Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000003

Orthogroup ID

OG0005919

Physical Location & Seq

Genomic Coordinates Forward (+)

8018490 .. 8020642

Gene Structure 2153 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug03G0102100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1308 bp

ATGCCTGAGATCGAGGTCGTCTCCGACGCCGCCTCAGAAGCCTCCTCGGAGGAGGGCGGCGGCGCCTTGTTCGGGAAATATGAGCTGGGGAAGCTCCTCGGCCGCGGCGCCTTTGCCAAGGTCTACCACGCGCGGGACGTCAGCAACGGGCGGAGCGTGGCGATCAAGGCGGTGAGCAAGCAGAAGGTGCTCAAGGGCGGCTTCACGGCGAACGTGAAGCGCGAGATCTCGATCATGCGGCGGCTGCAGCACCCGCACATCGTGAAGCTCTACGAGGTGTTGGCGACGAAAACCAAGATCTATTTCATCATGGAGTTCGCGAAAGGCGGCGAGTTGTTCGGGAAGATATCCAAGGGGAGGTTCAGCGAGGATCTCAGCCGCCGGTACTTCCAGCAGCTGATCTCCGCCGTCGGATACTGCCACTCCCACGGCGTCTTCCACCGCGATTTAAAGCCGGAGAATTTGCTGTTGGATGAGAATTGGAATTTGAAGGTCTCGGATTTCGGACTCTCCGCCGTGACGGAGCAGATCCGACCCGACGGGCTTCTCCACACTCTCTGCGGCACCCCCGCCTACGTGGCGCCGGAGATTCTCGAAAAGAAAGGCTACGACGGCGCCAAGGTGGACATATGGTCGTGCGGCATCATACTCTTTGTTCTCAACGCCGGTTACTTGCCGTTCAACGACCCCAATCTGATGGTAATGTACCGGAAAATTTACAAAGGAGAGTTTCGCTTTCCGAGGTGGACGTCGCCTGACCTGAAACGCTTGATTACGCGCCTGCTGGACACGAACGTCGAGACGAGGATCACCGTCGACGAGATTATCAAGGATCATTGGTTCAGTGTGGGTTACGAGGACGTGAAGTTTCACCTGGAGGACTTTAACATGAAAGAGTGGAGAGACGAGGACAGCGACACGCCGTTGAACGCCTTCGACCTGATTTCATTCTCCTCCGGCTTCGACATCTCGGGGCTGTTCCGGAAGCCGGAAATCTCCGACTGCGGGGAGCGGTTCGTGTCGGCGGAGACGCCGGAGAGGATCATCCAGAAGGTGGAGGAGGTGGCACTAGCGGAGGGCATGACGGTGATGGAGAAGAAGAGCTGGGGGGCAAAGCTGGCGGGGCAGAATGGTAATTTGATCGTCGCCATTGGGATTTACCGGTTAACGGAGAAGCTGGTGGTGGTGGAGTTGAACAAGCGGGAAAGAATAGCGGAGAATTGCCAGCAGCAAATGTGGAAAGACAAGCTGAGGCCGCAGCTGTCGTGTCTGATATACAAACCGGAAGAACAAGTCTCCGGTGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

435

Amino Acids

49.38

Weight (kDa)

6.79

Isoelectric Point (pI)

31.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	27 - 281	2.8e-73	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	28 - 277	1.9e-36	Protein tyrosine and serine/threonine kinase
Kinase-like	PF14531	123 - 269	6.7e-06	Kinase-like
NAF	PF03822	308 - 363	1.2e-19	NAF domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005919)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g15760 FvH4_7g19720 FvH4_7g19720
malus_domestica	MD01G1109700.v1.1 MD04G1098900.v1.1 MD12G1119500.v1.1
prunus_persica	Prupe.2G214600_v2.0.a1 Prupe.6G237400_v2.0.a1 Prupe.6G237400_v2.0.a1
pyrus_communis	pycom01g13680 pycom04g09390 pycom12g11850
rosa_chinensis	RchiOBHm_Chr1g0363561
rosa_laevigata	RLG00000027557
rosa_multiflora	Rmu_sc0000548.1_g000001 Rmu_sc0004871.1_g000014 Rmu_ssc0000433.1_g000008
rosa_roxburghii	Rroxscaffold_4G00293080 Rroxscaffold_6G00411830
rosa_rugosa	Rorug01G0308100 Rorug03G0102000 Rorug03G0102100
rosa_samantha	Rh1BG280300 Rh1CG296400 Rh1DG311500 Rh3DG200700
rosa_wichuraiana	Rw1G028100 Rw3G014260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	794
AatII	GACGTC	2 cut(s)	141, 752
AccB1I	GGYRCC	5 cut(s)	62, 107, 563, 580, 614
AccB7I	CCANNNNNTGG	1 cut(s)	1054
AccBSI	CCGCTC	1 cut(s)	1012
AccI	GTMKAC	2 cut(s)	123, 816
AccII	CGCG	7 cut(s)	105, 131, 133, 222, 320, 444, 778
AccIII	TCCGGA	1 cut(s)	981
AclWI	GGATC	5 cut(s)	378, 523, 815, 840, 1049
AcoI	YGGCCR	1 cut(s)	100
AcsI	RAATTY	3 cut(s)	460, 484, 714
AcyI	GRCGYC	9 cut(s)	27, 63, 108, 138, 432, 581, 615, 749, 1031
AdeI	CACNNNGTG	1 cut(s)	262
AfaI	GTAC	2 cut(s)	386, 707
AfiI	CCNNNNNNNGG	7 cut(s)	72, 133, 357, 988, 1005, 1054, 1168
AgeI	ACCGGT	1 cut(s)	1161
AgsI	TTSAA	4 cut(s)	490, 682, 928, 1195
AhdI	GACNNNNNGTC	1 cut(s)	631
AjiI	CACGTC	1 cut(s)	862
AjnI	CCWGG	1 cut(s)	873
AjuI	GAANNNNNNNTTGG	6 cut(s)	287, 319, 344, 376, 452, 484
AloI	GAACNNNNNNTCC	2 cut(s)	824, 856
AluBI	AGCT	9 cut(s)	85, 94, 268, 397, 1104, 1117, 1177, 1249, 1261
AluI	AGCT	9 cut(s)	85, 94, 268, 397, 1104, 1117, 1177, 1249, 1261
Alw21I	GWGCWC	1 cut(s)	192
Alw26I	GTCTC	6 cut(s)	25, 499, 794, 897, 1022, 1300
AlwI	GGATC	5 cut(s)	378, 523, 815, 840, 1049
AlwNI	CAGNNNCTG	1 cut(s)	397
Ama87I	CYCGRG	1 cut(s)	970
Aor13HI	TCCGGA	1 cut(s)	981
AoxI	GGCC	2 cut(s)	100, 1253
ApeKI	GCWGC	5 cut(s)	244, 247, 394, 1228, 1258
ApoI	RAATTY	3 cut(s)	460, 484, 714
AsiGI	ACCGGT	1 cut(s)	1161
Asp700I	GAANNNNTTC	1 cut(s)	932
AspLEI	GCGC	7 cut(s)	65, 110, 133, 222, 583, 617, 780
AsuHPI	GGTGA	4 cut(s)	184, 802, 863, 1099
AvaI	CYCGRG	1 cut(s)	970
BanI	GGYRCC	5 cut(s)	62, 107, 563, 580, 614
BbsI	GAAGAC	1 cut(s)	427
Bbv12I	GWGCWC	1 cut(s)	192
BbvCI	CCTCAGC	1 cut(s)	1250
BbvI	GCAGC	5 cut(s)	231, 259, 406, 1240, 1270
BccI	CCATC	2 cut(s)	691, 1084
BceAI	ACGGC	7 cut(s)	222, 392, 445, 500, 628, 661, 907
BciT130I	CCWGG	1 cut(s)	875
BciVI	GTATCC	1 cut(s)	407
BcoDI	GTCTC	6 cut(s)	25, 499, 794, 897, 1022, 1300
BfaI	CTAG	1 cut(s)	1070
BfmI	CTRYAG	1 cut(s)	245
BfoI	RGCGCY	4 cut(s)	66, 111, 584, 618
BfuI	GTATCC	1 cut(s)	407
BglI	GCCNNNNNGGC	1 cut(s)	578
BglII	AGATCT	2 cut(s)	225, 297
Bme1390I	CCNGG	1 cut(s)	875
BmeRI	GACNNNNNGTC	1 cut(s)	631
BmeT110I	CYCGRG	1 cut(s)	970
BmgBI	CACGTC	1 cut(s)	862
BmiI	GGNNCC	5 cut(s)	64, 109, 565, 582, 616
BmrFI	CCNGG	1 cut(s)	875
BmsI	GCATC	1 cut(s)	651
BpiI	GAAGAC	1 cut(s)	427
BplI	GAGNNNNNCTC	1 cut(s)	37
BpmI	CTGGAG	1 cut(s)	896
Bpu10I	CCTNAGC	1 cut(s)	1250
BpuEI	CTTGAG	1 cut(s)	176
BsaAI	YACGTR	1 cut(s)	577
BsaBI	GATNNNNATC	1 cut(s)	230
BsaHI	GRCGYC	9 cut(s)	27, 63, 108, 138, 432, 581, 615, 749, 1031
BsaI	GGTCTC	1 cut(s)	499
BsaJI	CCNNGG	8 cut(s)	45, 97, 103, 117, 351, 427, 618, 740
BsaWI	WCCGGW	5 cut(s)	708, 981, 1161, 1282, 1298
BsaXI	ACNNNNNCTCC	2 cut(s)	407, 437
Bsc4I	CCNNNNNNNGG	7 cut(s)	72, 133, 357, 988, 1005, 1054, 1168
Bse118I	RCCGGY	3 cut(s)	381, 665, 1161
Bse8I	GATNNNNATC	1 cut(s)	230
BseAI	TCCGGA	1 cut(s)	981
BseBI	CCWGG	1 cut(s)	875
BseDI	CCNNGG	8 cut(s)	45, 97, 103, 117, 351, 427, 618, 740
BseGI	GGATG	2 cut(s)	478, 1044
BseJI	GATNNNNATC	1 cut(s)	230
BseLI	CCNNNNNNNGG	7 cut(s)	72, 133, 357, 988, 1005, 1054, 1168
BseMII	CTCAG	3 cut(s)	48, 388, 1241
BseRI	GAGGAG	5 cut(s)	34, 65, 86, 943, 1073
BseX3I	CGGCCG	1 cut(s)	100
BseXI	GCAGC	5 cut(s)	231, 259, 406, 1240, 1270
BseYI	CCCAGC	2 cut(s)	85, 1104
Bsh1236I	CGCG	7 cut(s)	105, 131, 133, 222, 320, 444, 778
Bsh1285I	CGRYCG	1 cut(s)	103
BshFI	GGCC	2 cut(s)	102, 1255
BshNI	GGYRCC	5 cut(s)	62, 107, 563, 580, 614
BshTI	ACCGGT	1 cut(s)	1161
BsiEI	CGRYCG	1 cut(s)	103
BsiHKAI	GWGCWC	1 cut(s)	192
BsiHKCI	CYCGRG	1 cut(s)	970
BslFI	GGGAC	1 cut(s)	149
BslI	CCNNNNNNNGG	7 cut(s)	72, 133, 357, 988, 1005, 1054, 1168
BsmAI	GTCTC	6 cut(s)	25, 499, 794, 897, 1022, 1300
BsmBI	CGTCTC	4 cut(s)	25, 794, 897, 1022
BsmFI	GGGAC	1 cut(s)	149
BsnI	GGCC	2 cut(s)	102, 1255
Bso31I	GGTCTC	1 cut(s)	499
BsoBI	CYCGRG	1 cut(s)	970
Bsp1286I	GDGCHC	1 cut(s)	192
Bsp13I	TCCGGA	1 cut(s)	981
Bsp68I	TCGCGA	1 cut(s)	320
BspANI	GGCC	2 cut(s)	102, 1255
BspCNI	CTCAG	3 cut(s)	47, 387, 1242
BspEI	TCCGGA	1 cut(s)	981
BspFNI	CGCG	7 cut(s)	105, 131, 133, 222, 320, 444, 778
BspLI	GGNNCC	5 cut(s)	64, 109, 565, 582, 616
BspMAI	CTGCAG	1 cut(s)	249
BspPI	GGATC	5 cut(s)	378, 523, 815, 840, 1049
BspQI	GCTCTTC	1 cut(s)	1094
BspT107I	GGYRCC	5 cut(s)	62, 107, 563, 580, 614
BspTNI	GGTCTC	1 cut(s)	499
BsrBI	CCGCTC	1 cut(s)	1012
BsrFI	RCCGGY	3 cut(s)	381, 665, 1161
BssAI	RCCGGY	3 cut(s)	381, 665, 1161
BssECI	CCNNGG	8 cut(s)	45, 97, 103, 117, 351, 427, 618, 740
BssNI	GRCGYC	9 cut(s)	27, 63, 108, 138, 432, 581, 615, 749, 1031
BssT1I	CCWWGG	3 cut(s)	117, 351, 618
Bst2UI	CCWGG	1 cut(s)	875
Bst4CI	ACNGT	2 cut(s)	814, 1087
Bst6I	CTCTTC	1 cut(s)	1094
BstACI	GRCGYC	9 cut(s)	27, 63, 108, 138, 432, 581, 615, 749, 1031
BstAPI	GCANNNNNTGC	1 cut(s)	187
BstBAI	YACGTR	1 cut(s)	577
BstC8I	GCNNGC	4 cut(s)	179, 782, 1119, 1226
BstDEI	CTNAG	4 cut(s)	6, 34, 374, 1250
BstDSI	CCRYGG	2 cut(s)	103, 427
BstF5I	GGATG	2 cut(s)	478, 1044
BstFNI	CGCG	7 cut(s)	105, 131, 133, 222, 320, 444, 778
BstH2I	RGCGCY	4 cut(s)	66, 111, 584, 618
BstHHI	GCGC	7 cut(s)	65, 110, 133, 222, 583, 617, 780
BstMAI	GTCTC	6 cut(s)	25, 499, 794, 897, 1022, 1300
BstMCI	CGRYCG	1 cut(s)	103
BstNI	CCWGG	1 cut(s)	875
BstSCI	CCNGG	1 cut(s)	873
BstSFI	CTRYAG	1 cut(s)	245
BstUI	CGCG	7 cut(s)	105, 131, 133, 222, 320, 444, 778
BstV1I	GCAGC	5 cut(s)	231, 259, 406, 1240, 1270
BstV2I	GAAGAC	1 cut(s)	427
BstX2I	RGATCY	4 cut(s)	225, 297, 370, 528
BstXI	CCANNNNNNTGG	1 cut(s)	697
BstYI	RGATCY	4 cut(s)	225, 297, 370, 528
BstZI	CGGCCG	1 cut(s)	100
BsuI	GTATCC	1 cut(s)	407
BsuRI	GGCC	2 cut(s)	102, 1255
BtgI	CCRYGG	2 cut(s)	103, 427
BtrI	CACGTC	1 cut(s)	862
BtsCI	GGATG	2 cut(s)	478, 1044
BtsIMutI	CAGTG	1 cut(s)	850
BtuMI	TCGCGA	1 cut(s)	320
Cac8I	GCNNGC	4 cut(s)	179, 782, 1119, 1226
CaiI	CAGNNNCTG	1 cut(s)	397
CfoI	GCGC	7 cut(s)	65, 110, 133, 222, 583, 617, 780
Cfr10I	RCCGGY	3 cut(s)	381, 665, 1161
Cfr42I	CCGCGG	1 cut(s)	106
CseI	GACGC	3 cut(s)	35, 421, 1039
Csp6I	GTAC	2 cut(s)	385, 706
CspAI	ACCGGT	1 cut(s)	1161
CviAII	CATG	4 cut(s)	235, 310, 889, 1081
CviQI	GTAC	2 cut(s)	385, 706
DdeI	CTNAG	4 cut(s)	6, 34, 374, 1250
DinI	GGCGCC	4 cut(s)	64, 109, 582, 616
DraI	TTTAAA	1 cut(s)	450
DraIII	CACNNNGTG	1 cut(s)	262
DrdI	GACNNNNNNGTC	1 cut(s)	794
DriI	GACNNNNNGTC	1 cut(s)	631
DseDI	GACNNNNNNGTC	1 cut(s)	794
EaeI	YGGCCR	1 cut(s)	100
EagI	CGGCCG	1 cut(s)	100
Eam1104I	CTCTTC	1 cut(s)	1094
Eam1105I	GACNNNNNGTC	1 cut(s)	631
EarI	CTCTTC	1 cut(s)	1094
EciI	GGCGGA	4 cut(s)	166, 394, 502, 1040
EclXI	CGGCCG	1 cut(s)	100
Eco130I	CCWWGG	3 cut(s)	117, 351, 618
Eco31I	GGTCTC	1 cut(s)	499
Eco32I	GATATC	1 cut(s)	348
Eco52I	CGGCCG	1 cut(s)	100
Eco88I	CYCGRG	1 cut(s)	970
EcoRII	CCWGG	1 cut(s)	873
EcoRV	GATATC	1 cut(s)	348
EcoT14I	CCWWGG	3 cut(s)	117, 351, 618
EgeI	GGCGCC	4 cut(s)	64, 109, 582, 616
EheI	GGCGCC	4 cut(s)	64, 109, 582, 616
ErhI	CCWWGG	3 cut(s)	117, 351, 618
Esp3I	CGTCTC	4 cut(s)	25, 794, 897, 1022
FaeI	CATG	4 cut(s)	238, 313, 892, 1084
FaiI	YATR	9 cut(s)	81, 236, 311, 629, 631, 647, 890, 1082, 1276
FalI	AAGNNNNNCTT	2 cut(s)	185, 217
FaqI	GGGAC	1 cut(s)	149
FatI	CATG	4 cut(s)	234, 309, 888, 1080
FauI	CCCGC	6 cut(s)	126, 261, 577, 998, 1114, 1194
FauNDI	CATATG	1 cut(s)	629
FblI	GTMKAC	2 cut(s)	123, 816
FokI	GGATG	2 cut(s)	485, 1031
FspBI	CTAG	1 cut(s)	1070
GlaI	GCGC	7 cut(s)	64, 109, 132, 221, 582, 616, 779
GsaI	CCCAGC	2 cut(s)	89, 1108
GsuI	CTGGAG	1 cut(s)	896
HaeII	RGCGCY	4 cut(s)	66, 111, 584, 618
HaeIII	GGCC	2 cut(s)	102, 1255
HgaI	GACGC	3 cut(s)	35, 421, 1039
HhaI	GCGC	7 cut(s)	65, 110, 133, 222, 583, 617, 780
Hin1I	GRCGYC	9 cut(s)	27, 63, 108, 138, 432, 581, 615, 749, 1031
Hin1II	CATG	4 cut(s)	238, 313, 892, 1084
Hin6I	GCGC	7 cut(s)	63, 108, 131, 220, 581, 615, 778
HinP1I	GCGC	7 cut(s)	63, 108, 131, 220, 581, 615, 778
HincII	GTYRAC	2 cut(s)	817, 1167
HindII	GTYRAC	2 cut(s)	817, 1167
HinfI	GANTC	2 cut(s)	507, 589
HpaI	GTTAAC	1 cut(s)	1167
HphI	GGTGA	4 cut(s)	184, 802, 863, 1099
Hpy166II	GTNNAC	5 cut(s)	124, 625, 747, 817, 1167
Hpy188III	TCNNGA	9 cut(s)	73, 229, 262, 319, 340, 593, 799, 982, 1048
Hpy8I	GTNNAC	5 cut(s)	124, 625, 747, 817, 1167
HpyAV	CCTTC	4 cut(s)	178, 484, 943, 1045
HpyCH4III	ACNGT	2 cut(s)	814, 1087
HpyCH4IV	ACGT	6 cut(s)	138, 213, 576, 749, 795, 861
HpyCH4V	TGCA	1 cut(s)	247
HpyF3I	CTNAG	4 cut(s)	6, 34, 374, 1250
HpySE526I	ACGT	6 cut(s)	138, 213, 576, 749, 795, 861
Hsp92I	GRCGYC	9 cut(s)	27, 63, 108, 138, 432, 581, 615, 749, 1031
Hsp92II	CATG	4 cut(s)	238, 313, 892, 1084
HspAI	GCGC	7 cut(s)	63, 108, 131, 220, 581, 615, 778
KasI	GGCGCC	4 cut(s)	62, 107, 580, 614
Kpn2I	TCCGGA	1 cut(s)	981
KspAI	GTTAAC	1 cut(s)	1167
KspI	CCGCGG	1 cut(s)	106
LguI	GCTCTTC	1 cut(s)	1094
LmnI	GCTCC	4 cut(s)	99, 153, 523, 1009
Lsp1109I	GCAGC	5 cut(s)	231, 259, 406, 1240, 1270
LweI	GCATC	1 cut(s)	651
MaeI	CTAG	1 cut(s)	1070
MaeII	ACGT	6 cut(s)	138, 213, 576, 749, 795, 861
MaeIII	GTNAC	3 cut(s)	517, 668, 851
MbiI	CCGCTC	1 cut(s)	1012
MboII	GAAGA	5 cut(s)	355, 427, 1108, 1111, 1298
MflI	RGATCY	4 cut(s)	225, 297, 370, 528
MhlI	GDGCHC	1 cut(s)	192
MluCI	AATT	6 cut(s)	460, 478, 484, 714, 1135, 1219
Mly113I	GGCGCC	4 cut(s)	63, 108, 581, 615
MlyI	GAGTC	1 cut(s)	501
MmeI	TCCRAC	5 cut(s)	48, 391, 450, 556, 1023
MroI	TCCGGA	1 cut(s)	981
MroXI	GAANNNNTTC	1 cut(s)	932
MseI	TTAA	3 cut(s)	449, 885, 1166
MslI	CAYNNNNRTG	1 cut(s)	1085
MspA1I	CMGCKG	3 cut(s)	105, 397, 1261
MspR9I	CCNGG	1 cut(s)	875
MvaI	CCWGG	1 cut(s)	875
MvnI	CGCG	7 cut(s)	105, 131, 133, 222, 320, 444, 778
NarI	GGCGCC	4 cut(s)	63, 108, 581, 615
NdeI	CATATG	1 cut(s)	629
NlaIII	CATG	4 cut(s)	238, 313, 892, 1084
NlaIV	GGNNCC	5 cut(s)	64, 109, 565, 582, 616
NmeAIII	GCCGAG	1 cut(s)	78
NmuCI	GTSAC	1 cut(s)	517
NruI	TCGCGA	1 cut(s)	320
PciSI	GCTCTTC	1 cut(s)	1094
PcsI	WCGNNNNNNNCGW	4 cut(s)	135, 153, 219, 912
PdmI	GAANNNNTTC	1 cut(s)	932
PfeI	GAWTC	1 cut(s)	589
PflMI	CCANNNNNTGG	1 cut(s)	1054
PinAI	ACCGGT	1 cut(s)	1161
PleI	GAGTC	1 cut(s)	501
PluTI	GGCGCC	4 cut(s)	66, 111, 584, 618
PpsI	GAGTC	1 cut(s)	501
Ppu21I	YACGTR	1 cut(s)	577
Psp6I	CCWGG	1 cut(s)	873
PspFI	CCCAGC	2 cut(s)	85, 1104
PspGI	CCWGG	1 cut(s)	873
PspN4I	GGNNCC	5 cut(s)	64, 109, 565, 582, 616
PstI	CTGCAG	1 cut(s)	249
PstNI	CAGNNNCTG	1 cut(s)	397
PsuI	RGATCY	4 cut(s)	225, 297, 370, 528
PvuII	CAGCTG	2 cut(s)	397, 1261
RruI	TCGCGA	1 cut(s)	320
RsaI	GTAC	2 cut(s)	386, 707
RsaNI	GTAC	2 cut(s)	385, 706
RseI	CAYNNNNRTG	1 cut(s)	1085
SacII	CCGCGG	1 cut(s)	106
SalI	GTCGAC	1 cut(s)	815
SapI	GCTCTTC	1 cut(s)	1094
SaqAI	TTAA	3 cut(s)	449, 885, 1166
SchI	GAGTC	1 cut(s)	501
ScrFI	CCNGG	1 cut(s)	875
SduI	GDGCHC	1 cut(s)	192
SfaNI	GCATC	1 cut(s)	651
SfcI	CTRYAG	1 cut(s)	245
SfoI	GGCGCC	4 cut(s)	64, 109, 582, 616
Sfr303I	CCGCGG	1 cut(s)	106
SgrBI	CCGCGG	1 cut(s)	106
SgrDI	CGTCGACG	1 cut(s)	815
SmiMI	CAYNNNNRTG	1 cut(s)	1085
SmlI	CTYRAG	1 cut(s)	191
SmoI	CTYRAG	1 cut(s)	191
Sse9I	AATT	6 cut(s)	460, 478, 484, 714, 1135, 1219
SspDI	GGCGCC	4 cut(s)	62, 107, 580, 614
SspMI	CTAG	1 cut(s)	1070
StyD4I	CCNGG	1 cut(s)	873
StyI	CCWWGG	3 cut(s)	117, 351, 618
TaaI	ACNGT	2 cut(s)	814, 1087
TaiI	ACGT	6 cut(s)	141, 216, 579, 752, 798, 864
TaqI	TCGA	7 cut(s)	12, 230, 594, 798, 816, 936, 963
TasI	AATT	6 cut(s)	460, 478, 484, 714, 1135, 1219
TfiI	GAWTC	1 cut(s)	589
Tru1I	TTAA	3 cut(s)	449, 885, 1166
Tru9I	TTAA	3 cut(s)	449, 885, 1166
TscAI	CASTG	1 cut(s)	850
TseFI	GTSAC	1 cut(s)	517
TseI	GCWGC	5 cut(s)	244, 247, 394, 1228, 1258
Tsp45I	GTSAC	1 cut(s)	517
TspDTI	ATGAA	3 cut(s)	295, 905, 936
TspGWI	ACGGA	2 cut(s)	536, 1184
TspRI	CASTG	1 cut(s)	850
Van91I	CCANNNNNTGG	1 cut(s)	1054
XapI	RAATTY	3 cut(s)	460, 484, 714
XmiI	GTMKAC	2 cut(s)	123, 816
XmnI	GAANNNNTTC	1 cut(s)	932
XspI	CTAG	1 cut(s)	1070
ZraI	GACGTC	2 cut(s)	139, 750

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.