Rw4G029090

protein phosphatase 2C 15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0002720
Physical Location & Seq
Forward (+)
55791453 .. 55794039
2587 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G029090.1

Sequence Viewer

Length: 882 bp
ATGGCACAGACAGCTCCACCACCACCGTCGCGCCACCGCCTCTTGCAGACCCCGACCGCCTTCAAGCCACCAACCCCGGCAGAGATCCAGCAAATCGCAACGCACACCACCACAGCCCCAAGACACGGGAATCAGCTGGAGTTGCTTTTGAAAAGTTCGAATTTCTGTGAACCATCGAGTTACCAAAATTTTAGTGCTTTTGAGATTGGTCCTCTTCTTTGTTGGCCAGGAGGTTTATGCCTTTCTAGGTCGATTGGAGACATGGATGTTGGGGAGTTCATAGTTCCAATACCATATGTGAAACAAGTCAAGCTATCAAATAGAGGAGGGAGGCTCATAATTGCTTCTGATGGAATCTGGGATGCCCTATACTCAGAAGAGGCAGCAAAATCTTGCCGTGGGTTGCCCGCTGAACTTGCCGCTAGACAAGTAGTGAAGGAGGCTTTAAGGTCAAGGGTTGTATCTTACAACCTGAAGAAGTCACGTGATTCTTCTCATAAACTATCCAAGAAGCTATCAGCTGTGGGTATCGTGGAAGAACTATTTGAAGAAGGCTCTGCCATGCTTGCTGAAAGGCTGGGCAATGATGAGTGCACAGCACAATCAACAACATCTGGGCTGTTTATGTGTGCTGTATGCCAAGTTGACCTTGCACCGAGTGAGGGTATATCTGTTCATGCTGGTTCCATTTTCTCCATGAGTTCAAAGCCTTGGCAAGGGCCTTTCCTCTGTTCTGATTGTCGTAATAAGAAGGATGCCATGGAAGGAAAACGCCCTAGCGGAGAATTGAAGAAGATCAACCCCGCTCTATTTCTATATCTGTGTGTTTTTCGTGCTAGTCTAAATATGGATGTCGTGAAGGTCATCCTTATTAAGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

293

Amino Acids

31.94

Weight (kDa)

8.72

Isoelectric Point (pI)

43.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 79 - 152 7.5e-10 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 806
AccII CGCG 1 cut(s) 31
AciI CCGC 6 cut(s) 37, 57, 408, 420, 780, 804
AclWI GGATC 1 cut(s) 79
AcoI YGGCCR 1 cut(s) 224
AcsI RAATTY 2 cut(s) 160, 187
AcuI CTGAAG 1 cut(s) 494
AcvI CACGTG 1 cut(s) 485
AdeI CACNNNGTG 1 cut(s) 659
AfiI CCNNNNNNNGG 3 cut(s) 125, 662, 716
AgsI TTSAA 5 cut(s) 64, 151, 548, 705, 790
AjnI CCWGG 1 cut(s) 226
AluBI AGCT 5 cut(s) 14, 136, 313, 514, 521
AluI AGCT 5 cut(s) 14, 136, 313, 514, 521
Alw21I GWGCWC 1 cut(s) 596
Alw26I GTCTC 1 cut(s) 252
Alw44I GTGCAC 1 cut(s) 592
AlwI GGATC 1 cut(s) 79
AoxI GGCC 2 cut(s) 224, 719
ApaLI GTGCAC 1 cut(s) 592
ApeKI GCWGC 1 cut(s) 383
ApoI RAATTY 2 cut(s) 160, 187
AspLEI GCGC 1 cut(s) 33
AspS9I GGNCC 2 cut(s) 209, 719
AsuC2I CCSGG 1 cut(s) 77
AsuII TTCGAA 1 cut(s) 158
AvaII GGWCC 1 cut(s) 209
BaeGI GKGCMC 1 cut(s) 596
BalI TGGCCA 1 cut(s) 226
BbrPI CACGTG 1 cut(s) 485
Bbv12I GWGCWC 1 cut(s) 596
BbvI GCAGC 1 cut(s) 395
BccI CCATC 2 cut(s) 181, 344
BceAI ACGGC 1 cut(s) 381
BciT130I CCWGG 1 cut(s) 228
BcnI CCSGG 1 cut(s) 77
BcoDI GTCTC 1 cut(s) 252
BfaI CTAG 4 cut(s) 246, 423, 777, 837
BisI GCNGC 2 cut(s) 384, 420
BlsI GCNGC 2 cut(s) 385, 421
Bme1390I CCNGG 2 cut(s) 77, 228
Bme18I GGWCC 1 cut(s) 209
BmgT120I GGNCC 2 cut(s) 209, 719
BmiI GGNNCC 1 cut(s) 685
BmrFI CCNGG 2 cut(s) 77, 228
BmsI GCATC 2 cut(s) 352, 745
BplI GAGNNNNNCTC 2 cut(s) 318, 350
BpmI CTGGAG 1 cut(s) 158
Bpu14I TTCGAA 1 cut(s) 158
BpuMI CCSGG 1 cut(s) 77
BsaAI YACGTR 1 cut(s) 485
BsaJI CCNNGG 4 cut(s) 75, 397, 710, 759
Bsc4I CCNNNNNNNGG 3 cut(s) 125, 662, 716
Bse3DI GCAATG 1 cut(s) 589
BseBI CCWGG 1 cut(s) 228
BseDI CCNNGG 4 cut(s) 75, 397, 710, 759
BseGI GGATG 5 cut(s) 271, 367, 760, 856, 864
BseLI CCNNNNNNNGG 3 cut(s) 125, 662, 716
BseMI GCAATG 1 cut(s) 589
BseMII CTCAG 1 cut(s) 387
BseRI GAGGAG 1 cut(s) 339
BseSI GKGCMC 1 cut(s) 596
BseXI GCAGC 1 cut(s) 395
BseYI CCCAGC 1 cut(s) 577
Bsh1236I CGCG 1 cut(s) 31
Bsh1285I CGRYCG 1 cut(s) 57
BshFI GGCC 2 cut(s) 226, 721
BsiEI CGRYCG 1 cut(s) 57
BsiHKAI GWGCWC 1 cut(s) 596
BsiSI CCGG 1 cut(s) 77
BslI CCNNNNNNNGG 3 cut(s) 125, 662, 716
BsmAI GTCTC 1 cut(s) 252
BsnI GGCC 2 cut(s) 226, 721
Bsp119I TTCGAA 1 cut(s) 158
Bsp1286I GDGCHC 1 cut(s) 596
Bsp143I GATC 2 cut(s) 84, 795
Bsp19I CCATGG 1 cut(s) 759
BspACI CCGC 6 cut(s) 37, 57, 408, 420, 780, 804
BspANI GGCC 2 cut(s) 226, 721
BspCNI CTCAG 1 cut(s) 386
BspFNI CGCG 1 cut(s) 31
BspLI GGNNCC 1 cut(s) 685
BspPI GGATC 1 cut(s) 79
BspT104I TTCGAA 1 cut(s) 158
BsrBI CCGCTC 1 cut(s) 806
BsrDI GCAATG 1 cut(s) 589
BssECI CCNNGG 4 cut(s) 75, 397, 710, 759
BssMI GATC 2 cut(s) 84, 795
BssT1I CCWWGG 2 cut(s) 710, 759
Bst2UI CCWGG 1 cut(s) 228
Bst4CI ACNGT 1 cut(s) 27
Bst6I CTCTTC 2 cut(s) 219, 372
BstBAI YACGTR 1 cut(s) 485
BstBI TTCGAA 1 cut(s) 158
BstC8I GCNNGC 2 cut(s) 408, 567
BstDEI CTNAG 1 cut(s) 373
BstDSI CCRYGG 2 cut(s) 397, 759
BstENI CCTNNNNNAGG 1 cut(s) 714
BstF5I GGATG 5 cut(s) 271, 367, 760, 856, 864
BstFNI CGCG 1 cut(s) 31
BstHHI GCGC 1 cut(s) 33
BstKTI GATC 2 cut(s) 87, 798
BstMAI GTCTC 1 cut(s) 252
BstMBI GATC 2 cut(s) 84, 795
BstMCI CGRYCG 1 cut(s) 57
BstMWI GCNNNNNNNGC 4 cut(s) 11, 142, 416, 566
BstNI CCWGG 1 cut(s) 228
BstSCI CCNGG 2 cut(s) 75, 226
BstSLI GKGCMC 1 cut(s) 596
BstUI CGCG 1 cut(s) 31
BstV1I GCAGC 1 cut(s) 395
BstX2I RGATCY 1 cut(s) 84
BstYI RGATCY 1 cut(s) 84
BsuRI GGCC 2 cut(s) 226, 721
BtgI CCRYGG 2 cut(s) 397, 759
BtsCI GGATG 5 cut(s) 271, 367, 760, 856, 864
Cac8I GCNNGC 2 cut(s) 408, 567
CfoI GCGC 1 cut(s) 33
Cfr13I GGNCC 2 cut(s) 209, 719
CviAII CATG 5 cut(s) 262, 562, 677, 697, 760
DdeI CTNAG 1 cut(s) 373
DpnI GATC 2 cut(s) 86, 797
DpnII GATC 2 cut(s) 84, 795
DraIII CACNNNGTG 1 cut(s) 659
EaeI YGGCCR 1 cut(s) 224
Eam1104I CTCTTC 2 cut(s) 219, 372
EarI CTCTTC 2 cut(s) 219, 372
Eco130I CCWWGG 2 cut(s) 710, 759
Eco47I GGWCC 1 cut(s) 209
Eco57I CTGAAG 1 cut(s) 494
Eco72I CACGTG 1 cut(s) 485
EcoNI CCTNNNNNAGG 1 cut(s) 714
EcoO109I RGGNCCY 1 cut(s) 719
EcoRII CCWGG 1 cut(s) 226
EcoT14I CCWWGG 2 cut(s) 710, 759
ErhI CCWWGG 2 cut(s) 710, 759
FaeI CATG 5 cut(s) 265, 565, 680, 700, 763
FalI AAGNNNNNCTT 2 cut(s) 633, 665
FatI CATG 5 cut(s) 261, 561, 676, 696, 759
FauI CCCGC 2 cut(s) 415, 811
FauNDI CATATG 1 cut(s) 295
Fnu4HI GCNGC 2 cut(s) 384, 420
FokI GGATG 5 cut(s) 278, 374, 767, 851, 863
Fsp4HI GCNGC 2 cut(s) 384, 420
FspBI CTAG 4 cut(s) 246, 423, 777, 837
GlaI GCGC 1 cut(s) 32
GluI GCNGC 2 cut(s) 384, 420
GsaI CCCAGC 1 cut(s) 581
GsuI CTGGAG 1 cut(s) 158
HaeIII GGCC 2 cut(s) 226, 721
HapII CCGG 1 cut(s) 77
HhaI GCGC 1 cut(s) 33
Hin1II CATG 5 cut(s) 265, 565, 680, 700, 763
Hin6I GCGC 1 cut(s) 31
HinP1I GCGC 1 cut(s) 31
HincII GTYRAC 1 cut(s) 646
HindII GTYRAC 1 cut(s) 646
HinfI GANTC 3 cut(s) 130, 354, 488
HpaII CCGG 1 cut(s) 77
Hpy166II GTNNAC 3 cut(s) 170, 594, 646
Hpy188I TCNGA 3 cut(s) 349, 376, 736
Hpy188III TCNNGA 1 cut(s) 856
Hpy8I GTNNAC 3 cut(s) 170, 594, 646
Hpy99I CGWCG 1 cut(s) 31
HpyAV CCTTC 6 cut(s) 70, 430, 545, 745, 758, 853
HpyCH4III ACNGT 1 cut(s) 27
HpyCH4IV ACGT 1 cut(s) 484
HpyCH4V TGCA 3 cut(s) 46, 594, 653
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 142, 416, 566
HpyF3I CTNAG 1 cut(s) 373
HpySE526I ACGT 1 cut(s) 484
Hsp92II CATG 5 cut(s) 265, 565, 680, 700, 763
HspAI GCGC 1 cut(s) 31
Kzo9I GATC 2 cut(s) 84, 795
LmnI GCTCC 1 cut(s) 19
Lsp1109I GCAGC 1 cut(s) 395
LweI GCATC 2 cut(s) 352, 745
MaeI CTAG 4 cut(s) 246, 423, 777, 837
MaeII ACGT 1 cut(s) 484
MaeIII GTNAC 2 cut(s) 179, 480
MalI GATC 2 cut(s) 86, 797
MbiI CCGCTC 1 cut(s) 806
MboI GATC 2 cut(s) 84, 795
MboII GAAGA 8 cut(s) 206, 389, 483, 487, 548, 560, 802, 805
MflI RGATCY 1 cut(s) 84
MhlI GDGCHC 1 cut(s) 596
MlsI TGGCCA 1 cut(s) 226
MluCI AATT 4 cut(s) 160, 187, 339, 785
MluNI TGGCCA 1 cut(s) 226
Mox20I TGGCCA 1 cut(s) 226
MscI TGGCCA 1 cut(s) 226
MseI TTAA 2 cut(s) 446, 873
Msp20I TGGCCA 1 cut(s) 226
MspA1I CMGCKG 3 cut(s) 136, 410, 521
MspI CCGG 1 cut(s) 77
MspR9I CCNGG 2 cut(s) 77, 228
MvaI CCWGG 1 cut(s) 228
MvnI CGCG 1 cut(s) 31
MwoI GCNNNNNNNGC 4 cut(s) 11, 142, 416, 566
NciI CCSGG 1 cut(s) 77
NcoI CCATGG 1 cut(s) 759
NdeI CATATG 1 cut(s) 295
NdeII GATC 2 cut(s) 84, 795
NlaIII CATG 5 cut(s) 265, 565, 680, 700, 763
NlaIV GGNNCC 1 cut(s) 685
NmuCI GTSAC 1 cut(s) 480
NspV TTCGAA 1 cut(s) 158
PfeI GAWTC 3 cut(s) 130, 354, 488
PkrI GCNGC 2 cut(s) 385, 421
PmaCI CACGTG 1 cut(s) 485
PmlI CACGTG 1 cut(s) 485
Ppu21I YACGTR 1 cut(s) 485
Psp6I CCWGG 1 cut(s) 226
PspCI CACGTG 1 cut(s) 485
PspFI CCCAGC 1 cut(s) 577
PspGI CCWGG 1 cut(s) 226
PspN4I GGNNCC 1 cut(s) 685
PspPI GGNCC 2 cut(s) 209, 719
PsuI RGATCY 1 cut(s) 84
PvuII CAGCTG 2 cut(s) 136, 521
SaqAI TTAA 2 cut(s) 446, 873
SatI GCNGC 2 cut(s) 384, 420
Sau3AI GATC 2 cut(s) 84, 795
Sau96I GGNCC 2 cut(s) 209, 719
ScrFI CCNGG 2 cut(s) 77, 228
SduI GDGCHC 1 cut(s) 596
SfaNI GCATC 2 cut(s) 352, 745
SfuI TTCGAA 1 cut(s) 158
SinI GGWCC 1 cut(s) 209
Sse9I AATT 4 cut(s) 160, 187, 339, 785
SsiI CCGC 6 cut(s) 37, 57, 408, 420, 780, 804
SspMI CTAG 4 cut(s) 246, 423, 777, 837
StyD4I CCNGG 2 cut(s) 75, 226
StyI CCWWGG 2 cut(s) 710, 759
TaaI ACNGT 1 cut(s) 27
TaiI ACGT 1 cut(s) 487
TaqI TCGA 3 cut(s) 158, 176, 251
TasI AATT 4 cut(s) 160, 187, 339, 785
TauI GCSGC 1 cut(s) 422
TfiI GAWTC 3 cut(s) 130, 354, 488
Tru1I TTAA 2 cut(s) 446, 873
Tru9I TTAA 2 cut(s) 446, 873
TseFI GTSAC 1 cut(s) 480
TseI GCWGC 1 cut(s) 383
Tsp45I GTSAC 1 cut(s) 480
TspDTI ATGAA 2 cut(s) 268, 665
VneI GTGCAC 1 cut(s) 592
VpaK11BI GGWCC 1 cut(s) 209
XagI CCTNNNNNAGG 1 cut(s) 714
XapI RAATTY 2 cut(s) 160, 187
XspI CTAG 4 cut(s) 246, 423, 777, 837
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.