Rroxscaffold_5G00375880

protein phosphatase 2C 15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0002720
Physical Location & Seq
Forward (+)
56640699 .. 56644799
4101 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00375880.1

Sequence Viewer

Length: 1413 bp
ATGGGGTCTACAGAAGGGAGGCGTAATCATCATAATCTGGTGCCTCTTGCTGCATTGATCAGCAGAGAGATGAAGAATGAGAAGATGGAAAAACCGACAGTCAGATACGGGCATGCGGCTCAATCCAGGAAAGGGGAGGATTATTTTCTGATCAAGACTGATGGTCAGCGAGTGCCTGGAAATTCTTCATCAACATTTTCTGCATTTGCGATCTTTGACGGGCACAATGGAAACGCAGCAGCCATATATACAAGGGAGAACTTGTTAAATCATGTGATGGGTGCTATTCCCAGGGGGCTAGGAAGGGAGGAGTGGCTCCAAGCCTTACCTCGGGCCTTGGTTGCAGGGTTTGTGAAGGCCGATAAGGATTTCCAAAGTAGAGGCATGAGGGAGCGTGTTGCTGCAAGTGGCGGCGAAGTAGGGAGGTTGAGCATTGTTGGTGGTGCAGAGATTGGTCCTCTTCGATGTTGGCCAGGAGGTTTATGCCTTTCTAGGTCGATTGGAGACATGGATGTTGGGGAGTTCATAGTTCCAATACCATATGTGAAACAAGTCAAGCTATCAAATAGAGGAGGGAGGCTCATAATTGCTTCTGATGGCATCTGGGATGCCCTATCCTCAGAAGAGGCAGCAAAATCTTGCCGTGGGTTGCCCGCTGAACTTGCTGCTAGACAAGTGGTGAAGGAGGCTTTAAGGTCAAGGGGTTTAAAGGATGATACAACCTGTATAGTTGTTGACATAATCCCTCCTGATAACTCGGTACAGCCTACAACTCCCCCAAAAAAGCAGAACAAGCTCAGGGCCCTGTTGTTCAGGAAGAAGTCACGTGATTCTGCACATAAACTATCCAAGAAGCTATCAGCTGTGGGTATCGTGGAAGAACTATTTGAAGAAGGCTCTGCCATGCTTGCTGAAAGGCTGGGCAATGATGATTGCACGGCACAATCAACAACATCTGGGCTGTTTATGTGTGCTGTATGCCAAGTTGACCTTGCACCAAGTGAGGGCATATCTGTTCATGCTGGTTCCATCTTCTCCATGAGTTCAAAACCTTGGCAAGGGCCTTTCCTCTGTTCTGATTGTCGTAATAAGAAGGATGCCATGGAAGGAAAACGCCCTAGCGGAAAGAGAAACCATACAGGTTGTCCAGTCTACTACCGTAATGACATGATAAGCAATATCTTCTTCCCCTTCAAAAATAAGATGCATTCTCCTGAATGCAGATTGTACTGTGTTGTTCTGAACGCTCTAGTCCGAAGATCCAACTCAACGAGGGAGGGTACTAGCAAGAGCTTGAAAGCATCAGAGTCAAATATAATTTCTATCATGGAGAAAGAGCTTCAGACGCGTTTAGAAGCAGATCCCTGCAATGATGAAGTTTTCGACGCTTCGAGTATTCCCAGCAGTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

470

Amino Acids

51.34

Weight (kDa)

9.07

Isoelectric Point (pI)

46.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 130 - 232 2.2e-12 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 40
AccI GTMKAC 2 cut(s) 8, 1152
AccII CGCG 1 cut(s) 1348
AciI CCGC 4 cut(s) 116, 411, 654, 1122
AclWI GGATC 2 cut(s) 1254, 1355
AcoI YGGCCR 1 cut(s) 470
AcsI RAATTY 1 cut(s) 181
AcuI CTGAAG 1 cut(s) 1325
AcvI CACGTG 1 cut(s) 827
AdeI CACNNNGTG 1 cut(s) 1001
AfaI GTAC 3 cut(s) 762, 1229, 1282
AfiI CCNNNNNNNGG 4 cut(s) 132, 330, 1004, 1058
AflIII ACRYGT 1 cut(s) 1346
AgsI TTSAA 4 cut(s) 890, 1047, 1195, 1297
AhdI GACNNNNNGTC 1 cut(s) 162
AjnI CCWGG 4 cut(s) 125, 175, 290, 472
AluBI AGCT 6 cut(s) 559, 796, 856, 863, 1293, 1339
AluI AGCT 6 cut(s) 559, 796, 856, 863, 1293, 1339
Alw26I GTCTC 1 cut(s) 498
AlwI GGATC 2 cut(s) 1254, 1355
Ama87I CYCGRG 1 cut(s) 330
AoxI GGCC 5 cut(s) 333, 357, 470, 801, 1061
ApaI GGGCCC 1 cut(s) 805
ApeKI GCWGC 6 cut(s) 50, 236, 239, 401, 629, 665
ApoI RAATTY 1 cut(s) 181
Asp700I GAANNNNTTC 1 cut(s) 184
AspS9I GGNCC 5 cut(s) 333, 455, 801, 802, 1061
AsuHPI GGTGA 1 cut(s) 691
AvaI CYCGRG 1 cut(s) 330
AvaII GGWCC 1 cut(s) 455
BaeGI GKGCMC 2 cut(s) 225, 805
BalI TGGCCA 1 cut(s) 472
BanI GGYRCC 1 cut(s) 40
BanII GRGCYC 1 cut(s) 805
BbrPI CACGTG 1 cut(s) 827
BbvI GCAGC 6 cut(s) 37, 248, 251, 388, 641, 652
BccI CCATC 5 cut(s) 79, 155, 271, 590, 1037
BceAI ACGGC 2 cut(s) 627, 954
BciT130I CCWGG 4 cut(s) 127, 177, 292, 474
BclI TGATCA 2 cut(s) 57, 150
BcoDI GTCTC 1 cut(s) 498
BfaI CTAG 6 cut(s) 299, 492, 669, 1119, 1250, 1284
BfmI CTRYAG 1 cut(s) 9
BisI GCNGC 8 cut(s) 51, 117, 237, 240, 402, 412, 630, 666
BlsI GCNGC 8 cut(s) 52, 118, 238, 241, 403, 413, 631, 667
Bme1390I CCNGG 4 cut(s) 127, 177, 292, 474
Bme18I GGWCC 1 cut(s) 455
BmeRI GACNNNNNGTC 1 cut(s) 162
BmeT110I CYCGRG 1 cut(s) 330
BmgT120I GGNCC 5 cut(s) 333, 455, 801, 802, 1061
BmiI GGNNCC 4 cut(s) 42, 317, 803, 1027
BmrFI CCNGG 4 cut(s) 127, 177, 292, 474
BmsI GCATC 5 cut(s) 598, 609, 1087, 1194, 1310
BplI GAGNNNNNCTC 2 cut(s) 564, 596
Bpu10I CCTNAGC 1 cut(s) 797
BsaAI YACGTR 1 cut(s) 827
BsaJI CCNNGG 7 cut(s) 290, 291, 329, 336, 643, 1052, 1101
BsaXI ACNNNNNCTCC 2 cut(s) 248, 278
Bsc4I CCNNNNNNNGG 4 cut(s) 132, 330, 1004, 1058
Bse1I ACTGG 1 cut(s) 1148
Bse3DI GCAATG 2 cut(s) 931, 1375
BseBI CCWGG 4 cut(s) 127, 177, 292, 474
BseDI CCNNGG 7 cut(s) 290, 291, 329, 336, 643, 1052, 1101
BseGI GGATG 4 cut(s) 517, 613, 718, 1102
BseLI CCNNNNNNNGG 4 cut(s) 132, 330, 1004, 1058
BseMI GCAATG 2 cut(s) 931, 1375
BseMII CTCAG 2 cut(s) 633, 811
BseNI ACTGG 1 cut(s) 1148
BseRI GAGGAG 2 cut(s) 323, 585
BseSI GKGCMC 2 cut(s) 225, 805
BseXI GCAGC 6 cut(s) 37, 248, 251, 388, 641, 652
BseYI CCCAGC 2 cut(s) 919, 1400
BsgI GTGCAG 2 cut(s) 465, 819
Bsh1236I CGCG 1 cut(s) 1348
BshFI GGCC 5 cut(s) 335, 359, 472, 803, 1063
BshNI GGYRCC 1 cut(s) 40
BsiHKCI CYCGRG 1 cut(s) 330
BslI CCNNNNNNNGG 4 cut(s) 132, 330, 1004, 1058
BsmAI GTCTC 1 cut(s) 498
BsmI GAATGC 2 cut(s) 1207, 1223
BsnI GGCC 5 cut(s) 335, 359, 472, 803, 1063
BsoBI CYCGRG 1 cut(s) 330
Bsp120I GGGCCC 1 cut(s) 801
Bsp1286I GDGCHC 2 cut(s) 225, 805
Bsp143I GATC 5 cut(s) 57, 150, 210, 1259, 1360
Bsp19I CCATGG 1 cut(s) 1101
BspACI CCGC 4 cut(s) 116, 411, 654, 1122
BspANI GGCC 5 cut(s) 335, 359, 472, 803, 1063
BspCNI CTCAG 2 cut(s) 632, 810
BspFNI CGCG 1 cut(s) 1348
BspLI GGNNCC 4 cut(s) 42, 317, 803, 1027
BspPI GGATC 2 cut(s) 1254, 1355
BspT107I GGYRCC 1 cut(s) 40
BsrDI GCAATG 2 cut(s) 931, 1375
BsrI ACTGG 1 cut(s) 1148
BssECI CCNNGG 7 cut(s) 290, 291, 329, 336, 643, 1052, 1101
BssMI GATC 5 cut(s) 57, 150, 210, 1259, 1360
BssT1I CCWWGG 3 cut(s) 336, 1052, 1101
Bst2UI CCWGG 4 cut(s) 127, 177, 292, 474
Bst4CI ACNGT 3 cut(s) 100, 1160, 1232
Bst6I CTCTTC 2 cut(s) 465, 618
BstBAI YACGTR 1 cut(s) 827
BstC8I GCNNGC 3 cut(s) 114, 654, 909
BstDEI CTNAG 2 cut(s) 619, 797
BstDSI CCRYGG 2 cut(s) 643, 1101
BstENI CCTNNNNNAGG 1 cut(s) 1056
BstF5I GGATG 4 cut(s) 517, 613, 718, 1102
BstFNI CGCG 1 cut(s) 1348
BstKTI GATC 5 cut(s) 60, 153, 213, 1262, 1363
BstMAI GTCTC 1 cut(s) 498
BstMBI GATC 5 cut(s) 57, 150, 210, 1259, 1360
BstMWI GCNNNNNNNGC 5 cut(s) 341, 662, 793, 908, 1345
BstNI CCWGG 4 cut(s) 127, 177, 292, 474
BstNSI RCATGY 1 cut(s) 116
BstSCI CCNGG 4 cut(s) 125, 175, 290, 472
BstSFI CTRYAG 1 cut(s) 9
BstSLI GKGCMC 2 cut(s) 225, 805
BstUI CGCG 1 cut(s) 1348
BstV1I GCAGC 6 cut(s) 37, 248, 251, 388, 641, 652
BstX2I RGATCY 2 cut(s) 1259, 1360
BstYI RGATCY 2 cut(s) 1259, 1360
BsuRI GGCC 5 cut(s) 335, 359, 472, 803, 1063
BtgI CCRYGG 2 cut(s) 643, 1101
BtsCI GGATG 4 cut(s) 517, 613, 718, 1102
Cac8I GCNNGC 3 cut(s) 114, 654, 909
Cfr13I GGNCC 5 cut(s) 333, 455, 801, 802, 1061
CseI GACGC 2 cut(s) 1354, 1394
Csp6I GTAC 3 cut(s) 761, 1228, 1281
CviQI GTAC 3 cut(s) 761, 1228, 1281
DdeI CTNAG 2 cut(s) 619, 797
DpnI GATC 5 cut(s) 59, 152, 212, 1261, 1362
DpnII GATC 5 cut(s) 57, 150, 210, 1259, 1360
DraI TTTAAA 1 cut(s) 708
DraIII CACNNNGTG 1 cut(s) 1001
DriI GACNNNNNGTC 1 cut(s) 162
EaeI YGGCCR 1 cut(s) 470
Eam1104I CTCTTC 2 cut(s) 465, 618
Eam1105I GACNNNNNGTC 1 cut(s) 162
EarI CTCTTC 2 cut(s) 465, 618
Eco130I CCWWGG 3 cut(s) 336, 1052, 1101
Eco24I GRGCYC 1 cut(s) 805
Eco47I GGWCC 1 cut(s) 455
Eco57I CTGAAG 1 cut(s) 1325
Eco72I CACGTG 1 cut(s) 827
Eco88I CYCGRG 1 cut(s) 330
EcoNI CCTNNNNNAGG 1 cut(s) 1056
EcoO109I RGGNCCY 3 cut(s) 801, 802, 1061
EcoRII CCWGG 4 cut(s) 125, 175, 290, 472
EcoT14I CCWWGG 3 cut(s) 336, 1052, 1101
EcoT22I ATGCAT 1 cut(s) 1209
EcoT38I GRGCYC 1 cut(s) 805
ErhI CCWWGG 3 cut(s) 336, 1052, 1101
FalI AAGNNNNNCTT 2 cut(s) 975, 1007
FauI CCCGC 1 cut(s) 661
FauNDI CATATG 1 cut(s) 541
FbaI TGATCA 2 cut(s) 57, 150
FblI GTMKAC 2 cut(s) 8, 1152
Fnu4HI GCNGC 8 cut(s) 51, 117, 237, 240, 402, 412, 630, 666
FokI GGATG 4 cut(s) 524, 620, 725, 1109
FriOI GRGCYC 1 cut(s) 805
Fsp4HI GCNGC 8 cut(s) 51, 117, 237, 240, 402, 412, 630, 666
FspBI CTAG 6 cut(s) 299, 492, 669, 1119, 1250, 1284
GluI GCNGC 8 cut(s) 51, 117, 237, 240, 402, 412, 630, 666
GsaI CCCAGC 2 cut(s) 923, 1404
HaeIII GGCC 5 cut(s) 335, 359, 472, 803, 1063
HgaI GACGC 2 cut(s) 1354, 1394
HincII GTYRAC 2 cut(s) 736, 988
HindII GTYRAC 2 cut(s) 736, 988
HinfI GANTC 2 cut(s) 830, 1307
HphI GGTGA 1 cut(s) 691
Hpy166II GTNNAC 4 cut(s) 9, 736, 988, 1153
Hpy188I TCNGA 9 cut(s) 104, 150, 595, 622, 1078, 1242, 1256, 1306, 1344
Hpy188III TCNNGA 4 cut(s) 154, 749, 814, 1214
Hpy8I GTNNAC 4 cut(s) 9, 736, 988, 1153
Hpy99I CGWCG 1 cut(s) 1388
HpyAV CCTTC 8 cut(s) 8, 297, 349, 676, 887, 1087, 1100, 1201
HpyCH4III ACNGT 3 cut(s) 100, 1160, 1232
HpyCH4IV ACGT 1 cut(s) 826
HpyF10VI GCNNNNNNNGC 5 cut(s) 341, 662, 793, 908, 1345
HpyF3I CTNAG 2 cut(s) 619, 797
HpySE526I ACGT 1 cut(s) 826
Ksp22I TGATCA 2 cut(s) 57, 150
Kzo9I GATC 5 cut(s) 57, 150, 210, 1259, 1360
LmnI GCTCC 2 cut(s) 321, 391
Lsp1109I GCAGC 6 cut(s) 37, 248, 251, 388, 641, 652
LweI GCATC 5 cut(s) 598, 609, 1087, 1194, 1310
MaeI CTAG 6 cut(s) 299, 492, 669, 1119, 1250, 1284
MaeII ACGT 1 cut(s) 826
MaeIII GTNAC 1 cut(s) 822
MalI GATC 5 cut(s) 59, 152, 212, 1261, 1362
MboI GATC 5 cut(s) 57, 150, 210, 1259, 1360
MflI RGATCY 2 cut(s) 1259, 1360
MhlI GDGCHC 2 cut(s) 225, 805
MlsI TGGCCA 1 cut(s) 472
MluCI AATT 3 cut(s) 181, 585, 1317
MluI ACGCGT 1 cut(s) 1346
MluNI TGGCCA 1 cut(s) 472
MlyI GAGTC 1 cut(s) 1316
MmeI TCCRAC 1 cut(s) 1287
Mox20I TGGCCA 1 cut(s) 472
Mph1103I ATGCAT 1 cut(s) 1209
MroXI GAANNNNTTC 1 cut(s) 184
MscI TGGCCA 1 cut(s) 472
MseI TTAA 4 cut(s) 266, 692, 707, 1411
Msp20I TGGCCA 1 cut(s) 472
MspA1I CMGCKG 2 cut(s) 656, 863
MspR9I CCNGG 4 cut(s) 127, 177, 292, 474
Mva1269I GAATGC 2 cut(s) 1207, 1223
MvaI CCWGG 4 cut(s) 127, 177, 292, 474
MvnI CGCG 1 cut(s) 1348
MwoI GCNNNNNNNGC 5 cut(s) 341, 662, 793, 908, 1345
NcoI CCATGG 1 cut(s) 1101
NdeI CATATG 1 cut(s) 541
NdeII GATC 5 cut(s) 57, 150, 210, 1259, 1360
NlaIV GGNNCC 4 cut(s) 42, 317, 803, 1027
NmuCI GTSAC 1 cut(s) 822
NsiI ATGCAT 1 cut(s) 1209
NspI RCATGY 1 cut(s) 116
PaeI GCATGC 1 cut(s) 116
PasI CCCWGGG 1 cut(s) 291
PctI GAATGC 2 cut(s) 1207, 1223
PdmI GAANNNNTTC 1 cut(s) 184
PfeI GAWTC 1 cut(s) 830
PfoI TCCNGGA 1 cut(s) 125
PkrI GCNGC 8 cut(s) 52, 118, 238, 241, 403, 413, 631, 667
PleI GAGTC 1 cut(s) 1315
PmaCI CACGTG 1 cut(s) 827
PmlI CACGTG 1 cut(s) 827
PpsI GAGTC 1 cut(s) 1315
Ppu21I YACGTR 1 cut(s) 827
Psp6I CCWGG 4 cut(s) 125, 175, 290, 472
PspCI CACGTG 1 cut(s) 827
PspFI CCCAGC 2 cut(s) 919, 1400
PspGI CCWGG 4 cut(s) 125, 175, 290, 472
PspN4I GGNNCC 4 cut(s) 42, 317, 803, 1027
PspOMI GGGCCC 1 cut(s) 801
PspPI GGNCC 5 cut(s) 333, 455, 801, 802, 1061
PsuI RGATCY 2 cut(s) 1259, 1360
PvuII CAGCTG 1 cut(s) 863
RsaI GTAC 3 cut(s) 762, 1229, 1282
RsaNI GTAC 3 cut(s) 761, 1228, 1281
SaqAI TTAA 4 cut(s) 266, 692, 707, 1411
SatI GCNGC 8 cut(s) 51, 117, 237, 240, 402, 412, 630, 666
Sau3AI GATC 5 cut(s) 57, 150, 210, 1259, 1360
Sau96I GGNCC 5 cut(s) 333, 455, 801, 802, 1061
SchI GAGTC 1 cut(s) 1316
ScrFI CCNGG 4 cut(s) 127, 177, 292, 474
SduI GDGCHC 2 cut(s) 225, 805
SfaNI GCATC 5 cut(s) 598, 609, 1087, 1194, 1310
SfcI CTRYAG 1 cut(s) 9
SinI GGWCC 1 cut(s) 455
SphI GCATGC 1 cut(s) 116
Sse9I AATT 3 cut(s) 181, 585, 1317
SsiI CCGC 4 cut(s) 116, 411, 654, 1122
SspMI CTAG 6 cut(s) 299, 492, 669, 1119, 1250, 1284
StyD4I CCNGG 4 cut(s) 125, 175, 290, 472
StyI CCWWGG 3 cut(s) 336, 1052, 1101
TaaI ACNGT 3 cut(s) 100, 1160, 1232
TaiI ACGT 1 cut(s) 829
TaqI TCGA 4 cut(s) 463, 497, 1383, 1391
TasI AATT 3 cut(s) 181, 585, 1317
TatI WGTACW 1 cut(s) 1227
TauI GCSGC 2 cut(s) 119, 414
TfiI GAWTC 1 cut(s) 830
Tru1I TTAA 4 cut(s) 266, 692, 707, 1411
Tru9I TTAA 4 cut(s) 266, 692, 707, 1411
TseFI GTSAC 1 cut(s) 822
TseI GCWGC 6 cut(s) 50, 236, 239, 401, 629, 665
Tsp45I GTSAC 1 cut(s) 822
TspDTI ATGAA 5 cut(s) 86, 177, 514, 1007, 1389
VpaK11BI GGWCC 1 cut(s) 455
XagI CCTNNNNNAGG 1 cut(s) 1056
XapI RAATTY 1 cut(s) 181
XceI RCATGY 1 cut(s) 116
XmiI GTMKAC 2 cut(s) 8, 1152
XmnI GAANNNNTTC 1 cut(s) 184
XspI CTAG 6 cut(s) 299, 492, 669, 1119, 1250, 1284
Zsp2I ATGCAT 1 cut(s) 1209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.