MD16G1039100.v1.1

protein phosphatase 2C 15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0002720
Physical Location & Seq
Forward (+)
2763789 .. 2767458
3670 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1039100.v1.1.491

Sequence Viewer

Length: 1287 bp
ATGGCGTCCGGAGATAGGCGTCAGCATCATAATATGGTGCCTCTGGCTGCATTGATCAGCAGAGAGCTGAAGAGTGAGAAGATGGAGAAGCCAACTGTCAGATATGGGCATGCAGCTCAATCCAGGAAGGGGGAGGATTATTTCCTGATCAAGACTGATGGTCAGCGAGTACCCGGGAATTCTTCATCAGCATTCTCCGCATTTGCGATCTTTGATGGGCACAATGGAAATGCAGCAGCAGTATATACAAGGGAGAATTTGTTAAAGCATGTGTTGGGTGCTATTCCCCGTGGGCTAGGAAGGGAAGAGTGGCTCCAAGCTTTACCTCGAGCCTTGGTTGCTGGCTTTGTGAAGACTGATAAGGATTTCCAGAGCAGAGGTGAAACTTCTGGAACAACAGCTACCTTTGTAATAGTTGATGGATGGACCGTGACAGTTGCATCAGTAGGAGACTCCCGTTGTATCTTAGATACTCAGGGTGGTGCTGTTTCCACCTTAACTGTTGATCATAGACTTGAAGAAAATGTAGAGGAGAGGGAGCGTGTTGCTGCAAGTGGTGGGGAAGTTGGGAGGTTGAGCATTGTTGGTGGTGCAGAGATTGGACCCCTCCGTTGTTGGCCAGGGGGTTTATGCCTTTCTAGATCAATTGGAGACATGGATGTTGGCGAGTTTATAGTTCCAATACCATATGTGAAACAAGTCAAGCTTTCAAATGCTGGTGGGAGGCTTATTATTGCTTCTGATGGCATCTGGGATGCTATATCTTCCGAAATGGCAGCACATTCTTGCCGTGGGTTGCCAGCTGAACTTGCTGCCAGACAAGTTGTGAAGGAGGCGTTAAGGTCTAGGGGTCTAAAGGATGATACAACCTGCATAGTTGTTGACATAATCCCTCCTGATATTACCGAACAGCCTTCAACTCCGCCAAAGAAGATTAACAAGCTTAGGACCCTGTTGTTCAGAAAGAAGTCGCGTGATTCTGCTAATAAACTATCCAAGAAGCTATCGGCTGTGGGTATTGTGGAAGAGTTGTTTGAAGAAGGGTCTGCGATGCTTGCTGAAAGGTTGGGCAGTGATGAGTGCACACCACAATCGACAACATCTGGGCTATTCATGTGTGCTGTATGCCAGGTTGACCTTGCTCCGAGCGAGGGCATATCGGTTCACGCAGGTTCTATCTTCTCAACCAGTTCAAAGCCTTGGCAAGGGCCGTTCCTCTGTTCTGATTGTCGTAACAAGAAGGATGCCATGGAAGGAAAACGCCCTAGCGGAGTCAGAGTAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

429

Amino Acids

45.99

Weight (kDa)

8.27

Isoelectric Point (pI)

37.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 60 - 281 7.6e-35 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 878, 1160
AccB1I GGYRCC 1 cut(s) 37
AccII CGCG 1 cut(s) 973
AccIII TCCGGA 1 cut(s) 8
AciI CCGC 3 cut(s) 198, 923, 1269
AcoI YGGCCR 1 cut(s) 617
AcsI RAATTY 2 cut(s) 178, 256
AcuI CTGAAG 1 cut(s) 89
AcyI GRCGYC 2 cut(s) 5, 19
AfaI GTAC 1 cut(s) 171
AfiI CCNNNNNNNGG 4 cut(s) 15, 129, 1151, 1205
AgsI TTSAA 5 cut(s) 518, 711, 918, 1037, 1194
AhdI GACNNNNNGTC 1 cut(s) 159
AjnI CCWGG 3 cut(s) 122, 619, 1128
AluBI AGCT 8 cut(s) 67, 116, 320, 401, 706, 803, 943, 1003
AluI AGCT 8 cut(s) 67, 116, 320, 401, 706, 803, 943, 1003
Alw21I GWGCWC 1 cut(s) 1085
Alw26I GTCTC 2 cut(s) 444, 645
Alw44I GTGCAC 1 cut(s) 1081
Ama87I CYCGRG 2 cut(s) 173, 327
Aor13HI TCCGGA 1 cut(s) 8
AoxI GGCC 2 cut(s) 617, 1208
ApaLI GTGCAC 1 cut(s) 1081
ApeKI GCWGC 7 cut(s) 47, 113, 233, 236, 548, 776, 812
ApoI RAATTY 2 cut(s) 178, 256
AspS9I GGNCC 4 cut(s) 426, 602, 948, 1208
AsuC2I CCSGG 2 cut(s) 174, 175
AsuHPI GGTGA 1 cut(s) 392
AvaI CYCGRG 2 cut(s) 173, 327
AvaII GGWCC 3 cut(s) 426, 602, 948
BaeGI GKGCMC 2 cut(s) 222, 1085
BalI TGGCCA 1 cut(s) 619
BanI GGYRCC 1 cut(s) 37
BbsI GAAGAC 1 cut(s) 359
Bbv12I GWGCWC 1 cut(s) 1085
BbvI GCAGC 7 cut(s) 34, 125, 245, 248, 535, 788, 799
BccI CCATC 6 cut(s) 76, 152, 209, 413, 417, 737
BceAI ACGGC 2 cut(s) 774, 1195
BciT130I CCWGG 3 cut(s) 124, 621, 1130
BclI TGATCA 3 cut(s) 54, 147, 505
BcnI CCSGG 2 cut(s) 174, 175
BcoDI GTCTC 2 cut(s) 444, 645
BfaI CTAG 4 cut(s) 296, 639, 846, 1266
BfuAI ACCTGC 2 cut(s) 878, 1160
BisI GCNGC 7 cut(s) 48, 114, 234, 237, 549, 777, 813
BlsI GCNGC 7 cut(s) 49, 115, 235, 238, 550, 778, 814
Bme1390I CCNGG 5 cut(s) 124, 174, 175, 621, 1130
Bme18I GGWCC 3 cut(s) 426, 602, 948
BmeRI GACNNNNNGTC 1 cut(s) 159
BmeT110I CYCGRG 2 cut(s) 173, 327
BmgT120I GGNCC 4 cut(s) 426, 602, 948, 1208
BmiI GGNNCC 4 cut(s) 39, 314, 604, 950
BmrFI CCNGG 5 cut(s) 124, 174, 175, 621, 1130
BmsI GCATC 6 cut(s) 34, 449, 745, 756, 1041, 1234
BpiI GAAGAC 1 cut(s) 359
Bpu10I CCTNAGC 1 cut(s) 944
BpuMI CCSGG 2 cut(s) 174, 175
BsaHI GRCGYC 2 cut(s) 5, 19
BsaJI CCNNGG 7 cut(s) 173, 289, 333, 620, 790, 1199, 1248
BsaWI WCCGGW 1 cut(s) 8
BsaXI ACNNNNNCTCC 3 cut(s) 245, 275, 1263
Bsc4I CCNNNNNNNGG 4 cut(s) 15, 129, 1151, 1205
Bse1I ACTGG 1 cut(s) 1188
BseAI TCCGGA 1 cut(s) 8
BseBI CCWGG 3 cut(s) 124, 621, 1130
BseDI CCNNGG 7 cut(s) 173, 289, 333, 620, 790, 1199, 1248
BseGI GGATG 5 cut(s) 428, 664, 760, 865, 1249
BseLI CCNNNNNNNGG 4 cut(s) 15, 129, 1151, 1205
BseMII CTCAG 1 cut(s) 488
BseNI ACTGG 1 cut(s) 1188
BseRI GAGGAG 1 cut(s) 545
BseSI GKGCMC 2 cut(s) 222, 1085
BseXI GCAGC 7 cut(s) 34, 125, 245, 248, 535, 788, 799
BsgI GTGCAG 1 cut(s) 612
Bsh1236I CGCG 1 cut(s) 973
BshFI GGCC 2 cut(s) 619, 1210
BshNI GGYRCC 1 cut(s) 37
BsiHKAI GWGCWC 1 cut(s) 1085
BsiHKCI CYCGRG 2 cut(s) 173, 327
BsiSI CCGG 2 cut(s) 9, 174
BslI CCNNNNNNNGG 4 cut(s) 15, 129, 1151, 1205
BsmAI GTCTC 2 cut(s) 444, 645
BsmI GAATGC 1 cut(s) 191
BsnI GGCC 2 cut(s) 619, 1210
BsoBI CYCGRG 2 cut(s) 173, 327
Bsp1286I GDGCHC 2 cut(s) 222, 1085
Bsp13I TCCGGA 1 cut(s) 8
Bsp143I GATC 5 cut(s) 54, 147, 207, 505, 641
Bsp19I CCATGG 1 cut(s) 1248
BspACI CCGC 3 cut(s) 198, 923, 1269
BspANI GGCC 2 cut(s) 619, 1210
BspCNI CTCAG 1 cut(s) 487
BspEI TCCGGA 1 cut(s) 8
BspFNI CGCG 1 cut(s) 973
BspLI GGNNCC 4 cut(s) 39, 314, 604, 950
BspMI ACCTGC 2 cut(s) 878, 1160
BspT107I GGYRCC 1 cut(s) 37
BsrI ACTGG 1 cut(s) 1188
BssECI CCNNGG 7 cut(s) 173, 289, 333, 620, 790, 1199, 1248
BssMI GATC 5 cut(s) 54, 147, 207, 505, 641
BssNI GRCGYC 2 cut(s) 5, 19
BssT1I CCWWGG 3 cut(s) 333, 1199, 1248
Bst2UI CCWGG 3 cut(s) 124, 621, 1130
Bst4CI ACNGT 4 cut(s) 97, 430, 436, 502
Bst6I CTCTTC 3 cut(s) 65, 300, 1020
BstACI GRCGYC 2 cut(s) 5, 19
BstC8I GCNNGC 4 cut(s) 111, 343, 801, 1056
BstDEI CTNAG 3 cut(s) 466, 474, 944
BstDSI CCRYGG 3 cut(s) 289, 790, 1248
BstENI CCTNNNNNAGG 1 cut(s) 1203
BstF5I GGATG 5 cut(s) 428, 664, 760, 865, 1249
BstFNI CGCG 1 cut(s) 973
BstKTI GATC 5 cut(s) 57, 150, 210, 508, 644
BstMAI GTCTC 2 cut(s) 444, 645
BstMBI GATC 5 cut(s) 54, 147, 207, 505, 641
BstMWI GCNNNNNNNGC 4 cut(s) 197, 338, 809, 1055
BstNI CCWGG 3 cut(s) 124, 621, 1130
BstNSI RCATGY 2 cut(s) 113, 272
BstSCI CCNGG 5 cut(s) 122, 172, 173, 619, 1128
BstSLI GKGCMC 2 cut(s) 222, 1085
BstUI CGCG 1 cut(s) 973
BstV1I GCAGC 7 cut(s) 34, 125, 245, 248, 535, 788, 799
BstV2I GAAGAC 1 cut(s) 359
BsuRI GGCC 2 cut(s) 619, 1210
BtgI CCRYGG 3 cut(s) 289, 790, 1248
BtgZI GCGATG 1 cut(s) 1064
BtsCI GGATG 5 cut(s) 428, 664, 760, 865, 1249
BtsI GCAGTG 1 cut(s) 1078
BtsIMutI CAGTG 1 cut(s) 1078
BveI ACCTGC 2 cut(s) 878, 1160
Cac8I GCNNGC 4 cut(s) 111, 343, 801, 1056
Cfr13I GGNCC 4 cut(s) 426, 602, 948, 1208
Cfr9I CCCGGG 1 cut(s) 173
CseI GACGC 1 cut(s) 8
Csp6I GTAC 1 cut(s) 170
CviAII CATG 5 cut(s) 110, 269, 655, 1114, 1249
CviQI GTAC 1 cut(s) 170
DdeI CTNAG 3 cut(s) 466, 474, 944
DpnI GATC 5 cut(s) 56, 149, 209, 507, 643
DpnII GATC 5 cut(s) 54, 147, 207, 505, 641
DriI GACNNNNNGTC 1 cut(s) 159
EaeI YGGCCR 1 cut(s) 617
Eam1104I CTCTTC 3 cut(s) 65, 300, 1020
Eam1105I GACNNNNNGTC 1 cut(s) 159
EarI CTCTTC 3 cut(s) 65, 300, 1020
EciI GGCGGA 1 cut(s) 912
Eco130I CCWWGG 3 cut(s) 333, 1199, 1248
Eco47I GGWCC 3 cut(s) 426, 602, 948
Eco57I CTGAAG 1 cut(s) 89
Eco88I CYCGRG 2 cut(s) 173, 327
EcoNI CCTNNNNNAGG 1 cut(s) 1203
EcoO109I RGGNCCY 1 cut(s) 948
EcoRI GAATTC 1 cut(s) 178
EcoRII CCWGG 3 cut(s) 122, 619, 1128
EcoT14I CCWWGG 3 cut(s) 333, 1199, 1248
ErhI CCWWGG 3 cut(s) 333, 1199, 1248
FaeI CATG 5 cut(s) 113, 272, 658, 1117, 1252
FalI AAGNNNNNCTT 2 cut(s) 690, 722
FatI CATG 5 cut(s) 109, 268, 654, 1113, 1248
FauNDI CATATG 1 cut(s) 688
FbaI TGATCA 3 cut(s) 54, 147, 505
Fnu4HI GCNGC 7 cut(s) 48, 114, 234, 237, 549, 777, 813
FokI GGATG 5 cut(s) 435, 671, 767, 872, 1256
Fsp4HI GCNGC 7 cut(s) 48, 114, 234, 237, 549, 777, 813
FspBI CTAG 4 cut(s) 296, 639, 846, 1266
GluI GCNGC 7 cut(s) 48, 114, 234, 237, 549, 777, 813
HaeIII GGCC 2 cut(s) 619, 1210
HapII CCGG 2 cut(s) 9, 174
HgaI GACGC 1 cut(s) 8
Hin1I GRCGYC 2 cut(s) 5, 19
Hin1II CATG 5 cut(s) 113, 272, 658, 1117, 1252
HincII GTYRAC 2 cut(s) 883, 1135
HindII GTYRAC 2 cut(s) 883, 1135
HindIII AAGCTT 3 cut(s) 318, 704, 941
HinfI GANTC 3 cut(s) 452, 977, 1272
HpaII CCGG 2 cut(s) 9, 174
HphI GGTGA 1 cut(s) 392
Hpy166II GTNNAC 4 cut(s) 883, 1083, 1135, 1165
Hpy188I TCNGA 7 cut(s) 101, 742, 769, 962, 1146, 1225, 1277
Hpy188III TCNNGA 7 cut(s) 9, 145, 151, 370, 390, 639, 896
Hpy8I GTNNAC 4 cut(s) 883, 1083, 1135, 1165
HpyAV CCTTC 7 cut(s) 121, 294, 823, 924, 1034, 1234, 1247
HpyCH4III ACNGT 4 cut(s) 97, 430, 436, 502
HpyCH4V TGCA 8 cut(s) 50, 113, 233, 440, 551, 593, 873, 1083
HpyF10VI GCNNNNNNNGC 4 cut(s) 197, 338, 809, 1055
HpyF3I CTNAG 3 cut(s) 466, 474, 944
Hsp92I GRCGYC 2 cut(s) 5, 19
Hsp92II CATG 5 cut(s) 113, 272, 658, 1117, 1252
Kpn2I TCCGGA 1 cut(s) 8
Ksp22I TGATCA 3 cut(s) 54, 147, 505
Kzo9I GATC 5 cut(s) 54, 147, 207, 505, 641
LmnI GCTCC 3 cut(s) 318, 538, 1147
Lsp1109I GCAGC 7 cut(s) 34, 125, 245, 248, 535, 788, 799
LweI GCATC 6 cut(s) 34, 449, 745, 756, 1041, 1234
MaeI CTAG 4 cut(s) 296, 639, 846, 1266
MaeIII GTNAC 2 cut(s) 430, 1232
MalI GATC 5 cut(s) 56, 149, 209, 507, 643
MboI GATC 5 cut(s) 54, 147, 207, 505, 641
MfeI CAATTG 1 cut(s) 645
MhlI GDGCHC 2 cut(s) 222, 1085
MlsI TGGCCA 1 cut(s) 619
MluCI AATT 3 cut(s) 178, 256, 645
MluNI TGGCCA 1 cut(s) 619
MlyI GAGTC 2 cut(s) 446, 1281
Mox20I TGGCCA 1 cut(s) 619
MroI TCCGGA 1 cut(s) 8
MscI TGGCCA 1 cut(s) 619
MseI TTAA 4 cut(s) 263, 497, 839, 936
Msp20I TGGCCA 1 cut(s) 619
MspA1I CMGCKG 1 cut(s) 803
MspI CCGG 2 cut(s) 9, 174
MspR9I CCNGG 5 cut(s) 124, 174, 175, 621, 1130
MunI CAATTG 1 cut(s) 645
Mva1269I GAATGC 1 cut(s) 191
MvaI CCWGG 3 cut(s) 124, 621, 1130
MvnI CGCG 1 cut(s) 973
MwoI GCNNNNNNNGC 4 cut(s) 197, 338, 809, 1055
NciI CCSGG 2 cut(s) 174, 175
NcoI CCATGG 1 cut(s) 1248
NdeI CATATG 1 cut(s) 688
NdeII GATC 5 cut(s) 54, 147, 207, 505, 641
NlaIII CATG 5 cut(s) 113, 272, 658, 1117, 1252
NlaIV GGNNCC 4 cut(s) 39, 314, 604, 950
NmuCI GTSAC 1 cut(s) 430
NspI RCATGY 2 cut(s) 113, 272
PaeI GCATGC 1 cut(s) 113
PaeR7I CTCGAG 1 cut(s) 327
PctI GAATGC 1 cut(s) 191
PfeI GAWTC 1 cut(s) 977
PfoI TCCNGGA 1 cut(s) 122
PkrI GCNGC 7 cut(s) 49, 115, 235, 238, 550, 778, 814
PleI GAGTC 2 cut(s) 446, 1280
PpsI GAGTC 2 cut(s) 446, 1280
PpuMI RGGWCCY 1 cut(s) 948
Psp5II RGGWCCY 1 cut(s) 948
Psp6I CCWGG 3 cut(s) 122, 619, 1128
PspGI CCWGG 3 cut(s) 122, 619, 1128
PspN4I GGNNCC 4 cut(s) 39, 314, 604, 950
PspPI GGNCC 4 cut(s) 426, 602, 948, 1208
PspPPI RGGWCCY 1 cut(s) 948
PspXI VCTCGAGB 1 cut(s) 327
PsrI GAACNNNNNNTAC 2 cut(s) 385, 417
PvuII CAGCTG 1 cut(s) 803
RsaI GTAC 1 cut(s) 171
RsaNI GTAC 1 cut(s) 170
SaqAI TTAA 4 cut(s) 263, 497, 839, 936
SatI GCNGC 7 cut(s) 48, 114, 234, 237, 549, 777, 813
Sau3AI GATC 5 cut(s) 54, 147, 207, 505, 641
Sau96I GGNCC 4 cut(s) 426, 602, 948, 1208
SchI GAGTC 2 cut(s) 446, 1281
ScrFI CCNGG 5 cut(s) 124, 174, 175, 621, 1130
SduI GDGCHC 2 cut(s) 222, 1085
SfaNI GCATC 6 cut(s) 34, 449, 745, 756, 1041, 1234
Sfr274I CTCGAG 1 cut(s) 327
SinI GGWCC 3 cut(s) 426, 602, 948
SlaI CTCGAG 1 cut(s) 327
SmaI CCCGGG 1 cut(s) 175
SmlI CTYRAG 1 cut(s) 327
SmoI CTYRAG 1 cut(s) 327
SphI GCATGC 1 cut(s) 113
Sse9I AATT 3 cut(s) 178, 256, 645
SsiI CCGC 3 cut(s) 198, 923, 1269
SspMI CTAG 4 cut(s) 296, 639, 846, 1266
StyD4I CCNGG 5 cut(s) 122, 172, 173, 619, 1128
StyI CCWWGG 3 cut(s) 333, 1199, 1248
TaaI ACNGT 4 cut(s) 97, 430, 436, 502
TaqI TCGA 2 cut(s) 328, 1094
TasI AATT 3 cut(s) 178, 256, 645
TfiI GAWTC 1 cut(s) 977
Tru1I TTAA 4 cut(s) 263, 497, 839, 936
Tru9I TTAA 4 cut(s) 263, 497, 839, 936
TscAI CASTG 1 cut(s) 1078
TseFI GTSAC 1 cut(s) 430
TseI GCWGC 7 cut(s) 47, 113, 233, 236, 548, 776, 812
Tsp45I GTSAC 1 cut(s) 430
TspDTI ATGAA 2 cut(s) 174, 1102
TspGWI ACGGA 1 cut(s) 599
TspMI CCCGGG 1 cut(s) 173
TspRI CASTG 1 cut(s) 1078
VneI GTGCAC 1 cut(s) 1081
VpaK11BI GGWCC 3 cut(s) 426, 602, 948
XagI CCTNNNNNAGG 1 cut(s) 1203
XapI RAATTY 2 cut(s) 178, 256
XbaI TCTAGA 1 cut(s) 638
XceI RCATGY 2 cut(s) 113, 272
XhoI CTCGAG 1 cut(s) 327
XmaI CCCGGG 1 cut(s) 173
XspI CTAG 4 cut(s) 296, 639, 846, 1266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.