Rh1DG033100

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0005549
Physical Location & Seq
Reverse (-)
4875555 .. 4878034
2480 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG033100.1

Sequence Viewer

Length: 822 bp
ATGGCTATAGTAGAATTTGGCATGCTTCTGGGAGGTGATTTTAAATCTTTTAGTGAGGAGTATAAGATAGTTATGGTCGAACCATTCAATTCAATCAGAAAAAAGATGTCGGTTCAAGTGGCTCTTCCTGGTAGTGATATGTGTCGAGCATTCTGCAAAGGAGCATCAGAAATTATTTTAGGAATGTGTGACAAGGTTGTCAATACTGAAGGATCAGCTCCTCTCACTGCTGTCCAGATGCTTTGGGTAAACTTGATTATGGATACTCTTGGCGCTTTGGCACTGGCTACAGAGCCACCGCATGATGGCCTTATGAAAAGACCACCAGTTGGAAGAAATATTAAATTCATCACTGGGATCATGTGGAGGAATATTATCGGTCAGAGTATCTATCAAATTGCTGTCCTTCTGGTTCTTAATTTTTGTGGGAAACGTCTTTTAAAACTCACCTGTCCAAATGCAAGTTCCATTCTCAAGACCTTCATATTCAACTGTTTTGTATTTTGCCAGGTATTCAACGAGATTAACAGCCGTGATATGGAGAAAATCAATGTGTTCAGAGGGATGTTTAATAGTTATACTTTCATGATAGTGATGATCTGCACAGTAGTTTTCCAGATCATAATAGTTGAATTTTTGGGCACTTTTGCTCAAACTGTGCCACTGAGCTGGAAGTTGTGGTTAGCAAGCGTCTTGATTGGAGCCGTAAGTTTACCGGTTGCTGTTGTCTTGAAGTGCATTCCTGTATCATTAAGGAAACAAGCTGCTAAGGATCACAGTGATATCCACGAACCTCTTCTCCGTGGCCCTGAGCTGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009705 GO:0010035 GO:0010941 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0042742 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055081 GO:0055085 GO:0060548 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098542 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase Hydrolase_3

Protein Analysis

273

Amino Acids

30.35

Weight (kDa)

8.85

Isoelectric Point (pI)

37.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_ATPase PF13246 2 - 80 3.6e-11 P-type ATPase, cytoplasmic domain N
Cation_ATPase_C PF00689 73 - 246 1.2e-41 Cation transporting ATPase, C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 197
AccB7I CCANNNNNTGG 1 cut(s) 329
AciI CCGC 1 cut(s) 299
AclWI GGATC 3 cut(s) 220, 365, 780
AcsI RAATTY 3 cut(s) 14, 344, 632
AcuI CTGAAG 1 cut(s) 228
AfiI CCNNNNNNNGG 4 cut(s) 305, 329, 538, 814
AgeI ACCGGT 1 cut(s) 715
AgsI TTSAA 7 cut(s) 88, 93, 116, 490, 517, 632, 733
AjnI CCWGG 2 cut(s) 127, 507
AjuI GAANNNNNNNTTGG 2 cut(s) 448, 480
AloI GAACNNNNNNTCC 2 cut(s) 783, 815
AluBI AGCT 4 cut(s) 218, 669, 764, 814
AluI AGCT 4 cut(s) 218, 669, 764, 814
AlwI GGATC 3 cut(s) 220, 365, 780
AoxI GGCC 2 cut(s) 307, 805
ApeKI GCWGC 1 cut(s) 764
ApoI RAATTY 3 cut(s) 14, 344, 632
AsiGI ACCGGT 1 cut(s) 715
Asp700I GAANNNNTTC 1 cut(s) 795
AspLEI GCGC 1 cut(s) 275
AspS9I GGNCC 1 cut(s) 806
AsuHPI GGTGA 2 cut(s) 47, 439
BaeGI GKGCMC 1 cut(s) 644
BbvI GCAGC 1 cut(s) 751
BccI CCATC 1 cut(s) 299
BceAI ACGGC 2 cut(s) 516, 689
BcgI CGANNNNNNTGC 2 cut(s) 135, 169
BciT130I CCWGG 2 cut(s) 129, 509
BciVI GTATCC 1 cut(s) 256
BfmI CTRYAG 2 cut(s) 6, 288
BfoI RGCGCY 1 cut(s) 276
BfuI GTATCC 1 cut(s) 256
BisI GCNGC 1 cut(s) 765
BlsI GCNGC 1 cut(s) 766
Bme1390I CCNGG 2 cut(s) 129, 509
BmgT120I GGNCC 1 cut(s) 806
BmiI GGNNCC 1 cut(s) 703
BmrFI CCNGG 2 cut(s) 129, 509
BmrI ACTGGG 1 cut(s) 363
BmsI GCATC 2 cut(s) 173, 228
BmuI ACTGGG 1 cut(s) 363
Bpu10I CCTNAGC 2 cut(s) 768, 810
BpuEI CTTGAG 1 cut(s) 458
BsaJI CCNNGG 1 cut(s) 802
BsaWI WCCGGW 1 cut(s) 715
BsaXI ACNNNNNCTCC 2 cut(s) 783, 813
Bsc4I CCNNNNNNNGG 4 cut(s) 305, 329, 538, 814
Bse118I RCCGGY 1 cut(s) 715
Bse1I ACTGG 3 cut(s) 288, 326, 358
BseBI CCWGG 2 cut(s) 129, 509
BseDI CCNNGG 1 cut(s) 802
BseGI GGATG 1 cut(s) 570
BseLI CCNNNNNNNGG 4 cut(s) 305, 329, 538, 814
BseMII CTCAG 2 cut(s) 656, 801
BseNI ACTGG 3 cut(s) 288, 326, 358
BseRI GAGGAG 2 cut(s) 71, 210
BseSI GKGCMC 1 cut(s) 644
BseXI GCAGC 1 cut(s) 751
BsgI GTGCAG 1 cut(s) 586
BshFI GGCC 2 cut(s) 309, 807
BshTI ACCGGT 1 cut(s) 715
BsiSI CCGG 1 cut(s) 716
BslI CCNNNNNNNGG 4 cut(s) 305, 329, 538, 814
BsmI GAATGC 2 cut(s) 149, 738
BsnI GGCC 2 cut(s) 309, 807
Bsp1286I GDGCHC 1 cut(s) 644
Bsp143I GATC 5 cut(s) 212, 357, 597, 618, 772
BspACI CCGC 1 cut(s) 299
BspANI GGCC 2 cut(s) 309, 807
BspCNI CTCAG 2 cut(s) 657, 802
BspHI TCATGA 1 cut(s) 585
BspLI GGNNCC 1 cut(s) 703
BspPI GGATC 3 cut(s) 220, 365, 780
BspQI GCTCTTC 1 cut(s) 129
BsrFI RCCGGY 1 cut(s) 715
BsrI ACTGG 3 cut(s) 288, 326, 358
BssAI RCCGGY 1 cut(s) 715
BssECI CCNNGG 1 cut(s) 802
BssMI GATC 5 cut(s) 212, 357, 597, 618, 772
Bst2UI CCWGG 2 cut(s) 129, 509
Bst4CI ACNGT 4 cut(s) 494, 607, 658, 779
Bst6I CTCTTC 2 cut(s) 129, 801
BstC8I GCNNGC 3 cut(s) 23, 688, 816
BstDEI CTNAG 3 cut(s) 665, 768, 810
BstDSI CCRYGG 1 cut(s) 802
BstF5I GGATG 1 cut(s) 570
BstH2I RGCGCY 1 cut(s) 276
BstHHI GCGC 1 cut(s) 275
BstKTI GATC 5 cut(s) 215, 360, 600, 621, 775
BstMBI GATC 5 cut(s) 212, 357, 597, 618, 772
BstNI CCWGG 2 cut(s) 129, 509
BstNSI RCATGY 1 cut(s) 25
BstSCI CCNGG 2 cut(s) 127, 507
BstSFI CTRYAG 2 cut(s) 6, 288
BstSLI GKGCMC 1 cut(s) 644
BstV1I GCAGC 1 cut(s) 751
BstXI CCANNNNNNTGG 1 cut(s) 669
BsuI GTATCC 1 cut(s) 256
BsuRI GGCC 2 cut(s) 309, 807
BtgI CCRYGG 1 cut(s) 802
BtsCI GGATG 1 cut(s) 570
BtsI GCAGTG 1 cut(s) 225
BtsIMutI CAGTG 5 cut(s) 225, 281, 351, 662, 784
Cac8I GCNNGC 3 cut(s) 23, 688, 816
CciI TCATGA 1 cut(s) 585
CfoI GCGC 1 cut(s) 275
Cfr10I RCCGGY 1 cut(s) 715
Cfr13I GGNCC 1 cut(s) 806
CseI GACGC 1 cut(s) 679
CspAI ACCGGT 1 cut(s) 715
CviAII CATG 4 cut(s) 22, 302, 361, 586
DdeI CTNAG 3 cut(s) 665, 768, 810
DpnI GATC 5 cut(s) 214, 359, 599, 620, 774
DpnII GATC 5 cut(s) 212, 357, 597, 618, 772
DraI TTTAAA 2 cut(s) 43, 441
DrdI GACNNNNNNGTC 1 cut(s) 197
DseDI GACNNNNNNGTC 1 cut(s) 197
Eam1104I CTCTTC 2 cut(s) 129, 801
EarI CTCTTC 2 cut(s) 129, 801
Eco32I GATATC 1 cut(s) 784
Eco57I CTGAAG 1 cut(s) 228
EcoRII CCWGG 2 cut(s) 127, 507
EcoRV GATATC 1 cut(s) 784
FaeI CATG 4 cut(s) 25, 305, 364, 589
FalI AAGNNNNNCTT 2 cut(s) 108, 140
FatI CATG 4 cut(s) 21, 301, 360, 585
Fnu4HI GCNGC 1 cut(s) 765
FokI GGATG 1 cut(s) 577
Fsp4HI GCNGC 1 cut(s) 765
GlaI GCGC 1 cut(s) 274
GluI GCNGC 1 cut(s) 765
HaeII RGCGCY 1 cut(s) 276
HaeIII GGCC 2 cut(s) 309, 807
HapII CCGG 1 cut(s) 716
HgaI GACGC 1 cut(s) 679
HhaI GCGC 1 cut(s) 275
Hin1II CATG 4 cut(s) 25, 305, 364, 589
Hin6I GCGC 1 cut(s) 273
HinP1I GCGC 1 cut(s) 273
HpaII CCGG 1 cut(s) 716
HphI GGTGA 2 cut(s) 47, 439
Hpy166II GTNNAC 2 cut(s) 250, 713
Hpy188I TCNGA 4 cut(s) 98, 169, 384, 560
Hpy188III TCNNGA 6 cut(s) 235, 475, 586, 616, 694, 730
Hpy8I GTNNAC 2 cut(s) 250, 713
HpyAV CCTTC 3 cut(s) 203, 416, 490
HpyCH4III ACNGT 4 cut(s) 494, 607, 658, 779
HpyCH4IV ACGT 1 cut(s) 433
HpyCH4V TGCA 4 cut(s) 156, 461, 603, 738
HpyF3I CTNAG 3 cut(s) 665, 768, 810
HpySE526I ACGT 1 cut(s) 433
Hsp92II CATG 4 cut(s) 25, 305, 364, 589
HspAI GCGC 1 cut(s) 273
Kzo9I GATC 5 cut(s) 212, 357, 597, 618, 772
LguI GCTCTTC 1 cut(s) 129
LmnI GCTCC 3 cut(s) 161, 223, 701
Lsp1109I GCAGC 1 cut(s) 751
LweI GCATC 2 cut(s) 173, 228
MaeII ACGT 1 cut(s) 433
MaeIII GTNAC 1 cut(s) 188
MalI GATC 5 cut(s) 214, 359, 599, 620, 774
MboI GATC 5 cut(s) 212, 357, 597, 618, 772
MboII GAAGA 3 cut(s) 116, 345, 788
MhlI GDGCHC 1 cut(s) 644
MluCI AATT 7 cut(s) 14, 88, 171, 344, 396, 418, 632
MmeI TCCRAC 1 cut(s) 310
MnlI CCTC 6 cut(s) 26, 49, 231, 360, 554, 804
MroXI GAANNNNTTC 1 cut(s) 795
MseI TTAA 7 cut(s) 42, 342, 417, 440, 525, 570, 752
MslI CAYNNNNRTG 1 cut(s) 590
MspI CCGG 1 cut(s) 716
MspR9I CCNGG 2 cut(s) 129, 509
Mva1269I GAATGC 2 cut(s) 149, 738
MvaI CCWGG 2 cut(s) 129, 509
NdeII GATC 5 cut(s) 212, 357, 597, 618, 772
NlaIII CATG 4 cut(s) 25, 305, 364, 589
NlaIV GGNNCC 1 cut(s) 703
NmuCI GTSAC 1 cut(s) 188
NspI RCATGY 1 cut(s) 25
PaeI GCATGC 1 cut(s) 25
PagI TCATGA 1 cut(s) 585
PciSI GCTCTTC 1 cut(s) 129
PctI GAATGC 2 cut(s) 149, 738
PdmI GAANNNNTTC 1 cut(s) 795
PflMI CCANNNNNTGG 1 cut(s) 329
PinAI ACCGGT 1 cut(s) 715
PkrI GCNGC 1 cut(s) 766
Psp6I CCWGG 2 cut(s) 127, 507
PspGI CCWGG 2 cut(s) 127, 507
PspN4I GGNNCC 1 cut(s) 703
PspPI GGNCC 1 cut(s) 806
RseI CAYNNNNRTG 1 cut(s) 590
SapI GCTCTTC 1 cut(s) 129
SaqAI TTAA 7 cut(s) 42, 342, 417, 440, 525, 570, 752
SatI GCNGC 1 cut(s) 765
Sau3AI GATC 5 cut(s) 212, 357, 597, 618, 772
Sau96I GGNCC 1 cut(s) 806
ScrFI CCNGG 2 cut(s) 129, 509
SduI GDGCHC 1 cut(s) 644
SfaNI GCATC 2 cut(s) 173, 228
SfcI CTRYAG 2 cut(s) 6, 288
SmiMI CAYNNNNRTG 1 cut(s) 590
SmlI CTYRAG 1 cut(s) 473
SmoI CTYRAG 1 cut(s) 473
SphI GCATGC 1 cut(s) 25
Sse9I AATT 7 cut(s) 14, 88, 171, 344, 396, 418, 632
SsiI CCGC 1 cut(s) 299
SspI AATATT 2 cut(s) 340, 373
StyD4I CCNGG 2 cut(s) 127, 507
TaaI ACNGT 4 cut(s) 494, 607, 658, 779
TaiI ACGT 1 cut(s) 436
TaqI TCGA 2 cut(s) 78, 145
TaqII GACCGA 1 cut(s) 368
TasI AATT 7 cut(s) 14, 88, 171, 344, 396, 418, 632
Tru1I TTAA 7 cut(s) 42, 342, 417, 440, 525, 570, 752
Tru9I TTAA 7 cut(s) 42, 342, 417, 440, 525, 570, 752
TscAI CASTG 5 cut(s) 232, 288, 358, 669, 784
TseFI GTSAC 1 cut(s) 188
TseI GCWGC 1 cut(s) 764
Tsp45I GTSAC 1 cut(s) 188
TspDTI ATGAA 4 cut(s) 329, 337, 472, 574
TspGWI ACGGA 1 cut(s) 791
TspRI CASTG 5 cut(s) 232, 288, 358, 669, 784
Van91I CCANNNNNTGG 1 cut(s) 329
XapI RAATTY 3 cut(s) 14, 344, 632
XceI RCATGY 1 cut(s) 25
XmnI GAANNNNTTC 1 cut(s) 795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.