Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000003

Orthogroup ID

OG0005919

Physical Location & Seq

Genomic Coordinates Reverse (-)

7988328 .. 7991220

Gene Structure 2893 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug03G0102000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2040 bp

ATGGCGGTTTCTCCACAAGCATTCCCGCCCAGAAAGCGGCGGCCATCTGCCGGAGCCTTCATGTCTCCCAAACTCTCCGACCAGAGCCTCCTCCGCTCTCTCCTCCACCTCTGCCAGGAAATCTCCTCCCTCGAATCCCGCAGCTTCCTCCTCAGGCGTACCACCCTATCCATGACTCGAAAATCCAAGCTTCTTGCCTTCCTCTTCGAAGACCTAGTTCGATCATCAATCACCTACCTCTCTCCCTCCGCTCTCTTATGTTTCCAAGAAATGTACATACTGCTGCAGAGAATCCAAACCCTCATCGAAGACTCTTCTCACGGCAGCAAGATGTGGCTTCTCATCCAAAACGAGTCCCTCGCCAACAGCTTCCACGAGCTGACTCTTGATCTATCCACTTTGCTCGATATTCTCCCCGTCAAGGAGCTTAACCTAAACGACGACGTCGAGGAAGTGGTCGCCTTGCTCCGTGCACAGTGCGCGCGAAGCAAGGACGCTTTTGTTGATCCCAAGGATGATAATCTCCGCCTCGAAGTCTGCTCCATGCTCGATCGCCTCAAAAAGGAAATCATTCCAGACCACTCCAAGCTCTCCGAGATTTTTGTCCAGTTAGGAATTCTAGACTCTTCCAAATGTAAAGAAGAGCTAGAGTCTCTCCAAGACGAGTTTCAGAACCAAGCCGACGATAAGTCCAAATCCCAATTGGTTTCCCTCATCGGCCTCGTTCGCTACGCCAAATGTGTCCTCTTCGGCGCTTCAACTCCAAGTTCCGATCTCACTCGCCTGAAATCTTCCTCGGAGCTAAACTTCCCGGCGGACTTCCGGTGTCCGATCAGCTTAGAGCTTATGCGGGACCCGGTCGCCGTGGCCACCGGTCAGACATACGATCGTGAGTCCATCAAGCTGTGGATCGAATCAGGACACAACACGTGTCCCAAGACAGGTCAGACGCTGGCCCACACGAACTTGATACCCAACCGCGCTTTGAAAAATCTTATCGCCATGTGGTGCCGCGAGCAGCGGATCCCTTTCGAGCACGCCGAGAATAAGGATAAAGTCAGCGCCGTCAAGAAGAACAAGGCGGCGTTAGAAGCCACCAGGATGACGGCGTCGTTTTTGGTCAACAAACTCTCCCTCGCCCAGTCAATCGAGGCTGCCAACGGCGTCGTTTACGAGCTTCGTAGCCTGGCCAAGACTGATTCGGACTGCCGAGCCTGCATTGCCGAAGCTGGAGCGATTCCTTTGCTCGTACGTCATTTAGGCTCGGACGTCGGTCGGGACCACCCGAACCTACAGGTGAACGCCGTCACGACGATCCTCAACCTCTCAATACTCGAAGCCAACAAAGCAAGGATCATGGACACTGACGGAGCCTTAAACGGCGTCATCGAGGTCCTTCGTTCGGGTGCCACATGGGAGGCTAAAGGGAACGCGGCGGCTACGATATTCAGCTTATCAGGTGTGCACGCGTACAGGAAGAAACTAGGGAGGAAGGCACGTGTCATAAAGGCATTGGTGGACTTGGCCAGGGAGGGACCCGGGTTTTCCAAAAGGGACGCCATGGTGGCGATATTGAATTTGGCGGGAGAAAGAGAGGCGGTGGCGAAGTTGGTGGAAGGCGGGGTGGTGGAGATGACGATGGAAGTAATGGAGGGGCTACCGGAAGAGGCGGTGACGGTGTTGGAAGTGGTGGTGAGAGGAGGAGGGATGGGGGCAATTGCGGCGGCGTATGGGGCCATCAGGCATTTGGGAGTGGTGTTGAGAGAAGGGTCGGATAGGGCGAAGGAGAGCGCGGCGGCGACACTTGTGACAATTTGTAGAAAAGGGGGGTCGGAAATGGTGGTGGAGTTGGTGTCGGTTTCGGGGATAGAGAGGGTAATATGGGAATTGATGGGGACGGGGACGGTGAGGGCGAGGAGGAAAGCGGCGTCGTTGTTGAGGATTCTGAGGAGATGGGCGGCCGGATTGGAGGGTGATCAAGTAGTGGAAGGGTATTCGACTATTGTGAGTTCATCAACGACAGCAATTGTGTCTAGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

679

Amino Acids

74.28

Weight (kDa)

8.33

Isoelectric Point (pI)

43.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PUB10_N	PF25368	52 - 164	1.1e-10	PUB10-like, N-terminal domain
U-box	PF04564	271 - 341	2e-20	U-box domain
ARM_PUB	PF25598	377 - 651	4.9e-14	Plant U-box protein ARM repeat region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005919)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g15760 FvH4_7g19720 FvH4_7g19720
malus_domestica	MD01G1109700.v1.1 MD04G1098900.v1.1 MD12G1119500.v1.1
prunus_persica	Prupe.2G214600_v2.0.a1 Prupe.6G237400_v2.0.a1 Prupe.6G237400_v2.0.a1
pyrus_communis	pycom01g13680 pycom04g09390 pycom12g11850
rosa_chinensis	RchiOBHm_Chr1g0363561
rosa_laevigata	RLG00000027557
rosa_multiflora	Rmu_sc0000548.1_g000001 Rmu_sc0004871.1_g000014 Rmu_ssc0000433.1_g000008
rosa_roxburghii	Rroxscaffold_4G00293080 Rroxscaffold_6G00411830
rosa_rugosa	Rorug01G0308100 Rorug03G0102000 Rorug03G0102100
rosa_samantha	Rh1BG280300 Rh1CG296400 Rh1DG311500 Rh3DG200700
rosa_wichuraiana	Rw1G028100 Rw3G014260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	447, 1272
AccB1I	GGYRCC	2 cut(s)	1008, 1406
AccBSI	CCGCTC	2 cut(s)	96, 251
AccII	CGCG	7 cut(s)	482, 484, 981, 1014, 1433, 1469, 1793
AclWI	GGATC	6 cut(s)	500, 917, 1018, 1031, 1309, 1361
AcoI	YGGCCR	5 cut(s)	41, 867, 1188, 1524, 1959
AcsI	RAATTY	2 cut(s)	615, 1576
AcvI	CACGTG	2 cut(s)	930, 1499
AcyI	GRCGYC	7 cut(s)	444, 1109, 1164, 1269, 1383, 1557, 1928
AfaI	GTAC	4 cut(s)	160, 275, 1251, 1472
AfiI	CCNNNNNNNGG	7 cut(s)	36, 50, 115, 421, 562, 941, 1402
AflIII	ACRYGT	4 cut(s)	927, 929, 1467, 1498
AgeI	ACCGGT	1 cut(s)	872
AgsI	TTSAA	3 cut(s)	759, 988, 1576
AhdI	GACNNNNNGTC	1 cut(s)	688
AjnI	CCWGG	4 cut(s)	114, 1097, 1185, 1526
AjuI	GAANNNNNNNTTGG	2 cut(s)	651, 683
Alw21I	GWGCWC	3 cut(s)	475, 1038, 1467
Alw26I	GTCTC	2 cut(s)	69, 657
Alw44I	GTGCAC	2 cut(s)	471, 1463
AlwI	GGATC	6 cut(s)	500, 917, 1018, 1031, 1309, 1361
AlwNI	CAGNNNCTG	1 cut(s)	952
Ama87I	CYCGRG	1 cut(s)	1538
AoxI	GGCC	8 cut(s)	41, 718, 867, 954, 1188, 1524, 1734, 1959
ApaLI	GTGCAC	2 cut(s)	471, 1463
ApeKI	GCWGC	5 cut(s)	141, 283, 324, 1018, 1154
ApoI	RAATTY	2 cut(s)	615, 1576
AsiGI	ACCGGT	1 cut(s)	872
Asp700I	GAANNNNTTC	1 cut(s)	570
AspLEI	GCGC	6 cut(s)	482, 484, 755, 983, 1064, 1793
AspS9I	GGNCC	6 cut(s)	853, 955, 1279, 1393, 1535, 1734
AsuC2I	CCSGG	4 cut(s)	812, 857, 1539, 1540
AsuHPI	GGTGA	6 cut(s)	223, 1309, 1684, 1705, 1918, 1985
AsuII	TTCGAA	1 cut(s)	207
AvaI	CYCGRG	1 cut(s)	1538
AvaII	GGWCC	4 cut(s)	853, 1279, 1393, 1535
AxyI	CCTNAGG	1 cut(s)	152
BaeGI	GKGCMC	2 cut(s)	475, 1467
BalI	TGGCCA	3 cut(s)	869, 1190, 1526
BamHI	GGATCC	1 cut(s)	1023
BanI	GGYRCC	2 cut(s)	1008, 1406
BauI	CACGAG	1 cut(s)	374
BbrPI	CACGTG	2 cut(s)	930, 1499
BbsI	GAAGAC	2 cut(s)	216, 315
Bbv12I	GWGCWC	3 cut(s)	475, 1038, 1467
BbvI	GCAGC	5 cut(s)	153, 270, 336, 1030, 1141
BccI	CCATC	7 cut(s)	52, 905, 1633, 1702, 1745, 1885, 1947
BceAI	ACGGC	7 cut(s)	337, 848, 1049, 1122, 1177, 1289, 1396
BcgI	CGANNNNNNTGC	2 cut(s)	1225, 1259
BciT130I	CCWGG	4 cut(s)	116, 1099, 1187, 1528
BclI	TGATCA	1 cut(s)	1975
BcnI	CCSGG	4 cut(s)	812, 857, 1539, 1540
BcoDI	GTCTC	2 cut(s)	69, 657
BfaI	CTAG	5 cut(s)	215, 620, 647, 1484, 2034
BfmI	CTRYAG	2 cut(s)	284, 1292
BfoI	RGCGCY	2 cut(s)	756, 1065
BglI	GCCNNNNNGGC	1 cut(s)	1565
Bme1390I	CCNGG	8 cut(s)	116, 812, 857, 1099, 1187, 1528, 1539, 1540
Bme18I	GGWCC	4 cut(s)	853, 1279, 1393, 1535
BmeRI	GACNNNNNGTC	1 cut(s)	688
BmeT110I	CYCGRG	1 cut(s)	1538
BmgT120I	GGNCC	6 cut(s)	853, 955, 1279, 1393, 1535, 1734
BmrFI	CCNGG	8 cut(s)	116, 812, 857, 1099, 1187, 1528, 1539, 1540
BmrI	ACTGGG	1 cut(s)	1135
BmuI	ACTGGG	1 cut(s)	1135
BoxI	GACNNNNGTC	1 cut(s)	1272
BpiI	GAAGAC	2 cut(s)	216, 315
BpmI	CTGGAG	1 cut(s)	1251
Bpu14I	TTCGAA	1 cut(s)	207
BpuMI	CCSGG	4 cut(s)	812, 857, 1539, 1540
BsaAI	YACGTR	2 cut(s)	930, 1499
BsaBI	GATNNNNATC	1 cut(s)	519
BsaHI	GRCGYC	7 cut(s)	444, 1109, 1164, 1269, 1383, 1557, 1928
BsaJI	CCNNGG	6 cut(s)	510, 795, 864, 1527, 1538, 1560
BsaWI	WCCGGW	3 cut(s)	822, 872, 1660
BsaXI	ACNNNNNCTCC	4 cut(s)	1115, 1145, 1838, 1868
Bsc4I	CCNNNNNNNGG	7 cut(s)	36, 50, 115, 421, 562, 941, 1402
Bse118I	RCCGGY	1 cut(s)	872
Bse1I	ACTGG	2 cut(s)	607, 1141
Bse21I	CCTNAGG	1 cut(s)	152
Bse3DI	GCAATG	1 cut(s)	1218
Bse8I	GATNNNNATC	1 cut(s)	519
BseBI	CCWGG	4 cut(s)	116, 1099, 1187, 1528
BseDI	CCNNGG	6 cut(s)	510, 795, 864, 1527, 1538, 1560
BseGI	GGATG	4 cut(s)	342, 520, 1107, 1713
BseJI	GATNNNNATC	1 cut(s)	519
BseLI	CCNNNNNNNGG	7 cut(s)	36, 50, 115, 421, 562, 941, 1402
BseMI	GCAATG	1 cut(s)	1218
BseMII	CTCAG	2 cut(s)	166, 1937
BseNI	ACTGG	2 cut(s)	607, 1141
BsePI	GCGCGC	1 cut(s)	480
BseRI	GAGGAG	8 cut(s)	80, 92, 115, 140, 1713, 1716, 1930, 1963
BseSI	GKGCMC	2 cut(s)	475, 1467
BseX3I	CGGCCG	1 cut(s)	1959
BseXI	GCAGC	5 cut(s)	153, 270, 336, 1030, 1141
Bsh1236I	CGCG	7 cut(s)	482, 484, 981, 1014, 1433, 1469, 1793
Bsh1285I	CGRYCG	5 cut(s)	553, 861, 889, 1276, 1962
BshFI	GGCC	8 cut(s)	43, 720, 869, 956, 1190, 1526, 1736, 1961
BshNI	GGYRCC	2 cut(s)	1008, 1406
BshTI	ACCGGT	1 cut(s)	872
BsiEI	CGRYCG	5 cut(s)	553, 861, 889, 1276, 1962
BsiHKAI	GWGCWC	3 cut(s)	475, 1038, 1467
BsiHKCI	CYCGRG	1 cut(s)	1538
BsiSI	CCGG	8 cut(s)	51, 812, 823, 857, 873, 1539, 1661, 1962
BsiWI	CGTACG	1 cut(s)	1249
BslFI	GGGAC	8 cut(s)	340, 866, 918, 1292, 1548, 1568, 1909, 1915
BslI	CCNNNNNNNGG	7 cut(s)	36, 50, 115, 421, 562, 941, 1402
BsmAI	GTCTC	2 cut(s)	69, 657
BsmFI	GGGAC	8 cut(s)	340, 866, 918, 1292, 1548, 1568, 1909, 1915
BsmI	GAATGC	1 cut(s)	20
BsnI	GGCC	8 cut(s)	43, 720, 869, 956, 1190, 1526, 1736, 1961
BsoBI	CYCGRG	1 cut(s)	1538
Bsp119I	TTCGAA	1 cut(s)	207
Bsp1286I	GDGCHC	3 cut(s)	475, 1038, 1467
Bsp1407I	TGTACA	1 cut(s)	273
Bsp19I	CCATGG	1 cut(s)	1560
BspANI	GGCC	8 cut(s)	43, 720, 869, 956, 1190, 1526, 1736, 1961
BspCNI	CTCAG	2 cut(s)	165, 1938
BspFNI	CGCG	7 cut(s)	482, 484, 981, 1014, 1433, 1469, 1793
BspMAI	CTGCAG	1 cut(s)	288
BspPI	GGATC	6 cut(s)	500, 917, 1018, 1031, 1309, 1361
BspQI	GCTCTTC	1 cut(s)	636
BspT104I	TTCGAA	1 cut(s)	207
BspT107I	GGYRCC	2 cut(s)	1008, 1406
BsrBI	CCGCTC	2 cut(s)	96, 251
BsrDI	GCAATG	1 cut(s)	1218
BsrFI	RCCGGY	1 cut(s)	872
BsrGI	TGTACA	1 cut(s)	273
BsrI	ACTGG	2 cut(s)	607, 1141
BssAI	RCCGGY	1 cut(s)	872
BssECI	CCNNGG	6 cut(s)	510, 795, 864, 1527, 1538, 1560
BssHII	GCGCGC	1 cut(s)	480
BssNI	GRCGYC	7 cut(s)	444, 1109, 1164, 1269, 1383, 1557, 1928
BssSI	CACGAG	1 cut(s)	374
BssT1I	CCWWGG	2 cut(s)	510, 1560
Bst2BI	CACGAG	1 cut(s)	374
Bst2UI	CCWGG	4 cut(s)	116, 1099, 1187, 1528
Bst4CI	ACNGT	3 cut(s)	477, 1678, 1906
Bst6I	CTCTTC	6 cut(s)	209, 319, 631, 636, 752, 1659
BstACI	GRCGYC	7 cut(s)	444, 1109, 1164, 1269, 1383, 1557, 1928
BstAUI	TGTACA	1 cut(s)	273
BstBAI	YACGTR	2 cut(s)	930, 1499
BstBI	TTCGAA	1 cut(s)	207
BstC8I	GCNNGC	6 cut(s)	482, 954, 1016, 1038, 1216, 1467
BstDEI	CTNAG	3 cut(s)	152, 838, 1946
BstDSI	CCRYGG	2 cut(s)	864, 1560
BstENI	CCTNNNNNAGG	2 cut(s)	113, 560
BstF5I	GGATG	4 cut(s)	342, 520, 1107, 1713
BstFNI	CGCG	7 cut(s)	482, 484, 981, 1014, 1433, 1469, 1793
BstH2I	RGCGCY	2 cut(s)	756, 1065
BstHHI	GCGC	6 cut(s)	482, 484, 755, 983, 1064, 1793
BstMAI	GTCTC	2 cut(s)	69, 657
BstMCI	CGRYCG	5 cut(s)	553, 861, 889, 1276, 1962
BstNI	CCWGG	4 cut(s)	116, 1099, 1187, 1528
BstPAI	GACNNNNGTC	1 cut(s)	1272
BstSCI	CCNGG	8 cut(s)	114, 810, 855, 1097, 1185, 1526, 1537, 1538
BstSFI	CTRYAG	2 cut(s)	284, 1292
BstSLI	GKGCMC	2 cut(s)	475, 1467
BstUI	CGCG	7 cut(s)	482, 484, 981, 1014, 1433, 1469, 1793
BstV1I	GCAGC	5 cut(s)	153, 270, 336, 1030, 1141
BstV2I	GAAGAC	2 cut(s)	216, 315
BstX2I	RGATCY	1 cut(s)	1023
BstYI	RGATCY	1 cut(s)	1023
BstZI	CGGCCG	1 cut(s)	1959
Bsu36I	CCTNAGG	1 cut(s)	152
BsuRI	GGCC	8 cut(s)	43, 720, 869, 956, 1190, 1526, 1736, 1961
BtgI	CCRYGG	2 cut(s)	864, 1560
BtsCI	GGATG	4 cut(s)	342, 520, 1107, 1713
BtsIMutI	CAGTG	2 cut(s)	482, 1362
Cac8I	GCNNGC	6 cut(s)	482, 954, 1016, 1038, 1216, 1467
CaiI	CAGNNNCTG	1 cut(s)	952
CfoI	GCGC	6 cut(s)	482, 484, 755, 983, 1064, 1793
Cfr10I	RCCGGY	1 cut(s)	872
Cfr13I	GGNCC	6 cut(s)	853, 955, 1279, 1393, 1535, 1734
Cfr9I	CCCGGG	1 cut(s)	1538
CseI	GACGC	7 cut(s)	503, 958, 1098, 1153, 1372, 1565, 1917
Csp6I	GTAC	4 cut(s)	159, 274, 1250, 1471
CspAI	ACCGGT	1 cut(s)	872
CviAII	CATG	7 cut(s)	61, 172, 544, 1003, 1357, 1413, 1561
CviQI	GTAC	4 cut(s)	159, 274, 1250, 1471
DdeI	CTNAG	3 cut(s)	152, 838, 1946
DriI	GACNNNNNGTC	1 cut(s)	688
EaeI	YGGCCR	5 cut(s)	41, 867, 1188, 1524, 1959
EagI	CGGCCG	1 cut(s)	1959
Eam1104I	CTCTTC	6 cut(s)	209, 319, 631, 636, 752, 1659
Eam1105I	GACNNNNNGTC	1 cut(s)	688
EarI	CTCTTC	6 cut(s)	209, 319, 631, 636, 752, 1659
EciI	GGCGGA	2 cut(s)	515, 830
EclXI	CGGCCG	1 cut(s)	1959
Eco130I	CCWWGG	2 cut(s)	510, 1560
Eco47I	GGWCC	4 cut(s)	853, 1279, 1393, 1535
Eco52I	CGGCCG	1 cut(s)	1959
Eco72I	CACGTG	2 cut(s)	930, 1499
Eco81I	CCTNAGG	1 cut(s)	152
Eco88I	CYCGRG	1 cut(s)	1538
EcoNI	CCTNNNNNAGG	2 cut(s)	113, 560
EcoO109I	RGGNCCY	3 cut(s)	853, 1393, 1535
EcoRI	GAATTC	1 cut(s)	615
EcoRII	CCWGG	4 cut(s)	114, 1097, 1185, 1526
EcoT14I	CCWWGG	2 cut(s)	510, 1560
ErhI	CCWWGG	2 cut(s)	510, 1560
FaeI	CATG	7 cut(s)	64, 175, 547, 1006, 1360, 1416, 1564
FaqI	GGGAC	8 cut(s)	340, 866, 918, 1292, 1548, 1568, 1909, 1915
FatI	CATG	7 cut(s)	60, 171, 543, 1002, 1356, 1412, 1560
FauI	CCCGC	5 cut(s)	33, 146, 843, 1576, 1613
FbaI	TGATCA	1 cut(s)	1975
FokI	GGATG	4 cut(s)	329, 527, 1114, 1720
FspBI	CTAG	5 cut(s)	215, 620, 647, 1484, 2034
GlaI	GCGC	6 cut(s)	481, 483, 754, 982, 1063, 1792
GsuI	CTGGAG	1 cut(s)	1251
HaeII	RGCGCY	2 cut(s)	756, 1065
HaeIII	GGCC	8 cut(s)	43, 720, 869, 956, 1190, 1526, 1736, 1961
HapII	CCGG	8 cut(s)	51, 812, 823, 857, 873, 1539, 1661, 1962
HgaI	GACGC	7 cut(s)	503, 958, 1098, 1153, 1372, 1565, 1917
HhaI	GCGC	6 cut(s)	482, 484, 755, 983, 1064, 1793
Hin1I	GRCGYC	7 cut(s)	444, 1109, 1164, 1269, 1383, 1557, 1928
Hin1II	CATG	7 cut(s)	64, 175, 547, 1006, 1360, 1416, 1564
Hin6I	GCGC	6 cut(s)	480, 482, 753, 981, 1062, 1791
HinP1I	GCGC	6 cut(s)	480, 482, 753, 981, 1062, 1791
HincII	GTYRAC	1 cut(s)	1123
HindII	GTYRAC	1 cut(s)	1123
HindIII	AAGCTT	1 cut(s)	188
HpaII	CCGG	8 cut(s)	51, 812, 823, 857, 873, 1539, 1661, 1962
HphI	GGTGA	6 cut(s)	223, 1309, 1684, 1705, 1918, 1985
Hpy166II	GTNNAC	6 cut(s)	473, 1123, 1171, 1300, 1465, 1519
Hpy188III	TCNNGA	8 cut(s)	386, 575, 620, 890, 918, 1069, 1277, 1309
Hpy8I	GTNNAC	6 cut(s)	473, 1123, 1171, 1300, 1465, 1519
Hpy99I	CGWCG	9 cut(s)	443, 446, 449, 686, 1114, 1169, 1274, 1315, 1933
HpyAV	CCTTC	8 cut(s)	67, 208, 1406, 1486, 1610, 1760, 1777, 1982
HpyCH4III	ACNGT	3 cut(s)	477, 1678, 1906
HpyCH4IV	ACGT	5 cut(s)	444, 929, 1252, 1269, 1498
HpyCH4V	TGCA	4 cut(s)	286, 473, 1218, 1465
HpyF3I	CTNAG	3 cut(s)	152, 838, 1946
HpySE526I	ACGT	5 cut(s)	444, 929, 1252, 1269, 1498
Hsp92I	GRCGYC	7 cut(s)	444, 1109, 1164, 1269, 1383, 1557, 1928
Hsp92II	CATG	7 cut(s)	64, 175, 547, 1006, 1360, 1416, 1564
HspAI	GCGC	6 cut(s)	480, 482, 753, 981, 1062, 1791
KflI	GGGWCCC	2 cut(s)	853, 1535
Ksp22I	TGATCA	1 cut(s)	1975
LguI	GCTCTTC	1 cut(s)	636
LmnI	GCTCC	7 cut(s)	53, 424, 471, 545, 799, 1232, 1370
Lsp1109I	GCAGC	5 cut(s)	153, 270, 336, 1030, 1141
MaeI	CTAG	5 cut(s)	215, 620, 647, 1484, 2034
MaeII	ACGT	5 cut(s)	444, 929, 1252, 1269, 1498
MaeIII	GTNAC	3 cut(s)	1306, 1672, 1807
MbiI	CCGCTC	2 cut(s)	96, 251
MfeI	CAATTG	3 cut(s)	701, 1716, 2025
MflI	RGATCY	1 cut(s)	1023
MhlI	GDGCHC	3 cut(s)	475, 1038, 1467
MlsI	TGGCCA	3 cut(s)	869, 1190, 1526
MluCI	AATT	7 cut(s)	615, 701, 1576, 1716, 1812, 1886, 2025
MluI	ACGCGT	1 cut(s)	1467
MluNI	TGGCCA	3 cut(s)	869, 1190, 1526
MlyI	GAGTC	7 cut(s)	169, 305, 362, 376, 617, 659, 902
MmeI	TCCRAC	4 cut(s)	102, 1662, 1752, 1812
Mox20I	TGGCCA	3 cut(s)	869, 1190, 1526
MroXI	GAANNNNTTC	1 cut(s)	570
MscI	TGGCCA	3 cut(s)	869, 1190, 1526
MseI	TTAA	2 cut(s)	429, 1376
MslI	CAYNNNNRTG	1 cut(s)	1100
Msp20I	TGGCCA	3 cut(s)	869, 1190, 1526
MspA1I	CMGCKG	1 cut(s)	1021
MspI	CCGG	8 cut(s)	51, 812, 823, 857, 873, 1539, 1661, 1962
MspR9I	CCNGG	8 cut(s)	116, 812, 857, 1099, 1187, 1528, 1539, 1540
MunI	CAATTG	3 cut(s)	701, 1716, 2025
Mva1269I	GAATGC	1 cut(s)	20
MvaI	CCWGG	4 cut(s)	116, 1099, 1187, 1528
MvnI	CGCG	7 cut(s)	482, 484, 981, 1014, 1433, 1469, 1793
NciI	CCSGG	4 cut(s)	812, 857, 1539, 1540
NcoI	CCATGG	1 cut(s)	1560
NlaIII	CATG	7 cut(s)	64, 175, 547, 1006, 1360, 1416, 1564
NmeAIII	GCCGAG	2 cut(s)	1066, 1235
NmuCI	GTSAC	3 cut(s)	1306, 1672, 1807
NspV	TTCGAA	1 cut(s)	207
PauI	GCGCGC	1 cut(s)	480
PciSI	GCTCTTC	1 cut(s)	636
PcsI	WCGNNNNNNNCGW	5 cut(s)	444, 1038, 1386, 1778, 1910
PctI	GAATGC	1 cut(s)	20
PdmI	GAANNNNTTC	1 cut(s)	570
PfeI	GAWTC	6 cut(s)	134, 291, 914, 1199, 1237, 1942
Pfl23II	CGTACG	1 cut(s)	1249
PflFI	GACNNNGTC	3 cut(s)	443, 857, 1108
PinAI	ACCGGT	1 cut(s)	872
Ple19I	CGATCG	2 cut(s)	553, 889
PleI	GAGTC	7 cut(s)	169, 305, 361, 376, 617, 658, 901
PmaCI	CACGTG	2 cut(s)	930, 1499
PmlI	CACGTG	2 cut(s)	930, 1499
PpsI	GAGTC	7 cut(s)	169, 305, 361, 376, 617, 658, 901
Ppu21I	YACGTR	2 cut(s)	930, 1499
PpuMI	RGGWCCY	3 cut(s)	853, 1393, 1535
PshAI	GACNNNNGTC	1 cut(s)	1272
Psp5II	RGGWCCY	3 cut(s)	853, 1393, 1535
Psp6I	CCWGG	4 cut(s)	114, 1097, 1185, 1526
PspCI	CACGTG	2 cut(s)	930, 1499
PspGI	CCWGG	4 cut(s)	114, 1097, 1185, 1526
PspLI	CGTACG	1 cut(s)	1249
PspPI	GGNCC	6 cut(s)	853, 955, 1279, 1393, 1535, 1734
PspPPI	RGGWCCY	3 cut(s)	853, 1393, 1535
PstI	CTGCAG	1 cut(s)	288
PstNI	CAGNNNCTG	1 cut(s)	952
PsuI	RGATCY	1 cut(s)	1023
PsyI	GACNNNGTC	3 cut(s)	443, 857, 1108
PteI	GCGCGC	1 cut(s)	480
PvuI	CGATCG	2 cut(s)	553, 889
RsaI	GTAC	4 cut(s)	160, 275, 1251, 1472
RsaNI	GTAC	4 cut(s)	159, 274, 1250, 1471
RseI	CAYNNNNRTG	1 cut(s)	1100
SapI	GCTCTTC	1 cut(s)	636
SaqAI	TTAA	2 cut(s)	429, 1376
Sau96I	GGNCC	6 cut(s)	853, 955, 1279, 1393, 1535, 1734
SchI	GAGTC	7 cut(s)	169, 305, 362, 376, 617, 659, 902
ScrFI	CCNGG	8 cut(s)	116, 812, 857, 1099, 1187, 1528, 1539, 1540
SduI	GDGCHC	3 cut(s)	475, 1038, 1467
SfcI	CTRYAG	2 cut(s)	284, 1292
SfuI	TTCGAA	1 cut(s)	207
SinI	GGWCC	4 cut(s)	853, 1279, 1393, 1535
SmaI	CCCGGG	1 cut(s)	1540
SmiMI	CAYNNNNRTG	1 cut(s)	1100
Sse9I	AATT	7 cut(s)	615, 701, 1576, 1716, 1812, 1886, 2025
SspMI	CTAG	5 cut(s)	215, 620, 647, 1484, 2034
StyD4I	CCNGG	8 cut(s)	114, 810, 855, 1097, 1185, 1526, 1537, 1538
StyI	CCWWGG	2 cut(s)	510, 1560
TaaI	ACNGT	3 cut(s)	477, 1678, 1906
TaiI	ACGT	5 cut(s)	447, 932, 1255, 1272, 1501
TaqII	GACCGA	1 cut(s)	1262
TasI	AATT	7 cut(s)	615, 701, 1576, 1716, 1812, 1886, 2025
TatI	WGTACW	1 cut(s)	273
TfiI	GAWTC	6 cut(s)	134, 291, 914, 1199, 1237, 1942
Tru1I	TTAA	2 cut(s)	429, 1376
Tru9I	TTAA	2 cut(s)	429, 1376
TscAI	CASTG	2 cut(s)	482, 1369
TseFI	GTSAC	3 cut(s)	1306, 1672, 1807
TseI	GCWGC	5 cut(s)	141, 283, 324, 1018, 1154
Tsp45I	GTSAC	3 cut(s)	1306, 1672, 1807
TspDTI	ATGAA	2 cut(s)	49, 2001
TspGWI	ACGGA	2 cut(s)	458, 1383
TspMI	CCCGGG	1 cut(s)	1538
TspRI	CASTG	2 cut(s)	482, 1369
Tth111I	GACNNNGTC	3 cut(s)	443, 857, 1108
VneI	GTGCAC	2 cut(s)	471, 1463
VpaK11BI	GGWCC	4 cut(s)	853, 1279, 1393, 1535
XagI	CCTNNNNNAGG	2 cut(s)	113, 560
XapI	RAATTY	2 cut(s)	615, 1576
XbaI	TCTAGA	1 cut(s)	619
XcmI	CCANNNNNNNNNTGG	2 cut(s)	700, 1744
XmaI	CCCGGG	1 cut(s)	1538
XmnI	GAANNNNTTC	1 cut(s)	570
XspI	CTAG	5 cut(s)	215, 620, 647, 1484, 2034
ZraI	GACGTC	2 cut(s)	445, 1270

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.