MD13G1036500.v1.1

protein phosphatase 2C 15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0002720
Physical Location & Seq
Forward (+)
2538667 .. 2542408
3742 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1036500.v1.1.491

Sequence Viewer

Length: 1284 bp
ATGGCGTCCGGAGATAGGCGTCAGCATCATAATATGGTGCCTCTGGCTGCATTGATCAGCAGAGAGCTGAAGAGTGAGAAGATGGAGAAGCCAACTGTCAGATATGGGCATGCAGCTCAATCCAGGAAAGGGGAGGATTATTTCCTGATCAAGACTGATGGTCAGCGAGTACCCGGGAATTCTTCATCAGCATTCACCGCATTTGCGATCTTTGATGGGCACAATGGAAATGCTGCAGCAGTATATACAAGGGAGAATTTGTTAAAGCATGTGTTGGGTGCTATTCCCCGTGGGCTAGGAAGGGAGGAGTGGCTCCAAGCTTTACCTCGAGCCTTGGTTGCTGGGTTTGTGAAGACTGACAAGGATTTCCAGAGCAGAGGTGAAACTTCTGGAACAACAGCTACCTTTGTAATAGTTGATGGATGGACCGTGACAGTTGCATCAGTAGGAGACTCCCGTTGTATCTTAGATACTCAGGGTGGTGCCGTTTCCACCTTAACTGTTGATCACAGACTTGAAGAAAATGTAGAGGAGAGGGAGCGTGTTGCTGCAAGTGGTGGGGAAGTTGGGAGGTTGAGCATTGTTGGTGGTGCAGAGATTGGTCCCCTCCGTTGTTGGCCAGGGGGGTTATGCCTTTCTAGGTCAATTGGAGACATGGATGTTGGGGAGTTTATAGTTCCAATACCATATGTGAAACAAGTCAAGCTATCAAATGCCGGTGGGAGGCTTATAATTGCTTCGGATGGCATCTGGGATGCTATATCTTCCGAAATGGCAGCACAGTCTTGCCGTGGGTTGCCAGCTGAACTAGCTGCCAGACAAGTTGTGAAGGAGGCATTAAGGTCAAGGGGTCTAAAGGATGATACAACCTGCATAGTTGTTGACATAATCCCTCCTGATACTACGGTAGAGCCTTCACCTCCGCCAAAAAAGATTAACAAGTTTAGGACCCTGTTTTTCAGAAAGAAATCGTGTGCTTCTGCTAATAAACTATCCAAGAAGCTATCGGCTGTGGGTATTGTGGAGGAGTTATTTGAAGAAGGGTCTGCTATGCTTGCTGAAAGGTTGGGCAGCGATGAGTGCACACCACAATCAACATCTGGGCTGTTCATGTGTGCTGTATGCCAAGTTGACCTTGCACCGAGCGAGGGCATATCGGTTCACGCGGGTTCTATCTTCTCAACCAGTTCAAAGCCTTGGCAAGGGCCGTTCCTCTGTTCTGATTGTCGTAACAAGAAGGATGCCATGGAAGGAAAACGCCCTAGCGGAGTCAGAGTAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

428

Amino Acids

45.82

Weight (kDa)

8.24

Isoelectric Point (pI)

38.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 60 - 281 1.2e-34 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 731
Acc36I ACCTGC 1 cut(s) 878
AccB1I GGYRCC 2 cut(s) 37, 482
AccII CGCG 1 cut(s) 1166
AccIII TCCGGA 1 cut(s) 8
AciI CCGC 4 cut(s) 198, 923, 1166, 1266
AcoI YGGCCR 1 cut(s) 617
AcsI RAATTY 2 cut(s) 178, 256
AcuI CTGAAG 1 cut(s) 89
AcyI GRCGYC 2 cut(s) 5, 19
AfaI GTAC 1 cut(s) 171
AfiI CCNNNNNNNGG 5 cut(s) 15, 129, 723, 1148, 1202
AgsI TTSAA 3 cut(s) 518, 1037, 1191
AhdI GACNNNNNGTC 1 cut(s) 159
AjnI CCWGG 2 cut(s) 122, 619
AluBI AGCT 8 cut(s) 67, 116, 320, 401, 706, 803, 812, 1003
AluI AGCT 8 cut(s) 67, 116, 320, 401, 706, 803, 812, 1003
Alw21I GWGCWC 1 cut(s) 1085
Alw26I GTCTC 2 cut(s) 444, 645
Alw44I GTGCAC 1 cut(s) 1081
Ama87I CYCGRG 2 cut(s) 173, 327
Aor13HI TCCGGA 1 cut(s) 8
AoxI GGCC 2 cut(s) 617, 1205
ApaLI GTGCAC 1 cut(s) 1081
ApeKI GCWGC 8 cut(s) 47, 113, 233, 236, 548, 776, 812, 1071
ApoI RAATTY 2 cut(s) 178, 256
AspS9I GGNCC 4 cut(s) 426, 602, 948, 1205
AsuC2I CCSGG 2 cut(s) 174, 175
AsuHPI GGTGA 3 cut(s) 187, 392, 909
AvaI CYCGRG 2 cut(s) 173, 327
AvaII GGWCC 3 cut(s) 426, 602, 948
BaeGI GKGCMC 2 cut(s) 222, 1085
BaeI ACNNNNGTAYC 2 cut(s) 891, 924
BalI TGGCCA 1 cut(s) 619
BanI GGYRCC 2 cut(s) 37, 482
BbsI GAAGAC 1 cut(s) 359
Bbv12I GWGCWC 1 cut(s) 1085
BbvI GCAGC 8 cut(s) 34, 125, 220, 248, 535, 788, 799, 1083
BccI CCATC 6 cut(s) 76, 152, 209, 413, 417, 737
BceAI ACGGC 3 cut(s) 470, 774, 1192
BciT130I CCWGG 2 cut(s) 124, 621
BclI TGATCA 3 cut(s) 54, 147, 505
BcnI CCSGG 2 cut(s) 174, 175
BcoDI GTCTC 2 cut(s) 444, 645
BfaI CTAG 4 cut(s) 296, 639, 809, 1263
BfmI CTRYAG 1 cut(s) 234
BfuAI ACCTGC 1 cut(s) 878
BisI GCNGC 8 cut(s) 48, 114, 234, 237, 549, 777, 813, 1072
BlsI GCNGC 8 cut(s) 49, 115, 235, 238, 550, 778, 814, 1073
Bme1390I CCNGG 4 cut(s) 124, 174, 175, 621
Bme18I GGWCC 3 cut(s) 426, 602, 948
BmeRI GACNNNNNGTC 1 cut(s) 159
BmeT110I CYCGRG 2 cut(s) 173, 327
BmgT120I GGNCC 4 cut(s) 426, 602, 948, 1205
BmiI GGNNCC 5 cut(s) 39, 314, 484, 604, 950
BmrFI CCNGG 4 cut(s) 124, 174, 175, 621
BmsI GCATC 5 cut(s) 34, 449, 745, 756, 1231
BpiI GAAGAC 1 cut(s) 359
BpuMI CCSGG 2 cut(s) 174, 175
BsaHI GRCGYC 2 cut(s) 5, 19
BsaJI CCNNGG 7 cut(s) 173, 289, 333, 620, 790, 1196, 1245
BsaWI WCCGGW 1 cut(s) 8
BsaXI ACNNNNNCTCC 3 cut(s) 245, 275, 1260
Bsc4I CCNNNNNNNGG 5 cut(s) 15, 129, 723, 1148, 1202
Bse118I RCCGGY 1 cut(s) 716
Bse1I ACTGG 1 cut(s) 1185
BseAI TCCGGA 1 cut(s) 8
BseBI CCWGG 2 cut(s) 124, 621
BseDI CCNNGG 7 cut(s) 173, 289, 333, 620, 790, 1196, 1245
BseGI GGATG 6 cut(s) 428, 664, 748, 760, 865, 1246
BseLI CCNNNNNNNGG 5 cut(s) 15, 129, 723, 1148, 1202
BseMII CTCAG 1 cut(s) 488
BseNI ACTGG 1 cut(s) 1185
BseRI GAGGAG 3 cut(s) 320, 545, 1040
BseSI GKGCMC 2 cut(s) 222, 1085
BseXI GCAGC 8 cut(s) 34, 125, 220, 248, 535, 788, 799, 1083
BseYI CCCAGC 1 cut(s) 341
BsgI GTGCAG 1 cut(s) 612
Bsh1236I CGCG 1 cut(s) 1166
BshFI GGCC 2 cut(s) 619, 1207
BshNI GGYRCC 2 cut(s) 37, 482
BsiHKAI GWGCWC 1 cut(s) 1085
BsiHKCI CYCGRG 2 cut(s) 173, 327
BsiSI CCGG 3 cut(s) 9, 174, 717
BslFI GGGAC 1 cut(s) 588
BslI CCNNNNNNNGG 5 cut(s) 15, 129, 723, 1148, 1202
BsmAI GTCTC 2 cut(s) 444, 645
BsmFI GGGAC 1 cut(s) 588
BsmI GAATGC 1 cut(s) 191
BsnI GGCC 2 cut(s) 619, 1207
BsoBI CYCGRG 2 cut(s) 173, 327
Bsp1286I GDGCHC 2 cut(s) 222, 1085
Bsp13I TCCGGA 1 cut(s) 8
Bsp143I GATC 4 cut(s) 54, 147, 207, 505
Bsp19I CCATGG 1 cut(s) 1245
BspACI CCGC 4 cut(s) 198, 923, 1166, 1266
BspANI GGCC 2 cut(s) 619, 1207
BspCNI CTCAG 1 cut(s) 487
BspEI TCCGGA 1 cut(s) 8
BspFNI CGCG 1 cut(s) 1166
BspLI GGNNCC 5 cut(s) 39, 314, 484, 604, 950
BspMAI CTGCAG 1 cut(s) 238
BspMI ACCTGC 1 cut(s) 878
BspT107I GGYRCC 2 cut(s) 37, 482
BsrFI RCCGGY 1 cut(s) 716
BsrI ACTGG 1 cut(s) 1185
BssAI RCCGGY 1 cut(s) 716
BssECI CCNNGG 7 cut(s) 173, 289, 333, 620, 790, 1196, 1245
BssMI GATC 4 cut(s) 54, 147, 207, 505
BssNI GRCGYC 2 cut(s) 5, 19
BssT1I CCWWGG 3 cut(s) 333, 1196, 1245
Bst2UI CCWGG 2 cut(s) 124, 621
Bst4CI ACNGT 6 cut(s) 97, 430, 436, 502, 783, 907
Bst6I CTCTTC 1 cut(s) 65
BstACI GRCGYC 2 cut(s) 5, 19
BstC8I GCNNGC 3 cut(s) 111, 801, 1056
BstDEI CTNAG 2 cut(s) 466, 474
BstDSI CCRYGG 3 cut(s) 289, 790, 1245
BstENI CCTNNNNNAGG 1 cut(s) 1200
BstF5I GGATG 6 cut(s) 428, 664, 748, 760, 865, 1246
BstFNI CGCG 1 cut(s) 1166
BstKTI GATC 4 cut(s) 57, 150, 210, 508
BstMAI GTCTC 2 cut(s) 444, 645
BstMBI GATC 4 cut(s) 54, 147, 207, 505
BstMWI GCNNNNNNNGC 5 cut(s) 197, 338, 809, 1055, 1080
BstNI CCWGG 2 cut(s) 124, 621
BstNSI RCATGY 2 cut(s) 113, 272
BstSCI CCNGG 4 cut(s) 122, 172, 173, 619
BstSFI CTRYAG 1 cut(s) 234
BstSLI GKGCMC 2 cut(s) 222, 1085
BstUI CGCG 1 cut(s) 1166
BstV1I GCAGC 8 cut(s) 34, 125, 220, 248, 535, 788, 799, 1083
BstV2I GAAGAC 1 cut(s) 359
BsuRI GGCC 2 cut(s) 619, 1207
BtgI CCRYGG 3 cut(s) 289, 790, 1245
BtgZI GCGATG 1 cut(s) 1089
BtsCI GGATG 6 cut(s) 428, 664, 748, 760, 865, 1246
BveI ACCTGC 1 cut(s) 878
Cac8I GCNNGC 3 cut(s) 111, 801, 1056
Cfr10I RCCGGY 1 cut(s) 716
Cfr13I GGNCC 4 cut(s) 426, 602, 948, 1205
Cfr9I CCCGGG 1 cut(s) 173
CseI GACGC 1 cut(s) 8
Csp6I GTAC 1 cut(s) 170
CviAII CATG 5 cut(s) 110, 269, 655, 1111, 1246
CviQI GTAC 1 cut(s) 170
DdeI CTNAG 2 cut(s) 466, 474
DpnI GATC 4 cut(s) 56, 149, 209, 507
DpnII GATC 4 cut(s) 54, 147, 207, 505
DriI GACNNNNNGTC 1 cut(s) 159
EaeI YGGCCR 1 cut(s) 617
Eam1104I CTCTTC 1 cut(s) 65
Eam1105I GACNNNNNGTC 1 cut(s) 159
EarI CTCTTC 1 cut(s) 65
EciI GGCGGA 1 cut(s) 912
Eco130I CCWWGG 3 cut(s) 333, 1196, 1245
Eco47I GGWCC 3 cut(s) 426, 602, 948
Eco57I CTGAAG 1 cut(s) 89
Eco88I CYCGRG 2 cut(s) 173, 327
EcoNI CCTNNNNNAGG 1 cut(s) 1200
EcoO109I RGGNCCY 1 cut(s) 948
EcoRI GAATTC 1 cut(s) 178
EcoRII CCWGG 2 cut(s) 122, 619
EcoT14I CCWWGG 3 cut(s) 333, 1196, 1245
ErhI CCWWGG 3 cut(s) 333, 1196, 1245
FaeI CATG 5 cut(s) 113, 272, 658, 1114, 1249
FalI AAGNNNNNCTT 2 cut(s) 1119, 1151
FaqI GGGAC 1 cut(s) 588
FatI CATG 5 cut(s) 109, 268, 654, 1110, 1245
FauI CCCGC 1 cut(s) 1159
FauNDI CATATG 1 cut(s) 688
FbaI TGATCA 3 cut(s) 54, 147, 505
Fnu4HI GCNGC 8 cut(s) 48, 114, 234, 237, 549, 777, 813, 1072
FokI GGATG 6 cut(s) 435, 671, 755, 767, 872, 1253
Fsp4HI GCNGC 8 cut(s) 48, 114, 234, 237, 549, 777, 813, 1072
FspBI CTAG 4 cut(s) 296, 639, 809, 1263
GluI GCNGC 8 cut(s) 48, 114, 234, 237, 549, 777, 813, 1072
GsaI CCCAGC 1 cut(s) 345
HaeIII GGCC 2 cut(s) 619, 1207
HapII CCGG 3 cut(s) 9, 174, 717
HgaI GACGC 1 cut(s) 8
Hin1I GRCGYC 2 cut(s) 5, 19
Hin1II CATG 5 cut(s) 113, 272, 658, 1114, 1249
HincII GTYRAC 2 cut(s) 883, 1132
HindII GTYRAC 2 cut(s) 883, 1132
HindIII AAGCTT 1 cut(s) 318
HinfI GANTC 2 cut(s) 452, 1269
HpaII CCGG 3 cut(s) 9, 174, 717
HphI GGTGA 3 cut(s) 187, 392, 909
Hpy166II GTNNAC 4 cut(s) 883, 1083, 1132, 1162
Hpy188I TCNGA 6 cut(s) 101, 742, 769, 962, 1222, 1274
Hpy188III TCNNGA 6 cut(s) 9, 145, 151, 370, 390, 896
Hpy8I GTNNAC 4 cut(s) 883, 1083, 1132, 1162
HpyAV CCTTC 6 cut(s) 294, 823, 924, 1034, 1231, 1244
HpyCH4III ACNGT 6 cut(s) 97, 430, 436, 502, 783, 907
HpyCH4V TGCA 9 cut(s) 50, 113, 236, 440, 551, 593, 873, 1083, 1139
HpyF10VI GCNNNNNNNGC 5 cut(s) 197, 338, 809, 1055, 1080
HpyF3I CTNAG 2 cut(s) 466, 474
Hsp92I GRCGYC 2 cut(s) 5, 19
Hsp92II CATG 5 cut(s) 113, 272, 658, 1114, 1249
Kpn2I TCCGGA 1 cut(s) 8
Ksp22I TGATCA 3 cut(s) 54, 147, 505
Kzo9I GATC 4 cut(s) 54, 147, 207, 505
LmnI GCTCC 2 cut(s) 318, 538
Lsp1109I GCAGC 8 cut(s) 34, 125, 220, 248, 535, 788, 799, 1083
LweI GCATC 5 cut(s) 34, 449, 745, 756, 1231
MaeI CTAG 4 cut(s) 296, 639, 809, 1263
MaeIII GTNAC 2 cut(s) 430, 1229
MalI GATC 4 cut(s) 56, 149, 209, 507
MboI GATC 4 cut(s) 54, 147, 207, 505
MboII GAAGA 8 cut(s) 82, 91, 174, 364, 530, 756, 1049, 1168
MfeI CAATTG 1 cut(s) 645
MhlI GDGCHC 2 cut(s) 222, 1085
MlsI TGGCCA 1 cut(s) 619
MluCI AATT 4 cut(s) 178, 256, 645, 732
MluNI TGGCCA 1 cut(s) 619
MlyI GAGTC 2 cut(s) 446, 1278
Mox20I TGGCCA 1 cut(s) 619
MroI TCCGGA 1 cut(s) 8
MscI TGGCCA 1 cut(s) 619
MseI TTAA 4 cut(s) 263, 497, 839, 936
Msp20I TGGCCA 1 cut(s) 619
MspA1I CMGCKG 1 cut(s) 803
MspI CCGG 3 cut(s) 9, 174, 717
MspR9I CCNGG 4 cut(s) 124, 174, 175, 621
MunI CAATTG 1 cut(s) 645
Mva1269I GAATGC 1 cut(s) 191
MvaI CCWGG 2 cut(s) 124, 621
MvnI CGCG 1 cut(s) 1166
MwoI GCNNNNNNNGC 5 cut(s) 197, 338, 809, 1055, 1080
NciI CCSGG 2 cut(s) 174, 175
NcoI CCATGG 1 cut(s) 1245
NdeI CATATG 1 cut(s) 688
NdeII GATC 4 cut(s) 54, 147, 207, 505
NlaIII CATG 5 cut(s) 113, 272, 658, 1114, 1249
NlaIV GGNNCC 5 cut(s) 39, 314, 484, 604, 950
NmuCI GTSAC 1 cut(s) 430
NspI RCATGY 2 cut(s) 113, 272
PaeI GCATGC 1 cut(s) 113
PaeR7I CTCGAG 1 cut(s) 327
PctI GAATGC 1 cut(s) 191
PfoI TCCNGGA 1 cut(s) 122
PkrI GCNGC 8 cut(s) 49, 115, 235, 238, 550, 778, 814, 1073
PleI GAGTC 2 cut(s) 446, 1277
PpsI GAGTC 2 cut(s) 446, 1277
PpuMI RGGWCCY 1 cut(s) 948
PsiI TTATAA 1 cut(s) 731
Psp5II RGGWCCY 1 cut(s) 948
Psp6I CCWGG 2 cut(s) 122, 619
PspFI CCCAGC 1 cut(s) 341
PspGI CCWGG 2 cut(s) 122, 619
PspN4I GGNNCC 5 cut(s) 39, 314, 484, 604, 950
PspPI GGNCC 4 cut(s) 426, 602, 948, 1205
PspPPI RGGWCCY 1 cut(s) 948
PspXI VCTCGAGB 1 cut(s) 327
PsrI GAACNNNNNNTAC 2 cut(s) 385, 417
PstI CTGCAG 1 cut(s) 238
PvuII CAGCTG 1 cut(s) 803
RsaI GTAC 1 cut(s) 171
RsaNI GTAC 1 cut(s) 170
SaqAI TTAA 4 cut(s) 263, 497, 839, 936
SatI GCNGC 8 cut(s) 48, 114, 234, 237, 549, 777, 813, 1072
Sau3AI GATC 4 cut(s) 54, 147, 207, 505
Sau96I GGNCC 4 cut(s) 426, 602, 948, 1205
SchI GAGTC 2 cut(s) 446, 1278
ScrFI CCNGG 4 cut(s) 124, 174, 175, 621
SduI GDGCHC 2 cut(s) 222, 1085
SfaNI GCATC 5 cut(s) 34, 449, 745, 756, 1231
SfcI CTRYAG 1 cut(s) 234
Sfr274I CTCGAG 1 cut(s) 327
SinI GGWCC 3 cut(s) 426, 602, 948
SlaI CTCGAG 1 cut(s) 327
SmaI CCCGGG 1 cut(s) 175
SmlI CTYRAG 1 cut(s) 327
SmoI CTYRAG 1 cut(s) 327
SphI GCATGC 1 cut(s) 113
Sse9I AATT 4 cut(s) 178, 256, 645, 732
SsiI CCGC 4 cut(s) 198, 923, 1166, 1266
SspMI CTAG 4 cut(s) 296, 639, 809, 1263
StyD4I CCNGG 4 cut(s) 122, 172, 173, 619
StyI CCWWGG 3 cut(s) 333, 1196, 1245
TaaI ACNGT 6 cut(s) 97, 430, 436, 502, 783, 907
TaqI TCGA 1 cut(s) 328
TasI AATT 4 cut(s) 178, 256, 645, 732
Tru1I TTAA 4 cut(s) 263, 497, 839, 936
Tru9I TTAA 4 cut(s) 263, 497, 839, 936
TseFI GTSAC 1 cut(s) 430
TseI GCWGC 8 cut(s) 47, 113, 233, 236, 548, 776, 812, 1071
Tsp45I GTSAC 1 cut(s) 430
TspDTI ATGAA 2 cut(s) 174, 1099
TspGWI ACGGA 1 cut(s) 599
TspMI CCCGGG 1 cut(s) 173
VneI GTGCAC 1 cut(s) 1081
VpaK11BI GGWCC 3 cut(s) 426, 602, 948
XagI CCTNNNNNAGG 1 cut(s) 1200
XapI RAATTY 2 cut(s) 178, 256
XceI RCATGY 2 cut(s) 113, 272
XhoI CTCGAG 1 cut(s) 327
XmaI CCCGGG 1 cut(s) 173
XspI CTAG 4 cut(s) 296, 639, 809, 1263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.