RchiOBHm_Chr3g0483401

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0005549
Physical Location & Seq
Forward (+)
29619454 .. 29624422
4969 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44815

Sequence Viewer

Length: 1491 bp
ATGGAGAACTACTACTTGAAAAAGCACTTGGAAGCTGAGCAGAAGCACCTTTCCACGGATTCTCGACGTAAATGGAGGAAAGCAGCCGCTCTTCTCCGTTCTAGAAGGTTCCGTGGTGTCGTTGATCTGGCAAACCAGGCCAGGGCTGAGATACAGGGTTCAGTTGAAGTTGCTGTTAAGGTTCAAAGAGCAGCAATACTACAGTTTGACAAAGATGAGCTTGCATCTATCAAACGTGACCATGATATTGGGTCAGGTCAAAATATGGTGGCAGACAATGAACTTGCTATGGGTATAGCAGGAACCCAGGTTGCCAGAGGAGGTTCTGATGTTATCAAATTGGATGACAATTTTAGAACTGCAGCAAGTGTGGCCAGATGGAATCGTGCATACATAAACATCCAAAATTTTATGCAGTTCCAGTTAGCAATTAACGTAGTTGCTCTGGTGATCAATGCTGTTTCTGGACGTGTTTCAGGAAACGTTCCCCTTGCTACTGTGCAATTACTTTGGGTCAACCTGAATATGTACACTGTTGGTGCATGCGCACTTATACAAATGCCCCAGTTTTTGACGGGTACAAGCTTCATCACCAGGGGCATTTGGAGGAATATCATTGGCCAGAGTATCTATCAACTGACTGTCCTTGGAGTTCTCAATTTCTATGGGACGCAGCTACTAGGACTTACTAGTCCAGATGCAATTGCTGTTCTTGACACTGTGATATTCAACTCAATAGTGTTTTGCAAGATGCTTCAGATGAATAGGCATGAAATAGAGATGATAAACACATTTGACAACTGGATATTGGTAGGAATCATGATTTGCACTTTGGCCTTTGAAGTTGTCATAGTTGAGTTCTTGGGAGTGGTTGCTAGCATTGTCCCACTATGGGGACTCACCATTTTTATTGGATCAGTTAGCATGACTGTTGCATTTGTCCCGGAGTGCATGCCTGTTGGAAACTCAGCCAAAGGGACTGGTGATGGCTTGGCCACAGAAGGACCGGAAATTCATAGATTCTCCGCTGAGCAGCTGAATGCTGTTAGGAAGTTGGTCCAGGAAAATTTCCAAGTCGCTTTTCATGGTCCAAAAACGGCACTACTGAAGATCAATTACAGTGCCATATTTAGATCTGTTGGTGAAGTTAGAATCTCAGCTCAAATGATGCAATGCACCTTTGTGGTGTTGGCAACTTTTGTGGTTATTGTAAGGGTTGGGGATGACATTGATTTTGCCTTCTCCATGCTGACATTTTTGGTGAAGCAGAATATTAATGGTATTACAACCATCGTCAAATTTTGGGTTGCTTTTGCGGTTGACTATGGTTATGCCTCCGTGGTGATAGCTTTACTGGTAATGATAAAAATAGTGGTGGAAAAAGTGCGTTGCAACGAGATCAATAATTGGTCTTCGGGAGATGCAACTAGATTTGTCGTCGATTATGCTCTTGCAATGTTAGAGTATTTACTCTTCCATAAAATAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009624 GO:0009628 GO:0009705 GO:0010035 GO:0010941 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0042742 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055081 GO:0055085 GO:0060548 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098542 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

496

Amino Acids

55.04

Weight (kDa)

8.43

Isoelectric Point (pI)

29.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_ATPase_C PF00689 162 - 312 6.6e-09 Cation transporting ATPase, C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 690
Acc16I TGCGCA 1 cut(s) 547
AccBSI CCGCTC 1 cut(s) 89
AciI CCGC 3 cut(s) 87, 1026, 1316
AclI AACGTT 1 cut(s) 483
AclWI GGATC 1 cut(s) 922
AcoI YGGCCR 3 cut(s) 372, 619, 993
AcsI RAATTY 4 cut(s) 406, 1011, 1066, 1298
AcuI CTGAAG 2 cut(s) 740, 1127
AfaI GTAC 2 cut(s) 530, 580
AfiI CCNNNNNNNGG 4 cut(s) 55, 142, 892, 893
AflIII ACRYGT 1 cut(s) 469
AgsI TTSAA 5 cut(s) 19, 167, 185, 730, 842
AhlI ACTAGT 1 cut(s) 689
AjiI CACGTC 1 cut(s) 470
AjnI CCWGG 5 cut(s) 135, 140, 306, 593, 1059
AjuI GAANNNNNNNTTGG 4 cut(s) 11, 43, 1065, 1097
AleI CACNNNNGTG 1 cut(s) 1181
AluBI AGCT 7 cut(s) 35, 220, 585, 676, 1036, 1160, 1349
AluI AGCT 7 cut(s) 35, 220, 585, 676, 1036, 1160, 1349
AlwI GGATC 1 cut(s) 922
AoxI GGCC 5 cut(s) 138, 372, 619, 834, 993
ApeKI GCWGC 5 cut(s) 83, 191, 362, 673, 1033
ApoI RAATTY 4 cut(s) 406, 1011, 1066, 1298
ArsI GACNNNNNNTTYG 2 cut(s) 1217, 1249
AseI ATTAAT 1 cut(s) 1275
AspLEI GCGC 1 cut(s) 548
AspS9I GGNCC 3 cut(s) 1004, 1057, 1088
AsuC2I CCSGG 1 cut(s) 944
AsuHPI GGTGA 7 cut(s) 460, 583, 892, 995, 1154, 1273, 1354
AsuNHI GCTAGC 1 cut(s) 875
AvaII GGWCC 3 cut(s) 1004, 1057, 1088
BalI TGGCCA 3 cut(s) 374, 621, 995
BarI GAAGNNNNNNTAC 4 cut(s) 1100, 1132, 1457, 1489
BbsI GAAGAC 1 cut(s) 1404
BbvI GCAGC 5 cut(s) 95, 203, 374, 685, 1045
BccI CCATC 3 cut(s) 372, 980, 1298
BceAI ACGGC 1 cut(s) 1113
BciT130I CCWGG 5 cut(s) 137, 142, 308, 595, 1061
BclI TGATCA 1 cut(s) 450
BcnI CCSGG 1 cut(s) 944
BcuI ACTAGT 1 cut(s) 689
BfaI CTAG 5 cut(s) 102, 680, 690, 876, 1428
BfmI CTRYAG 2 cut(s) 200, 360
BglII AGATCT 1 cut(s) 1133
BisI GCNGC 6 cut(s) 84, 87, 192, 363, 674, 1034
BlpI GCTNAGC 2 cut(s) 36, 1029
BlsI GCNGC 6 cut(s) 85, 88, 193, 364, 675, 1035
Bme1390I CCNGG 6 cut(s) 137, 142, 308, 595, 944, 1061
Bme18I GGWCC 3 cut(s) 1004, 1057, 1088
BmgBI CACGTC 1 cut(s) 470
BmgT120I GGNCC 3 cut(s) 1004, 1057, 1088
BmiI GGNNCC 2 cut(s) 110, 304
BmrFI CCNGG 6 cut(s) 137, 142, 308, 595, 944, 1061
BmrI ACTGGG 1 cut(s) 559
BmsI GCATC 5 cut(s) 233, 688, 741, 1158, 1411
BmtI GCTAGC 1 cut(s) 879
BmuI ACTGGG 1 cut(s) 559
BpiI GAAGAC 1 cut(s) 1404
Bpu1102I GCTNAGC 2 cut(s) 36, 1029
BpuMI CCSGG 1 cut(s) 944
BsaJI CCNNGG 7 cut(s) 54, 112, 141, 306, 594, 646, 1338
BsaWI WCCGGW 1 cut(s) 1006
Bsc4I CCNNNNNNNGG 4 cut(s) 55, 142, 892, 893
Bse1I ACTGG 5 cut(s) 421, 565, 806, 985, 1359
Bse3DI GCAATG 2 cut(s) 1178, 1461
BseBI CCWGG 5 cut(s) 137, 142, 308, 595, 1061
BseDI CCNNGG 7 cut(s) 54, 112, 141, 306, 594, 646, 1338
BseGI GGATG 3 cut(s) 349, 399, 1228
BseLI CCNNNNNNNGG 4 cut(s) 55, 142, 892, 893
BseMI GCAATG 2 cut(s) 1178, 1461
BseMII CTCAG 5 cut(s) 27, 138, 981, 1020, 1170
BseNI ACTGG 5 cut(s) 421, 565, 806, 985, 1359
BseRI GAGGAG 1 cut(s) 333
BseXI GCAGC 5 cut(s) 95, 203, 374, 685, 1045
BshFI GGCC 5 cut(s) 140, 374, 621, 836, 995
BsiSI CCGG 2 cut(s) 944, 1007
BslFI GGGAC 5 cut(s) 682, 869, 909, 926, 991
BslI CCNNNNNNNGG 4 cut(s) 55, 142, 892, 893
BsmFI GGGAC 5 cut(s) 682, 869, 909, 926, 991
BsmI GAATGC 1 cut(s) 1045
BsnI GGCC 5 cut(s) 140, 374, 621, 836, 995
Bsp1407I TGTACA 1 cut(s) 528
Bsp143I GATC 6 cut(s) 124, 450, 914, 1110, 1133, 1398
Bsp1720I GCTNAGC 2 cut(s) 36, 1029
BspACI CCGC 3 cut(s) 87, 1026, 1316
BspANI GGCC 5 cut(s) 140, 374, 621, 836, 995
BspCNI CTCAG 5 cut(s) 28, 139, 980, 1021, 1169
BspHI TCATGA 1 cut(s) 819
BspLI GGNNCC 2 cut(s) 110, 304
BspMAI CTGCAG 1 cut(s) 364
BspOI GCTAGC 1 cut(s) 879
BspPI GGATC 1 cut(s) 922
BspQI GCTCTTC 1 cut(s) 96
BsrBI CCGCTC 1 cut(s) 89
BsrDI GCAATG 2 cut(s) 1178, 1461
BsrGI TGTACA 1 cut(s) 528
BsrI ACTGG 5 cut(s) 421, 565, 806, 985, 1359
BssECI CCNNGG 7 cut(s) 54, 112, 141, 306, 594, 646, 1338
BssMI GATC 6 cut(s) 124, 450, 914, 1110, 1133, 1398
BssT1I CCWWGG 1 cut(s) 646
Bst2UI CCWGG 5 cut(s) 137, 142, 308, 595, 1061
Bst4CI ACNGT 7 cut(s) 204, 499, 535, 643, 721, 931, 1121
Bst6I CTCTTC 2 cut(s) 96, 1478
BstAUI TGTACA 1 cut(s) 528
BstC8I GCNNGC 4 cut(s) 222, 544, 877, 953
BstDEI CTNAG 5 cut(s) 36, 147, 967, 1029, 1156
BstDSI CCRYGG 3 cut(s) 54, 112, 1338
BstF5I GGATG 3 cut(s) 349, 399, 1228
BstHHI GCGC 1 cut(s) 548
BstKTI GATC 6 cut(s) 127, 453, 917, 1113, 1136, 1401
BstMBI GATC 6 cut(s) 124, 450, 914, 1110, 1133, 1398
BstMWI GCNNNNNNNGC 2 cut(s) 137, 371
BstNI CCWGG 5 cut(s) 137, 142, 308, 595, 1061
BstNSI RCATGY 2 cut(s) 546, 955
BstSCI CCNGG 6 cut(s) 135, 140, 306, 593, 942, 1059
BstSFI CTRYAG 2 cut(s) 200, 360
BstV1I GCAGC 5 cut(s) 95, 203, 374, 685, 1045
BstV2I GAAGAC 1 cut(s) 1404
BstX2I RGATCY 1 cut(s) 1133
BstXI CCANNNNNNTGG 1 cut(s) 248
BstYI RGATCY 1 cut(s) 1133
BsuRI GGCC 5 cut(s) 140, 374, 621, 836, 995
BtgI CCRYGG 3 cut(s) 54, 112, 1338
BtrI CACGTC 1 cut(s) 470
BtsCI GGATG 3 cut(s) 349, 399, 1228
BtsIMutI CAGTG 3 cut(s) 531, 717, 1126
Cac8I GCNNGC 4 cut(s) 222, 544, 877, 953
CciI TCATGA 1 cut(s) 819
CfoI GCGC 1 cut(s) 548
Cfr13I GGNCC 3 cut(s) 1004, 1057, 1088
CseI GACGC 1 cut(s) 679
Csp6I GTAC 2 cut(s) 529, 579
CspCI CAANNNNNGTGG 2 cut(s) 1182, 1217
CviAII CATG 8 cut(s) 242, 543, 770, 820, 925, 952, 1085, 1246
CviQI GTAC 2 cut(s) 529, 579
DdeI CTNAG 5 cut(s) 36, 147, 967, 1029, 1156
DpnI GATC 6 cut(s) 126, 452, 916, 1112, 1135, 1400
DpnII GATC 6 cut(s) 124, 450, 914, 1110, 1133, 1398
DrdI GACNNNNNNGTC 1 cut(s) 690
DseDI GACNNNNNNGTC 1 cut(s) 690
EaeI YGGCCR 3 cut(s) 372, 619, 993
Eam1104I CTCTTC 2 cut(s) 96, 1478
EarI CTCTTC 2 cut(s) 96, 1478
Eco130I CCWWGG 1 cut(s) 646
Eco47I GGWCC 3 cut(s) 1004, 1057, 1088
Eco57I CTGAAG 2 cut(s) 740, 1127
EcoRII CCWGG 5 cut(s) 135, 140, 306, 593, 1059
EcoT14I CCWWGG 1 cut(s) 646
ErhI CCWWGG 1 cut(s) 646
FaeI CATG 8 cut(s) 245, 546, 773, 823, 928, 955, 1088, 1249
FalI AAGNNNNNCTT 2 cut(s) 204, 236
FaqI GGGAC 5 cut(s) 682, 869, 909, 926, 991
FatI CATG 8 cut(s) 241, 542, 769, 819, 924, 951, 1084, 1245
FbaI TGATCA 1 cut(s) 450
Fnu4HI GCNGC 6 cut(s) 84, 87, 192, 363, 674, 1034
FokI GGATG 3 cut(s) 356, 386, 1235
Fsp4HI GCNGC 6 cut(s) 84, 87, 192, 363, 674, 1034
FspAI RTGCGCAY 1 cut(s) 547
FspBI CTAG 5 cut(s) 102, 680, 690, 876, 1428
FspI TGCGCA 1 cut(s) 547
GlaI GCGC 1 cut(s) 547
GluI GCNGC 6 cut(s) 84, 87, 192, 363, 674, 1034
HaeIII GGCC 5 cut(s) 140, 374, 621, 836, 995
HapII CCGG 2 cut(s) 944, 1007
HgaI GACGC 1 cut(s) 679
HhaI GCGC 1 cut(s) 548
Hin1II CATG 8 cut(s) 245, 546, 773, 823, 928, 955, 1088, 1249
Hin6I GCGC 1 cut(s) 546
HinP1I GCGC 1 cut(s) 546
HincII GTYRAC 2 cut(s) 517, 1321
HindII GTYRAC 2 cut(s) 517, 1321
HindIII AAGCTT 1 cut(s) 583
HinfI GANTC 6 cut(s) 59, 382, 816, 897, 1020, 1152
HpaII CCGG 2 cut(s) 944, 1007
HphI GGTGA 7 cut(s) 460, 583, 892, 995, 1154, 1273, 1354
Hpy166II GTNNAC 3 cut(s) 517, 531, 1321
Hpy188I TCNGA 2 cut(s) 328, 759
Hpy188III TCNNGA 8 cut(s) 63, 102, 465, 477, 695, 713, 820, 1416
Hpy8I GTNNAC 3 cut(s) 517, 531, 1321
Hpy99I CGWCG 2 cut(s) 69, 1442
HpyAV CCTTC 3 cut(s) 99, 995, 1249
HpyCH4III ACNGT 7 cut(s) 204, 499, 535, 643, 721, 931, 1121
HpyCH4IV ACGT 5 cut(s) 67, 235, 435, 469, 483
HpyF10VI GCNNNNNNNGC 2 cut(s) 137, 371
HpyF3I CTNAG 5 cut(s) 36, 147, 967, 1029, 1156
HpySE526I ACGT 5 cut(s) 67, 235, 435, 469, 483
Hsp92II CATG 8 cut(s) 245, 546, 773, 823, 928, 955, 1088, 1249
HspAI GCGC 1 cut(s) 546
Ksp22I TGATCA 1 cut(s) 450
Kzo9I GATC 6 cut(s) 124, 450, 914, 1110, 1133, 1398
LguI GCTCTTC 1 cut(s) 96
Lsp1109I GCAGC 5 cut(s) 95, 203, 374, 685, 1045
LweI GCATC 5 cut(s) 233, 688, 741, 1158, 1411
MaeI CTAG 5 cut(s) 102, 680, 690, 876, 1428
MaeII ACGT 5 cut(s) 67, 235, 435, 469, 483
MaeIII GTNAC 1 cut(s) 236
MalI GATC 6 cut(s) 126, 452, 916, 1112, 1135, 1400
MbiI CCGCTC 1 cut(s) 89
MboI GATC 6 cut(s) 124, 450, 914, 1110, 1133, 1398
MboII GAAGA 4 cut(s) 83, 1120, 1404, 1465
MfeI CAATTG 1 cut(s) 702
MflI RGATCY 1 cut(s) 1133
MlsI TGGCCA 3 cut(s) 374, 621, 995
MluNI TGGCCA 3 cut(s) 374, 621, 995
MlyI GAGTC 1 cut(s) 891
MmeI TCCRAC 1 cut(s) 940
MnlI CCTC 5 cut(s) 69, 311, 314, 600, 1345
Mox20I TGGCCA 3 cut(s) 374, 621, 995
MscI TGGCCA 3 cut(s) 374, 621, 995
MseI TTAA 3 cut(s) 177, 432, 1275
MslI CAYNNNNRTG 1 cut(s) 1181
Msp20I TGGCCA 3 cut(s) 374, 621, 995
MspA1I CMGCKG 2 cut(s) 1028, 1036
MspI CCGG 2 cut(s) 944, 1007
MspR9I CCNGG 6 cut(s) 137, 142, 308, 595, 944, 1061
MunI CAATTG 1 cut(s) 702
Mva1269I GAATGC 1 cut(s) 1045
MvaI CCWGG 5 cut(s) 137, 142, 308, 595, 1061
MwoI GCNNNNNNNGC 2 cut(s) 137, 371
NciI CCSGG 1 cut(s) 944
NdeII GATC 6 cut(s) 124, 450, 914, 1110, 1133, 1398
NheI GCTAGC 1 cut(s) 875
NlaIII CATG 8 cut(s) 245, 546, 773, 823, 928, 955, 1088, 1249
NlaIV GGNNCC 2 cut(s) 110, 304
NmuCI GTSAC 1 cut(s) 236
NsbI TGCGCA 1 cut(s) 547
NspI RCATGY 2 cut(s) 546, 955
OliI CACNNNNGTG 1 cut(s) 1181
PaeI GCATGC 2 cut(s) 546, 955
PagI TCATGA 1 cut(s) 819
PciSI GCTCTTC 1 cut(s) 96
PctI GAATGC 1 cut(s) 1045
PfeI GAWTC 5 cut(s) 59, 382, 816, 1020, 1152
PfoI TCCNGGA 2 cut(s) 942, 1059
PkrI GCNGC 6 cut(s) 85, 88, 193, 364, 675, 1035
PleI GAGTC 1 cut(s) 891
PpsI GAGTC 1 cut(s) 891
PshBI ATTAAT 1 cut(s) 1275
Psp1406I AACGTT 1 cut(s) 483
Psp6I CCWGG 5 cut(s) 135, 140, 306, 593, 1059
PspGI CCWGG 5 cut(s) 135, 140, 306, 593, 1059
PspN4I GGNNCC 2 cut(s) 110, 304
PspPI GGNCC 3 cut(s) 1004, 1057, 1088
PstI CTGCAG 1 cut(s) 364
PsuI RGATCY 1 cut(s) 1133
PvuII CAGCTG 1 cut(s) 1036
RsaI GTAC 2 cut(s) 530, 580
RsaNI GTAC 2 cut(s) 529, 579
RseI CAYNNNNRTG 1 cut(s) 1181
SapI GCTCTTC 1 cut(s) 96
SaqAI TTAA 3 cut(s) 177, 432, 1275
SatI GCNGC 6 cut(s) 84, 87, 192, 363, 674, 1034
Sau3AI GATC 6 cut(s) 124, 450, 914, 1110, 1133, 1398
Sau96I GGNCC 3 cut(s) 1004, 1057, 1088
SchI GAGTC 1 cut(s) 891
ScrFI CCNGG 6 cut(s) 137, 142, 308, 595, 944, 1061
SfaNI GCATC 5 cut(s) 233, 688, 741, 1158, 1411
SfcI CTRYAG 2 cut(s) 200, 360
SinI GGWCC 3 cut(s) 1004, 1057, 1088
SmiMI CAYNNNNRTG 1 cut(s) 1181
SpeI ACTAGT 1 cut(s) 689
SphI GCATGC 2 cut(s) 546, 955
SsiI CCGC 3 cut(s) 87, 1026, 1316
SspI AATATT 1 cut(s) 1273
SspMI CTAG 5 cut(s) 102, 680, 690, 876, 1428
StyD4I CCNGG 6 cut(s) 135, 140, 306, 593, 942, 1059
StyI CCWWGG 1 cut(s) 646
TaaI ACNGT 7 cut(s) 204, 499, 535, 643, 721, 931, 1121
TaiI ACGT 5 cut(s) 70, 238, 438, 472, 486
TaqI TCGA 2 cut(s) 64, 1440
TatI WGTACW 1 cut(s) 528
TauI GCSGC 1 cut(s) 89
TfiI GAWTC 5 cut(s) 59, 382, 816, 1020, 1152
Tru1I TTAA 3 cut(s) 177, 432, 1275
Tru9I TTAA 3 cut(s) 177, 432, 1275
TscAI CASTG 3 cut(s) 538, 724, 1126
TseFI GTSAC 1 cut(s) 236
TseI GCWGC 5 cut(s) 83, 191, 362, 673, 1033
Tsp45I GTSAC 1 cut(s) 236
TspDTI ATGAA 6 cut(s) 294, 577, 776, 786, 1004, 1073
TspGWI ACGGA 4 cut(s) 71, 86, 101, 1327
TspRI CASTG 3 cut(s) 538, 724, 1126
VpaK11BI GGWCC 3 cut(s) 1004, 1057, 1088
VspI ATTAAT 1 cut(s) 1275
XapI RAATTY 4 cut(s) 406, 1011, 1066, 1298
XbaI TCTAGA 1 cut(s) 101
XceI RCATGY 2 cut(s) 546, 955
XspI CTAG 5 cut(s) 102, 680, 690, 876, 1428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.