MD10G1264700.v1.1

Tobamovirus multiplication protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0001716
Physical Location & Seq
Forward (+)
35831556 .. 35837493
5938 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1264700.v1.1.491

Sequence Viewer

Length: 1074 bp
ATGTTTGGATTGAGAGAAGGGTCTTGCTTTCCGAGGGTGTTGGTGGGAGTGAATGTGGGTCTTGCTCTGGTTGATGGCATTATAGCATTTCTTGCATTTTATCAGTTAATTAGAATTCACTTGAGGAATTCACAACTGGGTTGGACTCGGCAAAAAGTTTTTCATCTGTTGATTGGCTCTTCTAATTTGGGTTGCTTAATTTATTTTGTATTAACTCTTTTTGCTGCTTGCAAGGGGTGGCAATGTTGGTCTAACTCTTGTGGTTTTAGCCTCATGGCATTGCCCGAAATTCTGTTTTTTGCTGCATTTCTTCTACTTTTATCTTTCTGGGTTGACCTGTGCCATCAGGCAGATGATGAGGATGAGGAGGATGAAGGGAGTTTTCGGGAAGCTTTGTTGGAGAAGACTTTTAGCAAACCGAATTCATTGGACCGAGATAGTTATCGAAACTGTTTTCCTCTGCGATTTGTTCATATTGGAAGCCGCCAGAGAATTGTAATTATGGTGACCGTGCTAGTTTTCGTTATAATGATAGCAAGTGCAGTGATAATTTGGATCGGGATGGGAAAAAATTCTATTGATTCTGCACTGGTGGCTCGGGTATATGTGGATCTCTTTGCAATAGCTATACTAGTATTGGGAGGAGCATTAGCGTGTTATGGGCTCCTATTATGCCTAAGGATGAGAAATGTTAGATCAGAGAGAGCTTCTTCTGAGATGTGGAAGGTTGCATGTCTAGCTGTTGTTTCCATATTATGTTTCACCTCAAGTTCTTTCGTGGCTCTGTTAACTGATATTCCTATGTTTTACGATTGGCATCAACTGCATATGAATGATGTTTATACTTCTCTTTTACTGATTTTATATTACTTTGTAGGTTCATCAATACCCTCAGCCTTGGTCTTATGGGTCGTGAGAGAATTACCACCCTCAATAACAGCTAACATACGAGAAGAACCATCAACAATAACCTTTGTCAGTGGCGGTTCCACAACATTACAACATCCTCAAAGCTGGACAGCTGCAATGAGCTTGCGGAACCAGGTGCAGATATCAAGAGCAAGTCCCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1084

Protein Analysis

358

Amino Acids

39.96

Weight (kDa)

7.02

Isoelectric Point (pI)

48.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
THH1_TOM1-3_dom PF06454 17 - 118 8.3e-15 THH1/TOM1/TOM3 domain
THH1_TOM1-3_dom PF06454 167 - 312 1.9e-09 THH1/TOM1/TOM3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 527
AciI CCGC 3 cut(s) 484, 984, 1036
AclWI GGATC 2 cut(s) 563, 618
AcsI RAATTY 5 cut(s) 114, 127, 288, 421, 571
AhlI ACTAGT 1 cut(s) 631
AjnI CCWGG 1 cut(s) 1041
AluBI AGCT 8 cut(s) 392, 626, 707, 740, 941, 1014, 1022, 1032
AluI AGCT 8 cut(s) 392, 626, 707, 740, 941, 1014, 1022, 1032
AlwI GGATC 2 cut(s) 563, 618
Ama87I CYCGRG 1 cut(s) 597
ApeKI GCWGC 3 cut(s) 224, 302, 1022
ApoI RAATTY 5 cut(s) 114, 127, 288, 421, 571
Asp700I GAANNNNTTC 1 cut(s) 571
AspS9I GGNCC 1 cut(s) 430
AsuHPI GGTGA 2 cut(s) 517, 754
AvaI CYCGRG 1 cut(s) 597
AvaII GGWCC 1 cut(s) 430
AxyI CCTNAGG 1 cut(s) 677
BanII GRGCYC 1 cut(s) 666
BbsI GAAGAC 1 cut(s) 410
BbvCI CCTCAGC 1 cut(s) 892
BbvI GCAGC 3 cut(s) 211, 289, 1009
BccI CCATC 4 cut(s) 68, 351, 556, 967
BciT130I CCWGG 1 cut(s) 1043
BcuI ACTAGT 1 cut(s) 631
BfaI CTAG 3 cut(s) 515, 632, 737
BisI GCNGC 4 cut(s) 225, 303, 484, 1023
BlsI GCNGC 4 cut(s) 226, 304, 485, 1024
Bme1390I CCNGG 1 cut(s) 1043
Bme18I GGWCC 1 cut(s) 430
BmeT110I CYCGRG 1 cut(s) 597
BmgT120I GGNCC 1 cut(s) 430
BmiI GGNNCC 3 cut(s) 665, 988, 1040
BmrFI CCNGG 1 cut(s) 1043
BmrI ACTGGG 1 cut(s) 146
BmsI GCATC 1 cut(s) 826
BmuI ACTGGG 1 cut(s) 146
BpiI GAAGAC 1 cut(s) 410
Bpu10I CCTNAGC 1 cut(s) 892
BpuEI CTTGAG 2 cut(s) 142, 751
BsaBI GATNNNNATC 2 cut(s) 441, 816
BsaJI CCNNGG 2 cut(s) 32, 897
BsaXI ACNNNNNCTCC 2 cut(s) 39, 69
Bse1I ACTGG 2 cut(s) 141, 594
Bse21I CCTNAGG 1 cut(s) 677
Bse3DI GCAATG 3 cut(s) 248, 278, 1032
Bse8I GATNNNNATC 2 cut(s) 441, 816
BseBI CCWGG 1 cut(s) 1043
BseDI CCNNGG 2 cut(s) 32, 897
BseGI GGATG 5 cut(s) 367, 376, 567, 687, 1003
BseJI GATNNNNATC 2 cut(s) 441, 816
BseMI GCAATG 3 cut(s) 248, 278, 1032
BseMII CTCAG 2 cut(s) 705, 906
BseNI ACTGG 2 cut(s) 141, 594
BseRI GAGGAG 2 cut(s) 380, 657
BseXI GCAGC 3 cut(s) 211, 289, 1009
BsgI GTGCAG 3 cut(s) 561, 570, 1067
BsiHKCI CYCGRG 1 cut(s) 597
BslFI GGGAC 1 cut(s) 1050
BsmFI GGGAC 1 cut(s) 1050
BsoBI CYCGRG 1 cut(s) 597
Bsp1286I GDGCHC 1 cut(s) 666
Bsp143I GATC 3 cut(s) 555, 610, 695
BspACI CCGC 3 cut(s) 484, 984, 1036
BspCNI CTCAG 2 cut(s) 706, 905
BspLI GGNNCC 3 cut(s) 665, 988, 1040
BspPI GGATC 2 cut(s) 563, 618
BspQI GCTCTTC 1 cut(s) 184
BsrDI GCAATG 3 cut(s) 248, 278, 1032
BsrI ACTGG 2 cut(s) 141, 594
BssECI CCNNGG 2 cut(s) 32, 897
BssMI GATC 3 cut(s) 555, 610, 695
BssT1I CCWWGG 1 cut(s) 897
Bst2UI CCWGG 1 cut(s) 1043
Bst4CI ACNGT 2 cut(s) 452, 511
Bst6I CTCTTC 1 cut(s) 184
BstAPI GCANNNNNTGC 2 cut(s) 92, 823
BstC8I GCNNGC 2 cut(s) 229, 1034
BstDEI CTNAG 3 cut(s) 677, 714, 892
BstEII GGTNACC 1 cut(s) 505
BstF5I GGATG 5 cut(s) 367, 376, 567, 687, 1003
BstKTI GATC 3 cut(s) 558, 613, 698
BstMBI GATC 3 cut(s) 555, 610, 695
BstMWI GCNNNNNNNGC 4 cut(s) 92, 593, 737, 823
BstNI CCWGG 1 cut(s) 1043
BstNSI RCATGY 1 cut(s) 735
BstPI GGTNACC 1 cut(s) 505
BstSCI CCNGG 1 cut(s) 1041
BstV1I GCAGC 3 cut(s) 211, 289, 1009
BstV2I GAAGAC 1 cut(s) 410
BstX2I RGATCY 1 cut(s) 610
BstYI RGATCY 1 cut(s) 610
Bsu36I CCTNAGG 1 cut(s) 677
BtsCI GGATG 5 cut(s) 367, 376, 567, 687, 1003
BtsI GCAGTG 1 cut(s) 549
BtsIMutI CAGTG 3 cut(s) 549, 587, 985
Cac8I GCNNGC 2 cut(s) 229, 1034
Cfr13I GGNCC 1 cut(s) 430
CsiI ACCWGGT 1 cut(s) 1041
CviAII CATG 2 cut(s) 274, 732
DdeI CTNAG 3 cut(s) 677, 714, 892
DpnI GATC 3 cut(s) 557, 612, 697
DpnII GATC 3 cut(s) 555, 610, 695
Eam1104I CTCTTC 1 cut(s) 184
EarI CTCTTC 1 cut(s) 184
Eco130I CCWWGG 1 cut(s) 897
Eco24I GRGCYC 1 cut(s) 666
Eco32I GATATC 1 cut(s) 1053
Eco47I GGWCC 1 cut(s) 430
Eco81I CCTNAGG 1 cut(s) 677
Eco88I CYCGRG 1 cut(s) 597
Eco91I GGTNACC 1 cut(s) 505
EcoO65I GGTNACC 1 cut(s) 505
EcoRI GAATTC 3 cut(s) 114, 127, 421
EcoRII CCWGG 1 cut(s) 1041
EcoRV GATATC 1 cut(s) 1053
EcoT14I CCWWGG 1 cut(s) 897
EcoT38I GRGCYC 1 cut(s) 666
ErhI CCWWGG 1 cut(s) 897
FaeI CATG 2 cut(s) 277, 735
FaqI GGGAC 1 cut(s) 1050
FatI CATG 2 cut(s) 273, 731
FauNDI CATATG 1 cut(s) 828
Fnu4HI GCNGC 4 cut(s) 225, 303, 484, 1023
FokI GGATG 5 cut(s) 374, 383, 574, 694, 990
FriOI GRGCYC 1 cut(s) 666
Fsp4HI GCNGC 4 cut(s) 225, 303, 484, 1023
FspBI CTAG 3 cut(s) 515, 632, 737
GluI GCNGC 4 cut(s) 225, 303, 484, 1023
Hin1II CATG 2 cut(s) 277, 735
HincII GTYRAC 2 cut(s) 334, 789
HindII GTYRAC 2 cut(s) 334, 789
HindIII AAGCTT 1 cut(s) 390
HinfI GANTC 2 cut(s) 145, 581
HpaI GTTAAC 1 cut(s) 789
HphI GGTGA 2 cut(s) 517, 754
Hpy166II GTNNAC 2 cut(s) 334, 789
Hpy188I TCNGA 3 cut(s) 33, 700, 715
Hpy188III TCNNGA 4 cut(s) 386, 559, 913, 1056
Hpy8I GTNNAC 2 cut(s) 334, 789
HpyAV CCTTC 3 cut(s) 11, 368, 718
HpyCH4III ACNGT 2 cut(s) 452, 511
HpyF10VI GCNNNNNNNGC 4 cut(s) 92, 593, 737, 823
HpyF3I CTNAG 3 cut(s) 677, 714, 892
Hsp92II CATG 2 cut(s) 277, 735
KspAI GTTAAC 1 cut(s) 789
Kzo9I GATC 3 cut(s) 555, 610, 695
LguI GCTCTTC 1 cut(s) 184
LmnI GCTCC 2 cut(s) 644, 669
Lsp1109I GCAGC 3 cut(s) 211, 289, 1009
LweI GCATC 1 cut(s) 826
MabI ACCWGGT 1 cut(s) 1041
MaeI CTAG 3 cut(s) 515, 632, 737
MaeIII GTNAC 1 cut(s) 505
MalI GATC 3 cut(s) 557, 612, 697
MboI GATC 3 cut(s) 555, 610, 695
MboII GAAGA 5 cut(s) 171, 302, 415, 702, 965
MflI RGATCY 1 cut(s) 610
MhlI GDGCHC 1 cut(s) 666
MlyI GAGTC 1 cut(s) 139
MmeI TCCRAC 2 cut(s) 122, 378
MroXI GAANNNNTTC 1 cut(s) 571
MseI TTAA 4 cut(s) 107, 197, 212, 788
MslI CAYNNNNRTG 2 cut(s) 652, 831
MspA1I CMGCKG 1 cut(s) 1022
MspR9I CCNGG 1 cut(s) 1043
MvaI CCWGG 1 cut(s) 1043
MwoI GCNNNNNNNGC 4 cut(s) 92, 593, 737, 823
NdeI CATATG 1 cut(s) 828
NdeII GATC 3 cut(s) 555, 610, 695
NlaIII CATG 2 cut(s) 277, 735
NlaIV GGNNCC 3 cut(s) 665, 988, 1040
NmeAIII GCCGAG 1 cut(s) 127
NmuCI GTSAC 1 cut(s) 505
NspI RCATGY 1 cut(s) 735
PciSI GCTCTTC 1 cut(s) 184
PdmI GAANNNNTTC 1 cut(s) 571
PfeI GAWTC 1 cut(s) 581
PkrI GCNGC 4 cut(s) 226, 304, 485, 1024
PleI GAGTC 1 cut(s) 139
PpsI GAGTC 1 cut(s) 139
PsiI TTATAA 1 cut(s) 527
Psp6I CCWGG 1 cut(s) 1041
PspEI GGTNACC 1 cut(s) 505
PspGI CCWGG 1 cut(s) 1041
PspN4I GGNNCC 3 cut(s) 665, 988, 1040
PspPI GGNCC 1 cut(s) 430
PsuI RGATCY 1 cut(s) 610
PvuII CAGCTG 1 cut(s) 1022
RseI CAYNNNNRTG 2 cut(s) 652, 831
SapI GCTCTTC 1 cut(s) 184
SaqAI TTAA 4 cut(s) 107, 197, 212, 788
SatI GCNGC 4 cut(s) 225, 303, 484, 1023
Sau3AI GATC 3 cut(s) 555, 610, 695
Sau96I GGNCC 1 cut(s) 430
SchI GAGTC 1 cut(s) 139
ScrFI CCNGG 1 cut(s) 1043
SduI GDGCHC 1 cut(s) 666
SexAI ACCWGGT 1 cut(s) 1041
SfaNI GCATC 1 cut(s) 826
SinI GGWCC 1 cut(s) 430
SmiMI CAYNNNNRTG 2 cut(s) 652, 831
SmlI CTYRAG 2 cut(s) 121, 766
SmoI CTYRAG 2 cut(s) 121, 766
SpeI ACTAGT 1 cut(s) 631
SsiI CCGC 3 cut(s) 484, 984, 1036
SspMI CTAG 3 cut(s) 515, 632, 737
StyD4I CCNGG 1 cut(s) 1041
StyI CCWWGG 1 cut(s) 897
TaaI ACNGT 2 cut(s) 452, 511
TaqI TCGA 1 cut(s) 445
TaqII GACCGA 1 cut(s) 447
TauI GCSGC 1 cut(s) 486
TfiI GAWTC 1 cut(s) 581
Tru1I TTAA 4 cut(s) 107, 197, 212, 788
Tru9I TTAA 4 cut(s) 107, 197, 212, 788
TscAI CASTG 3 cut(s) 549, 594, 985
TseFI GTSAC 1 cut(s) 505
TseI GCWGC 3 cut(s) 224, 302, 1022
Tsp45I GTSAC 1 cut(s) 505
TspDTI ATGAA 6 cut(s) 152, 387, 414, 461, 845, 870
TspRI CASTG 3 cut(s) 549, 594, 985
VpaK11BI GGWCC 1 cut(s) 430
XapI RAATTY 5 cut(s) 114, 127, 288, 421, 571
XceI RCATGY 1 cut(s) 735
XmnI GAANNNNTTC 1 cut(s) 571
XspI CTAG 3 cut(s) 515, 632, 737
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.