 cut(s)	186, 1282
MhlI	GDGCHC	3 cut(s)	55, 660, 1576
MlsI	TGGCCA	2 cut(s)	311, 594
MluCI	AATT	7 cut(s)	271, 364, 895, 1404, 1607, 1628, 1969
MluNI	TGGCCA	2 cut(s)	311, 594
Mly113I	GGCGCC	1 cut(s)	817
MlyI	GAGTC	1 cut(s)	1612
Mox20I	TGGCCA	2 cut(s)	311, 594
Mph1103I	ATGCAT	2 cut(s)	1471, 1570
MroXI	GAANNNNTTC	1 cut(s)	326
MscI	TGGCCA	2 cut(s)	311, 594
MseI	TTAA	6 cut(s)	765, 1118, 1194, 1562, 1611, 1823
MslI	CAYNNNNRTG	2 cut(s)	1075, 1788
Msp20I	TGGCCA	2 cut(s)	311, 594
MspA1I	CMGCKG	5 cut(s)	396, 1002, 1312, 1814, 1910
MspI	CCGG	8 cut(s)	141, 285, 319, 378, 446, 576, 1600, 1887
MspR9I	CCNGG	9 cut(s)	141, 226, 286, 319, 361, 379, 577, 1106, 1444
Mva1269I	GAATGC	5 cut(s)	568, 633, 1059, 1087, 1453
MvaI	CCWGG	4 cut(s)	226, 361, 1106, 1444
MvnI	CGCG	2 cut(s)	396, 604
MwoI	GCNNNNNNNGC	5 cut(s)	230, 1490, 1693, 1808, 1817
NarI	GGCGCC	1 cut(s)	817
NciI	CCSGG	5 cut(s)	141, 286, 319, 379, 577
NdeI	CATATG	1 cut(s)	1014
NlaIV	GGNNCC	1 cut(s)	818
NmeAIII	GCCGAG	2 cut(s)	326, 793
NsiI	ATGCAT	2 cut(s)	1471, 1570
NspI	RCATGY	4 cut(s)	413, 1300, 1572, 1952
PaeI	GCATGC	2 cut(s)	1300, 1952
PagI	TCATGA	2 cut(s)	1129, 1317
PctI	GAATGC	5 cut(s)	568, 633, 1059, 1087, 1453
PdmI	GAANNNNTTC	1 cut(s)	326
PfeI	GAWTC	2 cut(s)	7, 1303
PfoI	TCCNGGA	2 cut(s)	359, 1442
PleI	GAGTC	1 cut(s)	1611
PluTI	GGCGCC	1 cut(s)	820
PpsI	GAGTC	1 cut(s)	1611
Ppu21I	YACGTR	1 cut(s)	1646
PsiI	TTATAA	1 cut(s)	1361
Psp124BI	GAGCTC	1 cut(s)	55
Psp6I	CCWGG	4 cut(s)	224, 359, 1104, 1442
PspEI	GGTNACC	1 cut(s)	571
PspGI	CCWGG	4 cut(s)	224, 359, 1104, 1442
PspN4I	GGNNCC	1 cut(s)	818
PspPI	GGNCC	2 cut(s)	316, 1850
PstI	CTGCAG	1 cut(s)	1489
PstNI	CAGNNNCTG	1 cut(s)	1910
PsuI	RGATCY	2 cut(s)	186, 1282
PvuII	CAGCTG	3 cut(s)	1312, 1814, 1910
RsaI	GTAC	4 cut(s)	383, 488, 1270, 1868
RsaNI	GTAC	4 cut(s)	382, 487, 1269, 1867
RseI	CAYNNNNRTG	2 cut(s)	1075, 1788
SacI	GAGCTC	1 cut(s)	55
SacII	CCGCGG	1 cut(s)	397
SalI	GTCGAC	1 cut(s)	1670
SaqAI	TTAA	6 cut(s)	765, 1118, 1194, 1562, 1611, 1823
Sau96I	GGNCC	2 cut(s)	316, 1850
ScaI	AGTACT	1 cut(s)	1270
SchI	GAGTC	1 cut(s)	1612
ScrFI	CCNGG	9 cut(s)	141, 226, 286, 319, 361, 379, 577, 1106, 1444
SduI	GDGCHC	3 cut(s)	55, 660, 1576
SfaNI	GCATC	9 cut(s)	110, 432, 520, 750, 1137, 1200, 1297, 1478, 1873
SfcI	CTRYAG	3 cut(s)	584, 1485, 1957
SfoI	GGCGCC	1 cut(s)	818
Sfr303I	CCGCGG	1 cut(s)	397
SgrBI	CCGCGG	1 cut(s)	397
SinI	GGWCC	1 cut(s)	1850
SmiI	ATTTAAAT	1 cut(s)	1612
SmiMI	CAYNNNNRTG	2 cut(s)	1075, 1788
SmlI	CTYRAG	1 cut(s)	1697
SmoI	CTYRAG	1 cut(s)	1697
SphI	GCATGC	2 cut(s)	1300, 1952
Sse9I	AATT	7 cut(s)	271, 364, 895, 1404, 1607, 1628, 1969
SsiI	CCGC	8 cut(s)	394, 396, 520, 539, 632, 1002, 1151, 1280
SspDI	GGCGCC	1 cut(s)	816
SspI	AATATT	1 cut(s)	931
SspMI	CTAG	3 cut(s)	942, 1217, 1292
SstI	GAGCTC	1 cut(s)	55
StyD4I	CCNGG	9 cut(s)	139, 224, 284, 317, 359, 377, 575, 1104, 1442
StyI	CCWWGG	1 cut(s)	312
SwaI	ATTTAAAT	1 cut(s)	1612
TaaI	ACNGT	4 cut(s)	458, 866, 1074, 1327
TaiI	ACGT	4 cut(s)	162, 387, 773, 1648
TaqI	TCGA	5 cut(s)	330, 528, 621, 1650, 1671
TasI	AATT	7 cut(s)	271, 364, 895, 1404, 1607, 1628, 1969
TatI	WGTACW	2 cut(s)	1268, 1866
TauI	GCSGC	6 cut(s)	396, 523, 541, 634, 1005, 1153
TfiI	GAWTC	2 cut(s)	7, 1303
Tru1I	TTAA	6 cut(s)	765, 1118, 1194, 1562, 1611, 1823
Tru9I	TTAA	6 cut(s)	765, 1118, 1194, 1562, 1611, 1823
TscAI	CASTG	4 cut(s)	180, 487, 871, 1077
TseI	GCWGC	9 cut(s)	700, 1034, 1423, 1484, 1693, 1811, 1904, 1907, 1952
TspGWI	ACGGA	2 cut(s)	268, 1842
TspRI	CASTG	4 cut(s)	180, 487, 871, 1077
VneI	GTGCAC	1 cut(s)	656
VpaK11BI	GGWCC	1 cut(s)	1850
XagI	CCTNNNNNAGG	1 cut(s)	1960
XapI	RAATTY	5 cut(s)	271, 364, 895, 1628, 1969
XceI	RCATGY	4 cut(s)	413, 1300, 1572, 1952
XmiI	GTMKAC	3 cut(s)	70, 162, 1671
XmnI	GAANNNNTTC	1 cut(s)	326
XspI	CTAG	3 cut(s)	942, 1217, 1292
ZrmI	AGTACT	1 cut(s)	1270
Zsp2I	ATGCAT	2 cut(s)	1471, 1570

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.