RchiOBHm_Chr4g0434641

protein phosphatase 2C 15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002720
Physical Location & Seq
Forward (+)
58347690 .. 58351189
3500 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40309

Sequence Viewer

Length: 1290 bp
ATGGGGTCTACAGAAGGGAGGCGTAATCATCATAATCTGGTGCCTCTTGCTGCATTGATCAGCAGAGAGATGAAGAATGAGAAGATGGAAAAGCCGACTGTCAGATACGGGCATGCGGCTCAATCCAGGAAAGGGGAGGATTATTTTCTGATCAAGACTGATGGTCAGCGAGTGCCTGGAAATTCTTCATCAACATTTTCTGCATTTGCGATCTTTGACGGGCACAATGGAAACGCAGCAGCCATATATACAAGGGAGAACTTGTTAAATCATGTGATGGGTGCTATTCCCAGGGGGCTAGGAAGGGAGGAGTGGCTCCAAGCCTTACCTCGGGCCTTGGTTGCAGGGTTTGTGAAGGCTGATAAGGATTTCCAAAGTAGAGGAGAAACTTCTGGAACAACAGCTACTGTTGTAATAGTTGATGGATGGACTGTGACAGTAGCATCAGTAGGAGACTCCCGTTGTATCTTAGATACCCAGGGTGGTGCAGTTTCCACTTTAACTGTTGATCATAGACTTGAAGAGAATGTAGAAGAGAGGGAGCGTGTTGCTGCAAGTGGCGGGGAAGTAGGGAGGTTGAGCATTGTTGGTGGTGCGGAGATTGGTCCTCTTCGTTGTTGGCCAGGAGGTTTATGCCTGTCTAGGTCGATTGGAGACATGGATGTTGGGGAGTTCATAGTTCCAATACCATATGTGAAACAAGTCAAGCTATCAAATAGAGGAGGGAGGCTCATAATTGCTTCTGATGGCATCTGGGATGCCCTATCCTCAGAAGAGGCAGCAAAATCTTGCCGTGGGTTGCCCGCTGAACTTGCTGCTAGACAAGTAGTGAAGGAGGCTTTAAGGTCAAGGGGTTTAAAGGATGATACAACCTGTATAGTTGTTGACATAATCCCTCCTGATAACTCGGTACAGCCTACAACTCCCCCAAAAAAGCAGAACAAGCTGAGGGCCCTGTTGTTCAGGAAGAAGTCACGTGATTCTGCTCACAAACTATCCAAGAAGCTATCAGCTGTGGGTATTGTGGAAGAACTATTTGAAGAAGGCTCTGCCATGCTTGCTGAAAGGCTGGGCAATGATGAGTGCACGGCACAATCAACAACATCTGGGCTGTTTATGTGTGCTGTATGCCAAGTTGACCTTGCACCGAGTGAGGGCATATCTGTTCATGCTGGTTCCATCTTCTCCATGAGTTCAAAGCCTTGGCAAGGGCCTTTCCTCTGTTCTGATTGTCGTAATAAGAAGGATGCCATGGAAGGAAAACGCCCTAGCGGAATAAGAGTAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

429

Amino Acids

46.2

Weight (kDa)

8.47

Isoelectric Point (pI)

37.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 61 - 282 6.8e-36 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 40
AccI GTMKAC 1 cut(s) 8
AciI CCGC 5 cut(s) 116, 561, 596, 804, 1272
AcoI YGGCCR 1 cut(s) 620
AcsI RAATTY 1 cut(s) 181
AcvI CACGTG 1 cut(s) 977
AdeI CACNNNGTG 1 cut(s) 1151
AfaI GTAC 1 cut(s) 912
AfiI CCNNNNNNNGG 4 cut(s) 132, 330, 1154, 1208
AgsI TTSAA 3 cut(s) 521, 1040, 1197
AhdI GACNNNNNGTC 1 cut(s) 162
AjnI CCWGG 5 cut(s) 125, 175, 290, 477, 622
AluBI AGCT 5 cut(s) 404, 709, 946, 1006, 1013
AluI AGCT 5 cut(s) 404, 709, 946, 1006, 1013
Alw21I GWGCWC 1 cut(s) 1088
Alw26I GTCTC 2 cut(s) 447, 648
Alw44I GTGCAC 1 cut(s) 1084
AlwNI CAGNNNCTG 1 cut(s) 407
Ama87I CYCGRG 1 cut(s) 330
AoxI GGCC 4 cut(s) 333, 620, 951, 1211
ApaI GGGCCC 1 cut(s) 955
ApaLI GTGCAC 1 cut(s) 1084
ApeKI GCWGC 6 cut(s) 50, 236, 239, 551, 779, 815
ApoI RAATTY 1 cut(s) 181
Asp700I GAANNNNTTC 1 cut(s) 184
AspS9I GGNCC 5 cut(s) 333, 605, 951, 952, 1211
AvaI CYCGRG 1 cut(s) 330
AvaII GGWCC 1 cut(s) 605
BaeGI GKGCMC 3 cut(s) 225, 955, 1088
BalI TGGCCA 1 cut(s) 622
BanI GGYRCC 1 cut(s) 40
BanII GRGCYC 1 cut(s) 955
BbrPI CACGTG 1 cut(s) 977
Bbv12I GWGCWC 1 cut(s) 1088
BbvCI CCTCAGC 1 cut(s) 947
BbvI GCAGC 6 cut(s) 37, 248, 251, 538, 791, 802
BccI CCATC 7 cut(s) 79, 155, 271, 416, 420, 740, 1187
BceAI ACGGC 2 cut(s) 777, 1104
BciT130I CCWGG 5 cut(s) 127, 177, 292, 479, 624
BclI TGATCA 3 cut(s) 57, 150, 508
BcoDI GTCTC 2 cut(s) 447, 648
BfaI CTAG 4 cut(s) 299, 642, 819, 1269
BfmI CTRYAG 1 cut(s) 9
BisI GCNGC 7 cut(s) 51, 117, 237, 240, 552, 780, 816
BlsI GCNGC 7 cut(s) 52, 118, 238, 241, 553, 781, 817
Bme1390I CCNGG 5 cut(s) 127, 177, 292, 479, 624
Bme18I GGWCC 1 cut(s) 605
BmeRI GACNNNNNGTC 1 cut(s) 162
BmeT110I CYCGRG 1 cut(s) 330
BmgT120I GGNCC 5 cut(s) 333, 605, 951, 952, 1211
BmiI GGNNCC 4 cut(s) 42, 317, 953, 1177
BmrFI CCNGG 5 cut(s) 127, 177, 292, 479, 624
BmsI GCATC 4 cut(s) 452, 748, 759, 1237
BplI GAGNNNNNCTC 2 cut(s) 714, 746
Bpu10I CCTNAGC 1 cut(s) 947
BsaAI YACGTR 1 cut(s) 977
BsaJI CCNNGG 9 cut(s) 290, 291, 329, 336, 477, 478, 793, 1202, 1251
BsaXI ACNNNNNCTCC 2 cut(s) 248, 278
Bsc4I CCNNNNNNNGG 4 cut(s) 132, 330, 1154, 1208
Bse3DI GCAATG 1 cut(s) 1081
BseBI CCWGG 5 cut(s) 127, 177, 292, 479, 624
BseDI CCNNGG 9 cut(s) 290, 291, 329, 336, 477, 478, 793, 1202, 1251
BseGI GGATG 5 cut(s) 431, 667, 763, 868, 1252
BseLI CCNNNNNNNGG 4 cut(s) 132, 330, 1154, 1208
BseMI GCAATG 1 cut(s) 1081
BseMII CTCAG 2 cut(s) 783, 938
BseRI GAGGAG 3 cut(s) 323, 396, 735
BseSI GKGCMC 3 cut(s) 225, 955, 1088
BseXI GCAGC 6 cut(s) 37, 248, 251, 538, 791, 802
BseYI CCCAGC 1 cut(s) 1069
BsgI GTGCAG 1 cut(s) 507
BshFI GGCC 4 cut(s) 335, 622, 953, 1213
BshNI GGYRCC 1 cut(s) 40
BsiHKAI GWGCWC 1 cut(s) 1088
BsiHKCI CYCGRG 1 cut(s) 330
BslI CCNNNNNNNGG 4 cut(s) 132, 330, 1154, 1208
BsmAI GTCTC 2 cut(s) 447, 648
BsnI GGCC 4 cut(s) 335, 622, 953, 1213
BsoBI CYCGRG 1 cut(s) 330
Bsp120I GGGCCC 1 cut(s) 951
Bsp1286I GDGCHC 3 cut(s) 225, 955, 1088
Bsp143I GATC 4 cut(s) 57, 150, 210, 508
Bsp19I CCATGG 1 cut(s) 1251
BspACI CCGC 5 cut(s) 116, 561, 596, 804, 1272
BspANI GGCC 4 cut(s) 335, 622, 953, 1213
BspCNI CTCAG 2 cut(s) 782, 939
BspLI GGNNCC 4 cut(s) 42, 317, 953, 1177
BspT107I GGYRCC 1 cut(s) 40
BsrDI GCAATG 1 cut(s) 1081
BssECI CCNNGG 9 cut(s) 290, 291, 329, 336, 477, 478, 793, 1202, 1251
BssMI GATC 4 cut(s) 57, 150, 210, 508
BssT1I CCWWGG 3 cut(s) 336, 1202, 1251
Bst2UI CCWGG 5 cut(s) 127, 177, 292, 479, 624
Bst4CI ACNGT 5 cut(s) 100, 409, 433, 439, 505
Bst6I CTCTTC 4 cut(s) 516, 528, 615, 768
BstBAI YACGTR 1 cut(s) 977
BstC8I GCNNGC 3 cut(s) 114, 804, 1059
BstDEI CTNAG 3 cut(s) 469, 769, 947
BstDSI CCRYGG 2 cut(s) 793, 1251
BstENI CCTNNNNNAGG 1 cut(s) 1206
BstF5I GGATG 5 cut(s) 431, 667, 763, 868, 1252
BstKTI GATC 4 cut(s) 60, 153, 213, 511
BstMAI GTCTC 2 cut(s) 447, 648
BstMBI GATC 4 cut(s) 57, 150, 210, 508
BstMWI GCNNNNNNNGC 4 cut(s) 341, 812, 943, 1058
BstNI CCWGG 5 cut(s) 127, 177, 292, 479, 624
BstNSI RCATGY 1 cut(s) 116
BstSCI CCNGG 5 cut(s) 125, 175, 290, 477, 622
BstSFI CTRYAG 1 cut(s) 9
BstSLI GKGCMC 3 cut(s) 225, 955, 1088
BstV1I GCAGC 6 cut(s) 37, 248, 251, 538, 791, 802
BsuRI GGCC 4 cut(s) 335, 622, 953, 1213
BtgI CCRYGG 2 cut(s) 793, 1251
BtsCI GGATG 5 cut(s) 431, 667, 763, 868, 1252
Cac8I GCNNGC 3 cut(s) 114, 804, 1059
CaiI CAGNNNCTG 1 cut(s) 407
Cfr13I GGNCC 5 cut(s) 333, 605, 951, 952, 1211
Csp6I GTAC 1 cut(s) 911
CviAII CATG 7 cut(s) 113, 272, 658, 1054, 1169, 1189, 1252
CviQI GTAC 1 cut(s) 911
DdeI CTNAG 3 cut(s) 469, 769, 947
DpnI GATC 4 cut(s) 59, 152, 212, 510
DpnII GATC 4 cut(s) 57, 150, 210, 508
DraI TTTAAA 1 cut(s) 858
DraIII CACNNNGTG 1 cut(s) 1151
DriI GACNNNNNGTC 1 cut(s) 162
EaeI YGGCCR 1 cut(s) 620
Eam1104I CTCTTC 4 cut(s) 516, 528, 615, 768
Eam1105I GACNNNNNGTC 1 cut(s) 162
EarI CTCTTC 4 cut(s) 516, 528, 615, 768
Eco130I CCWWGG 3 cut(s) 336, 1202, 1251
Eco24I GRGCYC 1 cut(s) 955
Eco47I GGWCC 1 cut(s) 605
Eco72I CACGTG 1 cut(s) 977
Eco88I CYCGRG 1 cut(s) 330
EcoNI CCTNNNNNAGG 1 cut(s) 1206
EcoO109I RGGNCCY 3 cut(s) 951, 952, 1211
EcoRII CCWGG 5 cut(s) 125, 175, 290, 477, 622
EcoT14I CCWWGG 3 cut(s) 336, 1202, 1251
EcoT38I GRGCYC 1 cut(s) 955
ErhI CCWWGG 3 cut(s) 336, 1202, 1251
FaeI CATG 7 cut(s) 116, 275, 661, 1057, 1172, 1192, 1255
FalI AAGNNNNNCTT 2 cut(s) 1125, 1157
FatI CATG 7 cut(s) 112, 271, 657, 1053, 1168, 1188, 1251
FauI CCCGC 2 cut(s) 554, 811
FauNDI CATATG 1 cut(s) 691
FbaI TGATCA 3 cut(s) 57, 150, 508
FblI GTMKAC 1 cut(s) 8
Fnu4HI GCNGC 7 cut(s) 51, 117, 237, 240, 552, 780, 816
FokI GGATG 5 cut(s) 438, 674, 770, 875, 1259
FriOI GRGCYC 1 cut(s) 955
Fsp4HI GCNGC 7 cut(s) 51, 117, 237, 240, 552, 780, 816
FspBI CTAG 4 cut(s) 299, 642, 819, 1269
GluI GCNGC 7 cut(s) 51, 117, 237, 240, 552, 780, 816
GsaI CCCAGC 1 cut(s) 1073
HaeIII GGCC 4 cut(s) 335, 622, 953, 1213
Hin1II CATG 7 cut(s) 116, 275, 661, 1057, 1172, 1192, 1255
HincII GTYRAC 2 cut(s) 886, 1138
HindII GTYRAC 2 cut(s) 886, 1138
HinfI GANTC 2 cut(s) 455, 980
Hpy166II GTNNAC 4 cut(s) 9, 886, 1086, 1138
Hpy188I TCNGA 5 cut(s) 104, 150, 745, 772, 1228
Hpy188III TCNNGA 4 cut(s) 154, 393, 899, 964
Hpy8I GTNNAC 4 cut(s) 9, 886, 1086, 1138
HpyAV CCTTC 7 cut(s) 8, 297, 349, 826, 1037, 1237, 1250
HpyCH4III ACNGT 5 cut(s) 100, 409, 433, 439, 505
HpyCH4IV ACGT 1 cut(s) 976
HpyCH4V TGCA 7 cut(s) 53, 203, 344, 488, 554, 1086, 1145
HpyF10VI GCNNNNNNNGC 4 cut(s) 341, 812, 943, 1058
HpyF3I CTNAG 3 cut(s) 469, 769, 947
HpySE526I ACGT 1 cut(s) 976
Hsp92II CATG 7 cut(s) 116, 275, 661, 1057, 1172, 1192, 1255
Ksp22I TGATCA 3 cut(s) 57, 150, 508
Kzo9I GATC 4 cut(s) 57, 150, 210, 508
LmnI GCTCC 2 cut(s) 321, 541
Lsp1109I GCAGC 6 cut(s) 37, 248, 251, 538, 791, 802
LweI GCATC 4 cut(s) 452, 748, 759, 1237
MaeI CTAG 4 cut(s) 299, 642, 819, 1269
MaeII ACGT 1 cut(s) 976
MaeIII GTNAC 2 cut(s) 433, 972
MalI GATC 4 cut(s) 59, 152, 212, 510
MboI GATC 4 cut(s) 57, 150, 210, 508
MhlI GDGCHC 3 cut(s) 225, 955, 1088
MlsI TGGCCA 1 cut(s) 622
MluCI AATT 2 cut(s) 181, 735
MluNI TGGCCA 1 cut(s) 622
MlyI GAGTC 1 cut(s) 449
Mox20I TGGCCA 1 cut(s) 622
MroXI GAANNNNTTC 1 cut(s) 184
MscI TGGCCA 1 cut(s) 622
MseI TTAA 4 cut(s) 266, 500, 842, 857
Msp20I TGGCCA 1 cut(s) 622
MspA1I CMGCKG 2 cut(s) 806, 1013
MspR9I CCNGG 5 cut(s) 127, 177, 292, 479, 624
MvaI CCWGG 5 cut(s) 127, 177, 292, 479, 624
MwoI GCNNNNNNNGC 4 cut(s) 341, 812, 943, 1058
NcoI CCATGG 1 cut(s) 1251
NdeI CATATG 1 cut(s) 691
NdeII GATC 4 cut(s) 57, 150, 210, 508
NlaIII CATG 7 cut(s) 116, 275, 661, 1057, 1172, 1192, 1255
NlaIV GGNNCC 4 cut(s) 42, 317, 953, 1177
NmuCI GTSAC 2 cut(s) 433, 972
NspI RCATGY 1 cut(s) 116
PaeI GCATGC 1 cut(s) 116
PasI CCCWGGG 2 cut(s) 291, 478
PdmI GAANNNNTTC 1 cut(s) 184
PfeI GAWTC 1 cut(s) 980
PfoI TCCNGGA 1 cut(s) 125
PkrI GCNGC 7 cut(s) 52, 118, 238, 241, 553, 781, 817
PleI GAGTC 1 cut(s) 449
PmaCI CACGTG 1 cut(s) 977
PmlI CACGTG 1 cut(s) 977
PpsI GAGTC 1 cut(s) 449
Ppu21I YACGTR 1 cut(s) 977
Psp6I CCWGG 5 cut(s) 125, 175, 290, 477, 622
PspCI CACGTG 1 cut(s) 977
PspFI CCCAGC 1 cut(s) 1069
PspGI CCWGG 5 cut(s) 125, 175, 290, 477, 622
PspN4I GGNNCC 4 cut(s) 42, 317, 953, 1177
PspOMI GGGCCC 1 cut(s) 951
PspPI GGNCC 5 cut(s) 333, 605, 951, 952, 1211
PsrI GAACNNNNNNTAC 2 cut(s) 388, 420
PstNI CAGNNNCTG 1 cut(s) 407
PvuII CAGCTG 1 cut(s) 1013
RsaI GTAC 1 cut(s) 912
RsaNI GTAC 1 cut(s) 911
SaqAI TTAA 4 cut(s) 266, 500, 842, 857
SatI GCNGC 7 cut(s) 51, 117, 237, 240, 552, 780, 816
Sau3AI GATC 4 cut(s) 57, 150, 210, 508
Sau96I GGNCC 5 cut(s) 333, 605, 951, 952, 1211
SchI GAGTC 1 cut(s) 449
ScrFI CCNGG 5 cut(s) 127, 177, 292, 479, 624
SduI GDGCHC 3 cut(s) 225, 955, 1088
SfaNI GCATC 4 cut(s) 452, 748, 759, 1237
SfcI CTRYAG 1 cut(s) 9
SinI GGWCC 1 cut(s) 605
SphI GCATGC 1 cut(s) 116
Sse9I AATT 2 cut(s) 181, 735
SsiI CCGC 5 cut(s) 116, 561, 596, 804, 1272
SspMI CTAG 4 cut(s) 299, 642, 819, 1269
StyD4I CCNGG 5 cut(s) 125, 175, 290, 477, 622
StyI CCWWGG 3 cut(s) 336, 1202, 1251
TaaI ACNGT 5 cut(s) 100, 409, 433, 439, 505
TaiI ACGT 1 cut(s) 979
TaqI TCGA 1 cut(s) 647
TasI AATT 2 cut(s) 181, 735
TauI GCSGC 1 cut(s) 119
TfiI GAWTC 1 cut(s) 980
Tru1I TTAA 4 cut(s) 266, 500, 842, 857
Tru9I TTAA 4 cut(s) 266, 500, 842, 857
TseFI GTSAC 2 cut(s) 433, 972
TseI GCWGC 6 cut(s) 50, 236, 239, 551, 779, 815
Tsp45I GTSAC 2 cut(s) 433, 972
TspDTI ATGAA 4 cut(s) 86, 177, 664, 1157
VneI GTGCAC 1 cut(s) 1084
VpaK11BI GGWCC 1 cut(s) 605
XagI CCTNNNNNAGG 1 cut(s) 1206
XapI RAATTY 1 cut(s) 181
XceI RCATGY 1 cut(s) 116
XmiI GTMKAC 1 cut(s) 8
XmnI GAANNNNTTC 1 cut(s) 184
XspI CTAG 4 cut(s) 299, 642, 819, 1269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.