MD10G1174300.v1.1

Tetratricopeptide repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0003427
Physical Location & Seq
Reverse (-)
26673714 .. 26674932
1219 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1174300.v1.1.491

Sequence Viewer

Length: 726 bp
ATGGCGCTGCTGATGGAAAAGGGGTCCCAACCCCAAACCGAAAGCGAAAAGGCCGACCTTGACGCCATCGCCGCCCTCAAAGAAAGCGCTGCCATTGAACTCAAGGAAAAGGGAAACGAGTGTGTGAAAAAGGGTAAAAAGCATTTCGCTGAAGCCATAGATTGCTACACGAGGGCAATCAATCAGCAAGCTTTGAGCGATTCCGACACCTCAATTCTCTTTTCGAATCGAGCCCATGTCAATCTTTTGCTCGGTAACTACAGGCGCGCTCTTACTGATGCCGAGGAAGCAATCAAGCTCTGCCCCACTAATCTCAAGGCTTTGTACCGTGCTGCGAAAGCGTCTTTTTCGTTGAATTTGTTGTCTGAATCGACATCACATTGCGAAAACGGGCTTAAACATGACCCGGCTAACGAGGAGCTGAGGAAGCTGTTGAGGCAGATTGAGTCGAAGAAAATGGAACATGAGCAGCGTGAGGCTCAAGTCTCCAAGGCTATCGCAGAGGCTAAGGACCTTGTTTCCGCGATTGAAGGTCGAGGCATGAAGCTTGGGAAGGCAATGTATCGAGAACTTACCGGATTACGAAAGCCTGTACTAGACAAGAATAACATGCTTCATTGGCCAGTTCTTCTGCTGTATGCAGAGGTTATGTCCAGTGACTTCATTGAGGATTTCTGTGAGACCGACATGTTGTCAGCTCATCTCGACATGATATCCTTCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPR_19 TPR_8

Protein Analysis

242

Amino Acids

27.0

Weight (kDa)

6.02

Isoelectric Point (pI)

34.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_BSK1_C PF25575 31 - 109 4.1e-06 Serine/threonine-protein kinase BSK1-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 267, 524
AciI CCGC 2 cut(s) 72, 522
AcoI YGGCCR 1 cut(s) 620
AcsI RAATTY 1 cut(s) 355
AcuI CTGAAG 1 cut(s) 171
AcyI GRCGYC 1 cut(s) 63
AfaI GTAC 2 cut(s) 326, 594
AfeI AGCGCT 1 cut(s) 88
AflIII ACRYGT 1 cut(s) 687
AgsI TTSAA 3 cut(s) 98, 355, 530
AhdI GACNNNNNGTC 1 cut(s) 691
AluBI AGCT 6 cut(s) 191, 298, 421, 430, 547, 698
AluI AGCT 6 cut(s) 191, 298, 421, 430, 547, 698
Alw26I GTCTC 2 cut(s) 490, 674
Aor51HI AGCGCT 1 cut(s) 88
AoxI GGCC 2 cut(s) 51, 620
ApeKI GCWGC 4 cut(s) 7, 89, 332, 469
ApoI RAATTY 1 cut(s) 355
AspLEI GCGC 4 cut(s) 7, 89, 267, 269
AspS9I GGNCC 2 cut(s) 24, 511
AsuC2I CCSGG 1 cut(s) 407
AsuII TTCGAA 1 cut(s) 224
AvaII GGWCC 2 cut(s) 24, 511
BalI TGGCCA 1 cut(s) 622
BanII GRGCYC 1 cut(s) 235
BauI CACGAG 1 cut(s) 169
BbvCI CCTCAGC 1 cut(s) 422
BbvI GCAGC 3 cut(s) 76, 319, 481
BccI CCATC 2 cut(s) 7, 74
BcnI CCSGG 1 cut(s) 407
BcoDI GTCTC 2 cut(s) 490, 674
BfaI CTAG 1 cut(s) 596
BfmI CTRYAG 1 cut(s) 259
BfoI RGCGCY 2 cut(s) 8, 90
BisI GCNGC 5 cut(s) 8, 72, 90, 333, 470
BlsI GCNGC 5 cut(s) 9, 73, 91, 334, 471
Bme1390I CCNGG 1 cut(s) 407
Bme18I GGWCC 2 cut(s) 24, 511
BmeRI GACNNNNNGTC 1 cut(s) 691
BmgT120I GGNCC 2 cut(s) 24, 511
BmiI GGNNCC 2 cut(s) 25, 26
BmrFI CCNGG 1 cut(s) 407
BmsI GCATC 1 cut(s) 268
Bpu10I CCTNAGC 2 cut(s) 422, 507
Bpu14I TTCGAA 1 cut(s) 224
BpuEI CTTGAG 3 cut(s) 86, 299, 465
BpuMI CCSGG 1 cut(s) 407
BsaHI GRCGYC 1 cut(s) 63
BsaI GGTCTC 1 cut(s) 674
BsaJI CCNNGG 2 cut(s) 282, 489
BsaWI WCCGGW 1 cut(s) 575
Bse1I ACTGG 2 cut(s) 623, 654
Bse3DI GCAATG 2 cut(s) 379, 564
BseDI CCNNGG 2 cut(s) 282, 489
BseMI GCAATG 2 cut(s) 379, 564
BseMII CTCAG 1 cut(s) 413
BseNI ACTGG 2 cut(s) 623, 654
BsePI GCGCGC 1 cut(s) 265
BseRI GAGGAG 1 cut(s) 431
BseXI GCAGC 3 cut(s) 76, 319, 481
Bsh1236I CGCG 2 cut(s) 267, 524
BshFI GGCC 2 cut(s) 53, 622
BsiSI CCGG 2 cut(s) 407, 576
BslFI GGGAC 1 cut(s) 10
BsmAI GTCTC 2 cut(s) 490, 674
BsmFI GGGAC 1 cut(s) 10
BsnI GGCC 2 cut(s) 53, 622
Bso31I GGTCTC 1 cut(s) 674
Bsp119I TTCGAA 1 cut(s) 224
Bsp1286I GDGCHC 1 cut(s) 235
BspACI CCGC 2 cut(s) 72, 522
BspANI GGCC 2 cut(s) 53, 622
BspCNI CTCAG 1 cut(s) 414
BspFNI CGCG 2 cut(s) 267, 524
BspLI GGNNCC 2 cut(s) 25, 26
BspT104I TTCGAA 1 cut(s) 224
BspTNI GGTCTC 1 cut(s) 674
BsrDI GCAATG 2 cut(s) 379, 564
BsrI ACTGG 2 cut(s) 623, 654
BssECI CCNNGG 2 cut(s) 282, 489
BssHII GCGCGC 1 cut(s) 265
BssNI GRCGYC 1 cut(s) 63
BssSI CACGAG 1 cut(s) 169
BssT1I CCWWGG 1 cut(s) 489
Bst2BI CACGAG 1 cut(s) 169
Bst4CI ACNGT 1 cut(s) 329
BstACI GRCGYC 1 cut(s) 63
BstBI TTCGAA 1 cut(s) 224
BstC8I GCNNGC 2 cut(s) 189, 267
BstDEI CTNAG 2 cut(s) 422, 507
BstFNI CGCG 2 cut(s) 267, 524
BstH2I RGCGCY 2 cut(s) 8, 90
BstHHI GCGC 4 cut(s) 7, 89, 267, 269
BstMAI GTCTC 2 cut(s) 490, 674
BstMWI GCNNNNNNNGC 6 cut(s) 71, 287, 338, 427, 436, 619
BstNSI RCATGY 2 cut(s) 613, 691
BstSCI CCNGG 1 cut(s) 405
BstSFI CTRYAG 1 cut(s) 259
BstUI CGCG 2 cut(s) 267, 524
BstV1I GCAGC 3 cut(s) 76, 319, 481
BsuRI GGCC 2 cut(s) 53, 622
BtgZI GCGATG 1 cut(s) 52
BtsIMutI CAGTG 1 cut(s) 661
Cac8I GCNNGC 2 cut(s) 189, 267
CfoI GCGC 4 cut(s) 7, 89, 267, 269
Cfr13I GGNCC 2 cut(s) 24, 511
CseI GACGC 2 cut(s) 71, 330
Csp6I GTAC 2 cut(s) 325, 593
CviAII CATG 7 cut(s) 236, 401, 464, 541, 610, 688, 709
CviQI GTAC 2 cut(s) 325, 593
DdeI CTNAG 2 cut(s) 422, 507
DriI GACNNNNNGTC 1 cut(s) 691
EaeI YGGCCR 1 cut(s) 620
Eam1105I GACNNNNNGTC 1 cut(s) 691
Eco130I CCWWGG 1 cut(s) 489
Eco24I GRGCYC 1 cut(s) 235
Eco31I GGTCTC 1 cut(s) 674
Eco32I GATATC 1 cut(s) 714
Eco47I GGWCC 2 cut(s) 24, 511
Eco47III AGCGCT 1 cut(s) 88
Eco57I CTGAAG 1 cut(s) 171
EcoO109I RGGNCCY 2 cut(s) 24, 511
EcoRV GATATC 1 cut(s) 714
EcoT14I CCWWGG 1 cut(s) 489
EcoT38I GRGCYC 1 cut(s) 235
ErhI CCWWGG 1 cut(s) 489
FaeI CATG 7 cut(s) 239, 404, 467, 544, 613, 691, 712
FaqI GGGAC 1 cut(s) 10
FatI CATG 7 cut(s) 235, 400, 463, 540, 609, 687, 708
Fnu4HI GCNGC 5 cut(s) 8, 72, 90, 333, 470
FriOI GRGCYC 1 cut(s) 235
Fsp4HI GCNGC 5 cut(s) 8, 72, 90, 333, 470
FspBI CTAG 1 cut(s) 596
GlaI GCGC 4 cut(s) 6, 88, 266, 268
GluI GCNGC 5 cut(s) 8, 72, 90, 333, 470
HaeII RGCGCY 2 cut(s) 8, 90
HaeIII GGCC 2 cut(s) 53, 622
HapII CCGG 2 cut(s) 407, 576
HgaI GACGC 2 cut(s) 71, 330
HhaI GCGC 4 cut(s) 7, 89, 267, 269
Hin1I GRCGYC 1 cut(s) 63
Hin1II CATG 7 cut(s) 239, 404, 467, 544, 613, 691, 712
Hin6I GCGC 4 cut(s) 5, 87, 265, 267
HinP1I GCGC 4 cut(s) 5, 87, 265, 267
HindIII AAGCTT 2 cut(s) 189, 545
HinfI GANTC 4 cut(s) 200, 226, 368, 446
HpaII CCGG 2 cut(s) 407, 576
Hpy188I TCNGA 2 cut(s) 205, 367
Hpy188III TCNNGA 2 cut(s) 566, 704
HpyAV CCTTC 2 cut(s) 524, 547
HpyCH4III ACNGT 1 cut(s) 329
HpyCH4V TGCA 1 cut(s) 641
HpyF10VI GCNNNNNNNGC 6 cut(s) 71, 287, 338, 427, 436, 619
HpyF3I CTNAG 2 cut(s) 422, 507
Hsp92I GRCGYC 1 cut(s) 63
Hsp92II CATG 7 cut(s) 239, 404, 467, 544, 613, 691, 712
HspAI GCGC 4 cut(s) 5, 87, 265, 267
KflI GGGWCCC 1 cut(s) 24
LmnI GCTCC 1 cut(s) 418
LpnPI CCDG 6 cut(s) 247, 420, 589, 603, 636, 667
Lsp1109I GCAGC 3 cut(s) 76, 319, 481
LweI GCATC 1 cut(s) 268
MaeI CTAG 1 cut(s) 596
MaeIII GTNAC 2 cut(s) 254, 656
MboII GAAGA 2 cut(s) 463, 620
MhlI GDGCHC 1 cut(s) 235
MlsI TGGCCA 1 cut(s) 622
MluCI AATT 2 cut(s) 213, 355
MluNI TGGCCA 1 cut(s) 622
MlyI GAGTC 1 cut(s) 455
MmeI TCCRAC 1 cut(s) 228
Mox20I TGGCCA 1 cut(s) 622
MscI TGGCCA 1 cut(s) 622
MseI TTAA 2 cut(s) 396, 724
Msp20I TGGCCA 1 cut(s) 622
MspI CCGG 2 cut(s) 407, 576
MspR9I CCNGG 1 cut(s) 407
MvnI CGCG 2 cut(s) 267, 524
MwoI GCNNNNNNNGC 6 cut(s) 71, 287, 338, 427, 436, 619
NciI CCSGG 1 cut(s) 407
NlaIII CATG 7 cut(s) 239, 404, 467, 544, 613, 691, 712
NlaIV GGNNCC 2 cut(s) 25, 26
NmeAIII GCCGAG 1 cut(s) 307
NmuCI GTSAC 1 cut(s) 656
NspI RCATGY 2 cut(s) 613, 691
NspV TTCGAA 1 cut(s) 224
PauI GCGCGC 1 cut(s) 265
PciI ACATGT 1 cut(s) 687
PfeI GAWTC 3 cut(s) 200, 226, 368
PkrI GCNGC 5 cut(s) 9, 73, 91, 334, 471
PleI GAGTC 1 cut(s) 454
PpsI GAGTC 1 cut(s) 454
PpuMI RGGWCCY 2 cut(s) 24, 511
PscI ACATGT 1 cut(s) 687
Psp5II RGGWCCY 2 cut(s) 24, 511
PspN4I GGNNCC 2 cut(s) 25, 26
PspPI GGNCC 2 cut(s) 24, 511
PspPPI RGGWCCY 2 cut(s) 24, 511
PteI GCGCGC 1 cut(s) 265
RsaI GTAC 2 cut(s) 326, 594
RsaNI GTAC 2 cut(s) 325, 593
SaqAI TTAA 2 cut(s) 396, 724
SatI GCNGC 5 cut(s) 8, 72, 90, 333, 470
Sau96I GGNCC 2 cut(s) 24, 511
SchI GAGTC 1 cut(s) 455
ScrFI CCNGG 1 cut(s) 407
SduI GDGCHC 1 cut(s) 235
SfaNI GCATC 1 cut(s) 268
SfcI CTRYAG 1 cut(s) 259
SfuI TTCGAA 1 cut(s) 224
SinI GGWCC 2 cut(s) 24, 511
SmlI CTYRAG 3 cut(s) 101, 314, 480
SmoI CTYRAG 3 cut(s) 101, 314, 480
Sse9I AATT 2 cut(s) 213, 355
SsiI CCGC 2 cut(s) 72, 522
SspMI CTAG 1 cut(s) 596
StyD4I CCNGG 1 cut(s) 405
StyI CCWWGG 1 cut(s) 489
TaaI ACNGT 1 cut(s) 329
TaqI TCGA 7 cut(s) 224, 229, 371, 449, 535, 565, 705
TaqII GACCGA 1 cut(s) 698
TasI AATT 2 cut(s) 213, 355
TatI WGTACW 1 cut(s) 592
TauI GCSGC 1 cut(s) 74
TfiI GAWTC 3 cut(s) 200, 226, 368
Tru1I TTAA 2 cut(s) 396, 724
Tru9I TTAA 2 cut(s) 396, 724
TscAI CASTG 1 cut(s) 661
TseFI GTSAC 1 cut(s) 656
TseI GCWGC 4 cut(s) 7, 89, 332, 469
Tsp45I GTSAC 1 cut(s) 656
TspDTI ATGAA 3 cut(s) 557, 605, 652
TspRI CASTG 1 cut(s) 661
VpaK11BI GGWCC 2 cut(s) 24, 511
XapI RAATTY 1 cut(s) 355
XceI RCATGY 2 cut(s) 613, 691
XspI CTAG 1 cut(s) 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.