RLG00000006658

protein phosphatase 2C 15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0002720
Physical Location & Seq
Reverse (-)
9418965 .. 9421015
2051 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006658

Sequence Viewer

Length: 753 bp
ATGGAAAAGCCGACTGTCAGATACGGTCATGCGGCTCAATCCAGGAAAGGGGAGGATTATTTTCTGATCAAGACTGATGGGTACAATGGAAATGCAGCAGCCATATATACAAGAGAGAACTTGTTAAATCATGTGTTGGGTGCTATTCCAAGGGGGCTAGGAAGGGAGGAGTGGCTTCAAGCCTTACCTCGGGCCTTGGTTGCAGGGTTTGTGAAGGCTCATAAGGATTTCCAAAGTAGAGGAGAAGCTTCTGGAACAACAGCTACTGTTGTAATAGTTGATGGGTGGACTGTTACAGTAGCATCAGTAGGAGACTCCCGTTGTATCTTAGATACTCAGGGTGGTGCGGTTTCCACTTTAAATGTTGATCATAGACTTGAAGAGAGTGTAGAAGAGAGGGGAAGTCGGGAGGTTGAGCATTGTTGGTGGTGCGAAGATTGGTCCTCTTCGTTGTTGGCCAGGAGGTTTATGCTTTTCTCAGTCGATTGGAGACATGGATGTTGTGGAGTTCATAGTTCCAATACATATGTGAAACAGGTCAAGGAGGCTTTAAGGTCAAGGGGTTTAAAGGATGATACAACCTGTATAGTTGTTGACATTATCCCTCCTGATAACTCGGTACAGCCTACAACTCCCCCAAAAAAGCAGAACAAGCTCAGGGCCCTGTTGTTCAGGAAGAAGTCACGTGATTCTGCTCATAAACTATCCAAGAAGCTAGCTATCAGCTGTGGGTATCGTGGAAGAACTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

251

Amino Acids

27.86

Weight (kDa)

9.27

Isoelectric Point (pI)

28.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 12 - 132 6.7e-12 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 32, 347
AcoI YGGCCR 1 cut(s) 456
AcvI CACGTG 1 cut(s) 686
AfaI GTAC 2 cut(s) 83, 621
AfiI CCNNNNNNNGG 2 cut(s) 48, 189
AgsI TTSAA 2 cut(s) 179, 380
AjnI CCWGG 2 cut(s) 41, 458
AluBI AGCT 6 cut(s) 248, 263, 655, 715, 719, 726
AluI AGCT 6 cut(s) 248, 263, 655, 715, 719, 726
Alw26I GTCTC 2 cut(s) 306, 484
AlwNI CAGNNNCTG 1 cut(s) 266
Ama87I CYCGRG 1 cut(s) 189
AoxI GGCC 3 cut(s) 192, 456, 660
ApaI GGGCCC 1 cut(s) 664
ApeKI GCWGC 2 cut(s) 95, 98
AspS9I GGNCC 4 cut(s) 192, 441, 660, 661
AsuNHI GCTAGC 1 cut(s) 715
AvaI CYCGRG 1 cut(s) 189
AvaII GGWCC 1 cut(s) 441
BaeGI GKGCMC 1 cut(s) 664
BalI TGGCCA 1 cut(s) 458
BanII GRGCYC 1 cut(s) 664
BbrPI CACGTG 1 cut(s) 686
BbvI GCAGC 2 cut(s) 107, 110
BccI CCATC 2 cut(s) 71, 275
BciT130I CCWGG 2 cut(s) 43, 460
BclI TGATCA 2 cut(s) 66, 367
BcoDI GTCTC 2 cut(s) 306, 484
BfaI CTAG 2 cut(s) 158, 716
BisI GCNGC 3 cut(s) 33, 96, 99
BlsI GCNGC 3 cut(s) 34, 97, 100
Bme1390I CCNGG 2 cut(s) 43, 460
Bme18I GGWCC 1 cut(s) 441
BmeT110I CYCGRG 1 cut(s) 189
BmgT120I GGNCC 4 cut(s) 192, 441, 660, 661
BmiI GGNNCC 1 cut(s) 662
BmrFI CCNGG 2 cut(s) 43, 460
BmsI GCATC 1 cut(s) 311
BmtI GCTAGC 1 cut(s) 719
Bpu10I CCTNAGC 1 cut(s) 656
BsaAI YACGTR 1 cut(s) 686
BsaJI CCNNGG 3 cut(s) 149, 188, 195
Bsc4I CCNNNNNNNGG 2 cut(s) 48, 189
BseBI CCWGG 2 cut(s) 43, 460
BseDI CCNNGG 3 cut(s) 149, 188, 195
BseGI GGATG 2 cut(s) 503, 577
BseLI CCNNNNNNNGG 2 cut(s) 48, 189
BseMII CTCAG 3 cut(s) 350, 492, 670
BseRI GAGGAG 2 cut(s) 182, 255
BseSI GKGCMC 1 cut(s) 664
BseXI GCAGC 2 cut(s) 107, 110
BshFI GGCC 3 cut(s) 194, 458, 662
BsiHKCI CYCGRG 1 cut(s) 189
BslI CCNNNNNNNGG 2 cut(s) 48, 189
BsmAI GTCTC 2 cut(s) 306, 484
BsnI GGCC 3 cut(s) 194, 458, 662
BsoBI CYCGRG 1 cut(s) 189
Bsp120I GGGCCC 1 cut(s) 660
Bsp1286I GDGCHC 1 cut(s) 664
Bsp143I GATC 2 cut(s) 66, 367
BspACI CCGC 2 cut(s) 32, 347
BspANI GGCC 3 cut(s) 194, 458, 662
BspCNI CTCAG 3 cut(s) 349, 491, 669
BspLI GGNNCC 1 cut(s) 662
BspOI GCTAGC 1 cut(s) 719
BssECI CCNNGG 3 cut(s) 149, 188, 195
BssMI GATC 2 cut(s) 66, 367
BssT1I CCWWGG 2 cut(s) 149, 195
Bst2UI CCWGG 2 cut(s) 43, 460
Bst4CI ACNGT 5 cut(s) 16, 26, 268, 292, 298
Bst6I CTCTTC 3 cut(s) 375, 387, 451
BstBAI YACGTR 1 cut(s) 686
BstC8I GCNNGC 1 cut(s) 717
BstDEI CTNAG 4 cut(s) 328, 336, 478, 656
BstF5I GGATG 2 cut(s) 503, 577
BstKTI GATC 2 cut(s) 69, 370
BstMAI GTCTC 2 cut(s) 306, 484
BstMBI GATC 2 cut(s) 66, 367
BstMWI GCNNNNNNNGC 2 cut(s) 200, 652
BstNI CCWGG 2 cut(s) 43, 460
BstSCI CCNGG 2 cut(s) 41, 458
BstSLI GKGCMC 1 cut(s) 664
BstV1I GCAGC 2 cut(s) 107, 110
BsuRI GGCC 3 cut(s) 194, 458, 662
BtsCI GGATG 2 cut(s) 503, 577
Cac8I GCNNGC 1 cut(s) 717
CaiI CAGNNNCTG 1 cut(s) 266
Cfr13I GGNCC 4 cut(s) 192, 441, 660, 661
Csp6I GTAC 2 cut(s) 82, 620
CviAII CATG 3 cut(s) 29, 131, 494
CviQI GTAC 2 cut(s) 82, 620
DdeI CTNAG 4 cut(s) 328, 336, 478, 656
DpnI GATC 2 cut(s) 68, 369
DpnII GATC 2 cut(s) 66, 367
DraI TTTAAA 2 cut(s) 360, 567
EaeI YGGCCR 1 cut(s) 456
Eam1104I CTCTTC 3 cut(s) 375, 387, 451
EarI CTCTTC 3 cut(s) 375, 387, 451
Eco130I CCWWGG 2 cut(s) 149, 195
Eco24I GRGCYC 1 cut(s) 664
Eco47I GGWCC 1 cut(s) 441
Eco72I CACGTG 1 cut(s) 686
Eco88I CYCGRG 1 cut(s) 189
EcoO109I RGGNCCY 2 cut(s) 660, 661
EcoRII CCWGG 2 cut(s) 41, 458
EcoT14I CCWWGG 2 cut(s) 149, 195
EcoT38I GRGCYC 1 cut(s) 664
ErhI CCWWGG 2 cut(s) 149, 195
FaeI CATG 3 cut(s) 32, 134, 497
FatI CATG 3 cut(s) 28, 130, 493
FauNDI CATATG 1 cut(s) 526
FbaI TGATCA 2 cut(s) 66, 367
Fnu4HI GCNGC 3 cut(s) 33, 96, 99
FokI GGATG 2 cut(s) 510, 584
FriOI GRGCYC 1 cut(s) 664
Fsp4HI GCNGC 3 cut(s) 33, 96, 99
FspBI CTAG 2 cut(s) 158, 716
GluI GCNGC 3 cut(s) 33, 96, 99
HaeIII GGCC 3 cut(s) 194, 458, 662
Hin1II CATG 3 cut(s) 32, 134, 497
HincII GTYRAC 1 cut(s) 595
HindII GTYRAC 1 cut(s) 595
HindIII AAGCTT 1 cut(s) 246
HinfI GANTC 2 cut(s) 314, 689
Hpy166II GTNNAC 2 cut(s) 288, 595
Hpy188I TCNGA 2 cut(s) 20, 66
Hpy188III TCNNGA 5 cut(s) 70, 252, 407, 608, 673
Hpy8I GTNNAC 2 cut(s) 288, 595
HpyAV CCTTC 2 cut(s) 156, 208
HpyCH4III ACNGT 5 cut(s) 16, 26, 268, 292, 298
HpyCH4IV ACGT 1 cut(s) 685
HpyCH4V TGCA 2 cut(s) 95, 203
HpyF10VI GCNNNNNNNGC 2 cut(s) 200, 652
HpyF3I CTNAG 4 cut(s) 328, 336, 478, 656
HpySE526I ACGT 1 cut(s) 685
Hsp92II CATG 3 cut(s) 32, 134, 497
Ksp22I TGATCA 2 cut(s) 66, 367
Kzo9I GATC 2 cut(s) 66, 367
Lsp1109I GCAGC 2 cut(s) 107, 110
LweI GCATC 1 cut(s) 311
MaeI CTAG 2 cut(s) 158, 716
MaeII ACGT 1 cut(s) 685
MaeIII GTNAC 2 cut(s) 292, 681
MalI GATC 2 cut(s) 68, 369
MboI GATC 2 cut(s) 66, 367
MboII GAAGA 6 cut(s) 392, 404, 438, 446, 688, 753
MhlI GDGCHC 1 cut(s) 664
MlsI TGGCCA 1 cut(s) 458
MluNI TGGCCA 1 cut(s) 458
MlyI GAGTC 1 cut(s) 308
Mox20I TGGCCA 1 cut(s) 458
MscI TGGCCA 1 cut(s) 458
MseI TTAA 4 cut(s) 125, 359, 551, 566
Msp20I TGGCCA 1 cut(s) 458
MspA1I CMGCKG 1 cut(s) 726
MspR9I CCNGG 2 cut(s) 43, 460
MvaI CCWGG 2 cut(s) 43, 460
MwoI GCNNNNNNNGC 2 cut(s) 200, 652
NdeI CATATG 1 cut(s) 526
NdeII GATC 2 cut(s) 66, 367
NheI GCTAGC 1 cut(s) 715
NlaIII CATG 3 cut(s) 32, 134, 497
NlaIV GGNNCC 1 cut(s) 662
NmuCI GTSAC 1 cut(s) 681
PfeI GAWTC 1 cut(s) 689
PfoI TCCNGGA 1 cut(s) 41
PkrI GCNGC 3 cut(s) 34, 97, 100
PleI GAGTC 1 cut(s) 308
PmaCI CACGTG 1 cut(s) 686
PmlI CACGTG 1 cut(s) 686
PpsI GAGTC 1 cut(s) 308
Ppu21I YACGTR 1 cut(s) 686
Psp6I CCWGG 2 cut(s) 41, 458
PspCI CACGTG 1 cut(s) 686
PspGI CCWGG 2 cut(s) 41, 458
PspN4I GGNNCC 1 cut(s) 662
PspOMI GGGCCC 1 cut(s) 660
PspPI GGNCC 4 cut(s) 192, 441, 660, 661
PsrI GAACNNNNNNTAC 2 cut(s) 247, 279
PstNI CAGNNNCTG 1 cut(s) 266
PvuII CAGCTG 1 cut(s) 726
RsaI GTAC 2 cut(s) 83, 621
RsaNI GTAC 2 cut(s) 82, 620
SaqAI TTAA 4 cut(s) 125, 359, 551, 566
SatI GCNGC 3 cut(s) 33, 96, 99
Sau3AI GATC 2 cut(s) 66, 367
Sau96I GGNCC 4 cut(s) 192, 441, 660, 661
SchI GAGTC 1 cut(s) 308
ScrFI CCNGG 2 cut(s) 43, 460
SduI GDGCHC 1 cut(s) 664
SfaNI GCATC 1 cut(s) 311
SinI GGWCC 1 cut(s) 441
SsiI CCGC 2 cut(s) 32, 347
SspMI CTAG 2 cut(s) 158, 716
StyD4I CCNGG 2 cut(s) 41, 458
StyI CCWWGG 2 cut(s) 149, 195
TaaI ACNGT 5 cut(s) 16, 26, 268, 292, 298
TaiI ACGT 1 cut(s) 688
TaqI TCGA 1 cut(s) 483
TauI GCSGC 1 cut(s) 35
TfiI GAWTC 1 cut(s) 689
Tru1I TTAA 4 cut(s) 125, 359, 551, 566
Tru9I TTAA 4 cut(s) 125, 359, 551, 566
TseFI GTSAC 1 cut(s) 681
TseI GCWGC 2 cut(s) 95, 98
Tsp45I GTSAC 1 cut(s) 681
TspDTI ATGAA 1 cut(s) 500
VpaK11BI GGWCC 1 cut(s) 441
XspI CTAG 2 cut(s) 158, 716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.