Rw3G022950

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0005549
Physical Location & Seq
Forward (+)
28513607 .. 28515930
2324 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G022950.1

Sequence Viewer

Length: 981 bp
ATGTATGGTTCAGTTGATGTTGCTTTTAAGGTTCAACAAGCAGCATTACTACAGTTTGACGAAGGTGGTATTATCCTTAAATATATAGGTGGTGATTATAATCCAGATAAAGAGAAACCAGATGAGCTTGCATCTATCAAACGTGACCATGATATTGGGTCAGGTCAAAATTTGGTGGCAAACAATGGACTTGCTATGGGCATAGCAGGAACCCAGGTTGCCAAAGAAGGTTCTGATGTTATCATATGGGATGACAATTTTAGAACTGCAGTAAGTGTGCCCAGATGGAGCCGTGCATACATAAACATCCAAAATTTTATGCAGTTCCAGTTAGCAATTAACGTTGTTGCTCTGGTGCTCAATGCTGTTTCTGGACGTGTTTCAGACAGTGTTAAATTACACGCCACTTTCAGGAAACGTCCCCCTTACTGCTGTGCAATTGCTTTGGGTCAACCTGAATATTTTTTGAAGGGTACAAGGTTCATCACCTGGGGCATTTGGAGGAATATCATTGGTCAGAGTATCTATCAACTGACTGTCCTTGGAGTTCTCAATTTCTATGGGACGCAGCTACTAGGACTTACTAGTCCAGATGCAATTGCTGTTCTTGACACTGTGATATTCAACTCAATAGCGTTTTGCAAGGTGATTAATGAGATGATTCAGATGAATAGGCATGAAATAGAGATGATAAACACATTTGACAACTGGATATTGGTAGGAATCATAATTTGCACTTTGGCCTTCGAAGTTGTCATAGTTGAGTTCTTGGGAGTGGTTGCTAGCATTGTGCCTCTATGGGTACTCACCATTTATATTGGATCAGTTAGCATGACTGTTGCAGTTGTCCTGGAGTGCATGCCTGTTGGAAACTCAGCCAAAGGGACTGGTGATGGCTTGGCCACAGAAGGACCGGAAATTCATAGATTCTCCGCTGAGCAGCTGAATGCTGTTATGAAGTTGGTCCAGGTTAGTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009624 GO:0009628 GO:0009705 GO:0010035 GO:0010941 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0042742 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055081 GO:0055085 GO:0060548 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098542 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

326

Amino Acids

35.77

Weight (kDa)

5.44

Isoelectric Point (pI)

27.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_ATPase_C PF00689 157 - 281 1.6e-08 Cation transporting ATPase, C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 99
AasI GACNNNNNNGTC 1 cut(s) 585
AciI CCGC 1 cut(s) 933
AclI AACGTT 1 cut(s) 342
AclWI GGATC 1 cut(s) 829
AcoI YGGCCR 1 cut(s) 900
AcsI RAATTY 3 cut(s) 169, 313, 918
AfaI GTAC 2 cut(s) 475, 804
AfiI CCNNNNNNNGG 1 cut(s) 411
AflIII ACRYGT 1 cut(s) 376
AgsI TTSAA 3 cut(s) 35, 469, 625
AhlI ACTAGT 1 cut(s) 584
AjiI CACGTC 1 cut(s) 377
AjnI CCWGG 4 cut(s) 213, 488, 849, 966
AluBI AGCT 3 cut(s) 127, 571, 943
AluI AGCT 3 cut(s) 127, 571, 943
Alw21I GWGCWC 1 cut(s) 360
AlwI GGATC 1 cut(s) 829
AoxI GGCC 2 cut(s) 741, 900
ApeKI GCWGC 3 cut(s) 41, 568, 940
ApoI RAATTY 3 cut(s) 169, 313, 918
AseI ATTAAT 1 cut(s) 651
AspS9I GGNCC 2 cut(s) 911, 964
AsuHPI GGTGA 5 cut(s) 104, 478, 658, 799, 902
AsuII TTCGAA 1 cut(s) 747
AsuNHI GCTAGC 1 cut(s) 782
AvaII GGWCC 2 cut(s) 911, 964
BaeGI GKGCMC 1 cut(s) 282
BalI TGGCCA 1 cut(s) 902
Bbv12I GWGCWC 1 cut(s) 360
BbvI GCAGC 3 cut(s) 53, 580, 952
BccI CCATC 2 cut(s) 279, 887
BceAI ACGGC 1 cut(s) 276
BciT130I CCWGG 4 cut(s) 215, 490, 851, 968
BcuI ACTAGT 1 cut(s) 584
BfaI CTAG 3 cut(s) 575, 585, 783
BfmI CTRYAG 2 cut(s) 50, 267
BisI GCNGC 3 cut(s) 42, 569, 941
BlpI GCTNAGC 1 cut(s) 936
BlsI GCNGC 3 cut(s) 43, 570, 942
Bme1390I CCNGG 4 cut(s) 215, 490, 851, 968
Bme18I GGWCC 2 cut(s) 911, 964
BmgBI CACGTC 1 cut(s) 377
BmgT120I GGNCC 2 cut(s) 911, 964
BmiI GGNNCC 2 cut(s) 211, 290
BmrFI CCNGG 4 cut(s) 215, 490, 851, 968
BmsI GCATC 2 cut(s) 140, 583
BmtI GCTAGC 1 cut(s) 786
BpmI CTGGAG 1 cut(s) 872
Bpu1102I GCTNAGC 1 cut(s) 936
Bpu14I TTCGAA 1 cut(s) 747
BsaBI GATNNNNATC 1 cut(s) 99
BsaJI CCNNGG 3 cut(s) 213, 489, 541
BsaWI WCCGGW 1 cut(s) 913
Bsc4I CCNNNNNNNGG 1 cut(s) 411
Bse1I ACTGG 3 cut(s) 328, 713, 892
Bse8I GATNNNNATC 1 cut(s) 99
BseBI CCWGG 4 cut(s) 215, 490, 851, 968
BseDI CCNNGG 3 cut(s) 213, 489, 541
BseGI GGATG 2 cut(s) 256, 306
BseJI GATNNNNATC 1 cut(s) 99
BseLI CCNNNNNNNGG 1 cut(s) 411
BseMII CTCAG 2 cut(s) 888, 927
BseNI ACTGG 3 cut(s) 328, 713, 892
BseSI GKGCMC 1 cut(s) 282
BseXI GCAGC 3 cut(s) 53, 580, 952
BshFI GGCC 2 cut(s) 743, 902
BsiHKAI GWGCWC 1 cut(s) 360
BsiSI CCGG 1 cut(s) 914
BslFI GGGAC 3 cut(s) 405, 577, 898
BslI CCNNNNNNNGG 1 cut(s) 411
BsmFI GGGAC 3 cut(s) 405, 577, 898
BsmI GAATGC 1 cut(s) 952
BsnI GGCC 2 cut(s) 743, 902
Bsp119I TTCGAA 1 cut(s) 747
Bsp1286I GDGCHC 2 cut(s) 282, 360
Bsp143I GATC 1 cut(s) 821
Bsp1720I GCTNAGC 1 cut(s) 936
BspACI CCGC 1 cut(s) 933
BspANI GGCC 2 cut(s) 743, 902
BspCNI CTCAG 2 cut(s) 887, 928
BspLI GGNNCC 2 cut(s) 211, 290
BspMAI CTGCAG 1 cut(s) 271
BspOI GCTAGC 1 cut(s) 786
BspPI GGATC 1 cut(s) 829
BspT104I TTCGAA 1 cut(s) 747
BsrI ACTGG 3 cut(s) 328, 713, 892
BssECI CCNNGG 3 cut(s) 213, 489, 541
BssMI GATC 1 cut(s) 821
BssT1I CCWWGG 1 cut(s) 541
Bst2UI CCWGG 4 cut(s) 215, 490, 851, 968
Bst4CI ACNGT 5 cut(s) 54, 389, 538, 616, 838
BstBI TTCGAA 1 cut(s) 747
BstC8I GCNNGC 3 cut(s) 129, 784, 860
BstDEI CTNAG 2 cut(s) 874, 936
BstF5I GGATG 2 cut(s) 256, 306
BstKTI GATC 1 cut(s) 824
BstMBI GATC 1 cut(s) 821
BstNI CCWGG 4 cut(s) 215, 490, 851, 968
BstNSI RCATGY 1 cut(s) 862
BstSCI CCNGG 4 cut(s) 213, 488, 849, 966
BstSFI CTRYAG 2 cut(s) 50, 267
BstSLI GKGCMC 1 cut(s) 282
BstV1I GCAGC 3 cut(s) 53, 580, 952
BstXI CCANNNNNNTGG 1 cut(s) 155
BsuRI GGCC 2 cut(s) 743, 902
BtrI CACGTC 1 cut(s) 377
BtsCI GGATG 2 cut(s) 256, 306
BtsIMutI CAGTG 2 cut(s) 394, 612
Cac8I GCNNGC 3 cut(s) 129, 784, 860
Cfr13I GGNCC 2 cut(s) 911, 964
CseI GACGC 1 cut(s) 574
Csp6I GTAC 2 cut(s) 474, 803
CviAII CATG 4 cut(s) 149, 677, 832, 859
CviJI RGCY 8 cut(s) 127, 291, 571, 743, 878, 897, 902, 943
CviKI_1 RGCY 8 cut(s) 127, 291, 571, 743, 878, 897, 902, 943
CviQI GTAC 2 cut(s) 474, 803
DdeI CTNAG 2 cut(s) 874, 936
DpnI GATC 1 cut(s) 823
DpnII GATC 1 cut(s) 821
DrdI GACNNNNNNGTC 1 cut(s) 585
DseDI GACNNNNNNGTC 1 cut(s) 585
EaeI YGGCCR 1 cut(s) 900
Eco130I CCWWGG 1 cut(s) 541
Eco47I GGWCC 2 cut(s) 911, 964
EcoRII CCWGG 4 cut(s) 213, 488, 849, 966
EcoT14I CCWWGG 1 cut(s) 541
ErhI CCWWGG 1 cut(s) 541
FaeI CATG 4 cut(s) 152, 680, 835, 862
FaqI GGGAC 3 cut(s) 405, 577, 898
FatI CATG 4 cut(s) 148, 676, 831, 858
FauNDI CATATG 1 cut(s) 245
Fnu4HI GCNGC 3 cut(s) 42, 569, 941
FokI GGATG 2 cut(s) 263, 293
Fsp4HI GCNGC 3 cut(s) 42, 569, 941
FspBI CTAG 3 cut(s) 575, 585, 783
GluI GCNGC 3 cut(s) 42, 569, 941
GsuI CTGGAG 1 cut(s) 872
HaeIII GGCC 2 cut(s) 743, 902
HapII CCGG 1 cut(s) 914
HgaI GACGC 1 cut(s) 574
Hin1II CATG 4 cut(s) 152, 680, 835, 862
HincII GTYRAC 1 cut(s) 452
HindII GTYRAC 1 cut(s) 452
HinfI GANTC 3 cut(s) 661, 723, 927
HpaII CCGG 1 cut(s) 914
HphI GGTGA 5 cut(s) 104, 478, 658, 799, 902
Hpy166II GTNNAC 1 cut(s) 452
Hpy188I TCNGA 4 cut(s) 235, 385, 519, 666
Hpy188III TCNNGA 5 cut(s) 104, 372, 412, 590, 608
Hpy8I GTNNAC 1 cut(s) 452
HpyAV CCTTC 5 cut(s) 56, 221, 463, 754, 902
HpyCH4III ACNGT 5 cut(s) 54, 389, 538, 616, 838
HpyCH4IV ACGT 4 cut(s) 142, 342, 376, 418
HpyF3I CTNAG 2 cut(s) 874, 936
HpySE526I ACGT 4 cut(s) 142, 342, 376, 418
Hsp92II CATG 4 cut(s) 152, 680, 835, 862
Kzo9I GATC 1 cut(s) 821
LmnI GCTCC 1 cut(s) 288
Lsp1109I GCAGC 3 cut(s) 53, 580, 952
LweI GCATC 2 cut(s) 140, 583
MaeI CTAG 3 cut(s) 575, 585, 783
MaeII ACGT 4 cut(s) 142, 342, 376, 418
MaeIII GTNAC 1 cut(s) 143
MalI GATC 1 cut(s) 823
MboI GATC 1 cut(s) 821
MfeI CAATTG 2 cut(s) 438, 597
MhlI GDGCHC 2 cut(s) 282, 360
MlsI TGGCCA 1 cut(s) 902
MluNI TGGCCA 1 cut(s) 902
MmeI TCCRAC 1 cut(s) 847
MnlI CCTC 2 cut(s) 495, 804
Mox20I TGGCCA 1 cut(s) 902
MscI TGGCCA 1 cut(s) 902
MseI TTAA 5 cut(s) 27, 78, 339, 393, 651
Msp20I TGGCCA 1 cut(s) 902
MspA1I CMGCKG 2 cut(s) 935, 943
MspI CCGG 1 cut(s) 914
MspR9I CCNGG 4 cut(s) 215, 490, 851, 968
MunI CAATTG 2 cut(s) 438, 597
Mva1269I GAATGC 1 cut(s) 952
MvaI CCWGG 4 cut(s) 215, 490, 851, 968
NdeI CATATG 1 cut(s) 245
NdeII GATC 1 cut(s) 821
NheI GCTAGC 1 cut(s) 782
NlaIII CATG 4 cut(s) 152, 680, 835, 862
NlaIV GGNNCC 2 cut(s) 211, 290
NmuCI GTSAC 1 cut(s) 143
NspI RCATGY 1 cut(s) 862
NspV TTCGAA 1 cut(s) 747
PaeI GCATGC 1 cut(s) 862
PctI GAATGC 1 cut(s) 952
PfeI GAWTC 3 cut(s) 661, 723, 927
PfoI TCCNGGA 1 cut(s) 849
PkrI GCNGC 3 cut(s) 43, 570, 942
PshBI ATTAAT 1 cut(s) 651
PsiI TTATAA 1 cut(s) 99
Psp1406I AACGTT 1 cut(s) 342
Psp6I CCWGG 4 cut(s) 213, 488, 849, 966
PspGI CCWGG 4 cut(s) 213, 488, 849, 966
PspN4I GGNNCC 2 cut(s) 211, 290
PspPI GGNCC 2 cut(s) 911, 964
PstI CTGCAG 1 cut(s) 271
PvuII CAGCTG 1 cut(s) 943
RsaI GTAC 2 cut(s) 475, 804
RsaNI GTAC 2 cut(s) 474, 803
SaqAI TTAA 5 cut(s) 27, 78, 339, 393, 651
SatI GCNGC 3 cut(s) 42, 569, 941
Sau3AI GATC 1 cut(s) 821
Sau96I GGNCC 2 cut(s) 911, 964
ScrFI CCNGG 4 cut(s) 215, 490, 851, 968
SduI GDGCHC 2 cut(s) 282, 360
SfaNI GCATC 2 cut(s) 140, 583
SfcI CTRYAG 2 cut(s) 50, 267
SfuI TTCGAA 1 cut(s) 747
SinI GGWCC 2 cut(s) 911, 964
SpeI ACTAGT 1 cut(s) 584
SphI GCATGC 1 cut(s) 862
SsiI CCGC 1 cut(s) 933
SspI AATATT 1 cut(s) 461
SspMI CTAG 3 cut(s) 575, 585, 783
StyD4I CCNGG 4 cut(s) 213, 488, 849, 966
StyI CCWWGG 1 cut(s) 541
TaaI ACNGT 5 cut(s) 54, 389, 538, 616, 838
TaiI ACGT 4 cut(s) 145, 345, 379, 421
TaqI TCGA 1 cut(s) 747
TfiI GAWTC 3 cut(s) 661, 723, 927
Tru1I TTAA 5 cut(s) 27, 78, 339, 393, 651
Tru9I TTAA 5 cut(s) 27, 78, 339, 393, 651
TscAI CASTG 2 cut(s) 394, 619
TseFI GTSAC 1 cut(s) 143
TseI GCWGC 3 cut(s) 41, 568, 940
Tsp45I GTSAC 1 cut(s) 143
TspDTI ATGAA 5 cut(s) 472, 683, 693, 911, 971
TspRI CASTG 2 cut(s) 394, 619
VpaK11BI GGWCC 2 cut(s) 911, 964
VspI ATTAAT 1 cut(s) 651
XapI RAATTY 3 cut(s) 169, 313, 918
XceI RCATGY 1 cut(s) 862
XspI CTAG 3 cut(s) 575, 585, 783
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.