Rh5BG101300

Tobamovirus multiplication protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001716
Physical Location & Seq
Reverse (-)
9257439 .. 9263741
6303 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG101300.1

Sequence Viewer

Length: 1086 bp
ATGTTTGAATTGAGAGAAGGGTCTTGTTTGCCAAAGGTGTTGGTGGGTGTGAATGTGGGTCTTGCTCTTGTTGATGCCATTATAGCTGCCCTTGCATTTTATCAGTTAATTAGGATTCACTCGAGGAATTCACAACTTGGATGGACTCGCCAAAAAGTTTTTCATCTGTTGATTGGCTCTTCGAATTTGGCCTCATGGGTATGCTTCCATTCTTATATGCAAGTATGCTACCTATCTTACGTGTTTGCTCAATGTATCTTAACAGTGAGATTTTTCTTTGCAGCTTTACCCAAAATTTTGTTTTTTGCTGCATTCCTTCTACTTTTATCGTTCTGGGTTGACCTGTGCCATCAGGCAGATGACGAAGATGAGGAGGATGAAGGTAGTTTTCAGGAAGCTTTGTTGGAGAAGACATTTAGCAAAACTAATTCATTGGACACTGATAGTCAGAGAAGGTGTTTTCCTCTGCGAATCCATATTGGAAGCCGCCAGAAAATTGTAGTTTTGGTTACAGTGCTAGTTTTTATTATAATGATCGTGTGTGCTGTGATAATCTGGATCGGGATGGGAAAAAATCCCATTGATTCTGCTGTGGTAGCTCGGGTATATGTAGATCTTTTTGCAATAGTAACACTATTATTGGGAGGAGCATTGGCATGTTATGGACTTCTATTATGCTTGAGAATGAGAAAAGTTAGATCTGAGAGAGCCTCTTCTGAGATGTGGAAGGTTGCAGGTTTAGCTGTCGTTTCCGTTCTATGTTTCGCCTCAAGTGCTTTCGTGGCTCTATTAACTGATATTCCTATGCTTTACCATGACTATCAACAGCCTGTGAATGGTGTTTATACTGCACTTTTGCTGATTTTATATTATTTTGTAGGTTCATCAATACCCTCAGCGTATGTATTATGGGTCATGAGAGAATTACCACCCTCAATAGCAGCTAACATACGACAAGAAGAAGAAGAATCAACTACATTAACATTCATCAGTGATGATTCCAACGTATTACCACTTCCTCAGAGCTGGACTGCAGCAATGAGCTTGCGGAACCAGGTGCAGGTATCAAGAGCAAGTCCGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1084

Protein Analysis

361

Amino Acids

40.42

Weight (kDa)

6.31

Isoelectric Point (pI)

44.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
THH1_TOM1-3_dom PF06454 17 - 120 3.5e-15 THH1/TOM1/TOM3 domain
THH1_TOM1-3_dom PF06454 173 - 314 6.1e-09 THH1/TOM1/TOM3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 530
Acc36I ACCTGC 2 cut(s) 725, 1051
AciI CCGC 2 cut(s) 487, 1048
AclWI GGATC 1 cut(s) 566
AcsI RAATTY 3 cut(s) 127, 184, 294
AfiI CCNNNNNNNGG 2 cut(s) 836, 1060
AflIII ACRYGT 1 cut(s) 240
AgsI TTSAA 1 cut(s) 8
AjnI CCWGG 1 cut(s) 1053
AjuI GAANNNNNNNTTGG 2 cut(s) 144, 176
AluBI AGCT 8 cut(s) 86, 284, 398, 599, 743, 944, 1026, 1044
AluI AGCT 8 cut(s) 86, 284, 398, 599, 743, 944, 1026, 1044
AlwI GGATC 1 cut(s) 566
Ama87I CYCGRG 2 cut(s) 121, 600
AoxI GGCC 1 cut(s) 189
ApeKI GCWGC 5 cut(s) 86, 281, 308, 941, 1034
ApoI RAATTY 3 cut(s) 127, 184, 294
AsuII TTCGAA 1 cut(s) 182
AvaI CYCGRG 2 cut(s) 121, 600
BarI GAAGNNNNNNTAC 2 cut(s) 999, 1031
BbsI GAAGAC 1 cut(s) 416
BbvCI CCTCAGC 1 cut(s) 895
BbvI GCAGC 5 cut(s) 73, 293, 295, 953, 1046
BccI CCATC 3 cut(s) 135, 357, 559
BciT130I CCWGG 1 cut(s) 1055
BfaI CTAG 1 cut(s) 518
BfmI CTRYAG 1 cut(s) 1032
BfuAI ACCTGC 2 cut(s) 725, 1051
BglII AGATCT 2 cut(s) 613, 698
BisI GCNGC 6 cut(s) 87, 282, 309, 487, 942, 1035
BlsI GCNGC 6 cut(s) 88, 283, 310, 488, 943, 1036
Bme1390I CCNGG 1 cut(s) 1055
BmeT110I CYCGRG 2 cut(s) 121, 600
BmiI GGNNCC 1 cut(s) 1052
BmrFI CCNGG 1 cut(s) 1055
BmsI GCATC 1 cut(s) 64
BpiI GAAGAC 1 cut(s) 416
BplI GAGNNNNNCTC 2 cut(s) 695, 727
Bpu10I CCTNAGC 1 cut(s) 895
Bpu14I TTCGAA 1 cut(s) 182
BpuEI CTTGAG 2 cut(s) 700, 754
BsaAI YACGTR 1 cut(s) 241
Bsc4I CCNNNNNNNGG 2 cut(s) 836, 1060
Bse3DI GCAATG 1 cut(s) 1044
BseBI CCWGG 1 cut(s) 1055
BseGI GGATG 3 cut(s) 146, 382, 570
BseLI CCNNNNNNNGG 2 cut(s) 836, 1060
BseMI GCAATG 1 cut(s) 1044
BseMII CTCAG 4 cut(s) 693, 708, 909, 1034
BseRI GAGGAG 2 cut(s) 386, 660
BseXI GCAGC 5 cut(s) 73, 293, 295, 953, 1046
BsgI GTGCAG 2 cut(s) 834, 1079
BshFI GGCC 1 cut(s) 191
BsiHKCI CYCGRG 2 cut(s) 121, 600
BslI CCNNNNNNNGG 2 cut(s) 836, 1060
BsmI GAATGC 1 cut(s) 311
BsnI GGCC 1 cut(s) 191
BsoBI CYCGRG 2 cut(s) 121, 600
Bsp119I TTCGAA 1 cut(s) 182
Bsp143I GATC 4 cut(s) 534, 558, 613, 698
BspACI CCGC 2 cut(s) 487, 1048
BspANI GGCC 1 cut(s) 191
BspCNI CTCAG 4 cut(s) 694, 709, 908, 1033
BspHI TCATGA 1 cut(s) 915
BspLI GGNNCC 1 cut(s) 1052
BspMAI CTGCAG 1 cut(s) 1036
BspMI ACCTGC 2 cut(s) 725, 1051
BspPI GGATC 1 cut(s) 566
BspQI GCTCTTC 1 cut(s) 184
BspT104I TTCGAA 1 cut(s) 182
BsrDI GCAATG 1 cut(s) 1044
BssMI GATC 4 cut(s) 534, 558, 613, 698
Bst2UI CCWGG 1 cut(s) 1055
Bst4CI ACNGT 2 cut(s) 265, 514
Bst6I CTCTTC 2 cut(s) 184, 718
BstBAI YACGTR 1 cut(s) 241
BstBI TTCGAA 1 cut(s) 182
BstC8I GCNNGC 1 cut(s) 1046
BstDEI CTNAG 4 cut(s) 702, 717, 895, 1020
BstF5I GGATG 3 cut(s) 146, 382, 570
BstKTI GATC 4 cut(s) 537, 561, 616, 701
BstMBI GATC 4 cut(s) 534, 558, 613, 698
BstMWI GCNNNNNNNGC 6 cut(s) 83, 92, 596, 740, 773, 782
BstNI CCWGG 1 cut(s) 1055
BstNSI RCATGY 1 cut(s) 660
BstSCI CCNGG 1 cut(s) 1053
BstSFI CTRYAG 1 cut(s) 1032
BstV1I GCAGC 5 cut(s) 73, 293, 295, 953, 1046
BstV2I GAAGAC 1 cut(s) 416
BstX2I RGATCY 2 cut(s) 613, 698
BstYI RGATCY 2 cut(s) 613, 698
BsuRI GGCC 1 cut(s) 191
BtsCI GGATG 3 cut(s) 146, 382, 570
BtsIMutI CAGTG 4 cut(s) 270, 438, 519, 997
BveI ACCTGC 2 cut(s) 725, 1051
Cac8I GCNNGC 1 cut(s) 1046
CciI TCATGA 1 cut(s) 915
CsiI ACCWGGT 1 cut(s) 1053
CviAII CATG 4 cut(s) 195, 657, 815, 916
DdeI CTNAG 4 cut(s) 702, 717, 895, 1020
DpnI GATC 4 cut(s) 536, 560, 615, 700
DpnII GATC 4 cut(s) 534, 558, 613, 698
Eam1104I CTCTTC 2 cut(s) 184, 718
EarI CTCTTC 2 cut(s) 184, 718
Eco88I CYCGRG 2 cut(s) 121, 600
EcoRI GAATTC 1 cut(s) 127
EcoRII CCWGG 1 cut(s) 1053
FaeI CATG 4 cut(s) 198, 660, 818, 919
FatI CATG 4 cut(s) 194, 656, 814, 915
Fnu4HI GCNGC 6 cut(s) 87, 282, 309, 487, 942, 1035
FokI GGATG 3 cut(s) 153, 389, 577
Fsp4HI GCNGC 6 cut(s) 87, 282, 309, 487, 942, 1035
FspBI CTAG 1 cut(s) 518
GluI GCNGC 6 cut(s) 87, 282, 309, 487, 942, 1035
HaeIII GGCC 1 cut(s) 191
Hin1II CATG 4 cut(s) 198, 660, 818, 919
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HindIII AAGCTT 1 cut(s) 396
HinfI GANTC 6 cut(s) 115, 145, 471, 584, 968, 998
Hpy166II GTNNAC 1 cut(s) 340
Hpy188I TCNGA 5 cut(s) 450, 703, 718, 1023, 1080
Hpy188III TCNNGA 5 cut(s) 392, 556, 562, 916, 1068
Hpy8I GTNNAC 1 cut(s) 340
HpyAV CCTTC 5 cut(s) 11, 326, 374, 447, 721
HpyCH4III ACNGT 2 cut(s) 265, 514
HpyCH4IV ACGT 2 cut(s) 240, 1005
HpyCH4V TGCA 9 cut(s) 95, 220, 281, 311, 623, 734, 851, 1034, 1060
HpyF10VI GCNNNNNNNGC 6 cut(s) 83, 92, 596, 740, 773, 782
HpyF3I CTNAG 4 cut(s) 702, 717, 895, 1020
HpySE526I ACGT 2 cut(s) 240, 1005
Hsp92II CATG 4 cut(s) 198, 660, 818, 919
Kzo9I GATC 4 cut(s) 534, 558, 613, 698
LguI GCTCTTC 1 cut(s) 184
LmnI GCTCC 1 cut(s) 647
Lsp1109I GCAGC 5 cut(s) 73, 293, 295, 953, 1046
LweI GCATC 1 cut(s) 64
MabI ACCWGGT 1 cut(s) 1053
MaeI CTAG 1 cut(s) 518
MaeII ACGT 2 cut(s) 240, 1005
MaeIII GTNAC 2 cut(s) 508, 628
MalI GATC 4 cut(s) 536, 560, 615, 700
MboI GATC 4 cut(s) 534, 558, 613, 698
MboII GAAGA 7 cut(s) 171, 377, 421, 705, 971, 974, 977
MflI RGATCY 2 cut(s) 613, 698
MluCI AATT 8 cut(s) 8, 108, 127, 184, 294, 427, 495, 923
MlyI GAGTC 1 cut(s) 139
MmeI TCCRAC 2 cut(s) 384, 1026
MseI TTAA 4 cut(s) 107, 260, 791, 980
MslI CAYNNNNRTG 2 cut(s) 199, 655
MspR9I CCNGG 1 cut(s) 1055
Mva1269I GAATGC 1 cut(s) 311
MvaI CCWGG 1 cut(s) 1055
MwoI GCNNNNNNNGC 6 cut(s) 83, 92, 596, 740, 773, 782
NdeII GATC 4 cut(s) 534, 558, 613, 698
NlaIII CATG 4 cut(s) 198, 660, 818, 919
NlaIV GGNNCC 1 cut(s) 1052
NspI RCATGY 1 cut(s) 660
NspV TTCGAA 1 cut(s) 182
PaeR7I CTCGAG 1 cut(s) 121
PagI TCATGA 1 cut(s) 915
PciSI GCTCTTC 1 cut(s) 184
PctI GAATGC 1 cut(s) 311
PfeI GAWTC 5 cut(s) 115, 471, 584, 968, 998
PkrI GCNGC 6 cut(s) 88, 283, 310, 488, 943, 1036
PleI GAGTC 1 cut(s) 139
PpsI GAGTC 1 cut(s) 139
Ppu21I YACGTR 1 cut(s) 241
PsiI TTATAA 1 cut(s) 530
Psp6I CCWGG 1 cut(s) 1053
PspGI CCWGG 1 cut(s) 1053
PspN4I GGNNCC 1 cut(s) 1052
PspXI VCTCGAGB 1 cut(s) 121
PstI CTGCAG 1 cut(s) 1036
PsuI RGATCY 2 cut(s) 613, 698
RseI CAYNNNNRTG 2 cut(s) 199, 655
SapI GCTCTTC 1 cut(s) 184
SaqAI TTAA 4 cut(s) 107, 260, 791, 980
SatI GCNGC 6 cut(s) 87, 282, 309, 487, 942, 1035
Sau3AI GATC 4 cut(s) 534, 558, 613, 698
SchI GAGTC 1 cut(s) 139
ScrFI CCNGG 1 cut(s) 1055
SexAI ACCWGGT 1 cut(s) 1053
SfaNI GCATC 1 cut(s) 64
SfcI CTRYAG 1 cut(s) 1032
Sfr274I CTCGAG 1 cut(s) 121
SfuI TTCGAA 1 cut(s) 182
SlaI CTCGAG 1 cut(s) 121
SmiMI CAYNNNNRTG 2 cut(s) 199, 655
SmlI CTYRAG 3 cut(s) 121, 679, 769
SmoI CTYRAG 3 cut(s) 121, 679, 769
Sse9I AATT 8 cut(s) 8, 108, 127, 184, 294, 427, 495, 923
SsiI CCGC 2 cut(s) 487, 1048
SspMI CTAG 1 cut(s) 518
StyD4I CCNGG 1 cut(s) 1053
TaaI ACNGT 2 cut(s) 265, 514
TaiI ACGT 2 cut(s) 243, 1008
TaqI TCGA 2 cut(s) 122, 182
TasI AATT 8 cut(s) 8, 108, 127, 184, 294, 427, 495, 923
TauI GCSGC 1 cut(s) 489
TfiI GAWTC 5 cut(s) 115, 471, 584, 968, 998
Tru1I TTAA 4 cut(s) 107, 260, 791, 980
Tru9I TTAA 4 cut(s) 107, 260, 791, 980
TscAI CASTG 4 cut(s) 270, 445, 519, 997
TseI GCWGC 5 cut(s) 86, 281, 308, 941, 1034
TspDTI ATGAA 5 cut(s) 152, 393, 420, 873, 976
TspGWI ACGGA 1 cut(s) 742
TspRI CASTG 4 cut(s) 270, 445, 519, 997
XapI RAATTY 3 cut(s) 127, 184, 294
XceI RCATGY 1 cut(s) 660
XhoI CTCGAG 1 cut(s) 121
XspI CTAG 1 cut(s) 518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.