Rroxscaffold_4G00318650

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0005549
Physical Location & Seq
Forward (+)
44949648 .. 44952014
2367 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00318650.1

Sequence Viewer

Length: 375 bp
ATGGAAATTTCGACGGAAATATCGAGGATATATCAATTTCGGATTTGGAAGTCGGGAGGTACGGAGTTGAGCTCAATTTACTATAACTGCTGGTATGGTTTGATTTTTGGTGAAGTTATTGTTGTGTTTTCGAGCTTTGAATCTCAAGGTTCGATCATGATGATCCATATACCCAAATTGCTTCTTCGGCTACTTCAACCAAGTAGATTGGAACAAGAAGAAGAAGTTTCATCAAGAATGGATTACAAGCACCTTTCCGCGGATTCTCGACTTAAATGGAGTAAAGCGGTCGCTCTTCTCGTCAAGAACCGTTCGGAAGGTTCCGTGGAGTCGTTGATCCGGCAAACCAGGCGGCCGAGATACGAGTTTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

124

Amino Acids

14.54

Weight (kDa)

9.13

Isoelectric Point (pI)

62.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 260
AciI CCGC 4 cut(s) 258, 260, 287, 352
AclWI GGATC 2 cut(s) 157, 331
AcoI YGGCCR 1 cut(s) 353
AcsI RAATTY 1 cut(s) 6
AfaI GTAC 1 cut(s) 61
AfiI CCNNNNNNNGG 1 cut(s) 259
AgsI TTSAA 2 cut(s) 140, 197
AjnI CCWGG 1 cut(s) 347
AluBI AGCT 2 cut(s) 72, 135
AluI AGCT 2 cut(s) 72, 135
Alw21I GWGCWC 1 cut(s) 74
AlwI GGATC 2 cut(s) 157, 331
AoxI GGCC 1 cut(s) 353
ApoI RAATTY 1 cut(s) 6
AsuHPI GGTGA 1 cut(s) 122
BanII GRGCYC 1 cut(s) 74
Bbv12I GWGCWC 1 cut(s) 74
BciT130I CCWGG 1 cut(s) 349
BisI GCNGC 1 cut(s) 353
BlsI GCNGC 1 cut(s) 354
Bme1390I CCNGG 1 cut(s) 349
BmiI GGNNCC 1 cut(s) 322
BmrFI CCNGG 1 cut(s) 349
BplI GAGNNNNNCTC 2 cut(s) 56, 88
BpuEI CTTGAG 1 cut(s) 129
BsaJI CCNNGG 2 cut(s) 258, 324
Bsc4I CCNNNNNNNGG 1 cut(s) 259
BseBI CCWGG 1 cut(s) 349
BseDI CCNNGG 2 cut(s) 258, 324
BseLI CCNNNNNNNGG 1 cut(s) 259
BseX3I CGGCCG 1 cut(s) 353
Bsh1236I CGCG 1 cut(s) 260
Bsh1285I CGRYCG 2 cut(s) 291, 356
BshFI GGCC 1 cut(s) 355
BsiEI CGRYCG 2 cut(s) 291, 356
BsiHKAI GWGCWC 1 cut(s) 74
BsiSI CCGG 1 cut(s) 340
BslI CCNNNNNNNGG 1 cut(s) 259
BsnI GGCC 1 cut(s) 355
Bsp1286I GDGCHC 1 cut(s) 74
Bsp143I GATC 3 cut(s) 153, 162, 336
BspACI CCGC 4 cut(s) 258, 260, 287, 352
BspANI GGCC 1 cut(s) 355
BspFNI CGCG 1 cut(s) 260
BspHI TCATGA 1 cut(s) 156
BspLI GGNNCC 1 cut(s) 322
BspPI GGATC 2 cut(s) 157, 331
BspQI GCTCTTC 1 cut(s) 300
BssECI CCNNGG 2 cut(s) 258, 324
BssMI GATC 3 cut(s) 153, 162, 336
Bst2UI CCWGG 1 cut(s) 349
Bst4CI ACNGT 1 cut(s) 311
Bst6I CTCTTC 1 cut(s) 300
BstDSI CCRYGG 2 cut(s) 258, 324
BstFNI CGCG 1 cut(s) 260
BstKTI GATC 3 cut(s) 156, 165, 339
BstMBI GATC 3 cut(s) 153, 162, 336
BstMCI CGRYCG 2 cut(s) 291, 356
BstMWI GCNNNNNNNGC 2 cut(s) 187, 349
BstNI CCWGG 1 cut(s) 349
BstSCI CCNGG 1 cut(s) 347
BstUI CGCG 1 cut(s) 260
BstZI CGGCCG 1 cut(s) 353
BsuRI GGCC 1 cut(s) 355
BtgI CCRYGG 2 cut(s) 258, 324
CciI TCATGA 1 cut(s) 156
Cfr42I CCGCGG 1 cut(s) 261
Csp6I GTAC 1 cut(s) 60
CviAII CATG 1 cut(s) 157
CviJI RGCY 4 cut(s) 72, 135, 190, 355
CviKI_1 RGCY 4 cut(s) 72, 135, 190, 355
CviQI GTAC 1 cut(s) 60
DpnI GATC 3 cut(s) 155, 164, 338
DpnII GATC 3 cut(s) 153, 162, 336
EaeI YGGCCR 1 cut(s) 353
EagI CGGCCG 1 cut(s) 353
Eam1104I CTCTTC 1 cut(s) 300
EarI CTCTTC 1 cut(s) 300
Ecl136II GAGCTC 1 cut(s) 72
EclXI CGGCCG 1 cut(s) 353
Eco24I GRGCYC 1 cut(s) 74
Eco52I CGGCCG 1 cut(s) 353
Eco53kI GAGCTC 1 cut(s) 72
EcoICRI GAGCTC 1 cut(s) 72
EcoRII CCWGG 1 cut(s) 347
EcoT38I GRGCYC 1 cut(s) 74
FaeI CATG 1 cut(s) 160
FaiI YATR 6 cut(s) 31, 84, 96, 158, 168, 170
FatI CATG 1 cut(s) 156
Fnu4HI GCNGC 1 cut(s) 353
FriOI GRGCYC 1 cut(s) 74
Fsp4HI GCNGC 1 cut(s) 353
GluI GCNGC 1 cut(s) 353
HaeIII GGCC 1 cut(s) 355
HapII CCGG 1 cut(s) 340
Hin1II CATG 1 cut(s) 160
HinfI GANTC 3 cut(s) 140, 263, 329
HpaII CCGG 1 cut(s) 340
HphI GGTGA 1 cut(s) 122
Hpy188I TCNGA 2 cut(s) 42, 316
Hpy188III TCNNGA 5 cut(s) 54, 157, 234, 267, 304
Hpy99I CGWCG 1 cut(s) 16
HpyAV CCTTC 1 cut(s) 311
HpyCH4III ACNGT 1 cut(s) 311
HpyF10VI GCNNNNNNNGC 2 cut(s) 187, 349
Hsp92II CATG 1 cut(s) 160
KspI CCGCGG 1 cut(s) 261
Kzo9I GATC 3 cut(s) 153, 162, 336
LguI GCTCTTC 1 cut(s) 300
LpnPI CCDG 4 cut(s) 76, 334, 353, 361
MalI GATC 3 cut(s) 155, 164, 338
MboI GATC 3 cut(s) 153, 162, 336
MboII GAAGA 4 cut(s) 176, 230, 233, 287
MhlI GDGCHC 1 cut(s) 74
MluCI AATT 4 cut(s) 6, 35, 75, 176
MlyI GAGTC 1 cut(s) 338
MnlI CCTC 2 cut(s) 18, 50
MseI TTAA 1 cut(s) 273
MspA1I CMGCKG 1 cut(s) 260
MspI CCGG 1 cut(s) 340
MspR9I CCNGG 1 cut(s) 349
MvaI CCWGG 1 cut(s) 349
MvnI CGCG 1 cut(s) 260
MwoI GCNNNNNNNGC 2 cut(s) 187, 349
NdeII GATC 3 cut(s) 153, 162, 336
NlaIII CATG 1 cut(s) 160
NlaIV GGNNCC 1 cut(s) 322
PagI TCATGA 1 cut(s) 156
PciSI GCTCTTC 1 cut(s) 300
PcsI WCGNNNNNNNCGW 2 cut(s) 20, 297
PfeI GAWTC 2 cut(s) 140, 263
PkrI GCNGC 1 cut(s) 354
PleI GAGTC 1 cut(s) 337
PpsI GAGTC 1 cut(s) 337
Psp124BI GAGCTC 1 cut(s) 74
Psp6I CCWGG 1 cut(s) 347
PspGI CCWGG 1 cut(s) 347
PspN4I GGNNCC 1 cut(s) 322
RsaI GTAC 1 cut(s) 61
RsaNI GTAC 1 cut(s) 60
SacI GAGCTC 1 cut(s) 74
SacII CCGCGG 1 cut(s) 261
SapI GCTCTTC 1 cut(s) 300
SaqAI TTAA 1 cut(s) 273
SatI GCNGC 1 cut(s) 353
Sau3AI GATC 3 cut(s) 153, 162, 336
SchI GAGTC 1 cut(s) 338
ScrFI CCNGG 1 cut(s) 349
SduI GDGCHC 1 cut(s) 74
SetI ASST 6 cut(s) 61, 74, 137, 151, 255, 322
Sfr303I CCGCGG 1 cut(s) 261
SgrBI CCGCGG 1 cut(s) 261
SmlI CTYRAG 1 cut(s) 144
SmoI CTYRAG 1 cut(s) 144
Sse9I AATT 4 cut(s) 6, 35, 75, 176
SsiI CCGC 4 cut(s) 258, 260, 287, 352
SstI GAGCTC 1 cut(s) 74
StyD4I CCNGG 1 cut(s) 347
TaaI ACNGT 1 cut(s) 311
TaqI TCGA 5 cut(s) 11, 23, 131, 152, 268
TasI AATT 4 cut(s) 6, 35, 75, 176
TauI GCSGC 1 cut(s) 355
TfiI GAWTC 2 cut(s) 140, 263
Tru1I TTAA 1 cut(s) 273
Tru9I TTAA 1 cut(s) 273
TspDTI ATGAA 1 cut(s) 219
TspGWI ACGGA 3 cut(s) 29, 77, 313
XapI RAATTY 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.