Rw2G040140

Tobamovirus multiplication protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001716
Physical Location & Seq
Reverse (-)
65607023 .. 65612211
5189 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G040140.1

Sequence Viewer

Length: 966 bp
ATGAAATTAAGATCCCAGAAAAGATTGTGTGGTCATATTATGTTCCTTATCGAGTTAAACAATCCAATTTCTATTTTGGCAGGTTACTTCCTATATTTTACATCTACACTGATTGCCACTTTTAAGCAGTGGTTTTGCTGGTCTAATGTCTGCGGGTTCATTCTAATGGCCTACCCTAAAATTCTGTTGCTTGCAGCTTTTCTTCTAGTCCTATCCTTTTGGGTGGACCTTTGCCATCAGGCAAATGATGAAGAGGATGATGATGATGATGACAGTAGCATGCAGCATGCCTTGTTGGAAAATTCGAAGAATAAACACCGTTCATCAGTCACAAATGGTCATAGGATTTGTTGCTCATTCCCAAGCATTCATGTTGGAAGTCACCAGAAATTTGTGATTGCGGTTGTTGGGTTTGTCCTTATTTTGATGATGTCATTTTCTGTGGTAATCTGGATTGGTGCGGGGAAGAATCCCATTGATTCTTCAGCAGTGGCTGAGGTATATGAAGACCTTCTCGCCTTTGTAGTTCTTTTTTTAGGAGGAGGATTAGGTTGCTATGGCATTCTGCTACTTAGTAAGTTAAGGAAAGTACGGTCTGAGAAAGCTTCATCTGAAATGTGGAAGGTTGGCGGTTTAGCAGTTGCTTCTGTTGTATGTTTTACATCATGTGCATTAGTAGCTCTTCTTACAGATATTCCGCTATATGAACCTTGGAGGCTGAAAAAAGCATATGGTGGGAAAGCGCTAGTTTTTCTGATGCTGTACTATTTTATAGGCTCTTGGGTACCCTCAGCTTTTGTATTATGGTTCATGAGAGAATTACCGCCACCAATCACCAATCGCCAACGGGAACAATCAAGAACAATTACTTTTATCAGCTATGCTGGAGTGGGCCGACAGCATCCTCGTCACTGGGCTACAGTAACAAGTTCAAATAATCAGGCATCGCGAGCAAGTCCCATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1084

Protein Analysis

321

Amino Acids

36.01

Weight (kDa)

8.92

Isoelectric Point (pI)

41.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
THH1_TOM1-3_dom PF06454 142 - 283 1.4e-06 THH1/TOM1/TOM3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 71
Acc65I GGTACC 1 cut(s) 784
AccB1I GGYRCC 1 cut(s) 784
AccII CGCG 1 cut(s) 949
AciI CCGC 6 cut(s) 153, 401, 461, 630, 698, 824
AclWI GGATC 1 cut(s) 6
AcsI RAATTY 3 cut(s) 180, 301, 389
AcuI CTGAAG 1 cut(s) 468
AfaI GTAC 3 cut(s) 591, 764, 786
AfeI AGCGCT 1 cut(s) 744
AgsI TTSAA 1 cut(s) 933
AluBI AGCT 5 cut(s) 197, 605, 680, 794, 879
AluI AGCT 5 cut(s) 197, 605, 680, 794, 879
AlwI GGATC 1 cut(s) 6
AlwNI CAGNNNCTG 1 cut(s) 494
Aor51HI AGCGCT 1 cut(s) 744
AoxI GGCC 2 cut(s) 168, 892
ApeKI GCWGC 2 cut(s) 194, 283
ApoI RAATTY 3 cut(s) 180, 301, 389
Asp700I GAANNNNTTC 1 cut(s) 510
Asp718I GGTACC 1 cut(s) 784
AspLEI GCGC 1 cut(s) 745
AspS9I GGNCC 2 cut(s) 226, 892
AsuHPI GGTGA 2 cut(s) 374, 826
AsuII TTCGAA 1 cut(s) 305
AvaII GGWCC 1 cut(s) 226
BanI GGYRCC 1 cut(s) 784
BbsI GAAGAC 1 cut(s) 513
BbvCI CCTCAGC 2 cut(s) 495, 790
BbvI GCAGC 2 cut(s) 206, 295
BccI CCATC 1 cut(s) 243
BfaI CTAG 2 cut(s) 206, 746
BfmI CTRYAG 1 cut(s) 918
BfoI RGCGCY 1 cut(s) 746
BfuAI ACCTGC 1 cut(s) 71
BisI GCNGC 2 cut(s) 195, 284
BlsI GCNGC 2 cut(s) 196, 285
Bme18I GGWCC 1 cut(s) 226
BmgT120I GGNCC 2 cut(s) 226, 892
BmiI GGNNCC 1 cut(s) 786
BmrI ACTGGG 1 cut(s) 922
BmsI GCATC 3 cut(s) 747, 910, 953
BmuI ACTGGG 1 cut(s) 922
BpiI GAAGAC 1 cut(s) 513
BpmI CTGGAG 1 cut(s) 906
Bpu10I CCTNAGC 2 cut(s) 495, 790
Bpu14I TTCGAA 1 cut(s) 305
BsaJI CCNNGG 1 cut(s) 710
Bse1I ACTGG 1 cut(s) 917
BseDI CCNNGG 1 cut(s) 710
BseGI GGATG 2 cut(s) 262, 901
BseMII CTCAG 3 cut(s) 486, 588, 804
BseNI ACTGG 1 cut(s) 917
BseRI GAGGAG 1 cut(s) 555
BseXI GCAGC 2 cut(s) 206, 295
Bsh1236I CGCG 1 cut(s) 949
BshFI GGCC 2 cut(s) 170, 894
BshNI GGYRCC 1 cut(s) 784
BslFI GGGAC 1 cut(s) 942
BsmFI GGGAC 1 cut(s) 942
BsmI GAATGC 2 cut(s) 366, 561
BsnI GGCC 2 cut(s) 170, 894
Bsp119I TTCGAA 1 cut(s) 305
Bsp143I GATC 1 cut(s) 11
Bsp68I TCGCGA 1 cut(s) 949
BspACI CCGC 6 cut(s) 153, 401, 461, 630, 698, 824
BspANI GGCC 2 cut(s) 170, 894
BspCNI CTCAG 3 cut(s) 487, 589, 803
BspFNI CGCG 1 cut(s) 949
BspHI TCATGA 1 cut(s) 810
BspLI GGNNCC 1 cut(s) 786
BspMI ACCTGC 1 cut(s) 71
BspPI GGATC 1 cut(s) 6
BspQI GCTCTTC 1 cut(s) 687
BspT104I TTCGAA 1 cut(s) 305
BspT107I GGYRCC 1 cut(s) 784
BsrI ACTGG 1 cut(s) 917
BssECI CCNNGG 1 cut(s) 710
BssMI GATC 1 cut(s) 11
BssT1I CCWWGG 1 cut(s) 710
Bst4CI ACNGT 4 cut(s) 275, 320, 594, 922
Bst6I CTCTTC 2 cut(s) 246, 687
BstBI TTCGAA 1 cut(s) 305
BstC8I GCNNGC 4 cut(s) 192, 281, 288, 951
BstDEI CTNAG 4 cut(s) 495, 572, 597, 790
BstF5I GGATG 2 cut(s) 262, 901
BstFNI CGCG 1 cut(s) 949
BstH2I RGCGCY 1 cut(s) 746
BstHHI GCGC 1 cut(s) 745
BstKTI GATC 1 cut(s) 14
BstMBI GATC 1 cut(s) 11
BstMWI GCNNNNNNNGC 2 cut(s) 677, 950
BstNSI RCATGY 2 cut(s) 283, 290
BstSFI CTRYAG 1 cut(s) 918
BstUI CGCG 1 cut(s) 949
BstV1I GCAGC 2 cut(s) 206, 295
BstV2I GAAGAC 1 cut(s) 513
BstX2I RGATCY 1 cut(s) 11
BstYI RGATCY 1 cut(s) 11
BsuRI GGCC 2 cut(s) 170, 894
BtgZI GCGATG 1 cut(s) 930
BtsCI GGATG 2 cut(s) 262, 901
BtsI GCAGTG 2 cut(s) 134, 495
BtsIMutI CAGTG 4 cut(s) 107, 134, 495, 910
BtuMI TCGCGA 1 cut(s) 949
BveI ACCTGC 1 cut(s) 71
Cac8I GCNNGC 4 cut(s) 192, 281, 288, 951
CaiI CAGNNNCTG 1 cut(s) 494
CciI TCATGA 1 cut(s) 810
CfoI GCGC 1 cut(s) 745
Cfr13I GGNCC 2 cut(s) 226, 892
Csp6I GTAC 3 cut(s) 590, 763, 785
CviAII CATG 5 cut(s) 280, 287, 371, 666, 811
CviQI GTAC 3 cut(s) 590, 763, 785
DdeI CTNAG 4 cut(s) 495, 572, 597, 790
DpnI GATC 1 cut(s) 13
DpnII GATC 1 cut(s) 11
Eam1104I CTCTTC 2 cut(s) 246, 687
EarI CTCTTC 2 cut(s) 246, 687
Eco130I CCWWGG 1 cut(s) 710
Eco47I GGWCC 1 cut(s) 226
Eco47III AGCGCT 1 cut(s) 744
Eco57I CTGAAG 1 cut(s) 468
EcoT14I CCWWGG 1 cut(s) 710
ErhI CCWWGG 1 cut(s) 710
FaeI CATG 5 cut(s) 283, 290, 374, 669, 814
FaqI GGGAC 1 cut(s) 942
FatI CATG 5 cut(s) 279, 286, 370, 665, 810
FauI CCCGC 2 cut(s) 146, 454
FauNDI CATATG 1 cut(s) 730
Fnu4HI GCNGC 2 cut(s) 195, 284
FokI GGATG 2 cut(s) 269, 888
Fsp4HI GCNGC 2 cut(s) 195, 284
FspBI CTAG 2 cut(s) 206, 746
GlaI GCGC 1 cut(s) 744
GluI GCNGC 2 cut(s) 195, 284
GsuI CTGGAG 1 cut(s) 906
HaeII RGCGCY 1 cut(s) 746
HaeIII GGCC 2 cut(s) 170, 894
HhaI GCGC 1 cut(s) 745
Hin1II CATG 5 cut(s) 283, 290, 374, 669, 814
Hin6I GCGC 1 cut(s) 743
HinP1I GCGC 1 cut(s) 743
HindIII AAGCTT 1 cut(s) 603
HinfI GANTC 2 cut(s) 469, 479
HphI GGTGA 2 cut(s) 374, 826
Hpy166II GTNNAC 1 cut(s) 226
Hpy188I TCNGA 3 cut(s) 598, 613, 756
Hpy188III TCNNGA 4 cut(s) 451, 811, 858, 948
Hpy8I GTNNAC 1 cut(s) 226
HpyAV CCTTC 2 cut(s) 521, 616
HpyCH4III ACNGT 4 cut(s) 275, 320, 594, 922
HpyCH4V TGCA 3 cut(s) 194, 283, 671
HpyF10VI GCNNNNNNNGC 2 cut(s) 677, 950
HpyF3I CTNAG 4 cut(s) 495, 572, 597, 790
Hsp92II CATG 5 cut(s) 283, 290, 374, 669, 814
HspAI GCGC 1 cut(s) 743
KpnI GGTACC 1 cut(s) 788
Kzo9I GATC 1 cut(s) 11
LguI GCTCTTC 1 cut(s) 687
LpnPI CCDG 9 cut(s) 29, 66, 124, 224, 398, 436, 870, 898, 926
Lsp1109I GCAGC 2 cut(s) 206, 295
LweI GCATC 3 cut(s) 747, 910, 953
MaeI CTAG 2 cut(s) 206, 746
MaeIII GTNAC 5 cut(s) 83, 328, 380, 908, 922
MalI GATC 1 cut(s) 13
MboI GATC 1 cut(s) 11
MboII GAAGA 7 cut(s) 194, 263, 319, 474, 478, 518, 674
MflI RGATCY 1 cut(s) 11
MluCI AATT 7 cut(s) 5, 66, 180, 301, 389, 818, 864
MmeI TCCRAC 2 cut(s) 276, 355
MnlI CCTC 7 cut(s) 247, 490, 533, 536, 708, 799, 915
MroXI GAANNNNTTC 1 cut(s) 510
MseI TTAA 5 cut(s) 8, 56, 123, 581, 964
MslI CAYNNNNRTG 1 cut(s) 164
Mva1269I GAATGC 2 cut(s) 366, 561
MvnI CGCG 1 cut(s) 949
MwoI GCNNNNNNNGC 2 cut(s) 677, 950
NdeI CATATG 1 cut(s) 730
NdeII GATC 1 cut(s) 11
NlaIII CATG 5 cut(s) 283, 290, 374, 669, 814
NlaIV GGNNCC 1 cut(s) 786
NmuCI GTSAC 3 cut(s) 328, 380, 908
NruI TCGCGA 1 cut(s) 949
NspI RCATGY 2 cut(s) 283, 290
NspV TTCGAA 1 cut(s) 305
PaeI GCATGC 2 cut(s) 283, 290
PagI TCATGA 1 cut(s) 810
PciSI GCTCTTC 1 cut(s) 687
PctI GAATGC 2 cut(s) 366, 561
PdmI GAANNNNTTC 1 cut(s) 510
PfeI GAWTC 2 cut(s) 469, 479
PkrI GCNGC 2 cut(s) 196, 285
PspN4I GGNNCC 1 cut(s) 786
PspPI GGNCC 2 cut(s) 226, 892
PstNI CAGNNNCTG 1 cut(s) 494
PsuI RGATCY 1 cut(s) 11
RruI TCGCGA 1 cut(s) 949
RsaI GTAC 3 cut(s) 591, 764, 786
RsaNI GTAC 3 cut(s) 590, 763, 785
RseI CAYNNNNRTG 1 cut(s) 164
SapI GCTCTTC 1 cut(s) 687
SaqAI TTAA 5 cut(s) 8, 56, 123, 581, 964
SatI GCNGC 2 cut(s) 195, 284
Sau3AI GATC 1 cut(s) 11
Sau96I GGNCC 2 cut(s) 226, 892
SfaNI GCATC 3 cut(s) 747, 910, 953
SfcI CTRYAG 1 cut(s) 918
SfuI TTCGAA 1 cut(s) 305
SinI GGWCC 1 cut(s) 226
SmiMI CAYNNNNRTG 1 cut(s) 164
SphI GCATGC 2 cut(s) 283, 290
Sse9I AATT 7 cut(s) 5, 66, 180, 301, 389, 818, 864
SsiI CCGC 6 cut(s) 153, 401, 461, 630, 698, 824
SspMI CTAG 2 cut(s) 206, 746
StyI CCWWGG 1 cut(s) 710
TaaI ACNGT 4 cut(s) 275, 320, 594, 922
TaqI TCGA 2 cut(s) 51, 305
TasI AATT 7 cut(s) 5, 66, 180, 301, 389, 818, 864
TatI WGTACW 1 cut(s) 762
TfiI GAWTC 2 cut(s) 469, 479
Tru1I TTAA 5 cut(s) 8, 56, 123, 581, 964
Tru9I TTAA 5 cut(s) 8, 56, 123, 581, 964
TscAI CASTG 4 cut(s) 114, 134, 495, 917
TseFI GTSAC 3 cut(s) 328, 380, 908
TseI GCWGC 2 cut(s) 194, 283
Tsp45I GTSAC 3 cut(s) 328, 380, 908
TspDTI ATGAA 9 cut(s) 17, 148, 264, 312, 359, 519, 597, 720, 799
TspRI CASTG 4 cut(s) 114, 134, 495, 917
VpaK11BI GGWCC 1 cut(s) 226
XapI RAATTY 3 cut(s) 180, 301, 389
XceI RCATGY 2 cut(s) 283, 290
XmnI GAANNNNTTC 1 cut(s) 510
XspI CTAG 2 cut(s) 206, 746
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.