Rw3G022970

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0005549
Physical Location & Seq
Forward (+)
28558728 .. 28559564
837 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G022970.1

Sequence Viewer

Length: 543 bp
ATGTACACTGTTGGTGCACGTGCACTTATTCGAATGCCCCAGCTTTTGAAGGGTGCAAGCTTCATCACCAGGGGCATTTGGAGGAATATCATTGGTCAGAGTATCTATCAACCGACTGTCCTTGGATTTCTCAATTTCTATGGGACGCAGCTACTAGGACTTACTAGTCCAGATGCAATTGCTGTTCTTGACACTGTGATATTCAACTCAATAGTGTTTTGCAAGGTGATTAATGAGATGGTTCAGATGAATAGGCATGAAATAGAGATGATAAACACATTTGACAACTGGATATTGGTAGGATTCATGATTTGCACTTTGGCCTTCGAAGTTGTCATAGTTGAGTTCTTGGGAGTGGTTGCTAGCATTGTGCCAATATGGGTACTGGGCATTTTTCTTGGATCAGTTAGCATGACTGTTGCAGTTGTCCTGGAGTGCACGCCTGTTGGAAGTTCAGCCAAAGCCACAGAAGGACTGGAAATTCATAGATTCTCCGCTGAGCAGCTGAATGCTGTTATGAAGTTGGTCCAGGTTAGTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009624 GO:0009628 GO:0009705 GO:0010035 GO:0010941 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0042742 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055081 GO:0055085 GO:0060548 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098542 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

180

Amino Acids

19.83

Weight (kDa)

6.09

Isoelectric Point (pI)

32.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_ATPase_C PF00689 13 - 140 3.9e-10 Cation transporting ATPase, C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 165
AciI CCGC 1 cut(s) 495
AclWI GGATC 1 cut(s) 409
AcsI RAATTY 1 cut(s) 480
AcvI CACGTG 1 cut(s) 20
AfaI GTAC 2 cut(s) 5, 384
AgsI TTSAA 2 cut(s) 49, 205
AhlI ACTAGT 1 cut(s) 164
AjnI CCWGG 3 cut(s) 68, 429, 528
AluBI AGCT 4 cut(s) 43, 60, 151, 505
AluI AGCT 4 cut(s) 43, 60, 151, 505
Alw21I GWGCWC 3 cut(s) 19, 25, 440
Alw44I GTGCAC 3 cut(s) 15, 21, 436
AlwI GGATC 1 cut(s) 409
AoxI GGCC 1 cut(s) 321
ApaLI GTGCAC 3 cut(s) 15, 21, 436
ApeKI GCWGC 2 cut(s) 148, 502
ApoI RAATTY 1 cut(s) 480
AseI ATTAAT 1 cut(s) 231
AspS9I GGNCC 1 cut(s) 526
AsuHPI GGTGA 2 cut(s) 58, 238
AsuII TTCGAA 2 cut(s) 31, 327
AsuNHI GCTAGC 1 cut(s) 362
AvaII GGWCC 1 cut(s) 526
BaeGI GKGCMC 3 cut(s) 19, 25, 440
BbrPI CACGTG 1 cut(s) 20
Bbv12I GWGCWC 3 cut(s) 19, 25, 440
BbvI GCAGC 2 cut(s) 160, 514
BccI CCATC 1 cut(s) 232
BciT130I CCWGG 3 cut(s) 70, 431, 530
BcuI ACTAGT 1 cut(s) 164
BfaI CTAG 3 cut(s) 155, 165, 363
BisI GCNGC 2 cut(s) 149, 503
BlpI GCTNAGC 1 cut(s) 498
BlsI GCNGC 2 cut(s) 150, 504
Bme1390I CCNGG 3 cut(s) 70, 431, 530
Bme18I GGWCC 1 cut(s) 526
BmgT120I GGNCC 1 cut(s) 526
BmrFI CCNGG 3 cut(s) 70, 431, 530
BmrI ACTGGG 1 cut(s) 395
BmsI GCATC 1 cut(s) 163
BmtI GCTAGC 1 cut(s) 366
BmuI ACTGGG 1 cut(s) 395
BpmI CTGGAG 1 cut(s) 452
Bpu1102I GCTNAGC 1 cut(s) 498
Bpu14I TTCGAA 2 cut(s) 31, 327
BsaAI YACGTR 1 cut(s) 20
BsaJI CCNNGG 2 cut(s) 69, 121
Bse1I ACTGG 3 cut(s) 293, 390, 480
BseBI CCWGG 3 cut(s) 70, 431, 530
BseDI CCNNGG 2 cut(s) 69, 121
BseMII CTCAG 1 cut(s) 489
BseNI ACTGG 3 cut(s) 293, 390, 480
BseSI GKGCMC 3 cut(s) 19, 25, 440
BseXI GCAGC 2 cut(s) 160, 514
BseYI CCCAGC 1 cut(s) 39
BshFI GGCC 1 cut(s) 323
BsiHKAI GWGCWC 3 cut(s) 19, 25, 440
BslFI GGGAC 1 cut(s) 157
BsmFI GGGAC 1 cut(s) 157
BsmI GAATGC 2 cut(s) 39, 514
BsnI GGCC 1 cut(s) 323
Bsp119I TTCGAA 2 cut(s) 31, 327
Bsp1286I GDGCHC 3 cut(s) 19, 25, 440
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 1 cut(s) 401
Bsp1720I GCTNAGC 1 cut(s) 498
BspACI CCGC 1 cut(s) 495
BspANI GGCC 1 cut(s) 323
BspCNI CTCAG 1 cut(s) 490
BspHI TCATGA 1 cut(s) 306
BspOI GCTAGC 1 cut(s) 366
BspPI GGATC 1 cut(s) 409
BspT104I TTCGAA 2 cut(s) 31, 327
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 3 cut(s) 293, 390, 480
BssECI CCNNGG 2 cut(s) 69, 121
BssMI GATC 1 cut(s) 401
BssT1I CCWWGG 1 cut(s) 121
Bst2UI CCWGG 3 cut(s) 70, 431, 530
Bst4CI ACNGT 4 cut(s) 10, 118, 196, 418
BstAUI TGTACA 1 cut(s) 3
BstBAI YACGTR 1 cut(s) 20
BstBI TTCGAA 2 cut(s) 31, 327
BstC8I GCNNGC 3 cut(s) 58, 364, 440
BstDEI CTNAG 1 cut(s) 498
BstKTI GATC 1 cut(s) 404
BstMBI GATC 1 cut(s) 401
BstNI CCWGG 3 cut(s) 70, 431, 530
BstSCI CCNGG 3 cut(s) 68, 429, 528
BstSLI GKGCMC 3 cut(s) 19, 25, 440
BstV1I GCAGC 2 cut(s) 160, 514
BsuRI GGCC 1 cut(s) 323
BtsIMutI CAGTG 2 cut(s) 6, 192
Cac8I GCNNGC 3 cut(s) 58, 364, 440
CciI TCATGA 1 cut(s) 306
Cfr13I GGNCC 1 cut(s) 526
CseI GACGC 1 cut(s) 154
Csp6I GTAC 2 cut(s) 4, 383
CviAII CATG 3 cut(s) 257, 307, 412
CviJI RGCY 7 cut(s) 43, 60, 151, 323, 458, 464, 505
CviKI_1 RGCY 7 cut(s) 43, 60, 151, 323, 458, 464, 505
CviQI GTAC 2 cut(s) 4, 383
DdeI CTNAG 1 cut(s) 498
DpnI GATC 1 cut(s) 403
DpnII GATC 1 cut(s) 401
DrdI GACNNNNNNGTC 1 cut(s) 165
DseDI GACNNNNNNGTC 1 cut(s) 165
Eco130I CCWWGG 1 cut(s) 121
Eco47I GGWCC 1 cut(s) 526
Eco72I CACGTG 1 cut(s) 20
EcoRII CCWGG 3 cut(s) 68, 429, 528
EcoT14I CCWWGG 1 cut(s) 121
ErhI CCWWGG 1 cut(s) 121
FaeI CATG 3 cut(s) 260, 310, 415
FaiI YATR 8 cut(s) 141, 258, 308, 338, 379, 413, 486, 518
FaqI GGGAC 1 cut(s) 157
FatI CATG 3 cut(s) 256, 306, 411
Fnu4HI GCNGC 2 cut(s) 149, 503
Fsp4HI GCNGC 2 cut(s) 149, 503
FspBI CTAG 3 cut(s) 155, 165, 363
GluI GCNGC 2 cut(s) 149, 503
GsaI CCCAGC 1 cut(s) 43
GsuI CTGGAG 1 cut(s) 452
HaeIII GGCC 1 cut(s) 323
HgaI GACGC 1 cut(s) 154
Hin1II CATG 3 cut(s) 260, 310, 415
HindIII AAGCTT 1 cut(s) 58
HinfI GANTC 2 cut(s) 303, 489
HphI GGTGA 2 cut(s) 58, 238
Hpy166II GTNNAC 4 cut(s) 6, 17, 23, 438
Hpy188I TCNGA 2 cut(s) 99, 246
Hpy188III TCNNGA 3 cut(s) 170, 188, 307
Hpy8I GTNNAC 4 cut(s) 6, 17, 23, 438
HpyAV CCTTC 3 cut(s) 43, 334, 464
HpyCH4III ACNGT 4 cut(s) 10, 118, 196, 418
HpyCH4IV ACGT 1 cut(s) 19
HpyCH4V TGCA 8 cut(s) 17, 23, 56, 176, 222, 315, 422, 438
HpyF3I CTNAG 1 cut(s) 498
HpySE526I ACGT 1 cut(s) 19
Hsp92II CATG 3 cut(s) 260, 310, 415
Kzo9I GATC 1 cut(s) 401
Lsp1109I GCAGC 2 cut(s) 160, 514
LweI GCATC 1 cut(s) 163
MaeI CTAG 3 cut(s) 155, 165, 363
MaeII ACGT 1 cut(s) 19
MalI GATC 1 cut(s) 403
MboI GATC 1 cut(s) 401
MfeI CAATTG 1 cut(s) 177
MhlI GDGCHC 3 cut(s) 19, 25, 440
MluCI AATT 3 cut(s) 133, 177, 480
MmeI TCCRAC 1 cut(s) 427
MnlI CCTC 1 cut(s) 75
MseI TTAA 1 cut(s) 231
MspA1I CMGCKG 2 cut(s) 497, 505
MspR9I CCNGG 3 cut(s) 70, 431, 530
MunI CAATTG 1 cut(s) 177
Mva1269I GAATGC 2 cut(s) 39, 514
MvaI CCWGG 3 cut(s) 70, 431, 530
NdeII GATC 1 cut(s) 401
NheI GCTAGC 1 cut(s) 362
NlaIII CATG 3 cut(s) 260, 310, 415
NspV TTCGAA 2 cut(s) 31, 327
PagI TCATGA 1 cut(s) 306
PctI GAATGC 2 cut(s) 39, 514
PfeI GAWTC 2 cut(s) 303, 489
PfoI TCCNGGA 1 cut(s) 429
PkrI GCNGC 2 cut(s) 150, 504
PmaCI CACGTG 1 cut(s) 20
PmlI CACGTG 1 cut(s) 20
Ppu21I YACGTR 1 cut(s) 20
PshBI ATTAAT 1 cut(s) 231
Psp6I CCWGG 3 cut(s) 68, 429, 528
PspCI CACGTG 1 cut(s) 20
PspFI CCCAGC 1 cut(s) 39
PspGI CCWGG 3 cut(s) 68, 429, 528
PspPI GGNCC 1 cut(s) 526
PvuII CAGCTG 1 cut(s) 505
RsaI GTAC 2 cut(s) 5, 384
RsaNI GTAC 2 cut(s) 4, 383
SaqAI TTAA 1 cut(s) 231
SatI GCNGC 2 cut(s) 149, 503
Sau3AI GATC 1 cut(s) 401
Sau96I GGNCC 1 cut(s) 526
ScrFI CCNGG 3 cut(s) 70, 431, 530
SduI GDGCHC 3 cut(s) 19, 25, 440
SetI ASST 7 cut(s) 22, 45, 62, 153, 228, 507, 534
SfaNI GCATC 1 cut(s) 163
SfuI TTCGAA 2 cut(s) 31, 327
SinI GGWCC 1 cut(s) 526
SpeI ACTAGT 1 cut(s) 164
Sse9I AATT 3 cut(s) 133, 177, 480
SsiI CCGC 1 cut(s) 495
SspMI CTAG 3 cut(s) 155, 165, 363
StyD4I CCNGG 3 cut(s) 68, 429, 528
StyI CCWWGG 1 cut(s) 121
TaaI ACNGT 4 cut(s) 10, 118, 196, 418
TaiI ACGT 1 cut(s) 22
TaqI TCGA 2 cut(s) 31, 327
TasI AATT 3 cut(s) 133, 177, 480
TatI WGTACW 1 cut(s) 3
TfiI GAWTC 2 cut(s) 303, 489
Tru1I TTAA 1 cut(s) 231
Tru9I TTAA 1 cut(s) 231
TscAI CASTG 2 cut(s) 13, 199
TseI GCWGC 2 cut(s) 148, 502
TspDTI ATGAA 6 cut(s) 52, 263, 273, 295, 473, 533
TspRI CASTG 2 cut(s) 13, 199
VneI GTGCAC 3 cut(s) 15, 21, 436
VpaK11BI GGWCC 1 cut(s) 526
VspI ATTAAT 1 cut(s) 231
XapI RAATTY 1 cut(s) 480
XcmI CCANNNNNNNNNTGG 1 cut(s) 472
XspI CTAG 3 cut(s) 155, 165, 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.