Rroxscaffold_2G00096180

Tobamovirus multiplication protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001716
Physical Location & Seq
Forward (+)
17414854 .. 17420067
5214 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00096180.1

Sequence Viewer

Length: 1092 bp
ATGATGTTGTTGGAATCAATACTGGAAAAAAGTCGTTTCTTTCGTTTGGACTTGCCGTTGTTTATCCTCAATATAGCTCTGGCCGTTTTCAATGGCGTTCTTGCTGCGATTTCGTTTTCTCAGCTGGCCAGGATTCACTTGCGCAATCAACAAATTGGATGGACACGCCAAAAAGTACTCCACCTCATGATTGGCTGTTCTAACTTGGGTTACTTCCTATATTTTACATCTACACTGATTGCCACTTTTAAGCAGTGGTTTTGCTGGTCTAATGTCTGCGGGTTCATTCTAATGGCCTACCCTAAAATTCTGTTGCTTGCAGCTTTTCTTCTAGTCCTATCCTTTTGGGTGGACCTTTGCCATCAGGCAAATGATGAAGAGGATGATGATGATGATGACAGTAGCATGCAGCATGCCTTGTTGGAAAATTCGAAGAATAAACACCGTTCATCAGTCACAAATGGTCATAGGATTTGTTGCTCATTCCCAAGCATTCATGTTGGAAGTCACCAGAAATTTGTGATTGCGGTTGTTGGGTTTGTCCTTATTTTGATGATGTCATTTTCTGTGGTAATCTGGATTGGTGCGGGGAAGAATCCCATTGATTCTTCAGCAGTGGCTGAGGTATATGAAGACCTTCTCGCCTTTGTAGTTCTTTTTTTAGGAGGAGGATTAGGTTGCTATGGCATTCTGCTACTTCGTAAGTTAAGGAAAGTACGGTCTGAGAAAGCTTCATCGGAAATGTGGAAGGTTGGCGGTTTAGCAGTTGCTTCTGTTGTATGTTTTACATCAAGTGCATTAGTAGCTCTTCTTACAGATATTCCGCTATATGAACCTTGGAGGCTGAAAAAAGCATATGGTGGGAAAGCACTAGTTTTTCTGATGCTATACTATTTTATAGGCTGTTGGGTACCCTCAGCTTTTGTATTATGGTTCATGAGAGAATTACCGCCACCAATCACTAATCGCCAACGGGAACAATCAAGAACAATTACTTTTATCAGCTATGCTGGAGTGGGCCGACAGCATCCTCGTCACTGGGCTACAGTAACAAGTTCAAATAATCAGGCATCGCGAGCAAGTCCCATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1084

Protein Analysis

363

Amino Acids

40.86

Weight (kDa)

9.12

Isoelectric Point (pI)

38.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
THH1_TOM1-3_dom PF06454 24 - 125 1.1e-10 THH1/TOM1/TOM3 domain
THH1_TOM1-3_dom PF06454 184 - 325 3.9e-06 THH1/TOM1/TOM3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 143
Acc65I GGTACC 1 cut(s) 910
AccB1I GGYRCC 1 cut(s) 910
AccII CGCG 1 cut(s) 1075
AciI CCGC 6 cut(s) 279, 527, 587, 756, 824, 950
AcoI YGGCCR 2 cut(s) 81, 126
AcsI RAATTY 3 cut(s) 306, 427, 515
AcuI CTGAAG 1 cut(s) 594
AfaI GTAC 3 cut(s) 177, 717, 912
AgsI TTSAA 2 cut(s) 91, 1059
AhlI ACTAGT 1 cut(s) 871
AjnI CCWGG 1 cut(s) 128
AluBI AGCT 7 cut(s) 77, 124, 323, 731, 806, 920, 1005
AluI AGCT 7 cut(s) 77, 124, 323, 731, 806, 920, 1005
AlwNI CAGNNNCTG 1 cut(s) 620
AoxI GGCC 4 cut(s) 81, 126, 294, 1018
ApeKI GCWGC 3 cut(s) 104, 320, 409
ApoI RAATTY 3 cut(s) 306, 427, 515
Asp700I GAANNNNTTC 1 cut(s) 636
Asp718I GGTACC 1 cut(s) 910
AspLEI GCGC 1 cut(s) 144
AspS9I GGNCC 2 cut(s) 352, 1018
AsuHPI GGTGA 1 cut(s) 500
AsuII TTCGAA 1 cut(s) 431
AvaII GGWCC 1 cut(s) 352
BalI TGGCCA 1 cut(s) 128
BanI GGYRCC 1 cut(s) 910
BbsI GAAGAC 1 cut(s) 639
BbvCI CCTCAGC 2 cut(s) 621, 916
BbvI GCAGC 3 cut(s) 91, 332, 421
BccI CCATC 2 cut(s) 153, 369
BceAI ACGGC 2 cut(s) 40, 68
BciT130I CCWGG 1 cut(s) 130
BcuI ACTAGT 1 cut(s) 871
BfaI CTAG 2 cut(s) 332, 872
BfmI CTRYAG 1 cut(s) 1044
BisI GCNGC 3 cut(s) 105, 321, 410
BlsI GCNGC 3 cut(s) 106, 322, 411
BmcAI AGTACT 1 cut(s) 177
Bme1390I CCNGG 1 cut(s) 130
Bme18I GGWCC 1 cut(s) 352
BmgT120I GGNCC 2 cut(s) 352, 1018
BmiI GGNNCC 1 cut(s) 912
BmrFI CCNGG 1 cut(s) 130
BmrI ACTGGG 1 cut(s) 1048
BmsI GCATC 3 cut(s) 873, 1036, 1079
BmuI ACTGGG 1 cut(s) 1048
BpiI GAAGAC 1 cut(s) 639
BpmI CTGGAG 1 cut(s) 1032
Bpu10I CCTNAGC 2 cut(s) 621, 916
Bpu14I TTCGAA 1 cut(s) 431
BsaJI CCNNGG 1 cut(s) 836
Bse1I ACTGG 2 cut(s) 27, 1043
BseBI CCWGG 1 cut(s) 130
BseDI CCNNGG 1 cut(s) 836
BseGI GGATG 3 cut(s) 164, 388, 1027
BseMII CTCAG 4 cut(s) 134, 612, 714, 930
BseNI ACTGG 2 cut(s) 27, 1043
BseRI GAGGAG 1 cut(s) 681
BseXI GCAGC 3 cut(s) 91, 332, 421
Bsh1236I CGCG 1 cut(s) 1075
BshFI GGCC 4 cut(s) 83, 128, 296, 1020
BshNI GGYRCC 1 cut(s) 910
BslFI GGGAC 1 cut(s) 1068
BsmFI GGGAC 1 cut(s) 1068
BsmI GAATGC 2 cut(s) 492, 687
BsnI GGCC 4 cut(s) 83, 128, 296, 1020
Bsp119I TTCGAA 1 cut(s) 431
Bsp68I TCGCGA 1 cut(s) 1075
BspACI CCGC 6 cut(s) 279, 527, 587, 756, 824, 950
BspANI GGCC 4 cut(s) 83, 128, 296, 1020
BspCNI CTCAG 4 cut(s) 133, 613, 715, 929
BspFNI CGCG 1 cut(s) 1075
BspHI TCATGA 2 cut(s) 186, 936
BspLI GGNNCC 1 cut(s) 912
BspQI GCTCTTC 1 cut(s) 813
BspT104I TTCGAA 1 cut(s) 431
BspT107I GGYRCC 1 cut(s) 910
BsrI ACTGG 2 cut(s) 27, 1043
BssECI CCNNGG 1 cut(s) 836
BssT1I CCWWGG 1 cut(s) 836
Bst2UI CCWGG 1 cut(s) 130
Bst4CI ACNGT 4 cut(s) 401, 446, 720, 1048
Bst6I CTCTTC 2 cut(s) 372, 813
BstBI TTCGAA 1 cut(s) 431
BstC8I GCNNGC 5 cut(s) 126, 318, 407, 414, 1077
BstDEI CTNAG 4 cut(s) 120, 621, 723, 916
BstF5I GGATG 3 cut(s) 164, 388, 1027
BstFNI CGCG 1 cut(s) 1075
BstHHI GCGC 1 cut(s) 144
BstMWI GCNNNNNNNGC 2 cut(s) 803, 1076
BstNI CCWGG 1 cut(s) 130
BstNSI RCATGY 2 cut(s) 409, 416
BstSCI CCNGG 1 cut(s) 128
BstSFI CTRYAG 1 cut(s) 1044
BstUI CGCG 1 cut(s) 1075
BstV1I GCAGC 3 cut(s) 91, 332, 421
BstV2I GAAGAC 1 cut(s) 639
BsuRI GGCC 4 cut(s) 83, 128, 296, 1020
BtgZI GCGATG 1 cut(s) 1056
BtsCI GGATG 3 cut(s) 164, 388, 1027
BtsI GCAGTG 2 cut(s) 260, 621
BtsIMutI CAGTG 4 cut(s) 233, 260, 621, 1036
BtuMI TCGCGA 1 cut(s) 1075
Cac8I GCNNGC 5 cut(s) 126, 318, 407, 414, 1077
CaiI CAGNNNCTG 1 cut(s) 620
CciI TCATGA 2 cut(s) 186, 936
CfoI GCGC 1 cut(s) 144
Cfr13I GGNCC 2 cut(s) 352, 1018
Csp6I GTAC 3 cut(s) 176, 716, 911
CviAII CATG 5 cut(s) 187, 406, 413, 497, 937
CviQI GTAC 3 cut(s) 176, 716, 911
DdeI CTNAG 4 cut(s) 120, 621, 723, 916
EaeI YGGCCR 2 cut(s) 81, 126
Eam1104I CTCTTC 2 cut(s) 372, 813
EarI CTCTTC 2 cut(s) 372, 813
Eco130I CCWWGG 1 cut(s) 836
Eco47I GGWCC 1 cut(s) 352
Eco57I CTGAAG 1 cut(s) 594
EcoRII CCWGG 1 cut(s) 128
EcoT14I CCWWGG 1 cut(s) 836
ErhI CCWWGG 1 cut(s) 836
FaeI CATG 5 cut(s) 190, 409, 416, 500, 940
FaqI GGGAC 1 cut(s) 1068
FatI CATG 5 cut(s) 186, 405, 412, 496, 936
FauI CCCGC 2 cut(s) 272, 580
FauNDI CATATG 1 cut(s) 856
Fnu4HI GCNGC 3 cut(s) 105, 321, 410
FokI GGATG 3 cut(s) 171, 395, 1014
Fsp4HI GCNGC 3 cut(s) 105, 321, 410
FspBI CTAG 2 cut(s) 332, 872
FspI TGCGCA 1 cut(s) 143
GlaI GCGC 1 cut(s) 143
GluI GCNGC 3 cut(s) 105, 321, 410
GsuI CTGGAG 1 cut(s) 1032
HaeIII GGCC 4 cut(s) 83, 128, 296, 1020
HhaI GCGC 1 cut(s) 144
Hin1II CATG 5 cut(s) 190, 409, 416, 500, 940
Hin6I GCGC 1 cut(s) 142
HinP1I GCGC 1 cut(s) 142
HindIII AAGCTT 1 cut(s) 729
HinfI GANTC 4 cut(s) 14, 133, 595, 605
HphI GGTGA 1 cut(s) 500
Hpy166II GTNNAC 1 cut(s) 352
Hpy188I TCNGA 3 cut(s) 724, 739, 882
Hpy188III TCNNGA 5 cut(s) 187, 577, 937, 984, 1074
Hpy8I GTNNAC 1 cut(s) 352
HpyAV CCTTC 2 cut(s) 647, 742
HpyCH4III ACNGT 4 cut(s) 401, 446, 720, 1048
HpyCH4V TGCA 3 cut(s) 320, 409, 797
HpyF10VI GCNNNNNNNGC 2 cut(s) 803, 1076
HpyF3I CTNAG 4 cut(s) 120, 621, 723, 916
Hsp92II CATG 5 cut(s) 190, 409, 416, 500, 940
HspAI GCGC 1 cut(s) 142
KpnI GGTACC 1 cut(s) 914
LguI GCTCTTC 1 cut(s) 813
Lsp1109I GCAGC 3 cut(s) 91, 332, 421
LweI GCATC 3 cut(s) 873, 1036, 1079
MaeI CTAG 2 cut(s) 332, 872
MaeIII GTNAC 5 cut(s) 209, 454, 506, 1034, 1048
MboII GAAGA 7 cut(s) 320, 389, 445, 600, 604, 644, 800
MlsI TGGCCA 1 cut(s) 128
MluCI AATT 6 cut(s) 153, 306, 427, 515, 944, 990
MluNI TGGCCA 1 cut(s) 128
MmeI TCCRAC 2 cut(s) 402, 481
MnlI CCTC 9 cut(s) 77, 194, 373, 616, 659, 662, 834, 925, 1041
Mox20I TGGCCA 1 cut(s) 128
MroXI GAANNNNTTC 1 cut(s) 636
MscI TGGCCA 1 cut(s) 128
MseI TTAA 3 cut(s) 249, 707, 1090
MslI CAYNNNNRTG 1 cut(s) 290
Msp20I TGGCCA 1 cut(s) 128
MspA1I CMGCKG 1 cut(s) 124
MspR9I CCNGG 1 cut(s) 130
Mva1269I GAATGC 2 cut(s) 492, 687
MvaI CCWGG 1 cut(s) 130
MvnI CGCG 1 cut(s) 1075
MwoI GCNNNNNNNGC 2 cut(s) 803, 1076
NdeI CATATG 1 cut(s) 856
NlaIII CATG 5 cut(s) 190, 409, 416, 500, 940
NlaIV GGNNCC 1 cut(s) 912
NmuCI GTSAC 3 cut(s) 454, 506, 1034
NruI TCGCGA 1 cut(s) 1075
NsbI TGCGCA 1 cut(s) 143
NspI RCATGY 2 cut(s) 409, 416
NspV TTCGAA 1 cut(s) 431
PaeI GCATGC 2 cut(s) 409, 416
PagI TCATGA 2 cut(s) 186, 936
PciSI GCTCTTC 1 cut(s) 813
PcsI WCGNNNNNNNCGW 1 cut(s) 40
PctI GAATGC 2 cut(s) 492, 687
PdmI GAANNNNTTC 1 cut(s) 636
PfeI GAWTC 4 cut(s) 14, 133, 595, 605
PkrI GCNGC 3 cut(s) 106, 322, 411
Psp6I CCWGG 1 cut(s) 128
PspGI CCWGG 1 cut(s) 128
PspN4I GGNNCC 1 cut(s) 912
PspPI GGNCC 2 cut(s) 352, 1018
PstNI CAGNNNCTG 1 cut(s) 620
PvuII CAGCTG 1 cut(s) 124
RruI TCGCGA 1 cut(s) 1075
RsaI GTAC 3 cut(s) 177, 717, 912
RsaNI GTAC 3 cut(s) 176, 716, 911
RseI CAYNNNNRTG 1 cut(s) 290
SapI GCTCTTC 1 cut(s) 813
SaqAI TTAA 3 cut(s) 249, 707, 1090
SatI GCNGC 3 cut(s) 105, 321, 410
Sau96I GGNCC 2 cut(s) 352, 1018
ScaI AGTACT 1 cut(s) 177
ScrFI CCNGG 1 cut(s) 130
SfaNI GCATC 3 cut(s) 873, 1036, 1079
SfcI CTRYAG 1 cut(s) 1044
SfuI TTCGAA 1 cut(s) 431
SinI GGWCC 1 cut(s) 352
SmiMI CAYNNNNRTG 1 cut(s) 290
SpeI ACTAGT 1 cut(s) 871
SphI GCATGC 2 cut(s) 409, 416
Sse9I AATT 6 cut(s) 153, 306, 427, 515, 944, 990
SsiI CCGC 6 cut(s) 279, 527, 587, 756, 824, 950
SspMI CTAG 2 cut(s) 332, 872
StyD4I CCNGG 1 cut(s) 128
StyI CCWWGG 1 cut(s) 836
TaaI ACNGT 4 cut(s) 401, 446, 720, 1048
TaqI TCGA 1 cut(s) 431
TasI AATT 6 cut(s) 153, 306, 427, 515, 944, 990
TatI WGTACW 1 cut(s) 175
TfiI GAWTC 4 cut(s) 14, 133, 595, 605
Tru1I TTAA 3 cut(s) 249, 707, 1090
Tru9I TTAA 3 cut(s) 249, 707, 1090
TscAI CASTG 4 cut(s) 240, 260, 621, 1043
TseFI GTSAC 3 cut(s) 454, 506, 1034
TseI GCWGC 3 cut(s) 104, 320, 409
Tsp45I GTSAC 3 cut(s) 454, 506, 1034
TspDTI ATGAA 8 cut(s) 274, 390, 438, 485, 645, 723, 846, 925
TspRI CASTG 4 cut(s) 240, 260, 621, 1043
VpaK11BI GGWCC 1 cut(s) 352
XapI RAATTY 3 cut(s) 306, 427, 515
XceI RCATGY 2 cut(s) 409, 416
XcmI CCANNNNNNNNNTGG 1 cut(s) 188
XmnI GAANNNNTTC 1 cut(s) 636
XspI CTAG 2 cut(s) 332, 872
ZrmI AGTACT 1 cut(s) 177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.