Rmu_sc0006349.1_g000007

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006349.1
OG0005549
Physical Location & Seq
Reverse (-)
27597 .. 30181
2585 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006349.1_g000007.1.cds

Sequence Viewer

Length: 894 bp
atgactccgacgtccagcaagtcagcaagtggactcatcaagtcagcaattcatgaaaagaaactgacgaattacttgactgattgttttgatcaattcggaaagcgtaaggccatagatgatcaggtgcacaaacaattaactgcagtaagtgtggccagatggagtcgtccatacataaacatccgaaagtttatgcggttccagttagcaattaacgttgttgctctggtgatcaatgctgtttctgtacatgtttcaggaaacgttccccttactgctgtgcaattgctttgggtcaacctgattatgtccactgttggtgcacgtgcacttatacgaatgccccagtttttgaagggtgcaagcttcatcaccaggggcatttggaggaatatcattggtcagagtatctatcaaccgactgtccttggatttctcaatttctatgggacgcagctactaggacttactagtccagatgcaattgctgttcttgacactgtgatattcaactcaatagtgttttgcaaggcaactttaactggattcatgatttgcactttggccttcgaagttgtcatagttgagttcttgggagtggttgctagcattgtgccaatatgggtactgggcatttttattggatcagttagcatgactgttgcagttgtcctggagtgcacgcctgttggaagttcagccaaagggactggcgatggcttggccacagaaggactggaaattcatagattctccgctgagcagctgaatgctgttatgaagttggtccaggaaaaattccaagtcgcttttcatggtccaaaaacagcactactgaagatcaattacagtgccattagatctgttggtgaagttagaatctcagcttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009624 GO:0009628 GO:0009705 GO:0010035 GO:0010941 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0042742 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055081 GO:0055085 GO:0060548 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098542 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

297

Amino Acids

32.31

Weight (kDa)

9.69

Isoelectric Point (pI)

28.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 10, 474
AatII GACGTC 1 cut(s) 14
AciI CCGC 2 cut(s) 199, 759
AclI AACGTT 2 cut(s) 219, 267
AclWI GGATC 1 cut(s) 655
AcoI YGGCCR 2 cut(s) 156, 726
AcsI RAATTY 2 cut(s) 744, 800
AcuI CTGAAG 1 cut(s) 860
AcvI CACGTG 1 cut(s) 329
AcyI GRCGYC 1 cut(s) 11
AfaI GTAC 2 cut(s) 252, 630
AflIII ACRYGT 1 cut(s) 253
AgsI TTSAA 2 cut(s) 358, 514
AhlI ACTAGT 1 cut(s) 473
AjnI CCWGG 3 cut(s) 377, 675, 792
AjuI GAANNNNNNNTTGG 2 cut(s) 798, 830
AluBI AGCT 4 cut(s) 369, 460, 769, 890
AluI AGCT 4 cut(s) 369, 460, 769, 890
Alw21I GWGCWC 4 cut(s) 132, 328, 334, 686
Alw44I GTGCAC 4 cut(s) 128, 324, 330, 682
AlwI GGATC 1 cut(s) 655
AoxI GGCC 4 cut(s) 111, 156, 567, 726
ApaLI GTGCAC 4 cut(s) 128, 324, 330, 682
ApeKI GCWGC 2 cut(s) 457, 766
ApoI RAATTY 2 cut(s) 744, 800
AspS9I GGNCC 2 cut(s) 790, 821
AsuHPI GGTGA 3 cut(s) 244, 367, 884
AsuII TTCGAA 1 cut(s) 573
AsuNHI GCTAGC 1 cut(s) 608
AvaII GGWCC 2 cut(s) 790, 821
BaeGI GKGCMC 4 cut(s) 132, 328, 334, 686
BalI TGGCCA 2 cut(s) 158, 728
BarI GAAGNNNNNNTAC 2 cut(s) 833, 865
BbrPI CACGTG 1 cut(s) 329
Bbv12I GWGCWC 4 cut(s) 132, 328, 334, 686
BbvI GCAGC 2 cut(s) 469, 778
BccI CCATC 2 cut(s) 156, 713
BciT130I CCWGG 3 cut(s) 379, 677, 794
BclI TGATCA 3 cut(s) 91, 121, 234
BcuI ACTAGT 1 cut(s) 473
BfaI CTAG 3 cut(s) 464, 474, 609
BfmI CTRYAG 1 cut(s) 144
BglII AGATCT 1 cut(s) 863
BisI GCNGC 2 cut(s) 458, 767
BlpI GCTNAGC 1 cut(s) 762
BlsI GCNGC 2 cut(s) 459, 768
Bme1390I CCNGG 3 cut(s) 379, 677, 794
Bme18I GGWCC 2 cut(s) 790, 821
BmgT120I GGNCC 2 cut(s) 790, 821
BmiI GGNNCC 1 cut(s) 203
BmrFI CCNGG 3 cut(s) 379, 677, 794
BmrI ACTGGG 2 cut(s) 343, 641
BmsI GCATC 1 cut(s) 472
BmtI GCTAGC 1 cut(s) 612
BmuI ACTGGG 2 cut(s) 343, 641
BpmI CTGGAG 1 cut(s) 698
Bpu1102I GCTNAGC 1 cut(s) 762
Bpu14I TTCGAA 1 cut(s) 573
BsaAI YACGTR 1 cut(s) 329
BsaHI GRCGYC 1 cut(s) 11
BsaJI CCNNGG 2 cut(s) 378, 430
Bse1I ACTGG 6 cut(s) 205, 349, 550, 636, 718, 744
BseBI CCWGG 3 cut(s) 379, 677, 794
BseDI CCNNGG 2 cut(s) 378, 430
BseGI GGATG 1 cut(s) 183
BseMII CTCAG 1 cut(s) 753
BseNI ACTGG 6 cut(s) 205, 349, 550, 636, 718, 744
BseSI GKGCMC 4 cut(s) 132, 328, 334, 686
BseXI GCAGC 2 cut(s) 469, 778
BshFI GGCC 4 cut(s) 113, 158, 569, 728
BsiHKAI GWGCWC 4 cut(s) 132, 328, 334, 686
BslFI GGGAC 2 cut(s) 466, 724
BsmFI GGGAC 2 cut(s) 466, 724
BsmI GAATGC 2 cut(s) 348, 778
BsnI GGCC 4 cut(s) 113, 158, 569, 728
Bsp119I TTCGAA 1 cut(s) 573
Bsp1286I GDGCHC 4 cut(s) 132, 328, 334, 686
Bsp1407I TGTACA 1 cut(s) 250
Bsp143I GATC 6 cut(s) 91, 121, 234, 647, 843, 863
Bsp1720I GCTNAGC 1 cut(s) 762
BspACI CCGC 2 cut(s) 199, 759
BspANI GGCC 4 cut(s) 113, 158, 569, 728
BspCNI CTCAG 1 cut(s) 754
BspHI TCATGA 2 cut(s) 52, 552
BspLI GGNNCC 1 cut(s) 203
BspMAI CTGCAG 1 cut(s) 148
BspOI GCTAGC 1 cut(s) 612
BspPI GGATC 1 cut(s) 655
BspT104I TTCGAA 1 cut(s) 573
BsrGI TGTACA 1 cut(s) 250
BsrI ACTGG 6 cut(s) 205, 349, 550, 636, 718, 744
BssECI CCNNGG 2 cut(s) 378, 430
BssMI GATC 6 cut(s) 91, 121, 234, 647, 843, 863
BssNI GRCGYC 1 cut(s) 11
BssT1I CCWWGG 1 cut(s) 430
Bst2UI CCWGG 3 cut(s) 379, 677, 794
Bst4CI ACNGT 5 cut(s) 319, 427, 505, 664, 854
BstACI GRCGYC 1 cut(s) 11
BstAUI TGTACA 1 cut(s) 250
BstBAI YACGTR 1 cut(s) 329
BstBI TTCGAA 1 cut(s) 573
BstC8I GCNNGC 3 cut(s) 367, 610, 686
BstDEI CTNAG 2 cut(s) 762, 886
BstF5I GGATG 1 cut(s) 183
BstKTI GATC 6 cut(s) 94, 124, 237, 650, 846, 866
BstMBI GATC 6 cut(s) 91, 121, 234, 647, 843, 863
BstNI CCWGG 3 cut(s) 379, 677, 794
BstNSI RCATGY 1 cut(s) 257
BstSCI CCNGG 3 cut(s) 377, 675, 792
BstSFI CTRYAG 1 cut(s) 144
BstSLI GKGCMC 4 cut(s) 132, 328, 334, 686
BstV1I GCAGC 2 cut(s) 469, 778
BstX2I RGATCY 1 cut(s) 863
BstYI RGATCY 1 cut(s) 863
BsuRI GGCC 4 cut(s) 113, 158, 569, 728
BtgZI GCGATG 1 cut(s) 732
BtsCI GGATG 1 cut(s) 183
BtsIMutI CAGTG 3 cut(s) 315, 501, 859
Cac8I GCNNGC 3 cut(s) 367, 610, 686
CciI TCATGA 2 cut(s) 52, 552
Cfr13I GGNCC 2 cut(s) 790, 821
CseI GACGC 1 cut(s) 463
Csp6I GTAC 2 cut(s) 251, 629
CviAII CATG 5 cut(s) 53, 254, 553, 658, 818
CviQI GTAC 2 cut(s) 251, 629
DdeI CTNAG 2 cut(s) 762, 886
DpnI GATC 6 cut(s) 93, 123, 236, 649, 845, 865
DpnII GATC 6 cut(s) 91, 121, 234, 647, 843, 863
DrdI GACNNNNNNGTC 2 cut(s) 10, 474
DseDI GACNNNNNNGTC 2 cut(s) 10, 474
EaeI YGGCCR 2 cut(s) 156, 726
Eco130I CCWWGG 1 cut(s) 430
Eco47I GGWCC 2 cut(s) 790, 821
Eco57I CTGAAG 1 cut(s) 860
Eco72I CACGTG 1 cut(s) 329
EcoRII CCWGG 3 cut(s) 377, 675, 792
EcoT14I CCWWGG 1 cut(s) 430
ErhI CCWWGG 1 cut(s) 430
FaeI CATG 5 cut(s) 56, 257, 556, 661, 821
FaqI GGGAC 2 cut(s) 466, 724
FatI CATG 5 cut(s) 52, 253, 552, 657, 817
FbaI TGATCA 3 cut(s) 91, 121, 234
Fnu4HI GCNGC 2 cut(s) 458, 767
FokI GGATG 1 cut(s) 170
Fsp4HI GCNGC 2 cut(s) 458, 767
FspBI CTAG 3 cut(s) 464, 474, 609
GluI GCNGC 2 cut(s) 458, 767
GsuI CTGGAG 1 cut(s) 698
HaeIII GGCC 4 cut(s) 113, 158, 569, 728
HgaI GACGC 1 cut(s) 463
Hin1I GRCGYC 1 cut(s) 11
Hin1II CATG 5 cut(s) 56, 257, 556, 661, 821
HincII GTYRAC 1 cut(s) 301
HindII GTYRAC 1 cut(s) 301
HindIII AAGCTT 1 cut(s) 367
HinfI GANTC 6 cut(s) 4, 33, 166, 549, 753, 882
HphI GGTGA 3 cut(s) 244, 367, 884
Hpy166II GTNNAC 7 cut(s) 32, 130, 301, 315, 326, 332, 684
Hpy188I TCNGA 4 cut(s) 9, 101, 188, 408
Hpy188III TCNNGA 5 cut(s) 53, 261, 479, 497, 553
Hpy8I GTNNAC 7 cut(s) 32, 130, 301, 315, 326, 332, 684
Hpy99I CGWCG 1 cut(s) 13
HpyAV CCTTC 3 cut(s) 352, 580, 728
HpyCH4III ACNGT 5 cut(s) 319, 427, 505, 664, 854
HpyCH4IV ACGT 4 cut(s) 11, 219, 267, 328
HpyF3I CTNAG 2 cut(s) 762, 886
HpySE526I ACGT 4 cut(s) 11, 219, 267, 328
Hsp92I GRCGYC 1 cut(s) 11
Hsp92II CATG 5 cut(s) 56, 257, 556, 661, 821
Ksp22I TGATCA 3 cut(s) 91, 121, 234
Kzo9I GATC 6 cut(s) 91, 121, 234, 647, 843, 863
Lsp1109I GCAGC 2 cut(s) 469, 778
LweI GCATC 1 cut(s) 472
MaeI CTAG 3 cut(s) 464, 474, 609
MaeII ACGT 4 cut(s) 11, 219, 267, 328
MalI GATC 6 cut(s) 93, 123, 236, 649, 845, 865
MboI GATC 6 cut(s) 91, 121, 234, 647, 843, 863
MboII GAAGA 1 cut(s) 853
MfeI CAATTG 2 cut(s) 287, 486
MflI RGATCY 1 cut(s) 863
MhlI GDGCHC 4 cut(s) 132, 328, 334, 686
MlsI TGGCCA 2 cut(s) 158, 728
MluNI TGGCCA 2 cut(s) 158, 728
MlyI GAGTC 2 cut(s) 27, 175
MmeI TCCRAC 2 cut(s) 32, 673
MnlI CCTC 1 cut(s) 384
Mox20I TGGCCA 2 cut(s) 158, 728
MscI TGGCCA 2 cut(s) 158, 728
MseI TTAA 4 cut(s) 140, 216, 542, 892
Msp20I TGGCCA 2 cut(s) 158, 728
MspA1I CMGCKG 2 cut(s) 761, 769
MspR9I CCNGG 3 cut(s) 379, 677, 794
MunI CAATTG 2 cut(s) 287, 486
Mva1269I GAATGC 2 cut(s) 348, 778
MvaI CCWGG 3 cut(s) 379, 677, 794
NdeII GATC 6 cut(s) 91, 121, 234, 647, 843, 863
NheI GCTAGC 1 cut(s) 608
NlaIII CATG 5 cut(s) 56, 257, 556, 661, 821
NlaIV GGNNCC 1 cut(s) 203
NspI RCATGY 1 cut(s) 257
NspV TTCGAA 1 cut(s) 573
PagI TCATGA 2 cut(s) 52, 552
PciI ACATGT 1 cut(s) 253
PctI GAATGC 2 cut(s) 348, 778
PfeI GAWTC 3 cut(s) 549, 753, 882
PfoI TCCNGGA 2 cut(s) 675, 792
PkrI GCNGC 2 cut(s) 459, 768
PleI GAGTC 2 cut(s) 27, 174
PmaCI CACGTG 1 cut(s) 329
PmlI CACGTG 1 cut(s) 329
PpsI GAGTC 2 cut(s) 27, 174
Ppu21I YACGTR 1 cut(s) 329
PscI ACATGT 1 cut(s) 253
Psp1406I AACGTT 2 cut(s) 219, 267
Psp6I CCWGG 3 cut(s) 377, 675, 792
PspCI CACGTG 1 cut(s) 329
PspGI CCWGG 3 cut(s) 377, 675, 792
PspN4I GGNNCC 1 cut(s) 203
PspPI GGNCC 2 cut(s) 790, 821
PstI CTGCAG 1 cut(s) 148
PsuI RGATCY 1 cut(s) 863
PvuII CAGCTG 1 cut(s) 769
RsaI GTAC 2 cut(s) 252, 630
RsaNI GTAC 2 cut(s) 251, 629
SaqAI TTAA 4 cut(s) 140, 216, 542, 892
SatI GCNGC 2 cut(s) 458, 767
Sau3AI GATC 6 cut(s) 91, 121, 234, 647, 843, 863
Sau96I GGNCC 2 cut(s) 790, 821
SchI GAGTC 2 cut(s) 27, 175
ScrFI CCNGG 3 cut(s) 379, 677, 794
SduI GDGCHC 4 cut(s) 132, 328, 334, 686
SfaNI GCATC 1 cut(s) 472
SfcI CTRYAG 1 cut(s) 144
SfuI TTCGAA 1 cut(s) 573
SinI GGWCC 2 cut(s) 790, 821
SpeI ACTAGT 1 cut(s) 473
SsiI CCGC 2 cut(s) 199, 759
SspMI CTAG 3 cut(s) 464, 474, 609
StyD4I CCNGG 3 cut(s) 377, 675, 792
StyI CCWWGG 1 cut(s) 430
TaaI ACNGT 5 cut(s) 319, 427, 505, 664, 854
TaiI ACGT 4 cut(s) 14, 222, 270, 331
TaqI TCGA 1 cut(s) 573
TatI WGTACW 1 cut(s) 250
TfiI GAWTC 3 cut(s) 549, 753, 882
Tru1I TTAA 4 cut(s) 140, 216, 542, 892
Tru9I TTAA 4 cut(s) 140, 216, 542, 892
TscAI CASTG 3 cut(s) 322, 508, 859
TseI GCWGC 2 cut(s) 457, 766
TspDTI ATGAA 7 cut(s) 41, 69, 361, 541, 737, 797, 806
TspRI CASTG 3 cut(s) 322, 508, 859
VneI GTGCAC 4 cut(s) 128, 324, 330, 682
VpaK11BI GGWCC 2 cut(s) 790, 821
XapI RAATTY 2 cut(s) 744, 800
XceI RCATGY 1 cut(s) 257
XcmI CCANNNNNNNNNTGG 1 cut(s) 736
XspI CTAG 3 cut(s) 464, 474, 609
ZraI GACGTC 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.