Rw4G029050

protein phosphatase 2C 15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0002720
Physical Location & Seq
Forward (+)
55729901 .. 55739650
9750 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G029050.1

Sequence Viewer

Length: 1266 bp
ATGGGGTCTACAGAAGGGAGGCGTAATCATCATAACCTGGTGCCTCTTGCTGCATTGATCAGCAGAGAGATGAAGAATGAGAAGATGGAAAAGCCGACTGTCAGATACGGGCATGCGGCTCAATCCAGGAAGGGGGAGGATTATTTTCTGATCAAGACTGATGGTCAGCGAGTGCCTGGAAATTCTTCATCAACATTTTCTGCATTTGCGATCTTTGACGGGCACAATGGAAATGCAGCAACCATATATACAAGGGAGAACTTGTTAAATCATGTGTTGGGTGCTATTCCCAGGGGGCTAGGAAGGGAGGAGTGGCTCCAAGCCTTACCTTGGGCCTTGGTTGCAGGGTTTGTGAAGGCTGATAAGGATTTCCAAAGTAGAGGAGAAACTTCTGGAACAACACCTACTGTTGTAATAGTTGATGGATGGACTGTGACAGTAGCATCAGTAGGAGACTCCCGTTGTACCTTAGATACTCAGGGTGGTGCAGTTTCCACTTTAATTGTTGATCATAGACTTGAAGAGAATGTAGAAGACGTGTTACTGCAAGTGGTGGGGAGGTTGAGCATTGTTGGTGGTGCGGAGATTGGTCCTCTTCGTTGTTGGCCAGGAGGTTTATGCCTGTCTAGGTCGATTGGAGACATGGATGTTGGGGAGTTCATAGTTCCAATACCATATGTGAAACAAGTCAAGCTATCAAATAGAGGAGGGAGGCTCATAATTGCTTCTTATGGCATCTGGGATGCCCTATCCTCAGAAGAGGCAGCAAAATCTTGCCGTGGGTTGCCCGCTGAACTTGCTGCTAGACAAGTAGTGAAGGAGGCTTTAAGGTCAAGGGGTTTAAAGGGTGATACAACCTGTATAGTTGTTGACATAATCCCTCCTGATAACTCGGTACTGCCTACAACTCCCCCAAAAAAGCAGAACAAGCTGAGGGCCCTGTTGTTCAGGAAGAAGTCACGTGATTCTGCTCACAAACTATCCAAGAAGCTATCAGCTGTGGGTATTGTGGAAGAACTATTTGAAGAAGGCTCTGCCATGCTTGCTGAAAGGCTGGGCAATGATGAGTGCACGGCACAATCAACAACATCTGGGCTGTTTATGTGTGCTGTATGCCAAGTTGACCTTGCACCGAGTGAGGGCATATCTGTTCATGCTGGTTCCATGTGGGGCCTTGGCAAGGGCCTTTCCTCTGTTCTGATTGTCGTAATAATTAAGAAGGATGCCATGGAAGGAAAACGCCCTAGCGGAGTAAGAGTAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

421

Amino Acids

45.24

Weight (kDa)

8.7

Isoelectric Point (pI)

34.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 61 - 277 2.9e-23 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 40
AccI GTMKAC 1 cut(s) 8
AciI CCGC 4 cut(s) 116, 581, 789, 1248
AcoI YGGCCR 1 cut(s) 605
AcsI RAATTY 1 cut(s) 181
AcvI CACGTG 1 cut(s) 962
AdeI CACNNNGTG 1 cut(s) 1136
AfaI GTAC 2 cut(s) 466, 897
AfiI CCNNNNNNNGG 4 cut(s) 132, 330, 1139, 1180
AflIII ACRYGT 1 cut(s) 537
AgsI TTSAA 2 cut(s) 521, 1025
AhdI GACNNNNNGTC 1 cut(s) 162
AjiI CACGTC 1 cut(s) 538
AjnI CCWGG 5 cut(s) 36, 125, 175, 290, 607
AluBI AGCT 4 cut(s) 694, 931, 991, 998
AluI AGCT 4 cut(s) 694, 931, 991, 998
Alw21I GWGCWC 1 cut(s) 1073
Alw26I GTCTC 2 cut(s) 447, 633
Alw44I GTGCAC 1 cut(s) 1069
AoxI GGCC 5 cut(s) 333, 605, 936, 1171, 1183
ApaI GGGCCC 1 cut(s) 940
ApaLI GTGCAC 1 cut(s) 1069
ApeKI GCWGC 4 cut(s) 50, 236, 764, 800
ApoI RAATTY 1 cut(s) 181
Asp700I GAANNNNTTC 1 cut(s) 184
AspS9I GGNCC 6 cut(s) 333, 590, 936, 937, 1171, 1183
AsuHPI GGTGA 1 cut(s) 860
AvaII GGWCC 1 cut(s) 590
BaeGI GKGCMC 3 cut(s) 225, 940, 1073
BalI TGGCCA 1 cut(s) 607
BanI GGYRCC 1 cut(s) 40
BanII GRGCYC 1 cut(s) 940
BarI GAAGNNNNNNTAC 2 cut(s) 525, 557
BbrPI CACGTG 1 cut(s) 962
BbsI GAAGAC 1 cut(s) 540
Bbv12I GWGCWC 1 cut(s) 1073
BbvCI CCTCAGC 1 cut(s) 932
BbvI GCAGC 4 cut(s) 37, 248, 776, 787
BccI CCATC 4 cut(s) 79, 155, 416, 420
BceAI ACGGC 2 cut(s) 762, 1089
BciT130I CCWGG 5 cut(s) 38, 127, 177, 292, 609
BclI TGATCA 3 cut(s) 57, 150, 508
BcoDI GTCTC 2 cut(s) 447, 633
BfaI CTAG 4 cut(s) 299, 627, 804, 1245
BfmI CTRYAG 1 cut(s) 9
BisI GCNGC 5 cut(s) 51, 117, 237, 765, 801
BlsI GCNGC 5 cut(s) 52, 118, 238, 766, 802
Bme1390I CCNGG 5 cut(s) 38, 127, 177, 292, 609
Bme18I GGWCC 1 cut(s) 590
BmeRI GACNNNNNGTC 1 cut(s) 162
BmgBI CACGTC 1 cut(s) 538
BmgT120I GGNCC 6 cut(s) 333, 590, 936, 937, 1171, 1183
BmiI GGNNCC 5 cut(s) 42, 317, 938, 1162, 1172
BmrFI CCNGG 5 cut(s) 38, 127, 177, 292, 609
BmsI GCATC 4 cut(s) 452, 733, 744, 1213
BpiI GAAGAC 1 cut(s) 540
BplI GAGNNNNNCTC 2 cut(s) 699, 731
Bpu10I CCTNAGC 1 cut(s) 932
BsaAI YACGTR 1 cut(s) 962
BsaJI CCNNGG 7 cut(s) 290, 291, 329, 336, 778, 1174, 1227
BsaXI ACNNNNNCTCC 3 cut(s) 248, 278, 1242
Bsc4I CCNNNNNNNGG 4 cut(s) 132, 330, 1139, 1180
Bse3DI GCAATG 1 cut(s) 1066
BseBI CCWGG 5 cut(s) 38, 127, 177, 292, 609
BseDI CCNNGG 7 cut(s) 290, 291, 329, 336, 778, 1174, 1227
BseGI GGATG 4 cut(s) 431, 652, 748, 1228
BseLI CCNNNNNNNGG 4 cut(s) 132, 330, 1139, 1180
BseMI GCAATG 1 cut(s) 1066
BseMII CTCAG 3 cut(s) 491, 768, 923
BseRI GAGGAG 3 cut(s) 323, 396, 720
BseSI GKGCMC 3 cut(s) 225, 940, 1073
BseXI GCAGC 4 cut(s) 37, 248, 776, 787
BseYI CCCAGC 1 cut(s) 1054
BsgI GTGCAG 1 cut(s) 507
BshFI GGCC 5 cut(s) 335, 607, 938, 1173, 1185
BshNI GGYRCC 1 cut(s) 40
BsiHKAI GWGCWC 1 cut(s) 1073
BslI CCNNNNNNNGG 4 cut(s) 132, 330, 1139, 1180
BsmAI GTCTC 2 cut(s) 447, 633
BsnI GGCC 5 cut(s) 335, 607, 938, 1173, 1185
Bsp120I GGGCCC 1 cut(s) 936
Bsp1286I GDGCHC 3 cut(s) 225, 940, 1073
Bsp143I GATC 4 cut(s) 57, 150, 210, 508
Bsp19I CCATGG 1 cut(s) 1227
BspACI CCGC 4 cut(s) 116, 581, 789, 1248
BspANI GGCC 5 cut(s) 335, 607, 938, 1173, 1185
BspCNI CTCAG 3 cut(s) 490, 767, 924
BspLI GGNNCC 5 cut(s) 42, 317, 938, 1162, 1172
BspT107I GGYRCC 1 cut(s) 40
BsrDI GCAATG 1 cut(s) 1066
BssECI CCNNGG 7 cut(s) 290, 291, 329, 336, 778, 1174, 1227
BssMI GATC 4 cut(s) 57, 150, 210, 508
BssT1I CCWWGG 4 cut(s) 329, 336, 1174, 1227
Bst2UI CCWGG 5 cut(s) 38, 127, 177, 292, 609
Bst4CI ACNGT 4 cut(s) 100, 409, 433, 439
Bst6I CTCTTC 3 cut(s) 516, 600, 753
BstBAI YACGTR 1 cut(s) 962
BstC8I GCNNGC 3 cut(s) 114, 789, 1044
BstDEI CTNAG 4 cut(s) 469, 477, 754, 932
BstDSI CCRYGG 2 cut(s) 778, 1227
BstENI CCTNNNNNAGG 1 cut(s) 1178
BstF5I GGATG 4 cut(s) 431, 652, 748, 1228
BstKTI GATC 4 cut(s) 60, 153, 213, 511
BstMAI GTCTC 2 cut(s) 447, 633
BstMBI GATC 4 cut(s) 57, 150, 210, 508
BstMWI GCNNNNNNNGC 4 cut(s) 341, 797, 928, 1043
BstNI CCWGG 5 cut(s) 38, 127, 177, 292, 609
BstNSI RCATGY 1 cut(s) 116
BstSCI CCNGG 5 cut(s) 36, 125, 175, 290, 607
BstSFI CTRYAG 1 cut(s) 9
BstSLI GKGCMC 3 cut(s) 225, 940, 1073
BstV1I GCAGC 4 cut(s) 37, 248, 776, 787
BstV2I GAAGAC 1 cut(s) 540
BsuRI GGCC 5 cut(s) 335, 607, 938, 1173, 1185
BtgI CCRYGG 2 cut(s) 778, 1227
BtrI CACGTC 1 cut(s) 538
BtsCI GGATG 4 cut(s) 431, 652, 748, 1228
Cac8I GCNNGC 3 cut(s) 114, 789, 1044
Cfr13I GGNCC 6 cut(s) 333, 590, 936, 937, 1171, 1183
CsiI ACCWGGT 1 cut(s) 36
Csp6I GTAC 2 cut(s) 465, 896
CspCI CAANNNNNGTGG 2 cut(s) 484, 519
CviAII CATG 7 cut(s) 113, 272, 643, 1039, 1154, 1165, 1228
CviQI GTAC 2 cut(s) 465, 896
DdeI CTNAG 4 cut(s) 469, 477, 754, 932
DpnI GATC 4 cut(s) 59, 152, 212, 510
DpnII GATC 4 cut(s) 57, 150, 210, 508
DraI TTTAAA 1 cut(s) 843
DraIII CACNNNGTG 1 cut(s) 1136
DriI GACNNNNNGTC 1 cut(s) 162
EaeI YGGCCR 1 cut(s) 605
Eam1104I CTCTTC 3 cut(s) 516, 600, 753
Eam1105I GACNNNNNGTC 1 cut(s) 162
EarI CTCTTC 3 cut(s) 516, 600, 753
Eco130I CCWWGG 4 cut(s) 329, 336, 1174, 1227
Eco24I GRGCYC 1 cut(s) 940
Eco47I GGWCC 1 cut(s) 590
Eco72I CACGTG 1 cut(s) 962
EcoNI CCTNNNNNAGG 1 cut(s) 1178
EcoO109I RGGNCCY 4 cut(s) 936, 937, 1171, 1183
EcoRII CCWGG 5 cut(s) 36, 125, 175, 290, 607
EcoT14I CCWWGG 4 cut(s) 329, 336, 1174, 1227
EcoT38I GRGCYC 1 cut(s) 940
ErhI CCWWGG 4 cut(s) 329, 336, 1174, 1227
FaeI CATG 7 cut(s) 116, 275, 646, 1042, 1157, 1168, 1231
FalI AAGNNNNNCTT 2 cut(s) 1110, 1142
FatI CATG 7 cut(s) 112, 271, 642, 1038, 1153, 1164, 1227
FauI CCCGC 1 cut(s) 796
FauNDI CATATG 1 cut(s) 676
FbaI TGATCA 3 cut(s) 57, 150, 508
FblI GTMKAC 1 cut(s) 8
Fnu4HI GCNGC 5 cut(s) 51, 117, 237, 765, 801
FokI GGATG 4 cut(s) 438, 659, 755, 1235
FriOI GRGCYC 1 cut(s) 940
Fsp4HI GCNGC 5 cut(s) 51, 117, 237, 765, 801
FspBI CTAG 4 cut(s) 299, 627, 804, 1245
GluI GCNGC 5 cut(s) 51, 117, 237, 765, 801
GsaI CCCAGC 1 cut(s) 1058
HaeIII GGCC 5 cut(s) 335, 607, 938, 1173, 1185
Hin1II CATG 7 cut(s) 116, 275, 646, 1042, 1157, 1168, 1231
HincII GTYRAC 2 cut(s) 871, 1123
HindII GTYRAC 2 cut(s) 871, 1123
HinfI GANTC 2 cut(s) 455, 965
HphI GGTGA 1 cut(s) 860
Hpy166II GTNNAC 4 cut(s) 9, 871, 1071, 1123
Hpy188I TCNGA 4 cut(s) 104, 150, 757, 1200
Hpy188III TCNNGA 4 cut(s) 154, 393, 884, 949
Hpy8I GTNNAC 4 cut(s) 9, 871, 1071, 1123
HpyAV CCTTC 8 cut(s) 8, 124, 297, 349, 811, 1022, 1213, 1226
HpyCH4III ACNGT 4 cut(s) 100, 409, 433, 439
HpyCH4IV ACGT 2 cut(s) 537, 961
HpyCH4V TGCA 8 cut(s) 53, 203, 236, 344, 488, 547, 1071, 1130
HpyF10VI GCNNNNNNNGC 4 cut(s) 341, 797, 928, 1043
HpyF3I CTNAG 4 cut(s) 469, 477, 754, 932
HpySE526I ACGT 2 cut(s) 537, 961
Hsp92II CATG 7 cut(s) 116, 275, 646, 1042, 1157, 1168, 1231
Ksp22I TGATCA 3 cut(s) 57, 150, 508
Kzo9I GATC 4 cut(s) 57, 150, 210, 508
LmnI GCTCC 1 cut(s) 321
Lsp1109I GCAGC 4 cut(s) 37, 248, 776, 787
LweI GCATC 4 cut(s) 452, 733, 744, 1213
MabI ACCWGGT 1 cut(s) 36
MaeI CTAG 4 cut(s) 299, 627, 804, 1245
MaeII ACGT 2 cut(s) 537, 961
MaeIII GTNAC 3 cut(s) 433, 540, 957
MalI GATC 4 cut(s) 59, 152, 212, 510
MboI GATC 4 cut(s) 57, 150, 210, 508
MhlI GDGCHC 3 cut(s) 225, 940, 1073
MlsI TGGCCA 1 cut(s) 607
MluCI AATT 4 cut(s) 181, 501, 720, 1212
MluNI TGGCCA 1 cut(s) 607
MlyI GAGTC 1 cut(s) 449
Mox20I TGGCCA 1 cut(s) 607
MroXI GAANNNNTTC 1 cut(s) 184
MscI TGGCCA 1 cut(s) 607
MseI TTAA 5 cut(s) 266, 500, 827, 842, 1215
Msp20I TGGCCA 1 cut(s) 607
MspA1I CMGCKG 2 cut(s) 791, 998
MspR9I CCNGG 5 cut(s) 38, 127, 177, 292, 609
MvaI CCWGG 5 cut(s) 38, 127, 177, 292, 609
MwoI GCNNNNNNNGC 4 cut(s) 341, 797, 928, 1043
NcoI CCATGG 1 cut(s) 1227
NdeI CATATG 1 cut(s) 676
NdeII GATC 4 cut(s) 57, 150, 210, 508
NlaIII CATG 7 cut(s) 116, 275, 646, 1042, 1157, 1168, 1231
NlaIV GGNNCC 5 cut(s) 42, 317, 938, 1162, 1172
NmuCI GTSAC 2 cut(s) 433, 957
NspI RCATGY 1 cut(s) 116
PaeI GCATGC 1 cut(s) 116
PasI CCCWGGG 1 cut(s) 291
PdmI GAANNNNTTC 1 cut(s) 184
PfeI GAWTC 1 cut(s) 965
PfoI TCCNGGA 1 cut(s) 125
PkrI GCNGC 5 cut(s) 52, 118, 238, 766, 802
PleI GAGTC 1 cut(s) 449
PmaCI CACGTG 1 cut(s) 962
PmlI CACGTG 1 cut(s) 962
PpsI GAGTC 1 cut(s) 449
Ppu21I YACGTR 1 cut(s) 962
Psp6I CCWGG 5 cut(s) 36, 125, 175, 290, 607
PspCI CACGTG 1 cut(s) 962
PspFI CCCAGC 1 cut(s) 1054
PspGI CCWGG 5 cut(s) 36, 125, 175, 290, 607
PspN4I GGNNCC 5 cut(s) 42, 317, 938, 1162, 1172
PspOMI GGGCCC 1 cut(s) 936
PspPI GGNCC 6 cut(s) 333, 590, 936, 937, 1171, 1183
PsrI GAACNNNNNNTAC 2 cut(s) 388, 420
PvuII CAGCTG 1 cut(s) 998
RsaI GTAC 2 cut(s) 466, 897
RsaNI GTAC 2 cut(s) 465, 896
SaqAI TTAA 5 cut(s) 266, 500, 827, 842, 1215
SatI GCNGC 5 cut(s) 51, 117, 237, 765, 801
Sau3AI GATC 4 cut(s) 57, 150, 210, 508
Sau96I GGNCC 6 cut(s) 333, 590, 936, 937, 1171, 1183
SchI GAGTC 1 cut(s) 449
ScrFI CCNGG 5 cut(s) 38, 127, 177, 292, 609
SduI GDGCHC 3 cut(s) 225, 940, 1073
SexAI ACCWGGT 1 cut(s) 36
SfaNI GCATC 4 cut(s) 452, 733, 744, 1213
SfcI CTRYAG 1 cut(s) 9
SinI GGWCC 1 cut(s) 590
SphI GCATGC 1 cut(s) 116
Sse9I AATT 4 cut(s) 181, 501, 720, 1212
SsiI CCGC 4 cut(s) 116, 581, 789, 1248
SspMI CTAG 4 cut(s) 299, 627, 804, 1245
StyD4I CCNGG 5 cut(s) 36, 125, 175, 290, 607
StyI CCWWGG 4 cut(s) 329, 336, 1174, 1227
TaaI ACNGT 4 cut(s) 100, 409, 433, 439
TaiI ACGT 2 cut(s) 540, 964
TaqI TCGA 1 cut(s) 632
TasI AATT 4 cut(s) 181, 501, 720, 1212
TauI GCSGC 1 cut(s) 119
TfiI GAWTC 1 cut(s) 965
Tru1I TTAA 5 cut(s) 266, 500, 827, 842, 1215
Tru9I TTAA 5 cut(s) 266, 500, 827, 842, 1215
TseFI GTSAC 2 cut(s) 433, 957
TseI GCWGC 4 cut(s) 50, 236, 764, 800
Tsp45I GTSAC 2 cut(s) 433, 957
TspDTI ATGAA 4 cut(s) 86, 177, 649, 1142
VneI GTGCAC 1 cut(s) 1069
VpaK11BI GGWCC 1 cut(s) 590
XagI CCTNNNNNAGG 1 cut(s) 1178
XapI RAATTY 1 cut(s) 181
XceI RCATGY 1 cut(s) 116
XmiI GTMKAC 1 cut(s) 8
XmnI GAANNNNTTC 1 cut(s) 184
XspI CTAG 4 cut(s) 299, 627, 804, 1245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.