MD12G1081700.v1.1

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0005549
Physical Location & Seq
Forward (+)
9979204 .. 9980201
998 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1081700.v1.1.491

Sequence Viewer

Length: 504 bp
ATGAAAAGGCCCCCAGTTGGGAGGGGTACCAGCTTCATCACCAAGGCTATGTGGAGAAATATCATTGATCAGAGTATCTATCAACTGGCTGTCCTTGGAGTTCTCAATTTCTCTGGGGAGAAGCTACTAGGACTTACTGGTTCAGATGCAACTGAGGTTCTCAACACTGTGATATTCAACGCATTTGTGTTTTGCCAGGTGTTCAATCAGATAAACAGTCGTGACATAGAGAAGATAAACATATTCCGTGGAATGTTCGACAGTTGGGTATTTCTAATCGTCATGGTTTGCACAGCGGCCTTCCAGGTTATCATAGTAGAGTTCTTGGGAGCTTTTGCTAGCACTGTGCCACTAAGCTGGCAACTATGGTTACTCAGCATTTTACTTGGAGCAGTTAGCATGCTTGTTGCAGTTGTTCTCAAATTAATACCTGTTGAAAGGATAACTAAGCACCATGACGGATATGAAGCGCTTCCTTCCGGTCCTCCAGAGGGCATAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009624 GO:0009628 GO:0009705 GO:0010035 GO:0010941 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0042742 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055081 GO:0055085 GO:0060548 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098542 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

168

Amino Acids

18.5

Weight (kDa)

6.72

Isoelectric Point (pI)

29.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_ATPase_C PF00689 1 - 143 2.4e-26 Cation transporting ATPase, C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 26
AccB1I GGYRCC 1 cut(s) 26
AciI CCGC 1 cut(s) 296
AfaI GTAC 1 cut(s) 28
AfeI AGCGCT 1 cut(s) 471
AfiI CCNNNNNNNGG 3 cut(s) 17, 18, 491
AgsI TTSAA 3 cut(s) 178, 205, 437
AjnI CCWGG 2 cut(s) 195, 303
AluBI AGCT 4 cut(s) 33, 124, 332, 357
AluI AGCT 4 cut(s) 33, 124, 332, 357
Aor51HI AGCGCT 1 cut(s) 471
AoxI GGCC 2 cut(s) 8, 297
AseI ATTAAT 1 cut(s) 425
Asp700I GAANNNNTTC 1 cut(s) 471
Asp718I GGTACC 1 cut(s) 26
AspLEI GCGC 1 cut(s) 472
AspS9I GGNCC 2 cut(s) 9, 482
AsuHPI GGTGA 1 cut(s) 31
AsuNHI GCTAGC 1 cut(s) 338
AvaII GGWCC 1 cut(s) 482
BanI GGYRCC 1 cut(s) 26
BciT130I CCWGG 2 cut(s) 197, 305
BclI TGATCA 1 cut(s) 67
BfaI CTAG 2 cut(s) 128, 339
BfoI RGCGCY 1 cut(s) 473
BisI GCNGC 1 cut(s) 297
BlsI GCNGC 1 cut(s) 298
Bme1390I CCNGG 2 cut(s) 197, 305
Bme18I GGWCC 1 cut(s) 482
BmgT120I GGNCC 2 cut(s) 9, 482
BmiI GGNNCC 2 cut(s) 11, 28
BmrFI CCNGG 2 cut(s) 197, 305
BmrI ACTGGG 1 cut(s) 8
BmsI GCATC 1 cut(s) 136
BmtI GCTAGC 1 cut(s) 342
BmuI ACTGGG 1 cut(s) 8
BpmI CTGGAG 1 cut(s) 471
BsaJI CCNNGG 3 cut(s) 42, 94, 247
BsaWI WCCGGW 1 cut(s) 479
Bsc4I CCNNNNNNNGG 3 cut(s) 17, 18, 491
Bse1I ACTGG 3 cut(s) 14, 90, 142
BseBI CCWGG 2 cut(s) 197, 305
BseDI CCNNGG 3 cut(s) 42, 94, 247
BseLI CCNNNNNNNGG 3 cut(s) 17, 18, 491
BseMII CTCAG 2 cut(s) 144, 388
BseNI ACTGG 3 cut(s) 14, 90, 142
BshFI GGCC 2 cut(s) 10, 299
BshNI GGYRCC 1 cut(s) 26
BsiSI CCGG 1 cut(s) 480
BslI CCNNNNNNNGG 3 cut(s) 17, 18, 491
BsnI GGCC 2 cut(s) 10, 299
Bsp143I GATC 1 cut(s) 67
BspACI CCGC 1 cut(s) 296
BspANI GGCC 2 cut(s) 10, 299
BspCNI CTCAG 2 cut(s) 145, 387
BspLI GGNNCC 2 cut(s) 11, 28
BspOI GCTAGC 1 cut(s) 342
BspT107I GGYRCC 1 cut(s) 26
BsrI ACTGG 3 cut(s) 14, 90, 142
BssECI CCNNGG 3 cut(s) 42, 94, 247
BssMI GATC 1 cut(s) 67
BssT1I CCWWGG 2 cut(s) 42, 94
Bst2UI CCWGG 2 cut(s) 197, 305
Bst4CI ACNGT 4 cut(s) 169, 218, 263, 346
BstC8I GCNNGC 3 cut(s) 340, 359, 401
BstDEI CTNAG 4 cut(s) 153, 353, 374, 447
BstDSI CCRYGG 1 cut(s) 247
BstH2I RGCGCY 1 cut(s) 473
BstHHI GCGC 1 cut(s) 472
BstKTI GATC 1 cut(s) 70
BstMBI GATC 1 cut(s) 67
BstNI CCWGG 2 cut(s) 197, 305
BstNSI RCATGY 1 cut(s) 403
BstSCI CCNGG 2 cut(s) 195, 303
BstXI CCANNNNNNTGG 1 cut(s) 357
BsuRI GGCC 2 cut(s) 10, 299
BtgI CCRYGG 1 cut(s) 247
BtsIMutI CAGTG 2 cut(s) 165, 342
Cac8I GCNNGC 3 cut(s) 340, 359, 401
CfoI GCGC 1 cut(s) 472
Cfr13I GGNCC 2 cut(s) 9, 482
Csp6I GTAC 1 cut(s) 27
CviAII CATG 3 cut(s) 283, 400, 455
CviJI RGCY 8 cut(s) 10, 33, 47, 89, 124, 299, 332, 357
CviKI_1 RGCY 8 cut(s) 10, 33, 47, 89, 124, 299, 332, 357
CviQI GTAC 1 cut(s) 27
DdeI CTNAG 4 cut(s) 153, 353, 374, 447
DpnI GATC 1 cut(s) 69
DpnII GATC 1 cut(s) 67
Eco130I CCWWGG 2 cut(s) 42, 94
Eco47I GGWCC 1 cut(s) 482
Eco47III AGCGCT 1 cut(s) 471
EcoO109I RGGNCCY 1 cut(s) 9
EcoRII CCWGG 2 cut(s) 195, 303
EcoT14I CCWWGG 2 cut(s) 42, 94
ErhI CCWWGG 2 cut(s) 42, 94
FaeI CATG 3 cut(s) 286, 403, 458
FatI CATG 3 cut(s) 282, 399, 454
FbaI TGATCA 1 cut(s) 67
Fnu4HI GCNGC 1 cut(s) 297
Fsp4HI GCNGC 1 cut(s) 297
FspBI CTAG 2 cut(s) 128, 339
GlaI GCGC 1 cut(s) 471
GluI GCNGC 1 cut(s) 297
GsuI CTGGAG 1 cut(s) 471
HaeII RGCGCY 1 cut(s) 473
HaeIII GGCC 2 cut(s) 10, 299
HapII CCGG 1 cut(s) 480
HhaI GCGC 1 cut(s) 472
Hin1II CATG 3 cut(s) 286, 403, 458
Hin6I GCGC 1 cut(s) 470
HinP1I GCGC 1 cut(s) 470
HpaII CCGG 1 cut(s) 480
HphI GGTGA 1 cut(s) 31
Hpy188I TCNGA 3 cut(s) 72, 145, 210
Hpy188III TCNNGA 2 cut(s) 221, 488
HpyAV CCTTC 2 cut(s) 310, 486
HpyCH4III ACNGT 4 cut(s) 169, 218, 263, 346
HpyCH4V TGCA 3 cut(s) 149, 291, 410
HpyF3I CTNAG 4 cut(s) 153, 353, 374, 447
Hsp92II CATG 3 cut(s) 286, 403, 458
HspAI GCGC 1 cut(s) 470
KpnI GGTACC 1 cut(s) 30
Ksp22I TGATCA 1 cut(s) 67
Kzo9I GATC 1 cut(s) 67
LmnI GCTCC 2 cut(s) 329, 389
LweI GCATC 1 cut(s) 136
MaeI CTAG 2 cut(s) 128, 339
MaeIII GTNAC 2 cut(s) 221, 369
MalI GATC 1 cut(s) 69
MboI GATC 1 cut(s) 67
MboII GAAGA 1 cut(s) 244
MluCI AATT 2 cut(s) 106, 422
MnlI CCTC 4 cut(s) 15, 148, 484, 495
MroXI GAANNNNTTC 1 cut(s) 471
MseI TTAA 1 cut(s) 425
MspA1I CMGCKG 1 cut(s) 296
MspI CCGG 1 cut(s) 480
MspR9I CCNGG 2 cut(s) 197, 305
MvaI CCWGG 2 cut(s) 197, 305
NdeII GATC 1 cut(s) 67
NheI GCTAGC 1 cut(s) 338
NlaIII CATG 3 cut(s) 286, 403, 458
NlaIV GGNNCC 2 cut(s) 11, 28
NmuCI GTSAC 1 cut(s) 221
NspI RCATGY 1 cut(s) 403
PaeI GCATGC 1 cut(s) 403
PdmI GAANNNNTTC 1 cut(s) 471
PkrI GCNGC 1 cut(s) 298
PshBI ATTAAT 1 cut(s) 425
Psp6I CCWGG 2 cut(s) 195, 303
PspGI CCWGG 2 cut(s) 195, 303
PspN4I GGNNCC 2 cut(s) 11, 28
PspPI GGNCC 2 cut(s) 9, 482
RsaI GTAC 1 cut(s) 28
RsaNI GTAC 1 cut(s) 27
SaqAI TTAA 1 cut(s) 425
SatI GCNGC 1 cut(s) 297
Sau3AI GATC 1 cut(s) 67
Sau96I GGNCC 2 cut(s) 9, 482
ScrFI CCNGG 2 cut(s) 197, 305
SetI ASST 8 cut(s) 35, 126, 159, 201, 309, 334, 359, 433
SfaNI GCATC 1 cut(s) 136
SinI GGWCC 1 cut(s) 482
SphI GCATGC 1 cut(s) 403
Sse9I AATT 2 cut(s) 106, 422
SsiI CCGC 1 cut(s) 296
SspMI CTAG 2 cut(s) 128, 339
StyD4I CCNGG 2 cut(s) 195, 303
StyI CCWWGG 2 cut(s) 42, 94
TaaI ACNGT 4 cut(s) 169, 218, 263, 346
TaqI TCGA 1 cut(s) 258
TasI AATT 2 cut(s) 106, 422
TauI GCSGC 1 cut(s) 299
Tru1I TTAA 1 cut(s) 425
Tru9I TTAA 1 cut(s) 425
TscAI CASTG 2 cut(s) 172, 349
TseFI GTSAC 1 cut(s) 221
Tsp45I GTSAC 1 cut(s) 221
TspDTI ATGAA 3 cut(s) 17, 25, 480
TspGWI ACGGA 2 cut(s) 236, 474
TspRI CASTG 2 cut(s) 172, 349
VpaK11BI GGWCC 1 cut(s) 482
VspI ATTAAT 1 cut(s) 425
XceI RCATGY 1 cut(s) 403
XmnI GAANNNNTTC 1 cut(s) 471
XspI CTAG 2 cut(s) 128, 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.