Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0005919

Physical Location & Seq

Genomic Coordinates Reverse (-)

19138011 .. 19140483

Gene Structure 2473 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1119500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1137 bp

ATGAAGTTCCCACTTCCTTGCACAGATTGCTTCTCCTCCTCACCTAAAAAGGCCATTGAAAGAAGTATGCAAGATGGTCAAAATCCAGAAAACTTCAGAATTTTCTCTTGCAAGGAGTTGAAGTCTGCCACCAATGGCTTCCATTCCTCTAGCAAGCTTGGAGAAGGCGGATTTGGCTCTGTTTATAAGGGTCAGCTTAGAAATGGGAGCCTTGTGGCTGTGAAAGTTCTTTCCGTTGAACTCGAATCGATGAGAGGAGAGAGAGAGTTCATAGCAGAATTGGCTGCTCTGTCTGTAATCAGACATGAGAATCTTGTTAGGCTTCAAGGGTGTTGTGTTGATGGAGCTGCAAGATATCTGGTCTACGATTACATGGAAAACAATAGCCTCACACACACTTTACTTGGTGGAGAGAAAAACAGGATGAGGTTTAGTTGGGAGGCAAGACGGGGTATTTCGATTGGAGTAGCTCGAGGACTCGCCTATCTACACGAGGAAGTTGATCCTCACATTCTGCATAGAGACATTAAATCCAGCAACATTCTTCTTGATAAGAATTTCATACCGAAAGTAGGCGATTTTGGGTTATCGAAGTTGTTAAGGGACAACCATTCTCACGTTAGCACTCGTGTAGCCGGGACAATAGGTTATCTTGCTCCAGAATATGCAATTAGCGGGCGTTTGACTCGAAAATCAGATGTCTATAGCTTCGGAGTGCTGTTGTTGGAAATTGTCAGCGGCCAAGCAGTTGTGGATTTTGACATGCAACTTGGGGAGCAGTACCTGGTTCAGAAGGTATGGGAAGCACACATGGCCGGAACCCATGTACAACTAGTTGATCCTACGCTTCAGATGAACTTTCCTTCGGACGAAGCCATCAGATTCTTGAAGGTAGGCCTACTTTGTGTGCAAGAAAGGGCCAACCACCGGCCGCGAATGTCAAGGGTGGTTGAGATGTTGTCCGACAAGGAAGACATCAAGGACTCTCATATTTCGCAACCCGGACTTGTTTCGGATTTCATGGATATCAAAACGAGGCAAGAGAACTCATATGATCATAGCACTTTCTCCAAGGCCTCTACATCTAGCAGCACACAAAATCAGTATAAATCTGAGGATTATGTGTTGTCCTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

379

Amino Acids

42.39

Weight (kDa)

6.9

Isoelectric Point (pI)

31.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	47 - 316	2.1e-46	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	49 - 320	6.3e-47	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005919)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g15760 FvH4_7g19720 FvH4_7g19720
malus_domestica	MD01G1109700.v1.1 MD04G1098900.v1.1 MD12G1119500.v1.1
prunus_persica	Prupe.2G214600_v2.0.a1 Prupe.6G237400_v2.0.a1 Prupe.6G237400_v2.0.a1
pyrus_communis	pycom01g13680 pycom04g09390 pycom12g11850
rosa_chinensis	RchiOBHm_Chr1g0363561
rosa_laevigata	RLG00000027557
rosa_multiflora	Rmu_sc0000548.1_g000001 Rmu_sc0004871.1_g000014 Rmu_ssc0000433.1_g000008
rosa_roxburghii	Rroxscaffold_4G00293080 Rroxscaffold_6G00411830
rosa_rugosa	Rorug01G0308100 Rorug03G0102000 Rorug03G0102100
rosa_samantha	Rh1BG280300 Rh1CG296400 Rh1DG311500 Rh3DG200700
rosa_wichuraiana	Rw1G028100 Rw3G014260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	186
AccI	GTMKAC	1 cut(s)	363
AccII	CGCG	1 cut(s)	934
AciI	CCGC	4 cut(s)	168, 675, 738, 932
AclWI	GGATC	2 cut(s)	497, 833
AcoI	YGGCCR	3 cut(s)	739, 813, 929
AcsI	RAATTY	2 cut(s)	99, 556
AcuI	CTGAAG	2 cut(s)	79, 833
AfaI	GTAC	2 cut(s)	782, 828
AfiI	CCNNNNNNNGG	2 cut(s)	572, 927
AgsI	TTSAA	5 cut(s)	59, 121, 239, 326, 889
AhlI	ACTAGT	1 cut(s)	832
AjnI	CCWGG	1 cut(s)	783
AjuI	GAANNNNNNNTTGG	2 cut(s)	156, 188
AluBI	AGCT	5 cut(s)	157, 196, 347, 470, 708
AluI	AGCT	5 cut(s)	157, 196, 347, 470, 708
Alw26I	GTCTC	1 cut(s)	516
AlwI	GGATC	2 cut(s)	497, 833
AlwNI	CAGNNNCTG	1 cut(s)	784
Ama87I	CYCGRG	1 cut(s)	471
AoxI	GGCC	7 cut(s)	51, 739, 813, 895, 918, 929, 1074
ApeKI	GCWGC	3 cut(s)	284, 347, 1089
ApoI	RAATTY	2 cut(s)	99, 556
AspS9I	GGNCC	1 cut(s)	918
AsuC2I	CCSGG	2 cut(s)	637, 1002
AsuHPI	GGTGA	1 cut(s)	33
AvaI	CYCGRG	1 cut(s)	471
BauI	CACGAG	2 cut(s)	491, 627
BbsI	GAAGAC	1 cut(s)	978
BbvI	GCAGC	3 cut(s)	271, 334, 1101
BccI	CCATC	3 cut(s)	68, 335, 884
BciT130I	CCWGG	1 cut(s)	785
BclI	TGATCA	1 cut(s)	1054
BcnI	CCSGG	2 cut(s)	637, 1002
BcoDI	GTCTC	1 cut(s)	516
BcuI	ACTAGT	1 cut(s)	832
BfaI	CTAG	3 cut(s)	150, 833, 1086
BfmI	CTRYAG	1 cut(s)	703
BisI	GCNGC	5 cut(s)	285, 348, 739, 932, 1090
BlsI	GCNGC	5 cut(s)	286, 349, 740, 933, 1091
Bme1390I	CCNGG	3 cut(s)	637, 785, 1002
BmeT110I	CYCGRG	1 cut(s)	471
BmgT120I	GGNCC	1 cut(s)	918
BmiI	GGNNCC	2 cut(s)	209, 820
BmrFI	CCNGG	3 cut(s)	637, 785, 1002
BpiI	GAAGAC	1 cut(s)	978
BpmI	CTGGAG	1 cut(s)	642
BpuMI	CCSGG	2 cut(s)	637, 1002
Bsa29I	ATCGAT	1 cut(s)	248
BsaJI	CCNNGG	1 cut(s)	1071
BsaXI	ACNNNNNCTCC	2 cut(s)	107, 137
Bsc4I	CCNNNNNNNGG	2 cut(s)	572, 927
Bse118I	RCCGGY	1 cut(s)	927
BseBI	CCWGG	1 cut(s)	785
BseCI	ATCGAT	1 cut(s)	248
BseDI	CCNNGG	1 cut(s)	1071
BseGI	GGATG	1 cut(s)	429
BseLI	CCNNNNNNNGG	2 cut(s)	572, 927
BseMII	CTCAG	1 cut(s)	1104
BseRI	GAGGAG	3 cut(s)	25, 28, 270
BseX3I	CGGCCG	1 cut(s)	929
BseXI	GCAGC	3 cut(s)	271, 334, 1101
Bsh1236I	CGCG	1 cut(s)	934
Bsh1285I	CGRYCG	1 cut(s)	932
BshFI	GGCC	7 cut(s)	53, 741, 815, 897, 920, 931, 1076
BshVI	ATCGAT	1 cut(s)	248
BsiEI	CGRYCG	1 cut(s)	932
BsiHKCI	CYCGRG	1 cut(s)	471
BsiSI	CCGG	4 cut(s)	636, 816, 928, 1002
BslFI	GGGAC	2 cut(s)	617, 652
BslI	CCNNNNNNNGG	2 cut(s)	572, 927
BsmAI	GTCTC	1 cut(s)	516
BsmFI	GGGAC	2 cut(s)	617, 652
BsnI	GGCC	7 cut(s)	53, 741, 815, 897, 920, 931, 1076
BsoBI	CYCGRG	1 cut(s)	471
Bsp1407I	TGTACA	1 cut(s)	826
Bsp143I	GATC	3 cut(s)	502, 838, 1054
BspACI	CCGC	4 cut(s)	168, 675, 738, 932
BspANI	GGCC	7 cut(s)	53, 741, 815, 897, 920, 931, 1076
BspCNI	CTCAG	1 cut(s)	1105
BspDI	ATCGAT	1 cut(s)	248
BspFNI	CGCG	1 cut(s)	934
BspLI	GGNNCC	2 cut(s)	209, 820
BspPI	GGATC	2 cut(s)	497, 833
BsrFI	RCCGGY	1 cut(s)	927
BsrGI	TGTACA	1 cut(s)	826
BssAI	RCCGGY	1 cut(s)	927
BssECI	CCNNGG	1 cut(s)	1071
BssMI	GATC	3 cut(s)	502, 838, 1054
BssSI	CACGAG	2 cut(s)	491, 627
BssT1I	CCWWGG	1 cut(s)	1071
Bst2BI	CACGAG	2 cut(s)	491, 627
Bst2UI	CCWGG	1 cut(s)	785
BstAPI	GCANNNNNTGC	1 cut(s)	27
BstAUI	TGTACA	1 cut(s)	826
BstC8I	GCNNGC	2 cut(s)	155, 677
BstDEI	CTNAG	2 cut(s)	197, 1113
BstF5I	GGATG	1 cut(s)	429
BstFNI	CGCG	1 cut(s)	934
BstKTI	GATC	3 cut(s)	505, 841, 1057
BstMAI	GTCTC	1 cut(s)	516
BstMBI	GATC	3 cut(s)	502, 838, 1054
BstMCI	CGRYCG	1 cut(s)	932
BstMWI	GCNNNNNNNGC	4 cut(s)	27, 174, 281, 812
BstNI	CCWGG	1 cut(s)	785
BstNSI	RCATGY	1 cut(s)	766
BstSCI	CCNGG	3 cut(s)	635, 783, 1000
BstSFI	CTRYAG	1 cut(s)	703
BstUI	CGCG	1 cut(s)	934
BstV1I	GCAGC	3 cut(s)	271, 334, 1101
BstV2I	GAAGAC	1 cut(s)	978
BstZI	CGGCCG	1 cut(s)	929
Bsu15I	ATCGAT	1 cut(s)	248
BsuRI	GGCC	7 cut(s)	53, 741, 815, 897, 920, 931, 1076
BsuTUI	ATCGAT	1 cut(s)	248
BtsCI	GGATG	1 cut(s)	429
Cac8I	GCNNGC	2 cut(s)	155, 677
CaiI	CAGNNNCTG	1 cut(s)	784
Cfr10I	RCCGGY	1 cut(s)	927
Cfr13I	GGNCC	1 cut(s)	918
ClaI	ATCGAT	1 cut(s)	248
CsiI	ACCWGGT	1 cut(s)	783
Csp6I	GTAC	2 cut(s)	781, 827
CviAII	CATG	6 cut(s)	305, 373, 763, 811, 824, 1021
CviQI	GTAC	2 cut(s)	781, 827
DdeI	CTNAG	2 cut(s)	197, 1113
DpnI	GATC	3 cut(s)	504, 840, 1056
DpnII	GATC	3 cut(s)	502, 838, 1054
EaeI	YGGCCR	3 cut(s)	739, 813, 929
EagI	CGGCCG	1 cut(s)	929
EciI	GGCGGA	1 cut(s)	183
EclXI	CGGCCG	1 cut(s)	929
Eco130I	CCWWGG	1 cut(s)	1071
Eco147I	AGGCCT	2 cut(s)	897, 1076
Eco32I	GATATC	2 cut(s)	356, 1027
Eco52I	CGGCCG	1 cut(s)	929
Eco57I	CTGAAG	2 cut(s)	79, 833
Eco88I	CYCGRG	1 cut(s)	471
EcoRII	CCWGG	1 cut(s)	783
EcoRV	GATATC	2 cut(s)	356, 1027
EcoT14I	CCWWGG	1 cut(s)	1071
ErhI	CCWWGG	1 cut(s)	1071
FaeI	CATG	6 cut(s)	308, 376, 766, 814, 827, 1024
FaqI	GGGAC	2 cut(s)	617, 652
FatI	CATG	6 cut(s)	304, 372, 762, 810, 823, 1020
FauI	CCCGC	1 cut(s)	668
FauNDI	CATATG	1 cut(s)	1051
FbaI	TGATCA	1 cut(s)	1054
FblI	GTMKAC	1 cut(s)	363
Fnu4HI	GCNGC	5 cut(s)	285, 348, 739, 932, 1090
FokI	GGATG	1 cut(s)	436
Fsp4HI	GCNGC	5 cut(s)	285, 348, 739, 932, 1090
FspBI	CTAG	3 cut(s)	150, 833, 1086
GluI	GCNGC	5 cut(s)	285, 348, 739, 932, 1090
GsuI	CTGGAG	1 cut(s)	642
HaeIII	GGCC	7 cut(s)	53, 741, 815, 897, 920, 931, 1076
HapII	CCGG	4 cut(s)	636, 816, 928, 1002
Hin1II	CATG	6 cut(s)	308, 376, 766, 814, 827, 1024
HindIII	AAGCTT	1 cut(s)	155
HinfI	GANTC	6 cut(s)	245, 310, 477, 685, 882, 983
HpaII	CCGG	4 cut(s)	636, 816, 928, 1002
HphI	GGTGA	1 cut(s)	33
Hpy166II	GTNNAC	1 cut(s)	364
Hpy188III	TCNNGA	4 cut(s)	86, 548, 659, 886
Hpy8I	GTNNAC	1 cut(s)	364
HpyAV	CCTTC	4 cut(s)	158, 787, 873, 883
HpyCH4IV	ACGT	1 cut(s)	618
HpyCH4V	TGCA	8 cut(s)	21, 70, 111, 350, 517, 668, 766, 910
HpyF10VI	GCNNNNNNNGC	4 cut(s)	27, 174, 281, 812
HpyF3I	CTNAG	2 cut(s)	197, 1113
HpySE526I	ACGT	1 cut(s)	618
Hsp92II	CATG	6 cut(s)	308, 376, 766, 814, 827, 1024
Ksp22I	TGATCA	1 cut(s)	1054
Kzo9I	GATC	3 cut(s)	502, 838, 1054
LmnI	GCTCC	4 cut(s)	207, 344, 661, 775
Lsp1109I	GCAGC	3 cut(s)	271, 334, 1101
MabI	ACCWGGT	1 cut(s)	783
MaeI	CTAG	3 cut(s)	150, 833, 1086
MaeII	ACGT	1 cut(s)	618
MalI	GATC	3 cut(s)	504, 840, 1056
MboI	GATC	3 cut(s)	502, 838, 1054
MboII	GAAGA	2 cut(s)	536, 983
MluCI	AATT	5 cut(s)	99, 278, 556, 669, 729
MlyI	GAGTC	3 cut(s)	471, 679, 977
MmeI	TCCRAC	2 cut(s)	705, 987
MseI	TTAA	2 cut(s)	528, 599
MspA1I	CMGCKG	1 cut(s)	738
MspI	CCGG	4 cut(s)	636, 816, 928, 1002
MspR9I	CCNGG	3 cut(s)	637, 785, 1002
MvaI	CCWGG	1 cut(s)	785
MvnI	CGCG	1 cut(s)	934
MwoI	GCNNNNNNNGC	4 cut(s)	27, 174, 281, 812
NciI	CCSGG	2 cut(s)	637, 1002
NdeI	CATATG	1 cut(s)	1051
NdeII	GATC	3 cut(s)	502, 838, 1054
NlaIII	CATG	6 cut(s)	308, 376, 766, 814, 827, 1024
NlaIV	GGNNCC	2 cut(s)	209, 820
NspI	RCATGY	1 cut(s)	766
PaeR7I	CTCGAG	1 cut(s)	471
PceI	AGGCCT	2 cut(s)	897, 1076
PfeI	GAWTC	3 cut(s)	245, 310, 882
PkrI	GCNGC	5 cut(s)	286, 349, 740, 933, 1091
PleI	GAGTC	3 cut(s)	471, 679, 977
PpsI	GAGTC	3 cut(s)	471, 679, 977
PsiI	TTATAA	1 cut(s)	186
Psp6I	CCWGG	1 cut(s)	783
PspGI	CCWGG	1 cut(s)	783
PspN4I	GGNNCC	2 cut(s)	209, 820
PspPI	GGNCC	1 cut(s)	918
PspXI	VCTCGAGB	1 cut(s)	471
PstNI	CAGNNNCTG	1 cut(s)	784
RsaI	GTAC	2 cut(s)	782, 828
RsaNI	GTAC	2 cut(s)	781, 827
SaqAI	TTAA	2 cut(s)	528, 599
SatI	GCNGC	5 cut(s)	285, 348, 739, 932, 1090
Sau3AI	GATC	3 cut(s)	502, 838, 1054
Sau96I	GGNCC	1 cut(s)	918
SchI	GAGTC	3 cut(s)	471, 679, 977
ScrFI	CCNGG	3 cut(s)	637, 785, 1002
SexAI	ACCWGGT	1 cut(s)	783
SfcI	CTRYAG	1 cut(s)	703
Sfr274I	CTCGAG	1 cut(s)	471
SlaI	CTCGAG	1 cut(s)	471
SmlI	CTYRAG	1 cut(s)	471
SmoI	CTYRAG	1 cut(s)	471
SpeI	ACTAGT	1 cut(s)	832
Sse9I	AATT	5 cut(s)	99, 278, 556, 669, 729
SseBI	AGGCCT	2 cut(s)	897, 1076
SsiI	CCGC	4 cut(s)	168, 675, 738, 932
SspMI	CTAG	3 cut(s)	150, 833, 1086
StuI	AGGCCT	2 cut(s)	897, 1076
StyD4I	CCNGG	3 cut(s)	635, 783, 1000
StyI	CCWWGG	1 cut(s)	1071
TaiI	ACGT	1 cut(s)	621
TaqI	TCGA	6 cut(s)	243, 248, 458, 472, 590, 688
TasI	AATT	5 cut(s)	99, 278, 556, 669, 729
TatI	WGTACW	1 cut(s)	826
TauI	GCSGC	2 cut(s)	741, 934
TfiI	GAWTC	3 cut(s)	245, 310, 882
Tru1I	TTAA	2 cut(s)	528, 599
Tru9I	TTAA	2 cut(s)	528, 599
TseI	GCWGC	3 cut(s)	284, 347, 1089
TspDTI	ATGAA	5 cut(s)	17, 259, 550, 869, 1009
TspGWI	ACGGA	1 cut(s)	223
XapI	RAATTY	2 cut(s)	99, 556
XceI	RCATGY	1 cut(s)	766
XhoI	CTCGAG	1 cut(s)	471
XmiI	GTMKAC	1 cut(s)	363
XspI	CTAG	3 cut(s)	150, 833, 1086

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.