pycom14g06560

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0005549
Physical Location & Seq
Forward (+)
6503587 .. 6505317
1731 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g06560.1

Sequence Viewer

Length: 867 bp
ATGACTGGTGATGGGACTAACGACGCTCATGCGTTGAAAGAGTCAGACATTGGACTTGCTGTGGGCATAGTAGGAACGGAGGCTGCCAAAGAGAGTGCCGATGTCATCATATTGGATGACAATTTTAAAACTATGGTAAATGTGGCCAGATGGGGACGTTCATCCTCATTTCTTTCAGGATCTTCTCCCCTCACAGCGGTGCAACTGCTTTGGGTGAACATGATTATGGACGCTCTTGGTGCTTCGGCACTGGCAACAGAGCCTCCAAATGATGGACTTATGGAAAGGCCCCCAGTTGGGAGGGGTACAAGCTTCATCACCAAGGCTATGTGGAGGAATATCATTGGTCAGAGTATCTATCAACTGGCTGTCCTTGGAGTTCTCAATTTGTCTGGGAAGAAGCTAATAGGACTTGCTGGTTCAGATGCAACTGAGGTTCTTAACACTGTCATATTCAACGCATTCGTGTTTTGCCAGGTGTTTAATCAGATAAACAGTCGTGACATAGAGAAGATAAACATATTCCGCGGAATGTTTGACAGCTGGATATTCCTAGGCGTCATGGTTTGCACAGCGGCTTTCCAGGTTATCATAGTAGAGTTTTTGGGAGCTTTTGCTTGTACTGTTCCACTAAGCTGGCAACTATGGCTACTCACCATTTTACTTGGATCAGTTAGCATGCTTGTTGCAGTTGTGCTCAAATTAATACCTGTCGAAAGGACAATTAAGCACCATGAAGGATATGAAGCACTTCCTTCTGGTGCTCCAGAGGGCATAGTTTGCATAGGACCGTGTCAAACGCCCTTTTTTCCTTGGAACTTCCTGAGGTGGACGACTGGTACTGCTGAATCTAGCGCAACGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009624 GO:0009628 GO:0009705 GO:0010035 GO:0010941 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0042742 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055081 GO:0055085 GO:0060548 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098542 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

289

Amino Acids

31.06

Weight (kDa)

5.09

Isoelectric Point (pI)

34.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_ATPase_C PF00689 62 - 236 2.9e-42 Cation transporting ATPase, C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 272
AccII CGCG 1 cut(s) 528
AciI CCGC 4 cut(s) 197, 526, 528, 575
AclWI GGATC 2 cut(s) 187, 676
AcoI YGGCCR 1 cut(s) 144
AcyI GRCGYC 1 cut(s) 558
AfaI GTAC 3 cut(s) 307, 622, 841
AfiI CCNNNNNNNGG 4 cut(s) 196, 272, 296, 297
AgsI TTSAA 2 cut(s) 37, 457
AjnI CCWGG 2 cut(s) 474, 582
AleI CACNNNNGTG 1 cut(s) 197
AluBI AGCT 5 cut(s) 312, 403, 543, 611, 636
AluI AGCT 5 cut(s) 312, 403, 543, 611, 636
Alw21I GWGCWC 2 cut(s) 699, 766
AlwI GGATC 2 cut(s) 187, 676
AoxI GGCC 2 cut(s) 144, 287
ApeKI GCWGC 1 cut(s) 83
AseI ATTAAT 1 cut(s) 704
Asp700I GAANNNNTTC 1 cut(s) 750
AspA2I CCTAGG 1 cut(s) 553
AspLEI GCGC 1 cut(s) 857
AspS9I GGNCC 2 cut(s) 288, 788
AsuHPI GGTGA 4 cut(s) 20, 226, 310, 646
AvaII GGWCC 1 cut(s) 788
AvrII CCTAGG 1 cut(s) 553
AxyI CCTNAGG 1 cut(s) 824
BalI TGGCCA 1 cut(s) 146
Bbv12I GWGCWC 2 cut(s) 699, 766
BbvI GCAGC 1 cut(s) 70
BccI CCATC 3 cut(s) 5, 144, 266
BcgI CGANNNNNNTGC 2 cut(s) 11, 45
BciT130I CCWGG 2 cut(s) 476, 584
BfaI CTAG 2 cut(s) 554, 852
BisI GCNGC 2 cut(s) 84, 576
BlnI CCTAGG 1 cut(s) 553
BlsI GCNGC 2 cut(s) 85, 577
Bme1390I CCNGG 2 cut(s) 476, 584
Bme18I GGWCC 1 cut(s) 788
BmgT120I GGNCC 2 cut(s) 288, 788
BmiI GGNNCC 1 cut(s) 290
BmrFI CCNGG 2 cut(s) 476, 584
BmrI ACTGGG 1 cut(s) 287
BmsI GCATC 1 cut(s) 415
BmuI ACTGGG 1 cut(s) 287
BpmI CTGGAG 1 cut(s) 750
BsaHI GRCGYC 1 cut(s) 558
BsaJI CCNNGG 5 cut(s) 321, 373, 526, 553, 812
BsaXI ACNNNNNCTCC 2 cut(s) 247, 277
Bsc4I CCNNNNNNNGG 4 cut(s) 196, 272, 296, 297
Bse1I ACTGG 5 cut(s) 10, 255, 293, 369, 841
Bse21I CCTNAGG 1 cut(s) 824
BseBI CCWGG 2 cut(s) 476, 584
BseDI CCNNGG 5 cut(s) 321, 373, 526, 553, 812
BseGI GGATG 2 cut(s) 121, 161
BseLI CCNNNNNNNGG 4 cut(s) 196, 272, 296, 297
BseMII CTCAG 2 cut(s) 423, 815
BseNI ACTGG 5 cut(s) 10, 255, 293, 369, 841
BseXI GCAGC 1 cut(s) 70
Bsh1236I CGCG 1 cut(s) 528
BshFI GGCC 2 cut(s) 146, 289
BsiHKAI GWGCWC 2 cut(s) 699, 766
BslFI GGGAC 2 cut(s) 28, 168
BslI CCNNNNNNNGG 4 cut(s) 196, 272, 296, 297
BsmFI GGGAC 2 cut(s) 28, 168
BsmI GAATGC 1 cut(s) 461
BsnI GGCC 2 cut(s) 146, 289
Bsp1286I GDGCHC 2 cut(s) 699, 766
Bsp143I GATC 2 cut(s) 179, 668
BspACI CCGC 4 cut(s) 197, 526, 528, 575
BspANI GGCC 2 cut(s) 146, 289
BspCNI CTCAG 2 cut(s) 424, 816
BspFNI CGCG 1 cut(s) 528
BspLI GGNNCC 1 cut(s) 290
BspPI GGATC 2 cut(s) 187, 676
BsrI ACTGG 5 cut(s) 10, 255, 293, 369, 841
BssECI CCNNGG 5 cut(s) 321, 373, 526, 553, 812
BssMI GATC 2 cut(s) 179, 668
BssNI GRCGYC 1 cut(s) 558
BssT1I CCWWGG 4 cut(s) 321, 373, 553, 812
Bst2UI CCWGG 2 cut(s) 476, 584
Bst4CI ACNGT 4 cut(s) 448, 497, 625, 792
BstACI GRCGYC 1 cut(s) 558
BstAPI GCANNNNNTGC 1 cut(s) 780
BstC8I GCNNGC 2 cut(s) 638, 680
BstDEI CTNAG 3 cut(s) 432, 632, 824
BstDSI CCRYGG 1 cut(s) 526
BstF5I GGATG 2 cut(s) 121, 161
BstFNI CGCG 1 cut(s) 528
BstHHI GCGC 1 cut(s) 857
BstKTI GATC 2 cut(s) 182, 671
BstMBI GATC 2 cut(s) 179, 668
BstMWI GCNNNNNNNGC 3 cut(s) 239, 646, 780
BstNI CCWGG 2 cut(s) 476, 584
BstNSI RCATGY 1 cut(s) 682
BstSCI CCNGG 2 cut(s) 474, 582
BstUI CGCG 1 cut(s) 528
BstV1I GCAGC 1 cut(s) 70
BstX2I RGATCY 1 cut(s) 179
BstXI CCANNNNNNTGG 1 cut(s) 636
BstYI RGATCY 1 cut(s) 179
Bsu36I CCTNAGG 1 cut(s) 824
BsuRI GGCC 2 cut(s) 146, 289
BtgI CCRYGG 1 cut(s) 526
BtsCI GGATG 2 cut(s) 121, 161
BtsIMutI CAGTG 2 cut(s) 248, 444
Cac8I GCNNGC 2 cut(s) 638, 680
CfoI GCGC 1 cut(s) 857
Cfr13I GGNCC 2 cut(s) 288, 788
Cfr42I CCGCGG 1 cut(s) 529
CseI GACGC 3 cut(s) 32, 239, 547
Csp6I GTAC 3 cut(s) 306, 621, 840
CviAII CATG 5 cut(s) 29, 220, 562, 679, 734
CviQI GTAC 3 cut(s) 306, 621, 840
DdeI CTNAG 3 cut(s) 432, 632, 824
DpnI GATC 2 cut(s) 181, 670
DpnII GATC 2 cut(s) 179, 668
DraI TTTAAA 1 cut(s) 127
EaeI YGGCCR 1 cut(s) 144
Eco130I CCWWGG 4 cut(s) 321, 373, 553, 812
Eco47I GGWCC 1 cut(s) 788
Eco81I CCTNAGG 1 cut(s) 824
EcoO109I RGGNCCY 1 cut(s) 288
EcoRII CCWGG 2 cut(s) 474, 582
EcoT14I CCWWGG 4 cut(s) 321, 373, 553, 812
ErhI CCWWGG 4 cut(s) 321, 373, 553, 812
FaeI CATG 5 cut(s) 32, 223, 565, 682, 737
FaqI GGGAC 2 cut(s) 28, 168
FatI CATG 5 cut(s) 28, 219, 561, 678, 733
Fnu4HI GCNGC 2 cut(s) 84, 576
FokI GGATG 2 cut(s) 128, 148
Fsp4HI GCNGC 2 cut(s) 84, 576
FspBI CTAG 2 cut(s) 554, 852
GlaI GCGC 1 cut(s) 856
GluI GCNGC 2 cut(s) 84, 576
GsuI CTGGAG 1 cut(s) 750
HaeIII GGCC 2 cut(s) 146, 289
HgaI GACGC 3 cut(s) 32, 239, 547
HhaI GCGC 1 cut(s) 857
Hin1I GRCGYC 1 cut(s) 558
Hin1II CATG 5 cut(s) 32, 223, 565, 682, 737
Hin6I GCGC 1 cut(s) 855
HinP1I GCGC 1 cut(s) 855
HindIII AAGCTT 1 cut(s) 310
HinfI GANTC 2 cut(s) 41, 848
HphI GGTGA 4 cut(s) 20, 226, 310, 646
Hpy166II GTNNAC 2 cut(s) 217, 831
Hpy188I TCNGA 4 cut(s) 46, 351, 424, 489
Hpy188III TCNNGA 5 cut(s) 177, 500, 767, 823, 864
Hpy8I GTNNAC 2 cut(s) 217, 831
Hpy99I CGWCG 1 cut(s) 26
HpyAV CCTTC 2 cut(s) 731, 765
HpyCH4III ACNGT 4 cut(s) 448, 497, 625, 792
HpyCH4IV ACGT 2 cut(s) 157, 860
HpyCH4V TGCA 5 cut(s) 202, 428, 570, 689, 783
HpyF10VI GCNNNNNNNGC 3 cut(s) 239, 646, 780
HpyF3I CTNAG 3 cut(s) 432, 632, 824
HpySE526I ACGT 2 cut(s) 157, 860
Hsp92I GRCGYC 1 cut(s) 558
Hsp92II CATG 5 cut(s) 32, 223, 565, 682, 737
HspAI GCGC 1 cut(s) 855
KspI CCGCGG 1 cut(s) 529
Kzo9I GATC 2 cut(s) 179, 668
LmnI GCTCC 2 cut(s) 608, 769
Lsp1109I GCAGC 1 cut(s) 70
LweI GCATC 1 cut(s) 415
MaeI CTAG 2 cut(s) 554, 852
MaeII ACGT 2 cut(s) 157, 860
MaeIII GTNAC 1 cut(s) 500
MalI GATC 2 cut(s) 181, 670
MboI GATC 2 cut(s) 179, 668
MboII GAAGA 3 cut(s) 174, 409, 523
MflI RGATCY 1 cut(s) 179
MhlI GDGCHC 2 cut(s) 699, 766
MlsI TGGCCA 1 cut(s) 146
MluCI AATT 4 cut(s) 121, 385, 701, 723
MluNI TGGCCA 1 cut(s) 146
MlyI GAGTC 1 cut(s) 50
MnlI CCTC 9 cut(s) 73, 175, 200, 273, 294, 327, 427, 763, 819
Mox20I TGGCCA 1 cut(s) 146
MroXI GAANNNNTTC 1 cut(s) 750
MscI TGGCCA 1 cut(s) 146
MseI TTAA 5 cut(s) 126, 441, 483, 704, 726
MslI CAYNNNNRTG 2 cut(s) 197, 224
Msp20I TGGCCA 1 cut(s) 146
MspA1I CMGCKG 4 cut(s) 197, 528, 543, 575
MspR9I CCNGG 2 cut(s) 476, 584
Mva1269I GAATGC 1 cut(s) 461
MvaI CCWGG 2 cut(s) 476, 584
MvnI CGCG 1 cut(s) 528
MwoI GCNNNNNNNGC 3 cut(s) 239, 646, 780
NdeII GATC 2 cut(s) 179, 668
NlaIII CATG 5 cut(s) 32, 223, 565, 682, 737
NlaIV GGNNCC 1 cut(s) 290
NmuCI GTSAC 1 cut(s) 500
NspI RCATGY 1 cut(s) 682
OliI CACNNNNGTG 1 cut(s) 197
PaeI GCATGC 1 cut(s) 682
PctI GAATGC 1 cut(s) 461
PdmI GAANNNNTTC 1 cut(s) 750
PfeI GAWTC 1 cut(s) 848
PflFI GACNNNGTC 1 cut(s) 792
PflMI CCANNNNNTGG 1 cut(s) 272
PkrI GCNGC 2 cut(s) 85, 577
PleI GAGTC 1 cut(s) 49
PpsI GAGTC 1 cut(s) 49
PshBI ATTAAT 1 cut(s) 704
Psp6I CCWGG 2 cut(s) 474, 582
PspGI CCWGG 2 cut(s) 474, 582
PspN4I GGNNCC 1 cut(s) 290
PspPI GGNCC 2 cut(s) 288, 788
PsuI RGATCY 1 cut(s) 179
PsyI GACNNNGTC 1 cut(s) 792
PvuII CAGCTG 1 cut(s) 543
RsaI GTAC 3 cut(s) 307, 622, 841
RsaNI GTAC 3 cut(s) 306, 621, 840
RseI CAYNNNNRTG 2 cut(s) 197, 224
SacII CCGCGG 1 cut(s) 529
SaqAI TTAA 5 cut(s) 126, 441, 483, 704, 726
SatI GCNGC 2 cut(s) 84, 576
Sau3AI GATC 2 cut(s) 179, 668
Sau96I GGNCC 2 cut(s) 288, 788
SchI GAGTC 1 cut(s) 50
ScrFI CCNGG 2 cut(s) 476, 584
SduI GDGCHC 2 cut(s) 699, 766
SfaNI GCATC 1 cut(s) 415
Sfr303I CCGCGG 1 cut(s) 529
SgrBI CCGCGG 1 cut(s) 529
SinI GGWCC 1 cut(s) 788
SmiMI CAYNNNNRTG 2 cut(s) 197, 224
SphI GCATGC 1 cut(s) 682
Sse9I AATT 4 cut(s) 121, 385, 701, 723
SsiI CCGC 4 cut(s) 197, 526, 528, 575
SspMI CTAG 2 cut(s) 554, 852
StyD4I CCNGG 2 cut(s) 474, 582
StyI CCWWGG 4 cut(s) 321, 373, 553, 812
TaaI ACNGT 4 cut(s) 448, 497, 625, 792
TaiI ACGT 2 cut(s) 160, 863
TaqI TCGA 1 cut(s) 714
TasI AATT 4 cut(s) 121, 385, 701, 723
TatI WGTACW 1 cut(s) 620
TauI GCSGC 1 cut(s) 578
TfiI GAWTC 1 cut(s) 848
Tru1I TTAA 5 cut(s) 126, 441, 483, 704, 726
Tru9I TTAA 5 cut(s) 126, 441, 483, 704, 726
TscAI CASTG 2 cut(s) 255, 451
TseFI GTSAC 1 cut(s) 500
TseI GCWGC 1 cut(s) 83
Tsp45I GTSAC 1 cut(s) 500
TspDTI ATGAA 4 cut(s) 150, 304, 750, 759
TspGWI ACGGA 1 cut(s) 92
TspRI CASTG 2 cut(s) 255, 451
Tth111I GACNNNGTC 1 cut(s) 792
Van91I CCANNNNNTGG 1 cut(s) 272
VpaK11BI GGWCC 1 cut(s) 788
VspI ATTAAT 1 cut(s) 704
XceI RCATGY 1 cut(s) 682
XmaJI CCTAGG 1 cut(s) 553
XmnI GAANNNNTTC 1 cut(s) 750
XspI CTAG 2 cut(s) 554, 852
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.